KR860007278A - 천연 헤파란 설페이트 및 더머탄 설페이트의 제조방법 - Google Patents

천연 헤파란 설페이트 및 더머탄 설페이트의 제조방법 Download PDF

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KR860007278A
KR860007278A KR1019860001805A KR860001805A KR860007278A KR 860007278 A KR860007278 A KR 860007278A KR 1019860001805 A KR1019860001805 A KR 1019860001805A KR 860001805 A KR860001805 A KR 860001805A KR 860007278 A KR860007278 A KR 860007278A
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solution
sulfate
carried out
concentration
demertan
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KR1019860001805A
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KR910006809B1 (ko
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델 보노 리날도
드 암브로시 루이지
파젤라 피에르쥬세페
페라리 지안니
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메디올라눔 파마수티시 에스알엘
원본미기재
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0066—Isolation or extraction of proteoglycans from organs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
    • C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
    • C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Dermatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Diabetes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Steroid Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

천연 헤파란 설페이트 및 더머탄 설페이트의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 미분화된 형태의, 동물의 대동맥, 심근층 및 특히 혈관화된 기관 조직을 우레아 또는 기타 유사물질의 용액으로 처리하여 프로테오글리칸을 추출하고 :
    이 용액을 여과하고 정화시켜, 우레아 또는 기타 유사물질을 제거하며 :
    뮤코 다당류와 단백질 사이의 결합을 분리시키고 :
    단백질을 침전시켜 여과하고 :
    미량의 핵산을 제거하며 :
    뮤코 다당류를 침전시키고 :
    헤파란 설페이트 및 더머탄 설페이트를 분별하고 정제시킴을 특징으로 하여, 상기 동물 조직의 프로테오글리칸 혼합물로부터 상당히 순수한 형태의 천연 헤파란 설페이트 및 더머탄 설페이트를 제조하는 방법.
  2. 제 1 항에 있어서, 상기 프로데오글리칸 추출공정을, 상기 조직을 0.5 내지 2중량 %의 아세트산 나트륨, 0.2 내지 1 중량%의 EDTA 나트륨염 및 20 내지 30중량 %의 우레아를 함유하는 탈염수 용액으로 상기 조직에 대한 용액의 중량비가 2 내지 4가 되도록 하여, 15내지 50시간동안 주변온도에서 교반시키면서 처리함으로써 수행함을 특징으로 하는 방법.
  3. 제 1 항에 있어서, 상기 우레아 또는 기타 유사물질이 구아니딘, 티오우레아 및 칼륨 티오시아네이트로 이루어짐을 특징으로 하는 방법.
  4. 제 1 항에 있어서, 상기 우레아 또는 기타 유사물질의 제거공정을, 우레아 또는 기타 유사물질의 함량이 5중량 % 미만인 용액이 수득될때까지, 계속 반복하여, 탈염수로 희석시키고 한외여과로 농축시켜 수행함을 특징으로 하는 방법.
  5. 제 1 항에 있어서, 뮤코다당류와 단백질 사이의 결합을 분리시키는 공정을, 선행단계의 용액을 5 내지 15시간 동안 주변온도에서 최종 NaCl 농도가 1.5 내지 2.5M이 되도록 하는 양의, 8.7 내지 14.5중량 % 농도의 염화나트륨 용액으로 처리하여 수행함을 특징으로 하는 방법.
  6. 제 1 항에 있어서, 상기 단백질 침전공정을, 3 내지 6중량 % 농도의 트리클로로아세트산으로 처리하여 수행함을 특징으로 하는 방법.
  7. 제 1 항에 있어서, 상기 핵산 제거공정을, 2시간 동안 주변온도에서 교반시키면서 pH 3내지 4에서 용액에 대해 2 내지 5중량 % 양의 활성화토류로 처리하여 수행함을 특징으로 하는 방법.
  8. 제 1 항에 있어서, 상기 뮤코다당류 침전 공정을, pH 5 내지 6에서 용액에 대해 0.8 : 1 내지 1.2 : 1 용적비의 알코올 또는 케톤(예를 들면 메탄올, 에탄올 또는 아세톤)으로 처리하여 수행함을 특징으로 하는 방법.
  9. 제 1 항에 있어서, 상기 헤파란 설페이트 및 더머탄 설페이트의 분별 공정을, 헤파란 설페이트 및 더머탄 설페이트를 함유하는 조생성물을 탈염수에 용해시켜 1 내지 3중량 %의 농도로 만들고, 양이온성 수지로 처리한 후, 수산화 칼슘으로 중화시키고, 먼저 30용적 %의 농도까지, 그후 50용적 %의 농도까지의 아세톤을 가하여 분별 침전시켜 수행함을 특징으로 하는 방법.
  10. 제 1 항에 있어서, 상기 헤파란 설페이트 및 더머탄 설페이트의 정제 공정을, 두 조생성물을, 용액중 농도가 3 내지 7중량 %로 되도록 하는 양의 2M 염화나트륨 용액에 각각 따로따로 재용해시키고, 여과한 후, 용액에 대해 0.8 : 2 내지 1.2 : 2 용적비의 메탄올을 가하여 순수한 생성물을 재침전시켜 수행함을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860001805A 1985-03-13 1986-03-13 천연 헤파란 설페이트 및 더머탄 설페이트의 제조방법 KR910006809B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT8519885A IT1208509B (it) 1985-03-13 1985-03-13 Processo per la produzione di eparan solfato e dermatan solfato naturali sostanzialmente puri eloro impiego farmaceutico.
IT19885A/85 1985-03-13

