KR20060112408A - Culture dish with comparted-cells using same medium - Google Patents
Culture dish with comparted-cells using same medium Download PDFInfo
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- KR20060112408A KR20060112408A KR1020050034816A KR20050034816A KR20060112408A KR 20060112408 A KR20060112408 A KR 20060112408A KR 1020050034816 A KR1020050034816 A KR 1020050034816A KR 20050034816 A KR20050034816 A KR 20050034816A KR 20060112408 A KR20060112408 A KR 20060112408A
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- 238000005192 partition Methods 0.000 claims abstract description 30
- 238000012258 culturing Methods 0.000 claims abstract 2
- 238000000638 solvent extraction Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 238000009792 diffusion process Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60S—SERVICING, CLEANING, REPAIRING, SUPPORTING, LIFTING, OR MANOEUVRING OF VEHICLES, NOT OTHERWISE PROVIDED FOR
- B60S3/00—Vehicle cleaning apparatus not integral with vehicles
- B60S3/04—Vehicle cleaning apparatus not integral with vehicles for exteriors of land vehicles
- B60S3/042—Wheel cleaning devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60S—SERVICING, CLEANING, REPAIRING, SUPPORTING, LIFTING, OR MANOEUVRING OF VEHICLES, NOT OTHERWISE PROVIDED FOR
- B60S3/00—Vehicle cleaning apparatus not integral with vehicles
- B60S3/04—Vehicle cleaning apparatus not integral with vehicles for exteriors of land vehicles
- B60S3/06—Vehicle cleaning apparatus not integral with vehicles for exteriors of land vehicles with rotary bodies contacting the vehicle
- B60S3/066—Vehicle cleaning apparatus not integral with vehicles for exteriors of land vehicles with rotary bodies contacting the vehicle the axis of rotation being approximately horizontal
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B1/00—Cleaning by methods involving the use of tools
- B08B1/30—Cleaning by methods involving the use of tools by movement of cleaning members over a surface
- B08B1/32—Cleaning by methods involving the use of tools by movement of cleaning members over a surface using rotary cleaning members
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
도면은 일반배양기와 본 출원의 발명품인 칸막이가 설치된 배양용기 및 칸막이에 대해 나타내었다. 칸막이의 다리부분과 배양용기의 아래쪽 윗면이 만들어내는 공극을 통해 배지가 다리윗부분까지 확산되도록 만들어졌다.The drawings are shown for the culture vessel and the partition installed in the general culture and the partition of the invention of the present application. The medium spreads to the upper leg through the pores created by the leg of the partition and the lower surface of the culture vessel.
1a : 일반배양용기의 측면도 1b : 일반배양용기의 전면도 1a: Side view of general culture container 1b: Front view of general culture container
2a : 칸막이가 사용된 배양용기의 측면도 2a: side view of a culture vessel in which a partition is used
2b : 칸막이가 사용된 배양용기의 전면도 2b: Front view of culture vessel with partition
3a : 배지를 공유하는 칸막이의 측면도 3a: side view of a divider sharing badge
3b : 배지를 공유하는 칸막이의 측면도 3b: side view of dividers sharing badge
본 발명은 생물공학에 널리 쓰이는 배양용기에 있어 균 등에 오염된 재료를 배양하였을 때 하나의 오염원 때문에 나머지 전체가 모두 못쓰게 되는 것을 방지하고자 중간에 칸막이를 설치하여 오염된 균등이 최초의 오염체로부터 다른 오염되지 않은 재료로 옮겨가는 것을 방지하는 방법에 관한 것이다. 이러한 방지는 용기를 별도로 사용하거나 칸막이를 해야 가능하지만, 이 경우 배지를 만드는데 많은 노력과 공간 등이 소요되는데, 본 발명에서는 배지를 공유하는 칸막이를 도입하여 하나의 용기를 칸막이를 하면서도 배지조제에 있어 시간이나 공간을 획기적으로 절약할 수 있는 배양용기와 그 제작방법에 대한 것이다.The present invention is a culture vessel widely used in biotechnology, when cultivated the contaminated material in bacteria, etc. to prevent the entire remaining of the entire waste due to one contamination source to install a partition in the middle of the contaminated evenly different from the first contaminant A method of preventing transfer to an uncontaminated material. Such prevention is possible by using a separate container or partitioning, but in this case, it takes a lot of effort and space to make a badge, in the present invention by introducing a partition sharing a medium to divide one container while preparing a medium It is about a culture vessel and its manufacturing method that can save a significant amount of time or space.
