JP2627142B2 - Fractionation medium and method for producing the same - Google Patents
Fractionation medium and method for producing the sameInfo
- Publication number
- JP2627142B2 JP2627142B2 JP6121928A JP12192894A JP2627142B2 JP 2627142 B2 JP2627142 B2 JP 2627142B2 JP 6121928 A JP6121928 A JP 6121928A JP 12192894 A JP12192894 A JP 12192894A JP 2627142 B2 JP2627142 B2 JP 2627142B2
- Authority
- JP
- Japan
- Prior art keywords
- medium
- fractionation
- petri dish
- partition
- partition wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、微生物の培養、検出、
分離、同定等に有用な培地が容器内に分画された状態で
注入されている分画培地に関する。The present invention relates to the cultivation and detection of microorganisms,
The present invention relates to a fractionation medium into which a medium useful for separation, identification, and the like is fractionated and injected.
【０００２】[0002]
【従来の技術】微生物学的な検査、実験等においては、
数多くの検体について微生物の培養、検出、分離、同定
等の作業を行なうのにシャーレ、培養ビン、培養管等の
容器が使用されている。この作業に一つの容器内に一種
類の培地を注入したものを使用することは、多数個の容
器を必要とするため作業性に劣るので、省力化を図る目
的のために、一つの容器内に隔壁を設けて容器内部を複
数の区画に分画して、各々に分画された区画に培地を注
入して、微生物学的な検査、実験が行なわれている。こ
のような容器内の分画培地にエーゼ（釣菌子）等を用い
て微生物を接種するのに、複数の培地間に隔壁が突出し
ていると、エーゼの先端が引っ掛かり、接種作業に支障
をきたすので、培地表面と隔壁の上端部は同一水平面と
なるような試みがなされていた。2. Description of the Related Art In microbiological tests and experiments,
2. Description of the Related Art Containers such as petri dishes, culture bottles, culture tubes, and the like are used to perform operations such as culture, detection, separation, and identification of microorganisms on a large number of specimens. The use of a medium in which one type of medium is injected into one container for this work requires a large number of containers and is inferior in workability. The inside of the container is fractionated into a plurality of compartments, and a medium is injected into each compartment to conduct microbiological examination and experiment. When a microorganism is inoculated into a fractionation medium in such a container using ace (bacterial fungus) or the like, if a partition wall protrudes between a plurality of culture media, the tip of the ase is caught and hinders the inoculation work. Therefore, an attempt has been made to make the surface of the culture medium and the upper end of the partition wall the same horizontal plane.
【０００３】従来、例えば、培地の表面となる部分に平
坦な封止体を隔壁頂端に密着させて、シャーレの外側壁
の隔壁近傍に設けた注入孔から培地を一つの分画室へ注
入し、その注入口から気泡を放出させ、次いでその注入
孔を滅菌シール等で封止した後、シャーレを適宜移動
（回転）させて、別の空の分画室の注入孔に培地を注入
し、上記と同様な操作を行なって別の分画室へ培地の充
填を完了させることにより、培地表面と隔壁の上端部が
同一水平面となるように培地入分画シャーレを製造して
いた。そして、そのシャーレ内の培地に接種を行なう際
には、封止体を剥離した後に、エーゼ等で微生物を接種
していた（例えば、実公平５−３６４００号公報参
照）。また、同様に培地表面と隔壁の上端が同一水平面
となるようにするため、分画シャーレ内への培地の充填
時にシャーレの底部に設けた小孔から注入する方法も提
案されていた（例えば、特公平５−５８７０５号公報参
照）。[0003] Conventionally, for example, a flat sealing body is brought into close contact with the top end of a partition wall on a portion to be the surface of the culture medium, and the culture medium is injected into one fractionation chamber from an injection hole provided near the partition wall on the outer wall of the petri dish. After releasing air bubbles from the inlet and sealing the inlet with a sterile seal or the like, the petri dish is appropriately moved (rotated) to inject the medium into the inlet of another empty compartment, and A similar operation was performed to complete the filling of the culture medium into another compartment, whereby a medium-mixing petri dish was manufactured so that the surface of the culture medium and the upper end of the partition wall were on the same horizontal plane. Then, when inoculating the medium in the petri dish, the microorganism was inoculated with Ese or the like after peeling off the sealed body (for example, see Japanese Utility Model Publication No. 5-36400). Similarly, a method has been proposed in which the medium surface and the upper end of the partition wall are in the same horizontal plane so that the medium is filled into a fractionation Petri dish through a small hole provided at the bottom of the Petri dish when filling the Petri dish (for example, Japanese Patent Publication No. 5-58705).
