KR102613142B1 - Nutrient Solution Manufacturing Method For Plant Cultivation Use Microbial Culture Solution - Google Patents
Nutrient Solution Manufacturing Method For Plant Cultivation Use Microbial Culture Solution Download PDFInfo
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 57
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 238000009629 microbiological culture Methods 0.000 title abstract description 4
- 241000196324 Embryophyta Species 0.000 claims abstract description 37
- 244000005700 microbiome Species 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 230000008635 plant growth Effects 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims description 13
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 12
- 239000011591 potassium Substances 0.000 claims description 12
- 229910052700 potassium Inorganic materials 0.000 claims description 12
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 11
- 239000011574 phosphorus Substances 0.000 claims description 11
- 229910052698 phosphorus Inorganic materials 0.000 claims description 11
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 10
- 239000011575 calcium Substances 0.000 claims description 9
- 239000000460 chlorine Substances 0.000 claims description 8
- 239000011777 magnesium Substances 0.000 claims description 8
- 239000011572 manganese Substances 0.000 claims description 8
- 239000011701 zinc Substances 0.000 claims description 8
- 239000010949 copper Substances 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 241000194103 Bacillus pumilus Species 0.000 claims description 5
- 241000278457 Bacillus siamensis Species 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 4
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- 229910052796 boron Inorganic materials 0.000 claims description 4
- 229910052801 chlorine Inorganic materials 0.000 claims description 4
- 239000010941 cobalt Substances 0.000 claims description 4
- 229910017052 cobalt Inorganic materials 0.000 claims description 4
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 229910052748 manganese Inorganic materials 0.000 claims description 4
- 229910052750 molybdenum Inorganic materials 0.000 claims description 4
- 239000011733 molybdenum Substances 0.000 claims description 4
- 229910052759 nickel Inorganic materials 0.000 claims description 4
- 239000011593 sulfur Substances 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- 229910052725 zinc Inorganic materials 0.000 claims description 4
- 235000012055 fruits and vegetables Nutrition 0.000 abstract description 9
- 230000012010 growth Effects 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012364 cultivation method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 241000208340 Araliaceae Species 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/60—Heating or cooling during the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Wood Science & Technology (AREA)
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- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
본 발명은 미생물을 이용하여 각종 꽃 또는 과채류 식물을 수경재배 방식으로 건강하게 재배할 수 있도록 한 미생물배양액을 이용한 식물재배용 양액 제조방법에 관한 것으로, 그 구성은, 물, 식물성장에 필요한 미생물, 미생물이 먹고 성장하는데 필요한 배지를 혼합하여 베이스 액을 제조하는 단계; 상기 제조된 베이스 액을 40 - 65℃와 20 - 30℃로 번갈아 열처리하여 유해균이 살균된 배양액을 제조하는 단계; 상기 제조된 배양액에 식물 성장에 필요한 양분을 공급하고 골고루 혼합하여 영양액을 제조하는 단계; 상기 제조된 영양액을 물과 혼합하여 식물 뿌리에 직접 공급할 수 있는 영양수용액을 제조하는 단계; 로 이루어진다.The present invention relates to a method for producing a nutrient solution for plant cultivation using a microbial culture medium that allows healthy cultivation of various flowers or fruit and vegetable plants using microorganisms through hydroponic cultivation. The composition consists of water, microorganisms necessary for plant growth, and microorganisms. Preparing a base solution by mixing the media necessary for growth; Preparing a culture solution in which harmful bacteria have been sterilized by alternately heat treating the prepared base solution at 40 - 65°C and 20 - 30°C; supplying nutrients necessary for plant growth to the prepared culture solution and mixing them evenly to prepare a nutrient solution; Mixing the prepared nutrient solution with water to prepare an aqueous nutrient solution that can be directly supplied to plant roots; It consists of
Description
본 발명은 미생물을 이용하여 화훼 또는 과채류 식물을 노지 또는 수경재배 방식으로 건강하게 재배할 수 있도록 한 미생물을 이용한 식물재배용 영양액 제조방법에 관한 것이다.The present invention relates to a method for producing a nutrient solution for plant cultivation using microorganisms, which allows healthy cultivation of flowers or fruit and vegetable plants in open field or hydroponic cultivation using microorganisms.