Publications (2)

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KR860007278A true KR860007278A (ko) 1986-10-10
KR910006809B1 KR910006809B1 (ko) 1991-09-02

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Country Status (20)

Country Link
US (1) US4783447A (ko)
EP (1) EP0199033B1 (ko)
JP (1) JPS61218601A (ko)
KR (1) KR910006809B1 (ko)
AT (1) ATE99702T1 (ko)
AU (1) AU582221B2 (ko)
CA (1) CA1286286C (ko)
DE (1) DE3689493T2 (ko)
DK (1) DK113886A (ko)
ES (1) ES8705894A1 (ko)
FI (1) FI82700C (ko)
GR (1) GR860677B (ko)
IN (1) IN163616B (ko)
IT (1) IT1208509B (ko)
NO (1) NO166187C (ko)
NZ (1) NZ215435A (ko)
PH (2) PH22370A (ko)
PT (1) PT82198B (ko)
YU (1) YU46303B (ko)
ZA (1) ZA861855B (ko)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1189085B (it) * 1986-03-25 1988-01-28 Mediolanum Farmaceutici Srl Processo per la preparazione di dermatan solfato ad alta purezza e composizioni farmaceutiche che lo contengono
US5707604A (en) * 1986-11-18 1998-01-13 Access Pharmaceuticals, Inc. Vivo agents comprising metal-ion chelates with acidic saccharides and glycosaminoglycans, giving improved site-selective localization, uptake mechanism, sensitivity and kinetic-spatial profiles
DE3639561A1 (de) * 1986-11-20 1988-06-01 Baumann Hanno Verfahren zur herstellung von nicht-thrombogenen substraten
IT1232939B (it) * 1987-11-06 1992-03-10 Opocrin Spa Eparan solfato caratterizzato da elevata attivita' antitrombotica, elevata biodisponibilita', assenza di attivita' anticoagulante; suo processo di estrazione da organi e da tessuti e relative composizioni farmaceutiche
WO1989012070A1 (en) * 1988-06-03 1989-12-14 Italfarmaco S.P.A. Glycosaminoglycan salts, processes for the preparation thereof and pharmaceutical compositions containing them
EP0421508B1 (en) * 1989-10-04 1993-12-15 Akzo Nobel N.V. Sulphated glycosaminoglycuronan with antithrombotic activity
WO1991006303A1 (en) * 1989-10-27 1991-05-16 Case Western Reserve University Inhibition of cell growth by keratan sulfate, chondroitin sulfate, dermatan sulfate and other glycans
IT1240615B (it) * 1990-04-03 1993-12-17 Mediolanum Farmaceutici Spa Impiego del dermatan solfato nella prevenzione e terapia dell'invecchiamento cerebrale e degli stati di deficit funzionale del sistema nervoso centrale
WO1991015217A1 (en) * 1990-04-06 1991-10-17 Washington University Anticoagulant oligosaccharides
US5705178A (en) * 1991-05-31 1998-01-06 Gliatech, Inc. Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers
US5605938A (en) * 1991-05-31 1997-02-25 Gliatech, Inc. Methods and compositions for inhibition of cell invasion and fibrosis using dextran sulfate
FR2691066B1 (fr) * 1992-05-15 1995-06-09 Sanofi Elf Utilisation de glycosaminoglycanes exogenes, de leurs analogues ainsi que de leurs fragments, fractions et derives, pour la preparation de medicaments destines au traitement de thrombopenies.
IT1256236B (it) * 1992-12-23 1995-11-29 Mediolanum Farmaceutici Srl Oligosaccaridi aventi attivita' biologica e procedimento per la loro preparazione di glicosaminoglicani
WO1995009188A1 (fr) * 1993-09-30 1995-04-06 Seikagaku Kogyo Kabushiki Kaisha Antithrombotique
US6106866A (en) * 1995-07-31 2000-08-22 Access Pharmaceuticals, Inc. In vivo agents comprising cationic drugs, peptides and metal chelators with acidic saccharides and glycosaminoglycans, giving improved site-selective localization, uptake mechanism, sensitivity and kinetic-spatial profiles, including tumor sites
IT1296581B1 (it) * 1997-11-28 1999-07-14 Istituto Scient Di Chimica E B Materiali polimerici non trombogenici e ad esaltata compatibilita' con fluidi e tessuti organici
WO2001002443A1 (en) * 1999-06-30 2001-01-11 Hamilton Civic Hospitals Research Development, Inc Heparin compositions that inhibit clot associated coagulation factors
AU2001291549A1 (en) * 2000-09-08 2002-03-22 Hamilton Civic Hospitals Research Development Inc. Antithrombotic compositions
CN107098989A (zh) * 2017-04-27 2017-08-29 甘肃省金羚集团药业有限公司 一种肝素钠的制备方法
CN114478832B (zh) * 2022-02-14 2023-03-03 河北常山生化药业股份有限公司 从肝素钠副产物中提纯四种多糖类产品的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3179566A (en) * 1963-05-16 1965-04-20 Canada Packers Ltd Purification of heparin
JPS5318520B2 (ko) * 1972-07-05 1978-06-15
AR207237A1 (es) * 1974-02-25 1976-09-22 Thomas A Procedimiento para obtener extractos biologicos estables liofilizados solubles constituidos por complejos proteicos termorresistentes
US4243582A (en) * 1979-04-26 1981-01-06 Monsanto Company Novel glycoproteins from bovine cartilage
JPS58171403A (ja) * 1982-04-01 1983-10-08 Eisai Co Ltd ヘパラン硫酸の精製法
IT1189298B (it) * 1982-06-18 1988-02-04 Manetti & Roberts Italo Brit Procedimento per produrre i glicosa minoglicani dermatansolfato ed eparan-solfato sostanzialmente puri e loro impiego farmaceutico
FR2555993B1 (fr) * 1983-12-06 1986-11-07 Anic Spa Chitosanes 6-sulfates et procede pour leur preparation
IT1195497B (it) * 1983-03-08 1988-10-19 Opocrin Spa Procedimento per la preparazione di frazioni oligosaccaridiche dotate di proprieta' farmacologiche per degradazione chimica di eparina
IT1163772B (it) * 1983-07-13 1987-04-08 Baldacci Lab Spa Oligosaccaridi eterogenei complessabili ed alfa-idosani ad attivita' terapeutica, procedimento per la loro preparazione e relative composizioni farmaceutiche

