KR860004147A - 에피더민의 제조방법 - Google Patents
에피더민의 제조방법 Download PDFInfo
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- KR860004147A KR860004147A KR1019850008249A KR850008249A KR860004147A KR 860004147 A KR860004147 A KR 860004147A KR 1019850008249 A KR1019850008249 A KR 1019850008249A KR 850008249 A KR850008249 A KR 850008249A KR 860004147 A KR860004147 A KR 860004147A
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- KR
- South Korea
- Prior art keywords
- epidermin
- methanol
- butanol
- acetic acid
- chloroform
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/06—Preparations for care of the skin for countering cellulitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/44—Staphylococcus
- C12R2001/45—Staphylococcus epidermidis
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Communicable Diseases (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Birds (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 에피더민(Epidermin)의 완전한 산 가수분해물의 아미노산 크로마토그램이다(닌히드린 염색, λ=570㎜).
제3도는 에피더민의 UV 스펙트럼이다.
제10도는 본 발명의 배지에 대하여 생존하는 박테리아의 수 및 마이크로코쿠스루테우스에 대한 활성을 비교한 그래프이다.
Claims (2)
- 스타필로코쿠스 에피더미디스(Staphylococcus epidermidis) DSM 3095를 2 내지 4%의 육즙 등의 질소원, 1 내지 3%의 당 또는 당알코올의 탄소원 및 0.25 내지 1%의 탄산염 및/또는 0.25 내지 0.5%의 알칼리토금속의 수산화물로 구성되는 복합 영양배지 용액중에서 34 내지 37℃의 호기 조건하에 성장시킨 후, 세포 및 무기염을 제거하고; 배양모액을 pH 8에서 n-부탄올로 추출하고, 부탄올 추출물을 증발시켜 그 잔류물을 메탄올에 용해시킨 다음 이 용액을 에테르 침전에 의해 지방성분을 제거하거나, 배양모액을 아크릴레이트 또는 폴리스티렌을 주재로 하는 중합체로 처리하고, 흡착된 에피더민을 메탄올/진한염산(99:1)에 의해 수지로 부터 유리시킨 다음 이 용액을 암모니아로 중화시키고, 진공중에서 증발 농축시켜, 생성된 활성물질을 단리시키고; 상기에서 단리한 물질을 겔크로마토그래피[메탄올/아세트산(95:5)를 사용하여 세파덱스 LH-20에서]하여 다른 저분자량 펩티드, 아미노산 및 염을 분리 제거하고; 일차적으로, n-부탄올/에틸아세테이트/0.1N 아세트산(3:1:3)의 시스템을 사용하여 액체-액체 분배를 수행하고, 연속하여, 이차적으로, 기선상에 남아있는 에피더민을 2-부탄올/0.05N암모늄 아세테이트(1:1) 중성 시스템을 사용하여 분배하는 크레이그의 다중 역류 분배를 수행하고; 용출물을 동결건조시켜 무색분말 형태의 순수한 에피더민을 단리시킴을 특징으로 하는, 하기의 일차구조를 가지며, 그 아미노산 조성이 Asn(1), Pro(1), Gly(2), Ala(2), He(2), Phe(2), Lys(2), Lan(2), β-Me-Lan(1), Dhb(1), Tyr(1) 및 S-(2-아미노비빌)-D-시스테인(1)인 항생물질 에피더민의 제조, 단리 및 정제방법.상기 구조식에서, N-말단에 인접한 개개의 티오에테르아미노산의 반이 D배치이다.
- 제1항에 있어서, 하기의 추가 특성을 갖는 에피더민의 제조방법.a. 형태:무색분말, b. 용해도:물/빙초산 또는 메탄올/빙초산의 혼합물에 매우 쉽게 용해; 저급 알코올에 용해; 클로로포름, 아세톤, 디에틸에테르, 석유 에테르에 불용. c. 실리카겔 플레이트 상에서 염색 반응;닌히드린, 염소/TDM[TDM=4,4'=비스-(디메틸아미노)-디페닐메탄], 오르신/술푸르산, 아니스알데히드/술푸르산. 254㎜의 UV에서, 물을 분무하여 비-파괴검출. d. 박층 크로마토그래피:실리카겔 플레이트 상품 60F254(Merck)를 사용하였다. 시스템 A:클로로포름/메탄올/17% 암모니아 (2/2/1) Rf=0.73시스템 B:클로로포름/메탄올/17% 암모니아 시스템 C:n-부탄올/빙초산/물(70/35/10) Rf=0.30 (4/1/1) Rf0.73e. HPLC:제7도 참조. f. 안정성 pH2 내지 pH7에서 안정. 그이상의 pH에서 활성이 급강하. g. 분자량:약 2160(음이온부재). h. U.V.흡수스펙트럼:수용액에서 λmax×267nm(제3도 참조). i. IR흡수스펙트럼:브롬화 칼륨 펠렛상 시료의 IR 스펙트럼(제4도). i. NMR 스펙트럼 1H-NMR 스펙트럼:제5도 13C-NMR 스펙트럼:제6도.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP3440423.6 | 1984-11-06 | ||
DE19843440423 DE3440423A1 (de) | 1984-11-06 | 1984-11-06 | Antibiotisches polypeptid, verfahren zu seiner herstellung, dieses polypeptid erzeugender stamm von staphylococcus epidermidis, dieses polypeptid enthaltende zubereitungsformen und seine verwendung zur bekaempfung infektioeser erkrankungen |
DEP3523478.4 | 1985-07-01 | ||
DE19853523478 DE3523478A1 (de) | 1985-07-01 | 1985-07-01 | Antibiotisches polypeptid, verfahren zu seiner herstellung, dieses polypeptid erzeugender stamm vom staphylococcus epidermidis, dieses polypeptid enthaltende zubereitungsformen und seine verwendung zur bekaempfung infektioeser erkrankungen |
Publications (2)
Publication Number | Publication Date |
---|---|
KR860004147A true KR860004147A (ko) | 1986-06-18 |
KR930002736B1 KR930002736B1 (ko) | 1993-04-09 |
Family
ID=25826263
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019850008249A KR930002736B1 (ko) | 1984-11-06 | 1985-11-05 | 에피더민의 제조방법 |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0181578B1 (ko) |
JP (2) | JPH0670078B2 (ko) |
KR (1) | KR930002736B1 (ko) |
CA (1) | CA1277617C (ko) |
DE (1) | DE3583987D1 (ko) |
DK (1) | DK172818B1 (ko) |
ES (1) | ES8702924A1 (ko) |
FI (1) | FI82073C (ko) |
HK (1) | HK63795A (ko) |
IL (1) | IL76956A (ko) |
NO (1) | NO164039C (ko) |
NZ (1) | NZ214067A (ko) |
PT (1) | PT81436B (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023096177A1 (ko) * | 2021-11-26 | 2023-06-01 | 코스맥스 주식회사 | 에피더미디박테리움 케라티니 추출물이 함침된 다공성 실리카 및 그의 피부상태 개선 용도 |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0637384B2 (ja) * | 1985-09-21 | 1994-05-18 | 株式会社資生堂 | 皮膚化粧料 |
DE58905744D1 (de) * | 1988-07-15 | 1993-11-04 | Thomae Gmbh Dr K | Verfahren zur gewinnung, isolierung und reinigung von epidermin. |
DE3938140A1 (de) * | 1989-11-16 | 1991-08-08 | Beiersdorf Ag | Desodorierende kosmetische mittel |
EP0659068B1 (en) * | 1992-09-10 | 1996-12-04 | Sara Lee/DE N.V. | Mouth-care products with anti-bacterial activity |
