KR20210065590A - Functional cosmetic composition comprising a mixed extract of Tanaka and pine needles - Google Patents

Functional cosmetic composition comprising a mixed extract of Tanaka and pine needles Download PDF

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KR20210065590A
KR20210065590A KR1020190154526A KR20190154526A KR20210065590A KR 20210065590 A KR20210065590 A KR 20210065590A KR 1020190154526 A KR1020190154526 A KR 1020190154526A KR 20190154526 A KR20190154526 A KR 20190154526A KR 20210065590 A KR20210065590 A KR 20210065590A
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tree
tanaka
pine
cosmetic composition
mixed extract
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KR102295962B1 (en
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도현훈
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주식회사 다솔
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/005—Preparations for sensitive skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures

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  • Engineering & Computer Science (AREA)
  • Cosmetics (AREA)

Abstract

The present invention relates to a functional cosmetic composition comprising a mixed extract of Citrus junos Tanaka and Pinus Densiflora leaves, and a method for producing the same, and specifically, to a functional cosmetic composition comprising a mixed extract of Citrus junos Tanaka and Pinus Densiflora leaves, which has antioxidant, antibacterial, skin soothing and anti-pollution effects.

Description

타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물{Functional cosmetic composition comprising a mixed extract of Tanaka and pine needles}Functional cosmetic composition comprising a mixed extract of Tanaka and pine needles

본 발명은 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물 및 그 제조방법에 관한 것으로서, 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물에 관한 것이다.The present invention relates to a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf, and a method for manufacturing the same, and is characterized in that it has antioxidant, antibacterial, skin soothing and anti-pollution effects. It relates to a functional cosmetic composition comprising a mixed extract.

현대사회로 접어들면서 사람들은 각종 유해환경에 지속적으로 노출되고 있으며, 각종 유해환경으로 인해 피부가 부적절한 영향을 받아 피부건강이 악화되는 문제점이 발생하고 있다.As we enter the modern society, people are continuously exposed to various harmful environments, and the skin is inappropriately affected by various harmful environments, resulting in deterioration of skin health.

상기와 같은 유해환경에 해당하는 것으로 활성산소, 각종 세균, 대기 중의 오염물질 등을 예로 들 수 있다.Examples of the harmful environment as described above include active oxygen, various bacteria, and pollutants in the air.

상기 활성산소는 생체 내에서 에너지 생산을 위한 산화 과정 중에 발생하는 것으로, 보통은 생체 내 제거 기작에 의해 대부분은 소멸되지만, 일부가 남아 피부 노화의 원인이 되곤 한다.The active oxygen is generated during the oxidation process for energy production in the living body, and most of them are usually eliminated by the in vivo removal mechanism, but some remain and cause skin aging.

또한, 상기 세균은 각종 염증의 원인이 되며, 피부에 트러블을 일으키는 바, 피부 건강에 악영향을 미치는 요소로 꼽히고 있다.In addition, the bacteria cause various inflammations and cause skin troubles, and are considered to be factors that adversely affect skin health.

또한, 대기 중의 오염 물질 또한, 피부에 지속적으로 접촉하여 피부의 건강을 악화시키는 원인이 되고 있다.In addition, pollutants in the air also continuously contact the skin, causing deterioration of the health of the skin.

상기와 같은 문제점을 해결하기 위해, 화학물질을 이용하여 활성산소, 각종 세균, 대기 중의 오염물질을 제거하려는 시도가 있었으나, 화학물질은 피부에 부작용을 나타낼 수 있고, 생산 단가가 비싸며, 생태학적으로 지속적이지 못하다는 문제점이 있었다.In order to solve the above problems, there have been attempts to remove active oxygen, various bacteria, and pollutants in the atmosphere using chemicals, but the chemicals may have side effects on the skin, are expensive, and ecologically There was a problem that it was not sustainable.

이에 따라, 다양한 천연물질을 이용하여 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 화장료 조성물을 얻어내기 위한 연구가 활발히 진행 중이다.Accordingly, research to obtain a cosmetic composition having antioxidant, antibacterial, skin soothing and anti-pollution effects using various natural substances is being actively conducted.

대한민국 등록특허공보 제10-1908975호(2018.10.17. 공고), "사위질빵 추출물을 유효성분으로 함유하는 안티폴루션 화장료 조성물"Republic of Korea Patent Publication No. 10-1908975 (Notice on October 17, 2018), "Anti-Pollution Cosmetic Composition Containing Sausage Bread Extract as an Active Ingredient" 대한민국 등록특허공보 제10-944025호(2019.01.30. 공고), "하늘타리 추출물을 유효성분으로 함유하는 안티폴루션 화장료 조성물"Republic of Korea Patent Publication No. 10-944025 (announced on January 30, 2019), "Anti-pollution cosmetic composition containing Haneultari extract as an active ingredient"

상기와 같은 문제점을 해결하기 위하여 본 발명은 타나까나무의 분말과 소나무잎을 혼합하여 추출한 혼합추출물을 유효성분으로 포함함으로써 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 제공하는데 목적이 있다.In order to solve the above problems, the present invention contains a mixed extract extracted by mixing the powder of the pine tree and the pine leaf as an active ingredient, thereby having antioxidant, antibacterial, skin soothing and anti-pollution effects. An object of the present invention is to provide a functional cosmetic composition comprising a mixed extract of

상기와 같은 과제를 달성하기 위하여 본 발명은 타나까나무의 분말과 소나무잎을 혼합하여 추출한 혼합추출물을 유효성분으로 포함하는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 제공한다.In order to achieve the above object, the present invention is a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf, characterized in that it contains a mixed extract extracted by mixing the powder of the Tanaka tree and the pine leaf as an active ingredient. provides

또한, 상기 타나까나무의 분말과 소나무잎은 30 : 70 ~ 70 : 30의 중량비로 혼합되는 것을 특징으로 한다.In addition, the powder of the Tanaka tree and the pine leaves are characterized in that they are mixed in a weight ratio of 30: 70 to 70: 30.

또한, 상기 타나까나무의 분말과 소나무잎은 100℃ 이하의 온도에서 열수 추출되는 것을 특징으로 한다.In addition, the powder and pine leaves of the Tanaka tree are characterized in that the hot water extraction at a temperature of 100 ℃ or less.

또한, 상기 혼합추출액은 전체 중량 대비 0.1 내지 10.0 중량%로 포함되는 것을 특징으로 한다.In addition, the mixed extract is characterized in that it is included in an amount of 0.1 to 10.0% by weight based on the total weight.

