KR20010097642A - Method for Manufacturing of Mineral Fortified Sea-Cucumber Extract - Google Patents

Method for Manufacturing of Mineral Fortified Sea-Cucumber Extract Download PDF

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KR20010097642A
KR20010097642A KR1020000021902A KR20000021902A KR20010097642A KR 20010097642 A KR20010097642 A KR 20010097642A KR 1020000021902 A KR1020000021902 A KR 1020000021902A KR 20000021902 A KR20000021902 A KR 20000021902A KR 20010097642 A KR20010097642 A KR 20010097642A
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정현종
박근종
박형석
김박
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김박
앨트웰텍 주식회사
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Abstract

본 발명은 해삼을 효소로 처리하여 단백질을 분해한 후, 그 분해산물에 각종 미네랄을 첨가하여 해삼에 존재하지 않거나 부족한 무기 성분을 강화함으로써, 해삼이 가지고 있는 영양학적 및 약리학적 기능을 향상시킨 미네랄 강화 해삼 추출물의 제조방법에 관한 것이다.The present invention is a mineral that enhances the nutritional and pharmacological functions of sea cucumbers by treating sea cucumbers with enzymes to decompose proteins, and then by adding various minerals to the decomposition products to reinforce inorganic components not present or lacking in sea cucumbers. It relates to a method of preparing fortified sea cucumber extract.

본 발명에 의하여 제조되는 해삼추출물에는 아연을 비롯한 각종 필수 미네랄 성분이 강화됨으로써 전립선 기능 향상, 관절염 개선, 면역력 증강, 호르몬 합성 능력 강화, 지방대사 능력 강화 등의 효과가 더욱 향상될 수 있다.Sea cucumber extract prepared by the present invention may be enhanced by various essential mineral components, including zinc, thereby improving the effects of prostate function, arthritis, immunity, hormonal synthesis, and fat metabolism.

Description

미네랄 강화 해삼추출물의 제조방법 {Method for Manufacturing of Mineral Fortified Sea-Cucumber Extract}Method for Manufacturing of Mineral Fortified Sea-Cucumber Extract

고대 이래 해삼을 식용으로 사용한 예는 많으나 해삼이 가지고 있는 영양학적 분석 및 연구는 19세기 이후인 근대에 와서야 이루어지기 시작했다.Since ancient times, sea cucumbers have been used for food, but the nutritional analysis and researches of sea cucumbers did not begin until the 19th century.

한국, 중국을 비롯한 동양에서는 옛부터 해삼이 진액을 보하는 약으로 당뇨병, 천식 등에 효과가 탁월하고 여름에 온도 상승으로 땀을 많이 흘려 기력상실과 허탈상태에 빠졌을 때 기력을 되찾을 수 있는 약으로 알려져 있다. 해삼에 관한 한국의 옛 문헌을 보면, [물보]에서는 물에 사는 생물족으로 분류하고 "해남자(海男)"라고 한 바 있으며, [전어지]에서는 "해삼은 성이 온하고 몸을 보하는 바 그 효력이 인삼과 맞먹는다"고 한 바 있고, [자산어보]에서는 해삼이 전복, 홍합과 함께 세가지 재물중 하나라고 한 바 있으며, [규합총서]에는 열구자탕과 어채로 해삼을 사용하였다는 기록이 있다.In Korea, China, and the East, sea cucumbers have been used as a supplement for diabetes and asthma, and they can regain energy when they lose energy and collapse due to sweating due to temperature rise in summer. Known. In the old Korean literature on sea cucumbers, in the [People], they were classified as aquatic species living in the water, and they were called "sea men." Its effect is equivalent to ginseng. ”In the Asset Asset Report, sea cucumber is one of three riches along with abalone and mussel. have.

