KR20100018813A - Cosmetic composition cpmprising mixture extracts of orostachys japonicus, selaginella tamariscina, lonicera japonica thunberg and smilax china for anti-inflammation and antioxidation - Google Patents
Cosmetic composition cpmprising mixture extracts of orostachys japonicus, selaginella tamariscina, lonicera japonica thunberg and smilax china for anti-inflammation and antioxidation Download PDFInfo
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Abstract
Description
본 발명은 항염 및 항산화용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for anti-inflammatory and antioxidant.
염증은 세균과 같은 외부 이물질의 침입과 상처로부터 인체를 보호하는 생리적인 반응이다. 염증 반응은 우리 몸에서의 항상성 유지를 위한 자극에 대한 생체의 방어 반응으로 lipopolysaccride(LPS리포폴리사카라이드)와 같은 외부 자극원과 arachidonic acid(아라키돈산)와 같은 내부 자극원들을 매개로 한다. 인체는 염증현상을 통하여 다양한 종류의 단백질 분해효소와 사이토카인(cytokine)등의 세포 생성물을 분비함으로써 치료 및 방어를 할 수 있게 한다. Inflammation is a physiological response that protects the body from invasion and invasion of foreign objects such as bacteria. The inflammatory response is a biological defense response to stimuli to maintain homeostasis in our body, which is mediated by external stimuli such as lipopolysaccride (LPS lipopolysaccharide) and internal stimulants such as arachidonic acid. The human body secretes various kinds of proteolytic enzymes and cytokines (cytokine) through inflammation to enable treatment and defense.
그러나 이러한 염증작용이 과도하게 일어나면 단백질 분해 효소들에 의해 주변 세포 및 결합조직이 손상을 입고 결합 조직의 손상은 피부의 탄력을 감소시켜 주름의 원인이 될 뿐 아니라 나아가 세포의 재생 및 증식에도 나쁜 영향을 미치게 되어 빠른 피부노화를 초래하게 된다. 따라서 적절한 조건하에서는 염증은 초기 상태가 지난 후에는 정상기능을 되찾게 되지만 염증과정이 지속적 또는 만성적으로 계속되면 심각한 조직의 손상을 가져오게 된다.However, excessive inflammatory action causes damage to surrounding cells and connective tissues by proteolytic enzymes, and damage to connective tissues reduces skin elasticity, which causes wrinkles and also adversely affects cell regeneration and proliferation. This leads to rapid skin aging. Thus, under appropriate conditions, inflammation may resume normal function after the initial phase, but if the inflammatory process continues or continues chronically, serious tissue damage may occur.
한편, 활성산소 또는 자유라디칼은 진피의 결합조직인 콜라켄, 엘라스틴, 히알루론산 등을 파괴하여 피부의 일정 부위에 침하현상(주름)을 일으키며 세포막의 지질 부분을 산화시켜 세포의 파괴 현상을 일으켜 피부염, 여드름, 피부암 등의 질병을 유발한다. 또한 활성산소는 멜라닌 형성과정 중 자발적인 산화반응에 관여하여 기미, 주근깨 등의 원인 및 주름 생성의 원인이 되기도 한다.Active oxygen or free radicals destroy collagen, elastin, and hyaluronic acid, which are the connective tissues of the dermis, causing subsidence (wrinkle) in certain areas of the skin, and oxidizing the lipid part of the cell membrane to cause cell destruction. It causes diseases such as acne and skin cancer. In addition, active oxygen is involved in the spontaneous oxidation reaction during melanin formation process, causing spots, freckles, and wrinkles.
따라서 최근 활성산소 또는 자유라디칼을 조절할 수 있는 물질인 항산화제에 대한 연구가 활발히 진행되어 효소계열의 예방적 항산화제인 SOD(superoxide dismutase), 카탈라아제(catalase), 글루타치온 퍼옥시다아제(glutathione peroxidase) 등과 천연항산화제인 토코페롤(tocopherol), 아스코르빈산(ascorbic acid), 카로테노이드(carotenoid), 글루타치온(glutathione) 및 합성항산화제인 BHA, BHT 등 많은 항산화제가 개발되어 있다. 그러나 이러한 약물들은 고가일 뿐만 아니라 배합시 안전성 및 안정성이 좋지 못하여 실질적인 효과를 얻기가 어려운 문제점이 있다. Therefore, recently, active research on antioxidants, which can regulate free radicals or free radicals, has been actively conducted, and natural antioxidants such as SOD (superoxide dismutase), catalase, glutathione peroxidase, etc. Many antioxidants have been developed, including tocopherol, ascorbic acid, carotenoids, glutathione, and synthetic antioxidants BHA and BHT. However, these drugs are not only expensive but also have poor safety and stability in combination, making it difficult to obtain a practical effect.
따라서 이러한 자유라디칼 및 염증에 의한 피부 트러블의 개선을 위해서는 항염 및 항산화 효과가 우수하며, 부작용이 없고 비용이 저렴하며 안정성이 우수하여 사용이 용이한 화장료의 개발이 요구된다. Therefore, in order to improve the skin troubles caused by free radicals and inflammation, it is necessary to develop cosmetics that are excellent in anti-inflammatory and antioxidant effects, have no side effects, are inexpensive, have excellent stability, and are easy to use.
한편 바위솔(Orostachys japonicus.)은 돌나물과 식물로 산지의 양지바른 바위틈이나 전통가옥의 기와와 돌각담에 붙어서 서식하나, 지금은 찾아보기 어려운 실정이다. 요즘은 멸종 단계에 이르러 인위적으로 배양, 증식 시켜 약용으로 사용하고 있다. 예로부터 학질, 간염, 습진, 이질설사, 치질, 악성종기, 화상 등의 치료에 쓰였으며, 종기나 상처에 짓찧어 붙이면 고름을 빨아내는 효과가 큰 것으로 알려져 있다. 혈류량 촉진, 진통, 면역, 피부염, 모발 성장 촉진 및 피부가 갈라지는 피부염에도 효과가 있는 것으로 알려져 있다. Orostachys japonicus .) is a sedum and plant that lives on sunny rocky crevices in the mountains or on the tiles and stone walls of traditional houses. Nowadays, it has reached extinction stage and is artificially incubated and used for medicinal purposes. Since ancient times, it has been used for the treatment of malaria, hepatitis, eczema, dysentery diarrhea, hemorrhoids, malignant boils, and burns. It is known to be effective in promoting blood flow, analgesic, immunity, dermatitis, promoting hair growth, and cracking dermatitis.
