KR900700615A - 사람 호중구 화학주성 인자 폴리펩티드의 제조방법 - Google Patents
사람 호중구 화학주성 인자 폴리펩티드의 제조방법Info
- Publication number
- KR900700615A KR900700615A KR1019890702514A KR890702514A KR900700615A KR 900700615 A KR900700615 A KR 900700615A KR 1019890702514 A KR1019890702514 A KR 1019890702514A KR 890702514 A KR890702514 A KR 890702514A KR 900700615 A KR900700615 A KR 900700615A
- Authority
- KR
- South Korea
- Prior art keywords
- dna
- sequence represented
- nucleotide sequence
- formula
- chemotactic factor
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 7
- 108090001007 Interleukin-8 Proteins 0.000 title claims 5
- 102000004890 Interleukin-8 Human genes 0.000 title claims 5
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- 150000001413 amino acids Chemical group 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000006378 damage Effects 0.000 claims 1
- 238000002523 gelfiltration Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 238000005185 salting out Methods 0.000 claims 1
- -1 salting out Chemical class 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5421—IL-8
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (10)
- 사람 호중구 화학주성 인자 폴리펩티드의 제조방법으로서, 상기 폴리펩티드를 코드화하는 DNA가 삽입되어 있는 발현벡터로 형질전환된 세포를 배양함에 의한 방법.
- 제1항에 있어서, 사람 호중구 화학주성 인자 폴리펩티드가 다음식[I]에 의해 표시되는 아미노산 배열로 이루어지는 폴리펩티드인 것을 특징으로 하는 방법.
- 제1항에 있어서, 사람 호중구 화학주성 인자 폴리펩티드를 코드화하는 DNA가 다음식 [A]로 표시되는 염기배열, 또는 그의 축퇴성 배열로 이루어지는 DNA인 것을 특징으로 하는 방법.
- 제1항에 있어서, 형질전환된 세포가 대장균인 것을 특징으로 하는 방법.
- 제1항에 있어서, 발현벡터로서 재조합 플라스미드 pHNP101을 사용하는 것을 특징으로 하는 방법.
- 제3항에 있어서, 도시된 식[A]로 표시되는 염기 배열 및 상기 식[A]로 표시된 염기배열 위쪽에 다음식 [B]로 표시되는 염기배열을 가지고 있는 DNA.5′-XGGAGGTTTYATTATG-3′ 식 [B](상기식에서, X는 (A)X를 의미하고, x는 1내지 5이며, Y는 (A)y(T)Z를 의미하고, y는 0 내지 3이며 z는 0또는 1이다.)
- 제3항에 도시된 식[A]로 표시되는 염기배열 및 상기 식[A]로 표시된 염기배열의 위쪽에 다음식[A]로 표시된 염기배열을 가지고 있는 DNA.5′-AAAAGGAGGTTTAAATTATG-3' 식 [C]
- 제6항 또는 7항에 있어서, 제6항 또는 7항의 DNA의 위쪽에 대장균에서의 전사를 제어하는 프로모터활성을 활성을 가지고 있는 DNA가 추가로 연결되어 있는 거을 특징으로 하는 DNA.
- 제8항에 있어서, 프로모터가 트립토판 오페론의 프로모터인 것을 특징으로 하는 DNA.
- 제1항 내지 5항중 어느 하나의 방법에 의하여 제조되는 사람 호중구 화학주성 인자폴리펩티드의 정제방법에 있어서, 상기 폴리펩티드의 처리에 있어서 다음 단계, 형질전환체의 파괴, 핵산을 제거하기 위한 처리, 염석, 음이온 및/또는 양이온 교환 크로마토그래피, 한외여과, 및 겔 여과의 조합에 의하여 이루어지는 것을 특징으로 하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18916488A | 1988-05-02 | 1988-05-02 | |
US189,164 | 1988-05-02 | ||
PCT/JP1989/000437 WO1989010962A1 (en) | 1988-05-02 | 1989-04-26 | Process for producing a human neutrophil chemotactic factor polypeptide |
Publications (1)
Publication Number | Publication Date |
---|---|
KR900700615A true KR900700615A (ko) | 1990-08-16 |
Family
ID=22696203
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890702514A KR900700615A (ko) | 1988-05-02 | 1989-04-26 | 사람 호중구 화학주성 인자 폴리펩티드의 제조방법 |
Country Status (7)
Country | Link |
---|---|
US (1) | US5306627A (ko) |
EP (1) | EP0413818B1 (ko) |
JP (1) | JPH03505037A (ko) |
KR (1) | KR900700615A (ko) |
AT (1) | ATE116366T1 (ko) |
DE (1) | DE68920321T2 (ko) |
WO (1) | WO1989010962A1 (ko) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2223225B (en) | 1987-11-19 | 1992-07-08 | Sandoz Ltd | Neutrophil-activating factor: its production and use in treatment |
US5652338A (en) | 1988-03-16 | 1997-07-29 | The United States Of America As Represented By The Department Of Health And Human Services | Neutrophil chemotactic factor |
EP0504257A4 (en) * | 1989-11-29 | 1993-03-03 | Brigham And Women's Hospital | (ala il-8) 77 as a leukocyte adhesion inhibitor |
US5241049A (en) * | 1989-12-22 | 1993-08-31 | Zymogenetics, Inc. | Neutrophil chemoattractants |
WO2004058797A2 (en) | 2002-12-16 | 2004-07-15 | Medarex, Inc. | Human monoclonal antibodies against interleukin 8 (il-8) |
JP2007006419A (ja) * | 2005-06-27 | 2007-01-11 | Pioneer Electronic Corp | スピーカ用ダンパ、スピーカおよびスピーカの組立方法 |