배양용기의 고체배지의 성형 방법에 관한 것으로, 더욱 상세하게는 배양용기에 칸막이를 설치하여 균의 이동을 막음과 동시에 다리를 설치하여 아래쪽에 공간을 부여하여 배지를 채우거나 담기 용이함과 동시에 배지의 성형을 통해 칸막이를 완성시켜 오염을 막을 수 있는 배지 성형방법에 관한 것이다.The present invention relates to a method of forming a solid medium of a culture vessel. More specifically, a partition is installed in a culture vessel to prevent bacteria from moving, and a leg is provided to provide a space at the bottom to easily fill or contain a medium. It relates to a medium forming method that can prevent the contamination by completing the partition through the molding.
일반적으로 오염체를 구분 배양하기 위해서는 시험관을 이용하여 하나하나 분리하여 배양하거나 오염을 감수하고 한꺼번에 배양용기에 배양하고 있으며, 칸막이가 되어 있는 배양용기류도 있으나 매 칸마다 배지를 넣기 위해 개별 공정이 필요하거나 특수한 기구를 사용하고 있다.In general, in order to classify contaminants, the test tubes are used to separate them one by one, or they are cultured in a culture vessel at a time, and there is a partitioned culture vessel, but a separate process is required for each medium. Or special equipment is used.
생물배양은 생물공학의 시발점이며, 이는 무균상태의 배양체를 확보하는 데에서 시작되는데, 무균상태의 배양체의 획득은 오염된 개체에서 살균조작을 통해 무균개체를 획득해내야 하며, 이 무균 개체들 사이에서 어느 것이 실제로 무균상태인가를 확인하는 것은 결국 배양을 통해 이뤄지게 되는데 이 초기 배양에서의 오염개체와 무균개체를 구분하기 위한 배양에서 시험관과 같이 별도용기의 사용, 또는 배양접시나 배양병을 이용한 배양방법 중 하나가 쓰이며, 전자는 배지조제를 일일이 하나하나 만들어야 하는 단점이 있으며, 후자는 배양병이나 접시내에 오염개체가 생기면 그 용기내의 모든 개체들이 비록 증상은 없어도 이미 배양기간중에 오염된 결과가 많았다. 칸막이가 되어 있어 작은 방을 가진 배양접시가 있으나 이 배양접시는 배지를 만드는데 있어 각 방마다 배지를 별도로 만들어서 오랜 시간이 걸리거나 고가의 설비가 필요하여 사용에 불편하였으며 결과적으로 생물 배양을 지체시키는 요인의 하나가 되어 있다.Bioculture is the starting point of biotechnology, which begins with the acquisition of sterile cultures. The acquisition of sterile cultures requires the sterilization of contaminated individuals to obtain sterile objects. The identification of which is actually sterile at the end is accomplished through cultivation. In the initial cultivation, a separate container such as a test tube is used to distinguish between contaminated and sterile objects, or culture using a culture dish or a culture bottle. One of the methods is used, the former has a disadvantage in that each medium is prepared one by one, the latter is a contaminated object in a culture bottle or a dish, all the individuals in the container, although there are no symptoms, many of the results were already contaminated during the culture period. . Although there is a culture plate with a small room because it is partitioned, it is inconvenient to use because it takes a long time or requires expensive facilities to make a medium in each room. There is one.
본 발명은 상기와 같은 문제점을 해소하기 위해 다리가 있는 칸막이를 도입하였으며, 이 다리가 있는 칸막이를 통해 배양용기내 공간을 구분할 수 있음과 동시에 다리부분에서 만들어진 공간을 통해 배지를 전면 즉 모든 칸막이가 배지를 공유하므로서, 배지를 담고 채우는데 간편성을 부여하였으며, 배지가 고형화 될 경우 칸막이와 용기의 두껑 그리고 배지표면이 각각 용기내의 공간들을 독립된 공간을 형성시키게 되며 이를 통해 균의 이동을 막는 것에 특징이 있다. 이를 통해 생물공학의 시작단계인 무균 시발체의 확보가 용이하게 될 수 있다.The present invention has introduced a partition with a partition to solve the above problems, the partition can be divided into the space in the culture vessel through the partition at the same time through the space made in the leg portion, that is, all the partitions By sharing the medium, it provides simplicity in storing and filling the medium, and when the medium is solidified, the partition, the lid of the container, and the surface of the medium form separate spaces in the container, thereby preventing the movement of bacteria. have. Through this, it is easy to secure sterile primers, which are the beginning stages of biotechnology.