부처손(Selaginella tamariscina) 예로부터 여성들의 자궁출혈이나 생리불순, 생리통에 효험이 크고 치질, 장출혈, 탈항, 피오줌, 자궁냉증으로 불임을 고통 받는 여성 등에도 좋다고 알려져 있다. 또한 만성 간염, 간경화증, 황달, 기침, 신장결석, 정신분열증, 갖가지 암, 기관지염, 폐렴, 편도선염에도 효험이 있으며 노인들이 힘이 없고 몸이 나른할 때 부처손을 달여 먹으면 기운이 난다고 한다. Buddha Selaginella tamariscina ) Since ancient times, women are known to be effective in uterine bleeding, menstrual disorders and menstrual cramps, and are also good for women suffering from infertility due to hemorrhoids, intestinal bleeding, prolapse, pissing, and hypothermia. It is also effective in chronic hepatitis, cirrhosis, jaundice, cough, kidney stones, schizophrenia, various cancers, bronchitis, pneumonia, tonsillitis.
인동덩굴(Lonicera japonica thunberg)은 폐, 위경에 작용하며 열을 내리고 해독하는 효능이 있어 급성 열병으로 인한 발열, 열독으로 인하여 생긴 적리, 큰 종기, 종독, 간질, 급성 전염병, 갈증해소, 옴, 혈액보충, 장염, 세균성 이질, 홍역, 이하선염, 패혈증, 맹장염, 유행성 B형 뇌염, 유행성 뇌척수막염, 담도감염, 급성인후염, 매독에서 오는 가래톳, 골수염, 급성유선염, 유방암, 비인암, 중만기 암환자의 재감염, 인후부종류(咽喉部腫瘤), 농약중독, 폐렴, 젖먹이 어린이 설사, 화농성 질환, 자궁경미란, 눈 급성 염증, 두드러기, 외상감염, 어린이 땀띠, 감기, 나력, 치루, 일사병 치료에 효과가 있다고 알려져 있다. Honeysuckle ( Lonicera japonica thunberg ) acts on the lungs and stomach, and has the effect of lowering and detoxifying heat, causing fever due to acute fever, heat caused by heat poisoning, big boils, poison, epilepsy, acute infectious diseases, thirst quenching, scabies and blood Replenishment, enteritis, bacterial dysentery, measles, mumps, sepsis, appendicitis, pandemic encephalitis B, epidemic meningitis, biliary tract infection, acute sore throat, sputum from syphilis, osteomyelitis, acute mastitis, breast cancer, non-human cancer, re-infection of middle-aged cancer patients , Sore throat poisoning, pesticide poisoning, pneumonia, suckling diarrhea, purulent disease, uterine erosion, eye acute inflammation, urticaria, trauma infection, children's sweating, cold, nausea, dental floss, heat stroke .
청미래덩굴(Smilax china)은 동의치료에서 습을 내보내며 열내림과 피를 맑게 하고 오줌내기, 독풀이 효과가 있다 하여 매독, 창독, 만성 피부병, 수은중독성 피부염에 쓴다. 또한 풍습성 관절염, 콩팥염, 방광염, 소화가 안되고 설사할 때에 15~31g을 물에 달여 먹는다. 민간에서 소화기암(식도암, 위암, 결장암)과 코암, 자궁암에 부처손, 까마중과 같이 써서 효과를 보았다고 한다. Smilax china is used for syphilis, window poisoning, chronic skin disease and mercury poisoning dermatitis because it releases moisture from motion therapy, clears the blood, clears the blood, pee and poisons. In addition, customary arthritis, kidney disease, cystitis, indigestion and diarrhea when eat 15 ~ 31g in water to eat. It is said that it has been used in civilian digestive cancer (esophageal cancer, stomach cancer, colon cancer), nose cancer, and uterine cancer with Buddha hand and kamjung.
이에 본 발명자들은 바위솔, 부처손, 인동덩굴 및 청미래덩굴의 혼합 추출물이 매우 우수한 항염 및 항산화 등의 효능을 나타냄을 발견하고 본 발명을 완성하였다.The present inventors have found that the mixed extract of rock brush, Buddha hand, honeysuckle and blue mule vine showed very good anti-inflammatory and antioxidant effect and completed the present invention.
따라서 본 발명의 목적은 바위솔, 부처손, 인동덩굴 및 청미래덩굴의혼합추출물을 함유하는 항염 및 항산화 효과가 있는 화장료 조성물을 제공하고자 하는 것이다.Therefore, it is an object of the present invention to provide a cosmetic composition having an anti-inflammatory and antioxidant effect, which contains a mixed extract of rock brush, Buddha hand, honeysuckle and blue mule vine.
본 발명은 바위솔, 부처손, 인동덩굴 및 청미래덩굴의 혼합추출물을 함유하는 항염 및 항산화용 화장료 조성물을 제공한다. The present invention provides a cosmetic composition for anti-inflammatory and antioxidant containing a mixed extract of rock brush, Buddha hand, honeysuckle and blue mule vine.
본 발명에서 사용되는 바위솔, 부처손, 인동덩굴 및 청미래덩굴은 국내외 산등에서 직접 채취하거나, 재래시장 또는 한약재를 파는 곳에서 구입하여 사용할 수 있다. 이들은 채취하거나 구입한 후 세척하여 건조시킨 후, 세절한 후에 본 발명의 추출물 제조에 사용될 수 있다.Rock brush, Buddha hand, honeysuckle and cheongmirae vines used in the present invention can be collected directly from domestic and foreign mountains, or can be purchased and used in traditional markets or selling herbal medicines. These may be used for preparation of the extract of the present invention after harvesting or purchasing, washing, drying and then cutting.
본 발명에서 바위솔, 부처손 인동덩굴 및 청미래덩굴의 다양한 중량비로 혼합되어 사용될 수 있다. 예들 들어, 바위솔, 부처손, 인동덩굴 및 청미래덩굴을 1: 0.25~4: 0.25~4: 0.25~4 중량비로 혼합될 수 있으며, 바람직한 혼합 중량비는 1:1:1:1 이다.In the present invention, it can be used by mixing in various weight ratios of rock brush, Buddha hand honeysuckle and blue rice vine. For example, rock brush, Buddha hand, honeysuckle and blue vine can be mixed in a weight ratio of 1: 0.25-4: 0.25-4: 0.25-4, the preferred mixing weight ratio is 1: 1: 1: 1.
본 발명의 화장료 조성물에 포함되는 바위솔, 부처손, 인동덩굴 및 청미래덩 굴의 혼합추출물의 제조방법을 예로 들면 도1에 나타낸 바와 같다. 바위솔, 부처손, 인동덩굴 및 청미래덩굴을 건조 및 파쇄하고, 선택적으로 초임계 전처리하여 멸균하고 농약 및 불순물을 제거한 후, 냉각콘덴서가 달린 추출기에서, 추출용매로 유효성분을 추출하고, 추출물을 회수하고 농축하여 제조한다. 바람직하게는 본 발명의 혼합추출물은 바위솔, 부처손, 인동덩굴 및 청미래덩굴을 초임계 전처리 한 후 추출한 것이 바람직하다. 상기 초임계 전처리 조건은 높은 압력(100~800bar)과 낮은 온도(40~70℃)에서 실시되는 초임계 이산화탄소 전처리 방법이며, 초임계 전처리 된 혼합물은 멸균 및 농약을 제거한 후에 에탄올로 50~100℃에서 3~24시간 동안 추출한 후 농축하여 제조될 수 있다.For example, a method of manufacturing a mixed extract of a rock brush, a Buddha hand, a honeysuckle, and a blue rice vine contained in the cosmetic composition of the present invention is shown in FIG. 1. Drying and crushing rock brush, Buddha hand, honeysuckle, and blue vine, selectively sterilize by supercritical pretreatment, remove pesticides and impurities, extract the active ingredient with the extracting solvent in the extractor with cooling capacitor, recover the extract, Prepared by concentration. Preferably, the mixed extract of the present invention is preferably extracted after the supercritical pretreatment of rock brush, Buddha hand, honeysuckle vine and blue miracle vine. The supercritical pretreatment is a supercritical carbon dioxide pretreatment method carried out at high pressure (100 ~ 800bar) and low temperature (40 ~ 70 ℃), the supercritical pre-treated mixture is 50 ~ 100 ℃ with ethanol after sterilization and removal of pesticides After extracting for 3 to 24 hours can be prepared by concentration.
초임계 전처리 방법은 고압 공정으로 인한 멸균 효과가 있고 낮은 온도 처리이기 때문에 열에 쉽게 반응하는 유효성분이 쉽게 변성되거나 파괴되지 않으며 별도의 유기용매를 전혀 사용하지 않기 때문에 잔류 유기용매가 없고, 불순물이 적어 보다 피부에 안전한 유효성분을 추출할 수 있다는 장점이 있다. 바위솔의 유효 성분인 에둘틴, 부처손의 유효 성분인 이노시톨, 인동덩굴의 유효 성분인 루테오린-7-그함노글리코사이드과 청미래덩굴의 휴효 성분인 스밀라사포닌B은 초임계 이산화탄소에 용해성이 없고 100℃ 이하에서 변성이 없기 때문에 초임계 전처리 과정을 거친 후 에탄올로 추출하여도 유효성분의 변성이 없다. Since the supercritical pretreatment has a sterilization effect due to the high pressure process and the low temperature treatment, the active ingredient that reacts easily to heat is not easily denatured or destroyed, and since there is no separate organic solvent, there is no residual organic solvent and less impurities. It has the advantage of extracting effective ingredients that are safe for the skin. Edultin, the active ingredient in rock brush, Inositol, the active ingredient in Buddha's hands, and Luteolin-7-Ghamnoglycoside, the active ingredient in honeysuckle, and Smilasaponin B, the active ingredient in blue vines, are insoluble in supercritical carbon dioxide and 100 ℃ Since there is no denaturation in the following, even after extraction with ethanol after the supercritical pretreatment, there is no denaturation of the active ingredient.
본 발명의 혼합추출물 제조에 사용되는 용매는 당업자에게 알려진 추출용매이면 어느 것이든 가능하다. 예를 들어, 물, 메탄올, 에탄올, 프로판올, 부탄올과 같은 C1~C4의 알코올, 또는 함수 C1~C4 알코올, 부틸렌글리콜 과 같은 C1~C6의 글리콜, 함수 C1~C6의 글리콜, 에틸아세테이트, 헥산, 벤젠, 디에틸에테르, 디클로로메탄 등이 있으며, 단일 또는 2종 이상의 혼합용매를 혼합하여 사용할 수 있다. 바람직한 천연 식물 추출용매는 에탄올을 포함하는 C1~C4의 알코올, 함수 C1~C4알코올이다.The solvent used in the preparation of the mixed extract of the present invention may be any extraction solvent known to those skilled in the art. For example, C1-C4 alcohols such as water, methanol, ethanol, propanol, butanol, or C1-C6 glycols such as hydrous C1-C4 alcohols, butylene glycol, glycols of hydrous C1-C6, ethyl acetate, hexane , Benzene, diethyl ether, dichloromethane, and the like, and a single or two or more mixed solvents may be mixed and used. Preferred natural plant extractants are C1-C4 alcohols containing ethanol, hydrous C1-C4 alcohols.
본 발명에서 00%알코올은 00(v/v)% 알코올과 나머지 부분의 물을 함유하고 있는 알코올을 의미한다.In the present invention, 00% alcohol means an alcohol containing 00 (v / v)% alcohol and the remainder of the water.
또한 본 발명의 화장료 조성물은 현재 시판되는 화장품, 화장용비누,화장용 세안제의 제형을 가진다. 예를 들어, 본 발명의 화장료 조성물은 화장수(스킨로션), 영양로션, 영양크림, 맛사지 크림, 에센스, 팩 또는 유화형 화운데이션와 같은 화장품 제형일 수 있다. 또한, 본 발명의 화장료 조성물은 유아전용 화장수(스킨로션), 로션, 크림, 자외선차단 로션 또는 자외선차단 크림과 같은 제형일 수 있다. 또한, 본 발명의 화장료 조성물은 유아전용 목욕 세정제, 샴푸 또는 비누와 같은 화장용 세정제의 제형일 수 있다.In addition, the cosmetic composition of the present invention has a formulation of commercially available cosmetics, cosmetic soap, cosmetic face wash. For example, the cosmetic composition of the present invention may be a cosmetic formulation such as a lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, essence, pack or emulsified foundation. In addition, the cosmetic composition of the present invention may be a formulation such as baby lotion (skin lotion), lotion, cream, sunscreen lotion or sunscreen cream. In addition, the cosmetic composition of the present invention may be a formulation of a cosmetic cleaner, such as a baby bath cleaner, shampoo or soap.
이들 각 제형은 그 제형의 제제화에 필요한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.Each of these formulations may contain various bases and additives necessary for the formulation of the formulations, and the types and amounts of these components may be readily selected by those skilled in the art.
본 발명의 화장료 조성물에서, 추출물의 함량의 사용목적 및 사용자의 대상에 따라 다양하게 함유될 수 있다. 바람직한 혼합추출물의 함량은 화장료 조성물 총중량에 대하여 0.1 내지 10.0 중량% 이다.In the cosmetic composition of the present invention, the content of the extract may be contained in various ways depending on the purpose of use and the user's object. The preferred amount of the mixed extract is 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.
본 발명의 화장료 조성물은 상기 혼합추출물 이외에 화장료 조성물에 통상적으로 이용되는 성분들을 포함할 수 있으며, 예를 들면, 안정화제, 용매화제, 비타민, 안료 및 향료와 같은 통상적인 보조제 및 담체를 포함할 수 있다.The cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the mixed extract, and may include, for example, conventional auxiliaries and carriers such as stabilizers, solvating agents, vitamins, pigments and flavors. have.
또한 본 발명에 따른 항염 및 항산화 효과를 가지는 화장료 조성물은 통상적인 사용방법에 따라 사용될 수 있으며, 사용자의 피부 상태 또는 취향에 따라 그 사용 횟수를 달리할 수 있다.In addition, the cosmetic composition having an anti-inflammatory and antioxidant effect according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.
본 발명의 화장료 조성물에서 바위솔, 부처손, 인동덩굴 및 청미래덩굴에 함유된 필수 지방산인 감마리놀렌산 성분은 피부 보습과 염증 현상을 조절하며, 이 감마리놀렌산은 각질층의 세포들을 촘촘하게 하여 피부 속의 유분 및 수분을 보호하여 피부가 건조해지면서 탄력을 잃어버리는 것을 막아 준다.In the cosmetic composition of the present invention, gamma linolenic acid, which is an essential fatty acid contained in rock brush, bud, hand, honeysuckle, and blue rice vine, regulates skin moisturizing and inflammation, and the gamma linolenic acid densely protects cells of the stratum corneum to protect oil and moisture in the skin. This prevents the skin from drying out and loses its elasticity.
또한, 인체에서 지질과산화, 세포내 자유라디칼 생성, 기타 라디칼 반응을 억제함으로써 항염 및 항산화 효과를 나타낸다.It also exhibits anti-inflammatory and antioxidant effects by inhibiting lipid peroxidation, intracellular free radical production, and other radical reactions in the human body.
이하, 실시예, 비교예, 대조예, 제제예, 실험예 등을 본 발명의 구성 및 효과를 상세히 설명하나, 본 발명이 이들로 한정되는 것은 아니다. Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples, Comparative Examples, Control Examples, Formulation Examples, and Experimental Examples, but the present invention is not limited thereto.
<< 실시예Example 1 내지 7>바위솔, 부처손, 1 to 7> rock brush, buddha hand, 인동덩굴Honeysuckle 및 청미래덩굴의 혼합 추출물 제조 And preparation of mixed extract of blue vines
하기 표1의 시료 혼합 비율(바위솔:부처손:인동덩굴:청미래덩굴)로 한 시료를 분쇄하여 만든 분말 100g을 100~800bar, 40~70℃ 조건에서 초임계 전처리 한 후, 95(v/v)% 함수 에탄올 (5(v/v)%의 물을 함유한 에탄올) 1㎏에 넣고 냉각 콘덴서가 달린 추출기에서 60℃로 3시간 끓여서 추출하였다. 이후 400메쉬 여과포로 여과하고, 1주일간 방치하여 침전물을 0.45㎛ 필터로 여과하였다. 그리고 감압 농축기를 이용하여 50℃에서 완전히 농축하여 건조 중량 표 1에 기재된 만큼 얻었다.100 g of powder prepared by pulverizing a sample of the sample mixing ratio (rock brush: Buddha hand: honeysuckle: blue vine) shown in Table 1 was subjected to supercritical pretreatment at 100 to 800 bar and 40 to 70 ° C, and then 95 (v / v). 1 kg of% hydrous ethanol (ethanol containing 5 (v / v)% of water) was added and extracted by boiling at 60 ° C. for 3 hours in an extractor equipped with a cooling condenser. Thereafter, the filtrate was filtered through a 400 mesh filter cloth, and the precipitate was left to stand for one week, and the precipitate was filtered through a 0.45 μm filter. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain the dry weight as shown in Table 1.
<< 실시예Example 8 내지 10> 8 to 10>
바위솔,부처손,인동덩굴 및 청미래덩굴의 조성 비율을 1:1:1:1중량비 즉, 25g:25g:25g:25g으로 하고 하기 표2의 용매를 사용한 것을 제외하고 실시예1과 동일한 방법으로 추출하였다. 그 결과는 하기 표2와 같다. The composition ratio of rock brush, Buddha hand, honeysuckle and blue mule vine is 1: 1: 1: 1 by weight ratio, that is, 25g: 25g: 25g: 25g and extracted in the same manner as in Example 1 except for using the solvent shown in Table 2 below. It was. The results are shown in Table 2 below.
<< 비교예1Comparative Example 1 > 바위솔 추출물의 제조> Preparation of Rock Brush Extract
실시예1에서 사용한 혼합 분말대신에 바위솔을 분쇄하여 만든 분말 100g을 사용한 것을 제외하고 실시예1과 동일한 방법으로 추출하여, 표제의 추출물을 건조 중량 32.33g 얻었다.Extracting was carried out in the same manner as in Example 1, except that 100 g of a powder obtained by pulverizing rock brush was used instead of the mixed powder used in Example 1 to obtain a dry weight of 32.33 g of the title extract.
<< 비교예2Comparative Example 2 > 부처손 추출물의 제조> Preparation of Buddha Hand Extract
실시예 1에서 사용한 혼합 분말대신에 부처손을 분쇄하여 만든 분말 100g을 사용한 것을 제외하고 실시예1과 동일한 방법으로 추출하여, 표제의 추출물을 건조 중량 33.62g을 얻었다.Extracting was carried out in the same manner as in Example 1, except that 100 g of the powder obtained by pulverizing the Buddha instead of the mixed powder used in Example 1 was used to obtain a dry weight of 33.62 g of the title extract.
<< 비교예3Comparative Example 3 > > 인동덩굴Honeysuckle 추출물의 제조 Preparation of Extract
실시예1에서 사용한 혼합 분말대신에 인동덩굴을 분쇄하여 만든 분말 100g을 상용한 것을 제외하고 실시예1과 동일한 방법으로 추출하여, 표제의 추출물을 건조 중량 36.27g을 얻었다.100 g of powder obtained by pulverizing phosphorus vines was used instead of the mixed powder used in Example 1, and extracted in the same manner as in Example 1 to obtain a dry weight of 36.27 g of the title extract.
<< 비교예4Comparative Example 4 > 청미래덩굴 추출물의 제조> Preparation of Blue Marrow Extract
실시예1에서 사용한 혼합 분말 대신에 청미래덩굴을 분쇄하여 만든 분말 100g을 사용한 것을 제외하고 실시예1과 동일한 방법으로 추출하여, 표제의 추출물을 건조 중량 32.87g 얻었다.Extracted in the same manner as in Example 1, except that 100 g of the powder produced by crushing the blue mule vines was used instead of the mixed powder used in Example 1 to obtain a dry weight of 32.87 g of the title extract.
