KR860004147A - 에피더민의 제조방법 - Google Patents

에피더민의 제조방법 Download PDF

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KR860004147A
KR860004147A KR1019850008249A KR850008249A KR860004147A KR 860004147 A KR860004147 A KR 860004147A KR 1019850008249 A KR1019850008249 A KR 1019850008249A KR 850008249 A KR850008249 A KR 850008249A KR 860004147 A KR860004147 A KR 860004147A
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epidermin
methanol
butanol
acetic acid
chloroform
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KR1019850008249A
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KR930002736B1 (ko
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귄터 베르너(외4) 롤프
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베첼, 좀머
닥터 칼 토매 게젤샤프트 미트 베쉬랭크터 하프퉁
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Priority claimed from DE19843440423 external-priority patent/DE3440423A1/de
Priority claimed from DE19853523478 external-priority patent/DE3523478A1/de
Application filed by 베첼, 좀머, 닥터 칼 토매 게젤샤프트 미트 베쉬랭크터 하프퉁 filed Critical 베첼, 좀머
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Publication of KR930002736B1 publication Critical patent/KR930002736B1/ko

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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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    • A—HUMAN NECESSITIES
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/06—Preparations for care of the skin for countering cellulitis
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/44—Staphylococcus
    • C12R2001/45—Staphylococcus epidermidis

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Abstract

내용 없음

Description

에피더민의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 에피더민(Epidermin)의 완전한 산 가수분해물의 아미노산 크로마토그램이다(닌히드린 염색, λ＝570㎜).
제3도는 에피더민의 UV 스펙트럼이다.
제10도는 본 발명의 배지에 대하여 생존하는 박테리아의 수 및 마이크로코쿠스루테우스에 대한 활성을 비교한 그래프이다.

Claims (2)

  1. 스타필로코쿠스 에피더미디스(Staphylococcus epidermidis) DSM 3095를 2 내지 4%의 육즙 등의 질소원, 1 내지 3%의 당 또는 당알코올의 탄소원 및 0.25 내지 1%의 탄산염 및/또는 0.25 내지 0.5%의 알칼리토금속의 수산화물로 구성되는 복합 영양배지 용액중에서 34 내지 37℃의 호기 조건하에 성장시킨 후, 세포 및 무기염을 제거하고; 배양모액을 pH 8에서 n-부탄올로 추출하고, 부탄올 추출물을 증발시켜 그 잔류물을 메탄올에 용해시킨 다음 이 용액을 에테르 침전에 의해 지방성분을 제거하거나, 배양모액을 아크릴레이트 또는 폴리스티렌을 주재로 하는 중합체로 처리하고, 흡착된 에피더민을 메탄올/진한염산(99:1)에 의해 수지로 부터 유리시킨 다음 이 용액을 암모니아로 중화시키고, 진공중에서 증발 농축시켜, 생성된 활성물질을 단리시키고; 상기에서 단리한 물질을 겔크로마토그래피[메탄올/아세트산(95:5)를 사용하여 세파덱스 LH-20에서]하여 다른 저분자량 펩티드, 아미노산 및 염을 분리 제거하고; 일차적으로, n-부탄올/에틸아세테이트/0.1N 아세트산(3:1:3)의 시스템을 사용하여 액체-액체 분배를 수행하고, 연속하여, 이차적으로, 기선상에 남아있는 에피더민을 2-부탄올/0.05N암모늄 아세테이트(1:1) 중성 시스템을 사용하여 분배하는 크레이그의 다중 역류 분배를 수행하고; 용출물을 동결건조시켜 무색분말 형태의 순수한 에피더민을 단리시킴을 특징으로 하는, 하기의 일차구조를 가지며, 그 아미노산 조성이 Asn(1), Pro(1), Gly(2), Ala(2), He(2), Phe(2), Lys(2), Lan(2), β-Me-Lan(1), Dhb(1), Tyr(1) 및 S-(2-아미노비빌)-D-시스테인(1)인 항생물질 에피더민의 제조, 단리 및 정제방법.
    상기 구조식에서, N-말단에 인접한 개개의 티오에테르아미노산의 반이 D배치이다.
  2. 제1항에 있어서, 하기의 추가 특성을 갖는 에피더민의 제조방법.
    a. 형태:무색분말, b. 용해도:물/빙초산 또는 메탄올/빙초산의 혼합물에 매우 쉽게 용해; 저급 알코올에 용해; 클로로포름, 아세톤, 디에틸에테르, 석유 에테르에 불용. c. 실리카겔 플레이트 상에서 염색 반응;닌히드린, 염소/TDM[TDM＝4,4'＝비스-(디메틸아미노)-디페닐메탄], 오르신/술푸르산, 아니스알데히드/술푸르산. 254㎜의 UV에서, 물을 분무하여 비-파괴검출. d. 박층 크로마토그래피:실리카겔 플레이트 상품 60F254(Merck)를 사용하였다. 시스템 A:클로로포름/메탄올/17% 암모니아 (2/2/1) Rf＝0.73
    시스템 B:클로로포름/메탄올/17% 암모니아 시스템 C:n-부탄올/빙초산/물
    (70/35/10) Rf＝0.30 (4/1/1) Rf0.73
    e. HPLC:제7도 참조. f. 안정성 pH2 내지 pH7에서 안정. 그이상의 pH에서 활성이 급강하. g. 분자량:약 2160(음이온부재). h. U.V.흡수스펙트럼:수용액에서 λmax×267nm(제3도 참조). i. IR흡수스펙트럼:브롬화 칼륨 펠렛상 시료의 IR 스펙트럼(제4도). i. NMR 스펙트럼 1H-NMR 스펙트럼:제5도 13C-NMR 스펙트럼:제6도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019850008249A 1984-11-06 1985-11-05 에피더민의 제조방법 KR930002736B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DEP3440423.6 1984-11-06
DE19843440423 DE3440423A1 (de) 1984-11-06 1984-11-06 Antibiotisches polypeptid, verfahren zu seiner herstellung, dieses polypeptid erzeugender stamm von staphylococcus epidermidis, dieses polypeptid enthaltende zubereitungsformen und seine verwendung zur bekaempfung infektioeser erkrankungen
DEP3523478.4 1985-07-01
DE19853523478 DE3523478A1 (de) 1985-07-01 1985-07-01 Antibiotisches polypeptid, verfahren zu seiner herstellung, dieses polypeptid erzeugender stamm vom staphylococcus epidermidis, dieses polypeptid enthaltende zubereitungsformen und seine verwendung zur bekaempfung infektioeser erkrankungen

