KR20210042451A - Cosmetic composition comprising an extract of Undaria Pinnatifida Sporophyll Fermentation having anti-oxidative activity - Google Patents
Cosmetic composition comprising an extract of Undaria Pinnatifida Sporophyll Fermentation having anti-oxidative activity Download PDFInfo
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Abstract
Description
본 발명은 항산화 활성을 갖는 미역귀(Undaria Pinnatifida Sporophyll) 발효추출물을 함유하는 피부 노화 방지 및 개선을 위한 기능성 화장료 조성물 제조 방법에 관한 것으로, 보다 상세하게는 미역귀추출물을 발효시켜 기존의 미역귀발효물의 항염, 항산화 효능을 향상시킨 화장료 조성물 제조 방법에 관한 것이다.The present invention relates to a method for manufacturing a functional cosmetic composition for preventing and improving skin aging containing a fermented extract of seaweed ear (Undaria Pinnatifida Sporophyll) having antioxidant activity, and in more detail, the anti-inflammatory of the existing seaweed ear fermentation by fermenting the seaweed ear extract, It relates to a method for producing a cosmetic composition with improved antioxidant efficacy.
초기의 해양 성분 제품에 대한 연구는 주로 해조류나 게 껍질 등으로 몇몇의 소재들에 한정되었으나 최근에는 해양 심층수에서 뽑아낸 신물질을 이용하여 제품 개발에 활용함으로써 범위가 점차 다양해지고 있다. 다양한 형태의 해양관련 화장품이 유통중이지만, 해양 생물자원의 활용은 미비한 실정이며 실질적인 기능성 효과 또한 부족하다. 해조류의 경우 쉽게 구할 수 있으며 비용이 저렴하여 화장품 원료로서 부가가치가 높지만 활용되지 못하고 있다. 대부분의 해양 추출물 화장품은 초도 생산 수준에서 멈춰있으며, 원료의 추출과정과 수급에 어려움이 있어 생산의 지속이 어려운 실정이다. 본 발명은 원료의 지속적인 생산과 효율적인 추출법 개발을 통해 해조류 기능성 화장품 산업에 크게 기여하고자 한다.In the early years, research on marine component products was limited to a few materials, mainly seaweed and crab shells, but recently, new materials extracted from deep ocean water are used for product development, and the scope is gradually diversifying. Although various types of marine cosmetics are in circulation, the use of marine biological resources is insufficient and practical functional effects are also insufficient. In the case of seaweed, it is readily available and has a high added value as a cosmetic ingredient because it is inexpensive, but it is not being utilized. Most of the marine extract cosmetics have stopped at the initial production level, and it is difficult to continue production due to difficulties in the extraction process and supply of raw materials. The present invention intends to greatly contribute to the seaweed functional cosmetics industry through continuous production of raw materials and the development of an efficient extraction method.
최근 들어 해양 천연물 자원은 환경오염과 생산한계가 있는 육상생물자원의 대체자원으로서의 중요성이 부각되면서 해양 천연물로 부터 신소재를 개발하기 위한 연구가 여러 방면에서 진행되고 있다. 또한 고령화 사회 진입과 안티에이징에 대한 소비자들의 관심도가 높아지면서 화장품 산업에 바이오기술(기능성 소재등) 접목이 가속화 되고 있어 생물이 자연적으로 만들어내는 성분을 바이오 기술을 이용해 적용한 바이오 화장품의 기대가 점점 높아지고 있다. 해양자원 중 20% 이상을 차지하는 해조류에는 항암, 항산화, 항염 등 다양한 생리활성을 갖는 물질뿐만 아니라 미백, 보습, 주름개선 등의 피부 관련 기능성 활성물질이 함유되어 있다. 하지만 다양한 생리활성 효과는 알려져 있으나 화장품 원료로써의 연구는 극히 드물어 학술적인 가치가 매우 클 뿐만 아니라 원천 기술로서의 그 가치도 매우 중요하다. 해조류 추출물 중 감태(Ecklonia cava) 추출물은 항산화, 미백, 자외선 차단 등 다양한 효과에 대해 입증되었으며, 화장품으로 응용되고 있다. 미역귀(Undaria Pinnatifida Sporophyll) 추출물 발효물은 현재 항염증, 항산화에 관한 연구가 진행되고 있으며, 기능성 평가를 위해 총 폴리페놀과 플라보노이드 함량도 측정하였다. 하지만 아직 화장품 원료로서의 응용은 극히 드물며, 안정성 확인 및 제형화에 대한 연구가 필요할 것으로 예상된다.Recently, as the importance of marine natural product resources as an alternative resource for terrestrial biological resources with environmental pollution and production limitations has emerged, research to develop new materials from marine natural products is being conducted in various fields. In addition, as consumers' interest in anti-aging and entry into an aging society is increasing, biotechnology (functional materials, etc.) is accelerating in the cosmetic industry. have. Seaweed, which accounts for more than 20% of marine resources, contains not only substances having various physiological activities such as anti-cancer, antioxidant, and anti-inflammatory, but also skin-related functional active substances such as whitening, moisturizing, and wrinkle improvement. However, although various physiologically active effects are known, research as a cosmetic ingredient is extremely rare, so not only is the academic value very high, but its value as a source technology is also very important. Among seaweed extracts, Ecklonia cava extract has been proven for various effects such as antioxidant, whitening, and UV protection, and has been applied in cosmetics. For the fermented product of Undaria Pinnatifida Sporophyll extract, studies on anti-inflammatory and antioxidant are currently being conducted, and total polyphenol and flavonoid contents were also measured for functional evaluation. However, its application as a cosmetic raw material is still extremely rare, and it is expected that studies on stability confirmation and formulation are required.
상기와 같은 본 발명의 목적은 항산화 효능을 갖는 미역귀발효추출물을 수득한다.An object of the present invention as described above is to obtain a seaweed ear fermentation extract having an antioxidant effect.
본 발명은 미역귀발효추출물을 유효성분으로 함유하는 피부 노화 방지 화장품 조성물을 제공한다.The present invention provides an anti-aging cosmetic composition containing the seaweed ear fermentation extract as an active ingredient.
본 발명에 있어서, “유효성분”이라 함은 내재된 작용에 의해 그 기능성 화장품 조성물의 효능효과를 직접 또는 간접적으로 발현한다고 기대되는 물질 또는 물질군 (기능적 활성성분 등이 밝혀지지 않은 화장료를 포함한다)으로서 주성분을 포함하는 것을 의미한다.In the present invention, the term "active ingredient" includes a substance or group of substances that is expected to directly or indirectly express the efficacy effect of the functional cosmetic composition by its intrinsic action (a cosmetic composition for which a functional active ingredient, etc. is not known. It means to include the main component as ).
본 발명에서 미역귀발효추출물 분말은 1) 미역귀를 상온에서 세척수로 3회 세척한 후 물기를 자연 건조하는 단계와, 2) 5% 미역귀(미역귀 분쇄물100g, 증류수 2L를 121℃의 가압조건에서 15분동안 가열추출하는 단계와, 3)추출물을 여과하여 미역귀 분쇄물을 제거하고 여과액을 회수하는 단계와, 4)발효물을 추출하기 위해 1%의 탄소원으로 glicuse를 첨가한 MRS 액체배지를 121℃ 의 가압조건에서 15분간 멸균하는 단계와, 5)발효균(Lactobacillus breveis, Lactobacillus planntarum, Lactobacillus casie)을 1%농도로 접종하여 37℃ 배양기에서 24시간 배양하는 단계와, 6)앞 단계에서 추출했던 미역귀추출물에 균주배양액(Lactobacillus breveis, Lactobacillus planntarum, Lactobacillus casie)을 10%씩 각각 접종하여 37℃ 배양기에서 48시간 배양하여 미역귀 발효액을 만드는 단계와, 7)3종의 미역귀발효액과 미역귀열수추출물을 진공농축시킨 후 동결건조시켜 분말을 얻는 것을 특징으로 한다. In the present invention, the seaweed ear fermented extract powder is 1) washed three times with washing water at room temperature and then naturally dried, and 2) 5% seaweed ear (100 g of seaweed ear crushed product, 2L of distilled water 15 Heat extraction for minutes, 3) filtering the extract to remove the crushed seaweed and recovering the filtrate, and 4) MRS liquid medium containing glicuse as a 1% carbon source to extract the fermented product. Sterilizing for 15 minutes under pressurized conditions of ℃, 5) inoculating fermentation bacteria (Lactobacillus breveis, Lactobacillus planntarum, Lactobacillus casie) at a concentration of 1% and incubating for 24 hours in a 37°C incubator, and 6) extracted in the previous step. Inoculation of 10% of strain culture solution (Lactobacillus breveis, Lactobacillus planntarum, Lactobacillus casie) to the seaweed ear extract and incubating for 48 hours in a 37℃ incubator to make seaweed ear fermentation broth and 7) 3 kinds of seaweed ear fermentation broth and seaweed ear hot water extract vacuum It is characterized in that it is concentrated and then lyophilized to obtain a powder.
본 발명의 미역귀발효추출물은 플라보노이드를 함유하며 항산화 효능을 가진다. 또한 콜라겐 분해효소인 콜라게나아제 활성을 저해함으로써, 이를 유효성분으로 함유하는 조성물은 피부 노화 방지 및 개선을 위한 화장품으로 유용하게 이용될 수 있다.The seaweed ear fermentation extract of the present invention contains flavonoids and has an antioxidant effect. In addition, by inhibiting collagenase activity, which is a collagen-degrading enzyme, a composition containing it as an active ingredient can be usefully used as a cosmetic for preventing and improving skin aging.
미역귀추출물과 미역귀발효추출물의 항산화능력 비교Comparison of antioxidant capacity of seaweed ear extract and seaweed ear fermentation extract
이하에서 본 발명의 바람직한 실시형태를 실시예를 참고로 구체적으로 설명한다. 하지만 본 발명의 범위가 이러한 실시예에 한정되는 것은 아니다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to Examples. However, the scope of the present invention is not limited to these examples.
실시예 : 에탄올 추출물 분말 제조Example: Preparation of ethanol extract powder
건조된 미역귀시료(100g)와, 발효건조된 미역귀시료(100g) 3종에 각각 95% 에탄올 900mL를 첨가한 후, 배양기 37℃에서 24시간 씩 3회 추출하여 진공농축기를 이용하여 농축하고 동결건조하여 열수추출물과 발효추출물들의 건조시료를 얻었다.After adding 900 mL of 95% ethanol to each of the three dried seaweed ear samples (100g) and fermented seaweed ear samples (100g), extract three times at 37℃ for 24 hours in an incubator, concentrate using a vacuum concentrator, and freeze-drying. Thus, dry samples of the hot water extract and fermentation extract were obtained.
실험예Experimental example
총 플라보노이드 함량 측정Determination of total flavonoid content
각 시료추출액(열수추출물, 3종의 발효추출물)0.5 mL에 10% Aluminum Nitrate 0.5 mL와 1M potassium acetate 0.5 mL를 넣은 뒤, 80% 에탄올 2.0mL를 첨가하여 잘 혼합한 후 40분간 실온에 방치하여반응시킨 후 분광광도계를 이용하여 420nm에서 흡광도를 측정하였다. 이때, gallic acid을 이용하여 표준검량곡선을 작성하였고, 시료의 총 플라보노이드함량은 ml당 mg quercetin으로 나타내었으며, 실험은 3회 반복 수행하여 평균값으로 나타내었다.Add 0.5 mL of 10% Aluminum Nitrate and 0.5 mL of 1M potassium acetate to 0.5 mL of each sample extract (hot water extract, 3 fermentation extracts), add 2.0 mL of 80% ethanol, mix well, and leave at room temperature for 40 minutes. After reacting, absorbance was measured at 420 nm using a spectrophotometer. At this time, a standard calibration curve was prepared using gallic acid, and the total flavonoid content of the sample was expressed as mg quercetin per ml, and the experiment was repeated three times and expressed as an average value.
여기서, 열수추출물 수용액의 총 플라보노이드 함량을 정량한 결과 150.95mg/ml가 함유되었고, Lactobacillus planntarum는 177.69mg/ml, Lactobacillus breveis는 92.02mg/ml , Lactobacillus casie는 92.02mg/ml함유되어 있는 것을 확인하였고, 플라보노이드 함량은 Lactobacillus planntarum발효물이 열수추출물보다 1.17배 증가된 결과를 확인하였다.Here, as a result of quantifying the total flavonoid content of the hot water extract aqueous solution, it was confirmed that 150.95mg/ml was contained, Lactobacillus planntarum was 177.69mg/ml, Lactobacillus breveis was 92.02mg/ml, and Lactobacillus casie was 92.02mg/ml. , It was confirmed that the content of flavonoids in Lactobacillus planntarum fermented product was 1.17 times higher than that of hot water extract.
ABTS 양이온 라디칼 소거능ABTS cationic radical scavenging ability
7.4mM ABTS(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)와 2.7Mm potassium persulfate을 1:1로 섞어 하루 동안 암소에 방치하여 ABTS 양이온을 형성시킨 후, 이 용액을 735nm에서 흡광도 값이 0.7~1.0이 되도록 50% 메탄올로 희석해서 사용하였다. 열수추출물수용액과 발효추출물을 종류별로 동량 첨가한 후, 10분 반응시킨 후에 735nm에서 흡광도를 측정하였다. 결과 값은 시료를 첨가하지 않은 대조군과 비교하여 라디칼의 제거활성으로 나타냈다. 실험은 3회 반복 수행하여 평균값으로 나타내었다. 7.4mM ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) and 2.7Mm potassium persulfate are mixed 1:1 and left in the dark for one day to form ABTS cations, and then this solution was added to 735nm. It was diluted with 50% methanol so that the absorbance value was 0.7 to 1.0 in the hot-water extract aqueous solution and fermentation extract were added in equal amounts for each type, followed by reacting for 10 minutes, and the absorbance was measured at 735 nm. Compared to the control group, which was not used, it was expressed as radical scavenging activity, and the experiment was repeated three times and expressed as an average value.
ABTS 라디컬 제거 활성(%) = (1-A/B)x100ABTS radical scavenging activity (%) = (1-A/B)x100
A: 시료첨가구의 흡광도, B: 대조구의 흡광도A: absorbance of the sample addition, B: absorbance of the control
Claims (3)
A cosmetic composition characterized in that it improves anti-inflammatory and antioxidant efficacy, characterized by fermenting the seaweed ear hot water extract with lactic acid bacteria.
The cosmetic composition according to claim 1, wherein the composition contains a fermented product obtained by fermenting 0.001-10% by weight of seaweed extract hot water with Lactobacilus plantarum based on the total weight of the composition. .
The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition has a formulation selected from the group consisting of lotion (skin, lotion, essence), nourishing cream, massage cream, pack, oil, and nourishing essence.
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CN114672519A (en) * | 2022-02-24 | 2022-06-28 | 广州市巧美化妆品有限公司 | Thallus laminariae/hot spring water fermentation broth, product containing thallus laminariae/hot spring water fermentation broth, and preparation method and application of thallus laminariae/hot spring water fermentation broth |
CN114672519B (en) * | 2022-02-24 | 2022-11-15 | 广州市巧美化妆品有限公司 | Undaria pinnatifida/hot spring water fermentation liquor, product containing same, and preparation method and application thereof |
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