이하 첨부된 도면에 의해 상세히 설명하면 다음과 같다.Hereinafter, described in detail by the accompanying drawings as follows.
도 1은 기존에 사용되고 있는 배양용기로서 전체가 하나의 공간으로 구성되어 있다. 도3는 본 발명에 사용된 칸막이로서 다리를 가지며 전체 높이는 아래접시의 내벽과 같아서 두껑과 밀착되어 있다. 칸막이는 아래접시에 넣어져 설치되며, 이곳에 배지를 담으면 다리에 의해 아래접시의 윗표면과 칸막이의 아래면과 사이에 다리만큼의 공간이 생기며 이를 통해 유동성의 배지가 확산되며, 배지의 양을 조절하면 칸막이의 아래면을 지나 채워져 우물을 형성하게 되며, 두껑의 아래면과 칸막이의 윗면이 밀착되므로 우물은 다시 방을 형성하게 된다. 이와 같이 하여 칸막이가 만드는 각각의 칸은 하나의 독립된 방을 형성하며, 이를 이용하면 방안에 넣어진 생물재료들은 오염된 것과 무균인 것들이 구분되어 생장하게 되며, 이를 일정기간 배양한 후 오염되지 않은 것만을 선별적으로 골라낼 수 있으므로 생물공학의 시발물질인 무균배양체의 획득이 용이하게 된다. 1 is a culture vessel used in the prior art is composed of a whole space. Figure 3 is a partition used in the present invention has a leg and the overall height is in close contact with the lid as the inner wall of the lower plate. The partition is placed in the bottom plate, and if you put the badge in it, the space between the top surface of the bottom plate and the bottom of the partition and the space is spread by the legs. If you adjust it to fill the bottom of the partition to form a well, the bottom of the lid and the top of the partition is in close contact with the well to form a room again. In this way, each compartment created by the divider forms an independent room, and when this is used, the biological materials in the room are separated from the contaminated and sterile ones, and grow only after being incubated for a certain period of time. Since it is possible to select selectively, it is easy to obtain a sterile culture that is a starting material of biotechnology.
1a : 일반배양용기의 측면도 1b : 일반배양용기의 전면도 1a: Side view of general culture container 1b: Front view of general culture container
2a : 칸막이가 사용된 배양용기의 측면도 2a: side view of a culture vessel in which a partition is used
2b : 칸막이가 사용된 배양용기의 전면도 2b: Front view of culture vessel with partition
3a : 배지를 공유하는 칸막이의 측면도 3a: side view of a divider sharing badge
3b : 배지를 공유하는 칸막이의 측면도 3b: side view of dividers sharing badge
이상에서 상술한 바와 같이 본 발명은, 생물자원 배양시 오염개체와 무균개체의 구분, 또는 서로 다른 균의 배양, 또는 서로 다른 생물체나 세포 등을 함께 만든 배지에서 배양하고자 할 때 배지조제의 용이성은 물론, 배양공간의 절약, 배지조제 시간이나 노력, 설비의 절감 등의 장점을 가질 수 있는 것이다.As described above, in the present invention, when the biological resources are cultivated to distinguish between the contaminated and aseptic individual, or to culture the different bacteria, or to culture in a medium made with different organisms or cells, the ease of preparation of the medium Of course, it can have advantages such as saving of the culture space, preparation time or effort of the medium, saving of equipment.
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KR100827755B1 (en) * | 2007-03-30 | 2008-05-07 | 소창호 | Cultivation dish |
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KR101422345B1 (en) | 2012-10-19 | 2014-07-22 | 한국생명공학연구원 | Dual structured cell culture dish |
US11732232B2 (en) | 2020-08-14 | 2023-08-22 | Korea Research Institute Of Bioscience And Biotechnology | Biomimetic cell culture apparatus and cell culture system comprising the same |
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