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KR860004147A true KR860004147A (ko) 1986-06-18
KR930002736B1 KR930002736B1 (ko) 1993-04-09

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EP (1) EP0181578B1 (ko)
JP (2) JPH0670078B2 (ko)
KR (1) KR930002736B1 (ko)
CA (1) CA1277617C (ko)
DE (1) DE3583987D1 (ko)
DK (1) DK172818B1 (ko)
ES (1) ES8702924A1 (ko)
FI (1) FI82073C (ko)
HK (1) HK63795A (ko)
IL (1) IL76956A (ko)
NO (1) NO164039C (ko)
NZ (1) NZ214067A (ko)
PT (1) PT81436B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023096177A1 (ko) * 2021-11-26 2023-06-01 코스맥스 주식회사 에피더미디박테리움 케라티니 추출물이 함침된 다공성 실리카 및 그의 피부상태 개선 용도

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0637384B2 (ja) * 1985-09-21 1994-05-18 株式会社資生堂 皮膚化粧料
DE58905744D1 (de) * 1988-07-15 1993-11-04 Thomae Gmbh Dr K Verfahren zur gewinnung, isolierung und reinigung von epidermin.
DE3938140A1 (de) * 1989-11-16 1991-08-08 Beiersdorf Ag Desodorierende kosmetische mittel
EP0659068B1 (en) * 1992-09-10 1996-12-04 Sara Lee/DE N.V. Mouth-care products with anti-bacterial activity
PT789581E (pt) * 1993-08-20 2001-11-30 Novartis Ag Metodo de prevencao e tratamento da mastite bovina
AU2408695A (en) * 1994-05-07 1995-11-29 Novartis Ag Lanthionine-containing antimicrobial compound
WO1996031192A1 (de) * 1995-04-07 1996-10-10 Kuyus-Stiftung Kosmetische zusammensetzung zur behandlung von cellulite
SE9602496D0 (sv) 1996-06-20 1996-06-20 Bengt Guss Method and means for producing a fibrinogen binding protein and its use in biotechnology
JP2006176406A (ja) * 2003-03-11 2006-07-06 Asahi Kasei Pharma Kk ペプチド系抗生物質

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023096177A1 (ko) * 2021-11-26 2023-06-01 코스맥스 주식회사 에피더미디박테리움 케라티니 추출물이 함침된 다공성 실리카 및 그의 피부상태 개선 용도

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DK172818B1 (da) 1999-08-02
NO164039B (no) 1990-05-14
JPS61158997A (ja) 1986-07-18
IL76956A0 (en) 1986-04-29
DK509985D0 (da) 1985-11-05
FI82073B (fi) 1990-09-28
EP0181578A2 (de) 1986-05-21
NZ214067A (en) 1991-05-28
FI854316A (fi) 1986-05-07
KR930002736B1 (ko) 1993-04-09
IL76956A (en) 1991-07-18
CA1277617C (en) 1990-12-11
EP0181578A3 (en) 1988-08-17
PT81436A (de) 1985-12-01
JPH0670078B2 (ja) 1994-09-07
PT81436B (pt) 1988-01-22
EP0181578B1 (de) 1991-09-04
DK509985A (da) 1986-05-07
NO854405L (no) 1986-05-07
DE3583987D1 (de) 1991-10-10
JPH0732704B2 (ja) 1995-04-12
FI854316A0 (fi) 1985-11-04
ES548544A0 (es) 1987-01-16
ES8702924A1 (es) 1987-01-16
HK63795A (en) 1995-05-05
FI82073C (fi) 1991-01-10
NO164039C (no) 1990-08-22
JPH06113826A (ja) 1994-04-26

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