해삼은 몸의 일부가 절단되었을 경우 3개월만에 절단부위가 원상 복귀되고, 내장이 제거되어도 1개월만에 새로운 내장이 생기는 놀라운 복원력을 가지고 있다. 따라서, 한방과 민간요법에서는 해삼이 인체 단핵세포와 거식세포의 담식능력을 제고시켜 면역기능을 왕성하게 하고 상처의 치료에 효과가 있는 것으로 알려져 있다. 또한, 현대과학에 의하여 해삼에 단백질과 비타민 B류가 풍부하고 요오드 성분이 많이 함유되어 있으며, 각종 식품 및 의약품의 원료로 사용되는 콘드로이틴황산이 다량 함유되어 있음이 이미 공지되어 있다.Sea cucumbers have a remarkable resilience effect that when a part of the body is cut, the cut site returns to its original state in three months, and new intestines are formed in one month even if the intestines are removed. Therefore, in oriental medicine and folk remedies, sea cucumber is known to be effective in the treatment of wounds by enhancing the immune function of human monocytes and macrophages. In addition, it is already known by modern science that sea cucumber is rich in protein and vitamin B and contains iodine, and contains a large amount of chondroitin sulfate, which is used as a raw material for various foods and medicines.

이렇게 식용 및 약용으로 겸용되고 있는 해삼은 바다의 인삼으로 불리어져 온 수산 건강식품으로서 그 동안 자양강장과 보신용 식품으로 널리 사용되어 왔는데, 한국 특허등록 제 10-0244564호에 해삼을 볏짚과 혼합하여 발효시키고 그 발효물을 여과하여 얻은 여액을 분말화한 발효식품이 개시된 바 있으며, 한국 특허등록 제 10-0167831호에는 해삼을 수용액 상에서 추출한 후 알콜로 분별 침전시켜 콘드로이틴황산 함유 추출물을 식품, 화장품 및 의약품의 원료로 사용하는 방법이 개시된 바도 있다.The sea cucumber, which is used for food and medicinal purposes, has been widely used as a nourishment tonic and bosin food, which has been called sea ginseng. It is fermented by mixing sea cucumber with rice straw in Korean Patent Registration No. 10-0244564 The fermented food is powdered filtrate obtained by filtration of the fermented product, and Korean Patent Registration No. 10-0167831 discloses extracting sea cucumber in an aqueous solution and then precipitating with alcohol to extract the extract containing chondroitin sulfate. The method of using as a raw material has also been disclosed.

이와 같은 해삼의 생체 구성 성분을 살펴보면, 수분 90.5±1.5%, 단백질 3.2±1.4%, 지방 0.3±0.2%, 탄수화물 2.2±0.2%, 회분 3.5±0.2% 등을 비롯하여 각종 미네랄류와 비타민으로 이루어져 있으며, 이러한 성분들의 조성은 해삼의 계절적인 식이습관의 차이, 개체연령과 서식지역의 차이에 따라 변동폭이 심하게 나타나는데(Castell 등 1966, Dhew 1972, Mottet 1976, Sidwell 1981), 일반적으로생 해삼을 건조하면 단백질 30%, 지방 3.5%, 탄수화물 25%, 회분 36%의 성분을 가지게 된다.The bio components of sea cucumbers are composed of various minerals and vitamins including 90.5 ± 1.5% moisture, 3.2 ± 1.4% protein, 0.3 ± 0.2% fat, 2.2 ± 0.2% carbohydrate, 3.5 ± 0.2% ash, etc. The composition of these components varies greatly depending on the seasonal dietary habits of sea cucumbers, and the differences in population age and habitat (Castell et al. 1966, Dhew 1972, Mottet 1976, Sidwell 1981). It will contain 30% fat, 3.5% fat, 25% carbohydrate, and 36% ash.

한편, 종래 생 해삼을 건조하는 방법을 살펴보면, 한국 특허공고 제 97-7938호 및 제 92-4448호 에서 각각 생 해삼을 단백질 응고제로서 암모니아수에 침지하는 방법 및 pH 4-6의 산성용액에 침지하여 건조하는 방법을 택함으로써, 해삼의 원형을 보기 좋게 보존하기는 하였으나, 식용시 소화 흡수를 하는 데에는 효과가 별로 개선된 바 없었으며, 한국 출원 제 99-0003595호에 의한 해삼 펩타이드의 제조방법에서는 일반적인 분해효소로 처리하여 해삼에 있는 단백질만을 분해한 바 있는데, 이와 같이 해삼 원형을 보존하거나 단백질 성분을 단순 분해하는 것이 이제까지의 해삼 가공의 수준이었다.On the other hand, the conventional method of drying raw sea cucumber, in Korean Patent Publication Nos. 97-7938 and 92-4448, the method of dipping raw sea cucumber as a protein coagulant in ammonia water and acidic solution of pH 4-6, respectively, Although the original form of sea cucumber was preserved by the method of drying, the effect of digestion and absorption during edible was not much improved, and the method of preparing sea cucumber peptides according to Korean Application No. 99-0003595 was common. Decomposing enzymes and digested only the proteins in sea cucumbers, such as preservation of the sea cucumber prototype or simple decomposition of protein components was the level of sea cucumber processing so far.

