KR20030017300A - Cosmetic compositions containing extracts of Prunus armeniaca L, Vitis vinifera L. and Perilla frutescens var. japonica Hara - Google Patents
Cosmetic compositions containing extracts of Prunus armeniaca L, Vitis vinifera L. and Perilla frutescens var. japonica Hara Download PDFInfo
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
Description
본 발명은 살구씨, 포도씨, 들깨 혼합 추출물을 함유하는 화장료 조성물로서, 보다 상세하게는 살구씨, 포도씨, 들깨로부터 추출한 비독성 유기산 및 불포화 지방산을 포함하여 각질 제거 효과, 세포증식효과 및 항산화 효과가 있는 화장료 조성물에 관한 것이다.The present invention is a cosmetic composition containing apricot seed, grape seed, perilla mixed extract, more specifically, including a non-toxic organic acid and unsaturated fatty acid extracted from apricot seed, grape seed, perilla, exfoliation effect, cell proliferation effect and antioxidant effect It relates to a cosmetic composition.
각질층은 표피중의 기저세포, 유극세포, 과립세포의 형태적 특징이 순차적으로 변화하면서 피부 표면층으로 이동하는 세포로서, 최종적으로는 각질세포가 된다. 이런 표피세포의 분화과정을 각화라고 하는데, 각질세포는 차례차례로 계속해서 만들어지며, 결국 최 외곽 층의 오래된 '때'로서 이탈해 가기 때문에 표피는 일정한 두께를 유지한다. 이렇게 통상적으로 새로운 세포층으로 바뀌어 가는 것은 턴 오버(Turn over)라고 하며, 부위나 나이에 따라 다르지만, 정상적인 피부에서는 이 표피의 턴 오버는 대략 4 주일이라고 말하고 있다. 외부의 이물질의 침입이나 각질층의 파괴 등이 일어나면, 표피세포는 민감하게 반응하여 기저세포의 분열이 왕성하게 되고, 턴 오버 속도도 빨라져서 이물질의 제거나 각질층이 복원되게 한다. 또 화학적 자극이나 물리적 자극이 반복해서 피부에 가해지면 각질층이 두꺼워진다. 이러한 반응은 어떤 외부의 자극에 대한 표피의 방어반응이라고 간주할 수 있다. 그러나 각질층이 이러한 방어반응으로 인해 지나치게 두꺼워지면, 외관적으로 각질이 갈라지는 동시에 꺼칠꺼칠해지며, 피문(皮汶)이 불명확해지고 극도의 경우에는 피문(皮汶)이 완전히 소실되어 버리는 경우도 있다.The stratum corneum is a cell that migrates to the skin surface layer while the morphological characteristics of the basal cells, the pole cells, and the granule cells in the epidermis change sequentially, and finally become the keratinocytes. This differentiation process of epidermal cells is called keratinization. The keratinocytes are made in sequence and eventually break away as the old 'time' of the outermost layer. This transition to a new cell layer is commonly called turn over, which depends on the site and age, but in normal skin, the epidermis's turnover is about four weeks. When external foreign matters invade or destroy the stratum corneum, the epidermal cells react sensitively and the basal cells divide actively, and the turnover speed is increased to remove the foreign matter or restore the stratum corneum. In addition, repeated chemical and physical stimuli to the skin thicken the stratum corneum. This response can be thought of as a protective response of the epidermis to any external stimulus. However, if the stratum corneum becomes excessively thick due to this protective reaction, the stratum corneum splits at the same time, and the rust is apparent, and in extreme cases, the rust is completely lost.
이러한 이유 때문에 과다하게 축적된 각질을 제거하면서 동시에 세포 증식 능력, 항산화 기능을 강화시켜야 하며, 항상 부드럽고, 노화되지 않으며, 건강한 피부를 유지하기 위한 올바른 피부 관리를 해주는 것이 중요하다.For this reason, it is important to remove excess dead skin cells and at the same time to enhance cell proliferation and antioxidant function, and to maintain proper skin care to keep skin soft, aging and healthy at all times.
본 발명에서는 피부에 과다하게 축적되어 있는 각질을 제거하고, 간혹 각질 제거에 의해 생길 수 있는 피부의 손상을 막고, 동시에 세포를 증식시켜 건강한 피부를 유지하기 위한 천연 추출물을 포함하는 각질 제거용 화장료 조성물을 제공하는 것이다.In the present invention, the exfoliation cosmetic composition comprising a natural extract for removing the dead skin accumulated in the skin, preventing the damage of the skin that may be caused by the exfoliation, and at the same time to proliferate the cells to maintain healthy skin To provide.
도 1은 본 발명의 화장료 조성물의 항산화 효과를 나타내는 그래프.1 is a graph showing the antioxidant effect of the cosmetic composition of the present invention.
도 2는 본 발명의 화장료 조성물의 세포보호효과를 나타내는 그래프.Figure 2 is a graph showing the cytoprotective effect of the cosmetic composition of the present invention.
도 3은 본 발명의 화장료 조성물의 세포증식효과를 나타내는 그래프.Figure 3 is a graph showing the cell proliferation effect of the cosmetic composition of the present invention.
도 4는 본 발명의 화장료 조성물의 보습 효과를 나타내는 그래프.Figure 4 is a graph showing the moisturizing effect of the cosmetic composition of the present invention.
도 5는 본 발명의 화장료 조성물의 피부개선효과를 나타내는 디지털 이미지 분석 결과.5 is a digital image analysis result showing the skin improvement effect of the cosmetic composition of the present invention.
상기의 본 발명의 목적은 살구씨, 포도씨, 들깨로부터 분리·정제한 천연 유기산 및 불포화 지방산을 함유하는 각질 제거용 화장료 조성물에 의해 달성된다.The above object of the present invention is achieved by the exfoliation cosmetic composition containing natural organic acid and unsaturated fatty acid separated and purified from apricot seed, grape seed, perilla.
본 발명의 발명자들은 피부에 지나치게 축적된 각질을 제거함과 동시에 상처 치유효과, 세포 증식, 그리고 항산화 효과를 갖는 물질을 찾고자하여 자연의 여러 가지 천연물을 검색한 결과, 살구씨, 포도씨, 들깨로부터 추출, 정제한 유기산과 불포화 지방산이 매우 우수한 각질 제거 효과와 상처 치유효과, 세포 증식효과, 항산화 효과를 나타냄을 확인 할 수 있었다. 또한 한약제로 사용되는 원료로서 안전성 문제를 동시에 해결하였다.The inventors of the present invention search for various natural products to remove substances that have accumulated excessively on the skin and at the same time have a wound healing effect, cell proliferation, and antioxidant effect, and extracted from apricot seed, grape seed, perilla, Purified organic acid and unsaturated fatty acid showed very excellent exfoliation effect, wound healing effect, cell proliferation effect and antioxidant effect. In addition, as a raw material used as a herbal medicine solved the safety problem at the same time.
원료에 대하여 알아보면, 살구씨(Prunus armeniaca L.)는 장미과 식물에 속하며, <본초경집주> 에 나온다. 기침, 가래, 천식을 그치게 하고, 기름기가 많아 장을 원활하게 해주어 통변이 잘되게 한다. 주성분으로 아미그달린 약 3%, 지방유 약 50%, 단백질 및 각종 유리산, Citric acid, Malic acid 등이며, 그 외에 칼륨과 인이 특히 많이 들어있고, 당질, 칼슘, 나트륨, 섬유질, 비타민 A, B, C등을 함유하고 있다.In terms of raw materials, apricot seeds (Prunus armeniaca L.) belong to the rose family, which is found in the Herb Gardener. Stop coughing, sputum, asthma, and greasy, smooth bowel, so that you have a good bowel movement. Its main components are about 3% of amidaline, about 50% of fat oil, protein and various free acids, citric acid and malic acid, etc., and are particularly rich in potassium and phosphorus, and include sugars, calcium, sodium, fiber, vitamins A, B, It contains C.
포도씨(Vitis vinifera L.)는 <동의보감>, <도경본초>, <신농본초경>, <체험실록> 등에서 포도는 배고픔을 달래고, 기운이 나게 하며 추위를 타지 않게 하고, 이뇨 작용이 있어 오줌을 잘 나오게 한다. 기혈과 근골을 보강하고 비위와 폐신을 보하여 몸이 든든하게 하며 태아를 편안하게 하고 포도씨앗을 암 예방에도 효과가있다고 전해진다. 특히 최근에는 항산화작용, 피로회복, 골다공증, 성인병 예방에 탁월한 효과가 있다고 보고된 바 있다. 성인병에 좋은 리놀렌산, β-씨토스테롤, α-토코페롤 등이 많이 함유되어 있어 동맥경화증, 고혈압등의 성인병, 지질과산화 억제를 하는 항산화 역할을 한다. 주석산과 살신산, 탄닌, 안토시아닌 등으로 몸의 독소를 제거하며 항암효과와 각종 성인병에 탁월한 효과가 있다.Grape seeds (Vitis vinifera L.) in <agreement agreement>, <dougyeongboncho>, <new agricultural herbs>, <experience memorandum>, etc. grapes soothe hunger, cheer up, do not burn cold, there is a diuretic action urine well To come out. It is said to be effective in reinforcing the blood and musculature, making the body healthy by watching the stomach and lungs, making the fetus comfortable, and the grape seed also effective in preventing cancer. In particular, it has recently been reported that there is an excellent effect on antioxidant activity, fatigue recovery, osteoporosis, prevention of adult diseases. Linolenic acid, β-cytosterol, and α-tocopherol, which are good for adult diseases, contain a lot of adult diseases such as arteriosclerosis, hypertension, and antioxidants that inhibit lipid peroxidation. It removes toxins from the body with tartaric acid, salicylic acid, tannin, and anthocyanin, and has excellent anti-cancer effects and various adult diseases.
들깨(Perilla frutescens var. japonica Hara) 는 꿀풀과에 속하며 <본초도경>, <음편신참>에 나오며, 임자, 백소자라고도 한다. 희미한 향기가 있고 깨물면 기름이 진하게 느껴진다. 효능은 하기, 소담, 윤폐, 창자를 편하게 한다. 체했거나 위, 복부의 통증, 임신복통 등에 효과가 있고, 주요성분으로는 리놀렌산, 팔미틴산 등이 있다.Perilla frutescens var. Japonica Hara belongs to the Lamiaceae family and appears in Herbs and Herbs. It has a faint scent and the oil feels thick when bitten. Efficacy in relieving tingling, diarrhea, rot and bowel. Pregnancy or stomach, abdominal pain, pregnancy abdominal pain, etc. The main ingredients are linolenic acid, palmitic acid and the like.
상기한 바와 같이, 살구씨, 포도씨, 들깨 혼합 추출물은 각질제거에 효능이 있는 유기산과 염증 등에 효능이 있는 불포화 지방산 그리고 각종 미네랄 등이 다량 함유되어 있는 것을 알 수 있다. 이와 같은 효능 물질을 통하여 살구씨, 포도씨, 들깨 혼합 추출물을 이용한 각질제거 및 주름 방지용 화장료에 적용이 가능하다.As described above, it can be seen that the apricot seed, grape seed, and perilla mixed extract contains a large amount of organic acids, unsaturated fatty acids, and various minerals, which are effective in exfoliation, and the like. It is possible to apply to cosmetics for exfoliation and anti-wrinkle using apricot seed, grape seed, perilla mixed extract through such an efficacy substance.
본 발명의 화장료 조성물을 일정농도 이상 배합하여 사람 피부에 직접 도포한 실험 결과, 거칠고 건조해 있던 피부가 일정시간 지난 후 촉촉하고 보드라운 피부로 바뀐 것을 알 수 있었다. 이 사실은 피부의 각질제거 효과와 세포증식효과, 상처치유효과, 항산화 효과를 가지고 있다는 것을 재차 확인한 결과라 할 수 있다.As a result of experiments in which the cosmetic composition of the present invention was formulated at a predetermined concentration or more and directly applied to human skin, it was found that the rough, dry skin was changed to moist and smooth skin after a certain time. This fact is a result of re-examination of skin exfoliation effect, cell proliferation effect, wound healing effect and antioxidant effect.
본 발명의 살구씨, 포도씨, 들깨로부터 유기산과 불포화 지방산의 추출방법은 다음과 같다.Extraction method of organic acid and unsaturated fatty acid from apricot seed, grape seed, perilla of the present invention is as follows.
일차로 살구씨, 포도씨, 들깨를 완전 건조한 후, 일정 건조 중량비에 대하여 추출용매로서 물, 에탄올, 메탄올, 부틸렌글리콜 및 프로필렌글리콜로 구성된 그룹으로부터 선택된 하나 이상의 용매를 5-20배 부피량을 가한다. 추출 방법으로는 냉각 콘덴서가 장치되어 용매가 증발되는 것을 방지한 상태에서 50-95℃, 4-20시간 가열하여 추출하거나 5-3℃에서 1-15일간 침적시켜 유효성분을 추출하는 방법을 사용할 수 있다. 이렇게 추출한 살구씨, 포도씨, 들깨의 혼합 추출물을 냉각 콘덴서가 달린 증류장치를 이용하여 증발되어 나오는 용매를 회수하면서 완전히 감압 농축한 후 그 건조중량으로서 0.01-10%, 바람직하게는 0.01-5%의 양으로 화장료에 첨가하는 것이다. 아래의 실시예 및 실험 예는 본 발명의 내용을 설명하나, 본 발명의 내용이 여기에 한정되지는 않는다.After first drying the apricot seed, grape seed and perilla, 5-20 times by volume of one or more solvents selected from the group consisting of water, ethanol, methanol, butylene glycol and propylene glycol as extracting solvents for a certain dry weight ratio. do. Extraction method is a method of extracting by heating at 50-95 ° C for 4-20 hours or extracting active ingredient at 5-3 ° C for 1-15 days while cooling condenser is installed to prevent solvent evaporation. Can be. The mixed extracts of apricot seed, grape seed and perilla extracted in this way are concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser, and then the dry weight thereof is 0.01-10%, preferably 0.01-5%. The amount is added to the cosmetic. The following Examples and Experimental Examples illustrate the content of the invention, but the content of the invention is not limited thereto.
본 발명의 살구씨, 포도씨, 들깨 혼합 추출물의 제조방법은 다음과 같다.Apricot seed, grape seed, perilla mixed extract of the present invention is prepared as follows.
실시예 1. 추출물 제조Example 1. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 200g, 포도씨 400g, 들깨 400g)을 증류수 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 34g(건조중량)을 얻었다.1kg (200g of apricot seed, 400g of grape seed, 400g of perilla seed) completely dried, put into 15kg of distilled water, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered with 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 34 g (dry weight).
실시예 2. 추출물 제조Example 2. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 200g, 포도씨 600g, 들깨 200g)을 에탄올 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 35g(건조중량)을 얻었다.1 kg (200 g of apricot seed, 600 g of grape seed, 200 g of perilla seed) completely dried were put in 15 kg of ethanol, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 35 g (dry weight).
실시예 3. 추출물 제조Example 3. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 400g, 포도씨 200g, 들깨 400g)을 메탄올 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 37g(건조중량)을 얻었다.1kg (400g of apricot seed, 200g of grape seed, 400g of perilla seed) completely dried were put in 15kg of methanol, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 37 g (dry weight).
실시예 4. 추출물 제조Example 4. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 300g, 포도씨 400g, 들깨 300g)을 부탄올 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 29g(건조중량)을 얻었다.1kg (300g of apricot seeds, 400g of grape seeds, 300g of perilla seeds) dried completely and put in 15kg of butanol, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 29 g (dry weight).
실시예 5. 추출물 제조Example 5. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 450g, 포도씨 300g, 들깨 250g)을 아세톤 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 32g(건조중량)을 얻었다.1kg of completely dried mixed raw materials (450g of apricot seed, 300g of grape seed, 250g of perilla) were put in 15kg of acetone, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered with 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 32 g (dry weight).
실시예 6. 추출물 제조Example 6. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 250g, 포도씨 450g, 들깨 300g)을 에틸아세테이트 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 33g(건조중량)을 얻었다.1kg (250g of apricot seed, 450g of grape seed, 300g of perilla seed) completely dried were put into 15kg of ethyl acetate, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled at 5-15 ° C. After aging for 7 days, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 33 g (dry weight).
실시예 7. 추출물 제조Example 7. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 400g, 포도씨 200g, 들깨 400g)을 헥산 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 30g(건조중량)을 얻었다.1kg (400g of apricot seeds, 200g of grape seeds, 400g of perilla seeds) completely dried and then put in 15kg of hexane, boiled for 3 hours in an extractor with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 30 g (dry weight).
실시예 8. 추출물 제조Example 8. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 500g, 포도씨 200g, 들깨 300g)을 프로판올 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 32g(건조중량)을 얻었다.1kg (500g of apricot seeds, 200g of grape seeds, 300g of perilla seeds) completely dried, put into 15kg of propanol, boiled for 3 hours in an extractor with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then cooled to 7 at 5-15 ° C. After aging for one day, it was filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 32 g (dry weight).
실시예 9. 추출물 제조Example 9. Extract Preparation
완전 건조시킨 혼합 원료 1kg(살구씨 400g, 포도씨 250g, 들깨 350g)을 함수 부틸렌글리콜 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 39g(건조중량)을 얻었다.1kg of completely dried mixed raw material (400g of apricot seed, 250g of grape seed, 350g of perilla) was put in 15kg of hydrated butylene glycol, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, 5-15 After aging for 7 days at ℃ was filtered by Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 39 g (dry weight).
실시예 10. 추출물 제조Example 10. Preparation of Extracts
완전 건조시킨 들깨 혼합 원료 1kg(살구씨 350g, 포도씨 300g, 들깨 350g)을 함수 프로필렌글리콜 15kg에 넣고 냉각 콘덴서가 달린 추출기에서 3시간 끓여서 추출한 후 400메쉬 여과포로 여과하고 상온으로 냉각한 후 5-15℃에서 7일간 방치하여 숙성시킨 후 와트만 2번 여과지로 여과 하였다. 이 혼합 식물 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하여 36g(건조중량)을 얻었다.1kg (350g of apricot seeds, 300g of grape seeds, 350g of perilla seeds) completely dried, put into 15kg of hydrous propylene glycol, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, 5-15 After aging for 7 days at ℃ was filtered by Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 36 g (dry weight).
실험 1. 항산화 효과 실험Experiment 1. Antioxidant Effect Experiment
상기 추출 혼합물 실시예 2에 대하여 항산화 효과를 측정 하였다.The antioxidant effect of the extract mixture Example 2 was measured.
실험 방법Experiment method
항산화 효과 측정은 펜톤반응 (Fenton reaction)에 의한 지질과산화 반응계(lipid peroxidation system)을 이용한 티오부틸레이트아세트산(TBA) 시험으로 측정하였다. 에틸리놀레이트 10㎕, 0.02 % 도데실황산염, 염화제이철 10㎛ol, 과산화수소 2㎛ol을 함유하는 25mM 트리스 완충용액 / 0.75mM 칼륨 완충용액 5ml에 추출 혼합물의 도데실메칠황산염 용액을 가한 후, 55℃ 에서 16 시간 배양 후, 4 %부틸히드록실톨루엔 50㎕ 를 가한 후 반응을 정지시킨다. 다음 이 용액 0.3ml을 시험관에 넣고 0.05M 염산 3.0ml, 0.67% 티오부틸레이트아세트산 용액을 가하고 30 분간 끓인다. 이 반응액을 냉각시킨 후, 85% 부탄올 4.0ml를 가하고 원심분리 후 부탄올층의 흡광도를 535nm에서 측정하여 항산화 효과(%) 를 구한다. 이때 대조시험을 동시에 실시한다. 결과를 하기 표 1 및 도 1에 나타내었다.Antioxidant effect was measured by thiobutylate acetic acid (TBA) test using a lipid peroxidation system by the Fenton reaction. To 5 ml of 25 mM Tris buffer / 0.75 mM potassium buffer containing 10 μl ethyl linoleate, 0.02% dodecyl sulfate, 10 μl ferric chloride, and 2 μol hydrogen peroxide, add dodecyl methyl sulfate solution of the extract mixture, and then 55 After 16 hours of incubation at 50 ° C, 50 µl of 4% butylhydroxytoluene was added and the reaction was stopped. 0.3 ml of this solution is added to a test tube, and 0.05 ml hydrochloric acid 3.0 ml and 0.67% thiobutylate acetic acid solution are added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and after centrifugation, the absorbance of the butanol layer was measured at 535 nm to obtain an antioxidant effect (%). At this time, the control test is performed simultaneously. The results are shown in Table 1 and FIG. 1.
표1. 살구, 포도씨, 들깨 혼합 추출물의 항산화 효과Table 1. Antioxidant Effect of Apricot, Grape Seed, and Perilla Mixed Extracts
실험예 2. 세포보호 효과 실험Experimental Example 2. Cytoprotective effect experiment
상기 추출 혼합물 중 항산화 효과가 우수한 실시예 2에 대하여 히드록실 라디칼로부터 세포보호 효과를 측정하였다.The cytoprotective effect from hydroxyl radicals was measured for Example 2 having an excellent antioxidant effect in the extract mixture.
실험 방법Experiment method
사람 섬유아세포를 2×105세포/웰 로 웰 플래이트에 놓고 10% FBS 함유하는 DMEM 배지를 0.2㎖을 공급한 후 24 시간 동안 CO2배양기에서 배양한다. 여기에 DMSO에 녹인 혼합 추출액을 처리한 다음 히드록실 라디칼을 발생시키기 위하여 30mM H2O2를 가한 후 24시간 동안 배양한다. 세포의 증식을 알아보기 위하여 MTT assay를 수행한다. MTT assay는 배양한 세포에 MTT 용액 0.1㎖를 가한 후 4시간동안 배양한 다음 배지는 제거하고 DMSO 0.5㎖를 넣어 형성된 포르마잔을 ELISA 판독기를 사용해 570㎚에서 흡광도를 측정하였다. 결과를 하기 표 2 및 도 2에 나타내었다.Human fibroblasts are placed on 2 × 10 5 cells / well low plate and fed 0.2 ml of DMEM medium containing 10% FBS and incubated in a CO 2 incubator for 24 hours. After treatment with the mixed extract dissolved in DMSO and added with 30mM H 2 O 2 to generate hydroxyl radicals and incubated for 24 hours. Perform MTT assay to determine cell proliferation. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed, and 0.5 ml of DMSO was added to formmazan. The absorbance was measured at 570 nm using an ELISA reader. The results are shown in Table 2 and FIG. 2.
표 2TABLE 2
실험예 3. 세포 증식 효과 실험Experimental Example 3. Cell proliferation effect experiment
상기 추출 혼합물 중 항산화 효과가 우수한 실시예 2에 대하여 세포증식 효과를 측정 하였다.The cell proliferation effect of Example 2, which is excellent in antioxidant effect, was measured in the extract mixture.
실험 방법Experiment method
96 마이크로웰 플래이트에 5% 어린 송아지 혈청(FBS) 용액 100ul 씩 넣은 다음, 2% FBS 가 함유된 DMEM 배지에 시료를 일정농도 분산시켜 묽혀 100ul씩 다시첨가 하였다. 혈구계를 이용하여 신생아의 포피(foreskin) 조직에서 얻은 섬유아세포를 웰 당 4000 개씩 100ul 배지에 분산시켜 가한 후 7 일간 배양 하였다. 배양이 끝난 후 상등액을 제거 하고 다시 5%PBS(인산염 완충액) 200ul 씩을 가하여 세척하고 DMSO를 웰 당 150ul 씩 가한 후 10 분간 흔들어 녹인 후 570nm 에서 흡광도를 측정 하였다. 이때 추출 혼합물을 첨가하지 않고 같은 방법으로 실험을 진행한 것을 대조군으로 하여 상대적인 흡광도의 차이로부터 세포증식 효과를 비교하였다. 결과를 하기 표 3 및 도 3에 나타내었다.100 μl of 5% young calf serum (FBS) solution was added to 96 microwell plates, and then the sample was dispersed in a predetermined concentration in DMEM medium containing 2% FBS, and diluted with 100 μl. Using a hemocytometer, fibroblasts obtained from foreskin tissue of newborns were dispersed in 100ul medium per well and cultured for 7 days. After incubation, the supernatant was removed and washed again by adding 200ul of 5% PBS (phosphate buffer), and 150ul of DMSO was added per well, and then shaken for 10 minutes to measure absorbance at 570 nm. At this time, the experiment was conducted in the same manner without adding the extraction mixture as a control group and the cell proliferation effect was compared from the difference in relative absorbance. The results are shown in Table 3 and FIG. 3.
표 3TABLE 3
실험예 4. 보습실험Experimental Example 4. Moisturizing Experiment
상기 식물 혼합 추출물 중 실시예 2 에 대하여 Courage+Khazaka 사의 각질측정기(Corneometer CM 825)를 사용하여 보습 효과를 측정하였다. 건조하고 딱딱한 각질층의 제거로 촉촉한 피부가 상층으로 올라가기 때문에 각질제거 전과 후의 보습력의 차이를 알 수 있다.The moisturizing effect of Example 2 of the plant mixture extract was measured using a corneometer (Corneometer CM 825) manufactured by Courage + Khazaka. The removal of the dry, hard dead skin layer will moisten the skin to the upper layer, so you can see the difference in the moisturizing power before and after exfoliation.
실험 방법Experiment method
20 대 여성 20 명을 대상으로 상박부 2 ×2 ㎠ , 8 구획을 물로 여러 차례 세정한 후 자극없이 수분을 제거한다. 5 분경과 후, 정제수, 30% 1,3-BG 수용액,식물 혼합 추출물 실시예 2 를 각각 2 구획에 대하여 10 ㎕ 씩 점적하여 고르게 도포한다. 10 분 후, 각질측정기를 이용하여 하나의 구획 당 5 회 측정한다. 1 구획에 대하여 5 분 간격으로 6 회 측정한다. 실내온도는 20℃, 상대습도 33% 였다.Twenty women in their 20s were washed several times with water in the upper arm 2 × 2 ㎠ and 8 sections, and then water was removed without irritation. After 5 minutes, purified water, 30% 1,3-BG aqueous solution, and plant mixed extract Example 2 were evenly applied by dropping 10 µl of each of the two compartments. After 10 minutes, measure 5 times per compartment using the keratometer. Measure 6 times at 5 minute intervals for one compartment. The room temperature was 20 ° C. and relative humidity 33%.
흡습율 (%) = 시료 도포후 각질측정값 - 시료 도포전 각질 측정값이며, 데이타 값은 20명에 대한 평균치로 나타냈다. 결과는 하기 표 4 및 도 4에 나타내었다.Moisture absorption rate (%) = keratin measurement value after sample application-Keratin measurement value before sample application, the data value was expressed as the average value for 20 people. The results are shown in Table 4 and FIG. 4.
표 4Table 4
처방예 1.Prescription Example 1.
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 유연 화장수(스킨)의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.A prescription example of a flexible skin lotion (skin) among exfoliating cosmetics containing apricot seed, grape seed and perilla mixed extract is as follows. The sample implemented here refers to the thing of Example 2.
처방예 2.Prescription Example 2.
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 영양화장수(로션)의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.A prescription example of nutrient cosmetic lotion (lotion) in the exfoliating cosmetic containing apricot seed, grape seed, and perilla mixed extract is as follows. The sample implemented here refers to the thing of Example 2.
처방예 3Prescription Example 3
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 영양크림의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.Prescription example of nutrition cream among exfoliating cosmetics containing apricot seed, grape seed, perilla mixed extract is as follows. The sample implemented here refers to the thing of Example 2.
처방예 4.Prescription Example 4.
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 맛사지 크림의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.A prescription example of a massage cream in the exfoliating cosmetic containing apricot seed, grape seed and perilla mixed extract is as follows. The sample implemented here refers to the thing of Example 2.
처방예 5.Prescription Example 5.
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 팩의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.A prescription example of a pack of exfoliating cosmetics containing apricot seed, grape seed, and perilla mixed extract is as follows. The sample implemented here refers to the thing of Example 2.
처방예 6Prescription Example 6
살구씨, 포도씨, 들깨 혼합 추출물을 함유한 각질제거용 화장료 중 유화형 화운데이션의 처방예는 다음과 같다. 여기서 실시한 시료는 실시예 2의 것을 말한다.Prescription examples of emulsified foundations in exfoliating cosmetics containing apricot seed, grape seed and perilla mixed extract are as follows. The sample implemented here refers to the thing of Example 2.
실험예 5. Silicon replicas 에 의한 피부 개선 효과 측정Experimental Example 5. Measurement of skin improvement effect by Silicon replicas
살구씨, 포도씨, 들깨 혼합 추출물의 피부 개선 효과를 보기 위해 실리콘 복제(silicon replica)를 디지털 이미지 분석으로 측정하였다.Silicone replicas were measured by digital image analysis to see the skin-improving effects of apricot seed, grape seed, and perilla mixed extracts.
실험방법Experiment method
처방예 2 를 20 명을 대상으로 8 주 동안 하루 2 번, 손등 부위에 적용하였다. 미세한 선과 주름의 측정을 위하여 실리콘 복제를 디지털 이미지 분석으로 측정하였다. 결과를 도 5에 나타내었다.Prescription Example 2 was applied to the back of the hand twice a day for 8 weeks for 20 people. Silicon replication was measured by digital image analysis for the measurement of fine lines and wrinkles. The results are shown in FIG.
실험결과Experiment result
적용 전 ; 처방예 2 적용 전에는 피부결 세선의 끊어짐이 있고 형태가 일정하지 않으며 돌출과 함몰부분이 있으며, 각질의 뭉침이 심하다.Before application; Prior to the application of Formulation Example 2, the skin texture lines were broken, the shape was not constant, there were protrusions and depressions, and keratin aggregation was severe.
적용 후 ; 처방예 2 적용 후에는 피부결 세선이 선명하고 그물눈 모양이 일정하고 돌출 혹은 함몰부분이 거의 없으며 각질층이 얇고 분포가 아주 균일해졌다.After application; After application of Formulation Example 2, the skin texture lines were clear, the shape of the mesh was constant, almost no protrusions or depressions, the stratum corneum was thin, and the distribution was very uniform.
상기 실시예에서 알 수 있는 바와 같이, 본 발명의 화장료 조성물은 피부에 과다하게 축적되어 있는 각질을 제거하고, 간혹 각질 제거에 의해 생길 수 있는 피부의 손상을 막고, 동시에 세포를 증식시켜 건강한 피부를 유지하는데 탁월한 효과가 있다.As can be seen in the above embodiment, the cosmetic composition of the present invention removes dead skin cells that are excessively accumulated in the skin, prevents skin damage that may be caused by the exfoliation, and at the same time proliferates the cells to provide healthy skin. It has an excellent effect on maintaining.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020020005655A KR20030017300A (en) | 2002-01-31 | 2002-01-31 | Cosmetic compositions containing extracts of Prunus armeniaca L, Vitis vinifera L. and Perilla frutescens var. japonica Hara |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020020005655A KR20030017300A (en) | 2002-01-31 | 2002-01-31 | Cosmetic compositions containing extracts of Prunus armeniaca L, Vitis vinifera L. and Perilla frutescens var. japonica Hara |
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| KR20030017300A true KR20030017300A (en) | 2003-03-03 |
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| KR1020020005655A KR20030017300A (en) | 2002-01-31 | 2002-01-31 | Cosmetic compositions containing extracts of Prunus armeniaca L, Vitis vinifera L. and Perilla frutescens var. japonica Hara |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005082328A1 (en) * | 2004-02-28 | 2005-09-09 | Lg Household & Health Care Ltd. | Cosmetic composition for scrubbing |
| KR100849100B1 (en) * | 2007-03-22 | 2008-07-30 | 한국콜마 주식회사 | Gel type cosmetic composition for using skin keratin removing and manufacturing method thereof |
| WO2011030989A1 (en) * | 2009-09-10 | 2011-03-17 | Amorepacific Corporation | Cosmetic composition containing hunza apricot extract |
| KR101068320B1 (en) * | 2008-09-30 | 2011-09-28 | 유한킴벌리 주식회사 | Forest relaxing skin care composition with high moisturizing and skin purifying effects |
| KR20190101550A (en) * | 2018-02-23 | 2019-09-02 | 주식회사 위노바 | Cosmetic composition comprising the extract of Hibiscus Esculentus, Chenopodium quinoa, PunicagranatumL., Perilla frutescens, Chamaecyparis obtusa, Propolis and synthetic peptide as active ingredient |
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| US5747049A (en) * | 1995-07-07 | 1998-05-05 | Shiseido Company, Ltd. | Cosmetic composition |
| KR19990070229A (en) * | 1998-02-18 | 1999-09-15 | 송시훈 | Seed Oil Manufacturing Method |
| US6045779A (en) * | 1994-02-18 | 2000-04-04 | Henkel Kommanditgesellschaft Auf Aktien | Skin and hair aerosol foam preparations containing an alkyl polyglycoside and vegetable oil |
| JP2001335417A (en) * | 2000-05-23 | 2001-12-04 | Shiyaroone:Kk | Cosmetic composition, and its pack agent and bath agent |
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| US4626529A (en) * | 1981-10-23 | 1986-12-02 | L'oreal | Oily compositions comprising a dermatological active principle, intended for the treatment of the scalp or skin |
| US6045779A (en) * | 1994-02-18 | 2000-04-04 | Henkel Kommanditgesellschaft Auf Aktien | Skin and hair aerosol foam preparations containing an alkyl polyglycoside and vegetable oil |
| US5747049A (en) * | 1995-07-07 | 1998-05-05 | Shiseido Company, Ltd. | Cosmetic composition |
| KR19990070229A (en) * | 1998-02-18 | 1999-09-15 | 송시훈 | Seed Oil Manufacturing Method |
| JP2001335417A (en) * | 2000-05-23 | 2001-12-04 | Shiyaroone:Kk | Cosmetic composition, and its pack agent and bath agent |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005082328A1 (en) * | 2004-02-28 | 2005-09-09 | Lg Household & Health Care Ltd. | Cosmetic composition for scrubbing |
| KR100849100B1 (en) * | 2007-03-22 | 2008-07-30 | 한국콜마 주식회사 | Gel type cosmetic composition for using skin keratin removing and manufacturing method thereof |
| KR101068320B1 (en) * | 2008-09-30 | 2011-09-28 | 유한킴벌리 주식회사 | Forest relaxing skin care composition with high moisturizing and skin purifying effects |
| WO2011030989A1 (en) * | 2009-09-10 | 2011-03-17 | Amorepacific Corporation | Cosmetic composition containing hunza apricot extract |
| KR101134703B1 (en) * | 2009-09-10 | 2012-04-13 | (주)아모레퍼시픽 | Cosmetic composition containing Hunza apricot extract |
| KR20190101550A (en) * | 2018-02-23 | 2019-09-02 | 주식회사 위노바 | Cosmetic composition comprising the extract of Hibiscus Esculentus, Chenopodium quinoa, PunicagranatumL., Perilla frutescens, Chamaecyparis obtusa, Propolis and synthetic peptide as active ingredient |
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