산업의 발전으로 식물의 성장에 있어, 성장 촉진 및 다양한 건강증진 성분 (예를들면, 미네랄, 특정 생약 유기물 등)을 포함한 기능성 식물의 제조를 원하고 있다. 이러한 시도들은 식물에 독성의 농약 대신 항균 기능을 갖는 금속 미네랄 투여, 특정 미네랄 함유 식물 재배, 식물의 성장촉진, 특정 기능성 유기물 생성, 식물을 통한 필수 영양소 함유 식물을 최종적으로 얻기 위하여 다양한 방법들이 개발되어 시행되고 있다.With the development of industry, there is a desire to manufacture functional plants containing growth-promoting and various health-promoting ingredients (e.g., minerals, specific herbal organic substances, etc.) in the growth of plants. These attempts include administering metal minerals with antibacterial properties instead of toxic pesticides to plants, cultivating plants containing specific minerals, promoting plant growth, producing specific functional organic substances, and various methods have been developed to ultimately obtain plants containing essential nutrients through plants. It is being implemented.
한편, 수경재배를 통한 친환경적 농작물의 생산기술 보급이 최근에 우리나라에서도 크게 확대되고 있다.Meanwhile, the spread of eco-friendly crop production technology through hydroponic cultivation has recently been expanding significantly in Korea.
특히, 정부(농촌진흥청)에서는 '청정인삼 고속생산 기술'을 수경재배기술로 재배방식으로 고속생산으로 무농약 청정재배가 가능하면서도 2년근 수삼을 불과 4개월 만에 생산하는 등 사업성이 매우 높다고 보도된 바 있다.In particular, the government (Rural Development Administration) reported that 'clean ginseng high-speed production technology' is a cultivation method using hydroponic cultivation technology, which enables pesticide-free clean cultivation through high-speed production and has very high business feasibility, such as producing 2-year-old fresh ginseng in just 4 months. There is a bar.
또한, 영양액을 이용한 수경재배방법은 작물(또는 식물)의 생육에 요구되는 필수원소를 그 흡수 비율에 따라 적당한 농도로 용해시킨 영양액을 이용하여 작물을 재배하는 방법으로서, 작물이 최적 생육조건에서 성장할 수 있도록 영양액과 배지 조건을 인위적으로 정밀하게 조절하여 작물을 재배하는 과학적인 영농기술이다.In addition, the hydroponic cultivation method using a nutrient solution is a method of cultivating crops using a nutrient solution in which essential elements required for the growth of crops (or plants) are dissolved at an appropriate concentration according to the absorption rate, and the crops will grow under optimal growth conditions. It is a scientific farming technology that cultivates crops by artificially and precisely controlling the nutrient solution and medium conditions to ensure optimal growth.
이와 같은 수경재배방법은 채소와 같은 소형 작물뿐만 아니라 화훼 작물 및 과수 등의 대형 작물의 재배에도 사용되고 있다.This hydroponic cultivation method is used for the cultivation of not only small crops such as vegetables, but also large crops such as flower crops and fruit trees.
이러한 수경재배는 작물이 심어진 재배용기와, 재배용기에 영양액을 지속적으로 공급하는 공급장치를 구비하여 이루어진다. 또한, 수경재배는 작물이 영양액을 흡수하여 성장이 원활하도록 환경을 조성하는 조명장치와 공조장치, 그리고 영양액을 살균하고 소독하는 부대설비가 부가적으로 구비되어 이루어진다.Such hydroponic cultivation is accomplished by providing a cultivation container in which crops are planted and a supply device that continuously supplies nutrient solution to the cultivation container. In addition, hydroponic cultivation is additionally equipped with lighting and air conditioning devices that create an environment for crops to absorb nutrient solution and grow smoothly, and auxiliary equipment to sterilize and disinfect the nutrient solution.
