KR20240038564A - High content collagen food composition with antioxidant, skin inflammation improvement, and skin elasticity increase effect - Google Patents
High content collagen food composition with antioxidant, skin inflammation improvement, and skin elasticity increase effect Download PDFInfo
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- KR20240038564A KR20240038564A KR1020230052076A KR20230052076A KR20240038564A KR 20240038564 A KR20240038564 A KR 20240038564A KR 1020230052076 A KR1020230052076 A KR 1020230052076A KR 20230052076 A KR20230052076 A KR 20230052076A KR 20240038564 A KR20240038564 A KR 20240038564A
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Abstract
본 발명은 항산화, 피부염증개선 및 피부탄력 증가 효과를 갖는 고함량 콜라겐 식품 조성물에 관한 것으로서, 상세하게는 저분자 피쉬 콜라겐에 글리신, L-글루타민, L-시스테인, L-라이신, 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎 추출물을 혼합하여 항산화, 피부염증 개선, 피부 탄력, 피부 주름 등의 피부 개선 효과를 가지는 식품 및 이의 제조방법에 관한 것이다. The present invention relates to a high-content collagen food composition with anti-oxidation, skin inflammation improvement, and skin elasticity-increasing effects, specifically, low-molecular-weight fish collagen, glycine, L-glutamine, L-cysteine, L-lysine, and Dracocephalum moldavia. The present invention relates to a food product that has skin improvement effects such as antioxidant, skin inflammation improvement, skin elasticity, and skin wrinkles by mixing Moldavian dragonhead leaf extract, and a method of manufacturing the same.
Description
본 발명은 항산화, 피부염증개선 및 피부탄력 증가 효과를 갖는 고함량 콜라겐 식품 조성물에 관한 것으로서, 상세하게는 저분자 피쉬 콜라겐에 글리신, L-글루타민, L-시스테인, L-라이신, 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎 추출물을 혼합하여 항산화, 피부염증 개선, 피부 탄력, 피부 주름 등의 피부 개선 효과를 가지는 식품 및 이의 제조방법에 관한 것이다. The present invention relates to a high-content collagen food composition with anti-oxidation, skin inflammation improvement, and skin elasticity-increasing effects, specifically, low-molecular-weight fish collagen, glycine, L-glutamine, L-cysteine, L-lysine, and Dracocephalum moldavia. The present invention relates to a food product that has skin improvement effects such as antioxidant, skin inflammation improvement, skin elasticity, and skin wrinkles by mixing Moldavian dragonhead leaf extract, and a method of manufacturing the same.
콜라겐(Collagen)이란 신체 구성 성분 중 가장 많이 차지하고 있는 단백질 중 하나로서, 동물의 뼈, 연골, 피부 외에 물고기의 비늘 등을 구성한다. 콜라겐은 섬유상 고체로 존재하고, 물, 묽은 산, 묽은 알칼리에 녹지 않지만 끓이면 젤라틴이 되어 용해되며, 콜라게나아제(Collagenase)의 작용을 받는다. 콜라겐을 구성하는 아미노산으로는 프롤린, 옥시프롤린, 글리신, 글루탐산 등이 있으며, 그 중에서도 다른 단백질에 존재하지 않는 옥시프롤린의 함량이 높은 것이 특징이다. Collagen is one of the most common proteins in the body and makes up the bones, cartilage, and skin of animals, as well as the scales of fish. Collagen exists as a fibrous solid and is insoluble in water, dilute acid, or dilute alkali, but when boiled, it becomes gelatin and dissolves, and is subject to the action of collagenase. The amino acids that make up collagen include proline, oxyproline, glycine, and glutamic acid, and it is characterized by a high content of oxyproline, which does not exist in other proteins.
인체의 콜라겐은 피부, 근육, 혈관, 장기, 눈, 치아, 세포, 뼈, 관절, 연골의 주성분이며, 망상 조직, 세포막, 머리카락, 태반 등의 주성분으로 족발, 사골국물, 닭발, 돼지껍데기, 도가니탕, 어류나 그 껍질 등에 많이 포함되어 있다. 콜라겐은 20대가 지나면서 점차 감소하기 시작하여 40대에 들어가면 그 절반수준으로 감소하는데 특히 여성들은 폐경 후 5년 사이에 30%가 감소된다. 이는 여성호르몬인 에스트로겐 분비가 떨어져 콜라겐의 합성을 돕지 못하기 때문이다. Collagen in the human body is the main component of skin, muscles, blood vessels, organs, eyes, teeth, cells, bones, joints, and cartilage. It is also the main component of reticular tissue, cell membranes, hair, and placenta, and is found in pig's feet, bone broth, chicken feet, pork skin, and dogani soup. , It is contained in large quantities in fish and their skins. Collagen gradually begins to decrease after one's 20s, and decreases to half by the time one enters one's 40s. In particular, women experience a 30% decrease in the five years after menopause. This is because the secretion of estrogen, a female hormone, decreases and does not help the synthesis of collagen.
콜라겐이 부족하면 피부탄력, 혈관탄력이 떨어지고, 퇴행성관절염이나 각종 만성질환을 유발할 수 있으며, 머리카락이 가늘어지고 탈모의 요인이 된다. 동물, 어류, 식물로부터 콜라겐을 추출하여 경구로 섭취시 피부의 주름개선, 보습증진, 탄력증가에 유효하며, 모발과 손톱, 발톱의 강화에 도움이 된다는 연구가 보고되면서 콜라겐을 섭취할 수 있는 식품 또는 화장품 등의 개발이 이루어지고 있다.A lack of collagen reduces skin elasticity and blood vessel elasticity, can cause degenerative arthritis and various chronic diseases, and can cause thinning hair and hair loss. Collagen is extracted from animals, fish, and plants and when consumed orally, it is effective in improving wrinkles, improving moisture, and increasing elasticity of the skin. As studies have shown that it helps strengthen hair, fingernails, and toenails, collagen is a food that can be consumed. Or cosmetics, etc. are being developed.
동물 유래 콜라겐은 돼지껍질, 족발, 닭발, 닭날개, 달걀흰자 등에서 주로 추출하며, 분자가 큰 동물 유래 콜라겐으로서 함량이 2%에 불과하다. 어류 유래 콜라겐은 피쉬 콜라겐(fish collagen)이라고도 불리며, 주로 북어껍질, 장어, 상어, 꽁치, 가자미, 새우, 자라, 홍어, 연어, 해삼, 소라 등으로부터 추출한다. 어피(fish skin)나 어린(fish scale)으로부터 얻이진 콜라겐은 인체의 구조와 흡사하여 그 흡수율이 84%에 달하고 시중에서 쉽게 구할 수 있으며, 동물 유래 콜라겐에 비해 분자량이 작아 인체 흡수량을 상대적으로 높일 수 있는 장점을 가진다. 그럼에도 불구하고 콜라겐의 분자량 자체가 커서, 체내에서 소장으로 흡수되어 피부까지 도달할 수 있는 흡수율이 현저히 낮아, 분자량을 1,000~5,000Da 정도까지 저분자화시킨 피쉬 콜라겐이 개발되고 있다.Animal-derived collagen is mainly extracted from pig skin, pig's feet, chicken feet, chicken wings, and egg whites. It is a large molecule animal-derived collagen and has a content of only 2%. Fish-derived collagen is also called fish collagen, and is mainly extracted from pollack skin, eel, shark, saury, flounder, shrimp, turtle, skate, salmon, sea cucumber, and conch. Collagen obtained from fish skin or fish scale is similar to the structure of the human body, has an absorption rate of 84%, and is easily available on the market. It has a smaller molecular weight than animal-derived collagen, which relatively increases the amount of absorption into the human body. It has the advantage of being able to Nevertheless, because the molecular weight of collagen itself is large, the absorption rate at which it can be absorbed from the body into the small intestine and reach the skin is significantly low, so fish collagen with a molecular weight reduced to about 1,000 to 5,000 Da is being developed.
피쉬 콜라겐의 분자량을 낮추기 위한 방법으로 기존에는 어류 껍질을 원료로 산 또는 가열 등의 처리 공정으로 연화시키고, 단백질 가수분해 효소로 분해한 후 여과하는 방법이 사용되고 있다. 그러나 상기와 같은 피쉬 콜라겐은 분자랑 5kDA 초과하는 크기의 펩티드를 다량 포함하여 콜라겐 입자의 크기가 커서 피부에 도입하기에 적합할 정도로 저분화되지 않고, 미세한 이취가 잔존하는 문제가 있다. As a method to lower the molecular weight of fish collagen, a method has been used that uses fish skin as a raw material, softens it through a treatment process such as acid or heating, decomposes it with a proteolytic enzyme, and then filters it. However, fish collagen as described above contains a large amount of peptides with a molecular size exceeding 5 kDA, so the size of the collagen particles is large, so it is not low-differentiated enough to be suitable for introduction into the skin, and there is a problem that a subtle off-flavor remains.
