KR20050104970A - Method for producing ramie leaves and barley leaves flour and foods including the flour - Google Patents
Method for producing ramie leaves and barley leaves flour and foods including the flour Download PDFInfo
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- KR20050104970A KR20050104970A KR1020040030444A KR20040030444A KR20050104970A KR 20050104970 A KR20050104970 A KR 20050104970A KR 1020040030444 A KR1020040030444 A KR 1020040030444A KR 20040030444 A KR20040030444 A KR 20040030444A KR 20050104970 A KR20050104970 A KR 20050104970A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/51—Concentration
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Abstract
본 발명은 모시잎과 보리잎 분말의 제조방법 및 그 분말을 포함하는 기능성 식품에 관한 것으로, 더욱 상세하게는 모시잎과 월동한 어린 보리잎에 용매를 가하여 일정온도에서 추출액을 추출하고, 이를 감압농축, 동결건조 및 분쇄하여 분말로 제조한 후, 상기 분말을 이용하여 빵, 국수, 냉면, 빙과류, 드링크류, 차, 약용주, 과립제 등의 식품에 첨가하거나 화장비누, 미용팩 등을 제조함으로써, 플라노이드, 루틴, 엽록소 등의 필수영양소를 손쉽게 섭취할 수 있고, 빈혈, 변비개선, 항염작용, 피부미용 등에 우수한 효과를 제공하도록 한 것으로, 모시잎과 월동한 어린 보리잎을 채취하여 증류수로 세척하는 단계(S1)와; 세척한 모시잎과 보리잎을 72∼96시간 동안 음건하는 단계(S2)와; 음건한 모시잎과 보리잎 10∼20중량%에 용매 80∼90중량%를 가하고 65∼100℃에서 4∼6시간 추출하는 단계(S3)와; 추출된 추출액을 45∼55℃에서 감압농축하는 단계(S4)와; 감압농축된 농축액을 -10∼0℃에서 동결건조하는 단계(S5)와; 동결건조된 건조물을 15∼400mesh의 입도로 분쇄하는 단계(S6)를 포함하여 제조되는 것을 특징으로 한다.The present invention relates to a method for producing ramie leaves and barley leaf powder, and to a functional food comprising the powder. More specifically, the extract is extracted at a constant temperature by adding a solvent to the ramie leaves and wintering young barley leaves, Concentrate, lyophilize and pulverize to prepare powders, and then use the powders to add to foods such as bread, noodles, cold noodles, frozen fruits, drinks, tea, medicinal liquor, granules, or make cosmetic soaps or cosmetic packs. Essential nutrients such as nooids, rutin, and chlorophyll can be easily ingested and provided to provide an excellent effect on anemia, constipation improvement, anti-inflammatory action, skin care, etc. Step S1; Drying the washed ramie leaves and barley leaves for 72 to 96 hours (S2); Adding 80-90 wt% of solvent to 10-20 wt% of dry ramie and barley leaves and extracting at 65-100 ° C. for 4-6 hours (S3); Concentrating the extracted extract under reduced pressure at 45 ~ 55 ℃ (S4) and; Lyophilizing the concentrated solution under reduced pressure at -10 to 0 ° C (S5); The freeze-dried dry material is characterized in that it comprises a step (S6) to grind to a particle size of 15 ~ 400mesh.
Description
본 발명은 모시잎과 보리잎 분말의 제조방법 및 그 분말을 포함하는 기능성 식품에 관한 것으로, 더욱 상세하게는 모시잎과 월동한 어린 보리잎에 용매를 가하여 일정온도에서 추출액을 추출하고, 이를 감압농축, 동결건조 및 분쇄하여 분말로 제조한 후, 상기 분말을 이용하여 빵, 국수, 냉면, 빙과류, 드링크류, 차, 약용주, 과립제 등의 식품에 첨가하거나 화장비누, 미용팩 등을 제조함으로써, 플라노이드, 루틴, 엽록소 등의 필수영양소를 손쉽게 섭취할 수 있고, 빈혈, 변비개선, 항염작용, 피부미용 등에 우수한 효과를 제공하며, 모시잎과 보리잎 식품의 식품적 가치를 향상시킬 뿐만 아니라 저렴한 가격의 모시잎과 보리잎을 이용하여 고부가가치를 창출할 수 있도록 한 것이다. The present invention relates to a method for producing ramie leaves and barley leaf powder, and to a functional food comprising the powder. More specifically, the extract is extracted at a constant temperature by adding a solvent to the ramie leaves and wintering young barley leaves, Concentrate, lyophilize and pulverize to prepare powders, and then use the powders to add to foods such as bread, noodles, cold noodles, frozen fruits, drinks, tea, medicinal liquor, granules, or make cosmetic soaps or cosmetic packs. Easily ingest essential nutrients such as nooids, rutin, and chlorophyll, and provide excellent effects on anemia, constipation improvement, anti-inflammatory action, skin care, etc. It is designed to create high added value by using ramie leaves and barley leaves.
우리가 살고 있는 생활환경은, 많은 공해 속에 공기, 매연, 소음, 오래된 식수, 잔류 농약, 육체적인 피로, 정신적인 갈등 속에 있으며, 이러한 환경에서 생활을 하면 인체 내의 활성산소가 증가하여 인체에 해로운 작용을 일으켜 발병의 원인이 되는 것으로 알려져 있다.The living environment we live in is a lot of pollution in the air, soot, noise, old drinking water, residual pesticides, physical fatigue, and mental conflict. It is known to cause the onset.
이와 같은 현상으로 인해 얻은 병은, 생활 습관이 원인이 되고 있는 것이고 이러한 원인 제공을 감소 또는 제거하기 위하여, 항산화제 또는 항산화작용을 하는 식품을 섭취하도록 장려하고 있는 실정이다.The disease resulting from such a phenomenon is caused by a lifestyle, and in order to reduce or eliminate the cause provision, it is encouraging to take an antioxidant or food with an antioxidant action.
이러한 유해한 활성산소를 제거하려면 적당한 운동을 하고 맑은 공기, 청정음료수를 흡수, 섭취하며 생활환경을 개선하면 어느 정도 제거에 도움이 되지만, 더욱 중요시 되는 것이 식품으로서 항산화 성분을 섭취하는 것이다.To remove these harmful free radicals, exercise appropriately, absorb and consume clean air, clean drinking water, and improve the living environment to some extent, but more importantly, take antioxidants as food.
이러한 항산화 성분이 포함된 식품은 천연재로서 모시잎, 보리잎 등을 찾을 수 있는 데, 모시잎은 다년생 풀로서 남부지방에서 옷감용으로 재배되고 있으며, 활성산소 제거와 항산화작용이 있는 플라보노이드와 폴리페놀류를 함유하고 있어 생체에 유익한 기능성 식품으로의 개발이 요구되고 있으며, 보리잎 역시 항산화작용이 있는 비타민 C, 비타민 E와 음식물 대사에 절대적으로 필요로 되는 비타민 B1, B2, B6 가 많이 함유되어 있으며, 인체에 필요한 광물질로서, 칼륨, 나트륨, 마그네슘, 칼슘, 철, 구리 등을 풍부하게 함유하고 있어 변비해소, 노폐물 배설, 해독, 간경화, 황달에 유효하며, 피부각질 연화에도 도움이 있어, 식품, 미용제품 등으로의 활용이 요구되고 있다.Foods containing these antioxidants can be found as raw materials such as ramie leaves and barley leaves. The ramie leaves are a perennial grass grown in the southern provinces for cloth, and are free from flavonoids and polys with free radicals and antioxidant activity. As it contains phenols, it is required to be developed as a functional food that is beneficial to the living body, and barley leaves also contain vitamin C, vitamin E, and vitamin B 1 , B 2 , and B 6 which are absolutely necessary for metabolism of food. It is rich in potassium, sodium, magnesium, calcium, iron, and copper as a mineral necessary for the human body. It is effective for eliminating constipation, waste excretion, detoxification, cirrhosis and jaundice, and also helps soften dead skin cells. It is required to be used as food, beauty products and the like.
이러한 모시잎과 보리잎을 실생활에 이용하기 위하여 모시잎은 5월∼6월경에 어린 잎을 절취하여 잘 세척한 후 분쇄하고, 보리잎은 겨울을 넘긴 어린 보리잎을 3월부터 4월 초순까지 채취하여 잘 세척한 후 음건 및 분쇄하여 이용하여 왔으나, 모시잎이나 보리잎을 직접 분쇄하여 분말화하여 식품이나 비누 등에 이용하면 식품의 식감이 좋지 못하고, 비누의 질감 또한 저하되어 사용이 불편하였을 뿐만 아니라 거품이 잘 나지 않는 등의 문제점이 있었다.In order to use these ramie leaves and barley leaves in real life, the ramie leaves are cut and washed well after May to June, and the barley leaves are crushed young barley leaves over the winter from March to early April. After harvesting and washing well, it has been used in the shade and crushed, but when mashing the ramie leaves or barley leaves directly to powder and use them in food or soap, the texture of the food is not good, and the texture of the soap is also degraded, resulting in inconvenience. But there was a problem such as poor foaming.
이러한 문제점을 해결하기 위하여 국내특허공보 제2002-73354호의 '보리등겨가루를 이용한 기능성 발효식품'은 보리등겨가루를 발효시켜 메주를 제조하고, 제조된 메주로서 발효 보리등겨가루 성형품(환, 과립)을 제조하였으나, 보리등겨가루를 발효시킴으로써 식감이 저하되고 보리등겨가루의 활용도가 제한되는 등의 문제점이 있었으며, 또 다른 국내특허공보 제1997-5123호의 '보리 어린잎 분말과 즙을 이용한 과립의 제조방법'은 단순히 보리어린잎을 미세하게 분쇄하여 이용하였을 뿐 식품의 식감이나 비누의 촉감이 저하되는 것을 방지하지 못한 문제점이 있었다.In order to solve this problem, 'Functional fermented food using barley bran powder' of Korean Patent Publication No. 2002-73354 is prepared by fermenting barley bran powder to make meju, and fermented barley bran powder molded product (round, granule) However, by fermenting barley bran powder, there is a problem that the texture is lowered and the utilization of barley bran powder is limited. Another Korean Patent Publication No. 1997-5123 discloses the preparation of granules using barley young leaf powder and juice. Method 'simply used to finely grind the barley leaves, there was a problem that can not prevent the texture of food or the touch of soap is lowered.
따라서 상기와 같은 종래의 문제점을 해결하기 위한 본 발명의 목적은, 모시잎과 월동한 어린 보리잎에 용매를 가하여 일정온도에서 추출액을 추출하고, 이를 감압농축, 동결건조 및 분쇄하여 분말로 제조한 후, 상기 분말을 이용하여 빵, 국수, 냉면, 빙과류, 드링크류, 차, 약용주, 과립제 등의 식품에 첨가하거나 화장비누, 미용팩 등을 제조함으로써, 플라노이드, 루틴, 엽록소 등의 필수영양소를 손쉽게 섭취할 수 있고, 빈혈, 변비개선, 항염작용, 피부미용 등에 우수한 효과를 제공하며, 모시잎과 보리잎 식품의 식품적 가치를 향상시킬 수 있도록 하는 모시잎과 보리잎 분말의 제조방법 및 그 분말을 포함하는 기능성 식품을 제공함에 있다.Therefore, an object of the present invention for solving the conventional problems as described above, by adding a solvent to the ramie leaves and wintering young barley leaves to extract the extract at a constant temperature, it was concentrated under reduced pressure, lyophilized and ground to prepare a powder Afterwards, the powder is added to foods such as bread, noodles, cold noodles, ice cream, drinks, tea, medicinal liquor, and granules, or by preparing cosmetic soaps, cosmetic packs, etc., essential nutrients such as flanoids, rutin, chlorophyll, etc. Method for preparing ramie leaf and barley leaf powder which can be ingested, provide excellent effects on anemia, constipation improvement, anti-inflammatory action, skin care, and improve the food value of ramie leaf and barley leaf food In providing a functional food comprising a.