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、前記従
来の、培地の表面となる部分に平坦な封止体を配置し、
シャーレの外側壁の注入孔から培地を注入した培地入分
画シャーレの製造方法においては、一つの分画室へ培地
を注入し、注入孔を封止した後でないと、他の分画室へ
の注入が開始できないという不都合があった。また、前
記従来の培地入分画シャーレに微生物を接種する際に
は、シール等の平坦な封止体を各シャーレについて一枚
一枚剥離しなければならず、このため大量のシャーレに
対し接種作業を行なう際や、接種作業を機械によって行
なう際には、この封止体の剥離作業という余分な操作が
伴っていた。また、前記従来の培地入分画シャーレは、
培地表面と隔壁頂端とが同じ高さであり、このような培
地に対して微生物をエーゼ等により接種する際には、培
地は寒天等の弾力性物質のために多少エーゼの先端の圧
力により窪んだ状態でエーゼが移動されるが、隔壁部分
は、ガラス、プラスチック等の剛性部材のため、エーゼ
がこの部分を通過する時は多少引っ掛かりやすいという
問題があった。However, a flat sealing body is disposed on a portion of the conventional medium which will be the surface of the culture medium.
In the method for producing a medium-filled Petri dish in which the medium is injected from the injection hole on the outer wall of the Petri dish, the medium is injected into one compartment, and the injection into the other compartment is performed only after the injection hole is sealed. There was a problem that could not be started. In addition, when inoculating microorganisms into the conventional medium-fractionated petri dish, flat sealing bodies such as seals must be peeled off one by one for each petri dish, so that a large amount of petri dishes are inoculated. When performing the work or performing the inoculation work using a machine, an extra operation of peeling off the sealing body is involved. In addition, the conventional culture medium fractionation Petri dish,
The surface of the medium and the top of the partition wall are at the same height, and when inoculating a microorganism with such a medium using an enzyme or the like, the medium may be somewhat dented due to pressure on the tip of the enzyme due to an elastic substance such as agar. Although the ace is moved in a state where the ace is moved, the partition portion is a rigid member such as glass or plastic.
【０００５】一方、分画シャーレ内へ培地を充填するの
にシャーレの底部に設けた分注孔から注入する前記従来
の方法においては、シャーレの底部が水平であるため
に、分注孔周辺の培地に気泡が残留しやすいという欠点
があった。On the other hand, in the conventional method in which a medium is filled into a fractionation petri dish through a dispensing hole provided at the bottom of the petri dish, the bottom of the petri dish is horizontal. There is a disadvantage that air bubbles are likely to remain in the medium.
【０００６】そこで本発明は、微生物学的な検査、実験
が省力化できる分画培地であって、しかも、分画容器の
各分画室への培地の注入を同時に行なうことが可能であ
り、培地の表面には平坦な封止体が必要ではなく、した
がって、微生物の接種時には該封止体を剥離する作業が
なく、微生物の接種時にエーゼ等の接種手段の移動がス
ムーズに行なうことができ、さらに、培地内に気泡が残
留する問題のない分画培地を提供することを目的とす
る。Accordingly, the present invention is directed to a fractionation medium which can save labor for microbiological examinations and experiments, and can simultaneously inject the culture medium into each compartment of the fractionation container. There is no need for a flat sealing body on the surface.Therefore, there is no work of peeling off the sealing body at the time of inoculation of microorganisms, and the movement of inoculation means such as Ese can be smoothly performed at the time of inoculation of microorganisms. It is another object of the present invention to provide a fractionation medium free from bubbles remaining in the medium.