피쉬 콜라겐의 이취를 제거하기 위해 고순도 정제를 진행하기도 하나, 고순도 정제를 위한 공정 단계로 인하여 콜라겐 소재의 단가가 상승하는 단점이 있기에 이를 대체하여 어류의 비린취를 저감시킬 수 있는 마스킹(masking) 소재를 처리하는 방법에 대한 개발이 활발히 이루어지고 있다. In order to remove the off-flavor of fish collagen, high-purity purification is performed, but the process step for high-purity purification has the disadvantage of increasing the unit price of the collagen material, so it can be replaced with a masking material that can reduce the fishy odor of fish. Development of methods for processing is actively underway.
한국공개특허 제10-2013-0098729호는 어류 유래 저분자 콜라겐 펩티드 제조방법을 개시하고 있으며, 특히 어류에서 나온 어린을 전처리한 후 가수분해하여 여과, 정제 공정을 거쳐 제조된 피쉬 콜라겐 펩티드(fish collagen peptide)의 제조방법을 개시한다. 기존 동물 유래 콜라겐에 비해 저분자 분포가 높고, 콜라겐의 주요 지표성분인 하이드록시 프롤린(hydroxyl proline)의 함량이 증진된 것을 특징으로 하며, 제품에 적용이 용이하도록 색도 및 비린취 성분이 크게 저감된 피쉬 콜라겐 펩티드의 제조방법을 개시한다. Korean Patent Publication No. 10-2013-0098729 discloses a method for producing low-molecular-weight collagen peptides derived from fish, and in particular, fish collagen peptides prepared by pre-treating young fish, followed by hydrolysis, filtration, and purification processes. ) manufacturing method is disclosed. Compared to existing animal-derived collagen, it has a higher distribution of low molecules and an increased content of hydroxyl proline, a major indicator of collagen. Fish color and fishy odor components have been greatly reduced for easy application to products. Disclosed is a method for producing collagen peptides.
체내 흡수율을 높이는 방법 외에도, 콜라겐을 함유한 식품 또는 화장품의 기능성을 향상시키기 위한 연구도 진행되고 있으며, 한국공개특허 제10-2018-0052980호에서는 산자나무 종자 오일 및 콜라겐을 포함하는 복합 분말 및 이를 포함하는 화장료 조성물을 개시한다. 산자나무 종자에 함유된 다양한 필수 지방산, 비타민, 카로티노이드 등의 성분이 자외선을 흡수하여 피부건강 증진 효과를 가진다.In addition to ways to increase the absorption rate in the body, research is also being conducted to improve the functionality of foods or cosmetics containing collagen, and in Korea Patent Publication No. 10-2018-0052980, there is a composite powder containing sea buckthorn seed oil and collagen and the same. Disclosed is a cosmetic composition comprising: The various essential fatty acids, vitamins, and carotenoids contained in sea buckthorn seeds absorb ultraviolet rays and have the effect of improving skin health.
건강과 미용에 대한 관심이 지속적으로 증가하는 가운데, 콜라겐을 적용한 식품, 화장품을 찾는 사람들은 지속적으로 증가하고 있어, 이에 따라 효능을 높이기 위해 체내 흡수율을 높일 수 있는 공정 기술, 기능성을 높이기 위한 기술, 소비자들의 기호성을 높일 수 있는 연구에 필요성은 증가하고 있다.As interest in health and beauty continues to increase, the number of people looking for collagen-based foods and cosmetics continues to increase. Accordingly, processing technology that can increase absorption in the body to increase efficacy, technology to increase functionality, The need for research that can increase consumer preference is increasing.
본 발명의 목적은 피쉬 콜라겐, 아미노산 4종(글리신, L-글루타민, L-시스테인, L-라이신) 및 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎 추출물을 포함하는 식품 조성물에 관한 것으로, 상기 조성물을 통해 피부 개선 효과를 높이고, 저분자 피쉬 콜라겐의 이취를 최소화하여 소비자의 기호도를 높인 식품 조성물 및 이의 제조방법을 제공하는 것이다. The object of the present invention relates to a food composition containing fish collagen, four amino acids (glycine, L-glutamine, L-cysteine, L-lysine) and Moldavian dragonhead leaf extract, the composition The aim is to provide a food composition and a manufacturing method thereof that enhance the skin improvement effect and minimize the off-flavor of low-molecular-weight fish collagen, thus increasing consumer preference.
본 발명의 일 측면은 분자량 500 내지 3,000 Da의 분자량을 가지는 피쉬 콜라겐, 복합 아미노산 및 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎 추출물을 포함하는 피부 상태 개선용 식품 조성물로서, 상기 복합 아미노산은 글리신(glycine), L-글루타민(L-glutamine), L-시스테인(L-cysteine) 및 L-라이신(L-lysine)을 포함하는 것인, 피부 상태 개선용 식품 조성물에 관한 것이다. One aspect of the present invention is a food composition for improving skin condition comprising fish collagen having a molecular weight of 500 to 3,000 Da, a complex amino acid, and a Moldavian dragonhead leaf extract, wherein the complex amino acid is glycine ( It relates to a food composition for improving skin condition, comprising glycine, L-glutamine, L-cysteine, and L-lysine.
상기 콜라겐은 세포와 세포 사이에 접착제의 역할을 하고, 피부 탄력 유지, 노화 방지에 효능이 있는 성분으로, 인체를 구성하는 단백질 중 30 내지 40% 비율로 포함되며, 장기, 관절, 연골, 눈의 각막, 뼈와 피부 등에 주로 분포하고, 특히 칼슘 접착 기능과 주름의 원인이 되는 진피층의 구성요소이다.The collagen acts as an adhesive between cells and is an ingredient that is effective in maintaining skin elasticity and preventing aging. It is contained in a ratio of 30 to 40% of the proteins that make up the human body, and is a vital component of organs, joints, cartilage, and eyes. It is mainly distributed in the cornea, bones and skin, and is a component of the dermal layer, which has a calcium adhesion function and is the cause of wrinkles.
상기 콜라겐은 돼지 등의 동물 유래 콜라겐 또는 어류 유래 콜라겐인 피쉬 콜라겐(fish collagen)을 사용할 수 있으며, 바람직하게는 동물 유래 콜라겐에 비해 분자량이 작고, 하이드록시 프롤린(hydroxyl proline)의 함량이 높은 피쉬 콜라겐일 수 있다. The collagen may be animal-derived collagen such as pigs or fish collagen, which is fish-derived collagen. Preferably, fish collagen has a lower molecular weight and a higher hydroxyl proline content than animal-derived collagen. It can be.
상기 피쉬 콜라겐은 일반적인 길이가 대략 300 nm 이상으로, 동물 유래 콜라겐보다 부피가 작더라도 피부 도입 또는 체내 흡수가 쉽지 않다. 이에 본 발명에서는 피쉬 콜라겐의 저분자화 공정을 통해 500 내지 3,000 Da의 분자량을 가진 저분자 피쉬 콜라겐을 이용할 수 있다.The fish collagen has a general length of approximately 300 nm or more, and although it is smaller in volume than animal-derived collagen, it is not easy to be introduced into the skin or absorbed into the body. Accordingly, in the present invention, low-molecular-weight fish collagen with a molecular weight of 500 to 3,000 Da can be used through a low-molecular-weight process of fish collagen.
상기 저분자 피쉬 콜라겐은 하이드록시 프롤린 등과 같은 아미노산의 체내 흡수되는 양이 증가하여 진피층의 섬유아세포에서 프로콜라겐의 합성을 증진시키고, 체내 콜라겐의 합성을 촉진시켜 피부 탄력을 증진시키는 역할을 할 수 있다. The low-molecular-weight fish collagen increases the amount of amino acids such as hydroxyproline absorbed into the body, thereby enhancing the synthesis of procollagen in the fibroblasts of the dermal layer and promoting the synthesis of collagen in the body, thereby improving skin elasticity.
상기 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎은 꿀풀과(Lamiaceae) 용머리속(Dracocephalum)에 속하는 초본으로, 중국, 러시아, 미국 등에 자생 또는 분포되어 있다. The Dracocephalum Moldavica ( Moldavian dragonhead ) leaf is an herb belonging to the Lamiaceae genus Dracocephalum and is native or distributed in China, Russia, the United States, etc.
상기 드라코세팔룸 몰다비카는 중앙아시아에서 관상동맥, 심장질환, 심폐기능 치료에 사용되어왔으며, 여러 플라보노이드 성분을 풍부하게 함유하고 있어 항산화, 노화방지에 효능이 있다고 알려져 있다. The Dracocephalum moldavica has been used in Central Asia to treat coronary artery disease, heart disease, and cardiopulmonary function, and is known to be effective in antioxidants and anti-aging as it is rich in various flavonoid ingredients.