그러나, 해삼이 함유하고 있는 미네랄을 구체적으로 살펴보면, 상기한 요오드 이외에도 칼슘이 134mg/100g, 인이 24mg/100g 으로 다량 함유되어 있으나 K, Zn, Mg, Cu, Fe 등은 상대적으로 미량 함유하고 있는데(Teeny 등 1984, Gordon 등 1977, 최 등 1985, 양 등 1982, Anthony 등 1983, Lee 등 1994, 小島 등 1990), 이중에서도 특히 아연은 전립선 기능의 개선, 관절염 증상의 개선, 지방대사의 개선에 의한 여드름 발생 방지, 면역체계의 증강, 호르몬의 합성, 단백질 및 핵산의 합성 등에 필수적인 것으로 알려져 있는 미네랄이다.However, if you look specifically at the minerals contained in sea cucumber, in addition to the iodine described above, calcium, 134mg / 100g, phosphorus 24mg / 100g contains a large amount, but K, Zn, Mg, Cu, Fe, etc. (1984, etc., including Teeny, 1977, Gordon, 1977, Choi, 1982, Anthony, 1983, Lee, 1994, Koshima, etc.), among others, zinc has been shown to improve prostate function, arthritis symptoms, and fat metabolism. It is a mineral known to be essential for preventing acne from occurring, strengthening the immune system, synthesizing hormones, synthesizing proteins and nucleic acids.

이에 따라 해삼을 식용 또는 약용을 목적으로 가공하고자 하는 경우에는 이와 같은 부족 원소들을 강화시켜 줌으로써 그 효능을 극대화시킬 필요가 있게 된다. 또한, 해삼을 그대로 복용할 경우 분자량이 큰 관계로 이를 소화 흡수시키는비율이 낮으므로 생 해삼 가공시 이점 역시 보완할 필요가 있다고 볼 수 있다.Accordingly, when the sea cucumber is to be processed for edible or medicinal purposes, it is necessary to maximize the efficacy by strengthening such shortage elements. In addition, if the sea cucumber is taken as it is because the molecular weight is large, the rate of digestion and absorption is low, so the benefits of raw sea cucumber processing need to be supplemented.

본 발명의 목적은 상기와 같은 종래 생 해삼 가공 기술의 문제점들을 해결함으로써 해삼이 원래 함유하고 있는 유효성분들, 구체적으로 요오드, 칼슘, 인, 콘드로이틴황산 이외에 해삼에 없거나 부족한 성분들, 구체적으로 K, Zn, Cu, Mg, Fe 등의 미네랄을 강화시킨 해삼추출물의 제조방법을 제공하고자 하는 것이다.An object of the present invention is to solve the problems of the conventional raw sea cucumber processing technology as described above, active ingredients originally contained in sea cucumber, specifically iodine, calcium, phosphorus, chondroitin sulfate in addition or lacking components, specifically K, Zn To provide a method for producing sea cucumber extracts, minerals such as Cu, Mg, Fe and the like.

이를 위하여 본 발명은 세척한 해삼을 건조한 후 분쇄하고, 탄산칼륨 또는 탄산나트륨을 첨가하여 해삼 분말을 용해시키고, 효소를 이용하여 가수분해하고, 효소를 실활시킨 후 유기산으로 중화하고, 여과하여 거대분자 단백질을 제거하고, 적당한 미네랄을 첨가하고, 재여과한 후 농축 및 균질화하고 건조함으로써 미네랄 강화 해삼추출물을 제조하는 방법을 제공하는 것으로 이루어진다.To this end, the present invention is dried and pulverized washed sea cucumber, dissolved in sea cucumber powder by the addition of potassium carbonate or sodium carbonate, hydrolyzed using the enzyme, neutralized with organic acid after inactivating the enzyme, filtered and macromolecular protein It is to provide a method for producing a mineral-enhanced sea cucumber extract by removing the, adding the appropriate minerals, re-filtration and then concentrated and homogenized and dried.

도 1은 본 발명에 따른 미네랄 강화 해삼추출물의 제조방법을 보인 공정도.1 is a process chart showing a method for producing mineral-enriched sea cucumber extract according to the present invention.

본 발명은 해삼을 효소로 처리하여 단백질을 분해한 후, 그 분해산물에 각종 미네랄을 첨가하여 해삼에 존재하지 않거나 부족한 무기 성분을 강화함으로써, 해삼이 가지고 있는 영양학적 및 약리학적 기능을 향상시킨 미네랄 강화 해삼 추출물의 제조방법에 관한 것이다.The present invention is a mineral that enhances the nutritional and pharmacological functions of sea cucumbers by treating sea cucumbers with enzymes to decompose proteins, and then by adding various minerals to the decomposition products to reinforce inorganic components not present or lacking in sea cucumbers. It relates to a method of preparing fortified sea cucumber extract.

이하 도 1에 나타낸 공정도를 참조하여 본 발명에 따른 미네랄 강화 해삼추출물의 제조방법을 상세하게 설명하고자 하나 본 발명을 이에 제한하고자 하는 것은 아니다.Hereinafter, a method for preparing mineral-enriched sea cucumber extract according to the present invention will be described in detail with reference to the process diagram shown in FIG. 1, but the present invention is not intended to be limited thereto.

먼저, 깨끗하게 세척하여 이물질을 제거한 양질의 해삼 원물을 수분 함량 7% 이하가 되도록 40∼60℃의 진공건조기에서 5∼12시간 건조한 후, 건조된 해삼을 분쇄기에서 40 mesh 정도로 분쇄하여 분말화한다. 탄산칼륨 또는 탄산나트륨을 첨가하여 pH를 8.8∼9.8로 조정한 수용액에 이 분말을 투입하고, 70∼80℃의 온도를 유지하면서 2∼10시간 교반한다. 이와 같이 가열 및 교반됨으로써 최대한 분산된 해삼농축액을 100℃에서 30분간 살균처리한다.First, clean sea cucumber raw material that has been washed clean to remove foreign matters and dried in a vacuum dryer at 40 to 60 ° C. for 5 to 12 hours so as to have a water content of 7% or less, and then, the dried sea cucumber is pulverized in a grinder to about 40 mesh and powdered. The powder is added to an aqueous solution in which potassium carbonate or sodium carbonate is added to adjust the pH to 8.8 to 9.8, followed by stirring for 2 to 10 hours while maintaining a temperature of 70 to 80 ° C. The sea cucumber concentrate dispersed as much as possible by heating and stirring is sterilized at 100 ° C. for 30 minutes.

이렇게 살균처리된 해삼농축액을 40∼60℃로 냉각시킨 후, 단백질 가수분해 효소(Alcalose)로 40∼60℃의 온도를 유지하면서 3∼5시간 동안 가수분해시킨다. 가수분해된 해삼농축액을 100℃에서 20분간 처리하여 효소를 실활시킨 후, 젖산(Lactic acid) 또는 초산(Acetic acid)과 같은 유기산을 사용하여 pH가 6.5∼7.0이 되도록 중화시킨다.The sterilized sea cucumber concentrate is cooled to 40-60 ° C., and then hydrolyzed for 3-5 hours while maintaining a temperature of 40-60 ° C. with proteolytic enzymes (Alcalose). The hydrolyzed sea cucumber concentrate was inactivated by treatment at 100 ° C. for 20 minutes, and then neutralized to pH 6.5-7.0 using an organic acid such as lactic acid or acetic acid.

중화된 해삼농축액을 필터로 여과하여 저분자화되지 않은 불순물을 제거한 다음 50∼60℃의 온도를 유지하면서, 해삼농축액 기준으로 ZnO를 1.5∼5%, MgCl2를 0.3∼4%, 유청칼슘을 4.5∼15%, CaCO3를 1.5∼18%, FeSO4를 0.5∼15%, 젖산철을 0.2∼14% 첨가하여 용해시킨다.The neutralized sea cucumber concentrate was filtered through a filter to remove impurities that were not low molecular weight, and then maintained at a temperature of 50 to 60 ° C., based on the sea cucumber concentrate, 1.5-5% ZnO, 0.3-4% MgCl 2 and 4.5 whey calcium to 15%, 1.5~18% of CaCO 3, 0.5~15% of FeSO 4, is dissolved by the addition of lactic acid iron 0.2~14%.

미네랄이 첨가된 해삼농축액을 재여과한 후, 60∼65℃의 온도를 유지하면서 35Brix∼45Brix(35∼45%W/W)로 진공농축(진공도 600∼650mmHg)하고, 균질기에서 100∼150kg/cm2의 압력으로 균질화시킨다.After re-filtering the sea cucumber concentrate with minerals, while maintaining the temperature of 60 ~ 65 ℃ Vacuum concentration (vacuum degree 600-650mmHg) with 35Brix ~ 45Brix (35 ~ 45% W / W), 100 ~ 150kg / cm in homogenizer2Homogenize at pressure.

균질화가 완료된 해삼농축액을 약 70℃의 온도에서 분무건조(Spray drying) 또는 동결건조(Freeze drying) 함으로써 미네랄 강화 해삼 농축물을 제조한다.Mineral-enriched sea cucumber concentrate is prepared by spray drying or freeze drying the homogenized sea cucumber concentrate at a temperature of about 70 ° C.

상기와 같이 본 발명이 완성되면서, 해삼을 분해한 분해산물에 각종 미네랄을 첨가하여 해삼에 존재하지 않거나 부족한 무기 성분을 강화함으로써, 해삼이 가지고 있는 영양학적 및 약리학적 기능을 향상시킨 미네랄 강화 해삼추출물의 제조방법이 제공될 수 있게 되었다.As the present invention is completed as described above, by adding various minerals to the decomposition product that decomposed sea cucumber, mineral-enriched sea cucumber extract which improves the nutritional and pharmacological functions of sea cucumber by strengthening inorganic components not present or lacking in sea cucumber. The manufacturing method of can be provided.

또한, 본 발명이 완성됨으로써 전립선 기능 향상, 관절염 개선, 면역력 증강, 호르몬 합성 능력 강화, 지방대사 능력 강화 등의 효과가 더욱 향상된 해삼추출물이 제공되어 식품학적 또는 약학적 목적으로 사용될 수 있게 되었다.In addition, since the present invention is completed, sea cucumber extracts, which have improved effects such as improved prostate function, arthritis, immunity, hormone synthesis, and fat metabolism, have been provided to be used for food or pharmaceutical purposes.

Claims (4)

