KR930002507A - Method for producing ginkgolides by plant cell culture - Google Patents

Method for producing ginkgolides by plant cell culture Download PDF

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Publication number
KR930002507A
KR930002507A KR1019910012268A KR910012268A KR930002507A KR 930002507 A KR930002507 A KR 930002507A KR 1019910012268 A KR1019910012268 A KR 1019910012268A KR 910012268 A KR910012268 A KR 910012268A KR 930002507 A KR930002507 A KR 930002507A
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South Korea
Prior art keywords
ginkgolides
producing
callus
purifying
separating
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KR1019910012268A
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Korean (ko)
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김영중
진미희
성상현
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김영중
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Priority to KR1019910012268A priority Critical patent/KR930002507A/en
Priority to PCT/KR1992/000031 priority patent/WO1993002204A1/en
Publication of KR930002507A publication Critical patent/KR930002507A/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/22—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains four or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

내용 없음.No content.

Description

식물세포 배양법에 의한 징콜라이드류의 제조방법Method for producing ginkgolides by plant cell culture

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제 9-1도는 표준물질 징코라이드 A(GKA), B(GKB) 및 C(GKS)의 가스크로 마토그램을 나타낸 것이며,Figure 9-1 shows the gas chromatogram of the standards ginkgoride A (GKA), B (GKB) and C (GKS),

제 9-2도는 표준물질 징코라이드 B(GKB)의 농도에 따른 가스크로마토그램을 나타낸 것이며,Figure 9-2 shows the gas chromatogram according to the concentration of the standard ginkgoride B (GKB),

제 9-3도는 현탁 배양한 뿌리 세포로부터 분리한 징코라이드의 가스크로 마토그램을 나타낸 것이며 (여기에서 A : 현탁 배양한 뿌리 세포로부터 분리한 징코라이드에 표준물질 GKB를 첨가한 가스크로마토그램이며, B : 현탁 배양한 뿌리 세포로부터 분리한 징코라이드의 가스크로마토그램이며, C : 표준물질 징코라이드 B(GKB)의 가스크로마토그램이다.)9-3 shows gas chromatograms of ginkgolides isolated from suspension cultured root cells (here: A: gas chromatograms with standard GKB added to ginkgorides isolated from suspension cultured root cells, B: Gas chromatogram of ginkgolide isolated from suspension cultured root cells, C: Gas chromatogram of standard ginkgoride B (GKB).)

제9-4도는 은행잎으로부터 유기된 칼루스로부터 분리된 징코라이드의 가스크로마토그램을 나타낸 것이다.9-4 show gas chromatograms of ginkgolides isolated from callus, which is derived from ginkgo biloba leaves.

Claims (4)