수경재배에 있어 일사량, 온도 및 습도 등은 기본적으로 중요한 요소이지만 사용되는 물은 대부분 해당 지역의 지하수를 사용함으로써, 물에 함유된 성분도 전기전도도(또는 총 용해성 고형물질), pH, 탁도, 미생물, 기체 류 및 경도성분 등에서 차이를 보이고 있으며, 영양액을 조성함에 있어 물에 따라 염 등을 일으키고, 농도에 지대한 영향을 준다.Solar radiation, temperature, and humidity are fundamentally important factors in hydroponic cultivation, but most of the water used is groundwater in the area, so the components contained in the water also include electrical conductivity (or total soluble solids), pH, turbidity, microorganisms, etc. There are differences in gas flow and hardness components, and when forming a nutrient solution, salts, etc. are generated depending on the water, which greatly affects the concentration.
여기서, 수경재배에 이용되는 영양액은 작물 각각의 표준적 생육을 도와주는 영양소와 영양액의 EC(Electrical Conductivity)가 일정하게 유지되도록 제조되었다. 또한, 이 영양액은 질소와 수용성 인산, 수용성 가리, 수용성 고토, 수용성 칼슘을 일정 비율로 혼합하고, 미량 원소를 첨가하여 제조되었다.Here, the nutrient solution used in hydroponic cultivation is manufactured so that the nutrients that help the standard growth of each crop and the EC (Electrical Conductivity) of the nutrient solution are kept constant. In addition, this nutrient solution was prepared by mixing nitrogen, water-soluble phosphoric acid, water-soluble potassium, water-soluble kaolin, and water-soluble calcium in a certain ratio, and adding trace elements.
그러나 이러한 종래의 수경재배용 영양액은 작물 발육에 적합하지 않고, 성장에 필요한 요소인 질소의 함량이 매우 높거나 낮아 견실한 작물 재배가 어렵다는 문제점이 있었다.However, these conventional nutrient solutions for hydroponic cultivation are not suitable for crop development, and the content of nitrogen, an element necessary for growth, is very high or low, making it difficult to grow crops.
본 발명은 상기 종래 문제점을 해소하고 화훼 또는 과채류 작물에 모두 적용하여 친환경으로 재배함과 동시에 상기 화훼 또는 과채류 식물이 건강하게 자랄 수 있도록 한 미생물을 이용한 식물재배용 영양액 제조방법을 제공함에 그 목적이 있다.The purpose of the present invention is to provide a method for producing a nutrient solution for plant cultivation using microorganisms that solves the above-mentioned problems and can be applied to all flower or fruit and vegetable crops to grow them in an eco-friendly manner and at the same time allows the flower or fruit and vegetable plants to grow healthily. .
상기 목적을 달성하기 위한 본 발명의 과제 해결 수단 구성은,
물 500 - 1500ℓ, 식물성장에 필요한 바실러스 벨레젠시스(Bacillus velezensis), 바실러스 서브틸리스(Bacillus Subtilis), 바실러스 아밀롤리퀘파시엔스(Bacillus Amyloliquefaciens), 바실러스 시아멘시즈(Bacillus Siamensis), 바실러스 리체니포르미스(Bacillus Licheniformis), 바실러스 퍼밀루스(Bacillus pumilus) 중 어느 하나 또는 2가지 이상이 혼합된 미생물 1.0×106-108 cfu/g 500 - 1500g, 미생물이 먹고 성장하는데 필요한 질소(N) 15 - 25중량%, 인(P) 15 - 25중량%, 칼륨 15 - 25중량%, 부형제 25 - 55중량%의 비율로 구성된 배지 2 - 6kg을 혼합하여 베이스 액을 제조하는 단계(S1);
상기 제조된 베이스 액을 40 - 65℃에서 6 - 24시간 열처리 후 20 - 30℃에서 18 - 72시간 동안 유지시키는 것을 적어도 1회 이상 실시로 번갈아 열처리하여 유해균이 살균된 배양액을 제조하는 단계(S2);
상기 제조된 배양액 500 - 1500ℓ에 식물 성장에 필요한 N(질소), P(인), K(칼륨), Ca(칼슘), Mg(마그네슘), S(황), Cu(구리), Fe(철), Zn(아연), Mo(몰리브덴), B(붕소), Mn(망간), Cl(염소), Ni(니켈), Co(코발트) 중 적어도 2종 이상이 혼합된 양분 3 - 15kg을 공급하고 골고루 혼합하여 영양액을 제조하는 단계(S3);The configuration of the problem-solving means of the present invention to achieve the above purpose is,
500 - 1500 liters of water, required for plant growth: Bacillus velezensis, Bacillus Subtilis, Bacillus Amyloliquefaciens, Bacillus Siamensis, Bacillus licheni Microorganisms containing one or two or more of Bacillus Licheniformis and Bacillus pumilus 1.0×10 6 -10 8 cfu/g 500 - 1500g, nitrogen (N) required for microorganisms to eat and grow 15 - Preparing a base liquid by mixing 2 to 6 kg of medium consisting of 25% by weight, 15 to 25% by weight of phosphorus (P), 15 to 25% by weight of potassium, and 25 to 55% by weight of excipients (S1);
Preparing a culture solution in which harmful bacteria have been sterilized by alternately heat treating the prepared base liquid at 40 - 65°C for 6 - 24 hours and then maintaining it at 20 - 30°C for 18 - 72 hours at least once (S2) );
500 - 1500 liters of the prepared culture medium contains N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium), S (sulfur), Cu (copper), and Fe (iron) necessary for plant growth. ), Zn (zinc), Mo (molybdenum), B (boron), Mn (manganese), Cl (chlorine), Ni (nickel), and Co (cobalt) mixed with 3 to 15 kg of nutrients. and mixing evenly to prepare a nutrient solution (S3);
상기 제조된 영양액을 물과 혼합하여 식물 뿌리에 직접 공급할 수 있는 560 - 3500 TDS(총 용존 고형량(total dissolved solid)를 유지하도록 물에 영양액을 공급하여 된 영양수용액을 제조하는 단계(S4); 로 이루어진다.The prepared nutrient solution is mixed with water to prepare a nutrient solution by supplying the nutrient solution to water to maintain 560 - 3500 TDS (total dissolved solid), which can be directly supplied to plant roots (S4); It comes true.
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상기와 같은 구성을 가지는 본 발명의 미생물을 이용한 식물재배용 영양액 제조방법은, 상기 목적에서 설명한 바와 같이 화훼 또는 과채류 식물에 모두 적용하여 친환경으로 재배함과 동시에 상기 화훼 또는 과채류 식물이 건강하게 자랄 수 있도록 하는 효과가 있다.The method for producing a nutrient solution for plant cultivation using the microorganism of the present invention having the above-described structure can be applied to all flowers or fruit and vegetable plants as described for the above purpose to cultivate them in an eco-friendly manner and at the same time allow the flowers or fruit and vegetable plants to grow healthily. There is an effect.
도 1은 본 발명의 식물재배용 영양액을 제조하고, 그 제조된 영양액을 노지 재배식물에 공급하는 과정을 개략적으로 나타낸 구성도,
도 2는 본 발명의 식물재배용 영양액을 제조하고, 그 제조된 영양액을 수경재배식물에 공급하는 과정을 개략적으로 나타낸 사진. 1 is a configuration diagram schematically showing the process of manufacturing a nutrient solution for plant cultivation of the present invention and supplying the prepared nutrient solution to field-grown plants;
Figure 2 is a photograph schematically showing the process of preparing the nutrient solution for plant cultivation of the present invention and supplying the prepared nutrient solution to hydroponic plants.
본 발명은 미생물을 이용하여 화훼 또는 과채류 식물을 노지 또는 수경재배 방식으로 건강하게 재배할 수 있도록 한 미생물을 이용한 식물재배용 영양액 제조방법에 관한 것으로, 이를 실시 예를 들어 설명하면 아래와 같다.The present invention relates to a method for producing a nutrient solution for plant cultivation using microorganisms, which allows healthy cultivation of flowers or fruit and vegetable plants in open field or hydroponic cultivation using microorganisms. This is explained with an example as follows.
- 아 래 -- under -
본 발명의 미생물을 이용한 식물재배용 영양액 제조방법은,
물 500 - 1500ℓ, 식물성장에 필요한 바실러스 벨레젠시스(Bacillus velezensis), 바실러스 서브틸리스(Bacillus Subtilis), 바실러스 아밀롤리퀘파시엔스(Bacillus Amyloliquefaciens), 바실러스 시아멘시즈(Bacillus Siamensis), 바실러스 리체니포르미스(Bacillus Licheniformis), 바실러스 퍼밀루스(Bacillus pumilus) 중 어느 하나 또는 2가지 이상이 혼합된 미생물 1.0×106-108 cfu/g 500 - 1500g, 미생물이 먹고 성장하는데 필요한 질소(N) 15 - 25중량%, 인(P) 15 - 25중량%, 칼륨 15 - 25중량%, 부형제 25 - 55중량%의 비율로 구성된 배지 2 - 6kg을 혼합하여 베이스 액을 제조하는 단계(S1);
상기 제조된 베이스 액을 40 - 65℃에서 6 - 24시간 열처리 후 20 - 30℃에서 18 - 72시간 동안 유지시키는 것을 적어도 1회 이상 실시로 번갈아 열처리하여 유해균이 살균된 배양액을 제조하는 단계(S2);
상기 제조된 배양액 500 - 1500ℓ에 식물 성장에 필요한 N(질소), P(인), K(칼륨), Ca(칼슘), Mg(마그네슘), S(황), Cu(구리), Fe(철), Zn(아연), Mo(몰리브덴), B(붕소), Mn(망간), Cl(염소), Ni(니켈), Co(코발트) 중 적어도 2종 이상이 혼합된 양분 3 - 15kg을 공급하고 골고루 혼합하여 영양액을 제조하는 단계(S3);The method for producing a nutrient solution for plant cultivation using microorganisms of the present invention,
500 - 1500 liters of water, required for plant growth: Bacillus velezensis, Bacillus Subtilis, Bacillus Amyloliquefaciens, Bacillus Siamensis, Bacillus licheni Microorganisms containing one or two or more of Bacillus Licheniformis and Bacillus pumilus 1.0×10 6 -10 8 cfu/g 500 - 1500g, nitrogen (N) required for microorganisms to eat and grow 15 - Preparing a base liquid by mixing 2 to 6 kg of medium consisting of 25% by weight, 15 to 25% by weight of phosphorus (P), 15 to 25% by weight of potassium, and 25 to 55% by weight of excipients (S1);
Preparing a culture solution in which harmful bacteria have been sterilized by alternately heat treating the prepared base liquid at 40 - 65°C for 6 - 24 hours and then maintaining it at 20 - 30°C for 18 - 72 hours at least once (S2) );
In 500 - 1500 liters of the culture medium prepared above, N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium), S (sulfur), Cu (copper), and Fe (iron) necessary for plant growth. ), Zn (zinc), Mo (molybdenum), B (boron), Mn (manganese), Cl (chlorine), Ni (nickel), and Co (cobalt) mixed with 3 to 15 kg of nutrients. and mixing evenly to prepare a nutrient solution (S3);
상기 제조된 영양액을 물과 혼합하여 식물 뿌리에 직접 공급할 수 있는 560 - 3500 TDS(총 용존 고형량(total dissolved solid)를 유지하도록 물에 영양액을 공급하여 된 영양수용액을 제조하는 단계(S4); 로 이루어진다.The prepared nutrient solution is mixed with water to prepare a nutrient solution by supplying the nutrient solution to water to maintain 560 - 3500 TDS (total dissolved solid), which can be directly supplied to plant roots (S4); It comes true.
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상기와 같은 구성의 베이스 액을 제조하는 (S1)단계에서 물, 미생물, 배지의 혼합 비율 예는, 물 500 - 1500ℓ, 미생물 1.0×106-108 cfu/g 500 - 1500g, 배지 2 - 6kg로 함이 바람직하다.An example of the mixing ratio of water, microorganisms, and medium in the (S1) step of preparing the base liquid with the above composition is 500 - 1500 liters of water, 1.0 × 10 6 -10 8 cfu/g of microorganisms, 500 - 1500 g of medium, 2 - 6 kg of medium. It is desirable to do so.
상기 물 500 - 1500ℓ에 미생물 1.0×106-108 cfu/g 500 - 1500g을 혼합하는 이유는, 물속에서 미생물이 활발하게 활동하며 배양할 수 있도록 하기 위함이고, 상기 배지 2 - 6kg을 공급하는 이유는, 상기 미생물이 활발하게 활동하며 배양할 때 충분한 먹이를 공급할 수 있도록 하기 위함이다.The reason for mixing 500 - 1500 g of microorganisms 1.0 The reason is to ensure that the microorganisms are active and provide sufficient food when cultured.
상기에서 설명한 미생물의 활발한 활동과 배양에 필요한 최적의 물, 미생물, 배지의 혼합비율은, 물 1000ℓ, 미생물 1.0×106 cfu/g 1000g, 배지 4kg이다.The optimal mixing ratio of water, microorganisms, and medium required for the active activity and cultivation of microorganisms described above is 1000 liters of water, 1.0 × 10 6 cfu/g 1000 g of microorganisms, and 4 kg of medium.
또한, 물에 혼합되는 미생물은, 바실러스 벨레젠시스(Bacillus velezensis), 바실러스 서브틸리스(Bacillus Subtilis), 바실러스 아밀롤리퀘파시엔스(Bacillus Amyloliquefaciens), 바실러스 시아멘시즈(Bacillus Siamensis), 바실러스 리체니포르미스(Bacillus Licheniformis), 바실러스 퍼밀루스(Bacillus pumilus) 중 어느 하나 또는 2가지 이상이 혼합된 것을 사용할 수 있다.In addition, microorganisms mixed in water include Bacillus velezensis, Bacillus Subtilis, Bacillus Amyloliquefaciens, Bacillus Siamensis, and Bacillus licheni. Any one of Bacillus Licheniformis and Bacillus pumilus or a mixture of two or more can be used.
상기의 각 미생물에서 바실러스(Bacillus)는, 식물의 생육증진과 면역력 증강뿐만 아리라 식물 병에 대한 저항성을 증대시키는 것으로 알려지면서 식물 프로바이오틱스 중에 가장 많이 연구 사용되고 있다.Among the above-mentioned microorganisms, Bacillus is known to not only promote plant growth and enhance immunity, but also increase resistance to plant diseases, and is the most studied and used plant probiotic.
상기의 미생물에서In the above microorganisms
1) 바실러스 벨레젠시스(Bacillus velezensis)와 바실러스 아밀롤리퀘파시엔스(Bacillus Amyloliquefaciens)는, 식물 생육을 튼튼하게 하고 면역력을 증대시키며 병 방제 효과가 있고,1) Bacillus velezensis and Bacillus Amyloliquefaciens strengthen plant growth, increase immunity, and have a disease control effect.
2) 상기 바실러스 서브틸리스(Bacillus Subtilis)는, 땅속의 유기물 발효에 효과가 있으며,2) The Bacillus Subtilis is effective in fermenting organic matter in the ground,
3) 상기 바실러스 시아멘시즈(Bacillus Siamensis)는, 오염된 토양을 복구하는데 효과가 있고,3) The Bacillus Siamensis is effective in restoring contaminated soil,
4) 상기 바실러스 리체니포르미스(Bacillus Licheniformis)는, 단백질 성분으로 오염된 것을 복구하고, 퇴비 부숙 효과가 있으며,4) The Bacillus Licheniformis restores contamination with protein components and has a compost composting effect,
5) 상기 바실러스 퍼밀루스(Bacillus pumilus)는, 방제용 농약으로 이용하는 토양 미생물로써, 친환경적으로 병충해를 방제할 수 있는 효과가 있다.5) The Bacillus pumilus is a soil microorganism used as a pesticide for pest control, and is effective in controlling pests in an environmentally friendly manner.