또한, 상기 화장료 조성물은 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 것을 특징으로 한다.In addition, the cosmetic composition is characterized in that it has antioxidant, antibacterial, skin soothing and anti-pollution effects.

본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물은 항산화, 항균, 피부진정 및 안티폴루션 효과를 가짐으로써 각종 유해환경으로부터 피부를 보호하는 효과가 있다.The functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf according to the present invention has antioxidant, antibacterial, skin soothing and anti-pollution effects, thereby protecting the skin from various harmful environments.

도 1은 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 혼합비율에 따른 항산화 효과를 나타낸 그래프.
도 2는 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 희석농도에 따른 항산화 효과를 나타낸 그래프.
도 3은 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 혼합비율에 따른 항균 효과를 나타낸 그래프.
도 4는 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 희석농도에 따른 항균 효과를 나타낸 그래프.
도 5은 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 혼합비율에 따른 피부진정 효과를 나타낸 그래프.
도 6는 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 희석농도에 따른 피부진정 효과를 나타낸 그래프.
도 7은 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 혼합비율에 따른 항오염 효과를 나타낸 그래프.
도 8는 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 희석농도에 따른 항오염 효과를 나타낸 그래프.1 is a graph showing the antioxidant effect according to the mixing ratio of the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.
Figure 2 is a graph showing the antioxidant effect according to the dilution concentration of the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.
Figure 3 is a graph showing the antibacterial effect according to the mixing ratio of the functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf according to the present invention.
Figure 4 is a graph showing the antibacterial effect according to the dilution concentration of the functional cosmetic composition comprising a mixed extract of the pine tree and pine leaves according to the present invention.
Figure 5 is a graph showing the skin soothing effect according to the mixing ratio of the functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf according to the present invention.
Figure 6 is a graph showing the skin soothing effect according to the dilution concentration of the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.
Figure 7 is a graph showing the anti-pollution effect according to the mixing ratio of the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.
Figure 8 is a graph showing the anti-pollution effect according to the dilution concentration of the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.

이하의 본 발명에 관한 상세한 설명들은 본 발명이 실시될 수 있는 실시 예이고 해당 실시 예의 예시로써 도시된 첨부 도면을 참조한다. 이들 실시 예는 당 업자가 본 발명의 실시에 충분하도록 상세히 설명된다. 본 발명의 다양한 실시 예는 서로 다르지만 상호 배타적일 필요는 없음이 이해되어야 한다. 예를 들어, 여기에 기재되어 있는 특정 형상, 구조 및 특성은 일 실시 예에 관련하여 본 발명의 사상 및 범위를 벗어나지 않으면서 다른 실시 예로 구현될 수 있다. 또한, 각각의 기재된 실시 예 내의 개별 구성요소의 위치 또는 배치는 본 발명의 사상 및 범위를 벗어나지 않으면서 변경될 수 있음이 이해되어야 한다.DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown as examples of the embodiments. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

따라서 후술되는 상세한 설명은 한정적인 의미로서 취하려는 것이 아니며, 본 발명의 범위는 적절하게 설명된다면 그 청구항들이 주장하는 것과 균등한 모든 범위와 더불어 첨부된 청구항에 의해서만 한정된다. 도면에서 유사한 참조부호는 여러 측면에 걸쳐서 동일하거나 유사한 기능을 지칭한다.Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

본 발명에서 사용되는 용어는 본 발명에서의 기능을 고려하면서 가능한 현재 널리 사용되는 일반적인 용어들을 선택하였으나, 이는 당 분야에 종사하는 기술자의 의도 또는 판례, 새로운 기술의 출현 등에 따라 달라질 수 있다. 또한, 특정한 경우는 출원인이 임의로 선정한 용어도 있으며, 이 경우 해당되는 발명의 설명 부분에서 상세히 그 의미를 기재할 것이다. 따라서 본 발명에서 사용되는 용어는 단순한 용어의 명칭이 아닌, 그 용어가 가지는 의미와 본 발명의 전반에 걸친 내용을 토대로 정의되어야 한다.The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in specific cases, there are also terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

본 발명에서 어떤 부분이 어떤 구성요소를 “포함”한다고 할 때, 이는 특별히 반대되는 기재가 없는 한, 다른 구성요소를 제외하는 것이 아니라 다른 구성요소를 더 포함할 수 있음을 의미한다.In the present invention, when a part "includes" a certain component, this means that other components may be further included, rather than excluding other components, unless otherwise stated.

이하, 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물에 대하여 자세히 설명한다.Hereinafter, it will be described in detail with respect to the functional cosmetic composition comprising a mixed extract of pine tree and pine leaves according to the present invention.

본 발명에 다른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물은 타나까나무의 분말과 소나무잎을 혼합하여 추출한 혼합추출물을 유효성분으로 포함하여 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 것을 특징으로 한다.According to the present invention, a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf contains a mixed extract extracted by mixing Tanaka tree powder and pine leaf as an active ingredient to provide antioxidant, antibacterial, skin soothing and anti-pollution properties. It is characterized by having an effect.

상기 타나까나무(Limonia Acidissima)는 미얀마, 태국, 히말라야 산맥, 인도 등의 지역에서 자라지만, 그 수량이 매우 적고 미얀마 슈에보, 버꼬꾸, 샨 지역 등이 주요 서식지로 알려져 있다. 상기 타나카나무는 강우량이 풍부한 곳에서 잘 자라며, 건조하고 바위가 많은 토질과 모래와 홍토가 섞여있는 곳에서 번식력이 우수하며, 나무의 재질은 돌과 같이 딱딱하지만 껍질은 얇으며 부드러운 특징을 가지고 있다. 따나카 나무의 지름이 3인치 정도 자라는 데에는 토양과 기후조건에 따라 3년에서 10년 정도의 기간이 소요되며, 화장품이나 의약품으로 이용되려면 최소 10년 이상 자란 나무야만이 그 가치로서 인정받을 수 있다. 본 발명에서는 타나까나무의 분말을 이용하며, 이때 타나까나무 분말은 타나까나무의 기둥으로 얻는 것이 바람직할 것이다.The Tanaka tree ( Limonia Acidissima ) grows in areas such as Myanmar, Thailand, Himalayas, and India, but its quantity is very small and Myanmar Suebo, Berkoku, Shan region and the like are known as major habitats. The Tanaka tree grows well in places with abundant rainfall, and has excellent fertility in dry, rocky soils and places where sand and red soil are mixed. The material of the tree is hard like stone, but the bark is thin and soft. . It takes 3 to 10 years for a Tanaka tree to grow about 3 inches in diameter, depending on the soil and climatic conditions, and only a tree that has grown for at least 10 years can be recognized for its value in order to be used as cosmetics or medicines. In the present invention, the powder of the Tanaka tree is used, and in this case, the powder of the Tanaka tree will be preferably obtained from the pillars of the Tanaka tree.