상기 아미노산은 신체를 구성하는 단백질 조직의 주요 성분으로, 구조에 따라 20여가지로 분리된다. 신체의 조직 중 가장 큰 부분을 차지하는 피부 조직 중 각질층도 일정 성분비율의 아미노산을 가지고 있으며 각질층의 보호 또는 활성화되어 피부보습 및 탄력있는 피부가 유지되기 위해서는 각질층이 일정량의 독립된 아미노산을 함유하여야 한다. 이러한 작용을 나타내기 위하여 본 발명에서는 복합 아미노산을 포함하여 피부 각질층의 아미노산 비율로 복합물을 제조하여 피부 개선 효과를 제공하고자 한다. The amino acids are the main components of the protein tissue that makes up the body, and are divided into about 20 types depending on their structure. Among the skin tissues that make up the largest part of the body's tissues, the stratum corneum also has a certain percentage of amino acids. In order to protect or activate the stratum corneum to maintain skin moisture and elasticity, the stratum corneum must contain a certain amount of independent amino acids. In order to exhibit this effect, the present invention seeks to provide a skin improvement effect by preparing a complex containing complex amino acids and the amino acid ratio of the stratum corneum of the skin.
상기 글리신은 체내에서 합성할 수 있는 비필수 아미노산의 일종으로 불면증을 개선하는 효과를 가진다. 상기 글리신은 콜라겐을 구성하는 아미노산으로 피부의 탄력을 유지하는 역할을 하며 신경전달 물질로써의 역할 등 신체에서 여러 중요한 기능에 기여하고 있는 성분 중 하나이다. The glycine is a type of non-essential amino acid that can be synthesized in the body and has the effect of improving insomnia. The glycine is an amino acid that makes up collagen, plays a role in maintaining skin elasticity, and is one of the ingredients that contributes to various important functions in the body, such as serving as a neurotransmitter.
상기 L-글루타민은 2-글루타람산을 의미하며, 준-필수 아미노산, 비-대전된 극성 및 친수성 아미노산 중 하나로서, 다수 문헌을 통해 약리학적 특성들이 개시되어 있다. 상처 치유 또는 회복을 위한 경구 보충제, 정맥 영양제로 활용되고 있다. 또한 각질 세포의 이동에 기초하여 피부 재생에 기여하며 피부에 대한 생물학적 활성을 가진다. The L-glutamine refers to 2-glutaric acid, and is one of the semi-essential amino acids, non-charged polar and hydrophilic amino acids, and its pharmacological properties have been disclosed in numerous literature. It is used as an oral supplement or intravenous nutritional agent for wound healing or recovery. It also contributes to skin regeneration based on the movement of keratinocytes and has biological activity on the skin.
상기 L-시스테인은 아미노산의 한 종류로 글루타치온 생성을 도와 피부 미백 및 피부 재셍에 효과를 가지는 성분 중 하나이다. 글루타치온은 주로 간과 피부세포에 분포하여 멜라닌 색소의 생성을 억제해 피부 미백과 피부 재생에 도움을 주는 성분이다. The L-cysteine is a type of amino acid and is one of the ingredients that helps produce glutathione and is effective in skin whitening and skin rejuvenation. Glutathione is an ingredient that is mainly distributed in the liver and skin cells and helps with skin whitening and skin regeneration by suppressing the production of melanin pigment.
상기 L-라이신은 필수 아미노산 중 하나로서, 지방산을 에너지로 변환하고, 콜레스테롤 수치를 낮추는 카르니틴 생성에 중요한 역할을 한다. 피부, 힘줄, 뼈 등 연결 조직을 구성하는 구조 단백질 콜라겐과 엘라스틴 유지에 도움을 주는 아미노산 중 하나이다. The L-lysine is one of the essential amino acids and plays an important role in converting fatty acids into energy and producing carnitine, which lowers cholesterol levels. It is one of the amino acids that helps maintain collagen and elastin, the structural proteins that make up connective tissues such as skin, tendons, and bones.
상기 식품 조성물의 함량은 피쉬 콜라겐 85~90 중량부, 복합 아미노산 8~15 중량부, 드라코세팔룸 몰다비카 잎 추출물 0.01~0.2 중량부이며, 상기 복합 아미노산은 글리신, L-글루타민, L-시스테인 및 L-라이신을 8~12 : 3~6 : 2~4 : 0.5~2의 비율로 혼합된 것일 수 있다. 구체적으로 글리신, L-글루타민, L-시스테인 및 L-라이신은 10 : 4.9 : 2.5 : 1인 것이 바람직할 수 있다. The content of the food composition is 85 to 90 parts by weight of fish collagen, 8 to 15 parts by weight of complex amino acids, and 0.01 to 0.2 parts by weight of Dracocephalum moldavica leaf extract, and the complex amino acids are glycine, L-glutamine, and L-cysteine. and L-lysine may be mixed in a ratio of 8-12:3-6:2-4:0.5-2. Specifically, the ratio of glycine, L-glutamine, L-cysteine, and L-lysine may be 10:4.9:2.5:1.
상기 식품 조성물은 모과분말 0.01~0.05 중량부 및 레몬분말 0.01~0.05 중량부로 이루어진 군에서 선택된 어느 하나 이상을 추가로 포함할 수 있다. The food composition may further include at least one selected from the group consisting of 0.01 to 0.05 parts by weight of quince powder and 0.01 to 0.05 parts by weight of lemon powder.
상기 피부 상태 개선은 피부 재생, 피부 보습, 피부 탄력 개선, 피부 주름 개선, 피부 염증 개선 또는 외부 자극에 의한 손상 피부 장벽 개선인 것을 특징으로 한다. The improvement of the skin condition is characterized by skin regeneration, skin moisturization, improvement of skin elasticity, improvement of skin wrinkles, improvement of skin inflammation, or improvement of skin barrier damaged by external stimuli.
상기 피부 재생 및 피부 탄력은 진피 내의 콜라겐의 함량과 연관성이 있다. MMP(Matrix metalloproteinase)는 콜라겐 분해 효소로서, MMP의 활성이 증가하면 콜라겐의 분해가 촉진된다. MMP의 발현이 증가하면 콜라겐의 분해를 증가시키며, 진피층의 구조를 손상시켜 궁극적으로 피부의 주름(노화)을 유발시키게 된다. The skin regeneration and skin elasticity are related to the content of collagen in the dermis. MMP (Matrix metalloproteinase) is a collagen-degrading enzyme, and when MMP activity increases, collagen decomposition is promoted. When MMP expression increases, collagen decomposition increases and the structure of the dermal layer is damaged, ultimately causing wrinkles (aging) of the skin.
본 발명의 일 실시예에서는 세포 수준에서 MMP-1 저해활성을 측정한 결과, 본 발명의 식품 조성물이 MMP-1 억제 효과가 높게 나타나며, 콜라겐 발현을 유도함으로써 피부 탄력, 피부 주름 개선에 효과가 있음을 확인하였다(표 4 및 표 5). In one embodiment of the present invention, as a result of measuring MMP-1 inhibitory activity at the cellular level, the food composition of the present invention showed a high MMP-1 inhibitory effect and was effective in improving skin elasticity and skin wrinkles by inducing collagen expression. was confirmed (Table 4 and Table 5).
본 발명에서 “염증개선(완화)” 효과라 함은 염증을 억제하는 것을 말하며, 염증은 자극에 대한 생체조직의 방어반응의 하나로 조직 변질, 순환 장애와 삼출, 조직 증식의 세가지를 병발하는 복잡한 병변을 말한다. 염증반응에서는 초기단계 면역반응을 맡고 있는 백혈구들이 몰려들어 사이토카인을 발현한다. 따라서 세포내 사이토카인의 발현양이 염증반응 활성화의 지표가될 수 있다. 상기 염증반응 활성화와 관련되는 사이토카인은 IL-1a, IL-6, IL-12 등이 있다.In the present invention, the “inflammation improvement (relief)” effect refers to suppressing inflammation, and inflammation is one of the defense responses of living tissue to stimulation and is a complex lesion that causes three things: tissue deterioration, circulatory failure and exudation, and tissue proliferation. says In an inflammatory response, white blood cells responsible for the initial immune response flock in and express cytokines. Therefore, the expression level of intracellular cytokines can be an indicator of inflammatory response activation. Cytokines associated with the activation of the inflammatory response include IL-1a, IL-6, and IL-12.
상기 염증과 관련한 피부질환은 아토피 피부염, 접촉성 피부염, 지루성 피부염, 건선, 알레르기에 의한 가려움증 등이 있으며, 이에 한정되는 것은 아니다. 상기 염증과 관련된 피부질환의 증상으로는 홍반, 구진 수포, 과도한 각질형성, 가려움증 등이 있으나 이에 한정되는 것은 아니다. Skin diseases related to the above inflammation include, but are not limited to, atopic dermatitis, contact dermatitis, seborrheic dermatitis, psoriasis, and itching due to allergies. Symptoms of skin diseases related to the above inflammation include, but are not limited to, erythema, papules, blisters, excessive keratinization, and itching.
상기 피부 상태 개선용 식품 조성물은 아토피 피부염 개선 용도를 추가로 더 포함할 수 있다. The food composition for improving skin condition may further include a use for improving atopic dermatitis.
본 발명의 일 실시예에서 진피에 피부자극물질을 주어 피부염증이 일어난 상태와 유사한 환경을 만들어준 후 본 발명의 식품 조성물을 처리한 결과, IL-1a의 방출량이 현저히 감소됨을 확인하여, 피부염증 또는 외부 자극에 의한 손상 피부 장벽 개선에 효과가 있음을 확인하였다(표 6). In one embodiment of the present invention, it was confirmed that the amount of IL-1a released was significantly reduced as a result of treating the food composition of the present invention after creating an environment similar to the state in which skin inflammation occurred by applying a skin irritant to the dermis, thereby reducing skin inflammation. Additionally, it was confirmed that it was effective in improving the skin barrier damaged by external stimuli (Table 6).
본 발명의 다른 측면은 (a) 피쉬 콜라겐에 녹차 추출물을 1 : 0.01 내지 1 : 0.1 중량비로 혼합하는 단계; 및 (c) 상기 피쉬 콜라겐에 글리신, L-글루타민, L-시스테인 및 L-라이신을 8~12 : 3~6 : 2~4 : 0.5~2의 비율로 혼합한 복합 아미노산 및 드라코세팔룸 몰다비카 잎 추출물을 첨가하는 단계;를 포함하는 피부 상태 개선용 식품 조성물 제조방법에 관한 것이다. Another aspect of the present invention includes (a) mixing green tea extract with fish collagen at a weight ratio of 1:0.01 to 1:0.1; and (c) a complex amino acid containing glycine, L-glutamine, L-cysteine, and L-lysine mixed with the fish collagen in a ratio of 8 to 12: 3 to 6: 2 to 4: 0.5 to 2, and Dracocephalum moldavi. It relates to a method for producing a food composition for improving skin condition, comprising adding a kale leaf extract.
본 발명의 일 실시예에서 상기 (a) 및 (b) 단계의 피쉬 콜라겐은 녹차 추출물을 혼합하여 어린으로부터 유래할 수 있는 이취를 제거에 효과가 있음을 확인하였다(도 2). In one embodiment of the present invention, the fish collagen of steps (a) and (b) mixed with green tea extract was confirmed to be effective in removing off-flavors that may originate from young fish (FIG. 2).
본 발명의 피부 상태 개선용 식품 조성물을 첨가할 수 있는 식품은 건강기능성 보조식품, 비타민 복합제, 차, 음료수, 각종 스프, 낙농제품, 껌류, 라면, 기타 면류, 피자, 과자류, 캔디류, 스낵류, 초콜릿류, 빵, 소시지 등의 식품류, 정제, 캡슐, 환, 과립, 액상, 분말, 편상, 페이스트상, 시럽, 젤, 젤리, 바 등의 건강기능식품 제제류 등으로 제조될 수 있다. Foods to which the food composition for improving skin condition of the present invention can be added include health functional supplements, vitamin complexes, tea, beverages, various soups, dairy products, gums, ramen, other noodles, pizza, confectionery, candies, snacks, and chocolate. It can be manufactured into foods such as bread, sausages, and health functional food preparations such as tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, and bars.
본 발명의 피부 상태 개선용 식품 조성물은 법률상기능상의 구분에 있어서 제조유통 시점의 시행 법규에 부합하는 한 임의의 제품 구분을 띌 수 있다. 예컨대 한국 『건강기능식품에관한법률』에 따른 건강기능식품이거나, 한국 『식품위생법』의 식품공전(식약처 고시 『식품의 기준 및 규격』)상 각 식품유형에 따른 과자류, 두류, 다류, 음료류, 특수용도 식품 등일 수 있다. The food composition for improving skin condition of the present invention can be classified into any product classification in terms of legal function as long as it complies with the enforcement laws and regulations at the time of manufacture and distribution. For example, it is a health functional food according to the Korean 『Health Functional Foods Act』, or confectionery, beans, tea, and beverages according to each food type according to the food code of the Korean 『Food Sanitation Act』 (Ministry of Food and Drug Safety Notification 『Food Standards and Specifications』) , food for special purposes, etc.
상기 식품의 종류는 구체적으로 건강기능식품일 수 있다. 상기 건강기능식품은 여러 가지 영양제, 비타민 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 증진제, 펙트산 및 그의 염, 유기산, 보호성 콜로이드 점증제, pH 조절제, 안정화제, 보존제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 이러한 성분은 단독으로 또는 조합으로 사용될 수 있으며, 이러한 첨가제의 비율은 조성물 전체 중량당 0.001 내지 50 중량부의 범위에서 선택되는 것이 일반적이다.The type of food may specifically be a health functional food. The health functional foods include various nutrients, vitamins and minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and enhancers, pectic acid and its salts, organic acids, protective colloidal thickeners, pH regulators, and stabilizers. , preservatives, glycerin, alcohol, carbonating agents used in carbonated drinks, etc. These ingredients can be used alone or in combination, and the ratio of these additives is generally selected in the range of 0.001 to 50 parts by weight per total weight of the composition.
상기 건강기능식품은 식품의 생체 조절 기능을 강조한 식품으로 물리적, 생화학적, 생물공학적인 방법을 이용하여 특정 목적에 작용 및 발현하도록 부가가치를 부여한 식품이다. 이러한 건강기능 식품의 성분은 생체 방어와 신체 리듬의 조절, 질환의 방지 및 회복에 관계하는 신체 조절 기능을 생체에 대하여 충분히 발휘하도록 설계하여 가공하게 되며, 식품으로 허용 가능한 식품 보조 첨가제, 감미료 또는 기능성 원료를 함유할 수 있다.The above-mentioned health functional food is a food that emphasizes the bioregulatory function of food and is a food that has added value to act and express for a specific purpose using physical, biochemical, and biotechnological methods. The ingredients of these health functional foods are designed and processed to fully exert the body's regulatory functions related to biological defense, regulation of body rhythm, prevention and recovery of disease, and contain food auxiliary additives, sweeteners or functional ingredients that are acceptable as food. May contain raw materials.
상기 건강기능식품은 정제, 과립, 분말, 캡슐, 액상의 용액 및 환으로 이루어진 군에서 선택된 어느 하나의 제형일 수 있으나, 이에 제한되지 않는다. 구체적으로 상기 정제 형태의 건강기능식품은 피부 상태 개선용 식품 조성물, 부형제, 결합제, 붕해제 및 다른 첨가제 혼합물을 통상의 방법으로 과립화한 다음, 활택제 등을 넣어 압축 성형하거나, 상기 혼합물을 직접 압축 성형하여 제조할 수 있다. 또한, 상기 정제 형태의 건강기능식품은 필요에 따라 고미제 등을 함유할 수 있으며, 필요에 따라 적당한 제피제로 제피할 수도 있다.The health functional food may be any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills, but is not limited thereto. Specifically, the health functional food in the form of tablets is made by granulating a mixture of a food composition for improving skin condition, excipients, binders, disintegrants and other additives in a conventional manner, then adding a lubricant, etc. and compression molding, or the mixture is directly manufactured. It can be manufactured by compression molding. In addition, the health functional food in the form of tablets may contain bittering agents, etc., if necessary, and may be peeled with a suitable peeling agent, if necessary.
상기 캡슐 형태의 건강기능식품 중 경질캡슐제는 통상의 경질캡슐에 피부 상태 개선용 식품 조성물 및 부형제 등의 첨가제와의 혼합물 또는 그의 입상물 또는 제피한 입상물을 충진하여 제조할 수 있다. 연질캡슐제는 피부 상태 개선용 식품 조성물 및 부형제 등의 첨가제와의 혼합물을 젤라틴 등 캡슐기제에 충진하여 제조할 수 있다. 상기 연질캡슐제는 필요에 따라 글리세린 또는 솔비톨 등의 가소제, 착색제, 보존제 등을 함유할 수 있다.Among the capsule-type health functional foods, hard capsules can be manufactured by filling a regular hard capsule with a mixture of additives such as food compositions and excipients for improving skin condition, or their granules or peeled granules. Soft capsules can be manufactured by filling a mixture of additives such as food compositions for improving skin condition and excipients into a capsule base such as gelatin. The soft capsule may contain plasticizers such as glycerin or sorbitol, colorants, and preservatives, if necessary.
