KR20120139003A - An antibacterial and antioxidant composition using sedum extract - Google Patents
An antibacterial and antioxidant composition using sedum extract Download PDFInfo
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- KR20120139003A KR20120139003A KR1020110058500A KR20110058500A KR20120139003A KR 20120139003 A KR20120139003 A KR 20120139003A KR 1020110058500 A KR1020110058500 A KR 1020110058500A KR 20110058500 A KR20110058500 A KR 20110058500A KR 20120139003 A KR20120139003 A KR 20120139003A
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Abstract
Description
본 발명은 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물에 관한 것으로, 더 상세하게는 기린초류를 충분히 건조시킨 다음 용매와의 접촉면을 높이기 위하여 분쇄시킨 후, 상기 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치하여 기린초류 추출물을 제조하는 것이다.The present invention relates to an antimicrobial and antioxidant composition comprising the giraffe herb extract as an active ingredient, and more specifically, after the giraffe herb is sufficiently dried, pulverized to increase the contact surface with the solvent, the solvent is mixed with the giraffe herb. By standing at room temperature for 1 to 2 days to prepare a giraffe herb extract.
본 발명에서는 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물의 항균 기능, 항산화 활성, Nitrite 소거능으로 인해 항균제 및 항산화제로서 기능을 충분히 할 수 있으며, 천연 기능성 소재로서 식품 분야의 항균보존제로서의 응용이 가능하고, 천연의 항균성을 지닌 화장품으로서의 응용 가능성과 의약품 및 건강기능식품, 사료첨가제 분야에서도 새로운 소재로서 활용이 가능하다.
In the present invention, due to the antimicrobial function, antioxidant activity, and Nitrite scavenging ability of the antimicrobial and antioxidant composition using the giraffe herb extract as an active ingredient, it can fully function as an antimicrobial agent and an antioxidant, and its application as an antimicrobial preservative in the food field as a natural functional material. It is possible to apply it as a new material in the field of application as a cosmetic with natural antimicrobial properties and in the fields of medicines, health foods and feed additives.
기린초류는 쌍떡잎식물 장미목 돌나무과에 속하는 식물체로서 그 종류로는 기린초(Sedum kamtschaticum Fischer.), 가는기린초(Sedum aizoon), 넓은잎기린초(Sedum japonicum/Sedum ellacombianum Praeger.), 섬기린초(Sedum takesimense Nakai.), 속리기린초(Sedum zokuriense Nakai.), 애기기린초(Sedum middendroffianum Max.), 큰기린초(Sedum aizoon var. heterodontum), 태백기린초(Sedum latiovalifolium Y. Lee)등이 있다.Sedum kamtschaticum flow is a plant belonging to Araliaceae The dicotyledonous plant rosewood stone sorters are Sedum kamtschaticum (Sedum kamtschaticum Fischer.), Sedum aizoon ), broadleaf giraffe ( Sedum japonicum / Sedum ellacombianum Praeger.), island giraffe ( Sedum takesimense Nakai.), creepy giraffe ( Sedum zokuriense Nakai.), Agirincho ( Sedum middendroffianum Max.), Great Giraffe ( Sedum aizoon var. heterodontum), Taebaek Giraffes ( Sedum latiovalifolium Y. Lee).
기린초(Sedum Kamtschaticum Fisch)는 한국(경기, 함남), 일본, 사할린, 쿠릴, 캄차카, 아무르, 중국 등에서 산지의 바위 곁에 서식하는 5~30cm 높이의 다년생 초본으로서, 뿌리줄기는 매우 굵고 원줄기의 한군데에서 줄기가 뭉쳐나며 원기둥 모양이다. 잎은 어긋나고 거꾸로 선 달걀 모양 또는 긴 타원 모양으로 톱니가 있으며 잎자루는 거의 없고 육질(肉質)이다. 6~7월에 노란꽃이 취산꽃차례로 꼭대기에 많이 핀다. 꽃잎은 바소꼴로 5개이며 끝이 뾰족하다. 꽃받침은 바소꼴의 줄 모양으로 5개이며 녹색이다. 수술은 10개이고 암술은 5개 이다. 전초에서 켐페롤, 구에르쩨틴, 미리쩨틴, 아르부틴, 몰식자산탄닌이 확인 되었으며, 한방에서는 기린초, 가는기린초(Sedum arizoon L.) 및 속리기린초(Sedum zokuriense Nakai)의 전초를 비채라 하여 활혈, 지혈, 이수, 진정, 소종의 용도로 사용하고 있다. 어린순은 삶아 쓴맛을 제거한 후 나물을 해서 먹는다. Sedum kamtschaticum (Sedum Kamtschaticum Fisch) is a perennial herb that is 5 to 30 cm tall, inhabited by rocks in the mountains in Korea (Gyeonggi, Hamnam), Japan, Sakhalin, Kuril, Kamchatka, Amur, China, etc. It is cylindrical in shape. Leaves are alternate, inverted egg-shaped or long oval, serrated, with few petioles, fleshy. In June-July, yellow flowers bloom in bloom at the top. Petals are 5 small, and have sharp tips. Calyxes are 5 rows of green, lanceolate. There are 10 surgeries and 5 pistils. Chem outpost in Ferrol, Puerto jjetin old, pre jjetin, arbutin, tannin, gallic acid has been identified, the Oriental Sedum kamtschaticum, go Sedum kamtschaticum (Sedum arizoon L. and Sedum zokuriense Nakai) is used as a non-chaebol for the use of blood, hemostasis, diarrhea, sedation, and seedlings. Young sprouts are boiled and removed after bitterness.
상기 기린초류들은 주로 재배 및 식생(원예과학기술지 28권 3호 pp. 394-402, 화훼연구 16권 3호 pp. 205-210), 생약학적 연구(생약학회지 39권 2호 pp. 137-141 2008), 살선충효과(한국응용곤충학회지 45권 3호 pp. 339-346 2006, 한국응용곤충학회지 45권 3호 pp. 347-355 2006), 아폽토시스 저해 활성(특허 제 10-0571582호), 항염증용 약제학적 조성물(특허 제10-0470158)에 대해서는 알려져 있으나 항산화 및 항균 기능은 알려진 바가 없다. The giraffe herb is mainly cultivated and vegetation (Horticultural Science and Technology Journal No. 28, No. 3 pp. 394-402, Flower Research No. 16, No. 3 pp. 205-210), Medicinal research (Korean Journal of Pharmacognosy No. 39, no. 141 2008), Insecticidal Effect (Korean Society of Applied Entomology, Vol. 45, No. 3 pp. 339-346 2006, Korean Society of Applied Entomology, Vol. 45, No. 3, pp. 347-355 2006), Apoptosis inhibitory activity (Patent No. 10-0571582) , Anti-inflammatory pharmaceutical composition (Patent No. 10-0470158) is known, but the antioxidant and antibacterial function is not known.
화장품 산업에서 오염을 일으키는 미생물들은 슈도모나스 속(Pseucomonas spp.), 세라치아 속(Serratia spp.), 에스케리치아 속(Escherichia spp.), 엔테로박터 속(Enterobacter spp.), 클렙시엘라 속(Klebsiella spp.), 프로테우스 속(Proteus spp.), 스타필로코커스 속(Staphylococcus spp.), 스트렙토코커스 속(Streptococcus spp.), 바실러스 속(Bacillus spp.), 클로스트리디움 속(Clostridium spp.), 캔디다 알비칸스(Candida albicans) 등이 있다(화장품과 미생물, 2005, p72-79). Contaminating microorganisms in the cosmetic industry include Pseucomonas spp., Serratia spp., Escherichia spp., Enterobacter spp. And Klebsiella. spp.), Proteus spp., Staphylococcus spp., Streptococcus spp., Bacillus spp., Clostridium spp., Candida albicans (Candida albicans ) (cosmetics and microorganisms, 2005, p72-79).
상기 미생물들에 의한 부패를 방지하고 제품 안정성을 높이기 위해서 식품 및 화장품, 건강기능식품, 의약품에는 방부제가 필수적이다. 제품 특성상 유통기간이 비교적 길며 미생물 영양원이 많은 화장품의 경우는 더욱 그러하다. 그러나 기존의 방부제로서 화장품, 의약품에 범용적으로 사용되는 파라벤류의 방부제들 조차 피부알러지(Andrea Counti 등, Contact Dermatitis, 1997, 37;35-36)와 환경호르몬으로서의 가능성(Edwin 등, Toxicology and Applied Pharmacology, 1998, 153;12-19) 및 내성균 유발이라는 문제점을 가지고 있다. 뿐만 아니라 식품용 방부제들은 허용된 기준내의 사용도 불신되고 있고 지속적인 체내 축적으로 인한 급?만성 독성, 돌연변이 유발 등의 새로운 문제 가능성이 대두되고 있다(신동화, 식품과학과 산업, 1990, 23(4) 68-72). In order to prevent corruption by the microorganisms and increase product stability, preservatives are essential in foods, cosmetics, health functional foods, and medicines. Due to the nature of the product, the shelf life is relatively long and even more so for cosmetic products with many microbial nutrients. However, even parabens preservatives commonly used in cosmetics and medicines as existing preservatives are known as skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37; 35-36) and their potential as environmental hormones (Edwin et al., Toxicology and Applied). Pharmacology, 1998, 153; 12-19) and resistant bacteria. In addition, food preservatives are distrusted in the use of accepted standards, and new problems such as acute and chronic toxicity and mutagenesis due to continuous body accumulation are emerging (Xinhua, Food Science and Industry, 1990, 23 (4) 68). -72).
이러한 문제점들로 인하여 제품의 안전성과 경제성이 우수한 천연방부제에 대한 연구가 지속되고 있으며 그 결과로 향신류, 정유, 한약재 등 동?식물기원의 추출물(Kim 등, Kor. J. Food Sci. Technol. 35(2): 159-165(2003); Dong 등, J. Kor. Soc. Food. Sci. Nutr. 33(4): 609-613(2004); Sebastien, F. 등, Molecular Immunology, 40:395-405(2003); Losso, J. N. 등, Naidu. A. S. (ed.). P. 17-102(2000); Sagoo, S. 등, Food Microbiol. 19: 175-182 (2002))과 박테리오신 같은 미생물 기원의 천연항균성물질이 보고되고 있다(Hansen, J. N., Academic Press, Inc., San Diego, USA. P. 93-120 (1993); Kito, M. 등, FEMS Micobiol Lett. 207: 147-151 (2002); Cho 등, Food Science and Industry (Vol. 38, No. 2) pp. 36-45). Due to these problems, research on natural preservatives with excellent safety and economical efficiency of products is ongoing, and as a result, extracts of animal and vegetable origins such as spices, essential oils, and herbal medicines (Kim et al., Kor. J. Food Sci. Technol. 35 (2): 159-165 (2003); Dong et al., J. Kor. Soc. Food.Sci.Nutr. 33 (4): 609-613 (2004); Sebastien, F. et al., Molecular Immunology, 40: 395-405 (2003); Losso, JN et al., Naidu. AS (ed.), P. 17-102 (2000); Sagoo, S. et al., Food Microbiol. 19: 175-182 (2002)) and bacteriocins Natural antimicrobial agents of microbial origin have been reported (Hansen, JN, Academic Press, Inc., San Diego, USA.P. 93-120 (1993); Kito, M. et al., FEMS Micobiol Lett. 207: 147-151 (2002); Cho et al., Food Science and Industry (Vol. 38, No. 2) pp. 36-45).
천연의 항균성 물질로 알칼로이드(alkaloid), 후라보노이드(flavonoid), 피토알렉신(phytoalexin), 항균 펩타이드 등이 알려져 있으며, 유기산과 지방산 등의 항균성에 대해서도 알려져 있다. 이들 대부분은 산의 pH에 의한 효과와 킬레이트에 의한 효과가 주 메커니즘일 것으로 추정된다(E1-shenawa, MA 등, J.Food Protec. 1989, 52(11):771-778)(Bizri, JN등, J.Food Science, 1994,59(1), 130-135). 그러나 보고된 천연항균성 물질의 대부분은 색취, pH 저하, 좁은 항균 스펙트럼, 제형상의 문제점 등으로 인하여 상용화되지 못하고 있으며, 편백 추출물인 히노키티올(Hinokitiol), 목련 추출물인 메그노놀(Megnonol), 자몽종자추출물, 복합황금추출물 등 일부만 상용화되고 있다. Alkaloids (alkaloids), flavonoids (phytoalexin), antimicrobial peptides and the like are known as natural antimicrobial substances, and antimicrobial agents such as organic acids and fatty acids are also known. Most of them are thought to be the main mechanism of acid pH effect and chelate effect (E1-shenawa, MA et al., J. Food Protec. 1989, 52 (11): 771-778) (Bizri, JN, etc.). , J. Food Science, 1994, 59 (1), 130-135). However, most of the reported natural antimicrobial substances have not been commercialized due to color odor, pH decrease, narrow antibacterial spectrum, and formulation problems. Only some of the extracts and complex golden extracts are commercialized.
활성 산소에 대한 항산화 물질의 작용기전은 크게 3가지로 나뉠 수 있다. 예방적인 측면, 활성산소를 제거하는 측면 그리고 조직회복 및 신생에 관여하는 기능으로 분류할 수 있다. 이 중 식물유래 항산화 물질의 작용기전은 주로 활성산소를 제거하는 항산화 물질로서의 기능을 담당한다. 즉 반응성이 크고 유해한 활성산소와 같은 유리기와 먼저 반응하여 자기 자신은 안정성이 있는 유리기로 되어 다른 중요한 화합물이 유리기가 되는 연쇄반응을 막아준다. 생체 내에서는 예방적 항산화 물질의 예를 들면 SOD와 같은 효소작용에 의해 활성산소와의 반응은 어느 정도 억제되지만, 여전히 생체 내에서는 소량의 활성산소가 생성되며 외부에서도 활성산소(대기오염, 담배연기 등)가 체내로 들어오기도 한다. 항산화 물질은 동, 식물계에 널리 분포되어 있는데, 과일과 채소에 많은 페놀성 화합물, 플라보노이드, 토코페롤, 비타민 C, 셀레늄과 같은 항산화물질은 지방의 산화를 지연시키거나 방지하며, 암, 심장혈관계 질환 등을 예방, 지연시킴으로써 노화방지에도 중요한 역할을 한다. 식물계에 존재하는 천연 항산화물질인 토코페롤과 비타민 C는 식품, 의약품 및 화장품 등에 널리 이용되고 있다. 유지 또는 유지함유 식품의 산패는 주로 공기 중의 산소와 결합에 의해 일어나는데, 이를 방지하기 위하여 많은 합성 또는 천연 항산화 물질이 개발되어 왔으나, 그 효과와 경제성 때문에 실제로 많이 사용되고 있는 것은 합성 항산화제이다. 합성 항산화제로는 BHT(Butylated Hydroxy Toluene), BHA(Butylated Hydroxy Anisole), Propyl Gallate 등이 있다. 합성 항산화제는 항산화력이 뛰어나고 가격이 저렴하여 상업용 식품에 많이 사용되고 있으나, 인체 부작용 등 안전성에 대한 우려로 그 사용량이 법적으로 규제되어 있다. 토코페롤과 같은 천연 항산화제는 인체에 대하여 안전하기는 하나 단독으로는 산화 연쇄반응 저지 능력이 낮고, 가격이 비싼 단점이 있다. 따라서, 안전성이 뛰어나면서도 경제성이 있는 항산화제 개발이 지속적으로 요구되고 있다. There are three major mechanisms of action of antioxidants on free radicals. It can be classified into preventive aspects, removal of free radicals, and functions involved in tissue recovery and angiogenesis. Of these, the mechanism of action of plant-derived antioxidants is primarily responsible for the function of antioxidants to remove free radicals. In other words, by reacting free radicals such as reactive and harmful free radicals first, they become stable free radicals to prevent chain reactions in which other important compounds are free radicals. In vivo, the reaction of prophylactic antioxidants, such as SOD, to some extent inhibits the reaction with free radicals, but a small amount of free radicals are still produced in vivo and free radicals (air pollution, tobacco smoke) Etc.) may enter the body. Antioxidants are widely distributed in the copper and plant systems. Antioxidants such as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium in fruits and vegetables delay or prevent the oxidation of fats, and cancer, cardiovascular diseases, etc. By preventing and delaying, it plays an important role in preventing aging. Tocopherols and vitamin C, which are natural antioxidants present in the plant kingdom, are widely used in food, medicine, and cosmetics. The rancidity of fats and oils or foods containing oils and fats is mainly caused by the combination with oxygen in the air. To prevent this, many synthetic or natural antioxidants have been developed. Synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are used in commercial foods because of their excellent antioxidant power and low price, but their usage is legally regulated due to concerns about safety such as human side effects. Natural antioxidants, such as tocopherol, are safe for the human body, but have low disadvantages in preventing oxidative chain reactions and are expensive. Therefore, there is a continuous need for development of antioxidants with excellent safety and economical efficiency.
기린초 또는 섬기린초 추출물에 관한 종래기술로는 물을 추출용매로 하여 추출한 기린초 추출물과 송이추출물을 함유하는 미백화장료 조성물(등록특허 제 10-0483338호), 유기용매를 추출용매로 하여 추출한 기린초 추출물로부터 분리한 화합물 및 이들을 유효성분으로 함유하는 항염증용 약제학적 조성물(등록특허 제 10-0470158호), 섬기린초로부터 분리한 아폽토시스 저해 활성을 가지는 화합물 및 이의 분리방법(등록특허 제 10-0571582호) 등의 기린초 또는 섬기린초 추출물에 관련된 기술들이 있었으나, 항산화 및 항균 기능을 갖는 기린초류 추출물에 대한 종래기술은 없는 실정이다.
Conventional techniques relating to giraffe or giraffe-cho extracts include giraffe-cho extract extracted with water as an extraction solvent, whitening cosmetic composition containing pine extract (Registration No. 10-0483338), and giraffe-cho extract extracted with an organic solvent as an extraction solvent. Isolated compounds and anti-inflammatory pharmaceutical compositions containing them as an active ingredient (Patent No. 10-0470158), compounds having apoptosis inhibitory activity isolated from Seogirincho and its separation method (Patent No. 10-0571582) Although there have been techniques related to giraffe herb or island giraffe herb extracts, etc., there is no prior art for the giraffe herb extract having antioxidant and antibacterial functions.
본 발명은 상기와 같은 문제를 해결하고자 발명한 것으로, 그 목적은 기린초류를 충분히 건조시킨 다음 용매와의 접촉면을 높이기 위하여 분쇄시킨 후, 상기 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치하여 제조된 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물을 제조하여 안전성이 뛰어나면서도 경제성이 있는 천연의 기능성 물질로서의 항균 및 항산화 조성물을 제공함에 있다.
The present invention has been invented to solve the above problems, the object of which is to dry the giraffe herb sufficiently and then pulverized to increase the contact surface with the solvent, by mixing the solvent in the pulverized giraffe herb 1 to 2 at room temperature The present invention provides an antimicrobial and antioxidant composition as a natural functional substance having excellent safety and economical efficiency by preparing an antimicrobial and antioxidant composition comprising the giraffe herb extract prepared by standing for one day.
상기와 같은 목적을 달성하기 위하여 본 발명에 따른 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물은 스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa), 에스케리치아 콜리(Escherichia coli), 캔디다 알비칸스(Candida albicans), 아스퍼질러스 나이거(Aspergillus niger)에 항균력이 있는 것을 특징으로 한다.In order to achieve the above object, the antimicrobial and antioxidant composition using the giraffe herb extract according to the present invention as an active ingredient is Staphylococcus aureus ( Staphylococcus). aureus ), Pseudomonas aeruginosa ), Escherichia coli), Candida albicans (Candida albicans ), Aspergillus niger ( Apergillus niger ) is characterized by having an antimicrobial activity.
또한, 상기 기린초류 추출물은 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치하여 제조하거나, 분쇄된 기린초류 추출물에 용매를 혼합하여 상온에서 1~2일간 정치하여 제조된 기린초류 추출물에 에틸아세테이트를 더 가한 후 에틸아세테이트층을 수거하여 제조하는 것을 특징으로 한다. In addition, the giraffe herb extract is prepared by mixing the solvent in the pulverized giraffe vinegar to stand at room temperature for 1 to 2 days, or by mixing the solvent in the pulverized giraffe vinegar extract to stand at room temperature for 1 to 2 days After further adding ethyl acetate to the ethyl acetate layer is characterized in that the production.
또한, 상기 기린초류 추출물은 물, C1~C4 알코올, 아세톤, 핵산, 에틸아세테이트, 이산화탄소 중 하나 또는 둘 이상을 혼합한 것을 사용한 용매추출 또는 분획에 의해 제조하는 것을 특징으로 한다. In addition, the giraffe herb extract is characterized in that it is prepared by solvent extraction or fractionation using one or two or more of water, C1 ~ C4 alcohol, acetone, nucleic acid, ethyl acetate, carbon dioxide.
또한, 상기 항균 및 항산화 조성물은 식품첨가물, 화장품 조성물, 건강기능성 식품, 사료첨가제, 치료용 약제 조성물 등으로 활용되는 것을 특징으로 한다.
In addition, the antimicrobial and antioxidant composition is characterized in that it is utilized as food additives, cosmetic compositions, health functional foods, feed additives, therapeutic pharmaceutical compositions and the like.
본 발명에 따른 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물은 항균 기능, 항산화 활성, Nitrite 소거능으로 인해 새로운 항균제 및 항산화제로서 기능을 충분히 할 수 있으며, 안전성이 뛰어나면서도 경제성이 있는 천연 기능성 소재로서 식품 분야의 항균보존제로서의 응용이 가능하고, 천연의 항균성을 지닌 화장품으로서의 응용 가능성과 의약품 및 건강기능식품, 사료첨가제 분야에서도 새로운 소재로서 활용이 가능하다.
The antimicrobial and antioxidant composition comprising the giraffe herb extract according to the present invention as an active ingredient can fully function as a new antimicrobial and antioxidant due to its antibacterial function, antioxidant activity, and nitrite scavenging ability, and is a natural functional material with excellent safety and economical efficiency. As an antimicrobial preservative in the food field, it is possible to apply it as a new material in the fields of application as a cosmetic with natural antimicrobial properties and in the fields of medicines, health foods and feed additives.
도 1은 시간경과에 따른 세균 수 측정결과 도이다.
도 2는 시간경과에 따른 진균 수 측정결과 도이다.
1 is a view showing the number of bacteria measured over time.
2 is a view showing the results of the fungal fungus over time.
본 발명은 기린초류 추출물을 유효성분으로 하는 항균 및 항산화 조성물에 관한 것으로, 기린초류를 충분히 건조시킨 다음 용매와의 접촉면을 높이기 위하여 분쇄시킨 후, 상기 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치하여 기린초류 추출물을 제조하는 것을 특징으로 한다. The present invention relates to an antimicrobial and antioxidant composition comprising the giraffe herb extract as an active ingredient, and after drying the giraffe herb sufficiently to pulverize to increase the contact surface with the solvent, the solvent is mixed with the pulverized giraffe herb at room temperature. It is characterized by producing a giraffe herb extract by standing for ~ 2 days.
또 다른 방법으로는, 기린초류를 충분히 건조시킨 다음 용매와의 접촉면을 높이기 위하여 분쇄시킨 후, 상기 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치한 후, 이를 여과시켜 잔여 불순물을 분리시킨 후 감압 농축시켜 용매추출물을 제조하고, 상기 용매추출물을 건조한 후에 물에 재용해하고, 상기 재용해된 용매추출물에 에틸아세테이트를 가한 후 에틸아세테이트층을 수거하여 기린초류 추출물을 제조할 수 있다. In another method, the giraffe herb is sufficiently dried and then pulverized to increase the contact surface with the solvent. The giraffe herb is mixed with a solvent and left to stand at room temperature for 1 to 2 days, and then filtered to remove residual impurities. After separation, the mixture is concentrated under reduced pressure to prepare a solvent extract, the solvent extract is dried and redissolved in water, ethyl acetate is added to the redissolved solvent extract, and the ethyl acetate layer is collected to prepare a giraffe herb extract. .
상기 기린초류는 쌍떡잎식물 장미목 돌나무과에 속하는 식물체로서 그 종류로는 기린초, 가는기린초, 넓은잎기린초, 섬기린초, 속리기린초, 애기기린초, 큰기린초, 태백기린초 등이 있다. The giraffe herb is a plant belonging to the dicotyledonous rosewood family, and the kinds thereof include giraffe, fine giraffe, broad-leaf giraffe, island giraffe, creeper giraffe, agigi-lin, big giraffe, baekbaek giraffe and the like.
그리고, 상기 기린초류를 충분히 건조시킨 다음 용매와의 접촉면을 높이기 위하여 분쇄시킨 후, 상기 분쇄된 기린초류에 용매를 혼합하여 추출할 때, 상기 용매는 물, C1~C4 알코올, 아세톤, 핵산, 에틸아세테이트, 이산화탄소 중 하나 또는 둘 이상을 혼합한 것을 사용하며, 기린초류의 유효성분이 파괴되지 않거나 최소화된 조건에서 실온 또는 가온하여 추출할 수 있고, 추출 방법은 특별히 제한되지 않으며, 바람직하게는 냉침 추출, 초음파 추출, 환류 냉각 추출, 열수추출, 초임계 추출을 할 수 있다. Then, the giraffe herb is sufficiently dried and then pulverized to increase the contact surface with the solvent, and when the solvent is extracted by mixing the pulverized giraffe vinegar, the solvent is water, C1-C4 alcohol, acetone, nucleic acid, ethyl One or a mixture of two or more of acetate and carbon dioxide may be used, and the active ingredient of the giraffe herb may be extracted at room temperature or warmed under conditions that are not destroyed or minimized, and the extraction method is not particularly limited. Ultrasonic extraction, reflux cooling extraction, hot water extraction, supercritical extraction can be performed.
그리고, 상기 분쇄된 기린초류에 용매를 혼합하여 추출할 때, 상기 분쇄된 기린초류에 용매를 중량대비 1 : 10~100의 비율로 혼합하여 1시간~7일, 바람직하게는 1~2일 동안 정치하여 추출물을 얻는 것이다. In addition, when the solvent is mixed with the pulverized giraffe herb and extracted, the pulverized giraffe is mixed with a solvent in a ratio of 1: 10 to 100 by weight to 1 hour to 7 days, preferably 1 to 2 days. It is left to stand to obtain an extract.
그리고, 상기 기린초류 추출물은 항균 효과가 있으며, 특히, 스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa), 에스케리치아 콜리(Escherichia coli), 캔디다 알비칸스(Candida albicans), 아스퍼질러스 나이거(Aspergillus niger)에 항균력이 있는 것을 특징으로 한다.
In addition, the giraffe herb extract has an antibacterial effect, in particular, Staphylococcus aureus ( Staphylococcus aureus ), Pseudomonas aeruginosa , Escherichia coli), Candida albicans (Candida albicans ), Aspergillus niger ) is characterized by having an antimicrobial activity.
본 발명의 기린초류 추출물은 항산화 활성 및 미생물에 대한 선택적인 생육억제효과가 뛰어남으로, 이를 함유하는 조성물은 산화 및 세균에 의한 감염증의 치료, 예방 또는 완화시킬 수 있는 식품 및 화장품, 건강기능식품, 의약품 및 동물사료의 활성성분으로 개발될 수 있다.
Since the giraffe herb extract of the present invention is excellent in antioxidant activity and selective growth inhibitory effect on microorganisms, the composition containing the same foods and cosmetics, health functional foods, which can cure, prevent or alleviate the infection caused by oxidation and bacteria, It can be developed as an active ingredient in medicine and animal feed.
이하에서 실시예를 통하여 본 발명을 보다 구체적으로 설명한다. 그러나 하기의 실시예는 본 발명을 구체적으로 예시하기 위한 것일 뿐, 본 발명의 권리범위를 제한하는 것이 아님은 당업자에게 있어서 명백한 사실이다. 즉, 본 발명의 단순한 변형 내지 변경은 당업자에 의하여 용이하게 실시될 수 있으며, 이러한 변형이나 변경은 모두 본 발명의 영역에 포함되는 것으로 볼 수 있다.
Hereinafter, the present invention will be described more specifically by way of examples. It will be apparent to those skilled in the art, however, that the following examples are illustrative of the present invention only and do not limit the scope of the present invention. That is, a simple modification or change of the present invention can be easily performed by those skilled in the art, and all such modifications and alterations can be considered to be included in the scope of the present invention.
실시예 1: 기린초 추출물 제조Example 1: giraffe extract
추출에 사용되는 기린초류는 우리나라의 산야에 자생하는 것으로 잎과 줄기를 포함하고 있는 전초를 사용하였다. The giraffe herb used for extraction is native to Korea's hills and uses outposts containing leaves and stems.
기린초 100 g에 메탄올(MeOH) 900 ml을 가하여 실온에서 1일 또는 2일간 정치하여 추출물 얻었다. 이 여액을 여과지(Whatman filter paper No. 2)로 여과하여 회전식 진공 농축기로 감압 농축하여 기린초 MeOH 추출물 5.6 g을 얻었다.
900 ml of methanol (MeOH) was added to 100 g of giraffe, and the extract was left to stand at room temperature for 1 or 2 days. The filtrate was filtered using a filter paper (Whatman filter paper No. 2), and concentrated under reduced pressure with a rotary vacuum concentrator to obtain 5.6 g of Kirincho MeOH extract.
실시예 2: 기린초 에틸아세테이트 분획물 제조 Example 2 Preparation of Kirincho Ethyl Acetate Fraction
상기 실시예 1에서 얻어진 시료에 100 ml H2O에 재용해시키고 여기에 100 ml 에틸아세테이트(ehylacetate)를 첨가하여 ethlyacetate, 수용성 분획물을 얻고, ethylacetate 분획물을 여과지로 여과하여 회전식 진공 농축기로 감압, 농축하였으며 건조된 분획물 0.467 g을 얻었으며, 이를 하기의 실험 시료로 사용하였다.
The sample obtained in Example 1 was redissolved in 100 ml H 2 O and 100 ml ethylacetate was added thereto to obtain ethlyacetate, an aqueous fraction, and the ethylacetate fraction was filtered through a filter paper and decompressed and concentrated with a rotary vacuum concentrator. 0.467 g of dried fractions were obtained, which were used as the following experimental samples.
실시예 3: 섬기린초 추출물 제조 Example 3: Preparation of Seogirinchocho Extract
섬기린초 100 g에 메탄올(MeOH) 900 ml을 가하여 실온에서 1일 또는 2일간 정치하여 추출물 얻었다. 이 여액을 여과지(Whatman filter paper No. 2)로 여과하여 회전식 진공 농축기로 감압 농축하여 섬기린초 MeOH 추출물 1.9g을 얻었다.
900 ml of methanol (MeOH) was added to 100 g of sumirincho, and left to stand at room temperature for 1 or 2 days to obtain an extract. The filtrate was filtered through a filter paper (Whatman filter paper No. 2), and concentrated under reduced pressure with a rotary vacuum concentrator to obtain 1.9 g of Seogirincho MeOH extract.
실시예 4: 섬기린초 에틸아세테이트 분획물 제조Example 4 Preparation of Seogirincho Ethyl Acetate Fraction
상기 실시예 3에서 얻어진 시료에 100 ml H2O에 재용해시키고 여기에 100 ml 에틸아세테이트(ethylacetate)를 첨가하여 ethlyacetate, 수용성 분획물을 얻고, ethylacetate 분획물을 여과지로 여과하여 회전식 진공 농축기로 감압, 농축하였으며 건조된 분획물 0.3g을 얻었으며, 이를 하기의 실험 시료로 사용하였다.
The sample obtained in Example 3 was redissolved in 100 ml H 2 O, and 100 ml ethylacetate was added thereto to obtain ethlyacetate, an aqueous fraction, and the ethylacetate fraction was filtered through a filter paper and decompressed and concentrated with a rotary vacuum concentrator. 0.3 g of dried fractions were obtained, which were used as the following experimental samples.
실시예 5: 가는기린초 추출물 제조Example 5: Preparation of Giraffe Extract
가는기린초 100 g에 메탄올(MeOH) 900 ml을 가하여 실온에서 1일 또는 2일간 정치하여 추출물 얻었다. 이 여액을 여과지(Whatman filter paper No. 2)로 여과하여 회전식 진공 농축기로 감압 농축하여 가는기린초 MeOH 추출물 2.21g을 얻었다.
900 ml of methanol (MeOH) was added to 100 g of fine giraffe, and the extract was left to stand at room temperature for 1 or 2 days. The filtrate was filtered using a filter paper (Whatman filter paper No. 2), and concentrated under reduced pressure with a rotary vacuum concentrator to obtain 2.21 g of fine Kirincho MeOH extract.
실시예 6: 가는기린초 에틸아세테이트 분획물 제조Example 6 Preparation of Fine Kirincho Ethyl Acetate Fraction
실시예 5에서 얻어진 시료에 100 ml H2O에 재용해시키고 여기에 100 ml 에틸아세테이트(ethylacetate)를 첨가하여 ethlyacetate, 수용성 분획물을 얻고, ethylacetate 분획물을 여과지로 여과하여 회전식 진공 농축기로 감압, 농축하였으며 건조된 분획물 0.105g을 얻었으며, 이를 하기의 실험 시료로 사용하였다.
The sample obtained in Example 5 was redissolved in 100 ml H 2 O and 100 ml ethylacetate was added thereto to obtain ethlyacetate, an aqueous fraction, and the ethylacetate fraction was filtered through a filter paper and decompressed and concentrated with a rotary vacuum concentrator. 0.105 g of dried fractions were obtained, which were used as the following experimental sample.
실험 1: 항균력 평가Experiment 1: Antimicrobial Evaluation
상기 실시예 1 내지 6에서 얻어진 시료에 대하여 세균 3종과 진균 2종에 대한 항균력을 평가하였다. 실험에 사용한 미생물 균주는 스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa) 및 에스케리치아 콜리(Escherichia coli), 캔디다 알비칸스(Candida albicans) 및 아스퍼질러스 나이거(Aspergillus niger) 였다.The antimicrobial activity against three bacteria and two fungi was evaluated for the samples obtained in Examples 1 to 6. The microbial strain used in the experiment was Staphylococcus aureus ), Pseudomonas aeruginosa ) and Escherichia coli), Candida albicans (Candida albicans ) and Aspergillus niger niger ).
스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa) 및 에스케리치아 콜리(Escherichia coli)는 Tryptic soy agar(TSA, pancreatic digest of casein 15g, papaic digest of soybean 5g, sodium chloride 5g, agar 15g)에서 37℃, 24시간 전배양 하고 이를 105-6 CFU/ml 농도가 되도록 희석하여 접종 균액으로 준비하였다. Staphylococcus aureus ), Pseudomonas aeruginosa ) and Escherichia coli ) was pre-incubated at Tryptic soy agar (TSA, pancreatic digest of casein 15g, papaic digest of soybean 5g, sodium chloride 5g, agar 15g) at 37 ° C for 24 hours and diluted to 10 5-6 CFU / ml. Prepared with inoculum bacterial solution.
캔디다 알비칸스(Candida albicans), 아스퍼질러스 나이거(Aspergillus niger)는 Potato dextrose agar(PDA, potatoe starch 4g, dextrose 20g, agar 15g)에서 30℃, 48시간 전배양 하여 105-6 CFU/ml의 접종액을 준비하였다.Candida albicans (Candida albicans ), Aspergillus niger prepared 10 5-6 CFU / ml inoculum at 30 ° C. for 48 hours in Potato dextrose agar (PDA, potatoe starch 4g, dextrose 20g, agar 15g) It was.
세균은 Tryptic soy broth(TSB, pancreatic digest of casein 17g, papaic digest of soybean 13g, dextrose 2.5g, sodium chloride 5g, dipotassium phosphate 2.5g)을, 진균은 Potato dextrose broth(PDB, potatoe starch 4g, dextrose 20g)을 액체배지로 사용하였다.Bacteria were Tryptic soy broth (TSB, pancreatic digest of casein 17g, papaic digest of soybean 13g, dextrose 2.5g, sodium chloride 5g, dipotassium phosphate 2.5g), and fungi were Potato dextrose broth (PDB, potatoe starch 4g, dextrose 20g). Was used as the liquid medium.
위와 같이 제조하여 멸균한 액체 배지를 96 well plate의 각 well에 100㎕씩 넣고(첫 번째, 두 번째 well 제외), 상기 실시예 1 내지 6에서 얻어진 시료를 96 well plate의 첫 번째와 두 번째 well에 각각 100㎕씩 넣은 후, 두 번째 well부터 Two-fold dilution method로 serial dilution을 진행하였다. 그 다음 각 well에 들어 있는 시료 희석액에 균을 접종한 후 세균은 37℃ 배양기에서 24시간, 진균은 30℃ 배양기에서 48시간 배양하였다. 배양 후 육안으로 관찰해서 항균 물질의 최소생육저해농도(Minimal Inhibitory Concentration, MIC)를 측정하였다. 그 결과를 하기 아래 표 1에 나타내었다(단위: %).100 μl of the liquid medium prepared and sterilized as described above into each well of the 96 well plate (excluding the first and second wells), and the samples obtained in Examples 1 to 6 were used in the first and second wells of the 96 well plate. Into each 100μL, serial dilution was performed by a two-fold dilution method from the second well. Then, after inoculating the bacteria in the sample diluent in each well, bacteria were incubated for 24 hours in a 37 ℃ incubator, 48 hours in a 30 ℃ incubator. After cultivation, visual observation was performed to determine the minimum inhibitory concentration (MIC) of the antimicrobial substance. The results are shown in Table 1 below (unit:%).
검정균
Test bacteria
에어루지노사
(Pseudomonas
aerugionsa)Pseudomonas
Air Ruginosa
( Pseudomonas
aerugionsa )
( Escherichia coli)Escherichia coli
( Escherichia coli )
아우레우스
(Staphylococcus aureus)
Staphylococcus
Aureus
( Staphylococcus aureus )
알비칸스
(Candida albicans)Candida
Albicans
( Candida albicans )
나이거
(Asptergillus niger)Aspergillus
Niger
( Asptergillus niger )
(메틸파라벤)Control
(Methyl paraben)
0.2
0.2
0.2
0.2
0.1
0.1
0.1
0.1
0.1
0.1
상기 표 1에서 보는 바와 같이, 기린초류의 슈도모나스 에어루지노사(Pseudomonas aerugionsa)에 대한 최소생육저해농도는 0.075~0.4%로 나타났으며, 에스케리키아 콜리(Escherichia coli)에 대한 최소생육저해농도는 0.2~1.6%로 나타났고, 스타필로코커스 아우레우스(Staphylococcus aureus)에 대한 최소생육저해농도는 0.025~0.1%로 나타났다. 진균류인 캔디다 알비칸스(Candida albicans)와 아스퍼질러스 나이거(Asptergillus niger)에 대한 최소생육저해농도는 0.1~0.8%인 것으로 나타났다.As shown in Table 1, Pseudomonas Pseudomonas Pseudomonas aerugionsa ) showed a minimum growth inhibition concentration of 0.075 ~ 0.4%, and a minimum growth inhibition concentration of Escherichia coli was 0.2 ~ 1.6%, and Staphylococcus aureus ), Minimum growth inhibition concentration was 0.025 ~ 0.1%. The minimum growth concentrations for fungi Candida albicans and Aspergillus niger were 0.1-0.8 %.
특히, 실시예 4는 세균과 진균에 대해서 메틸파라벤과 동등하거나 우수한 항균력을 나타내었다.
In particular, Example 4 showed the same or superior antimicrobial activity to methyl paraben against bacteria and fungi.
실시예 7 내지 9및 비교예 1 내지 2: 화장료 조성물 제조Examples 7 to 9 and Comparative Examples 1 to 2: Preparation of Cosmetic Composition
다음 처방에 따라 영양크림 제조방법에 의해서 영양크림을 제조하였다.
Nutritional cream was prepared by the nutritional cream manufacturing method according to the following prescription.
폴리솔베이트 60 10 중량%(w/w) Polysorbate 60 10 wt% (w / w)
솔비탄세스퀴올레이트 0.5 중량%(w/w)0.5 wt% sorbitan sesquioleate (w / w)
PEG 경화피마자유 2.0 중량%(w/w)PEG Cured Castor Oil 2.0% by weight (w / w)
유동파라핀 20 중량%(w/w)20% by weight of liquid paraffin (w / w)
스쿠알란 10 중량%(w/w)10% by weight of squalane (w / w)
카프릴릭/카프릭트리글리세라이드 10 중량%(w/w)10% by weight of caprylic / capric triglycerides (w / w)
글리세린 25 중량%(w/w) Glycerin 25 wt% (w / w)
부틸렌글리콜 3.0 중량%(w/w) Butylene glycol 3.0 wt% (w / w)
프로필렌글리콜 3.0 중량%(w/w)Propylene glycol 3.0 wt% (w / w)
트리에탄올아민 0.2 중량%(w/w) 0.2 wt% (w / w) triethanolamine
색소 적량Pigment amount
향료 적량Spices
방부제 또는 유효성분 [표 2]에 중량%(w/w)로 표시Preservative or active ingredient expressed in weight% (w / w) in [Table 2]
정제수를 가하여 100 중량%(w/w)로 조정Adjusted to 100% by weight (w / w) by adding purified water
(섬기린초 에틸아세테이트 분획물)Example 4
(Seomirincho ethyl acetate fraction)
실험 2: Challenge test, 방부력 평가Experiment 2: Challenge test, antiseptic test
상기 실시예 4의 시료를 함유한 화장품 제형 내의 방부력을 알아보기 위해, 영양크림을 제조하여 방부활성 확인 시험을 수행하였다. 방부제 유효성 테스트는 소비자에게 피부 자극을 일으키지 않고 제품을 안전하게 보존하는데 필요로 하는 방부제의 최소농도를 찾기 위한 이유로 제품의 안전성과 소비자 수용에 있어 중요하다. 화장품의 방부력을 측정하는 방법으로 USP와 CTFA의 방법을 많이 사용한다. 본 시험 방법은 미국화장품공업협회(Cosmetic, Toiletry, and Fragrance Association, 이하 CTFA)의 미생물 가이드라인의 시험법을 사용하였다.In order to determine the antiseptic power in the cosmetic formulation containing the sample of Example 4, a nutrition cream was prepared to perform an antiseptic activity test. Preservative effectiveness testing is important for product safety and consumer acceptance as it seeks to find the minimum concentration of preservatives needed to safely preserve the product without causing skin irritation to the consumer. USP and CTFA method are widely used to measure the antiseptic power of cosmetics. This test method used the microbial guidelines of the Cosmetic, Toiletry, and Fragrance Association (CTFA).
Challenge test에 사용한 미생물 균주는 스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa) 및 에스케리치아 콜리(Escherichia coli), 캔디다 알비칸스(Candida albicans) 및 아스퍼질러스 나이거(Aspergillus niger)이고, CTFA의 가이드라인(CTFA Microbiology Guideline) 시험법을 사용하였다. The microbial strain used in the challenge test was Staphylococcus aureus ), Pseudomonas aeruginosa ) and Escherichia coli), Candida albicans (Candida albicans ) and Aspergillus niger , and CTFA Microbiology Guideline assay was used.
항균력 평가와 동일한 방법으로 균을 전 배양하여 세균은 1X106 cfu/g가 되도록 접종하고, 진균은 1X105cfu/g이 되도록 접종한 후, 추출물을 농도별로 첨가한 후, 1~28일까지 시간 경과 관찰을 하였다. 방부력 유효성으로 세균은 접종 7일 이내 99.9% 이상 균 수가 감소해야 하며, 시험기간 동안 증식이 없어야 하고, 미생물 접종 7일 이내 효모와 곰팡이는 적어도 90% 이상 미생물 수가 감소해야 하며, 시험기간 중 지속적으로 미생물 수가 감소해야 한다. Inoculation such that the bacteria in the same manner as in antimicrobial activity evaluation preincubated with bacteria 1X10 6 cfu / g, and the fungus was inoculated so that the 1X10 5 cfu / g, followed by the addition of extract at different concentrations, time to 1 to 28 Progress was observed. As a result of antiseptic effect, bacteria should have reduced 99.9% of bacteria within 7 days of inoculation, no proliferation during the test period, and yeasts and molds should have reduced microorganisms of at least 90% within 7 days of inoculation. The microbial count should be reduced.
다음 표 3과 도 1, 2는 실시예 7 내지 9 및 비교예 1 내지 2에서 제조한 제품에서 시간 경과에 따른 균 수 변화를 나타낸 것이다.
Table 3 and Figures 1 and 2 show the change in the number of bacteria over time in the products prepared in Examples 7 to 9 and Comparative Examples 1 and 2.
균종
Fungus
시료
sample
세균
Germ
진균
Fungus
표 3과 도 1, 2에서 보는 바와 같이, As shown in Table 3 and FIGS. 1 and 2,
실시예 4를 0.1% 이상 함유하는 화장료는 기존 화학 방부제를 사용한 화장료와 거의 동등 수준의 우수한 방부력을 발휘하는 것을 알 수 있다. 이는, 7일차에 세균이 99.9% 감소했고, 진균은 90% 이상 감소했으며, 항균력을 한 달 동안 유지하였다. 따라서 실시예 4는 기존 화학 방부제를 대체할 수 있는 수준이다.
It can be seen that the cosmetic containing 0.1% or more of Example 4 exhibits an excellent antiseptic power at about the same level as the cosmetic using a conventional chemical preservative. This resulted in a 99.9% reduction in bacteria, a 90% reduction in fungi, and antibacterial activity for one month. Therefore, Example 4 is a level that can replace the existing chemical preservatives.
실험 3: 항산화 활성Experiment 3: Antioxidant Activity
기린초류 메탄올 추출물 및 이의 분획물의 항산화 활성은 DPPH (1,1-diphenyl-2-picryl hydrazyl) 음이온 소거능 및 ABTS [2,2-azobis(3-ethylbenzothiazoline-6-sulfonate)] 양이온 소거능 측정으로 평가하였다. 먼저 DPPH 음이온 소거능 측정의 경우, 다양한 농도로 희석한 시료 20 μL에 99.5% 에탄올에 용해시킨 2×10-4M DPPH용액 380 μL를 넣고 혼합하여 37℃에서 30분 동안 반응시킨 후, 516 nm에서 microplate reader (Asys Hitech, Expert96, Asys Co., Austria)를 사용하여 흡광도를 측정하였다. 대조구로는 vitamin C (Sigma Co., USA)를 사용하였다. DPPH free radical 소거능은 시료첨가구와 비첨가구의 백분율로 표시하였으며, IC50는 50% 소거능을 나타내는 농도로 계산하였고 최종 결과는 3회 측정값의 평균으로 나타내었다. ABTS 양이온 소거능 측정의 경우, 7 mM ABTS (Sigma Co., USA) 5 ml와 140 mM potassium persulfate 88 ml를 섞은 후 상온에서 16시간 빛을 차단하여 ABTS 양이온을 형성시켰으며, 이후 이 용액을 414 nm에서 흡광도 값이 1.5가 되도록 에탄올로 희석하였다. 조제된 희석용액 190 ml와 시료 10 ml를 혼합한 후 상온에서 6분간 반응시킨 후 734 nm에서 흡광도를 측정하고 다음의 식에 의해 ABTS 소거능을 결정하였다. Antioxidant activity of giraffe herb methanol extract and fractions thereof was evaluated by DPPH (1,1-diphenyl-2-picryl hydrazyl) anion scavenging activity and ABTS [2,2-azobis (3-ethylbenzothiazoline-6-sulfonate)] cation scavenging activity . First, in the case of DPPH anion scavenging activity, 380 μL of 2 × 10 -4 M DPPH solution dissolved in 99.5% ethanol was mixed in 20 μL of diluted samples at various concentrations, and reacted at 37 ° C. for 30 minutes, and then at 516 nm. Absorbance was measured using a microplate reader (Asys Hitech, Expert96, Asys Co., Austria). Vitamin C (Sigma Co., USA) was used as a control. DPPH free radical scavenging activity was expressed as the percentage of sample addition and non-additional groups. IC 50 was calculated as the concentration showing 50% scavenging activity and the final result was expressed as the average of three measurements. For the determination of ABTS cation scavenging activity, 5 ml of 7 mM ABTS (Sigma Co., USA) and 88 ml of 140 mM potassium persulfate were mixed and light was blocked for 16 hours at room temperature to form ABTS cations. The solution was then 414 nm. Was diluted with ethanol so that the absorbance value was 1.5. 190 ml of the prepared dilution solution and 10 ml of the sample were mixed, and then reacted at room temperature for 6 minutes, and then absorbance was measured at 734 nm, and ABTS scavenging ability was determined by the following equation.
ASA (%) =(C-S)/Cx100, ASA (%) = (C-S) / Cx100,
C: 용매 대조구 DMSO 첨가시의 흡광도, C: absorbance upon addition of solvent control DMSO,
S : 시료 첨가시의 흡광도. S: absorbance at the time of sample addition.
대조구로는 vitamin C (Sigma Co., USA)를 사용하였으며, IC50는 50% 소거능을 나타내는 농도로 계산하였고 최종 결과는 3회 측정값의 평균으로 나타내었다.
Vitamin C (Sigma Co., USA) was used as a control, and IC 50 was calculated as the concentration showing 50% scavenging activity and the final result was expressed as the average of three measurements.
상기 표 4에 나타난 바와 같이 실시예 3의 DPPH 소거능(IC50)은 111.35㎍/ml이고, ABTS 소거능(IC50)은 39.20㎍/ml이다. 실시예 4의 DPPH 소거능(IC50)과 ABTS 소거능(IC50)은 각각 21.48㎍/ml, 6.96㎍/ml이다. 실시예 3에 비해 실시예 4가 더 우수한 DPPH 및 ABTS 소거능을 갖는 것을 알 수 있으며, 실시예 4는 대조구인 vitamin C와 유사한 DPPH 및 ABTS 소거능을 갖는 것을 알 수 있다.
As shown in Table 4, the DPPH scavenging ability (IC 50 ) of Example 3 was 111.35 μg / ml, and the ABTS scavenging ability (IC 50 ) was 39.20 μg / ml. DPPH scavenging ability (IC 50 ) and ABTS scavenging ability (IC 50 ) of Example 4 were 21.48 μg / ml and 6.96 μg / ml, respectively. It can be seen that Example 4 has better DPPH and ABTS scavenging ability than Example 3, and Example 4 has similar DPPH and ABTS scavenging ability to vitamin C as a control.
실험 4: Nitrite 소거능Experiment 4: Nitrite Scavenging Capacity
Nitrite 소거능 측정의 경우, 아질산염 용액(1 mM)에 시료용액을 가하고, 여기에 0.1 N HCl을 가해 pH 1.2로 조정한 후, 37℃에서 1시간 반응시킨 후 Griess reagent (Sigma Co., USA)를 가하고 혼합하였다. 이후 15분간 실온에서 방치 후 520 nm에서 흡광도를 측정하여 잔존 nitrite 양을 측정하였다. In the case of Nitrite scavenging ability, a sample solution was added to nitrite solution (1 mM), 0.1 N HCl was added thereto, adjusted to pH 1.2, and then reacted at 37 ° C for 1 hour, followed by Griess reagent (Sigma Co., USA). Added and mixed. After remaining at room temperature for 15 minutes, the absorbance was measured at 520 nm to determine the amount of residual nitrite.
NSA(%)는 다음의 식에 의해 계산하였다.NSA (%) was calculated by the following formula.
NSA (%) =[1-(A-C)/B]x100, NSA (%) = [1- (A-C) / B] x100,
A: 1 mM nitrite 용액에 시료를 첨가하여 1시간 반응시킨 후의 흡광도, A: absorbance after adding the sample to 1 mM nitrite solution and reacting for 1 hour,
B: 1 mM nitrite 용액의 흡광도, C: 시료의 흡광도. B: absorbance of 1 mM nitrite solution, C: absorbance of sample.
대조구로는 vitamin C (Sigma Co., USA)를 사용하였으며, 용매 대조구로는 DMSO를 사용하였다. 각각의 활성 평가는 각각 3회 이상 반복한 실험의 평균값으로 표시하였으며, 소거능 평가에서 50%의 소거능이 나타나는 시료 농도를 IC50로 나타내었다.
Vitamin C (Sigma Co., USA) was used as a control and DMSO was used as a solvent control. Each activity evaluation was expressed as the average value of the experiment repeated three or more times, and the concentration of the sample showing 50% of the scavenging ability in the scavenging activity test was expressed as IC 50 .
상기 표 5에 실시예 3과 실시예 4의 Nitrite 소거능을 나타내었다. 실시예 3의 Nitirte 소거능(IC50)은 31.95㎍/ml이고, 실시예 4의 Nitirte 소거능(IC50)은 10.49㎍/ml이다. 실시예 3에 비해 실시예 4가 더 우수한 Nitite 소거능을 갖는 것을 알 수 있으며, 실시예 4는 대조구인 vitamin C와 유사한 Nitrite 소거능을 갖는 것을 알 수 있다.Table 5 shows Nitrite scavenging ability of Example 3 and Example 4. The Nitirte scavenging ability (IC 50 ) of Example 3 is 31.95 μg / ml, and the Nitirte scavenging ability (IC 50 ) of Example 4 is 10.49 μg / ml. It can be seen that Example 4 has a better Nitite scavenging ability than Example 3, and Example 4 has a Nitrite scavenging ability similar to vitamin C as a control.
Claims (9)
Antibacterial and antioxidant composition comprising giraffe herb extract as an active ingredient.
상기 기린초류 추출물은 스타필로코커스 아우레우스(Staphylococcus aureus), 슈도모나스 에어루지노사(Pseudomonas aeruginosa), 에스케리치아 콜리(Escherichia coli), 캔디다 알비칸스(Candida albicans), 아스퍼질러스 나이거(Aspergillus niger)에 항균력이 있는 것을 특징으로 하는 항균 및 항산화 조성물.
The method of claim 1,
The giraffe herb extract is Staphylococcus aureus , Pseudomonas Pseudomonas aeruginosa ), Escherichia coli), Candida albicans (Candida albicans ), Aspergillus niger ) antimicrobial and antioxidant composition, characterized in that it has an antimicrobial activity.
상기 기린초류 추출물은 분쇄된 기린초류에 용매를 혼합하여 상온에서 1~2일간 정치하여 제조하거나, 분쇄된 기린초류 추출물에 용매를 혼합하여 상온에서 1~2일간 정치하여 제조된 기린초류 추출물에 에틸아세테이트를 더 가한 후 에틸아세테이트층을 수거하여 제조하는 것을 특징으로 하는 항균 및 항산화 조성물.
The method of claim 1,
The giraffe herb extract is prepared by mixing the solvent in the ground giraffe herb and left to stand at room temperature for 1 to 2 days, or by mixing the solvent with the ground giraffe herb extract and leaving the mixture to stand at room temperature for 1 to 2 days to ethyl. After adding more acetate, the ethyl acetate layer is collected and produced.
상기 기린초류 추출물은 물, C1~C4 알코올, 아세톤, 핵산, 에틸아세테이트, 이산화탄소 중 하나 또는 둘 이상을 혼합한 것을 사용한 용매추출 또는 분획에 의해 제조하는 것을 특징으로 하는 항균 및 항산화 조성물.
The method of claim 1,
The giraffe herb extract is an antimicrobial and antioxidant composition, characterized in that prepared by solvent extraction or fractionation using a mixture of water, C1 ~ C4 alcohol, acetone, nucleic acid, ethyl acetate, carbon dioxide.
상기 조성물은 식품첨가물 형태인 것을 특징으로 하는 항균 및 항산화 조성물.
The method according to any one of claims 1 to 4,
The composition is an antimicrobial and antioxidant composition, characterized in that the food additive form.
상기 조성물은 화장품 조성물 형태인 것을 특징으로 하는 항균 및 항산화 조성물.
The method according to any one of claims 1 to 4,
The composition is an antimicrobial and antioxidant composition, characterized in that in the form of a cosmetic composition.
상기 조성물은 건강기능성 식품 형태인 것을 특징으로 하는 항균 및 항산화 조성물.
The method according to any one of claims 1 to 4,
The composition is an antimicrobial and antioxidant composition, characterized in that the health functional food form.
상기 조성물은 사료첨가제 형태인 것을 특징으로 하는 항균 및 항산화 조성물.
The method according to any one of claims 1 to 4,
The composition is an antimicrobial and antioxidant composition, characterized in that the feed additive form.
상기 조성물은 치료용 약제 조성물 형태인 것을 특징으로 하는 항균 및 항산화 조성물.The method according to any one of claims 1 to 4,
The composition is an antimicrobial and antioxidant composition, characterized in that the therapeutic pharmaceutical composition form.
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KR20150107376A (en) | 2014-03-14 | 2015-09-23 | 주식회사 다인소재 | Antibacterials composition comprising Sedum extract composition and food additives and preparation method thereof |
KR20200120255A (en) * | 2019-04-12 | 2020-10-21 | 주식회사 다인소재 | Anti-MRSA composition comprising element from Sedum takesimense Nakai and beta-lactam antibiotics as active ingredient |
KR102199106B1 (en) * | 2020-07-08 | 2021-01-06 | 주식회사 유렌코리아 | Cosmetic composition for moisturizing and wrinkle improvement containing pericarp |
CN117625308A (en) * | 2023-11-03 | 2024-03-01 | 滁州学院 | Chuzhou chrysanthemum stem and leaf essential oil and application thereof |
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KR20150107376A (en) | 2014-03-14 | 2015-09-23 | 주식회사 다인소재 | Antibacterials composition comprising Sedum extract composition and food additives and preparation method thereof |
KR20200120255A (en) * | 2019-04-12 | 2020-10-21 | 주식회사 다인소재 | Anti-MRSA composition comprising element from Sedum takesimense Nakai and beta-lactam antibiotics as active ingredient |
KR102199106B1 (en) * | 2020-07-08 | 2021-01-06 | 주식회사 유렌코리아 | Cosmetic composition for moisturizing and wrinkle improvement containing pericarp |
CN117625308A (en) * | 2023-11-03 | 2024-03-01 | 滁州学院 | Chuzhou chrysanthemum stem and leaf essential oil and application thereof |
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