상기한 목적을 달성하기 위한 본 발명의 모시잎과 보리잎 분말의 제조방법 및 그 분말을 포함하는 기능성 식품에 의하면, 모시잎과 월동한 어린 보리잎을 채취하여 증류수로 세척하는 단계(S1)와; 세척한 모시잎과 보리잎을 72∼96시간 동안 음건하는 단계(S2)와; 음건한 모시잎과 보리잎 10∼20중량%에 용매 80∼90중량%를 가하고 65∼100℃에서 4∼6시간 추출하는 단계(S3)와; 추출된 추출액을 45∼55℃에서 감압농축하는 단계(S4)와; 감압농축된 농축액을 -10∼0℃에서 동결건조하는 단계(S5)와; 동결건조된 건조물을 15∼400mesh의 입도로 분쇄하는 단계(S6)를 포함하여 제조되는 것을 특징으로 한다.According to the present invention for achieving the above object and the method of producing a powder and barley leaf powder of the present invention and a functional food comprising the powder, step of collecting the young barley and winter barley leaves and washed with distilled water (S1) and ; Drying the washed ramie leaves and barley leaves for 72 to 96 hours (S2); Adding 80-90 wt% of solvent to 10-20 wt% of dry ramie and barley leaves and extracting at 65-100 ° C. for 4-6 hours (S3); Concentrating the extracted extract under reduced pressure at 45 ~ 55 ℃ (S4) and; Lyophilizing the concentrated solution under reduced pressure at -10 to 0 ° C (S5); The freeze-dried dry material is characterized in that it comprises a step (S6) to grind to a particle size of 15 ~ 400mesh.
이하 본 발명이 속하는 기술분야에서 통상의 지식을 가진자가 본 발명을 용이하게 실시할 수 있을 정도로 본 발명의 바람직한 실시예를 첨부된 도면을 참조하여 상세하게 설명하면 다음과 같다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings such that those skilled in the art can easily implement the present invention.
도 1은 본 발명에 의한 모시잎과 보리잎 분말의 제조공정도로서, 세척(S1), 음건(S2), 추출(S3), 감압농축(S4), 동결건조(S5), 분쇄단계(S6)로 된다.1 is a manufacturing process diagram of the ramie leaf and barley leaf powder according to the present invention, washing (S1), shade (S2), extraction (S3), reduced pressure concentration (S4), freeze drying (S5), grinding step (S6) do.
먼저, 모시잎과 월동한 어린 보리잎을 채취하여 증류수로 3∼4회 세척(S1)하여 이물질을 완전히 제거한 후, 세척한 모시잎과 어린 보리잎을 72∼96시간 동안 음건(S2)하여 수분을 제거하도록 한다. First, the young barley leaves and wintering young barley leaves are collected and washed three to four times with distilled water (S1) to completely remove foreign matters, and then the dried ramie leaves and young barley leaves are dried in the shade (S2) for 72 to 96 hours. Remove it.
상기 음건시간이 72시간 미만이면 모시잎과 보리잎에 함유된 수분이 충분히 제거되지 못하며 96시간을 초과하면 생산성이 좋지 못하므로 72∼96시간 동안 음건(S2)한다.When the shade time is less than 72 hours, the moisture contained in the ramie leaves and barley leaves are not sufficiently removed, and when the shade time exceeds 96 hours, the productivity is not good, so it is shaded for 72 to 96 hours (S2).
음건한 모시잎과 보리잎에 추출용매를 가하고 추출액을 추출(S3)하되, 음건한 모시잎과 보리잎의 배합비가 10중량% 미만이면 추출액의 농도가 낮아 생산성이 좋지 못하며, 20중량%를 초과하면 용매가 적어 충분히 추출되지 못하므로 모시잎과 보리잎 10∼20중량%에 용매 80∼90중량%를 가하고 65∼100℃에서 4∼6시간 추출(S3)한다. Extract solvent to the dry ramie leaves and barley leaves and extract the extract (S3), but if the blending ratio of the dry ramie leaves and barley leaves is less than 10% by weight, the concentration of the extract is low, the productivity is not good, and exceeds 20% by weight When the solvent is small, it cannot be extracted sufficiently, so that 80 to 90% by weight of the solvent is added to 10 to 20% by weight of the ramie and barley leaves and extracted for 4 to 6 hours at 65 to 100 ° C (S3).
이 때 상기 모시잎과 보리잎은 요구되는 목적에 맞게 혼합하여 사용하는 바, 그 혼합비가 1:1 중량비가 될 수도 있는 것이고 2:1이 될 수도 있는 것으로, 모시잎에 포함된 유효성분이 더 필요로 되는 식품이나 미용제품을 제조할 경우는 모시잎을 보리잎보다 더 많이 첨가하여 엑기스를 추출하고, 보리잎이 더 많이 필요로 되는 경우에는 보리잎을 더 많이 첨가하여 추출하도록 한다.At this time, the ramie leaves and barley leaves are used to mix according to the desired purpose, the mixing ratio may be 1: 1 weight ratio may be 2: 1, more active ingredients contained in the ramie leaves When manufacturing a food or beauty products to be added more than the barley leaves to extract the extract, if more barley leaves are required to add more barley leaves to extract.
또한 추출온도가 65℃ 미만이면 추출이 어려우며 100℃를 초과하면 열에 의해 유효성분이 손실될 수 있고, 추출시간이 4시간 미만이면 유효성분이 충분히 추출되지 못하고 6시간을 초과하여도 더 이상의 유효성분이 추출되지 않으므로, 65∼100℃에서 4∼6시간 추출하도록 한다.In addition, if the extraction temperature is less than 65 ℃ extraction is difficult, if it exceeds 100 ℃ the active ingredient may be lost by heat, if the extraction time is less than 4 hours, the active ingredient is not extracted enough, even if more than 6 hours no more active ingredient is extracted Therefore, it extracts at 65-100 degreeC for 4 to 6 hours.
이 때 상기 추출용매로는 일반적으로 쉽게 사용할 수 있는 증류수를 이용할 수 있음은 물론이며, 물과 알코올이 혼합된 혼합액을 가하여 추출하면 모시잎과 보리잎 내에 포함된 수용성 성분과 지용성 성분 모두를 용출할 수 있는 것이다. In this case, as the extraction solvent, it is generally possible to use distilled water which can be easily used. Extracting by adding a mixed solution of water and alcohol will elute both the water-soluble and fat-soluble components contained in the ramie and barley leaves. It can be.
상기 알코올과 물의 배합비는 85 : 10∼20 중량비로 하여 모시잎과 보리잎 내의 유효성분이 충분히 추출될 수 있도록 한다. 이 때 상기 혼합용매의 배합비가 85: 10 부피비보다 물이 적게 투입되면 수용성 성분의 추출이 어렵고, 85: 20 중량비를 초과하면 지용성 성분의 추출이 수용성 성분의 추출에 비에 적게 되므로, 그 최적의 비를 선택하면 85 : 10∼20 중량비가 가장 양호한 것이다.The mixing ratio of the alcohol and water is 85:10 to 20 by weight so that the active ingredient in the ramie leaves and barley leaves can be sufficiently extracted. At this time, if the mixing ratio of the mixed solvent is less water than 85: 10 by volume ratio, the extraction of water-soluble components is difficult, and when the 85: 20 weight ratio is exceeded, the extraction of fat-soluble components is less than the ratio of extraction of water-soluble components, the optimum If the ratio is selected, the 85:10 to 20 weight ratio is the best.
상기 추출된 추출액을 45∼55℃에서 감압농축(S4)하여 농축액을 제조한다.The extracted extract is concentrated under reduced pressure (S4) at 45 ~ 55 ℃ to prepare a concentrate.
이 때 농축온도가 45℃ 미만이면 농축시 시간이 많이 소요되어 생산성이 좋지 못하고 55℃를 초과하면 유효성분이 파괴될 우려가 있으므로, 45∼55℃의 온도범위에서 서서히 감압하여 농축하도록 한다.At this time, if the concentration temperature is less than 45 ℃ concentration takes a lot of time when the concentration is not good, if it exceeds 55 ℃ there is a risk that the active ingredient is destroyed, so to be concentrated under reduced pressure slowly in the temperature range of 45 ~ 55 ℃.
감압농축된 농축액을 -10∼0℃에서 동결건조(S5)하여 모시잎과 보리잎이 특성이 그대로 유지되면서도 복수성(復水性)이 뛰어난 건조물을 얻는다. 상기 동결온도가 -10℃ 미만이면 에너지 소모량이 많아 경제적이지 못하고, 0℃를 초과하면 동결이 이루어지지 않으므로 -10∼0℃로 하는 것이 바람직하다.The concentrated solution concentrated under reduced pressure was lyophilized (S5) at -10 to 0 ° C. to obtain a dried product having excellent plurality, while the ramie leaves and the barley leaves retained their properties. If the freezing temperature is less than -10 ° C much energy consumption is not economical, and if the freezing temperature exceeds 0 ° C it is preferable that the freezing temperature is -10 to 0 ° C.
동결건조(S5) 후, 동결건조된 건조물을 분쇄(S6)하여 기능성 식품이나 비누, 팩 등의 제품에 쉽게 사용할 수 있도록 하고, 건조물의 입도를 15∼400mesh로 하여 쉽게 분산되지 않으면서 이용이 편리하도록 한다.After lyophilization (S5), the lyophilized dry matter is pulverized (S6) so that it can be easily used in functional foods, soaps, packs, and other products. Do it.
상기 건조물의 입도가 15mesh 보다 크면 입도가 커 식품, 비누, 팩 등의 제품에 첨가하면 제품의 식감 또는 질감을 저하시킬 우려가 있으며, 400mesh 보다 작으면 입도가 작아 쉽게 비산되므로 사용이 불편하므로, 상기 건조물을 15∼400mesh의 입도로 분쇄한다. If the particle size of the dried product is larger than 15 mesh, the particle size may be increased, so that the texture or texture of the product may be reduced when added to a product such as food, soap, or pack. The dried product is ground to a particle size of 15 to 400 mesh.
상기와 같은 방법으로 엑기스화하여 제조된 모시잎과 보리잎 분말은 직접분쇄한 것과는 달리 식품으로 사용할 시에는 식감이 우수하고, 비누 등의 제품에 사용할 시에도 비누의 성질이 저하되지 않으면서도 보리잎과 모시잎의 성분을 함유하도록 하여 현대인의 건강과 기호에 맞는 고품질의 제품을 생산할 수 있는 것이다.Unlike the direct grinding, the ramie leaf and barley leaf powder prepared by extracting in the same manner as described above have excellent texture when used as food, and even when used in products such as barley, the barley leaf does not deteriorate even when the soap properties are reduced. It is made to contain the ingredients of fruit and ramie leaves to produce high quality products that meet the health and taste of modern people.
즉 상기와 같이 제조된 모시잎과 보리잎 분말을 유효성분으로 포함시켜 빵, 국수, 냉면, 빙과류, 드링크류, 차, 약용주, 과립제 등의 식품에 첨가하거나 화장비누, 미용팩 등을 제조하면 식감이 우수하면서도 인체에 필요한 필수영양분을 쉽게 섭취할 수 있고 간편한 세안, 팩 만으로 피부미용 효과를 나타낼 수 있게 되는 것이다.That is, by including the ramie leaf and barley leaf powder prepared as described above as an active ingredient, it is added to foods such as bread, noodles, cold noodles, ice cream, drinks, tea, medicinal liquor, granules, etc. Excellent yet easy to consume the essential nutrients needed by the human body and easy to wash, packs will be able to show the skin beauty effect.