Also Published As

Publication number Publication date
PH25136A (en) 1991-02-19
US4783447A (en) 1988-11-08
IN163616B (ko) 1988-10-15
PT82198A (en) 1986-04-01
CA1286286C (en) 1991-07-16
ES8705894A1 (es) 1987-05-16
NO166187B (no) 1991-03-04
GR860677B (en) 1986-07-14
AU5472786A (en) 1986-09-18
ATE99702T1 (de) 1994-01-15
NZ215435A (en) 1989-01-06
ES552898A0 (es) 1987-05-16
NO166187C (no) 1991-06-12
FI861020A (fi) 1986-09-14
DE3689493D1 (de) 1994-02-17
DK113886A (da) 1986-09-14
KR910006809B1 (ko) 1991-09-02
EP0199033B1 (en) 1994-01-05
JPS61218601A (ja) 1986-09-29
ZA861855B (en) 1986-10-29
YU37586A (en) 1988-04-30
PH22370A (en) 1988-08-12
EP0199033A3 (en) 1988-03-30
YU46303B (sh) 1993-05-28
EP0199033A2 (en) 1986-10-29
DE3689493T2 (de) 1994-04-28
IT1208509B (it) 1989-07-10
PT82198B (pt) 1988-02-17
FI82700B (fi) 1990-12-31
AU582221B2 (en) 1989-03-16
NO860940L (no) 1986-09-15
FI861020A0 (fi) 1986-03-12
JPH0238601B2 (ko) 1990-08-31
FI82700C (fi) 1991-04-10
IT8519885A0 (it) 1985-03-13
DK113886D0 (da) 1986-03-12

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