은행나무의 식물세포를 통상의 식물세포를 배양하는 배지에서 배양하여 얻어진 칼루스 및/또는 현탁배양한 세포 및/또는 배양액으로부터 징코라이드류를 분리 정제하여 징코라이드류를 제조하는 방법.A method for producing ginkgo lysates by separating and purifying ginkgo lysates from callus and / or suspension cultured cells and / or culture obtained by cultivating plant cells of ginkgo biloba in a medium for cultivating normal plant cells. 제1항에서 배양액에 성장조절물질로서 1-나프탈렌초산(NAA), 3-인돌초산(IAA), 3-인돌부티르산(IBA), 2,4-디클로로페녹시초산(2,4-D)등과 같은 옥신류 또는 6-벤질아므노퓨린 또는 키네틴등과 같은 사이 토키닌류등으로 이루어진 그룹으로부터 선택된 1종이상의 성장조절물질을 배양액에 첨가하고 배양하여 얻어진 칼루스 및/또는 현탁배양한 세포 및/또는 배양액으로부터 징코라이드류를 분리정제하여 징코라이드류를 제조하는 방법.The culture medium according to claim 1 as a growth regulator, 1-naphthalene acetate (NAA), 3-indole acetate (IAA), 3-indolebutyric acid (IBA), 2,4-dichlorophenoxy acetate (2,4-D) and the like Callus and / or suspension cultured cells and / or cultures obtained by adding and culturing at least one growth regulator selected from the group consisting of auxins or cytokinins such as 6-benzylamnopurine or kinetin Method for preparing ginkgorides by separating and purifying the ginkgorides from. 제1항 또는 2항에서 배양액에 활성탄을 첨가하고 배양하여 얻어진 칼루스 및/또는 현탁배양한 세포 및/또는 배양액으로 부터 징코라이드를 분리 정제하여 징코라이드류를 제조하는 방법.A method for producing ginkgolides by separating and purifying ginkgolides from callus and / or suspension cultured cells and / or cultures obtained by adding activated carbon to the culture solution according to claim 1 or 2. 제1항 또는 2항에서 사용한 배양액의 조성울 변화시키거나 또는 징코라이드 생합성의 전구물질, (예를들면 : 메발로닌산, 메치오닌 등)의 첨가 및 생합성에 관여하는 효소의 증가시킬 수 있는 물질을 첨가하고 배양하여 징코라이드의 수율을 높이는 방법.A substance capable of changing the composition of the culture medium used in Claims 1 or 2, or adding a precursor of jinkoride biosynthesis, such as mevalonic acid, methionine, etc. and increasing the enzymes involved in biosynthesis. Adding and culturing to increase the yield of ginkgoride. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910012268A 1991-07-18 1991-07-18 Method for producing ginkgolides by plant cell culture KR930002507A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1019910012268A KR930002507A (en) 1991-07-18 1991-07-18 Method for producing ginkgolides by plant cell culture
PCT/KR1992/000031 WO1993002204A1 (en) 1991-07-18 1992-07-16 Production of ginkgolides in cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910012268A KR930002507A (en) 1991-07-18 1991-07-18 Method for producing ginkgolides by plant cell culture

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KR930002507A true KR930002507A (en) 1993-02-23

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WO (1) WO1993002204A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE343393T1 (en) * 2001-07-11 2006-11-15 Univ Columbia METHOD FOR ISOLATION OF TERPENTRILACTONES (GINKGOLIDS, BILOBALIDS) FROM LEAVES AND PHARMACEUTICAL POWDER FROM GINKGO BILOBA
US7145021B2 (en) 2002-03-29 2006-12-05 The Trustees Of Columbia University In The City Of New York Analogs of terpene trilactones from Ginkgo biloba and related compounds and uses thereof
US7429670B2 (en) 2003-08-27 2008-09-30 The Trustees Of Columbia University In The City Of New York Synthesis of derivatives of ginkgolide C
WO2005046829A2 (en) 2003-11-12 2005-05-26 The Trustees Of Columbia University In The City Of New York Separation of ginkgolides and bilobalide from g. biloba
JP2012527889A (en) * 2009-05-26 2012-11-12 株式会社ウンファ Plant stem cells derived from the formation layer of Ginkgoaceae
CN102464666B (en) * 2010-11-09 2015-04-08 上海医药工业研究院 Preparation method for ginkgolide C
CN102464665B (en) * 2010-11-09 2015-04-08 上海医药工业研究院 Method for preparing ginkgolide A
CN110291989B (en) * 2019-07-29 2022-03-22 南京林业大学 Establishment method of sterile leaf source for inducing ginkgo callus
CN114375839B (en) * 2022-01-30 2022-09-13 山东大丰园农业有限公司 Ginkgo regeneration method based on leaf induction

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3726864A1 (en) * 1987-08-12 1989-02-23 Bauer Johann USE OF GINKGO BILOBAE EXTRACTS FOR COMBATING INFLAMMABLE PROCESSES
KR940002795B1 (en) * 1989-06-16 1994-04-02 주식회사 선경인더스트리 Separation and purification in ginkgo
DE3940092A1 (en) * 1989-12-04 1991-06-06 Schwabe Willmar Gmbh & Co EXTRACT OF BLACKERS OF GINKGO BILOBA, METHOD FOR THE PRODUCTION THEREOF AND THE EXTRACT CONTAINING MEDICAMENT

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