예를 들면 상기와 같이 제조한 모시잎과 보리잎분말과 기타의 생약재를 고두밥과 곡차로 발효하여 약용주(藥用酒)를 제조하면 약용주의 혈액순환 개선과 소화촉진 작용으로 식욕증진, 피로회복, 체력보강에 많은 도움을 줄 수 있을 뿐만 아니라 항산화 작용과 간기능 개선 및 해독에 유효한 효과를 발휘하는 것이다.For example, fermenting ramie leaves, barley leaf powder, and other herbal medicines prepared as described above with gourd rice and grain tea to produce medicinal liquor (藥用 酒), which improves blood circulation and promotes digestion, increases appetite, recovers fatigue, Not only can it help to strengthen stamina, but it is also effective in antioxidant activity and liver function improvement and detoxification.
본 발명에 사용된 모시잎과 어린 보리잎에 대해 살펴보면 다음과 같은 특징을 갖는다.Looking at the ramie leaves and young barley leaves used in the present invention has the following characteristics.
서천지방의 특산품인 모시잎은 플라보노이드(flavonoid), 루틴(lutin), 폴리페놀류(polyphenol), 엽록소, 비타민C, 글루타민 등이 함유되어 있어 활성산소 제거, 항산화작용, 혈관확대 작용으로 인한 혈압강하는 물론 순환기 계에 유익하게 작용하고 피생성을 도와, 지혈, 어혈제거, 각혈, 토혈, 항문부종치유, 타박상, 외상출혈, 급성유선염, 탈항증, 소염, 뱀과 벌레 물린데 치료, 월경과다 등에 유용한 효과를 갖는다. Moshi leaf, a special product of Seocheon, contains flavonoids, lutins, polyphenols, chlorophyll, vitamin C, and glutamine. Of course, it is beneficial to the circulatory system and helps to produce, useful for hemostasis, bleeding, hemorrhage, hemostasis, anal edema, bruises, traumatic bleeding, acute mastitis, anti-inflammation, anti-inflammatory, snake and insect bites Has
월동한 어린 보리잎은 나트륨, 칼륨, 마그네슘, 철, 구리 등의 광물질을 풍부하게 함유하고 있음과 더불어, 각종 비타민, 엽록소 등을 포함하고 있어, 황달치료, 이뇨, 손발이 트고 아픈데, 소염작용, 항암작용, 소화작용, 갈증해, 부종치료, 항산화작용, 변비개선, 피부색소침착억제작용, 당뇨 등의 효과를 갖는다.Overwintering young barley leaves contain abundant minerals such as sodium, potassium, magnesium, iron, and copper, and contain various vitamins and chlorophyll. Anti-cancer, digestive action, thirst, edema treatment, antioxidant action, constipation improvement, skin pigmentation inhibition effect, diabetes has the effect.
이하 실시예를 통하여 본 발명을 더욱 상세히 설명하도록 한다.Hereinafter, the present invention will be described in more detail with reference to the following examples.
(실시예 1)(Example 1)
모시잎과 어린 보리잎을 채취하여 증류수로 3회 세척하고, 세척한 모시잎과 보리잎을 80시간 동안 음건하였다. 상기 음건한 모시잎과 보리잎 각각 100g에 증류수 900ml를 가하고 70℃에서 4시간 추출 후, 추출한 추출액을 50℃에서 감압농축하고 -5℃에서 동결건조하였으며, 이를 다시 200mesh로 분쇄하여 모시잎과 보리잎의 엑기스를 제조하였다.Ramie leaves and young barley leaves were collected and washed three times with distilled water, and the washed ramie leaves and barley leaves were dried for 80 hours. After distilled water 900ml was added to 100g of each of the dried ramie leaves and barley leaves and extracted for 4 hours at 70 ° C, the extracted extract was concentrated under reduced pressure at 50 ° C and lyophilized at -5 ° C. An extract of the leaves was prepared.
(비교예 1)(Comparative Example 1)
상기 실시예 1과 같은 방법으로 모시잎만을 엑기스화하여 제조하였다.It was prepared by extracting only the ramie leaves in the same manner as in Example 1.
(비교예 2)(Comparative Example 2)
상기 실시예 1과 같은 방법으로 라미(lamie)를 엑기스화하여 제조하였다.In the same manner as in Example 1 was prepared by extracting a lami (lamie).
상기와 같이 제조한 각 실시예 및 비교예의 항산화 효과를 비교하기 위하여, 하기와 같은 방법으로 실험을 실시하였다.In order to compare the antioxidant effect of each Example and Comparative Example prepared as described above, the experiment was carried out as follows.
하기 표 1은 본 발명의 실시예 및 비교예들의 항산화 효과의 실험결과이다.Table 1 below is an experimental result of the antioxidant effect of the examples and comparative examples of the present invention.
상기 DPPH free radical 소거작용은 안정한 free radical 인 DPPH(1,1-diphenyl- 2-picrylhydrazyl)을 이용한 Blois의 방법에 준하였다. 검액 추출물을 메탄올로 실험에 필요로 하는 농도로 희석하여 각 농도당 4ml 씩 시험관에 옮기고, 여기에 1×10-4M DPPH의 메탄올 용액 1ml을 가한 다음 잘 혼합한 후 실온에서 30분간 방치하고 520nm에서 흡광도를 측정하여 DPPH를 환원시키는 정도로부터 항산화 활성을 구하였다.The DPPH free radical scavenging action was based on Blois' method using DPPH (1,1-diphenyl-2-picrylhydrazyl) as a stable free radical. Dilute the sample extract with methanol to the concentration required for the experiment, and transfer it to the test tube for 4 ml of each concentration, add 1 ml of methanol solution of 1 × 10-4 M DPPH, mix well, and leave it for 30 minutes at room temperature. The absorbance was measured and antioxidant activity was calculated | required from the grade which reduces DPPH.
Xanthine Oxidase 저해활성은 NBT(Nitro blue tetrazolium)법에 준하였다. 즉, 0.05M sodium carbonate buffer 0.48ml에 3mM xanthine, 3mM EDTA, 0.15% BSA 및 0.75mM NBT를 각각 0.02ml 씩 가하여 잘 혼합하였다. 여기에 증류수에 필요로 한 농도로 희석한 검액 0.02ml 씩 각각 가한 후 실온에서 10분간 방치한 다음 xanthine oxidase 6mU를 넣어 25℃에서 20분간 가온하여 반응시키고 6mM CuCl2 0.02ml를 넣어 반응을 종결시킨 뒤 560nm에서 흡광도를 측정하여 비교관찰하였다.Xanthine Oxidase inhibitory activity was determined according to NBT (Nitro blue tetrazolium) method. In other words, 0.08 ml of 3 mM xanthine, 3 mM EDTA, 0.15% BSA and 0.75 mM NBT were added to 0.48 ml of 0.05 M sodium carbonate buffer. Add 0.02ml of each sample diluted to the required concentration in distilled water, and leave it at room temperature for 10 minutes, add 6mU of xanthine oxidase, and react at 20 ℃ for 20 minutes, and add 0.02ml of 6mM CuCl2 to terminate the reaction. Absorbance was measured at 560 nm and compared.
적혈구막 안정화 작용은, Miki 등의 방법을 약간 변형하여 적혈구막 안정화작용을 측정하였다. 즉, heparin을 처리한 주사기로 흰쥐로부터 심장채혈하여 원심분리(1,500rpm, 10분)로 혈장을 분리한 다음 10배의 0.15M NaCl을 가하여 다시 원심분리하여 상등액을 제거하여 적혈구를 분리하며 이 조작을 3회 반복하여 적혈구를 분리하였다. 분리한 적혈구를 PBS(10mM sodium phosphate buffer, 152mM Nacl)로 10배 희석하여 실험에 이용하였다. 희석된 적혈구 0.2ml를 취하여 37℃ 5분간 pre-incubation 한 뒤 PBS에 녹인 검액 0.2ml 씩 각각 가하고, 이어서 100mM AAPH[2,2'-azobis (2-amidinopropane) dihydrochloride] 0.2ml를 가하여 잘 혼합한 후 37℃에서 3시간동안 반응시킨다. 각 반응용액에 20배의 0.15M NaCl 용액과 증류수를 각각 가하여 혼합한 다음 원심분리(1000xg, 10분)한 후 상등액을 540nm에서 각각의 흡광도를 측정하여 용혈도 및 적혈구 용혈억제활성을 다음 식으로부터 산출하였다.The erythroid membrane stabilization effect was measured by slightly modifying the method of Miki et al. In other words, heparin-treated syringes were used to collect blood from rats, and then plasma was separated by centrifugation (1,500 rpm, 10 minutes). Then, 10 times 0.15 M NaCl was added and centrifuged again to remove supernatant to separate red blood cells. Was repeated three times to separate red blood cells. The isolated red blood cells were diluted 10-fold with PBS (10 mM sodium phosphate buffer, 152 mM Nacl) and used for the experiment. Take 0.2 ml of diluted red blood cells, pre-incubate for 5 minutes at 37 ° C, and add 0.2 ml of each sample solution dissolved in PBS, then add 0.2 ml of 100 mM AAPH [2,2'-azobis (2-amidinopropane) dihydrochloride] and mix well. After reaction at 37 ℃ for 3 hours. Each reaction solution was mixed with 20 times 0.15M NaCl solution and distilled water, and then centrifuged (1000xg, 10 minutes). The supernatant was measured for absorbance at 540 nm, and hemolysis and erythrocyte hemolytic activity were determined from the following equation. Calculated.
용혈도(%) = A/B×100Hemolysis Degree (%) = A / B × 100
A : 0.15M NaCl 용액을 가한 검체의 흡광도 A: Absorbance of the sample to which 0.15M NaCl solution was added
B : 증류수를 가한 검체의 흡광도 B: Absorbance of Specimens Added Distilled Water
적혈구 용혈 억제활성(%) = [(Hcontrol - Hsample)/(Hcontrol - Hblank )] ×100Erythrocyte hemolytic inhibitory activity (%) = [(H control -H sample ) / (H control -H blank )] × 100
Hcontrol : 시료를 첨가하지 않은 대조군의 용혈도H control : Hemolysis degree of control group without sample
Hsample : 시료를 첨가한 실험군의 용혈도H sample : Hemolysis degree of test group
Hblank : 시료 및 AAPH를 첨가하지 않은 Blank의 용혈도H blank : Hemolysis degree of blank without sample and AAPH
간 균질화물의 지질과산화 형성에 미치는 작용은, 흰쥐를 ether로 마취시키고 복부를 절개하여 간문맥에 polyethylene tube를 삽입하여 4℃ 0.9% NaCl 용액으로 간을 세척한 후 적출하여 균질화하였다. 간 균질화물을 0.9% NaCl 용액으로 세척하고 다시 간(습중량)과 1.15% KCl용액의 비가 1:9의 비율로 1.15% KCl용액을 가하여 glass homogenizer로 다시 균질화하였다. 지질과산화형성 억제효과는 T. Yokozawa 등의 방법을 이용하여 thiobarbituric acid (TBA)-reactive substance 법에 따라 실시하였다. 즉 phosphate 완충액(50mM, pH 7.4) 0.8ml와 30mM H2O2 0.3ml를 각각 시험관에 옮기고 간 균질화물 0.5ml를 가하여 혼합하였다. 뚜껑을 막고 37℃에서 20분간 incubation시키고 반응정지액으로 TBA/thiochloric acid(TCA)/HCl(5% TBA, 15% TCA, 0.25N HCl) 4.0ml를 가하여 반응을 정지시켰다. 냉수 중에서 10분간 빙냉시키고 10분간 3,000rpm에서 10분간 원심 분리하여 상청액을 535nm에서 흡광도를 측정하여 지질과산화물 형성억제효과를 산출하였다. 표준물질로서 tetramethoxy propan(1∼10 nmol)을 상기와 동일한 방법으로 흡광도를 측정하여 과산화지질량, 즉 malondialdehyde(MDA)량(nmol/mg protein)으로서 표시하였다.The effect of hepatic homogenate on lipid peroxidation was anesthetized with ether, incised in the abdomen, polyethylene tube was inserted into the portal vein, the liver was washed with 4 ° C 0.9% NaCl solution, and extracted and homogenized. The liver homogenate was washed with 0.9% NaCl solution, and again homogenized with a glass homogenizer by adding 1.15% KCl solution in a ratio of 1: 9 of liver (wet weight) and 1.15% KCl solution. Lipid peroxidation inhibitory effect was performed according to thiobarbituric acid (TBA) -reactive substance method using T. Yokozawa et al. That is, 0.8 ml of phosphate buffer (50 mM, pH 7.4) and 0.3 ml of 30 mM H 2 O 2 were transferred to a test tube, and 0.5 ml of liver homogenate was added and mixed. The lid was closed and incubated at 37 ° C. for 20 minutes. The reaction was stopped by adding 4.0 ml of TBA / thiochloric acid (TCA) / HCl (5% TBA, 15% TCA, 0.25N HCl) as the reaction stopper. Ice cooling was performed in cold water for 10 minutes and centrifugation at 3,000 rpm for 10 minutes for 10 minutes to measure the absorbance at 535 nm to calculate lipid peroxide formation inhibitory effect. As a standard, tetramethoxy propan (1-10 nmol) was measured for absorbance in the same manner as described above, and expressed as the amount of peroxide, namely malondialdehyde (MDA) (nmol / mg protein).
상기 표 1에서 알 수 있는 바와 같이 DPPH scavenging 효과는 각 실시예 및 비교예의 IC50은 각각 0.502mg/mL, 0.665mg/mL 및 0.713mg/mL 이었으며, xanthine oxidase 저해효과는 실시예 1과 비교예1은 각각 0.74mg/mL과 0.77mg/mL이었고, 비교예 2는 별다른 영향을 미치지 못하였다. 그리고, 적혈구막의 보호작용을 지표로 한 실험에서 실시예, 비교예1, 비교예2의 IC50는 각각 0.47mg/mL, 0.45mg/mL, 2.77mg/mL 이었다. 또한 지질과산화 형성 억제를 지표로 한 실험에서는 각 검체의 IC50은 55.6mg/mL , 69.2mg/mL 및 73.5mg/mL로서, 실시예와 비교예1은 강한 항산화활성이 인정되었으며, 그 중 특히 실시예가 더욱 우수한 항산화활성이 인정되었다.As can be seen in Table 1, the DPPH scavenging effect was 0.502 mg / mL, 0.665 mg / mL, and 0.713 mg / mL, respectively, and the IC 50 of each example and the comparative example were xanthine oxidase inhibitory effect of Example 1 and Comparative Example. 1 was 0.74 mg / mL and 0.77 mg / mL, respectively, and Comparative Example 2 had no effect. In the experiments using the protective action of erythrocytes, the IC 50 of Examples, Comparative Examples 1 and 2 was 0.47 mg / mL, 0.45 mg / mL, and 2.77 mg / mL, respectively. In the experiments with the inhibition of lipid peroxidation formation, the IC 50 of each sample was 55.6 mg / mL, 69.2 mg / mL, and 73.5 mg / mL. Examples and Comparative Example 1 showed strong antioxidant activity. The example was recognized for its superior antioxidant activity.
상기한 실시예에서 알 수 있는 바와 같이 본 발명의 모시잎과 보리잎 분말은 현대 사회의 복잡한 환경에서 오는 갈등으로 인해 쌓인 정신적, 육체적인 피로를 쉽게 해소시킬 수 있는 것이다.As can be seen in the above embodiment, the ramie leaf and barley leaf powder of the present invention can easily solve the mental and physical fatigue accumulated due to the conflict in the complex environment of modern society.
또한 본 발명의 방법은 모시잎과 보리잎의 혼합시에만 적용되는 것이 아닌 식용가능한 생약제인 산수유, 두충, 구기자, 황정을 일정한 비율로 혼합하여 상기와 같은 제조공정을 통해 제조하여 약용주, 기능성 과립제, 정제, 드링크제 등에 제조하는 것도 가능한 것은 물론이며, 본 발명의 분말에 별도의 비타민 B1, B2, B 6, B12, 비타민 D3, 비타민 C, 비타민 E 광물질로서의 칼슘, 마그네슘, 아연, 철, 칼륨 등을 추가하여 식품을 제조할 수 있는 것이다.In addition, the method of the present invention is not applied only when the mixing of ramie leaves and barley leaves mixed with edible medicinal herbs such as cornus, tofu, goji berry, yellow cherries in a constant ratio manufactured by the manufacturing process as described above medicinal liquor, functional granules, purified, and it is of course possible for manufacturing such drinks, the powder of the present invention separate vitamin B 1, B 2, B 6, B 12, vitamin D 3, vitamin C, calcium as vitamin E minerals, magnesium, zinc, iron Food can be prepared by adding potassium and the like.
이상에서와 같이 본 발명을 비록 상기의 실시예에 한하여 설명하였지만 반드시 여기에만 한정되는 것은 아니며, 식품, 비누용품 뿐만 아니라 비타민류, 아미노산류, 미네랄류 등에 배합하여 사용할 수 도 있는 것으로, 본 발명의 범주와 사상을 벗어나지 않는 범위 내에서 다양한 변형실시가 가능함은 물론이다.Although the present invention as described above has been described only in the above embodiments, it is not necessarily limited thereto, and may be used in combination with vitamins, amino acids, minerals, etc. as well as food and soap products, and the scope of the present invention. Various modifications can be made without departing from the spirit and scope of course.
이상의 설명에서 분명히 알 수 있듯이 본 발명의 모시잎과 보리잎 분말의 제조방법 및 그 분말을 포함하는 기능성 식품에 의하면, 모시잎과 월동한 어린 보리잎에 용매를 가하여 일정온도에서 추출액을 추출하고, 이를 감압농축, 동결건조 및 분쇄하여 분말로 제조한 후, 상기 분말을 이용하여 빵, 국수, 냉면, 빙과류, 드링크류, 차, 약용주, 과립제 등의 식품에 첨가하거나 화장비누, 미용팩 등을 제조함으로써, 플라노이드, 루틴, 엽록소 등의 필수영양소를 손쉽게 섭취할 수 있고, 빈혈, 변비개선, 항염작용, 피부미용 등에 우수한 효과를 제공하며, 모시잎과 보리잎 식품의 식품적 가치를 향상시킬 뿐만 아니라 저렴한 가격의 모시잎과 보리잎을 이용하여 고부가가치를 창출할 수 있도록 하는 등의 유용한 효과를 제공한다.As apparent from the above description, according to the present invention, a method of preparing the ramie leaf and barley leaf powder and a functional food including the powder, extract the extract at a predetermined temperature by adding a solvent to the ramie leaf and the overwintered young barley leaf, It is concentrated under reduced pressure, lyophilized and pulverized to prepare a powder, and then the powder is added to foods such as bread, noodles, cold noodles, frozen desserts, drinks, tea, medicinal liquor, granules, or by making cosmetic soaps or cosmetic packs. Easily ingest essential nutrients such as, flanoid, rutin, chlorophyll, and provide excellent effects on anemia, constipation improvement, anti-inflammatory action, skin care, and not only improve the food value of ramie leaf and barley leaf foods. It provides useful effects such as the creation of high value using low priced ramie leaves and barley leaves.
또한 물과 알콜의 혼합용매를 사용하여 추출함으로써, 수용성 유효성분과 지용성 유효성분이 충분히 추출될 수 있도록 하고, 본 발명의 분말을 이용하여 약용주 등과 같은 기능성식품과 미용제품을 제조함으로써, 빈혈, 변비개선, 간장기능강화, 황달치유, 해독작용, 피부미용 등에 우수한 효과를 제공한다.In addition, by using a mixed solvent of water and alcohol to extract the water-soluble active ingredient and fat-soluble active ingredient is sufficiently extracted, and by using the powder of the present invention by preparing a functional food and beauty products such as medicinal liquor, anemia, constipation improvement, It provides an excellent effect on liver function enhancement, jaundice healing, detoxification and skin care.
도 1은 본 발명에 의한 모시잎과 보리잎 분말의 제조공정도.1 is a manufacturing process of the ramie leaf and barley leaf powder according to the present invention.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101125801B1 (en) * | 2009-10-19 | 2012-03-27 | 양성규 | The ramie and the Chinese matrimony vine, Salviae miltiorrhizae the health beverage manufacturing method which contains and the healthy beverage |
| KR101233133B1 (en) * | 2011-04-04 | 2013-02-14 | 이영옥 | Seasoned laver with ramie and manufacturing method thereof |
| KR101256811B1 (en) * | 2010-03-23 | 2013-04-22 | 한국식품연구원 | Preparation method of rice cookie using rice powder treated enzyme |
| KR101332567B1 (en) * | 2012-04-24 | 2013-11-25 | 김영득 | Manufacturing method of walnut cake comprising ramie leaves flour |
| KR101387157B1 (en) * | 2012-09-10 | 2014-04-21 | 최종만 | Meat broth making method including mature process with grain powder contained high calcium made of safflower or ramie fabric leaf or pickled egg and dough manufacturing using that |
| KR101404146B1 (en) * | 2012-12-03 | 2014-06-05 | 김선경 | Method for preparing ramie tea powder |
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| KR100858593B1 (en) | 2008-01-02 | 2008-09-17 | 김종임 | Cold noodle composition including ramie and manufacture method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101125801B1 (en) * | 2009-10-19 | 2012-03-27 | 양성규 | The ramie and the Chinese matrimony vine, Salviae miltiorrhizae the health beverage manufacturing method which contains and the healthy beverage |
| KR101256811B1 (en) * | 2010-03-23 | 2013-04-22 | 한국식품연구원 | Preparation method of rice cookie using rice powder treated enzyme |
| US10137159B2 (en) * | 2010-12-27 | 2018-11-27 | Industrial Technology Research Institute | Boehmeria extract and its use in treating liver diseases |
| KR101233133B1 (en) * | 2011-04-04 | 2013-02-14 | 이영옥 | Seasoned laver with ramie and manufacturing method thereof |
| KR101332567B1 (en) * | 2012-04-24 | 2013-11-25 | 김영득 | Manufacturing method of walnut cake comprising ramie leaves flour |
| KR101387157B1 (en) * | 2012-09-10 | 2014-04-21 | 최종만 | Meat broth making method including mature process with grain powder contained high calcium made of safflower or ramie fabric leaf or pickled egg and dough manufacturing using that |
| KR101404146B1 (en) * | 2012-12-03 | 2014-06-05 | 김선경 | Method for preparing ramie tea powder |
| KR20150069175A (en) * | 2013-12-13 | 2015-06-23 | 원광대학교산학협력단 | A fermented boehmeria nivea for protecting brain neuroral cells and a fermented tea using the fermented boehmeria nivea |
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