상기 환 형태의 건강기능식품은 피부 상태 개선용 식품 조성물, 부형제, 결합제, 붕해제 등의 혼합물을 적당한 방법으로 성형하여 조제할 수 있으며, 필요에 따라 백당이나 다른 적당한 제피제로 전분, 탈크 또는 적당한 물질로 환의를 입힐 수도 있다. The above-mentioned health functional food in the form of a pill can be prepared by molding a mixture of food compositions for improving skin condition, excipients, binders, disintegrants, etc. in an appropriate manner. If necessary, starch, talc, or other appropriate substances can be added with white sugar or other suitable fillers. You can also change your clothes with .
상기 과립 형태의 건강기능식품은 피부 상태 개선용 식품 조성물, 부형제, 결합제, 붕해제 등의 혼합물을 적당한 방법으로 입상으로 제조할 수 있으며, 필요에 따라 착향제, 고미제 등을 함유할 수 있다.The health functional food in the form of granules can be manufactured into granules from a mixture of food compositions for improving skin condition, excipients, binders, disintegrants, etc., by an appropriate method, and may contain flavoring agents, bittering agents, etc., if necessary.
상기 부형제, 결합제, 붕해제, 활택제, 고미제, 착향제 등에 대한 용어 정의는 당업계에 공지된 것으로 그 기능 등의 동일 내지 유사한 것들을 포함할 수 있다. Definitions of terms such as excipients, binders, disintegrants, lubricants, bittering agents, flavoring agents, etc. are known in the art and may include the same or similar functions, etc.
또한, 상기 식품의 종류는 식품 첨가제일 수 있으며, 상기 식품 첨가제는 식품의 제조, 가공 또는 보존을 위해 식품에 첨가, 혼합, 침윤 기타의 방법에 의해 사용되는 물질을 의미한다. 상기 식품 첨가제는 천연물과 합성품이 있으며, 기능과 용도에 따라 분류할 수 있다. 현재 한국에 식품첨가물로 허가되어 있는 품목은 화학적 합성품 370여종, 천연첨가물 50여종이며, 주로 용도에 따라 보존료, 살균제, 산화방지제, 착색료, 발색제, 표백제, 조미료, 감미료, 착향료, 팽창제, 강화제, 개량제, 유화제, 증점제(호료) 및 안정제, 피막제, 껌 기초제, 소포제, 용제, 이형제, 방충제, 품질개량제와 기타 식품제조용 첨가제 등으로 분류되어 쓰이고 있다.In addition, the type of food may be a food additive, and the food additive refers to a substance used by adding, mixing, infiltrating, or other methods into food for the production, processing, or preservation of food. The food additives include natural products and synthetic products, and can be classified according to their function and use. Currently, the items approved as food additives in Korea include over 370 types of chemical compounds and 50 types of natural additives. Depending on their use, they are mainly preservatives, disinfectants, antioxidants, colorants, colorants, bleaches, seasonings, sweeteners, flavoring agents, leavening agents, strengtheners, and improvers. , emulsifiers, thickeners and stabilizers, coating agents, gum bases, antifoaming agents, solvents, mold release agents, insect repellent, quality improvers and other food manufacturing additives.
상기 식품첨가제의 형태는 분말, 과립, 정제, 캡슐 또는 액상 형태를 포함할 수 있으나, 상기 형태에 제한되는 것은 아니다.The form of the food additive may include powder, granule, tablet, capsule, or liquid form, but is not limited to the above form.
본 발명의 피부 상태 개선용 식품 조성물을 사용할 경우, 상기 피부 상태 개선용 식품 조성물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용하고, 통상적인 방법에 따라 적절하게 사용할 수 있다. 상기 피부 상태 개선용 식품 조성물의 혼합량은 그의 사용 목적(예방, 건강개선 또는 치료적 처치)에 따라 적합하게 결정될 수 있다.When using the food composition for improving skin condition of the present invention, the food composition for improving skin condition can be added as is or used together with other foods or food ingredients, and can be used appropriately according to conventional methods. The mixing amount of the food composition for improving skin condition can be appropriately determined depending on the purpose of use (prevention, health improvement, or therapeutic treatment).
본 발명의 피쉬 콜라겐을 함유하는 식품 조성물은 피부 탄력, 피부 재생에 효과가 있으며, 콜라겐, 아미노산 및 드라코세팔룸 몰다비카 잎 추출물의 조합에 따라 염증 개선 및 피부 항산화에도 탁월한 효과가 있다.The food composition containing fish collagen of the present invention is effective in skin elasticity and skin regeneration, and has excellent effects in improving inflammation and skin antioxidant according to the combination of collagen, amino acids, and Dracocephalum moldavica leaf extract.
아울러 저분자 피쉬 콜라겐 함유로 체내 흡수율이 우수하며, 피쉬 콜라겐의 저분자화 과정에서 염기 처리를 최소화함으로써 섭취 시 안전하여 어린아이부터 노인까지 다양한 연령층의 섭취가 가능하다. In addition, because it contains low-molecular-weight fish collagen, it has an excellent absorption rate in the body, and by minimizing base treatment in the process of low-molecular-weight fish collagen, it is safe when ingested and can be consumed by various age groups, from children to the elderly.
본 발명의 효과는 상기 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 청구범위에 기재된 발명의 구성으로부터 추론 가능한 모든 효과를 포함하는 것으로 이해되어야 한다.The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.
도 1은 피부 상태 개선용 식품 조성물의 제조 공정도를 나타낸 것이다.
도 2는 이취 성분 함량 측정 결과를 나타낸 것이다. Figure 1 shows a manufacturing process diagram of a food composition for improving skin condition.
Figure 2 shows the results of measuring the content of off-flavor components.
이하, 첨부된 도면을 참고하여 본 발명의 실시예에 관하여 상세히 서술하나, 하기 실시예에 의해 본 발명이 제한되지 아니함은 자명하다Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings, but it is obvious that the present invention is not limited to the following examples.
실시예 1.Example 1.
1-1. 피쉬 콜라겐 분말의 준비1-1. Preparation of fish collagen powder
시중에서 구입한 피쉬 콜라겐(젤텍, 500 내지 3,000 Da)을 준비하였다. 상기 피쉬 콜라겐과 녹차 추출물을 1 : 0.01의 중량비로 혼합한 후 상온에서 30분 식힌 피쉬 콜라겐 수용액을 농축, 건조 및 분말화하여 피쉬 콜라겐 분말을 제조하였다. Commercially purchased fish collagen (Geltec, 500 to 3,000 Da) was prepared. Fish collagen powder was prepared by mixing the fish collagen and green tea extract at a weight ratio of 1:0.01, then cooling the fish collagen aqueous solution for 30 minutes at room temperature and concentrating, drying, and powdering it.
1-2. 드라코세팔룸 몰다비카(1-2. Dracocephalum moldavica ( Moldavian dragonheadMoldavian dragonhead ) 잎 추출물의 제조) Preparation of leaf extract
시중에서 구입한 드라코세팔룸 몰다비카(Moldavian dragonhead) 잎(이하 '몰다비카 잎' 이라고 함)을 깨끗하게 세척하였다. 세척한 몰다비카 잎은 약 400~500 um 크기로 분쇄하고, 약 10배 부피인 30%(w/v) 에탄올을 용매로 하여 상온에서 7일 동안 침적 추출하였다. 상기 추출물을 각각 여과지 (150 mm, Adventee, Osaka, Japan)로 여과한 뒤 감압농축(EYELA, Tokyo, Japan) 및 동결건조(Ilshin BioBase, Dongducheon, Korea)하여, 추출물의 고형분을 수득하였다. Commercially purchased Dracocephalum Moldavica ( Moldavian dragonhead ) leaves (hereinafter referred to as ‘Moldavica leaves’) were thoroughly washed. The washed Moldavica leaves were pulverized to a size of approximately 400-500 um and subjected to immersion extraction at room temperature for 7 days using approximately 10 times the volume of 30% (w/v) ethanol as a solvent. The above extracts were filtered through filter paper (150 mm, Adventee, Osaka, Japan), then concentrated under reduced pressure (EYELA, Tokyo, Japan) and freeze-dried (Ilshin BioBase, Dongducheon, Korea) to obtain the solid content of the extract.
1-3. 피쉬 콜라겐을 포함하는 복합 분말의 제조1-3. Preparation of composite powder containing fish collagen
상기 실시예 1-1에서 준비한 피쉬 콜라겐 분말에 글리신, L-글루타민, L-시스테인 및 L-라이신을 8~12 : 3~6 : 2~4 : 0.5~2의 비율로 포함하는 복합 아미노산과 실시예 1-2에서 제조한 몰다비카 잎 추출물을 혼합하였다. 이때 상기 혼합물은 피쉬 콜라겐 85~90 중량부, 복합 아미노산 8~15 중량부, 몰다비카 잎 추출물 0.01~0.1 중량부 비율로 포함한다. 이의 함량은 표 1에 자세히 나타내었다.Implemented with complex amino acids containing glycine, L-glutamine, L-cysteine and L-lysine in the fish collagen powder prepared in Example 1-1 in a ratio of 8 to 12: 3 to 6: 2 to 4: 0.5 to 2. The Moldavica leaf extract prepared in Example 1-2 was mixed. At this time, the mixture contains 85 to 90 parts by weight of fish collagen, 8 to 15 parts by weight of complex amino acids, and 0.01 to 0.1 parts by weight of moldavica leaf extract. Its content is shown in detail in Table 1.
이후 분무 건조기(Spray Dray, Niro atomizer, Copenhagen, Denmark)를 이용해 분무 건조하여 피쉬 콜라겐 복합 분말을 제조하였다. 분무 건조는 7,000rpm, 송풍온도 150℃, 배풍온도 90℃, 사입량 40rpm이며, 제조된 분말을 80~100 메쉬(mesh)로 제조하였다. Afterwards, fish collagen complex powder was prepared by spray drying using a spray dryer (Spray Dray, Niro atomizer, Copenhagen, Denmark). Spray drying was performed at 7,000 rpm, blowing temperature of 150°C, exhaust air temperature of 90°C, and feed rate of 40 rpm, and the produced powder was manufactured with 80 to 100 mesh.
실시예 2.Example 2.
실시예 1에서 복합 아미노산, 몰다비카 잎 추출물 혼합 시 전체 분말의 중량 대비 모과분말 0.01~0.05 중량부, 레몬분말 0.01 내지 0.05 중량부를 첨가하였다. In Example 1, when mixing complex amino acids and Moldavica leaf extract, 0.01 to 0.05 parts by weight of quince powder and 0.01 to 0.05 parts by weight of lemon powder were added relative to the weight of the total powder.
비교예 1.Comparative Example 1.
피쉬 콜라겐 제조과정에서 녹차 추출물과 1 : 0.01의 중량비로 혼합하는 단계를 생략하고, 그 외 복합 분말의 제조공정은 동일하다. In the fish collagen manufacturing process, the step of mixing with green tea extract at a weight ratio of 1:0.01 is omitted, and other than that, the manufacturing process for the composite powder is the same.
비교예 2.Comparative Example 2.
실시예 1과 제조방법은 동일하다. 다만, 몰다비카 잎 추출물을 제조 및 첨가하는 단계는 생략하였다. The manufacturing method is the same as Example 1. However, the steps of preparing and adding Moldavica leaf extract were omitted.
비교예 3.Comparative Example 3.
실시예 1과 제조방법은 동일하다. 다만, 몰다비카 잎 추출물 대신 유사한 형태의 꿀풀과 용머리속(Dracocephalum)의 용머리 꽃 추출물을 첨가하였다. The manufacturing method is the same as Example 1. However, instead of Moldavica leaf extract, a similar type of Lamiaceae genus Dracocephalum flower extract was added.
시중에서 구입한 용머리 꽃을 깨끗하게 세척하였다. 세척한 용머리 꽃을 약 400~500 um 크기로 분쇄하고, 약 10배 부피인 80%(w/v) 에탄올을 가한 후, 10분간 교반하여 균질화하였다. 상기 균질화된 용머리 꽃을 60 내지 80℃가 되도록 유지시키면서 초음파 추출기(Power sonica, 화신테크)를 이용하여 3시간동안 지속적으로 교반하면서 추출하였다. 상기 추출물을 각각 여과지 (150 mm, Adventee, Osaka, Japan)로 여과한 뒤 감압농축(EYELA, Tokyo, Japan) 및 동결건조(Ilshin BioBase, Dongducheon, Korea)하였다.Dragon head flowers purchased from the market were thoroughly washed. The washed dragon head flowers were pulverized to a size of approximately 400-500 um, approximately 10 times the volume of 80% (w/v) ethanol was added, and then stirred for 10 minutes to homogenize. The homogenized dragon head flowers were extracted using an ultrasonic extractor (Power Sonica, Hwashin Tech) while maintaining the temperature at 60 to 80°C while continuously stirring for 3 hours. The extracts were filtered through filter paper (150 mm, Adventee, Osaka, Japan), concentrated under reduced pressure (EYELA, Tokyo, Japan), and freeze-dried (Ilshin BioBase, Dongducheon, Korea).
제조예 1. 저분자 피쉬 콜라겐을 포함하는 젤리Preparation Example 1. Jelly containing low molecular weight fish collagen
실시예 1, 2 및 비교예 1 내지 3의 피부 개선용 식품 조성물 26 중량부, 플락토올리고당 10 중량부, 구연산 0.9 중량부, 구연산나트륨 0.3 중량부, 젖산칼슘 0.5 중량부, 푸드겔(겔화제) 1.7 중량부, 카라기난 0.1 중량부, 스테비올라배당체 0.15 중량부 및 정제수 59.65중량부의 중량비율로 혼합하여 탱크에 투입하였고, 85℃의 온도에서 30분 동안 교반하였다. 26 parts by weight of the food composition for skin improvement of Examples 1 and 2 and Comparative Examples 1 to 3, 10 parts by weight of plactooligosaccharide, 0.9 part by weight of citric acid, 0.3 part by weight of sodium citrate, 0.5 part by weight of calcium lactate, food gel (gelling agent) ) 1.7 parts by weight, 0.1 parts by weight of carrageenan, 0.15 parts by weight of steviola glycoside, and 59.65 parts by weight of purified water were mixed and placed in a tank, and stirred at a temperature of 85°C for 30 minutes.
이어서, 상기 교반된 재료들을 충진기에 충진한 후 27℃의 온도에서 냉각하여 피쉬 콜라겐을 함유한 젤리를 제조하였다. Next, the stirred ingredients were filled into a filler and cooled at a temperature of 27°C to prepare jelly containing fish collagen.
실험예 1. 이취 제거 효능Experimental Example 1. Off-flavor removal efficacy
상기 실시예 1 및 비교예 1, 2에서 제조된 식품 조성물의 이취 성분의 함량을 분석하였다. 이취 성분은 특정한 성분으로 정의되지 않기에 피쉬 콜라겐 제품 내 전체 향기 성분의 함량을 측정하였다. 향기 성분 함량은 headspace extraction 방법을 이용하여 추출하였고, 추출된 향기 성분은 SPME fiber에 흡착시킨 후 기체크로마토그래프/질량 분석기(GC/MS; Agilent Technologies/7890A GC/5975C MSD)를 통해 정량 분석하였다. 실시예 및 비교예의 조성물을 20 mL 바이알(vial)에 3 g을 담고, 60℃에서 20분간 가열한 후, headspace에 추출하였다. 추출된 향기 성분은 SPME fiber (Supelco; 65um PDMS/DVB blue plain (CAT No. 57311))를 사용하여 60℃, 30분간 fiber에 흡착시키고, GC주입구에 splitless mode로 주입하여 탈착한 후 GC/MS를 활용하여 향기 성분을 정량하였다. 향기성분의 정량분석은 TIC (Total Ion Current) 모드로 분석하였다. 분석 결과는 도 2에 나타내었다.The content of off-flavor components in the food compositions prepared in Example 1 and Comparative Examples 1 and 2 was analyzed. Since off-flavor components are not defined as specific components, the content of all aroma components in fish collagen products was measured. The aroma component content was extracted using the headspace extraction method, and the extracted aroma components were adsorbed onto SPME fiber and quantitatively analyzed using a gas chromatograph/mass spectrometer (GC/MS; Agilent Technologies/7890A GC/5975C MSD). 3 g of the compositions of Examples and Comparative Examples were placed in a 20 mL vial, heated at 60°C for 20 minutes, and then extracted into the headspace. The extracted fragrance component was adsorbed onto the fiber using SPME fiber (Supelco; 65um PDMS/DVB blue plain (CAT No. 57311)) at 60°C for 30 minutes, injected into the GC inlet in splitless mode, desorbed, and then subjected to GC/MS. Fragrance components were quantified using . Quantitative analysis of fragrance components was analyzed in TIC (Total Ion Current) mode. The analysis results are shown in Figure 2.
그 결과 피쉬콜라겐에 녹차 추출물과 혼합하여 이취성분 제어 효율이 향상되고, 몰다비카 잎 추출물을 처리함으로써 어린으로부터 유래할 수 있는 이취 성분을 상당 부분 제어할 수 있음을 확인하였다. As a result, it was confirmed that the efficiency of controlling off-flavor components was improved by mixing fish collagen with green tea extract, and that a significant portion of off-flavor components that may originate from children could be controlled by treating moldavica leaf extract.
실험예 2. 관능평가Experimental Example 2. Sensory evaluation
상기 실시예 1, 2 및 비교예 1, 2의 조성물을 첨가한 젤리(제조예 1)의 맛, 향, 색상 및 전반적인 기호도에 대한 관능적인 품질평가를 실시하였다. 본 식품의 관능적 식별 능력이 있는 20세 이상의 성인 10명을 선정하였으며, 관능적 품질의 평가 척도는 9점 채점법(9점: 아주좋다, 7점: 좋다, 5점: 보통이다, 3점: 조금 나쁘다, 1점: 아주 나쁘다)으로 하였다. Sensory quality evaluation was conducted on the taste, aroma, color, and overall preference of the jelly (Preparation Example 1) to which the compositions of Examples 1 and 2 and Comparative Examples 1 and 2 were added. Ten adults over the age of 20 who had the ability to sensory identify this food were selected, and the sensory quality evaluation scale was a 9-point scoring system (9 points: very good, 7 points: good, 5 points: average, 3 points: slightly bad) , 1 point: very bad).
그 결과, 몰다비카 잎 추출물을 첨가하지 않은 비교예 2의 경우 색상에서 관능평가의 차이는 미미하게 나타났으나, 맛과 전반적인 기호도에서 현저히 점수가 낮게 나타났다. 상기 표 2의 결과를 통해 몰다비카 잎 추출물을 첨가함에 따라 피쉬 콜라겐 특유의 이취 및 비린맛을 마스킹(masking)하는 효과를 가지며, 맛과 전반적인 기호도에서 소비자들의 선호도를 높일 수 있음을 확인하였다. 나아가 모과 및 레몬 분말을 첨가함으로써 맛, 향 등이 모두 개선되고, 전반적 기호도에서도 우수한 평가를 받음을 알 수 있었다. As a result, in the case of Comparative Example 2, in which moldavica leaf extract was not added, there was a slight difference in sensory evaluation in color, but the score in taste and overall preference was significantly lower. Through the results in Table 2, it was confirmed that adding Moldavica leaf extract had the effect of masking the unique off-flavor and fishy taste of fish collagen, and could increase consumer preference in terms of taste and overall preference. Furthermore, it was found that by adding quince and lemon powder, both taste and aroma were improved, and overall preference was also evaluated as excellent.
실험예 3. 세포 수준에서 피부 개선 효능 확인Experimental Example 3. Confirmation of skin improvement efficacy at the cellular level
3-1. 항산화 효능3-1. Antioxidant effect
실시예 및 비교예의 조성물을 정제수에 현탁하여 자유라디칼 소거 활성(free radical scavenging activity)을 평가하였다. DPPH 측정법은 억제제(inhibitor)가 안정한 라디칼 DPPH(2,2-Diphenyl-1-picrylhydrazyl radical)를 소거하여 탈색되는 정도를 540nm에서 흡광도를 측정하는 방법이다. 대조군은 비타민 C를 이용하였고, 라디칼 DPPH의 50% 소거하는 농도(SC50)를 측정하고, 이를 양성대조군과 비교하였다. 이때 SC50값이 낮을수록 DPPH 자유라디칼의 소거 활성이 높은 것을 의미한다. The compositions of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging activity. The DPPH measurement method is a method of measuring the absorbance at 540 nm to determine the extent to which an inhibitor is decolorized by scavenging the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical). Vitamin C was used as the control group, and the concentration (SC 50 ) that scavenges 50% of radical DPPH was measured and compared with the positive control group. At this time, the lower the SC 50 value, the higher the scavenging activity of DPPH free radicals.
그 결과, 실시예 1의 식품 조성물은 비교예 1 내지 3과 비교하여 항산화 효과가 우수함을 확인하였다. 특히 몰다비카 잎 추출물을 함유하지 않은 비교예 2와 차이가 크게 나타났으며, 몰다비카 잎 추출물 대신 용머리 꽃 추출물을 사용한 비교예 3과 비교하더라도 실시예 1에서 4% 이상 낮은 SC50값을 보이면서, 항산화 활성이 우수함을 확인하였다. As a result, it was confirmed that the food composition of Example 1 had excellent antioxidant effect compared to Comparative Examples 1 to 3. In particular, there was a significant difference from Comparative Example 2, which did not contain Moldavica leaf extract, and even compared to Comparative Example 3, which used Yongmeori flower extract instead of Moldavica leaf extract, the SC 50 value was lower by more than 4% in Example 1. It was confirmed that the antioxidant activity was excellent.
3-2. 피부 탄력 증가 효능 평가3-2. Evaluation of efficacy in increasing skin elasticity
실시예 1, 2 및 비교예 1 내지 3의 조성물을 정제수에 현탁하여 1.0%의 농도로 조성한 후, 콜라겐을 분해하는 MMP-1의 억제 효과를 측정하였다. Human Normal Fibroblast 세포를 24 well plate에 1X105 cell//well 접종하여 24시간 배양한 후, 적외선을 조사하여 MMP-1 발현을 증가시키고, 실시예 및 비교예 조성물을 농도별로 처리하여 MMP-1 억제 양상을 확인하였다. MMP-1 억제 효과는 하기 수학식 1을 통하여 평가하였으며, 콜라겐 생성 효능의 비교를 위한 양성대조군으로서 리테놀(비타민 A)을 사용하였다.The compositions of Examples 1 and 2 and Comparative Examples 1 to 3 were suspended in purified water to form a concentration of 1.0%, and then the inhibitory effect of MMP-1, which decomposes collagen, was measured. Human Normal Fibroblast cells were inoculated in a 24 well plate at 1 The pattern was confirmed. The MMP-1 inhibitory effect was evaluated using Equation 1 below, and retenol (vitamin A) was used as a positive control for comparison of collagen production efficacy.
[수학식 1][Equation 1]
MMP-1 저해활성(%) = 추출물 처리시 흡광도/추출물 미처리시 흡광도X100MMP-1 inhibitory activity (%) = absorbance when treated with extract/absorbance when not treated with extract
그 결과, 실시예 1 및 2의 시료는 비교예 1 내지 3 대비 MMP-1 저해 활성이 우수하게 나타남을 확인하였다. 피쉬 콜라겐을 녹차 추출물과 혼합함에 따라 MMP-1 억제 효과가 향상되고, 특히 몰다비카 잎 추출물의 첨가에 따라 MMP-1 억제 효과가 약 13% 이상 증가됨을 확인하여 실시예 1, 2의 조성물이 피부탄력 및 주름개선에 효과적임을 입증하였다.As a result, it was confirmed that the samples of Examples 1 and 2 exhibited superior MMP-1 inhibitory activity compared to Comparative Examples 1 to 3. As fish collagen was mixed with green tea extract, the MMP-1 inhibitory effect was improved. In particular, it was confirmed that the MMP-1 inhibitory effect was increased by more than about 13% by the addition of moldavica leaf extract, and the compositions of Examples 1 and 2 It has been proven to be effective in improving skin elasticity and wrinkles.
3-3. 콜라겐 생성 효능 평가3-3. Collagen production efficacy evaluation
인간 유래 섬유아세포(Fibroblast)를 배양하기 위해 DMEM과 10% FBS를 혼합한 것을 기본 배지로 하였다. 피부 섬유아세포를 48-well plate에 2~5x104 cells/ml의 농도로 분주하여 24시간 배양하였다. 성장 요인이 있는 기본 배지를 제거하고 실시예 1, 2 및 비교예 1 내지 3을 배치하고 24시간 배양하였다. 콜라겐 합성을 유도하는 TGF-B로 5 ng/ml이 되도록 처리하였다. 세포배양액을 취하여 Human procollagen1a1 duoset ELISA Kit(R&D Systems사)와 분광 광도계를 이용하여 합성된 콜라겐을 측정하였다. 분리 정제된 콜라겐을 0.25, 0.5, 1, 2, 4, 8 ng/ml의 농도에서 측정하여 검량선을 작성하였고 이를 바탕으로 실시예 1, 2, 비교예 1 내지 3, 음성대조군, 양성대조군의 콜라겐(Type I collagen) 생성량을 계산하였다. 메탄올로 처리된 군(음성대조군)에 비해 생성된 콜라겐 양을 콜라겐 생성율(%)로 계산하였으며, 실험은 3회 수행한 후 평균값을 계산하였다.To culture human-derived fibroblasts, a mixture of DMEM and 10% FBS was used as a basic medium. Skin fibroblasts were distributed in a 48-well plate at a concentration of 2~ 5x104 cells/ml and cultured for 24 hours. The basic medium containing growth factors was removed, and Examples 1 and 2 and Comparative Examples 1 to 3 were placed and cultured for 24 hours. It was treated with TGF-B, which induces collagen synthesis, at a concentration of 5 ng/ml. The cell culture medium was taken and synthesized collagen was measured using the Human procollagen1a1 duoset ELISA Kit (R&D Systems) and a spectrophotometer. The separated and purified collagen was measured at concentrations of 0.25, 0.5, 1, 2, 4, and 8 ng/ml to create a calibration curve, and based on this, the collagen of Examples 1 and 2, Comparative Examples 1 to 3, negative control, and positive control were prepared. (Type I collagen) production amount was calculated. The amount of collagen produced compared to the group treated with methanol (negative control group) was calculated as collagen production rate (%), and the experiment was performed three times and the average value was calculated.
[수학식 2][Equation 2]
콜라겐 생성율(%)=(각 실시예/비교예로 처리된 군의 콜라겐 생성량 = 메탄올 처리된 군의 콜라겐 생성량)/(메탄올로 처리된 군의 콜라겐 생성량) * 100Collagen production rate (%) = (Collagen production amount of the group treated with each example/comparative example = Collagen production amount of the methanol-treated group) / (Collagen production amount of the methanol-treated group) * 100
그 결과, 실시예 1 및 2에서 충분한 콜라겐 증진효과가 있음을 확인하였다. As a result, it was confirmed that there was a sufficient collagen enhancement effect in Examples 1 and 2.
3-4. 항염증 효능 평가3-4. Anti-inflammatory efficacy evaluation
콜라겐 매트릭스(Bioland, Korea)와 5배로 농축된 DMEM(Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), 2.2% 탄산수소나트륨, 200mM HEPES, 0.05N 수산화나트륨으로 제조된 완충용액을 7:2:1로 섞어 만든 인간 섬유아세포 cell line(ATCC, Cat, No. CRL-2076)을 밀도 1X105 세포/ml로 혼합한 뒤 직경 10 mm의 인써트에 각각 200 ul의 섬유아세포가 혼합된 콜라겐 용액을 넣어 진피를 제조하였다. 이후, 10% FPS를 함유하는 DMEM를 2일마다 교체하여 7일 동안 배양하였다. 배양된 진피 위에 피부자극물질인 SLS(sodium lauryl sulfate)를 10 ul 투여하고 4시간 뒤 실시예 1 및 비교예 1 내지 3을 각각 10 ul로 추가한 후 37℃, 5% CO2에서 24시간 동안 배양하였다. 이후 Human IL-1 assay kit(Endogen Inc. Boston, MA, USA) 및 ELISA-reader(enzyme-linked immunosorbent assay)를 이용하여 IL-1a의 방출량을 측정하였다. A buffer solution prepared with collagen matrix (Bioland, Korea), 5-fold concentrated DMEM (Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), 2.2% sodium bicarbonate, 200mM HEPES, and 0.05N sodium hydroxide was mixed at a ratio of 7:2:1. After mixing the human fibroblast cell line (ATCC, Cat, No. CRL-2076) at a density of 1 Manufactured. Afterwards, DMEM containing 10% FPS was replaced every 2 days and cultured for 7 days. 10 ul of SLS (sodium lauryl sulfate), a skin irritant, was administered onto the cultured dermis, and 4 hours later, 10 ul each of Example 1 and Comparative Examples 1 to 3 were added and incubated at 37°C and 5% CO 2 for 24 hours. Cultured. Afterwards, the amount of IL-1a released was measured using the Human IL-1 assay kit (Endogen Inc. Boston, MA, USA) and ELISA-reader (enzyme-linked immunosorbent assay).
그 결과, 표 6에 나타난 바와 같이 음성대조군에 비하여 SLS를 처리한 양성대조군에서 IL-1a의 방출이 현저하게 증가하였고, 실시예 1 및 비교예 1 내지 3과 비교하여 IL-1a의 방출이 감소하는 것을 확인할 수 있었다. 이는 본 발명의 식품 조성물이 고함량의 저분자 피쉬 콜라겐을 첨가함으로써 어느 정도 항염증 효과를 가지는 것으로 판단된다. 나아가 저분자 피쉬 콜라겐에 녹차 추출물의 처리 및 몰다비카 잎 추출물, 복합 아미노산과의 조합에 따라 IL-1a의 방출 효과를 현저히 개선시킨 것으로 판단된다. 전술한 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 예를 들어, 단일형으로 설명되어 있는 각 구성 요소는 분산되어 실시될 수도 있으며, 마찬가지로 분산된 것으로 설명되어 있는 구성 요소들도 결합된 형태로 실시될 수 있다.본 발명의 범위는 후술하는 청구범위에 의하여 나타내어지며, 청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.As a result, as shown in Table 6, the release of IL-1a was significantly increased in the positive control group treated with SLS compared to the negative control group, and the release of IL-1a was decreased compared to Example 1 and Comparative Examples 1 to 3. I was able to confirm that It is believed that the food composition of the present invention has a certain degree of anti-inflammatory effect by adding a high content of low-molecular-weight fish collagen. Furthermore, it is believed that treatment of low-molecular-weight fish collagen with green tea extract and combination with moldavica leaf extract and complex amino acids significantly improved the IL-1a release effect. The description of the present invention described above is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and similarly, components described as distributed may also be implemented in a combined form. The scope of the present invention is defined in the claims described below. It is indicated by, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.
Claims (1)
(a) 분자량 500 내지 3,000 Da의 분자량을 가지는 피쉬 콜라겐에 녹차 추출물을 1 : 0.01의 중량비로 혼합하는 단계;
(b) 상기 피쉬 콜라겐 85~90 중량부에 복합 아미노산 8~15 중량부 및 드라코세팔룸 몰다비카 잎 추출물 0.01~0.1중량부를 첨가하는 단계;
(c) 상기 (b) 단계가 종료된 뒤의 전체 중량대비 모과분말 0.01~0.05 중량부, 레몬분말 0.01 내지 0.05 중량부를 첨가하는 단계; 및
(d) 7,000rpm, 송풍온도 150℃, 배풍온도 90℃, 사입량 40rp 조건에서 분무건조하여 80~100메쉬(mesh)의 분말화 하는 단계;를 포함하고,
상기 복합 아미노산은 글리신, L-글루타민, L-시스테인 및 L-라이신을 8~12 : 3~6 : 2~4 : 0.5~2의 비율로 혼합한 것이다.A high-content collagen food composition with anti-oxidation, skin inflammation improvement, and skin elasticity-increasing effects, manufactured through the following steps:
(a) mixing green tea extract with fish collagen having a molecular weight of 500 to 3,000 Da at a weight ratio of 1:0.01;
(b) adding 8 to 15 parts by weight of complex amino acids and 0.01 to 0.1 parts by weight of Dracocephalum moldavica leaf extract to 85 to 90 parts by weight of the fish collagen;
(c) adding 0.01 to 0.05 parts by weight of quince powder and 0.01 to 0.05 parts by weight of lemon powder based on the total weight after step (b) is completed; and
(d) spray drying at 7,000 rpm, blowing temperature of 150°C, exhaust air temperature of 90°C, and feed rate of 40 rp to powderize the powder to 80 to 100 mesh;
The complex amino acid is a mixture of glycine, L-glutamine, L-cysteine and L-lysine in a ratio of 8 to 12: 3 to 6: 2 to 4: 0.5 to 2.
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| KR20180052980A (en) | 2016-11-11 | 2018-05-21 | 주식회사 뷰티콜라겐 | Composite powder comprising Seabuckthorn juice oil and collagen, method for preparing the same, and cosmetic compositions comprising the same |
| KR102301069B1 (en) | 2021-01-12 | 2021-09-13 | 정향훈 | Collagen jujube juice and manufacturing method of the same |
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| JP3766018B2 (en) * | 2001-12-17 | 2006-04-12 | 一丸ファルコス株式会社 | Active oxygen scavenger |
| KR101095698B1 (en) * | 2006-11-15 | 2011-12-20 | 가부시키가이샤 메이지 | Collagen peptide composition and food or beverage containing the same |
| KR102090971B1 (en) * | 2017-10-27 | 2020-03-19 | 주식회사 가인화장품 | Cosmetic composition |
| KR102358623B1 (en) * | 2020-11-16 | 2022-02-07 | 주식회사 미스케이 | Food composition for improving skin condition comprising collagen peptide |
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| KR20180052980A (en) | 2016-11-11 | 2018-05-21 | 주식회사 뷰티콜라겐 | Composite powder comprising Seabuckthorn juice oil and collagen, method for preparing the same, and cosmetic compositions comprising the same |
| KR102301069B1 (en) | 2021-01-12 | 2021-09-13 | 정향훈 | Collagen jujube juice and manufacturing method of the same |
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