KR950003312A - 연골세포 단백질 - Google Patents
연골세포 단백질 Download PDFInfo
- Publication number
- KR950003312A KR950003312A KR1019940017741A KR19940017741A KR950003312A KR 950003312 A KR950003312 A KR 950003312A KR 1019940017741 A KR1019940017741 A KR 1019940017741A KR 19940017741 A KR19940017741 A KR 19940017741A KR 950003312 A KR950003312 A KR 950003312A
- Authority
- KR
- South Korea
- Prior art keywords
- cmp
- chondrocytes
- concanavalin
- isolating
- protein
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/10—Musculoskeletal or connective tissue disorders
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/84—Bones; tendons; teeth; cartilage
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
본 발명은 연골세포에서는 발견되나 연골세포로부터 탄분화된 세포에서는 발견되지 않는 콘카나발린 A-결합 단백질에 관한 것이다. 이 단백질은 바람직하게는 엔도글리코시다제로 처리한 후 SDS-PAGE 분석에 따른 분자량이 약 76kD 또는 약 67kD이며, 하나 이상의 서열 확인 번호 1 내지 5에 나타난 아미노산 서열을 나타낸다.
본 발명의 단백질은 연골세포의 분화 및 또한 연골세포에 의한 작용 조절 및 연골성 골형성 조절에 관련된 물질이며, 연골대사 질환 및 대사성 골 질환의 치료, 예방 및 진단을 위한 신규한 유형으로서 사용될 수 있다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 콘카나발린 A-결합 당단백질의 전기 영동 양상을 나타낸다. 제2도는 정제된 CMP의 엔도글리코시다제 분해를 나타낸다.
Claims (13)
- 연골세포에는 존재하나 연골세포로부터 탄분화된 세포에는 존재하지 않는 콘카나발린 A결합 단백질(CMP).
- 제1항에 있어서, 엔도글리코시다제로 처리한 후 SDS-PAGE로 분석한 분자량이 약 76kD 또는 약67kD인 단백질.
- 제1항 또는 제2항에 있어서, 서열 확인 번호 1 내지 5에 나타낸 아미노산 서열중 하나 이상을 포함하는 단백질.
- (a)연골세포를 분리시키고, (b) 단계(a)의 연골세포로부터 막 단백질을 분리시킨 후, (c) 콘카나발린 A-친화성 컬럼을 통해 콘카나발린 A-결합 당단백질을 분리시키며, (d) CMP를 분리시켜 수득할 수 있는 CMP.
- 제4항에 있어서, 단계(c)를 수행하기 전에 콘카나발린 A-결합 당단백질 이외의 막 단백질을 분리 시킨 CMP.
- (a)연골세포를 분리시키고, (b) 단계(a)의 연골세포로부터 막 단백질을 분리시킨 후, (c) 콘카나발린 A-친화성 컬럼을 통해 콘카나발린 A-결합 당단백질을 분리시키며, (d) CMP를 분리시키는 단계를 포함하는, CMP 분리 방법.
- 제6항에 있어서, 단계(c)를 수행하기 전에 콘카나발린 A-결합 당단백질 이외의 막 단백질을 분리 시키는 방법.
- CMP를 함유하는 약제.
- CMP에 대한 항체를 함유하는 약제.
- CMP를 함유하는 진단 보조제.
- CMP에 대한 항체를 함유하는 진단 보조제.
- 연골 대사 질환 및/또는 대사성 골 질환의 치료, 예방 및 진단을 위한 CMP의 용도.
- 연골 대사 질환 및/또는 대사성 골 질환의 치료, 예방 및 진단을 위한 CMP에 대한 항체의 용도.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP93-182338 | 1993-07-23 | ||
JP18233893 | 1993-07-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR950003312A true KR950003312A (ko) | 1995-02-16 |
Family
ID=16116566
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019940017741A KR950003312A (ko) | 1993-07-23 | 1994-07-22 | 연골세포 단백질 |
Country Status (7)
Country | Link |
---|---|
US (1) | US5607920A (ko) |
EP (1) | EP0635518B1 (ko) |
KR (1) | KR950003312A (ko) |
AT (1) | ATE251640T1 (ko) |
AU (1) | AU687407B2 (ko) |
CA (1) | CA2128647A1 (ko) |
DE (1) | DE69433221T2 (ko) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5858689A (en) * | 1993-07-22 | 1999-01-12 | Ludwig Institute For Cancer Research | Isolated peptides derived from the gage tumor rejection antigen precursor and uses thereof |
US6008013A (en) * | 1996-07-05 | 1999-12-28 | University Of Rochester | Chondrocyte proteins |
WO2000073417A1 (en) * | 1999-05-27 | 2000-12-07 | The Research Foundation Of State University Of New York | In vitro cell culture device including cartilage and methods of using the same |
EP1219303A4 (en) * | 1999-08-19 | 2003-05-07 | Chugai Pharmaceutical Co Ltd | PROMOTERS OF CHONDROGENESE |
US8697139B2 (en) | 2004-09-21 | 2014-04-15 | Frank M. Phillips | Method of intervertebral disc treatment using articular chondrocyte cells |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3626414C2 (de) * | 1986-08-05 | 1994-11-10 | Robapharm Ag | Präparat zur Stimulierung von Chondrocyten und Osteoblasten (Ossein-Hydroxyapatit-Komplex), Verfahren zu seiner Herstellung und dieses enthaltende Arzneimittel |
JPH05178896A (ja) * | 1990-08-23 | 1993-07-20 | Mitsubishi Kasei Corp | 新規なタンパク質、それをコードする遺伝子及びその産生方法 |
-
1994
- 1994-07-19 EP EP94111218A patent/EP0635518B1/en not_active Expired - Lifetime
- 1994-07-19 AT AT94111218T patent/ATE251640T1/de not_active IP Right Cessation
- 1994-07-19 DE DE69433221T patent/DE69433221T2/de not_active Expired - Fee Related
- 1994-07-21 AU AU68602/94A patent/AU687407B2/en not_active Ceased
- 1994-07-21 US US08/278,617 patent/US5607920A/en not_active Expired - Fee Related
- 1994-07-22 CA CA002128647A patent/CA2128647A1/en not_active Abandoned
- 1994-07-22 KR KR1019940017741A patent/KR950003312A/ko not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
ATE251640T1 (de) | 2003-10-15 |
DE69433221T2 (de) | 2004-07-29 |
AU6860294A (en) | 1995-02-02 |
EP0635518A1 (en) | 1995-01-25 |
EP0635518B1 (en) | 2003-10-08 |
DE69433221D1 (de) | 2003-11-13 |
AU687407B2 (en) | 1998-02-26 |
CA2128647A1 (en) | 1995-01-24 |
US5607920A (en) | 1997-03-04 |
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E902 | Notification of reason for refusal | ||
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