【０００７】[0007]
【課題を解決するための手段】前記した問題点を解決す
るために本発明の分画培地は、内部に隔壁を有し複数の
区画に分画されている分画容器の各区画内に培地が注入
されている分画培地において、該培地表面の高さが、前
記隔壁の高さよりも高いことを特徴とする。本発明の分
画培地においては、前記分画容器内の壁面と前記培地と
の間の剥離を防止するために、前記分画容器内には培地
の剥離防止手段が設けられていることを特徴とする。こ
の剥離防止手段は、分画容器内の隔壁近傍、側壁近傍の
底部及び／又は隔壁部に設けられた突起物であることを
特徴とする。Means for Solving the Problems In order to solve the above-mentioned problems, a fractionation medium of the present invention comprises a medium in each compartment of a fractionation container having a partition therein and divided into a plurality of compartments. Wherein the height of the surface of the culture medium is higher than the height of the partition walls. In the fractionation medium of the present invention, in order to prevent detachment between the wall surface in the fractionation container and the culture medium, a separation prevention means for the culture medium is provided in the fractionation container. And The separation preventing means is characterized in that it is a projection provided in the vicinity of the partition wall in the fractionation container, the bottom near the side wall, and / or the partition wall portion.
【０００８】また本発明の分画培地の製造方法は、内部
に隔壁を有し複数の区画に分画されている分画容器を用
意し、前記分画容器の各区画内へ、同時に又は経時的
に、それぞれ異なる種類の培地又は同一種類の培地を、
隔壁の高さよりも高く且つ各区画内から溢れ出ないよう
に注入することを特徴とする。Further, according to the method for producing a fractionation medium of the present invention, a fractionation container having a partition therein and divided into a plurality of compartments is prepared, and the compartments of the fractionation container are simultaneously or continuously stored in each compartment. In each case, different types of media or the same type of media,
It is characterized in that the injection is performed so as to be higher than the height of the partition wall and not to overflow from the inside of each section.
【０００９】本発明において分画培地とは、内部に隔壁
を有し複数の区画に分画されている分画容器内に培地が
注入されている状態のものをいう。本発明において容器
とは、シャーレ、培養ビン、培養管等の微生物を培養す
るための容器をいう。In the present invention, the term "fractionation medium" refers to a state in which a culture medium is injected into a fractionation container having a partition therein and divided into a plurality of compartments. In the present invention, the container refers to a container for culturing microorganisms such as a petri dish, a culture bottle, a culture tube, and the like.
【００１０】[0010]
【作用】本発明の分画培地によれば、分画容器内の培地
表面の高さが、隔壁の高さよりも高いので、微生物をエ
ーゼ等の接種手段により接種するために、隔壁をまたが
って各区画内の培地へ接種手段を移動させる場合におい
て、接種手段の先端の押圧により培地が多少窪んでも、
剛性の隔壁に対して接種手段の先端の引っ掛かりが少な
く、その移動がスムーズに行える。また、一つの分画容
器内の分画された各区画内へ培地を注入する際には、そ
の上部空間の任意の位置から注入することができ、しか
も各区画内へ同時に注入することができ、各区画内の培
地に混入する気泡は上部の開放面から容易に排出され
る。According to the fractionation medium of the present invention, the height of the surface of the culture medium in the fractionation vessel is higher than the height of the partition walls. When moving the inoculation means to the culture medium in each section, even if the culture medium is slightly depressed by pressing the tip of the inoculation means,
The distal end of the inoculation means is less likely to be caught on the rigid partition wall and can be moved smoothly. In addition, when injecting the culture medium into each of the fractionated compartments in one fractionation container, the culture medium can be injected from any position in the upper space, and can be simultaneously injected into each compartment. Air bubbles mixed into the culture medium in each compartment are easily discharged from the upper open surface.
【００１１】本発明の分画培地によれば、分画容器内の
隔壁近傍の底部、側壁近傍の底部及び／又は隔壁部には
培地の剥離防止手段が設けられているので、隔壁の高さ
が低くても（従って、培地と隔壁との接触面積が少なく
ても）この剥離防止手段により培地をしっかりと隔壁に
固定することができ、培地が収縮したとしても培地の隔
壁からの剥離が防止される。According to the fractionation medium of the present invention, the bottom near the partition, the bottom near the side wall and / or the partition in the fractionation vessel are provided with a means for preventing the separation of the culture, so that the height of the partition is high. Even if the medium is low (thus, even if the contact area between the culture medium and the partition wall is small), the separation preventing means can firmly fix the culture medium to the partition wall and prevent the culture medium from separating from the partition wall even if the culture medium shrinks. Is done.
【００１２】[0012]
【実施例】本発明の構成を実施例として示した図面に基
づき具体的に説明する。図１は本発明の分画培地の一実
施例としての培地入分画シャーレ本体の斜視図であり、
図２はその上面図、図３はその断面図である。１はシャ
ーレ本体、２はシャーレ内に突設され、シャーレ内の空
間を分画して隔てる隔壁、３は隔壁２によって分画され
たシャーレ内の一つの空間に注入された培地Ａ、４はシ
ャーレ内の培地Ａとは別の分画された空間に注入された
培地Ｂ、５はシャーレ本体１の側壁である。このシャー
レ本体１に図示していないシャーレ蓋体が嵌合されて本
実施例の培地入分画シャーレを構成する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The construction of the present invention will be specifically described with reference to the drawings showing the embodiments. FIG. 1 is a perspective view of a medium-filled petri dish main body as one embodiment of the fractionation medium of the present invention,
FIG. 2 is a top view thereof, and FIG. 3 is a sectional view thereof. 1 is a petri dish main body, 2 is protrudingly provided in the petri dish, and a partition for separating and separating the space in the petri dish, 3 is a medium A injected into one space in the petri dish divided by the partition 2, and 4 The culture media B and 5 injected into the fractionated space different from the culture media A in the petri dish are the side walls of the petri dish main body 1. A Petri dish lid (not shown) is fitted to the Petri dish main body 1 to constitute a Petri dish with a medium in the present embodiment.
【００１３】本実施例の培地入分画シャーレの隔壁２の
高さは、側壁５の高さよりも低く、且つ注入された培地
３，４表面よりも低くなっている。したがって、培地を
シャーレに注入するにあたって、培地の量は、隔壁２の
高さよりも盛り上がるように培地を注入することが重要
である。このように、培地３，４の表面の高さを調整す
ることによって、微生物をエーゼ等の接種手段により接
種する際に、隔壁２をまたがって各培地３，４へ接種手
段を移動させても、接種手段の先端が隔壁２へ引っ掛か
かることが軽減され、その移動がスムーズとなる。The height of the partition walls 2 of the medium-containing fractionation dish of the present embodiment is lower than the height of the side wall 5 and lower than the surfaces of the injected culture media 3 and 4. Therefore, when injecting the culture medium into the petri dish, it is important to inject the culture medium so that the amount of the culture medium rises higher than the height of the partition wall 2. In this way, by adjusting the surface height of the culture media 3 and 4, when the microorganisms are inoculated by the inoculation means such as Ese, the inoculation means can be moved to the respective culture media 3 and 4 across the partition wall 2. In addition, the tip of the inoculation means is less likely to be caught on the partition wall 2, and the movement is smooth.
【００１４】シャーレ本体１の材質はガラス又はプラス
チックのいずれも使用できる。分画シャーレの全体形状
は、円、正方形、等の任意の形状とすることができる。
分画シャーレの各区画は、２個、３個、４個、それ以上
の区画に分けることができる。図４は３分画されている
円状シャーレ、図５は４分画されている円状シャーレ、
図６は２分画されている正方形シャーレ、図７は４分画
されている正方形シャーレの一例である。各区画を分け
る隔壁の先端の形状は、図８にその断面を示すとおり角
付であってもよいし、図９にその断面を示すとおりＲ付
であってもよいが、エーゼ等の使用時においては、角付
の方がややスムーズ感があった。また本実施例の培地入
分画シャーレは、手作業の微生物の接種だけではなく、
機械的な接種手段であるスパイラルシステムに適用した
場合にもスムーズに行なえた。The material of the petri dish body 1 can be either glass or plastic. The whole shape of the fractionation petri dish can be any shape such as a circle, a square, and the like.
Each section of the fractionation dish can be divided into two, three, four, and more sections. FIG. 4 shows a circular petri dish divided into three parts, FIG. 5 shows a circular petri dish divided into four parts,
FIG. 6 shows an example of a square petri dish divided into two parts, and FIG. 7 shows an example of a square petri dish divided into four parts. The shape of the tip of the partition wall that divides each section may be square as shown in the cross section in FIG. 8 or may be R as shown in the cross section in FIG. In the above, the cornering was slightly smoother. In addition, the Petri dish with medium in the present example is not only a manual inoculation of microorganisms,
Even when applied to a spiral system which is a mechanical inoculation means, it could be performed smoothly.
【００１５】本実施例における培地入分画シャーレの隔
壁２の高さは、前記のとおり培地３，４表面よりも低い
ため、培地３，４と隔壁２との接触面積が少なくなる傾
向にある。したがって、培地３，４と隔壁２との剥離を
防止するために、シャーレ内の底部及び／又は隔壁部に
剥離防止手段を設けることが望ましい。図１０は隔壁２
付近の断面図であり、隔壁２の周辺に設けた剥離防止手
段６を表している。図１１は本実施例における剥離防止
手段６が形成された培地入分画シャーレの一例の上面図
を示し、図１２は同じく図１１とは別の例の剥離防止手
段６が形成された培地入分画シャーレの上面図である。Since the height of the partition walls 2 of the medium-containing fractionation dish in this embodiment is lower than the surfaces of the culture media 3 and 4 as described above, the contact area between the culture media 3 and 4 and the partition walls 2 tends to decrease. . Therefore, in order to prevent the separation between the culture media 3 and 4 and the partition 2, it is desirable to provide a separation preventing means at the bottom and / or the partition in the petri dish. FIG.
FIG. 2 is a cross-sectional view of the vicinity, showing a peeling prevention unit 6 provided around a partition 2. FIG. 11 shows a top view of an example of a medium-containing separation petri dish in which the peeling preventing means 6 according to the present embodiment is formed, and FIG. It is a top view of a fractionation petri dish.
【００１６】図１０、図１１及び図１２の剥離防止手段
６は、隔壁２から一定の間隔を保って平行に設けられ、
シャーレ底面から凸状に***した帯状の突起物から形成
されている。この突起物は、複数本設けられてもよい。
この突起物の高さは隔壁２の高さよりも低く、好ましく
は隔壁２の高さよりも１／９〜１／３程度がよく、その
形状は椀状のものがよい。図１２の剥離防止手段６は、
隔壁２の近傍及び分画シャーレの側壁５から一定の間隔
を保って平行に設けられ、シャーレ底面から凸状に***
した帯状の突起物から形成されている。The separation preventing means 6 shown in FIGS. 10, 11 and 12 is provided in parallel with a certain distance from the partition 2, and
It is formed from a strip-like projection protruding from the bottom of the Petri dish. A plurality of the protrusions may be provided.
The height of the projection is lower than the height of the partition 2, preferably about 1/9 to 1/3 of the height of the partition 2, and the shape thereof is preferably a bowl shape. The peel prevention means 6 in FIG.
It is provided in parallel with a fixed distance from the vicinity of the partition wall 2 and from the side wall 5 of the separating petri dish, and is formed of a band-like projection protruding from the bottom of the petri dish in a convex shape.
【００１７】図１３は剥離防止手段６である扇状の突起
物が複数個、隔壁２の壁面に設けられた分画シャーレの
隔壁近傍の斜視図であり、図１４はその断面図であり、
図１５はその上面図である。また図１６は一個の扇状の
突起物が隔壁２の壁面の一側に設けられた分画シャーレ
の隔壁近傍の斜視図であり、図１７はその断面図であ
り、図１８はその上面図である。このように、剥離防止
手段６を分画シャーレ内に設けることにより、培地が隔
壁２から剥離することを防止し、剥がれ強度を強くして
いる。FIG. 13 is a perspective view showing the vicinity of a partition of a separating petri dish provided with a plurality of fan-shaped projections as the peeling preventing means 6 provided on the wall surface of the partition 2, and FIG.
FIG. 15 is a top view thereof. 16 is a perspective view of the vicinity of the partition wall of the separating petri dish in which one fan-shaped projection is provided on one side of the wall surface of the partition wall 2, FIG. 17 is a sectional view thereof, and FIG. 18 is a top view thereof. is there. As described above, by providing the separation preventing means 6 in the separating petri dish, the culture medium is prevented from being separated from the partition wall 2 and the separation strength is increased.
【００１８】上記実施例は微生物を培養する容器に関し
シャーレについて説明したが、本発明が適用される容器
はシャーレに限定されず、培養ビン、培養管等の微生物
を培養するためのどのような容器でも可能である。Although the above embodiment has been described with respect to a dish for culturing microorganisms, the vessel to which the present invention is applied is not limited to a petri dish, and any vessel for culturing microorganisms, such as a culture bottle or a culture tube. But it is possible.
【００１９】[0019]
【発明の効果】本発明の分画培地は、分画容器本体の上
部開放面から培地の注入がなされるものであり、各分画
室への培地の注入を同時に行なうことが可能であり、培
地の表面には平坦な封止体が必要ではなく、したがっ
て、微生物の接種時には該封止体を剥離する作業がな
く、特に、機械的に大量の容器に微生物を接種する場合
に有利であり、微生物学的な検査、実験が省力化でき
る。According to the fractionation medium of the present invention, the culture medium is injected from the upper open surface of the fractionation container main body, and it is possible to simultaneously inject the culture medium into each compartment. There is no need for a flat sealing body on the surface, and therefore, there is no work of peeling off the sealing body at the time of inoculating microorganisms, which is advantageous particularly when mechanically inoculating microorganisms into a large amount of containers, Microbiological examinations and experiments can be saved.
【００２０】本発明の分画培地は、培地表面が隔壁より
も盛り上がっているために、微生物の接種時にエーゼ等
の接種手段の引っ掛かりが少なく、その接種手段の移動
をスムーズに行なうことができる。In the fractionation medium of the present invention, the surface of the medium is raised above the partition walls, so that the inoculation means such as ose is less likely to be caught at the time of inoculation of microorganisms, and the inoculation means can be moved smoothly.
【００２１】本発明の分画培地において、培地の各分画
室への注入時にたとえ培地内に気泡が混入したとして
も、注入面は開放された空間であるので、気泡は容易に
放出でき、培地内の気泡の残留を防げることができる。In the fractionation medium of the present invention, even if bubbles are mixed in the medium when the medium is injected into each compartment, the injection surface is an open space, so that the bubbles can be easily released, and It is possible to prevent air bubbles from remaining inside.
【図１】本発明の一実施例のシャーレ本体の斜視図であ
る。FIG. 1 is a perspective view of a petri dish main body according to an embodiment of the present invention.
【図２】本発明の一実施例のシャーレ本体の上面図であ
る。FIG. 2 is a top view of a petri dish main body according to one embodiment of the present invention.
【図３】本発明の一実施例のシャーレ本体の断面図であ
る。FIG. 3 is a sectional view of a petri dish main body according to one embodiment of the present invention.
【図４】実施例の３分画されている円状シャーレの一例
である。FIG. 4 is an example of a circular petri dish divided into three parts according to the embodiment.
【図５】実施例の４分画されている円状シャーレの一例
である。FIG. 5 is an example of a four-part circular petri dish of the embodiment.
【図６】実施例の２分画されている正方形シャーレの一
例である。FIG. 6 is an example of a square petri dish divided into two pieces according to the embodiment.
【図７】実施例の４分画されている正方形シャーレの一
例である。FIG. 7 is an example of a square petri dish divided into four parts according to the embodiment.
【図８】実施例の培地入分画シャーレの各区画を分ける
隔壁の先端の形状を示し、その先端が角付のものを示
す。FIG. 8 shows the shape of the tip of a partition which divides each section of the medium-filled petri dish of the example, and shows the tip with a corner.
【図９】実施例の培地入分画シャーレの各区画を分ける
隔壁の先端の形状を示し、その先端がＲ付のものを示
す。FIG. 9 shows the shape of the tip of a partition for dividing each section of the medium-fractionated petri dish of the example, and shows the tip with an R.
【図１０】実施例の培地入分画シャーレの隔壁付近の断
面図であり、隔壁の周辺に設けた剥離防止手段を表して
いる。FIG. 10 is a cross-sectional view showing the vicinity of a partition wall of the medium-containing fractionation petri dish of the example, showing a peeling preventing means provided around the partition wall.
【図１１】実施例の培地入分画シャーレの剥離防止手段
が形成された培地入分画シャーレの一例の上面図であ
る。FIG. 11 is a top view of an example of a medium-filled petri dish in which the means for preventing separation of the medium-filled petri dish of the example is formed.
【図１２】図１１とは別の例の培地入分画シャーレの剥
離防止手段が形成された培地入分画シャーレの上面図で
ある。FIG. 12 is a top view of another example of the medium-filled petri dish in which the means for preventing separation of the medium-filled Petri dish is different from that of FIG.
【図１３】扇状の突起物が複数個、隔壁の壁面に設けら
れた実施例の分画シャーレの隔壁近傍の斜視図である。FIG. 13 is a perspective view of the vicinity of the partition wall of the dividing petri dish of the embodiment in which a plurality of fan-shaped protrusions are provided on the wall surface of the partition wall.
【図１４】扇状の突起物が複数個、隔壁の壁面に設けら
れた実施例の分画シャーレの隔壁近傍の断面図である。FIG. 14 is a cross-sectional view showing the vicinity of the partition wall of the fractionation dish of the embodiment in which a plurality of fan-shaped protrusions are provided on the wall surface of the partition wall.
【図１５】扇状の突起物が複数個、隔壁の壁面に設けら
れた実施例の分画シャーレの隔壁近傍の上面図である。FIG. 15 is a top view showing the vicinity of the partition wall of the separating petri dish of the embodiment in which a plurality of fan-shaped protrusions are provided on the wall surface of the partition wall.
【図１６】扇状の突起物が一個、隔壁の壁面に設けられ
た実施例の分画シャーレの隔壁近傍の斜視図である。FIG. 16 is a perspective view of the vicinity of the partition wall of the fractionation dish of the embodiment in which one fan-shaped protrusion is provided on the wall surface of the partition wall.
【図１７】扇状の突起物が一個、隔壁の壁面に設けられ
た実施例の分画シャーレの隔壁近傍の断面図である。FIG. 17 is a cross-sectional view showing the vicinity of the partition wall of the fractionation petri dish of the embodiment in which one fan-shaped protrusion is provided on the wall surface of the partition wall.
【図１８】扇状の突起物が一個、隔壁の壁面に設けられ
た実施例の分画シャーレの隔壁近傍の上面図である。FIG. 18 is a top view of the vicinity of the partition wall of the fractionation dish of the embodiment in which one fan-shaped protrusion is provided on the wall surface of the partition wall.
１ シャーレ本体 ２ 隔壁 ３ 培地Ａ ４ 培地Ｂ ５ 側壁 ６ 剥離防止手段 DESCRIPTION OF SYMBOLS 1 Petri dish main body 2 Partition wall 3 Medium A 4 Medium B 5 Side wall 6 Peeling prevention means
───────────────────────────────────────────────────── フロントページの続き (72)発明者 仙石 博 東京都豊島区巣鴨２丁目11番１号 日水 製薬株式会社内 (56)参考文献 特開 昭62−232396（ＪＰ，Ａ) 実開 昭62−1598（ＪＰ，Ｕ) 実開 平５−82299（ＪＰ，Ｕ) 実開 昭59−44298（ＪＰ，Ｕ) ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Hiroshi Sengoku 2-11-1, Sugamo, Toshima-ku, Tokyo Nissui Pharmaceutical Co., Ltd. (56) References JP-A-62-232396 (JP, A) 62-1598 (JP, U) Japanese Utility Model Hei 5-82299 (JP, U) Japanese Utility Model Application Showa 59-44298 (JP, U)
Claims (4)
ている分画容器の各区画内に培地が注入されている分画
培地において、該培地表面の高さが、前記隔壁の高さよ
りも高いことを特徴とする分画培地。1. A fractionation medium in which a culture medium is injected into each compartment of a fractionation container having a partition therein and divided into a plurality of compartments, wherein the height of the surface of the culture medium is equal to the height of the partition wall. A fractionation medium characterized by being higher than the height.
剥離を防止するために、分画容器内には剥離防止手段が
設けられていることを特徴とする請求項１記載の分画培
地。2. The separation container according to claim 1, wherein a separation preventing means is provided in the separation container in order to prevent separation between a wall surface of the separation container and the culture medium. Fractionation medium.
近傍の底部、側壁近傍の底部及び／又は隔壁部に設けら
れた突起物であることを特徴とする請求項２記載の分画
培地。3. The separation according to claim 2, wherein the separation preventing means is a protrusion provided on a bottom near the partition, a bottom near the side wall and / or the partition in the separation container. Culture medium.
画されている分画容器を用意し、 （２）前記分画容器の各区画内へ、同時に又は経時的
に、それぞれ異なる種類の培地又は同一種類の培地を、
隔壁の高さよりも高く且つ各区画内から溢れ出ないよう
に注入することを特徴とする分画培地の製造方法。4. A fractionation container having a partition wall therein and fractionated into a plurality of compartments is prepared, and (2) into each compartment of the fractionation container simultaneously or with time. Different types of media or the same type of media,
A method for producing a fractionation medium, wherein the injection is performed so as to be higher than the height of the partition wall so as not to overflow from each section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6121928A JP2627142B2 (en) | 1994-05-11 | 1994-05-11 | Fractionation medium and method for producing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6121928A JP2627142B2 (en) | 1994-05-11 | 1994-05-11 | Fractionation medium and method for producing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07303470A JPH07303470A (en) | 1995-11-21 |
| JP2627142B2 true JP2627142B2 (en) | 1997-07-02 |
Family
ID=14823398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6121928A Expired - Lifetime JP2627142B2 (en) | 1994-05-11 | 1994-05-11 | Fractionation medium and method for producing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2627142B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4547067B2 (en) * | 2000-05-02 | 2010-09-22 | 株式会社日研生物医学研究所 | Microbial fractionation medium and petri dish for production of the fractionation medium |
| KR100679248B1 (en) * | 2005-04-27 | 2007-02-05 | 서울시립대학교 산학협력단 | Culture dish with comparted-cells using same medium |
| EP2148921B1 (en) | 2007-05-30 | 2017-07-19 | Nikon Corporation | Incubation container |
| JP5682121B2 (en) * | 2010-03-04 | 2015-03-11 | 大日本印刷株式会社 | Petri dish for preparation of fractionation medium |
| JP6171681B2 (en) * | 2013-07-29 | 2017-08-02 | 大日本印刷株式会社 | Microbe culture sheet |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3902972A (en) * | 1973-02-02 | 1975-09-02 | Corning Glass Works | Modified petri dish for automatic inoculation |
| JPS574879Y2 (en) * | 1977-01-10 | 1982-01-29 | ||
| JPS5551200U (en) * | 1978-10-02 | 1980-04-04 | ||
| JPS5944298U (en) * | 1982-09-17 | 1984-03-23 | 藤田 信一 | Shear tray for bacterial testing |
| JPS63137671A (en) * | 1986-11-28 | 1988-06-09 | Erumetsukusu:Kk | Petri dish for holding medium |
-
1994
- 1994-05-11 JP JP6121928A patent/JP2627142B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07303470A (en) | 1995-11-21 |
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