<< 대조예1Comparative Example 1 >> 인도매타신Indomethacin (카운터)의 제조Manufacture of (counter)
상용화된 인도메타신 결정(Indomethacin Crystalline 7378, Sigma Chemical Co.)을 사용하여 95% 에탄올 1ml에 녹여1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, 1000 ㎍/㎖ 농도별로 인도메타신 용액을 제조하였다.Indomethacin Crystalline 7378 (Sigma Chemical Co.) was used to dissolve in 1 ml of 95% ethanol. Was prepared.
<< 대조예2Comparative Example 2 >> 녹차씨Green tea seed (카운터) 추출물의 제조Preparation of (Counter) Extract
녹차씨를 분쇄한 100g을 비교예1의 방법으로 제조하여 건조 중량 36.98g을 얻었다.100 g of green tea seed was pulverized by the method of Comparative Example 1 to obtain a dry weight of 36.98 g.
<< 대조예Control 3 내지 5> 추출용매의 함수 함량 변화에 따른 3 to 5> Changes in Water Content of Extraction Solvents 녹차씨Green tea seed 추출물의 제조 Preparation of Extract
녹차씨를 분쇄한 100g을 표2의 용매를 추출용매로 사용하여 실시예 1과 동일한 추출방법으로 추출하여 그 결과를 하기 표3에 기재하였다.100 g of green tea seed was pulverized and extracted using the same extraction method as Example 1 using the solvent of Table 2 as an extraction solvent, and the results are shown in Table 3 below.
< < 대조예Control 6> 6> 리포폴리사카라이드Lipopolysaccharide (( LPSLPS ) 정제) refine
리포폴리사카라이드(구입처: 전남대학교 약품개발 연구소)를 고온 페놀-물 추출방법에 의해 NMB-R6 세포로부터 추출하고, 초원심분리를 통해 정제하였다. Lipopolysaccharide (purchased from Chonnam National University Drug Research Institute) was extracted from NMB-R6 cells by high temperature phenol-water extraction and purified through ultracentrifugation.
구체적으로 정제 방법을 설명하면 다음과 같다. 세포 펠릿들을 5mM EDTA 및 0.02% 나트륨 아지드를 함유하는 포스페이트완충액에 현탁시킨 후, 리소자임을 2mg/ml 농도로 현탁액에 가한다. 이어서, 이 혼합물을 4℃에서 분해시킨 후 현탁액을 37℃로 되게 하고, 100ug/ml 농도의 RNAse 및 DNAse을 추가로 3시간 분해시킨다. 이어서 분해물을 70℃로 가열하고, 이에 페놀을 70℃에서 가한다. 이 혼합물을 15분의 기간 동안 추출하고, 현탁액을 4℃로 냉각시키고, 30분 동안 원심 분리한다. 수성상을 회수하고, 페놀상을 70℃에서 15분 동안 동량의 물로 재추출한다. 이후, 이 상을 30분 동안 원심분리하고, 수성상을 분리한다. 나트륨 아세테이트를 5mg/ml의 농도로 합하여 가한다. 이어서, 아세톤 3ml를 이 혼합물에 가하고, 리포폴리사카라이드를 4℃에서 침전시킨다. 침전된 리포폴리사카라이드를 30분동안 원심분리에 의해 분리한다. 전형적으로, 리포폴리사카라이드 3mg을 NMB-R6 세포 1mg 습윤중량으로부터 정제한다.Specifically, the purification method is as follows. Cell pellets are suspended in phosphate buffer containing 5 mM EDTA and 0.02% sodium azide and then lysozyme is added to the suspension at a concentration of 2 mg / ml. This mixture is then digested at 4 ° C. and the suspension is brought to 37 ° C. and RNAse and DNAse at 100 ug / ml concentration are further digested for 3 hours. The digest is then heated to 70 ° C., to which phenol is added at 70 ° C. The mixture is extracted for a period of 15 minutes, the suspension is cooled to 4 ° C. and centrifuged for 30 minutes. The aqueous phase is recovered and the phenol phase is reextracted with the same amount of water at 70 ° C. for 15 minutes. This phase is then centrifuged for 30 minutes and the aqueous phase is separated off. Sodium acetate is added in combination at a concentration of 5 mg / ml. Then 3 ml of acetone are added to this mixture and the lipopolysaccharide is precipitated at 4 ° C. Precipitated lipopolysaccharide is separated by centrifugation for 30 minutes. Typically, 3 mg lipopolysaccharide is purified from 1 mg wet weight of NMB-R6 cells.
<< 실험예Experimental Example 1>본 발명 혼합추출물의 항염증 효과 확인 1> Check the anti-inflammatory effect of the mixed extract of the present invention
1)실험방법1) Experiment Method
상기 추출물에 대하여 RAW 264.7 세포(구입처: 전남대학교 약품개발 연구소)를 페놀 레드가 없는 10% FBS가 함유된 동물세포배양 배지에 현탁하여 24 well plate의 각 well 당 5x105개씩 넣어 37℃, 5% CO2 배양 조건에서 24시간 동안 배양한다. RAW 264.7 세포에 리포폴리사카라이드와 식물 추출물 혹은 디메틸설폭사이드를 동시에 처리하여 20시간 배양 후 상등액 100㎕를 그리에스 시약 180㎕와 반응시켜 540㎚에서 흡광도를 측정한다.For the extract, RAW 264.7 cells (purchased from Chonnam National University Drug Research Institute) were suspended in animal cell culture medium containing 10% FBS without phenol red, and 5 × 10 5 of each well of a 24 well plate was put at 37 ° C., 5%. Incubate for 24 hours under CO 2 culture conditions. After treatment with lipopolysaccharide and plant extract or dimethyl sulfoxide simultaneously in RAW 264.7 cells, 100 μl of the supernatant was reacted with 180 μl of Gries reagent, and the absorbance was measured at 540 nm.
대조예1, 비교예1 내지 4 및 실시예1 내지 10 추출한 추출물을95%에탄올 1ml에 녹여1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, 1000 ㎍/㎖ 농도별로 상기 방법에 따라 항염증 실험을 하였다.Comparative Example 1, Comparative Examples 1 to 4 and Examples 1 to 10 The extracted extract was dissolved in 1 ml of 95% ethanol, and the concentration of 1 μg / ml, 10 μg / ml, 100 μg / ml, and 1000 μg / ml was determined according to the above method. Inflammation experiments were conducted.
2)실험결과2) Experimental results
항염효과는 계산식1에 의해 계산된 값이며, 그 결과를 표 4에 나타내었다.Anti-inflammatory effect is a value calculated by the formula 1, the results are shown in Table 4.
<< 계산식1Calculation 1 >>
상기 표4에 나타낸 바와 같이, 본 발명의 혼합추출물은 단일 성분 추출물(비교예1,2,3,4)이나 대조예1로 사용한 인도메타신보다 우수한 항염 효과가 있음이 확인되었다. As shown in Table 4, the mixed extract of the present invention was confirmed to have a superior anti-inflammatory effect than indomethacin used as a single component extract (Comparative Examples 1, 2, 3, 4) or Comparative Example 1.
<< 실험예Experimental Example 2> 본 발명의 혼합추출물의 항산화 효과 확인 2> Confirm the antioxidant effect of the mixed extract of the present invention
1)실험방법1) Experiment Method
0.2 mM DPPH 메탄올 용액 150㎕와 희석한 각각의 샘플 용액 150㎕를 각각 96-well plate에 넣고 상온에서 30분간 반응시킨 후 517㎚에서 흡광도를 측정한다. 샘플 150㎕와 메탄올 150㎕를 섞어서 블랭크(blank)로 하고 DPPH 메탄올 용액 150㎕와 메탄올 150㎕를 섞은 것을 대조군(control)로 하여 하기 계산식2에 따라서 항산화 활성을 계산한다.150 μl of 0.2 mM DPPH methanol solution and 150 μl of each diluted sample solution were put in a 96-well plate and reacted at room temperature for 30 minutes, and then absorbance was measured at 517 nm. 150 μl of sample and 150 μl of methanol were mixed to make a blank, and 150 μl of DPPH methanol solution and 150 μl of methanol were used as a control to calculate antioxidant activity according to the following Equation 2.
<< 계산식2Calculation 2 >>
(Sabs : 샘플의 흡광도, Babs : 블랭크의 흡광도, Cabs : 대조군의 흡광도)(S abs : absorbance of sample, B abs : absorbance of blank, C abs : absorbance of control)
상기 방법에 따라 대조예2 내지 5, 비교예1 내지 4 및 실시예1 내지 실시예10 에서 추출한 추출물을 메탄올 1ml에 녹여 1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, 1000 ㎍/㎖ 농도별로 항산화 실험을 하였다.According to the above method, the extract extracted in Comparative Examples 2 to 5, Comparative Examples 1 to 4 and Examples 1 to 10 was dissolved in 1 ml of methanol, and 1 µg / ml, 10 µg / ml, 100 µg / ml, and 1000 µg / ml. Antioxidant experiments were conducted for each concentration.
2)실험결과2) Experimental results
항산화 효과는 계산식2에 의해 나타내었고 그 결과를 표 5에 나타내었다. Antioxidant effect is shown by the formula 2 and the results are shown in Table 5.
상기 표5에 나타낸 바와 같이, 본발명의 혼합추출물은 단일 성분추출물(비교예1,2,3,4)이나 대조예2 내지 5의 추출물보다 우수한 항산화 효과 나타내었으며, 농도가 증가할수록 항산화 활성이 급격히 증가함을 알 수 있었다. 보다 구체적으로 혼합추출물(실시예1)의 경우 단일 성분 추출물에 비해 시너지 효과에 의해 더욱 가파른 상승곡선을 그리며 항산화 활성이 증가하였고, 대조군인 녹차추출물(대조예2)에 비해서도 높은 항산화 활성을 갖고 있음이 확인되었다. As shown in Table 5, the mixed extract of the present invention showed a superior antioxidant effect than a single component extract (Comparative Examples 1, 2, 3, 4) or the extracts of Comparative Examples 2 to 5, and the antioxidant activity was increased with increasing concentration. It was found to increase rapidly. More specifically, in the case of the mixed extract (Example 1), the antioxidant activity was increased by a synergistic effect compared to the single component extract, and the antioxidant activity was increased, and it had a higher antioxidant activity than the green tea extract (Control 2), which was the control group. This was confirmed.
<< 실험예3Experimental Example 3 > 본 발명의 혼합추출물의 안전성 시험> Safety test of the mixed extract of the present invention
1)실험방법1) Experiment Method
상기 추출물에 대하여 안전성 실험을 하기 위하여 실시예1를 사용하였다. Human fibroblast cell line(인간 섬유아세포)을 1x103 cell/well의 농도로 접시에 파종 한다. 10% FBS가 함유된 동물세포배양 배지를 이용하여 37℃, 5% CO2 배양 조건에서 24시간 동안 배양한다. 각각의 추출물을 디메틸설폭사이드에 녹여 배지와 혼합한 후 200㎕씩 로딩 한 후 37℃, 5% CO2 배양 조건에서 72시간 동안 배양한다. 배양이 끝난 후 배지 200㎕에 MTT 용액 50㎕를 로딩하여 5시간 배양한다. 배양 후 MTT 용액을 제거한 다음 디메틸설폭사이드 200㎕를 넣고 540㎚에서 흡광도를 측정한다.Example 1 was used to perform a safety experiment on the extract. Human fibroblast cell lines are seeded in a dish at a concentration of 1 × 10 3 cells / well. Incubate for 24 hours at 37 ° C., 5% CO 2 culture conditions using animal cell culture medium containing 10% FBS. Each extract was dissolved in dimethyl sulfoxide, mixed with the medium, loaded with 200 μl, and then incubated for 72 hours at 37 ° C. and 5% CO 2 culture conditions. After incubation, 50 μl of MTT solution was loaded into 200 μl of medium and incubated for 5 hours. After incubation, the MTT solution was removed, and then 200 µl of dimethyl sulfoxide was added, and the absorbance was measured at 540 nm.
상기 방법에 따라 실시예1 및 대조예2에서 추출한 추출물을 95%에탄올 1ml에 녹여 1㎖, 10 ㎍/㎖, 100 ㎍/㎖, 1000 ㎍/㎖ 농도별로 안전성을 측정하였다.According to the above method, the extracts extracted in Example 1 and Comparative Example 2 were dissolved in 1 ml of 95% ethanol, and the safety was measured for each concentration of 1 ml, 10 µg / ml, 100 µg / ml, and 1000 µg / ml.
2)실험결과2) Experimental results
실험예3의 방법에 따른 결과를 표 6에 나타내었다.Table 6 shows the results according to the method of Experimental Example 3.
일반적으로 천연추출물의 농도를 증가함에 따라 안정성은 미미하게 떨어지지만 실시예1의 안전성 효과는 떨어지지 않음을 볼 수 있다. 따라서 본 발명의 혼합추출물은 기존 녹차추출물(대조예2)과 비교하여 안전성면에서 현저히 우수함을 알 수 있다.In general, as the concentration of the natural extract is increased, the stability is slightly reduced, but it can be seen that the safety effect of Example 1 is not degraded. Therefore, it can be seen that the mixed extract of the present invention is significantly superior in terms of safety compared to the conventional green tea extract (Control Example 2).
<< 실험예Experimental Example 4> 본 발명의 혼합추출물의 피부자극 완화 효과 확인 4> Confirm the skin irritation relief effect of the mixed extract of the present invention
1)실험방법1) Experiment Method
누드 마우스[BALB/C sic-nu/nu mouse(12-17g, ♀)]에 증류수에 0.5%로 희석한 컴파운드 48/80(피부자극 유도물질)을 오른쪽 어깨에 주사한 후 1 시간 동안 긁는 횟수를 측정한다. 그 다음 24시간 후에 12마리의 쥐에 표 7의 화장료 처방예 1 내지 6를 각각 100 ul를 왼쪽 어깨에 주사하고 10분 뒤에 동일한 자리에 0.5% 컴파운드 48/80을 50 ul 주사하여 한 시간 동안 긁는 횟수를 측정한다. 0.5%의 컴파운드 48/80만을 주사하였을 때의 긁는 횟수를 100%로 환산하여 기준공통 횟수로 하고 이에 대하여 시료 및 컴파운드 48/80을 주사한 후 긁은 횟수를 공통기준횟수에 비교하여 나타내었다.Number of scratches for 1 hour after injection of BALB / C sic-nu / nu mouse (12-17g, ♀) into right shoulder with Compound 48/80 (skin irritation inducer) diluted in distilled water at 0.5% Measure After 24 hours, 12 rats were injected with 100 ul of each of the cosmetic formulations 1 to 6 of Table 7 on the left shoulder, and 10 minutes later, 50 ul of 0.5% compound 48/80 was injected in the same place and scraped for 1 hour. Measure the number of times. The number of scratches when only 0.5% of compound 48/80 was injected was converted to 100%, and the number of scratches after injection of the sample and compound 48/80 was compared with the common reference frequency.
2) 처방예1 내지 6의 제조2) Preparation of Prescription Examples 1-6
성분15, 16, 17, 19번을 혼합믹서기에 넣고 혼합 교반하면서 80~85℃사이로 가열하여 제조부에 투입한 후 유화기를 작용시키고 성분 7, 8, 9, 10, 11, 12, 13, 14번을 80~85℃사이로 가열하여 용해한 후 성분18번을 투입 교반하여 제조부에 투입하고 유화시킨다. 유화가 끝나면 교반기를 이용하여 교반하면서 35℃까지 냉각하고 성분1, 2, 3, 4, 5 및 6번을 투입하여 25℃까지 냉각한 뒤 숙성시켜 처방예1 내지 6을 제조하였다.Put ingredients 15, 16, 17, and 19 into the mixing mixer, heat the mixture to 80 ~ 85 ℃ while mixing and stirring, put into the manufacturing unit, and then work the emulsifier, and the components 7, 8, 9, 10, 11, 12, 13, 14 After dissolving by heating to 80 ~ 85 ℃ to dissolve component No. 18 is added to the manufacturing unit and emulsified. After the emulsification was completed by stirring using a stirrer to cool to 35 ℃ and the ingredients 1, 2, 3, 4, 5 and 6 was added to cool to 25 ℃ and aged to prepare Formulation Examples 1-6.
3)실험결과3) Experimental results
실험예4의 실험 방법을 3번 반복하여 그 평균치를 하기 표8 에 나타내었다. The experimental method of Experiment 4 was repeated three times and its average is shown in Table 8 below.
이 실험 결과로부터 본 발명의 혼합물추출물은 단일 성분의 추출물에 비해 우수한 피부자극완화 효과가 있음이 확인되었다. From the results of this experiment, the mixture extract of the present invention was confirmed to have an excellent skin irritation relaxing effect compared to the extract of a single component.
<< 실험예Experimental Example 5> 본 발명의 혼합추출물의 보습 효과 확인 5> Check the moisturizing effect of the mixed extract of the present invention
1) 실험방법1) Experiment Method
본 발명의 혼합추출물 및 Buthylene Glycol(대조군) 5㎖를 각각 plate에 넣은 후 완전 건조 된 CaCl2를 흡습제로 하여 35℃ 데시케이터에서 무게를 측정하였다. 무게는 총 144시간 동안 측정하였다. 그 결과를 표 9에 나타내었다.The mixed extract of the present invention and 5 ml of Buthylene Glycol (control) were put in a plate, respectively, and the weight was measured in a 35 ° C. desiccator using completely dried CaCl 2 as an absorbent. The weight was measured for a total of 144 hours. The results are shown in Table 9.
상기 결과로부터 본 발명의 혼합추출물이 기존 Buthylene Glycol과 비교하여 보습력에서 현저히 우수한 효과가 있음이 확인되었다.From the results, it was confirmed that the mixed extract of the present invention has a remarkably superior effect in moisturizing power compared to the conventional buthylene glycol.
<< 제제예Formulation example 1> 1> 본발명이The present invention 혼합추출물을 함유하는 화장수 제조 Preparation of lotion containing mixed extract
실시예1의 혼합추출물을 함유한 화장수 (스킨로션) 1kg을 하기 표의 조성으로 다음과 같은 방법으로 제조하였다. 1 kg of the lotion (skin lotion) containing the mixed extract of Example 1 was prepared by the following method with the composition of the following table.
성분 11번에 성분 2,3,4 및 8번을 혼합믹서기에 순서대로 투입하고 실온에서 교반하여 용해시켰다. 그 후, 성분 5번을 60℃ 정도로 가열하여 용해시킨 후, 성분 10번을 투입, 교반하여 용해한 후 성분 11번에 투입하였다. 마지막으로 성분 1,6,7 및 9번을 투입하여 충분히 실온에서 교반한 뒤 72시간 숙성시켜 표제의 화장수를 제조하였다.Components 2, 3, 4 and 8 were added to component 11 in the mixing mixer in order, and stirred at room temperature to dissolve. Thereafter, component No. 5 was heated to about 60 ° C. for dissolution, and then component No. 10 was added and stirred to dissolve, followed by ingredient 11. Finally, ingredients 1, 6, 7, and 9 were added thereto, sufficiently stirred at room temperature, and aged for 72 hours to prepare the title lotion.
<< 제제예Formulation example 2> 2> 본발명의Invention 혼합추출물을 함유하는 영양로션 제조 Preparation of nutrition lotion containing mixed extract
실시예1의 혼합추출물을 함유한 영양로션 1kg을 하기 표의 조성으로 제조하였다.1 kg of nutrient lotion containing the mixed extract of Example 1 was prepared in the composition of the following table.
성분 2, 3, 4, 5, 및 6번을 혼합믹서기에 투입하고 실온에서 균질화하고, 이에 성분1, 7, 8 및 14번을 혼합하고, 실온에서 균질화 한 후 성분 9번을 실온에서 서서히 첨가하여 균질화시켰다. 그 후, 성분 10, 11, 12, 13번을 투입하여 분산시킨 후, 실온에서 안정화한 다음 72시간 숙성시켜 표제의 영양로션을 제조하였다.Add components 2, 3, 4, 5, and 6 to the mixer and homogenize at room temperature, mix components 1, 7, 8 and 14 thereafter, homogenize at room temperature and add component 9 slowly at room temperature Homogenized by. Thereafter, components 10, 11, 12, and 13 were added to disperse the mixture, stabilized at room temperature, and aged for 72 hours to prepare the title nutrition lotion.
<< 제제예Formulation example 3> 3> 본발명의Invention 혼합추출물을 함유하는 영양크림 제조 Preparation of nutrition cream containing mixed extract
실시예1의 혼합추출물을 함유한 영양크림 1kg을 하기 표의 조성으로 제조하였다.1 kg of nourishing cream containing the mixed extract of Example 1 was prepared in the composition of the following table.
성분 2, 3, 4, 5 및 6번을 실온에서 균질화한 비이온계 양친매성 지질과 성분 1, 7, 8, 및 14번를 혼합믹서기에서 혼합하고, 실온에서 균질화 한 후 성분 9번을 실온에서 서서히 첨가하여 균질화하였다. 그 후, 성분 10, 11, 12, 13번을 투입하여 분산시켜 실온에서 안정화한 다음 72시간 숙성시켜 표제의 영양크림을 제조하였다.Nonionic amphiphilic lipids homogenized components 2, 3, 4, 5, and 6 at room temperature and components 1, 7, 8, and 14 were mixed in a mixer, and homogenized at room temperature. Add slowly and homogenize. Then, ingredients 10, 11, 12, and 13 were added to disperse, stabilized at room temperature, and aged for 72 hours to prepare the nutrient cream of the title.
<< 제제예Formulation example 4> 본 발명의 혼합추출물을 함유하는 에센스 제조 4> Preparation of essence containing the mixed extract of the present invention
실시예 1의 혼합추출물을 함유한 에센스 1kg을 하기 표의 조성으로 제조하였다.1 kg of the essence containing the mixed extract of Example 1 was prepared in the composition of the following table.
성분 2, 3, 4, 5 및 6번을 실온에서 균질화한 비이온계 양친매성 지질과 성분 1, 7, 8 및 15번을 혼합 믹서에서 혼합하고 실온에서 균질화하여 마이크로플루이다이져를 통과하고 이어 성분 9번을 실온에서 서서히 첨가하여 균질화한 후 다시 마이크로플루이다이져에 재차 통과시켰다. 그리고 성분 10, 11, 12, 13 및 14번을 투입하여 분산시켜 실온에서 안정화하고 72시간 숙성시켜 표제의 에센스를 제조하였다.Nonionic amphiphilic lipids homogenized components 2, 3, 4, 5 and 6 at room temperature and components 1, 7, 8 and 15 were mixed in a mixing mixer and homogenized at room temperature to pass through a microfluidizer and then Component 9 was slowly added to homogenize at room temperature and passed again through a microfluidizer. The ingredients 10, 11, 12, 13 and 14 were added and dispersed, stabilized at room temperature, and aged for 72 hours to prepare the title essence.
도1은 본 발명의 혼합추출물의 제조 방법의 모식도를 나타낸 도 및 사진이다. 1 is a view and a photograph showing a schematic diagram of a method for producing a mixed extract of the present invention.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101156636B1 (en) * | 2010-12-01 | 2012-06-14 | 동국대학교 경주캠퍼스 산학협력단 | Pharmaceutical composition comprising an extract of Smilax china L. for preventing and treating blood vessel disease |
| KR101479902B1 (en) * | 2013-06-17 | 2015-01-08 | 세종대학교산학협력단 | Method for manufacturing Smilax China L root extract having increased active ingredients and beverage composition containing the extract for detoxification |
| KR20160126771A (en) * | 2015-04-24 | 2016-11-02 | 주식회사 참 존 | Composition for reducing skin wrinkle and for promoting recovery of iskin injury comprising extracts of Orostachys japonicus and Saururus chinensis |
| KR101867386B1 (en) * | 2012-03-23 | 2018-06-15 | (주)아모레퍼시픽 | Composition containing natural extracts |
| CN111686061A (en) * | 2020-07-31 | 2020-09-22 | 中科瑞晟芳香产业研究院(湖北)有限公司 | Composition with skin whitening and moisturizing effects and using method |
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| KR101835356B1 (en) * | 2015-10-30 | 2018-03-08 | (주)튜링겐코리아 | Method for producing extract of Selaginella tamariscina with enhanced extraction yield using ultra high-pressure homogenization |
| KR102072111B1 (en) * | 2017-11-29 | 2020-01-31 | 대구대학교 산학협력단 | Antioxidant composition comprising defatted perilla extract as effective component and manufacturing method thereof |
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| JP3987793B2 (en) * | 2002-12-27 | 2007-10-10 | 株式会社コーセー | Topical skin preparation |
| KR20040092496A (en) * | 2003-04-24 | 2004-11-04 | 김정태 | Dried laver food added chiness medicinal herbs and the processing method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101156636B1 (en) * | 2010-12-01 | 2012-06-14 | 동국대학교 경주캠퍼스 산학협력단 | Pharmaceutical composition comprising an extract of Smilax china L. for preventing and treating blood vessel disease |
| KR101867386B1 (en) * | 2012-03-23 | 2018-06-15 | (주)아모레퍼시픽 | Composition containing natural extracts |
| KR101479902B1 (en) * | 2013-06-17 | 2015-01-08 | 세종대학교산학협력단 | Method for manufacturing Smilax China L root extract having increased active ingredients and beverage composition containing the extract for detoxification |
| KR20160126771A (en) * | 2015-04-24 | 2016-11-02 | 주식회사 참 존 | Composition for reducing skin wrinkle and for promoting recovery of iskin injury comprising extracts of Orostachys japonicus and Saururus chinensis |
| CN111686061A (en) * | 2020-07-31 | 2020-09-22 | 中科瑞晟芳香产业研究院(湖北)有限公司 | Composition with skin whitening and moisturizing effects and using method |
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