상기 소나무잎은 소나무(Pinus densiflora)의 잎, 즉, 솔잎을 지칭하는 것이다. 상기 소나무는 소나무과의 늘푸른 바늘잎 큰키나무로 키 20 ~ 35m 정도로 굵고 크게 자란다. 음지쪽 나무는 곧은 편이며 가지가 위와 옆으로 벋어 위쪽이 둥글거나 뾰족한 긴 삼각형이 된다. 상기 소나무는 낮은 산에서 높은 산까지 1,600m 이하 고지의 토질 좋은 곳에 서식하며, 주로 산허리 6부 능선 양지쪽에서 볼 수 있다. 상기 소나무잎은 길이 8~9cm 정도로 두 장씩 맞붙어 나오며, 가늘고 각진 바늘 모양이다. 난 지 2년 된 소나무잎은 아래쪽부터 갈색으로 변해 떨어지고 새잎이 나온다. 여름에는 짙은 녹색을 띠고, 겨울에는 노란빛 도는 녹색을 띤다.The pine leaves are pine ( Pinus densiflora ), that is, to refer to the pine needles. The pine is a tall tree with evergreen needles in the family Pineaceae, and grows thick and large about 20 to 35 m in height. The tree on the shade side is straight, and the branches extend upwards and downwards to form a long triangle with a rounded or pointed top. The pine tree inhabits in good soil quality in the highlands of 1,600 m or less from low to high mountains, and is mainly found on the sunny side of the 6th ridge of the mountainside. The pine leaves have a length of 8 to 9 cm and come out two by one, and have a thin and angular needle shape. Two-year-old pine leaves turn brown from the bottom and fall off, and new leaves emerge. It is dark green in summer and yellowish green in winter.

본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물은 상기 타나까나무의 분말과 소나무잎을 30 : 70 ~ 70 : 30의 중량비로 혼합하는 것을 특징으로 한다. 타나까나무의 분말과 소나무잎의 혼합 비율이 해당 범위를 벗어나면 항산화, 항균, 피부진정 및 안티폴루션 효과가 적절하게 나타나지 않는 문제점이 발생할 수 있다. 본 발명에 있어, 타나까나무의 분말과 소나무잎의 혼합 비율은 가장 적절하게는 50 : 50 중량비인 것이 가장 적절할 것이다.The functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf according to the present invention is characterized by mixing the powder of the Tanaka tree and the pine leaf in a weight ratio of 30: 70 to 70: 30. If the mixing ratio of Tanaka tree powder and pine leaf is out of this range, there may be a problem that the antioxidant, antibacterial, skin soothing and anti-pollution effects do not appear properly. In the present invention, the mixing ratio of the powder of Tanaka and pine leaves is most suitably 50: 50 weight ratio will be most appropriate.

본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물은 상기 타나까나무의 분말과 소나무잎을 100℃ 이하의 온도에서 열수 추출하는 것을 특징으로 한다. 상기 열수 추출의 온도가 100℃를 초과하면 타나까나무의 분말과 소나무잎 내부의 성분이 변하여 본 발명이 목적으로 하는 항산화, 항균, 피부진정 및 안티폴루션 효과가 제대로 나타나지 않는 문제점이 발생할 수 있다.Functional cosmetic composition comprising a mixed extract of pine tree and pine leaf according to the present invention is characterized in that the powder and pine leaf of the pine tree are extracted with hot water at a temperature of 100° C. or less. When the temperature of the hot water extraction exceeds 100° C., the components inside the powder and pine leaves of the Tanaka tree change, so that the antioxidant, antibacterial, skin soothing and anti-pollution effects targeted by the present invention may not appear properly. .

상기 타나까나무와 소나무잎의 혼합추출물은 전체 중량 대비 0.1 내지 10.0 중량%로 포함되는 것을 특징으로 한다. 상기 타나까나무와 소나무잎의 혼합추출물이 해당 범위를 벗어나 투입되면 항산화, 항균, 피부진정 및 안티폴루션 효과가 적절하게 나타나지 않는 문제점이 발생할 수 있다.The mixed extract of Tanaka tree and pine leaf is characterized in that it is included in an amount of 0.1 to 10.0% by weight based on the total weight. If the mixed extract of the pine tree and pine leaf is added outside the corresponding range, there may be a problem that the antioxidant, antibacterial, skin soothing and anti-pollution effects do not appear properly.

이하, 실시예 및 비교예 그리고 실험예를 통하여 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물이 갖는 효과에 대하여 자세히 설명한다.Hereinafter, the effects of the functional cosmetic composition comprising the mixed extract of the pine tree and pine leaves according to the present invention will be described in detail through Examples, Comparative Examples and Experimental Examples.

실시예Example 1. One. 타나까나무와with the tanaka tree 소나무잎의 혼합추출물을 포함하는 기능성 Functional containing extract of pine leaves 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐 실시예 1의 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 제조하였다.Through the following manufacturing process, a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf of Example 1 was prepared.

단계 S10: 타나까나무의 분말과 소나무잎을 50 : 50의 중량비로 혼합하여 혼합물을 제조하였다.Step S10: A mixture was prepared by mixing Tanaka tree powder and pine leaves in a weight ratio of 50:50.

단계 S20: 혼합물을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S20: The mixture was extracted with hot water at a temperature of 90° C. to prepare an extract.

단계 S30: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S30: The extract was separated by centrifugation to obtain a supernatant.

단계 S40: 상등액을 5.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S40: The supernatant was diluted in purified water to 5.0% by weight to complete the experimental preparation.

실시예Example 2 내지 2 to 3. 3. 타나까나무와with the tanaka tree 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 제조 Preparation of a functional cosmetic composition comprising a mixed extract of pine leaves

실시예 1의 제조과정에 따라 조성물을 제조하되, 단계 S10에서 타나까나무의 분말과 소나무잎을 각각 다른 혼합비율로 혼합하여 실시예 2 내지 3의 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 제조하였다.A composition was prepared according to the manufacturing process of Example 1, but in step S10, the powder and pine leaves were mixed at different mixing ratios, and the extracts of Tanaka trees and pine leaves of Examples 2 to 3 were mixed. A functional cosmetic composition was prepared.

타나까나무 분말의 함량Content of Tanaka tree powder 소나무잎의 함량content of pine leaves 실시예 2Example 2 30 중량%30% by weight 70 중량%70% by weight 실시예 3Example 3 70 중량%70% by weight 30 중량%30% by weight

실시예Example 4 내지 7. 4 to 7. 타나까나무와with the tanaka tree 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 제조 Preparation of a functional cosmetic composition comprising a mixed extract of pine leaves

실시예 1의 제조과정에 따라 조성물을 제조하되, 단계 S40에서 상등액이 정제수에 희석되는 비율을 각각 달리하여 실시예 4 내지 7의 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 제조하였다.A composition was prepared according to the manufacturing process of Example 1, but in step S40, the supernatant was diluted in purified water at different ratios to prepare a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf of Examples 4 to 7 did.

혼합추출물이 정제수에 희석되는 비율Ratio at which the mixed extract is diluted in purified water 실시예 4Example 4 0.1 중량%0.1% by weight 실시예 5Example 5 3.0 중량%3.0 wt% 실시예 6Example 6 7.0 중량%7.0 wt% 실시예 7Example 7 10.0 중량%10.0 wt%

비교예comparative example 1. 소나무잎 추출물을 포함하는 1. Containing pine leaf extract 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐, 타나까나무를 포함하지 않고 소나무잎 추출물만을 포함하는 비교예 1의 화장료 조성물을 제조하였다.Through the following manufacturing process, the cosmetic composition of Comparative Example 1 containing only the pine leaf extract was prepared without including the tanaca tree.

단계 S10: 타나까나무의 분말을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S10: Extraction was prepared by hot water extraction of the powder of Tanaka tree at a temperature of 90°C.

단계 S20: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S20: The extract was separated by centrifugation and the supernatant was taken.

단계 S30: 상등액을 5.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S30: The supernatant was diluted in purified water to 5.0% by weight to complete the experimental preparation.

비교예comparative example 2. 2. 타나까나무tanaka tree 추출물을 포함하는 containing extract 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐, 소나무잎을 포함하지 않고 타나까나무 추출물만을 포함하는 비교예 2의 화장료 조성물을 제조하였다.Through the following manufacturing process, the cosmetic composition of Comparative Example 2 was prepared which did not contain pine leaves and contained only the extract of Tanaka tree.

단계 S10: 타나까나무의 분말을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S10: Extraction was prepared by hot water extraction of the powder of Tanaka tree at a temperature of 90°C.

단계 S20: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S20: The extract was separated by centrifugation and the supernatant was taken.

단계 S30: 상등액을 5.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S30: The supernatant was diluted in purified water to 5.0% by weight to complete the experimental preparation.

비교예comparative example 3. 3. 타나까나무와with the tanaka tree 소나무잎이 pine leaves 10 : 90으로by 10:90 혼합된 혼합추출물을 포함하는 containing mixed extracts 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐 비교예 3의 화장료 조성물을 제조하였다.The cosmetic composition of Comparative Example 3 was prepared through the following manufacturing process.

단계 S10: 타나까나무의 분말과 소나무잎을 10 : 90의 중량비로 혼합하여 혼합물을 제조하였다.Step S10: A mixture was prepared by mixing Tanaka tree powder and pine leaves in a weight ratio of 10:90.

단계 S20: 혼합물을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S20: The mixture was extracted with hot water at a temperature of 90° C. to prepare an extract.

단계 S30: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S30: The extract was separated by centrifugation to obtain a supernatant.

단계 S40: 상등액을 5.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S40: The supernatant was diluted in purified water to 5.0% by weight to complete the experimental preparation.

비교예comparative example 4. 4. 타나까나무와with the tanaka tree 소나무잎이 pine leaves 90 : 10으로by 90:10 혼합된 혼합추출물을 포함하는 containing mixed extracts 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐 비교예 4의 화장료 조성물을 제조하였다.The cosmetic composition of Comparative Example 4 was prepared through the following manufacturing process.

단계 S10: 타나까나무의 분말과 소나무잎을 90 : 10의 중량비로 혼합하여 혼합물을 제조하였다.Step S10: A mixture was prepared by mixing Tanaka tree powder and pine leaves in a weight ratio of 90:10.

단계 S20: 혼합물을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S20: The mixture was extracted with hot water at a temperature of 90° C. to prepare an extract.

단계 S30: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S30: The extract was separated by centrifugation to obtain a supernatant.

단계 S40: 상등액을 5.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S40: The supernatant was diluted in purified water to 5.0% by weight to complete the experimental preparation.

비교예comparative example 5. 5. 타나까나무와with the tanaka tree 소나무잎의 혼합추출물이 0.01 The mixed extract of pine leaves is 0.01 중량%weight% 포함된 included 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐 비교예 5의 화장료 조성물을 제조하였다.The cosmetic composition of Comparative Example 5 was prepared through the following manufacturing process.

단계 S10: 타나까나무의 분말과 소나무잎을 50 : 50의 중량비로 혼합하여 혼합물을 제조하였다.Step S10: A mixture was prepared by mixing Tanaka tree powder and pine leaves in a weight ratio of 50:50.

단계 S20: 혼합물을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S20: The mixture was extracted with hot water at a temperature of 90° C. to prepare an extract.

단계 S30: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S30: The extract was separated by centrifugation to obtain a supernatant.

단계 S40: 상등액을 0.01 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S40: The supernatant was diluted in purified water to 0.01 wt% to complete the experimental preparation.

비교예comparative example 6. 6. 타나까나무와with the tanaka tree 소나무잎의 혼합추출물이 15.0 The mixed extract of pine leaves is 15.0 중량%weight% 포함된 included 화장료cosmetics 조성물의 제조 Preparation of the composition

하기의 제조과정을 거쳐 비교예 6의 화장료 조성물을 제조하였다.The cosmetic composition of Comparative Example 6 was prepared through the following manufacturing process.

단계 S10: 타나까나무의 분말과 소나무잎을 50 : 50의 중량비로 혼합하여 혼합물을 제조하였다.Step S10: A mixture was prepared by mixing Tanaka tree powder and pine leaves in a weight ratio of 50:50.

단계 S20: 혼합물을 90℃의 온도에서 열수 추출하여 추출물을 제조하였다.Step S20: The mixture was extracted with hot water at a temperature of 90° C. to prepare an extract.

단계 S30: 추출물을 원심분리기로 분리하여 상등액을 취했다.Step S30: The extract was separated by centrifugation to obtain a supernatant.

단계 S40: 상등액을 15.0 중량%가 되도록 정제수에 희석하여 실험준비를 완료하였다.Step S40: The supernatant was diluted in purified water to 15.0 wt% to complete the experimental preparation.

실험예Experimental example 1. 자유 라디칼 1. Free radicals 소거능extinction ability 실험 Experiment

항산화 효과 실험은 공지된 방법에 따라, DPPH법을 이용한 자유라디칼 소거 효과를 통해 실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물에 대한 항산화 효과를 확인하였다.The antioxidant effect test confirmed the antioxidant effect on the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 through the free radical scavenging effect using the DPPH method according to a known method.

실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물을 96 microplate에 100㎕ 씩 분주한 후, 에탄올을 이용하여 1mM이 되도록 용해한 2,2-diphenyl-1-picrylhydrazyl (DPPH) 용액을 10㎕ 첨가하였다. 상온에서 30분 반응 후 517nm에서 흡광도를 측정하여 free radical scavenging activity를 측정하였다. 0.1% 농도의 비타민C를 대조군으로 두어 실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물과 함께 실험하였다. 결과는 비타민C의 자유라디컬 소거효과를 100%로 기준하여 표 3 및 표 4에 나타내었다. 표 3은 타나까나무의 분말과 소나무잎의 혼합비율에 따른 실험결과를 나타낸 것이고, 표 4는 타나까나무의 분말과 소나무잎 혼합추출액이 포함된 농도에 따른 실험결과를 나타낸 것이다.After dispensing 100 μl of the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 into 96 microplates, 10 μl of a 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution dissolved in ethanol to 1 mM was added. did. After 30 minutes of reaction at room temperature, absorbance was measured at 517 nm to measure free radical scavenging activity. Vitamin C at a concentration of 0.1% was used as a control group and tested with the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6. The results are shown in Tables 3 and 4 based on the free radical scavenging effect of vitamin C as 100%. Table 3 shows the experimental results according to the mixing ratio of the powder of the pine tree and the pine leaf, and Table 4 shows the experimental results according to the concentration containing the powder of the pine tree and the pine leaf mixed extract.

타나까나무 분말의 혼합 중량비(중량 %)Mixing weight ratio of Tanaka tree powder (weight %) 소나무잎의 혼합 중량비(중량 %)Mixed weight ratio of pine leaves (weight %) 자유라디칼 소거효과(%)Free radical scavenging effect (%) 대조군(비타민C)Control group (vitamin C) -- -- 100%100% 비교예 1Comparative Example 1 00 100100 35.6%35.6% 비교예 3Comparative Example 3 1010 9090 60.4%60.4% 실시예 2Example 2 3030 7070 94.3%94.3% 실시예 1Example 1 5050 5050 99.8%99.8% 실시예 3Example 3 7070 3030 95.2%95.2% 비교예 4Comparative Example 4 9090 1010 73.5%73.5% 비교예 2Comparative Example 2 100100 00 43.1%43.1%

혼합추출물이 정제수에 희석된 비율(중량%)Ratio of mixed extract diluted in purified water (% by weight) 자유라디칼 소거효과(%)Free radical scavenging effect (%) 대조군(비타민C)Control group (vitamin C) -- 100%100% 비교예 5Comparative Example 5 0.010.01 56.4%56.4% 실시예 4Example 4 0.10.1 95.3%95.3% 실시예 5Example 5 3.03.0 97.5%97.5% 실시예 1Example 1 5.05.0 99.8%99.8% 실시예 6Example 6 7.07.0 98.7%98.7% 실시예 7Example 7 10.010.0 94.3%94.3% 비교예 6Comparative Example 6 15.015.0 78.9%78.9%

그 결과, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 우수한 자유라디컬 소거효과를 나타냄을 확인할 수 있었다.As a result, it can be confirmed that the powder of the pine tree and the pine leaf are mixed in a weight ratio of 30: 70 to 70: 30, and an excellent free radical scavenging effect is exhibited when the mixed extract is included in an amount of 0.1 to 10.0 wt% based on the total weight there was.

실험예Experimental example 2. 항균 효과 실험 2. Antibacterial effect test

실시예 1 내지 7 및 비교예 1 내지 6의 조성물에 대하여 항균효과를 측정하기 위해 항균 활성 실험에 사용한 세균은 병원성 세균으로, 그람 양성 세균으로 Staphylococcus aureus를 사용하였다. In order to measure the antibacterial effect of the compositions of Examples 1 to 7 and Comparative Examples 1 to 6, the bacteria used in the antibacterial activity experiment were pathogenic bacteria, and Staphylococcus aureus was used as the Gram-positive bacteria.

멸균 Mueller Hinton agar (Difco Co., USA) 배지에 각각의 균주 100μl씩 분주하여 균일하게 도포한 후 멸균 disk(φ 8mm)를 일정한 간격으로 배지에 밀착시켰다. 상기 실시예 1 내지 7 및 비교예 1 내지 6의 조성물을 disk에 떨어뜨린 후 37℃ 항온기에서 18시간 배양 후 억제대의 직경을 mm 단위로 측정하여 항균력을 비교하였다. 이때 대조군으로는 gentamicine(20μg)을 사용하였다. 각각의 조성물의 균주에 대한 생육억제 크기(inhibition zone)를 표 5 및 표 6에 나타냈다.100 μl of each strain was dispensed and uniformly applied to a sterile Mueller Hinton agar (Difco Co., USA) medium, and then a sterile disk (φ 8 mm) was adhered to the medium at regular intervals. After dropping the compositions of Examples 1 to 7 and Comparative Examples 1 to 6 on a disk, and incubating for 18 hours at 37°C in a thermostat, the diameter of the inhibition zone was measured in mm to compare the antibacterial activity. In this case, gentamicine (20 μg) was used as a control. The inhibition zone for each strain of each composition is shown in Tables 5 and 6.

타나까나무 분말의 혼합 중량비(중량 %)Mixing weight ratio of Tanaka tree powder (weight %) 소나무잎의 혼합 중량비(중량 %)Mixed weight ratio of pine leaves (weight %) Staphylococcus aureus에 대한 생육억제 크기(mm)Growth inhibition size for Staphylococcus aureus (mm) 비교예 1Comparative Example 1 00 100100 3.7mm3.7mm 비교예 3Comparative Example 3 1010 9090 8.3mm8.3mm 실시예 2Example 2 3030 7070 16.2mm16.2mm 실시예 1Example 1 5050 5050 18.3mm18.3mm 실시예 3Example 3 7070 3030 17.8mm17.8mm 비교예 4Comparative Example 4 9090 1010 9.2mm9.2mm 비교예 2Comparative Example 2 100100 00 4.4mm4.4mm

혼합추출물이 정제수에 희석된 비율(중량%)Ratio of mixed extract diluted in purified water (% by weight) Staphylococcus aureus에 대한 생육억제 크기(mm)Growth inhibition size for Staphylococcus aureus (mm) 비교예 5Comparative Example 5 0.010.01 3.2mm3.2mm 실시예 4Example 4 0.10.1 16.2mm16.2mm 실시예 5Example 5 3.03.0 17.3mm17.3mm 실시예 1Example 1 5.05.0 18.3mm18.3mm 실시예 6Example 6 7.07.0 17.7mm17.7mm 실시예 7Example 7 10.010.0 16.5mm16.5mm 비교예 6Comparative Example 6 15.015.0 5.3mm5.3mm

그 결과, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 우수한 항균 효과를 나타냄을 확인할 수 있었다.As a result, it was confirmed that the powder of the Tanaka tree and the pine leaf were mixed in a weight ratio of 30: 70 to 70: 30, and an excellent antibacterial effect was exhibited when the mixed extract was included in an amount of 0.1 to 10.0 wt % based on the total weight.

실험예Experimental example 3. 피부 진정 효과 실험 3. Skin soothing effect test

20세 ~ 25세의 건강한 성인남녀 50명을 대상으로 실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물을 이용하여 피부 진정효과를 평가하였다.The skin soothing effect was evaluated using the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 for 50 healthy adult men and women aged 20 to 25 years.

피시험자들의 전완부를 약 20분간 직사광선에 노출시킨 후, 피시험자들을 10명씩 5개조로 나누어, 4개조에 대해서는 상기 실시예 1 내지 7 및 비교예 1 내지 6에 의해 조성된 화장료에 부직포를 함침시킨 수딩패치를 피시험자들의 직사광선에 노출된 전완부에 20분간 부착하였으며, 나머지 1개조는 수딩패치로서 물에 함침시킨 수직포을 피시험자들의 직사광선에 노출된 전완부에 20분간 부착하여 대조군으로 하였다.After exposing the forearms of the test subjects to direct sunlight for about 20 minutes, the test subjects were divided into 5 groups of 10 people, and for the 4 groups, the nonwoven fabric was impregnated in the cosmetic composition according to Examples 1 to 7 and Comparative Examples 1 to 6 A soothing patch was attached to the forearms exposed to direct sunlight for 20 minutes, and the remaining one group was a soothing patch, and a vertical cloth impregnated with water was attached to the forearms exposed to direct sunlight of the subjects for 20 minutes as a control group.

피부의 진정효과 평가는, 상기 수딩패치를 부착하기 전, 수딩패치를 제거한 직후, 수딩패치를 제거하고 20분 후, 수딩패치를 제거하고 40분 후, 수딩패치를 제거하고 60분에 각각 온도를 측정하고 각 조의 평균값을 구하여 각 실시예에 의한 온도감소율을 측정하였다. 온도감소율은 하기 계산식 1을 이용하여 도출하였으며, 그 결과는 표 7 및 표 8에 나타내었다.To evaluate the soothing effect of the skin, before attaching the soothing patch, immediately after removing the soothing patch, 20 minutes after removing the soothing patch, 40 minutes after removing the soothing patch, remove the soothing patch and set the temperature at 60 minutes, respectively. The temperature reduction rate according to each example was measured by measuring the average value of each group. The temperature reduction rate was derived using the following formula 1, and the results are shown in Tables 7 and 8.

Figure pat00001
Figure pat00001

타나까나무 분말의 혼합 중량비(중량 %)Mixing weight ratio of Tanaka tree powder (weight %) 소나무잎의 혼합 중량비(중량 %)Mixed weight ratio of pine leaves (weight %) 수딩패치 제거 후 시간경과에 따른 온도변화율(%)Temperature change rate (%) over time after removing the soothing patch 0분0 minutes 20분20 minutes 40분40 minutes 60분60 minutes 대조군(물)Control (water) -- -- 7.27.2 0.60.6 0.40.4 0.20.2 비교예 1Comparative Example 1 00 100100 7.47.4 1.81.8 1.01.0 0.60.6 비교예 3Comparative Example 3 1010 9090 9.29.2 4.64.6 3.83.8 1.41.4 실시예 2Example 2 3030 7070 16.416.4 12.412.4 8.88.8 7.67.6 실시예 1Example 1 5050 5050 18.418.4 14.214.2 10.610.6 9.29.2 실시예 3Example 3 7070 3030 16.616.6 11.411.4 8.28.2 7.47.4 비교예 4Comparative Example 4 9090 1010 10.210.2 5.65.6 4.24.2 2.22.2 비교예 2Comparative Example 2 100100 00 7.67.6 2.22.2 1.41.4 0.80.8

혼합추출물이 정제수에
희석된 비율(중량%)Mixed extract in purified water
Diluted ratio (wt%) 수딩패치 제거 후 시간경과에 따른 온도변화율(%)Temperature change rate (%) over time after removing the soothing patch 0분0 minutes 20분20 minutes 40분40 minutes 60분60 minutes 대조군(물)Control (water) -- 7.27.2 0.60.6 0.40.4 0.20.2 비교예 5Comparative Example 5 0.010.01 7.47.4 1.21.2 1.01.0 0.80.8 실시예 4Example 4 0.10.1 16.216.2 11.211.2 8.88.8 8.08.0 실시예 5Example 5 3.03.0 17.417.4 13.213.2 9.29.2 8.48.4 실시예 1Example 1 5.05.0 18.418.4 14.214.2 10.610.6 9.29.2 실시예 6Example 6 7.07.0 17.817.8 13.813.8 9.89.8 8.68.6 실시예 7Example 7 10.010.0 16.616.6 11.411.4 9.09.0 8.28.2 비교예 6Comparative Example 6 15.015.0 8.48.4 4.24.2 2.42.4 1.81.8

그 결과, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 우수한 피부 진정 효과를 나타냄을 확인할 수 있었다.As a result, it was confirmed that the powder and pine leaves of Tanaka tree were mixed in a weight ratio of 30: 70 to 70: 30, and an excellent skin soothing effect was exhibited when the mixed extract was included in an amount of 0.1 to 10.0% by weight based on the total weight.

실험예Experimental example 4. 4. 안티폴루션anti-pollution 효과 실험 effect experiment

일반적으로 공해 물질과 직접적으로 맞닿게 되는 피부 각질세포를 사용하여 벤조피렌(2.5 ㎍/mL), 질산암모늄(500 ppm), 포름알데히드(1.5 ㎍/mL)에 의한 세포 손상 정도를 측정하였고, 이를 비교하여 피부 세포 손상 억제 효과를 확인하였다.In general, the degree of cell damage caused by benzopyrene (2.5 μg/mL), ammonium nitrate (500 ppm), and formaldehyde (1.5 μg/mL) was measured using skin keratinocytes that come in direct contact with pollutants, and compared them. Thus, the effect of inhibiting skin cell damage was confirmed.

사람 각질 세포 HaCaT를 1×104 cells/ml 농도로 96-well plate에 200 ㎕씩 분주하여 37℃, CO2 인큐베이터에서 24시간 배양하였다. 이때, 배지는 소혈청 10%와 항생제 1%가 포함된 IMDM 배지를 이용하였다. 배양 후 소혈청과 항생제가 배제된 IMDM 배지로 교체한 다음, 벤조피렌(2.5 ㎍/mL), 질산암모늄(500 ppm), 포름알데히드(1.5 ㎍/mL)를 처리한 후 실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물을 100 μg/ml 처리하여 21시간 추가 배양하였다. 그 후 5 mg/ml 농도의 MTT solution을 넣고 3시간 배양한 다음, 배지를 제거하고 dimethyl sulfoxide를 150 ul 씩 각 well에 넣어주고 10분간 shaking 하고, 570 nm로 흡광도를 측정하였다. 오염물을 처리하지 않은 실험군의 세포생존율을 대조군으로 하고, 대조군의 세포증식율을 100%로 하여 실시예 1 내지 7 및 비교예 1 내지 6의 화장료 조성물의 세포증식율을 비교하였다. 비교군은 실험군 대신 정제수만을 투입하였다.200 μl of human keratinocyte HaCaT at a concentration of 1×10 4 cells/ml was dispensed into a 96-well plate and cultured at 37° C. in a CO2 incubator for 24 hours. In this case, IMDM medium containing 10% bovine serum and 1% antibiotic was used as the medium. After culturing, the medium was replaced with an IMDM medium excluding bovine serum and antibiotics, and then treated with benzopyrene (2.5 μg/mL), ammonium nitrate (500 ppm), and formaldehyde (1.5 μg/mL) in Examples 1 to 7 and comparison The cosmetic compositions of Examples 1 to 6 were treated with 100 μg/ml and further cultured for 21 hours. After that, MTT solution of 5 mg/ml concentration was added and cultured for 3 hours. Then, the medium was removed and 150 ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570 nm. Cell viability of the experimental group not treated with contaminants was used as a control, and the cell proliferation rate of the control group was 100%, and the cell proliferation rates of the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 were compared. In the comparison group, only purified water was added instead of the experimental group.

타나까나무 분말의 혼합 중량비(중량 %)Mixing weight ratio of Tanaka tree powder (weight %) 소나무잎의 혼합 중량비(중량 %)Mixed weight ratio of pine leaves (weight %) 벤조피렌(2.5 ㎍/mL)에 대한 세포증식율(%)Cell proliferation rate (%) for benzopyrene (2.5 μg/mL) 질산암모늄(500 ppm)에 대한 세포증식율(%)Cell proliferation rate (%) for ammonium nitrate (500 ppm) 포름알데히드(1.5 ㎍/mL)에 대한 세포증식율(%)Cell proliferation rate (%) for formaldehyde (1.5 μg/mL) 대조군(오염물질 미처리군)Control group (untreated contaminant group) -- -- 100%100% 100%100% 100%100% 비교군(실험군 미처리군)Comparative group (experimental group, untreated group) 00 00 42%42% 44%44% 40%40% 비교예 1Comparative Example 1 00 100100 51%51% 52%52% 50%50% 비교예 3Comparative Example 3 1010 9090 63%63% 62%62% 63%63% 실시예 2Example 2 3030 7070 83%83% 81%81% 81%81% 실시예 1Example 1 5050 5050 89%89% 87%87% 84%84% 실시예 3Example 3 7070 3030 82%82% 84%84% 82%82% 비교예 4Comparative Example 4 9090 1010 64%64% 61%61% 65%65% 비교예 2Comparative Example 2 100100 00 54%54% 55%55% 52%52%

혼합추출물이 정제수에
희석된 비율(중량%)Mixed extract in purified water
Diluted ratio (wt%) 벤조피렌(2.5 ㎍/mL)에 대한 세포증식율(%)Cell proliferation rate (%) for benzopyrene (2.5 μg/mL) 질산암모늄(500 ppm)에 대한 세포증식율(%)Cell proliferation rate (%) for ammonium nitrate (500 ppm) 포름알데히드(1.5 ㎍/mL)에 대한 세포증식율(%)Cell proliferation rate (%) for formaldehyde (1.5 μg/mL) 대조군(오염물질 미처리군)Control group (untreated contaminant group) -- 100%100% 100%100% 100%100% 비교군(실험군 미처리군)Comparative group (experimental group, untreated group) -- 42%42% 44%44% 40%40% 비교예 5Comparative Example 5 0.010.01 48%48% 51%51% 51%51% 실시예 4Example 4 0.10.1 81%81% 81%81% 80%80% 실시예 5Example 5 3.03.0 83%83% 84%84% 82%82% 실시예 1Example 1 5.05.0 89%89% 87%87% 84%84% 실시예 6Example 6 7.07.0 84%84% 85%85% 83%83% 실시예 7Example 7 10.010.0 82%82% 83%83% 81%81% 비교예 6Comparative Example 6 15.015.0 49%49% 53%53% 50%50%

그 결과, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 우수한 항오염효과를 나타냄을 확인할 수 있었다.As a result, it was confirmed that the powder and pine leaves of Tanaka tree were mixed in a weight ratio of 30: 70 to 70: 30, and excellent anti-pollution effect was exhibited when the mixed extract was included in an amount of 0.1 to 10.0 wt % based on the total weight.

결론.conclusion.

상기 실험예 1 내지 4를 통해 본 발명에 따른 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물의 효과에 대하여 확인하였다.Through the above Experimental Examples 1 to 4, the effect of the functional cosmetic composition comprising the mixed extract of the pine tree and pine leaves according to the present invention was confirmed.

상기 실험예 1 내지 4를 통해 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 높은 자유라디컬 소거효과를 나타냄을 확인할 수 있었으며, 이를 통해 항산화 효과를 유추할 수 있었다.High free radical scavenging effect when the powder of Tanaka tree and pine leaf are mixed in a weight ratio of 30: 70 to 70: 30 through Experimental Examples 1 to 4, and the mixed extract is included in an amount of 0.1 to 10.0% by weight relative to the total weight was confirmed, and the antioxidant effect could be inferred from this.

또한, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 그람양성균에 대한 억제효과를 확인하였으며, 이를 통해 항균 효과를 유추할 수 있었다.In addition, when the powder of Tanaka tree and pine leaf are mixed in a weight ratio of 30: 70 to 70: 30, and the mixed extract is included in an amount of 0.1 to 10.0% by weight relative to the total weight, the inhibitory effect on Gram-positive bacteria was confirmed, through this antibacterial effect could be inferred.

또한, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 피부냉감 효과를 확인할 수 있었으며, 이를 통해 우수한 피부 진정 효과를 나타냄을 유추할 수 있었다.In addition, it was possible to confirm the skin cooling effect when the powder of Tanaka tree and the pine leaf were mixed in a weight ratio of 30: 70 to 70: 30, and the mixed extract was included in an amount of 0.1 to 10.0% by weight based on the total weight, and through this, excellent skin It could be inferred that it had a sedative effect.

또한, 타나까나무의 분말과 소나무잎이 30 : 70 ~ 70 : 30의 중량비로 혼합되고, 혼합추출액이 전체 중량 대비 0.1 내지 10.0 중량%로 포함될 때 벤조피렌, 질산암모늄 및 포름알데히드에 대해 우수한 세포증식율을 나타냄을 확인할 수 있었고, 이를 통해 우수한 항오염 효과를 가짐을 유추할 수 있었다. In addition, when the powder of Tanaka tree and the pine leaf are mixed in a weight ratio of 30: 70 to 70: 30, and the mixed extract is included in 0.1 to 10.0% by weight relative to the total weight, excellent cell proliferation rate for benzopyrene, ammonium nitrate and formaldehyde It could be confirmed that it shows, and it could be inferred that it had an excellent anti-pollution effect.

특히, 타나까나무추출물과 소나무잎추출물 각각의 효능의 합산치에 혼합 추출물의 효능이 높게 나타남을 확인하였고, 이를 통해 타나까나무추출물과 소나무잎추출물을 혼합하는 과정에서 예기치 못한 효과를 나타냈음을 확인할 수 있었다.In particular, it was confirmed that the efficacy of the mixed extract was high in the sum of the respective efficacy of the pine tree extract and the pine leaf extract, and through this, it was confirmed that an unexpected effect was exhibited in the process of mixing the pine tree extract and the pine leaf extract. could

따라서, 본 발명은 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 신규한 조성물로서, 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물을 개발하였음을 명시한다.Therefore, the present invention specifies that a functional cosmetic composition comprising a mixed extract of pine tree and pine leaves has been developed as a novel composition having antioxidant, antibacterial, skin soothing and anti-pollution effects.

본 발명을 첨부된 도면과 함께 설명하였으나, 이는 본 발명의 요지를 포함하는 다양한 실시 형태 중의 하나의 실시 예에 불과하며, 당 업계에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 하는 데에 그 목적이 있는 것으로, 본 발명은 상기 설명된 실시예에만 국한되는 것이 아님은 명확하다. 따라서, 본 발명의 보호범위는 하기의 청구범위에 의해 해석되어야 하며, 본 발명의 요지를 벗어나지 않는 범위 내에서 변경, 치환, 대체 등에 의해 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리에 포함될 것이다. 또한, 도면의 일부 구성은 구성을 보다 명확하게 설명하기 위한 것으로 실제보다 과장되거나 축소되어 제공되는 것임을 명확히 한다.Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent range by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality to more clearly describe the components.

Claims (5)

타나까나무와 소나무잎을 혼합하여 추출한 혼합추출물을 유효성분으로 포함하는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물.
A functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf, characterized in that it contains a mixed extract extracted by mixing Tanaka tree and pine leaf as an active ingredient.
 제1항에 있어서,
상기 타나까나무와 소나무잎은 30 : 70 ~ 70 : 30의 중량비로 혼합되는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물.
According to claim 1,
The Tanaka tree and the pine leaf are 30: 70 to 70: Functional cosmetic composition comprising a mixed extract of the Tanaka tree and pine leaf, characterized in that it is mixed in a weight ratio of 30.
 제1항에 있어서,
상기 타나까나무와 소나무잎은 100℃ 이하의 온도에서 열수 추출되는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물.
According to claim 1,
The functional cosmetic composition comprising a mixed extract of the thanaka tree and pine leaf, characterized in that the Tanaka tree and pine leaf are extracted with hot water at a temperature of 100° C. or less.
 제1항에 있어서,
상기 혼합추출액은 전체 중량 대비 0.1 내지 10.0 중량%로 포함되는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물.
According to claim 1,
The mixed extract is a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf, characterized in that it is contained in an amount of 0.1 to 10.0% by weight based on the total weight.
 제1항에 있어서,
상기 화장료 조성물은 항산화, 항균, 피부진정 및 안티폴루션 효과를 갖는 것을 특징으로 하는 타나까나무와 소나무잎의 혼합추출물을 포함하는 기능성 화장료 조성물.

According to claim 1,
The cosmetic composition is a functional cosmetic composition comprising a mixed extract of Tanaka tree and pine leaf, characterized in that it has antioxidant, antibacterial, skin soothing and anti-pollution effects.
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Publication number Priority date Publication date Assignee Title
KR20070119296A (en) * 2006-06-15 2007-12-20 황보식 Agent having effects of skin aging resister and skin whitening, manufactured using extract of pine tree as its main ingredient
KR20090087223A (en) * 2008-02-12 2009-08-17 주식회사 코리아나화장품 Cosmetic composition for pore-minimizing containing limonia acidissima extract
KR100944025B1 (en) 2008-01-16 2010-02-24 주식회사 우양이엔지 Air pulse type dust collector using ceramic filter
JP2011153120A (en) * 2010-01-27 2011-08-11 Otomo Sawako Method for making thanakha powder most effective
KR20120019551A (en) * 2010-08-26 2012-03-07 신라대학교 산학협력단 A method of preparing antioxidants having antimicrobial activity from pine trees
KR101908975B1 (en) 2018-07-04 2018-10-17 주식회사 아미코스메틱 A cosmetic compositon for anti-pollution containing extracts of clematis apiifolia

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070119296A (en) * 2006-06-15 2007-12-20 황보식 Agent having effects of skin aging resister and skin whitening, manufactured using extract of pine tree as its main ingredient
KR100944025B1 (en) 2008-01-16 2010-02-24 주식회사 우양이엔지 Air pulse type dust collector using ceramic filter
KR20090087223A (en) * 2008-02-12 2009-08-17 주식회사 코리아나화장품 Cosmetic composition for pore-minimizing containing limonia acidissima extract
JP2011153120A (en) * 2010-01-27 2011-08-11 Otomo Sawako Method for making thanakha powder most effective
KR20120019551A (en) * 2010-08-26 2012-03-07 신라대학교 산학협력단 A method of preparing antioxidants having antimicrobial activity from pine trees
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