KR927003623A - 변성단백질의 활성회복 방법 - Google Patents

변성단백질의 활성회복 방법 Download PDF

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KR927003623A
KR927003623A KR1019920701705A KR920701705A KR927003623A KR 927003623 A KR927003623 A KR 927003623A KR 1019920701705 A KR1019920701705 A KR 1019920701705A KR 920701705 A KR920701705 A KR 920701705A KR 927003623 A KR927003623 A KR 927003623A
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protein
tris
solution
treated
concentration
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KR1019920701705A
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KR960013385B1 (ko
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암브로시우스 도로테아
루돌프 라이너
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포우크베트ㆍ베버
뵈링거 만하임 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

변성단백질의 활성회복 방법
[도면의 간단한 설명]
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 그 처리될 단백질이 고유형태를 취할 수 있는 pH를 지니고, 농도가 최소한 400mmol/1인 트리스 염기 또는/및 트리스 염으로서 이루어지는 용액으로 단백질이 배양(培養)되는 변성단백질의 복원방법.
  2. 제1항에 있어서, 그 배양용액이 pH값이 트리스와는 상이한 완충물질에 의해 조정되어지는 방법.
  3. 제1항에 있어서, 트리스 또한 그 pH값을 조정키위해 사용되어지는 방법.
  4. 전기한 항중 어느 한 항에 있어서, 0.4에서 2mol/1 까지의 트리스 용액이 사용되어지는 방법.
  5. 전기한 항중 어느 한 항에 있어서, 0.2에서 1.0mol/1 까지의 일기닌이 그 배양용액에 첨가되어지는 방법.
  6. 제5항에 있어서, 제조합 tPA 또는 tPA돌연변이 단백질이 처리되는 방법.
  7. 제6항에 있어서, 영역조성 K2P(domain composition K2P)를 지니는 비글리코실화된 tPA 돌연변이 단백질(non-glycosylated tPA mutein)이 처리되는 방법.
  8. 전기한 항중 어느 한 항에 있어서, 그 변성단백질이 연속식으로 또는 회분식(回分式)으로 그 복원용액에 첨가되는 방법.
  9. 제8항에 있어서, 펄스복원 방법이 복원반응기(renaturation reactor)에서 행해지는 방법.
  10. 제9항에 있어서, 그 복원이 약1mol/1의 트리스 농도를 지니는 알기닌 함유완충액에서 행해지고, 그리고 그 단백질 농도가 펄스당 약 200μmg까지 증가되어지는 방법.
  11. 제1항 내지 5항 또는 제8항 내지 제10항중 어느 한 항에 있어서, 재조합 G-CSF가 처리되는 방법.
  12. 제1항 내지 5항 또는 제8항 내지 10항중 어느 한 항에 있어서, 항체들 또는 그들의 단편들이 처리되는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920701705A 1990-11-22 1991-11-21 변성 단백질의 활성회복 방법 KR960013385B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP4037196.4 1990-11-22
DE4037196A DE4037196A1 (de) 1990-11-22 1990-11-22 Verfahren zur reaktivierung von denaturiertem protein
PCT/EP1991/002190 WO1992009622A1 (de) 1990-11-22 1991-11-21 Verfahren zur reaktivierung von denaturiertem protein

Publications (2)

Publication Number Publication Date
KR927003623A true KR927003623A (ko) 1992-12-18
KR960013385B1 KR960013385B1 (ko) 1996-10-04

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KR1019920701705A KR960013385B1 (ko) 1990-11-22 1991-11-21 변성 단백질의 활성회복 방법

Country Status (17)

Country Link
US (1) US5618927A (ko)
EP (1) EP0512087B1 (ko)
JP (1) JP2572517B2 (ko)
KR (1) KR960013385B1 (ko)
AT (1) ATE149514T1 (ko)
AU (1) AU8919591A (ko)
CA (1) CA2074182C (ko)
DE (2) DE4037196A1 (ko)
DK (1) DK0512087T3 (ko)
ES (1) ES2098379T3 (ko)
GR (1) GR3023110T3 (ko)
HU (1) HU213589B (ko)
IE (1) IE62052B1 (ko)
PT (1) PT99578B (ko)
RU (1) RU2070888C1 (ko)
WO (1) WO1992009622A1 (ko)
ZA (1) ZA919207B (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL109149A0 (en) * 1993-04-01 1994-06-24 Schering Corp In vitro refolding of recombinant fv fragments
US20030133905A1 (en) * 2001-12-20 2003-07-17 Zhenze Hu Composition for treating contact lenses in the eye
SI21273A (sl) * 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
US20080125580A1 (en) * 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
US20160060291A1 (en) * 2012-03-30 2016-03-03 Biogenomics Limited Process for renaturation of polypeptides
WO2014167574A1 (en) * 2013-03-22 2014-10-16 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins
RU2646103C2 (ru) * 2016-03-30 2018-03-01 Федеральное бюджетное учреждение науки Государственный научный центр прикладной микробиологии и биотехнологии (ФБУН ГНЦ ПМБ) Способ выделения ренатурированного белка g из маркированного полиакриламидного геля

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat

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Publication number Publication date
ZA919207B (en) 1992-08-26
RU2070888C1 (ru) 1996-12-27
EP0512087B1 (de) 1997-03-05
EP0512087A1 (de) 1992-11-11
GR3023110T3 (en) 1997-07-30
CA2074182A1 (en) 1992-05-23
IE62052B1 (en) 1994-12-14
IE913474A1 (en) 1992-06-03
US5618927A (en) 1997-04-08
HUT63437A (en) 1993-08-30
CA2074182C (en) 1999-10-05
JP2572517B2 (ja) 1997-01-16
WO1992009622A1 (de) 1992-06-11
KR960013385B1 (ko) 1996-10-04
JPH05502245A (ja) 1993-04-22
ATE149514T1 (de) 1997-03-15
AU8919591A (en) 1992-06-25
DE59108583D1 (de) 1997-04-10
DK0512087T3 (da) 1997-08-25
PT99578A (pt) 1992-10-30
PT99578B (pt) 1999-04-30
DE4037196A1 (de) 1992-05-27
HU213589B (en) 1997-08-28
ES2098379T3 (es) 1997-05-01
HU9202354D0 (en) 1992-10-28

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