미네랄 강화 해삼추출물의 제조방법에 있어서, 세척하여 이물질을 제거한 해삼 원물을 수분 함량 7% 이하가 되도록 40∼60℃의 진공건조기에서 5∼12시간 건조한 후, 건조된 해삼을 분쇄기에서 40 mesh 정도로 분쇄하여 분말화하고, 탄산칼륨 또는 탄산나트륨을 첨가하여 pH를 8.8∼9.8로 조정한 수용액에 이 분말을 투입하고, 70∼80℃의 온도를 유지하면서 2∼10시간 교반하고, 분산된 해삼농축액을 100℃에서 30분간 살균처리하고, 살균처리된 해삼농축액을 40∼60℃로 냉각시킨 후, 단백질 가수분해 효소(Alcalose)로 40∼60℃의 온도를 유지하면서 3∼5시간 동안 가수분해시키고, 가수분해된 해삼농축액을 100℃에서 20분간 처리하여 효소를 실활시킨 후, 유기산을 사용하여 pH가 6.5∼7.0이 되도록 중화시키고, 중화된 해삼농축액을 필터로 여과하여 저분자화되지 않은 불순물을 제거한 후, 50∼60℃의 온도를 유지하면서 미네랄을 첨가하여 용해시키고, 미네랄이 첨가된 해삼농축액을 재여과한 후, 60∼65℃의 온도를 유지하면서 35Brix∼45Brix(35∼45%W/W)로 진공농축(진공도 600∼650mmHg)하고, 균질기에서 100∼150kg/cm2의 압력으로 균질화시킨 후, 균질화가 완료된 해삼농축액을 65℃∼75℃의 온도에서 분무건조(Spray drying) 하거나 -35℃∼-45℃의 온도에서 동결건조(Freeze drying) 하는 것으로 이루어짐을 특징으로 하는, 미네랄 강화 해삼추출물의 제조방법.In the method of producing mineral-enriched sea cucumber extract, the sea cucumber raw material, which has been washed and removed, is dried for 5 to 12 hours in a vacuum dryer at 40 to 60 ° C. so as to have a water content of 7% or less, and then the dried sea cucumber is ground in a grinder to about 40 mesh. Powder was added thereto, potassium carbonate or sodium carbonate was added, and the powder was added to an aqueous solution adjusted to pH 8.8 to 9.8. The mixture was stirred for 2 to 10 hours while maintaining a temperature of 70 to 80 ° C., and the dispersed sea cucumber concentrate was 100 After sterilization at 30 ° C. for 30 minutes, the sterilized sea cucumber concentrate was cooled to 40 to 60 ° C., and then hydrolyzed for 3 to 5 hours while maintaining a temperature of 40 to 60 ° C. with proteinase. The degraded sea cucumber concentrate was treated for 20 minutes at 100 ° C to inactivate the enzyme, and then neutralized to pH 6.5-7.0 using an organic acid, and the neutralized sea cucumber concentrate was filtered through a filter to prevent low molecular weight. After the impurities are removed, the minerals are added and dissolved while maintaining the temperature of 50-60 ° C., and the sea cucumber concentrate added with the minerals is re-filtered, followed by 35Brix-45Brix (35-45%) while maintaining the temperature of 60-65 ° C. W / W) vacuum concentration (vacuum degree 600-650mmHg), homogenized in a homogenizer at a pressure of 100 ~ 150kg / cm 2 , and then the homogenized sea cucumber concentrate is spray dried at a temperature of 65 ℃ ~ 75 ℃ ) Or freeze drying at a temperature of -35 ° C to -45 ° C. 제 1항에 있어서,The method of claim 1, 첨가되는 미네랄은 ZnO, MgCl2,유청칼슘, CaCO3,FeSO4및 젖산철 임을 특징으로 하는, 미네랄 강화 해삼추출물의 제조방법.Minerals to be added are ZnO, MgCl 2, whey calcium, CaCO 3, FeSO 4 and iron lactate, characterized in that the preparation of mineral-enriched sea cucumber extract. 제 1항 또는 2항에 있어서,The method according to claim 1 or 2, 첨가되는 미네랄은 해삼농축액 기준으로 ZnO가 1.5∼5%, MgCl2가 0.3∼4%, 유청칼슘이 4.5∼15%, CaCO3가 1.5∼18%, FeSO4가 0.5∼15%, 젖산철이 0.2∼14%의 양으로 첨가됨을 특징으로 하는, 미네랄 강화 해삼추출물의 제조방법.Minerals added are 1.5-5% ZnO, 0.3-4% MgCl 2 , 4.5-15% Whey Calcium, 1.5-18% CaCO 3 , 0.5-15% FeSO 4 , 0.2 Lactate Method for producing a mineral-enhanced sea cucumber extract, characterized in that added in an amount of ˜14%. 제 1항에 있어서,The method of claim 1, 유기산은 젖산(Lactic acid) 또는 초산(Acetic acid) 임을 특징으로 하는, 미네랄 강화 해삼추출물의 제조방법.Organic acid is lactic acid (Lactic acid) or acetic acid (Acetic acid), characterized in that the production method of mineral-enriched sea cucumber extract.
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KR20070105714A (en) * 2006-04-27 2007-10-31 이주석 Method for preparation of capsule of seacucumber peptide
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