KR20200108868A (ko) | 2018-01-12 | 2020-09-21 | 브리스톨-마이어스 스큅 컴퍼니 | 암을 치료하기 위한 항-il-8 항체 및 항-pd-1 항체와의 조합 요법 |
-
1989
- 1989-04-26 WO PCT/JP1989/000437 patent/WO1989010962A1/en active IP Right Grant
- 1989-04-26 DE DE68920321T patent/DE68920321T2/de not_active Expired - Lifetime
- 1989-04-26 AT AT89905179T patent/ATE116366T1/de not_active IP Right Cessation
- 1989-04-26 EP EP89905179A patent/EP0413818B1/en not_active Expired - Lifetime
- 1989-04-26 KR KR1019890702514A patent/KR900700615A/ko not_active Application Discontinuation
- 1989-04-26 JP JP1504941A patent/JPH03505037A/ja active Pending
-
1991
- 1991-06-04 US US07/711,275 patent/US5306627A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ATE116366T1 (de) | 1995-01-15 |
JPH03505037A (ja) | 1991-11-07 |
EP0413818A1 (en) | 1991-02-27 |
EP0413818B1 (en) | 1994-12-28 |
WO1989010962A1 (en) | 1989-11-16 |
DE68920321T2 (de) | 1995-05-24 |
US5306627A (en) | 1994-04-26 |
DE68920321D1 (de) | 1995-02-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Sherry et al. | Identification of cyclophilin as a proinflammatory secretory product of lipopolysaccharide-activated macrophages. | |
IE811463L (en) | Interferons | |
US5359035A (en) | Bifunctional proteins including interleukin-2 (IL-2) and granuloctyte macrophage colony stimulating factor (GM-CSF) | |
ATE80180T1 (de) | Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''. | |
IL173399A0 (en) | Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy | |
KR880701288A (ko) | M-csf의 제법 | |
NO974812L (no) | Nytt protein og fremgangsmåte for fremstilling av dette | |
BR8107293A (pt) | Polipetidio anti-viral dna de duplo cordao veiculo de expressao plasmidico replicavel microorganismo transformado plasmidio processo de formacao do dito dna e do dito polipeptidio composicao farmaceutica e uso do dito polipeptidio | |
ATE26463T1 (de) | Verfahren zur herstellung von menschlichem leukozyten-interferon. | |
DK164940C (da) | Polypeptid med human-gamma-interferonaktivitet, genteknologisk fremgangsmaade til fremstilling deraf, hybrid-plasmider indeholdende en dna-sekvens, der koder for peptidet, vaertsorganismer indeholdende hybridplasmiderne, samt laegemidler, som indeholder polypeptidet | |
NZ224247A (en) | Bridged protein containing il-2 and gm-csf moities | |
MX26714A (es) | Procedimiento para la preparacion de albumina de SUERO HUMANO VECTOR Y PLASMIDO USADOS ASI COMO LEVADURA METILOTROFICA TRANSFORMADA OBTENIDA | |
PT95923A (pt) | Processo para a preparacao de complexos proteicos possuindo actividade do factor viii.c | |
KR970701199A (ko) | 성장 정지 호메오박스 유전자(Growth Arrest Homeobox Gene) | |
DK163937B (da) | Strukturelt gen, som koder for autentisk humant serumalbumin til ekspression i en ikke-human vaert, fremgangsmaade til fremstilling deraf, rekombinant-dna-molekyle indeholdende genet, vaertsorganisme transformeret dermed og fremgangsmaade til fremstilling af autentisk humant serumalbumin | |
KR900014428A (ko) | 신규폴리펩타이드 및 그 제조방법 | |
KR900700615A (ko) | 사람 호중구 화학주성 인자 폴리펩티드의 제조방법 | |
AU2795989A (en) | Production of gonorrheal pi proteins and vaccines | |
ATE89317T1 (de) | Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten. | |
KR900016465A (ko) | 사람의 macif 활성을 지닌 단백질을 암호화하는 유전자, 이 유전자를 함유하는 발현벡터, 형질전환 세포 및 사람 macif활성을 갖는 단백질 | |
WO1990004035A1 (en) | Recombinant pdgf and methods for production | |
EP0072541A3 (en) | Human leukocyte interferons, process for their microbial production, intermediates therefor and compositions containing them | |
GB2171703B (en) | Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re | |
DK0805204T3 (da) | Bitestikel - specifikt receptorprotein og anvendelse heraf | |
EP0276778A3 (en) | High level expression in e. coli of soluble mature hil-1beta and derivatives with altered biological activity |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |