KR20120023480A - (a novel lactobacillus plantarum, composition with antibacterial and antioxidant activity comprising the same and use of thereof - Google Patents

(a novel lactobacillus plantarum, composition with antibacterial and antioxidant activity comprising the same and use of thereof Download PDF

Info

Publication number
KR20120023480A
KR20120023480A KR1020100106771A KR20100106771A KR20120023480A KR 20120023480 A KR20120023480 A KR 20120023480A KR 1020100106771 A KR1020100106771 A KR 1020100106771A KR 20100106771 A KR20100106771 A KR 20100106771A KR 20120023480 A KR20120023480 A KR 20120023480A
Authority
KR
South Korea
Prior art keywords
onion
strain
plantarum
lactobacillus plantarum
composition
Prior art date
Application number
KR1020100106771A
Other languages
Korean (ko)
Other versions
KR101197203B1 (en
Inventor
김수기
장우경
조상범
김동운
Original Assignee
건국대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 건국대학교 산학협력단 filed Critical 건국대학교 산학협력단
Publication of KR20120023480A publication Critical patent/KR20120023480A/en
Application granted granted Critical
Publication of KR101197203B1 publication Critical patent/KR101197203B1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/25—Lactobacillus plantarum

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: A novel Lactobacillus plantarum strain and antibacterial and antioxidative probiotics are provided to be used in foods or feed additives. CONSTITUTION: A Lactobacillus plantarum KACC91579P is selected from onions. The strain has an antibacterial or antioxidative activity. A probiotic composition contains the strain. A feed additive composition contains the strain. A medium composition for culturing the strain contains 40-60% of onion juice or onion extract, 0.5-3%(w/v) of sugar, and yeast extract. A method for culturing the strain comprises a step of fermenting in a medium containing 40-60% of onion juice or onion extract.

Description

신규한 락토바실러스 플랜타럼 균주, 그 균주를 포함하는 항균 및 항산화 활성을 가지는 조성물 및 그 용도{(A novel Lactobacillus plantarum, Composition with antibacterial and antioxidant activity comprising the same and use of thereof}A novel Lactobacillus plantarum strain, a composition having antibacterial and antioxidant activity comprising the strain and its use {(A novel Lactobacillus plantarum, Composition with antibacterial and antioxidant activity comprising the same and use of

본 발명은 항균 및 항산화 활성을 가지는 생균제 및 그 용도에 관한 것으로 더욱 상세하게는 발효 양파로부터 선별된 신규한 락토바실러스 플랜타럼(Lactobacillus plantarum) SK1990 및 그 균주를 포함한 생균제 조성물, 그 균주의 배양 배지 조성 및 그 배양방법에 관한 것이다.The present invention relates to a probiotic having antibacterial and antioxidant activity and its use, and more particularly, to a novel Lactobacillus plantarum selected from fermented onions. plantarum ) SK1990 and a probiotic composition comprising the strain, the culture medium composition of the strain and the method of culturing the same.

가축은 사육하는 동안 스트레스를 받으면 장내 병원성 미생물이 증가하고 유익균수가 감소한다. 유해균이 생성한 독소는 장점막의 활성을 둔화시켜 사료의 효율을 떨어뜨리고 독소에 의한 장점막 괴사로 질병발생의 원인이 된다. 상기와 같은 원인을 제거하고 가축의 생산성을 높이기 위하여 생균제가 이용되고 있다.When livestock are stressed during breeding, they increase intestinal pathogenic microorganisms and reduce the number of beneficial bacteria. The toxin produced by harmful bacteria slows down the activity of the intestinal mucosa, reducing the feed efficiency and causing the disease by necrosis caused by the toxin. Probiotics have been used to eliminate such causes and to increase livestock productivity.

생균제는 사람이나 동물이 섭취했을 때 위장관에 머물러 생존하면서 특정 병리적 상태를 예방하거나 치료하여 유익한 효과를 주는 미생물을 말한다. 이 생균제는 장내 유익한 미생물의 균수와 활성을 증가시켜 장내 균총을 정상으로 회복시키기는 역할을 한다.Probiotics are microorganisms that, when ingested by humans or animals, live in the gastrointestinal tract and survive, preventing or treating certain pathological conditions and giving a beneficial effect. The probiotics increase the number and activity of beneficial microorganisms in the intestine and restore the intestinal flora to normal.

오늘날과 같이 가축 사육시 항생제와 각종 합성 약제의 사용이 범람하고 있는 시대에 가축을 건강하게 사육하기 위하여는 생균제와 같은 사료첨가제가 필요하며, 이에 대한 연구가 진행되고 있다.In today's era, when the use of antibiotics and various synthetic drugs is inundated in livestock raising, feed additives such as probiotics are needed to keep livestock healthy.

특허 제381726호(명칭: 양식용 사료첨가제로서의 감귤 발효액)에는 감귤 발효액을 이용하여 양식어류용 사료첨가제에 대하여 개시되어 있다.Patent 381726 (name: citrus fermentation broth as aquaculture feed additive) discloses a feed additive for aquaculture fish using a citrus fermentation broth.

특허공개 제1999-0046622호(명칭: 축산용 무화과사료 및 이의 제조방법)에는 무화과 잎과 열매를 이용하여 축산용 사료를 제조하는 방법이 개시되어 있다.Patent Publication No. 1999-0046622 (name: animal feed for animal husbandry and its manufacturing method) discloses a method for producing animal feed using fig leaves and fruits.

특허 제336411호(명칭: 고온 혐기성 유산발효 혼합사료 제조 방법)에는 40~70℃의 고온에서 혐기성 유산발효하여 사료를 제조하는 방법이 개시되어 있다.Patent No. 336411 (name: method for producing high temperature anaerobic lactic acid fermentation mixed feed) discloses a method for producing a feed by anaerobic lactic acid fermentation at a high temperature of 40 ~ 70 ℃.

상기한 종래기술로는 사료 내에 함유된 병원성 미생물의 성장을 억제하는 데에 한계가 있었고 또한 가축의 육질을 개선하는 데에도 한계가 있었다.The above-described prior art has a limit in suppressing the growth of pathogenic microorganisms contained in the feed and also in improving the meat quality of the livestock.

본 발명은 상기의 문제점을 해결하고 상기의 필요성에 의하여 안출된 것으로서 본 발명의 목적은 가축의 생산성 향상에 도움이 되는 생균제를 제공하는 것이다.The present invention solves the above problems and the object of the present invention is to provide a probiotic that helps to improve the productivity of livestock.

본 발명의 다른 목적은 항균 및 항산화 활성을 가지는 생균제를 제공하는 것이다.Another object of the present invention is to provide a probiotic having antibacterial and antioxidant activity.

본 발명의 또 다른 목적은 상기 균주를 배양하는 배지 및 배양 조건을 제공하는 것이다.Another object of the present invention is to provide a medium and culture conditions for culturing the strain.

상기의 목적을 달성하기 위하여 본 발명은 양파로부터 선발된 락토바실러스 플랜타럼(Lactobacillus plantarum) SK1990 KACC91579P를 제공한다.In order to achieve the above object, the present invention is Lactobacillus plantarum ( Lactobacillus) selected from onions plantarum ) SK1990 KACC91579P.

본 발명의 상기 균주는 대한민국 수원시 소재 국립농업과학원 농업유전자원센터에 2010년 8월 23일에 수탁번호 KACC91579P로 기탁하였다.The strain of the present invention was deposited with the accession number KACC91579P on August 23, 2010 to the National Institute of Agricultural Science, National Institute of Agricultural Science, Suwon, Korea.

본 발명의 일 구체예에 있어서, 상기 균주는 항균 또는 항산화 활성을 가지는 것이 바람직하나 이에 한정되지 아니한다.In one embodiment of the present invention, the strain is preferably one having an antimicrobial or antioxidant activity, but is not limited thereto.

또한 본 발명은 상기 본 발명의 균주를 포함하는 생균제 조성물을 제공한다.The present invention also provides a probiotic composition comprising the strain of the present invention.

또한 본 발명은 상기 본 발명의 균주를 포함하는 사료 첨가제 조성물을 제공한다.The present invention also provides a feed additive composition comprising the strain of the present invention.

또한 본 발명은 락토바실러스 플랜타럼 배양 배지에 있어서, 양파즙 또는 양파 추출물을 포함하는 락토바실러스 플랜타럼 배양 배지 조성물을 제공한다.The present invention also provides a Lactobacillus plantarum culture medium composition comprising onion juice or onion extract in the Lactobacillus plantarum culture medium.

본 발명의 일 구현예에 있어서, 배양 배지 조성물은 설탕 및 효모 추출물을 더욱 포함하는 것이 바람직하나 이에 한정되지 아니한다.In one embodiment of the present invention, it is preferable that the culture medium composition further comprises a sugar and yeast extract, but is not limited thereto.

본 발명의 다른 구현예에 있어서, 상기 양파즙 또는 양파 추출물은 전체 조성물에서 40%?60%, 설탕은 0.5?3%(w/v) 포함되는 것이 바람직하나 이에 한정되지 아니한다.In another embodiment of the present invention, the onion juice or onion extract 40% ~ 60% in the total composition, the sugar is preferably included 0.5 ~ 3% (w / v), but is not limited thereto.

또한 본 발명은 락토바실러스 플랜타럼 배양 방법에 있어서, 배지에 양파즙 또는 양파 추출물을 전체 배양에서 40%?60%(w/v)포함하여 발효하는 단계를 포함하는 락토바실러스 플랜타럼 배양 방법을 제공한다.In another aspect, the present invention provides a method for culturing Lactobacillus plantarum, comprising the step of fermenting 40% ~ 60% (w / v) of the onion juice or onion extract in the culture medium in the medium do.

본 발명의 제제는 항산화 및 항균 갖는 수의학적 제제 및 식품/사료 첨가제로서 사용될 수 있다. The formulations of the invention can be used as veterinary formulations with antioxidant and antibacterial and food / feed additives.

본 발명에 사용된 용어 "식품 또는 사료 첨가제" 는 사람에게 사용하기 위한 식품 첨가제 또는 영양학적 첨가제, 또는 기타 사육 동물 뿐만 아니라 가축 및 가금류에 사용되는 사료첨가제 또는 영양학적 첨가제를 의미하는 것이다.The term "food or feed additive" as used herein means a food additive or nutritional additive for use in humans, or a feed additive or nutritional additive used in livestock and poultry as well as other farm animals.

이하 본 발명을 설명한다.Hereinafter, the present invention will be described.

먼저 본 발명은 축산용 유용 생균제 균주의 생물학적 활성 평가 및 균주 선발하였다.First, the present invention was to evaluate the biological activity and strain selection of useful probiotic strains for livestock.

본 발명의 일 구체예에 있어서, 본 발명은 가축의 생산성 향상에 도움이 되는 생균제 개발을 위하여 유용 균주를 스크리닝하였으며,In one embodiment of the present invention, the present invention screened useful strains for the development of probiotics to help improve the productivity of livestock,

발효된 양파를 대상으로 유용 세균을 스크리닝하였고 생리활성 (소화효소활성, 항균활성)을 선발 기준으로 하였다(표 1).Useful bacteria were screened for fermented onions and their physiological activities (digestive enzyme activity, antimicrobial activity) were selected as the selection criteria (Table 1).

또한 본 발명은 가축 병원성 세균인 Clostridium perfringens , Escherichia coli, Burkholderia sp., Mannheimia heamolytica를 대상으로 후보 균주들의 항균활성 능력을 평가하였으며(표 1), 후보 균주 중에서 선발된 11종의 균주에 대하여 배양성과 배양액의 항산화활성을 평가하였고(표 2), 선발균주들의 16S rRNA 유전자 염기서열을 분석하였고, 그 결과를 통하여 동정결과를 분석하였다(표 3).In addition, the present invention Clostridium is a livestock pathogenic bacteria perfringens , Escherichia coli, Burkholderia sp., Mannheimia The antimicrobial activity of candidate strains was evaluated in heamolytica (Table 1), and the culture and antioxidant activity of 11 strains selected from the candidate strains were evaluated (Table 2). The sequence was analyzed and the results were identified (Table 3).

이를 통하여 본 발명은 최종적으로 SK1990을 유용 생균제 균주로 선발하였다.Through this, the present invention finally selected SK1990 as a useful probiotic strain.

또한 본 발명은 경제적 배지 선발을 위한 양파즙의 첨가 농도를 결정하였다.The present invention also determined the concentration of onion juice for economic medium selection.

본 발명의 일 구체예에 있어서, 일련의 실험과정을 통하여 항균활성, 항산화활성 그리고 배양적 특성을 고려하여 최종적으로 선발된 SK1990 균주 (Lactobacillus plantarum)의 경제적 배지 선발을 위하여 양파를 주요 탄소원으로 결정할 경우, 사용이 가능한 양파즙의 첨가농도를 결정하기 위하여 본 발명을 수행하였다. In one embodiment of the present invention, SK1990 strain ( Lactobacillus) finally selected in consideration of antimicrobial activity, antioxidant activity and culture characteristics through a series of experiments In order to determine the onion as the main carbon source for economic medium selection of plantarum ), the present invention was performed to determine the concentration of onion juice that can be used.

이에 대한 배양조건은 Working volume: 50ml/200ml, Incubation temperature: 30, Incubation time: 24 hours, Agitation: 150 rpm이었으며, 배양에 사용된 기본배지인 MRS배지와 양파즙 그리고 물의 비율(MRS:양파즙:물)은 100:0:0 (Run1), 20:0:80 (Run2), 20:20:60 (Run3), 20:40:40 (Run4), 20:60:20 (Run5)로 하였다.The culture conditions for this were Working volume: 50ml / 200ml, Incubation temperature: 30, Incubation time: 24 hours, Agitation: 150 rpm, and the ratio of MRS medium, onion juice, and water (MRS: onion juice: Water) were 100: 0: 0 (Run1), 20: 0: 80 (Run2), 20:20:60 (Run3), 20:40:40 (Run4), and 20:60:20 (Run5).

분석항목은 항균활성으로 agar well diffusion assay (African Health Science 2009, 9:S42-46)를 이용하였으며, 테스트 병원균으로는 Salmonella typhimurium ATCC 35150, Listeria monocytogens KACC 10550 및 Streptococcus suis ATCC 700794를 사용하였으며,The analysis items were agar well diffusion assay (African Health Science 2009, 9: S42-46) as antimicrobial activity, Salmonella typhimurium ATCC 35150, Listeria monocytogens KACC 10550 and Streptococcus suis ATCC 700794 were used as test pathogens.

항산화활성은 DPPH 방법(Natural Product Research, 2010, 24:804-812)을 이용하였고, 총균수는 viable cell counts를, Total quercetin equivalent flavonoid content (Food Microbiology, 2010, 27:586-591)를 통하여 수행하였다.Antioxidant activity was performed by DPPH method (Natural Product Research, 2010, 24: 804-812), total cell count was performed by viable cell counts, Total quercetin equivalent flavonoid content (Food Microbiology, 2010, 27: 586-591) It was.

또한 본 발명은 L. plantarum SK1990을 이용한 양파발효의 최적화를 제공한다.In addition, the present invention is L. plantarum It provides optimization of onion fermentation using SK1990.

본 발명의 일 구체예에 있어서, 최적 배지조성의 탐색을 위한 탄소원으로 sucrose와 onion juice를 사용하였고, 질소원으로는 yeast extract를 사용하였다.In one embodiment of the present invention, sucrose and onion juice were used as the carbon source for the search of the optimum medium composition, and yeast extract was used as the nitrogen source.

배지성분들간의 효과를 분석하고 최적배지를 도출하기 위하여 3가지 변수에 대한 full factorial design을 설계하였고, 24시간동안 배양한 후에 viable cell count와 DPPH를 이용한 항산화활성을 조사하였다(표 5).In order to analyze the effect between the media components and to derive the optimal medium, we designed a full factorial design for three variables, and investigated the antioxidant activity using viable cell count and DPPH after incubation for 24 hours (Table 5).

1) 배지성분들이 선발균주 L. plantarum SK1990의 성장에 미치는 효과1) Effect of Medium Components on the Growth of Selected Strain L. plantarum SK1990

결과로 얻어진 viable cell count들의 분산분석을 수행한 결과 선발된 균주의 성장에 있어서는 yeast extract가 유의성 있는 주효과로 나타내었다(표 6).As a result of variance analysis of the resulting viable cell counts, yeast extract showed a significant main effect in the growth of selected strains (Table 6).

또한 양파를 포함하는 배지에서 선발균주 L. plantarum SK1990의 성장에 미치는 요인들의 주효과를 분석한 결과, 모든 요인들이 positive effect를 나타내었으며, sucrose의 경우에서는 곡률효과가 나타내었다 (도 5).In addition, as a result of analyzing the main effects of the factors on the growth of the selection strain L. plantarum SK1990 in the medium containing onion, all the factors showed a positive effect, in the case of sucrose showed a curvature effect (Fig. 5).

또 양파를 포함하는 배지에서 선발균주 L. plantarum SK1990의 성장에 있어 각 요인들의 상호작용을 분석한 결과 양파와 sucrose간에 상호효과가 있는 경향이 관찰되었으며, 양파를 600 g/L 까지 사용할 경우 sucrose는 15 g/L를 사용하는 것이 효율적인 것으로 나타내었다 (도 6).In addition, as a result of analyzing the interactions between the factors in the growth of the selection strain L. plantarum SK1990 in the medium containing onion, there was a tendency of interaction between onion and sucrose. Using 15 g / L has been shown to be efficient (FIG. 6).

2) 배지성분들이 선발균주 L. plantarum SK1990의 항산화활성에 미치는 효과2) Effect of Medium Components on Antioxidant Activity of Selected Strain L. plantarum SK1990

결과로 얻어진 항산화활성들의 분산분석을 수행한 결과, 선발된 균주의 항산화활성에 있어서는 onion juice가 유의성 있는 주효과로 나타내었다(표 7).As a result of variance analysis of the obtained antioxidant activities, onion juice was shown as a significant main effect in the antioxidant activity of the selected strains (Table 7).

또 양파를 포함하는 배지에서 선발균주 L. plantarum SK1990의 성장에 미치는 요인들의 주효과를 분석한 결과, sucrose를 제외한 onion juice와 yeast extract에서 positive effect를 나타났으나, yeast extract는 미약한 효과를 나타내었다 (도 7).In addition, as a result of analyzing the main effects of the growth factors of L. plantarum SK1990 on the medium containing onions, the onion juice and yeast extract except sucrose showed a positive effect, but the yeast extract showed a weak effect. (Figure 7).

또한 양파를 포함하는 배지에서 선발균주 L. plantarum SK1990의 항산화활성에 있어 각 요인들의 상호작용을 분석한 결과 sucrose와 yeast extract간에 상호효과가 있는 경향이 관찰되었으며, sucrose를 15 g/L를 적용하였을 경우에 yeast extract 소요량을 최소화할 수 있었다 (도 8).In addition, as a result of analyzing the interaction of each factor on the antioxidant activity of the selection strain L. plantarum SK1990 in the medium containing onion, there was a tendency of interaction between sucrose and yeast extract, and sucrose was applied to 15 g / L. In this case, yeast extract requirement could be minimized (FIG. 8).

3) 양파를 포함하는 최적배지조성의 도출 및 발효성상 분석3) Derivation of optimum medium composition containing onion and analysis of fermentation properties

상기와 같은 일련의 결과를 통하여 표 8과 같은 최적배지조성을 도출하였다.The optimum medium composition as shown in Table 8 was derived through the above series of results.

5L jar fermenter를 이용하여 최적조건의 배지조성에서 선발균주 L. plantarum SK1990의 발효성상을 분석한 결과 도 9와 같은 결과를 얻을 수 있었다. As a result of analyzing the fermentation properties of the L. plantarum SK1990 selection strain in the medium composition of the optimum conditions using a 5L jar fermenter was obtained as shown in FIG.

본 발명을 통하여 알 수 있는 바와 같이 본 발명에서 선별된 신규한 균주 L. plantarum SK1990의 경우, 항균 및 항산화 활성을 가지고 있어서 이 균주를 식품 또는 사료 첨가제용 생균제로 사용할 수 있다.As can be seen through the present invention, the novel strain L. plantarum SK1990 selected in the present invention has antibacterial and antioxidant activity, and thus, the strain can be used as a probiotic for food or feed additives.

도 1은 배지에 여러 농도의 양파 즙에 대한 L. plantarum SK1990의 성장 프로파일을 나타낸 그래프이다.
도 2는 배지에 여러 농도의 양파 즙에 대한 L. plantarum SK1990의 항산화 활성 프로파일을 나타낸 그래프이다.
도 3은 Salmonella typhimurium (A), Listeria monocytogens (B) and Streptococcus suis (C)에 대한 여러 수준의 양파 즙으로 배양된 L. plantarum SK1990의 항균 활성을 나타낸 그래프이다.
도 4는 여러 수준의 양파 즙을 가진 L. plantarum SK1990의 배양에서 퀘르세틴 균등물 전체 플라보노이드 프로파일을 나타낸 그래프이다.
도 5는 L. plantarum SK1990의 세포 성장에 대한 변수들의 주된 효과를 나타낸 그래프이다.
도 6은 Lactobacillus plantarum SK1990의 세포 성장에 대한 변수들의 상호작용을 나타낸 그래프이다.
도 7은 Lactobacillus plantarum SK1990의 항산화 활성에 대한 변수들의 주된 효과를 나타낸 그래프이다.
도 8은 Lactobacillus plantarum SK1990의 항산화 활성에 대한 변수들의 상호작용을 나타낸 그래프이다.
도 9는 양파즙을 함유한 적정화된 배지에서 Lactobacillus plantarum SK1990의 발효 프로파일을 나타낸 그래프이다. pH는 10N NaOH를 사용하여서 5.0을 최소범위로 하여 6.0으로 조절되었다. 1 is a graph showing the growth profile of L. plantarum SK1990 for different concentrations of onion juice in the medium.
Figure 2 is a graph showing the antioxidant activity profile of L. plantarum SK1990 for different concentrations of onion juice in the medium.
3 Salmonella typhimurium (A), Listeria A graph showing the antimicrobial activity of L. plantarum SK1990 cultured with different levels of onion juice against monocytogens (B) and Streptococcus suis (C).
4 is a graph showing the quercetin equivalent total flavonoid profile in the culture of L. plantarum SK1990 with different levels of onion juice.
5 is a graph showing the main effects of variables on cell growth of L. plantarum SK1990.
6 Lactobacillus plantarum It is a graph showing the interaction of variables on the cell growth of SK1990.
7 is Lactobacillus plantarum It is a graph showing the main effects of the variables on the antioxidant activity of SK1990.
8 is Lactobacillus plantarum This is a graph showing the interaction of variables on the antioxidant activity of SK1990.
Figure 9 Lactobacillus in a titrated medium containing onion juice Graph showing the fermentation profile of plantarum SK1990. The pH was adjusted to 6.0 using 10N NaOH with a minimum of 5.0.

이하 비한정적인 실시예를 통하여 본 발명을 더욱 상세하게 설명한다. 단 하기 실시예는 본 발명을 예시하기 위한 목적으로 기재된 것으로서 본 발명의 범위는 하기 실시예에 의하여 제한되는 것으로 해석되지 아니한다.
The present invention will now be described in more detail by way of non-limiting examples. However, the following examples are described for the purpose of illustrating the present invention and the scope of the present invention is not to be construed as limited by the following examples.

실시예Example 1: 유용 균주의 스크리닝 방법 1: Screening Method of Useful Strains

유용균주의 스크리능을 위하여 경북울진군에서 재배된 국내산 양파를 분쇄기 (Philips HR2860, Netherlands)로 곱게 갈은 양파즙에 토양을 인위적으로 섞고 상온에서 자연적으로 발효될 수 있도록 하였다. 이때에 세균의 원활한 증식을 위하여 MRS, LB, PD (Difco, USA)를 1:2 (양파즙:배지)의 비율로 첨가하였다. 자연발효의 진행이 완료된 발효양파액을 첨가한 배지와 동일한 조성의 평판배지에 도말하여 서로 형태가 다른 집락을 분리하였다. For the screening of useful strains, domestic onions grown in Gyeongbuk Uljin-gun were finely ground with a grinder (Philips HR2860, Netherlands), and the soil was artificially mixed and allowed to ferment naturally at room temperature. At this time, MRS, LB, PD (Difco, USA) was added in a ratio of 1: 2 (onion juice: medium) for the smooth growth of bacteria. The colonies of the same composition as the medium to which the fermentation onions, which had undergone the fermentation of natural fermentation, were plated to separate the colonies.

분리된 균주들의 생리활성을 알아보기 위하여 소화효소활성과 균활성 정도를 평가하였다. 소화효소의 종류로는 전분분해효소, 단백질 분해효소, 섬유소 분해효소 그리고 지방분해효소 등을 평가하였으며, 각각 soluble starch, skim milk, carboxymethyl cellulose 그리고 tributirin을 평판배지에 1.0%씩 혼합하여 제조하였고 선발균주를 도말하여 배양하여 분비된 소화효소에 의해 형성된 투명대의 크기로 활성정도를 평가하였다. 전분분해효소의 투명대는 KI (0.2%)와 iodine (0.2%)를 혼합한 용액으로 염색하여 관찰하였고, 섬유소분해효소는 congo red (0.2%)로 염색하여 관찰하였다. 단백질과 지방분해효소는 배양 후 자연적으로 발생된 투명대를 관찰하였다. Digestive enzyme activity and fungal activity were evaluated to determine the physiological activity of the isolates. Digestive enzyme, starch, proteolytic enzyme, fibrinolytic enzyme, and lipolytic enzyme were evaluated. Digestive enzymes were prepared by mixing 1.0% of soluble starch, skim milk, carboxymethyl cellulose and tributirin in plate media. Activity was assessed by the size of the zona pellucida formed by secreting digestive enzymes by smearing. The zona pellucida of starch enzyme was observed by staining with a mixture of KI (0.2%) and iodine (0.2%), and fibrinase was observed by staining with congo red (0.2%). Proteins and lipolytic enzymes observed the zona pellucida naturally occurring after incubation.

항균활성은 가축병원성 세균들을 대상으로 중층배지를 이용한 agar well diffusion assay 방법을 사용하였다. Antimicrobial activity was determined by agar well diffusion assay using medium media against livestock pathogenic bacteria.

Figure pat00001
Figure pat00001

Figure pat00002
Figure pat00002

표 1은 분리된 균주의 소화 효소 및 항균 활성을 나타낸 표이다.Table 1 is a table showing the digestive enzymes and antimicrobial activity of the isolated strain.

실시예Example 2:선발된 균주에 대한 배양성과 배양액의 항산화활성을 평가 2: Evaluate the culture and antioxidant activity of the selected strains

상기 표로부터 선정된 11종의 균주에 대한 배양조건은 Working volume: 50ml/200ml, Incubation temperature: 30, Incubation time: 24 hours, Agitation: 150 rpm이었으며, The culture conditions for 11 strains selected from the table were Working volume: 50ml / 200ml, Incubation temperature: 30, Incubation time: 24 hours, Agitation: 150 rpm,

분석항목은 항균활성으로 agar well diffusion assay(African Health Science 2009, 9:S42-46)를 이용하였으며, 테스트 병원균으로는 Salmonella typhimurium ATCC 35150, Listeria monocytogens KACC 10550 및 Streptococcus suis ATCC 700794를 사용하였으며,Analytical items were agar well diffusion assay (African Health Science 2009, 9: S42-46) for antimicrobial activity. Salmonella typhimurium ATCC 35150, Listeria as test pathogens monocytogens KACC 10550 and Streptococcus suis ATCC 700794 were used.

항산화활성은 DPPH 방법(Natural Product Research, 2010, 24:804-812)을 이용하였고, 총균수는 viable cell counts를, Total quercetin equivalent flavonoid content (Food Microbiology, 2010, 27:586-591)를 통하여 수행하였다.Antioxidant activity was performed by DPPH method (Natural Product Research, 2010, 24: 804-812), total cell count was performed by viable cell counts, Total quercetin equivalent flavonoid content (Food Microbiology, 2010, 27: 586-591) It was.

그 결과는 하기 표 2와 같다.The results are shown in Table 2 below.

Figure pat00003
Figure pat00003

표 2는 선정된 균주들의 자유 래디컬 소거 활성 및 그들의 성장 특성을 나타낸 표이다.Table 2 is a table showing the free radical scavenging activity and their growth characteristics of the selected strains.

실시예Example 3:  3: 선발균주들의Selection of strains 16S  16S rRNArRNA 유전자 염기서열을 분석 Gene sequence analysis

상기 선발균주들의 16S rRNA 유전자 염기서열을 분석하였고, 그 결과를 통하여 동정결과를 분석하였다(표 3).The 16S rRNA gene sequences of the selected strains were analyzed, and the identification results were analyzed through the results (Table 3).

Strain No.Strain No. Identity, %Identity,% SK1968SK1968 Delftia tsuruhatensisDelftia tsuruhatensis 9999 SK1969SK1969 EnterobacterEnterobacter ludwigiiludwigii 100100 SK1974SK1974 Stenotrophomonas maltophilia Stenotrophomonas maltophilia 100100 SK1976SK1976 PseudomonasPseudomonas putidaputida 9999 SK1977SK1977 LactococcusLactococcus lactislactis 100100 SK1978SK1978 LactococcusLactococcus lactislactis 100100 SK1979SK1979 PseudomonasPseudomonas plecoglossicidaplecoglossicida 100100 SK1982SK1982 PseudomonasPseudomonas plecoglossicidaplecoglossicida 100100 SK1983SK1983 BacillusBacillus simplexsimplex 9999 SK1984SK1984 LeuconostocLeuconostoc citreumcitreum 9999 SK1990SK1990 LactobacillusLactobacillus plantarumplantarum 9999

표 3은 선발 균주들의 16S rRNA 유전자 서열과 NCBI의 BLAST에 의한 호모로지 서치 결과이다.Table 3 shows the results of homology search by 16S rRNA gene sequence of the selected strains and BLAST of NCBI.

실시예Example 4: 본 발명의 균주 배양을 위한 배지 조성에서 적정 양파 즙 농도 결정 4: Determination of the appropriate onion juice concentration in the medium composition for culturing the strain of the present invention

상기에서 본 발명의 균주로 선별된 L. plantarum SK1990의 배양을 위한 배지 조성에서 적정 양파 즙 농도의 선택을 위한 실험 디자인을 하기 표 4에 기재된 것과 같이 디자인하였다.Experimental design for the selection of the appropriate onion juice concentration in the medium composition for the culture of L. plantarum SK1990 selected as the strain of the present invention was designed as described in Table 4 below.

조성Furtherance 처리구1) Treatment Zone 1) Run 1Run 1 Run 2Run 2 Run 3Run 3 Run 4Run 4 Run 5Run 5 MRS broth,%MRS broth,% 100100 2020 2020 2020 2020 양파즙,%Onion Juice,% 00 00 2020 4040 8080 증류수,%Distilled water,% 00 8080 6060 4040 00 전체,%all,% 100100 100100 100100 100100 100100 1)처리구: 배지(MRS):양파즙:물의 비율 Run1, 100:0:0; Run2, 20:0:80; Run3, 20:20:60; Run4, 20:40:40; Run5, 20:60:20. 1) treatment: medium (MRS): onion juice: water ratio Run1, 100: 0: 0; Run 2, 20: 0: 80; Run 3, 20:20: 60; Run 4, 20: 40: 40; Run 5, 20:60:20.

표 4는 L. plantarum SK1990의 배양을 위한 배지 조성에서 적정 양파 즙 농도의 선택을 위한 실험 디자인을 나타낸 표이다.
Table 4 is a table showing the experimental design for the selection of the appropriate onion juice concentration in the medium composition for the culture of L. plantarum SK1990.

실시예Example 5: 배지 성분들 간의 효과 분석 및 최적 배지 도출 5: Analyzing effects between media components and deriving optimal media

L. plantarum SK1990의 배양을 위한 배지성분들간의 효과를 분석하고 최적배지를 도출하기 위하여 3가지 변수에 대한 full factorial design을 설계하였고, 24시간동안 배양한 후에 viable cell count와 DPPH를 이용한 항산화활성을 조사하였다(표 5). In order to analyze the effect between the media components for cultivation of L. plantarum SK1990 and to derive the optimal medium, we designed a full factorial design for three variables and investigated the antioxidant activity using viable cell count and DPPH after incubation for 24 hours. (Table 5).

또한 결과로 얻어진 viable cell count들의 분산분석을 수행한 결과 선발된 균주의 성장에 있어서는 yeast extract가 유의성 있는 주효과로 나타내었다(표 6).In addition, as a result of performing variance analysis of the resultant viable cell counts, yeast extract showed a significant main effect in the growth of selected strains (Table 6).

또한 결과로 얻어진 항산화활성들의 분산분석을 수행한 결과, 선발된 균주의 항산화활성에 있어서는 onion juice가 유의성 있는 주효과로 나타내었다 (표 7). In addition, as a result of variance analysis of the resulting antioxidant activity, onion juice was shown as a significant main effect in the antioxidant activity of the selected strains (Table 7).

RunsRuns 양파즙Onion Juice SucroseSucrose 효모 추출물Yeast extract 세포 수율,
Log10(cfu/mL)Cell yield,
Log 10 (cfu / mL) 자유 래디컬 소거 활성, %Free radical scavenging activity,% g/Lg / L 1One 400400 55 1One 8.828.82 38.138.1 22 400400 55 55 8.958.95 40.740.7 33 400400 1515 1One 8.788.78 39.239.2 44 400400 1515 55 9.159.15 38.838.8 55 400400 3030 1One 9.059.05 36.636.6 66 400400 3030 55 8.998.99 39.739.7 77 600600 55 1One 8.828.82 47.047.0 88 600600 55 55 8.978.97 47.247.2 99 600600 1515 1One 9.099.09 47.547.5 1010 600600 1515 55 8.948.94 42.742.7 1111 600600 3030 1One 8.738.73 44.944.9 1212 600600 3030 55 9.159.15 47.947.9 1313 400400 55 1One 8.908.90 37.737.7 1414 400400 55 55 8.888.88 40.340.3 1515 400400 1515 1One 8.648.64 38.238.2 1616 400400 1515 55 8.988.98 36.636.6 1717 400400 3030 1One 8.908.90 35.335.3 1818 400400 3030 55 8.888.88 38.938.9 1919 600600 55 1One 8.788.78 44.244.2 2020 600600 55 55 8.828.82 44.844.8 2121 600600 1515 1One 8.948.94 50.550.5 2222 600600 1515 55 9.059.05 45.745.7 2323 600600 3030 1One 9.009.00 44.844.8 2424 600600 3030 55 9.029.02 49.549.5

표 5는 Lactobacillus plantarum SK1990의 자유 래디컬기 소거 활성 및 성장에 대한 인자의 효과를 평가하기 위한 전체 요소 디자인의 형태를 나타낸 표이다.Table 5 shows Lactobacillus plantarum This is a table showing the form of the overall element design for evaluating the effect of the factors on the free radical scavenging activity and growth of SK1990.

인자factor DFDF SSSS MSMS FF PP 양파onion 1One 0.006000.00600 0.006000.00600 0.590.59 0.4580.458 SucroseSucrose 22 0.040920.04092 0.020460.02046 2.012.01 0.1770.177 효모 추출물Yeast extract 1One 0.075260.07526 0.075260.07526 7.387.38 0.0190.019 양파ⅹSucroseSucrose 22 0.026470.02647 0.013230.01323 1.301.30 0.3090.309 양파ⅹ효모 추출물Onion ⅹ Yeast Extract 1One 0.000790.00079 0.000790.00079 0.080.08 0.7860.786 Sucroseⅹ효모 추출물Sucrose Yeast Extract 22 0.010280.01028 0.005140.00514 0.500.50 0.6160.616 양파ⅹSucroseⅹ효모 추출물Onion ⅹ Sucrose ⅹ Yeast Extract 22 0.106790.10679 0.053390.05339 5.245.24 0.0230.023 ErrorError 1212 0.122300.12230 0.010190.01019 전체all 2323 0.388800.38880

표 6은 Lactobacillus plantarum SK1990의 성장에 대한 효소들의 효과에 대한 분산분석표이다.Table 6 shows Lactobacillus plantarum An analysis of variance of the effects of enzymes on the growth of SK1990.

인자factor DFDF SSSS SSSS MSMS FF PP 양파onion 1One 0.03903470.0390347 0.03903470.0390347 0.03903470.0390347 224.30224.30 0.0000.000 SucroseSucrose 22 0.00003370.0000337 0.00003370.0000337 0.00001690.0000169 0.100.10 0.9080.908 효모 추출물Yeast extract 1One 0.00033340.0003334 0.00033340.0003334 0.00033340.0003334 1.921.92 0.1920.192 양파ⅹSucroseSucrose 22 0.00068650.0006865 0.00068650.0006865 0.00034330.0003433 1.971.97 0.1820.182 양파ⅹ효모 추출물Onion ⅹ Yeast Extract 1One 0.00049290.0004929 0.00049290.0004929 0.00049290.0004929 2.832.83 0.1180.118 Sucroseⅹ효모 추출물Sucrose Yeast Extract 22 0.00439080.0043908 0.00439080.0043908 0.00219540.0021954 12.6212.62 0.0010.001 양파ⅹSucroseⅹ효모 추출물 Onion ⅹ Sucrose ⅹ Yeast Extract 22 0.00043760.0004376 0.00043760.0004376 0.00021880.0002188 1.261.26 0.3190.319 Error Error 1212 0.00208840.0020884 0.00208840.0020884 0.00017400.0001740 전체all 2323 0.04749800.0474980

표 7은 Lactobacillus plantarum SK1990의 자유 래디컬 소거 활성에 대한 분산분석표이다.Table 7 shows Lactobacillus plantarum An analysis of variance of free radical scavenging activity of SK1990.

실시예Example 6: 양파를 포함하는  6: containing onion 최적배지조성의Optimal medium composition 도출 및  Derivation and 발효성상Fermentation property 분석 analysis

상기와 같은 일련의 결과를 통하여 표 8과 같은 최적배지조성을 도출하였다.The optimum medium composition as shown in Table 8 was derived through the above series of results.

5L jar fermenter를 이용하여 최적조건의 배지조성에서 선발균주 L. plantarum SK1990의 발효성상을 분석한 결과 도 9와 같은 결과를 얻을 수 있었다. As a result of analyzing the fermentation properties of the L. plantarum SK1990 selection strain in the medium composition of the optimum conditions using a 5L jar fermenter was obtained as shown in FIG.

조성Furtherance Content, g/LContent, g / L 양파 즙Onion Juice 600600 SucroseSucrose 1515 효모 추출물Yeast extract 55 K2HPO4 K 2 HPO 4 0.20.2 MgCl2 MgCl 2 0.020.02 CH3COONaCH 3 COONa 1One

표 8은 L. plantarum SK1990로 양파 발효의 항산화 활성의 개량을 위한 배지 조성물의 최적 조성을 나타낸 표이다. Table 8 is a table showing the optimum composition of the medium composition for improving the antioxidant activity of onion fermentation with L. plantarum SK1990.

농업생명공학연구원Institute of Agricultural Biotechnology KACC91579KACC91579 2010082320100823

Claims (8)

양파로부터 선발된 락토바실러스 플랜타럼(Lactobacillus plantarum) KACC91579P. Lactobacillus Screened from Onion plantarum ) KACC91579P. 제 1항에 있어서, 상기 균주는 항균 또는 항산화 활성을 가지는 특징으로 하는 락토바실러스 플랜타럼(Lactobacillus plantarum) KACC91579P. According to claim 1, The strain Lactobacillus ( Lactobacillus) characterized in that it has an antimicrobial or antioxidant activity plantarum ) KACC91579P. 제 1항 또는 제2항의 균주를 포함하는 생균제 조성물.Probiotic composition comprising the strain of claim 1 or 2. 제 1항 또는 제2항의 균주를 포함하는 사료 첨가제 조성물.Feed additive composition comprising the strain of claim 1. 락토바실러스 플랜타럼 배양 배지에 있어서, 양파즙 또는 양파 추출물을 포함하는 락토바실러스 플랜타럼 배양 배지 조성물.Lactobacillus plantarum culture medium, Lactobacillus plantarum culture medium composition comprising onion juice or onion extract. 제 5항에 있어서, 배양 배지 조성물은 설탕 및 효모 추출물을 더욱 포함하는 락토바실러스 플랜타럼 배양 배지 조성물.6. The Lactobacillus plantarum culture medium composition according to claim 5, wherein the culture medium composition further comprises sugar and yeast extract. 제 5항 또는 제 6항에 있어서, 상기 양파즙 또는 양파 추출물은 전체 조성물에서 40%?60%, 설탕은 0.5?3%(w/v)포함되는 것을 특징으로 하는 락토바실러스 플랜타럼 배양 배지 조성물.The Lactobacillus plantarum culture medium composition according to claim 5 or 6, wherein the onion juice or onion extract comprises 40% to 60% of the total composition and 0.5 to 3% (w / v) of sugar. . 락토바실러스 플랜타럼 배양 방법에 있어서, 배지에 양파즙 또는 양파 추출물을 전체 배양에서 40%?60%(w/v)포함하여 발효하는 단계를 포함하는 락토바실러스 플랜타럼 배양 방법.In the Lactobacillus plantarum culturing method, Lactobacillus plantarum cultivation method comprising the step of fermenting onion medium or onion extract in the culture medium 40% ~ 60% (w / v) in the total culture.
KR1020100106771A 2010-08-16 2010-10-29 A novel Lactobacillus plantarum, Composition with antibacterial and antioxidant activity comprising the same and use of thereof KR101197203B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100078931 2010-08-16
KR1020100078931 2010-08-16

Publications (2)

Publication Number Publication Date
KR20120023480A true KR20120023480A (en) 2012-03-13
KR101197203B1 KR101197203B1 (en) 2012-11-02

Family

ID=46130960

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020100106771A KR101197203B1 (en) 2010-08-16 2010-10-29 A novel Lactobacillus plantarum, Composition with antibacterial and antioxidant activity comprising the same and use of thereof

Country Status (1)

Country Link
KR (1) KR101197203B1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180035480A (en) 2016-09-29 2018-04-06 (주)아모레퍼시픽 Novel chromobacterium aquaticum dolsongi ht-2 having antioxidant activity and use thereof
KR20180035481A (en) 2016-09-29 2018-04-06 (주)아모레퍼시픽 Novel hafnia alvei dolsongi ht-3 having antioxidant activity and use thereof
KR102254751B1 (en) * 2020-01-09 2021-05-24 한국식품연구원 New Lactobacillus plantarum WiKim83 and use thereof
KR102254752B1 (en) * 2020-01-09 2021-05-24 한국식품연구원 New Lactobacillus plantarum WiKim87 and use thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102063772B1 (en) 2019-07-23 2020-01-08 주식회사 메디오젠 lactobacillus having blood glucose lowering effect and antioxidant effect

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180035480A (en) 2016-09-29 2018-04-06 (주)아모레퍼시픽 Novel chromobacterium aquaticum dolsongi ht-2 having antioxidant activity and use thereof
KR20180035481A (en) 2016-09-29 2018-04-06 (주)아모레퍼시픽 Novel hafnia alvei dolsongi ht-3 having antioxidant activity and use thereof
KR102254751B1 (en) * 2020-01-09 2021-05-24 한국식품연구원 New Lactobacillus plantarum WiKim83 and use thereof
KR102254752B1 (en) * 2020-01-09 2021-05-24 한국식품연구원 New Lactobacillus plantarum WiKim87 and use thereof

Also Published As

Publication number Publication date
KR101197203B1 (en) 2012-11-02

Similar Documents

Publication Publication Date Title
CN103555640B (en) Bacillus subtilis and application thereof to livestock breeding
KR101197203B1 (en) A novel Lactobacillus plantarum, Composition with antibacterial and antioxidant activity comprising the same and use of thereof
KR101591879B1 (en) Synbiotic feed additive affecting gut microbiota and growth performance of livestock
Nithya et al. Characterization of bacteriocin producing lactic acid bacteria and its application as a food preservative
Świeca et al. Lactobacillus plantarum 299V improves the microbiological quality of legume sprouts and effectively survives in these carriers during cold storage and in vitro digestion
CN1819771A (en) A probiotic composition comprising at least two lactic acid bacterial strains which are able to colonise the gastrointestinal tracts in combination with having intestinal survival property, intestinal
KR20090030661A (en) A feed additive for raising fishes, a feed composition, and a breeding method thereof
Dumitru et al. Preliminary characterisation of Bacillus subtilis strain use as a dietary probiotic bio-additive in weaning piglet
Akbulut et al. Identification and potential biotechnological characterization of lactic acid bacteria isolated from white cheese samples
JP2016526900A (en) Isolated microbial strain Lactobacillus plantarum TAK59 NCIMB42150 and uses thereof
KR101317549B1 (en) Method for producing fermented corn gluten
Uugantsetseg et al. Antioxidant activity of probiotic lactic acid bacteria isolated from Mongolian airag
KR101451810B1 (en) Novel Strains of Lactobacillus plantarum K255 with High Productivity of Gamma-Aminobutyric Acid
Ziadi et al. Screening of lactic starter from Tunisian fermented vegetables and application for the improvement of caper (Capparis spinosa) fermentation through an experimental factorial design
JP5893772B1 (en) Method for producing lactic acid bacteria and fermented product
KR101155158B1 (en) Novel bacillus subtilis 5-b strain and composition for forage additive comprising the same
KR20190047756A (en) A composition comprising lactobacillus probiotics and a functional feed additive comprising the same
KR20070071911A (en) Novel lactobacillus sakei and use thereof
KR101034467B1 (en) Microorganism cultivating medium containing enzymatic hydrolysate of wheat bran and konjak
Adisa et al. Microbiological and probiotic assessment of yeast isolated from wholegrain millet sourdoughs
KR100398478B1 (en) Fermented Feeds Containing Ume and Their Preparation Method
KR20140022652A (en) New leuconostoc citreum and food including the same
El-Sayed et al. Characterization, encapsulation and evaluation of the newly isolated Enterococcus faecium as a probiotic for ruminants
JP6831111B2 (en) Meat quality improvement method
KR20160045278A (en) A fermented feed additive composition using microorganism and a prepration method thereof

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20151125

Year of fee payment: 4

FPAY Annual fee payment

Payment date: 20161019

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee