KR20020069863A - 단백질 및 다당류를 이용한 이중코팅 유산균 원말의제조방법 - Google Patents
단백질 및 다당류를 이용한 이중코팅 유산균 원말의제조방법 Download PDFInfo
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- KR20020069863A KR20020069863A KR1020010010397A KR20010010397A KR20020069863A KR 20020069863 A KR20020069863 A KR 20020069863A KR 1020010010397 A KR1020010010397 A KR 1020010010397A KR 20010010397 A KR20010010397 A KR 20010010397A KR 20020069863 A KR20020069863 A KR 20020069863A
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- protein
- lactic acid
- acid bacteria
- polysaccharide
- coating
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 136
- 241000894006 Bacteria Species 0.000 title claims abstract description 68
- 239000004310 lactic acid Substances 0.000 title claims abstract description 68
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 68
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 50
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 50
- 239000000843 powder Substances 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 230000009977 dual effect Effects 0.000 title abstract 3
- 238000000576 coating method Methods 0.000 claims abstract description 37
- 239000011248 coating agent Substances 0.000 claims abstract description 36
- 150000004676 glycans Chemical class 0.000 claims abstract description 36
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 36
- 239000005017 polysaccharide Substances 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 22
- 239000000243 solution Substances 0.000 claims abstract description 22
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 238000004108 freeze drying Methods 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 235000013372 meat Nutrition 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000012138 yeast extract Substances 0.000 claims abstract description 8
- 108091005804 Peptidases Proteins 0.000 claims abstract description 7
- 239000012460 protein solution Substances 0.000 claims abstract 2
- 239000007864 aqueous solution Substances 0.000 claims description 21
- 239000001913 cellulose Substances 0.000 claims description 17
- 229920002678 cellulose Polymers 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000002577 cryoprotective agent Substances 0.000 claims description 11
- 235000020183 skimmed milk Nutrition 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 7
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 4
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 3
- MKSMEGFVJFHTAL-UHFFFAOYSA-L dipotassium;diformate Chemical compound [K+].[K+].[O-]C=O.[O-]C=O MKSMEGFVJFHTAL-UHFFFAOYSA-L 0.000 claims description 3
- 230000002797 proteolythic effect Effects 0.000 claims description 3
- 235000002639 sodium chloride Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 abstract description 42
- 239000002253 acid Substances 0.000 abstract description 14
- 210000000941 bile Anatomy 0.000 abstract description 13
- 239000004365 Protease Substances 0.000 abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 4
- 108010073771 Soybean Proteins Proteins 0.000 abstract description 3
- 230000001766 physiological effect Effects 0.000 abstract description 2
- 239000003223 protective agent Substances 0.000 abstract description 2
- 235000019710 soybean protein Nutrition 0.000 abstract 2
- 108010011756 Milk Proteins Proteins 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 235000021239 milk protein Nutrition 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 23
- 230000008569 process Effects 0.000 description 13
- 230000004083 survival effect Effects 0.000 description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 241000186660 Lactobacillus Species 0.000 description 8
- 229940039696 lactobacillus Drugs 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000003756 stirring Methods 0.000 description 7
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 5
- 229930195725 Mannitol Natural products 0.000 description 5
- 238000007710 freezing Methods 0.000 description 5
- 230000008014 freezing Effects 0.000 description 5
- 239000000594 mannitol Substances 0.000 description 5
- 235000010355 mannitol Nutrition 0.000 description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 239000005913 Maltodextrin Substances 0.000 description 4
- 229920002774 Maltodextrin Polymers 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 229940035034 maltodextrin Drugs 0.000 description 4
- 235000004252 protein component Nutrition 0.000 description 4
- 239000008199 coating composition Substances 0.000 description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 235000020130 leben Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 108010043393 protease N Proteins 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- 241001608472 Bifidobacterium longum Species 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 241000194020 Streptococcus thermophilus Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229940009291 bifidobacterium longum Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011814 protection agent Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003613 bile acid Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000002702 enteric coating Substances 0.000 description 1
- 238000009505 enteric coating Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000008570 general process Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 230000008991 intestinal motility Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940066779 peptones Drugs 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 229940001941 soy protein Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
내산성 시험(pH2.1의 인공위액 중) | 내담즙성 시험(0.5% Oxgall 용액 중) | 가속시험(40℃, 70% 상대습도) | ||||||
노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 경과시간(일) | 시험구(cfu/g) | 대조구(cfu/g) |
0 | 3.4x1011 | 3.3x1011 | 0 | 3.5x1011 | 3.4x1011 | 0 | 3.1x1011 | 3.2x1011 |
30 | 3.2x1011 | 2.3x1011 | 30 | 3.3x1011 | 2.1x1011 | 10 | 2.9x1011 | 1.1x1011 |
60 | 3.1x1011 | 1.5x109 | 60 | 3.2x1011 | 1.4x1011 | 20 | 2.6x1011 | 8.9x1010 |
90 | 3.1x1011 | 1.2x1011 | 90 | 3.0x1011 | 1.1x1011 | 30 | 2.5x1011 | 1.0x1010 |
120 | 2.9x1011 | 9.3x1011 | 120 | 2.9x1011 | 9.9x1010 | 40 | 2.0x1011 | 9.8x109 |
120분경과시의 생존율(%) | 85.3 | 28.2 | 120분경과시의 생존율(%) | 82.9 | 29.1 | 40일경과시의생존율(%) | 64.5 | 3.1 |
내산성 시험(pH2.1의 인공위액 중) | 내담즙성 시험(0.5% Oxgall 용액 중) | 가속시험(40℃, 70% 상대습도) | ||||||
노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 경과시간(일) | 시험구(cfu/g) | 대조구(cfu/g) |
0 | 2.0x1011 | 2.2x1011 | 0 | 2.7x1011 | 2.9x1011 | 0 | 2.6x1011 | 2.7x1011 |
30 | 1.9x1011 | 1.7x1011 | 30 | 2.7x1011 | 2.3x1011 | 10 | 2.5x101 | 1.3x1011 |
60 | 1.8x1011 | 1.2x1011 | 60 | 2.5x1011 | 1.9x1011 | 20 | 2.5x1011 | 9.7x1010 |
90 | 1.8x1011 | 9.5x1010 | 90 | 2.4x1011 | 1.5x1010 | 30 | 2.4x1011 | 8.6x1010 |
120 | 1.8x1011 | 8.4x1010 | 120 | 2.4x111 | 9.2x1010 | 40 | 2.2x1011 | 5.1x1010 |
120분경과시의 생존율(%) | 90.0 | 38.2 | 120분경과시의 생존율(%) | 88.9 | 31.7 | 40일경과시의생존율(%) | 84.6 | 18.9 |
내산성 시험(pH2.1의 인공위액 중) | 내담즙성 시험(0.5% Oxgall 용액 중) | 가속시험(40℃, 70% 상대습도) | ||||||
노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 노출시간(분) | 시험구(cfu/g) | 대조구(cfu/g) | 경과시간(일) | 시험구(cfu/g) | 대조구(cfu/g) |
0 | 4.0x1011 | 3.8x1011 | 0 | 3.7x1011 | 3.6x1011 | 0 | 3.6x1011 | 4.0x1011 |
30 | 3.9x1011 | 2.3x1011 | 30 | 3.5x1011 | 2.6x1011 | 10 | 3.3x1011 | 1.1x1011 |
60 | 3.8x1011 | 2.1x1011 | 60 | 3.4x1011 | 2.3x1011 | 20 | 2.9x1011 | 9.3x1010 |
90 | 3.8x1011 | 1.3x1010 | 90 | 3.2x1011 | 1.9x1011 | 30 | 2.7x1011 | 3.7x1010 |
120 | 3.5x1011 | 9.9x1010 | 120 | 3.0x1011 | 9.8x1010 | 40 | 2.3x1011 | 1.4x1010 |
120분경과시의 생존율(%) | 87.5 | 26.1 | 120분경과시의 생존율(%) | 81.3 | 27.2 | 40일경과시의생존율(%) | 63.9 | 3.5 |
Claims (4)
- 대두분리단백과 탈지분유를 소정의 혼합비로 혼합하여 1%-10%수용액으로 만든 단백질 수용액을 유산균주에 따라서 대단백질 중량비 0.01%-1%범위의 단백분해 효소용액을 가하여 효소처리하는 단백질효소분해단계와;상기 효소처리액에 중량비 1%-5%범위의 포도당, 0.1%-1.5%범위의 효모추출물, 0.1%-1.5%범위의 육엑기스, 0.01%-0.1%범위의 이온성분을 첨가하여 용해시키고 발효관에서 스팀살균 후 유산균을 배양하는 유산균 발효단계와;상기 발효된 발효액을 고속원심분리기에 의해 분리 농축하여 균체를 분리하고 코팅하는 1차 단백질코팅단계와;상기 회수된 균체에 대비하여 중량비 1%-10%의 동결보호제 성분으로 조성한 35%-45% 동결보호제 수용액과, 회수균체 대비 1%-10%의 다당류 성분으로 조성한 1%-10% 다당류 수용액을 혼합 사용하여 코팅하고 동결건조하는 2차코팅단계를 포함하는 것을 특징으로 하는 단백질 및 다당류를 이용한 이중코팅 유산균 원말의 제조방법.
- 제 1항에 있어서,상기 이온성분으로 암모니움 시트레이트, 소디움 아세테이트, 디포타시움 포스테이트, 마그네슘 설페이트, 망간 설페이트, 소디움 클로라이드의 이온성분을 사용하는 것을 특징으로 하는 단백질 및 다당류를 이용한 이중코팅 유산균 원말의 제조방법.
- 제 1항에 있어서,상기 다당류 수용액으로 잔탐검(Xantan gum), 셀룰로즈(Cellulose), 리벤(Levan)을 사용하는 것을 특징으로 하는 단백질 및 다당류를 이용한 이중코팅 유산균 원말의 제조방법.
- 제 1항에 있어서,상기 1차 단백질코팅단계에 의하여 제조된 유산균 원말에 대하여 2차 다당류 코팅을 실시하고, 동결건조하여 제조하는 것을 특징으로 하는 단백질 및 다당류를 이용한 이중코팅 유산균 원말의 제조방법.
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JP2002054821A JP3720780B2 (ja) | 2001-02-28 | 2002-02-28 | 蛋白質及び多糖類を利用した二重コーティング乳酸菌原末の製造方法 |
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KR100782984B1 (ko) * | 2006-04-21 | 2007-12-07 | (주)케비젠 | 유산균 다중 마이크로캡슐의 제조방법, 이 방법에 의해제조된 마이크로캡슐 및 이를 포함하는 제품 |
WO2009093785A1 (en) * | 2008-01-25 | 2009-07-30 | Cell Biotech Co., Ltd. | Method of preparing triple-coating lactic acid bacteria and nano particle coating method, triple-coating lactic acid bacteria prepared thereby and article comprising the same |
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JP2968089B2 (ja) * | 1991-04-25 | 1999-10-25 | 鐘紡株式会社 | 被覆用素材及びそれを用いた腸溶性造粒物 |
JP3202053B2 (ja) * | 1992-01-09 | 2001-08-27 | カネボウ株式会社 | 腸溶性造粒物 |
KR970025405A (ko) * | 1997-03-18 | 1997-06-24 | 조광현 | 유산균 미세 캡슐의 제조방법 |
KR100263822B1 (ko) * | 1997-10-14 | 2000-11-01 | 신종훈 | 유산균 캡슐제 요구르트 및 그 제조방법 |
KR100387245B1 (ko) * | 1997-10-17 | 2003-08-19 | 일양약품주식회사 | 유산균의안정화를위한미세장용성코팅과립 |
KR20000011247A (ko) * | 1998-07-23 | 2000-02-25 | 김윤 | 다당류를이용한대장선택성약물전달조성물및약학제제 |
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2001
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KR100782984B1 (ko) * | 2006-04-21 | 2007-12-07 | (주)케비젠 | 유산균 다중 마이크로캡슐의 제조방법, 이 방법에 의해제조된 마이크로캡슐 및 이를 포함하는 제품 |
WO2009093785A1 (en) * | 2008-01-25 | 2009-07-30 | Cell Biotech Co., Ltd. | Method of preparing triple-coating lactic acid bacteria and nano particle coating method, triple-coating lactic acid bacteria prepared thereby and article comprising the same |
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KR102009731B1 (ko) * | 2019-04-15 | 2019-08-12 | 주식회사 쎌바이오텍 | 단백질 가수분해물을 이용한 단백질-다당류 이중코팅 유산균의 제조방법 |
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KR100429495B1 (ko) | 2004-05-03 |
JP3720780B2 (ja) | 2005-11-30 |
JP2002320473A (ja) | 2002-11-05 |
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