PT789581E (pt) * | 1993-08-20 | 2001-11-30 | Novartis Ag | Metodo de prevencao e tratamento da mastite bovina |
AU2408695A (en) * | 1994-05-07 | 1995-11-29 | Novartis Ag | Lanthionine-containing antimicrobial compound |
WO1996031192A1 (de) * | 1995-04-07 | 1996-10-10 | Kuyus-Stiftung | Kosmetische zusammensetzung zur behandlung von cellulite |
SE9602496D0 (sv) | 1996-06-20 | 1996-06-20 | Bengt Guss | Method and means for producing a fibrinogen binding protein and its use in biotechnology |
JP2006176406A (ja) * | 2003-03-11 | 2006-07-06 | Asahi Kasei Pharma Kk | ペプチド系抗生物質 |
-
1985
- 1985-10-31 DE DE8585113908T patent/DE3583987D1/de not_active Expired - Lifetime
- 1985-10-31 EP EP85113908A patent/EP0181578B1/de not_active Expired - Lifetime
- 1985-11-04 FI FI854316A patent/FI82073C/fi not_active IP Right Cessation
- 1985-11-05 PT PT81436A patent/PT81436B/pt unknown
- 1985-11-05 CA CA000494652A patent/CA1277617C/en not_active Expired - Lifetime
- 1985-11-05 DK DK198505099A patent/DK172818B1/da not_active IP Right Cessation
- 1985-11-05 KR KR1019850008249A patent/KR930002736B1/ko not_active IP Right Cessation
- 1985-11-05 IL IL76956A patent/IL76956A/xx not_active IP Right Cessation
- 1985-11-05 ES ES548544A patent/ES8702924A1/es not_active Expired
- 1985-11-05 NZ NZ214067A patent/NZ214067A/en unknown
- 1985-11-05 NO NO854405A patent/NO164039C/no not_active IP Right Cessation
- 1985-11-05 JP JP60247876A patent/JPH0670078B2/ja not_active Expired - Lifetime
-
1993
- 1993-07-13 JP JP5173284A patent/JPH0732704B2/ja not_active Expired - Lifetime
-
1995
- 1995-04-27 HK HK63795A patent/HK63795A/xx not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023096177A1 (ko) * | 2021-11-26 | 2023-06-01 | 코스맥스 주식회사 | 에피더미디박테리움 케라티니 추출물이 함침된 다공성 실리카 및 그의 피부상태 개선 용도 |
Also Published As
Publication number | Publication date |
---|---|
DK172818B1 (da) | 1999-08-02 |
NO164039B (no) | 1990-05-14 |
JPS61158997A (ja) | 1986-07-18 |
IL76956A0 (en) | 1986-04-29 |
DK509985D0 (da) | 1985-11-05 |
FI82073B (fi) | 1990-09-28 |
EP0181578A2 (de) | 1986-05-21 |
NZ214067A (en) | 1991-05-28 |
FI854316A (fi) | 1986-05-07 |
KR930002736B1 (ko) | 1993-04-09 |
IL76956A (en) | 1991-07-18 |
CA1277617C (en) | 1990-12-11 |
EP0181578A3 (en) | 1988-08-17 |
PT81436A (de) | 1985-12-01 |
JPH0670078B2 (ja) | 1994-09-07 |
PT81436B (pt) | 1988-01-22 |
EP0181578B1 (de) | 1991-09-04 |
DK509985A (da) | 1986-05-07 |
NO854405L (no) | 1986-05-07 |
DE3583987D1 (de) | 1991-10-10 |
JPH0732704B2 (ja) | 1995-04-12 |
FI854316A0 (fi) | 1985-11-04 |
ES548544A0 (es) | 1987-01-16 |
ES8702924A1 (es) | 1987-01-16 |
HK63795A (en) | 1995-05-05 |
FI82073C (fi) | 1991-01-10 |
NO164039C (no) | 1990-08-22 |
JPH06113826A (ja) | 1994-04-26 |
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