상기에서 설명한 바와 같이 베이스 액을 제조하는 (S1)단계에서 물에 미생물을 혼합하는 이유는, 상기 1) 내지 5)의 효과를 누리기 위함이다.As explained above, the reason for mixing microorganisms with water in step (S1) of preparing the base liquid is to enjoy the effects of 1) to 5) above.
또한, 상기 베이스 액을 제조하는 (S1)단계에서, 배지는, 질소(N) 15 - 25중량%, 인(P) 15 - 25중량%, 칼륨 15 - 25중량%, 부형제 25 - 55중량%의 비율로 구성됨이 바람직한데 그 이유는, 상기의 질소, 인, 칼륨의 비율이 잘못되어 어느 한 성분의 비율이 높고, 다른 성분의 비율이 낮을 경우 미생물의 먹이로 부적합하기 때문에 상기 배지의 성분은 질소(N) 15 - 25중량%, 인(P) 15 - 25중량%, 칼륨 15 - 25중량%, 부형제 25 - 55중량%의 비율로 함이 바람직하다.In addition, in the step (S1) of preparing the base liquid, the medium contains 15 - 25% by weight of nitrogen (N), 15 - 25% by weight of phosphorus (P), 15 - 25% by weight of potassium, and 25 - 55% by weight of excipients. It is preferable that the ratio of nitrogen, phosphorus, and potassium is incorrect, and if the ratio of one component is high and the ratio of the other component is low, it is unsuitable as food for microorganisms, so the components of the medium are nitrogen. (N) 15 - 25% by weight, phosphorus (P) 15 - 25% by weight, potassium 15 - 25% by weight, and excipients 25 - 55% by weight.
상기 제조된 베이스 액을 이용하여 배양액을 제조하는 (S2)단계는, 상기 제조된 베이스 액을 40 - 65℃와 20 - 30℃로 번갈아 열처리하여 베이스 액에 존재하는 유해균을 살균하고 또 미생물 배양을 유도하는 것으로, 상기 열처리의 실시 예는, 베이스 액을 40 - 65℃와 20 - 30℃로 1 - 7회 실시함이 바람직하다.In the step (S2) of preparing a culture solution using the prepared base solution, the prepared base solution is alternately heat treated at 40 - 65 ℃ and 20 - 30 ℃ to sterilize harmful bacteria present in the base solution and to cultivate microorganisms. In conclusion, in the above heat treatment example, it is preferable to perform the base liquid 1 to 7 times at 40 - 65°C and 20 - 30°C.
상기 베이스 액을 40 - 65℃로 열처리하는 이유는, 베이스 액에 존재하는 유해균을 살균하고자 하는 것이고, 20 - 30℃를 유지하는 이유는, 미생물의 배양을 촉진시키기 위함이다.The reason for heat treating the base liquid at 40 - 65°C is to sterilize harmful bacteria present in the base liquid, and the reason for maintaining the temperature at 20 - 30°C is to promote the culture of microorganisms.
그리고 상기 40 - 65℃로 열처리시간은 6 - 24시간 함이 바람직한데, 6시간 미만하면 유해균이 살균이 미미하고, 24시간을 초과하면 유익한 미생물 배양에 지장을 초래할 수 있음으로 40 - 65℃로 열처리하는 시간은 6 - 24시간으로 함이 바람직하다.In addition, it is preferable that the heat treatment time at 40 - 65 ℃ is 6 - 24 hours. If it is less than 6 hours, the sterilization of harmful bacteria is minimal, and if it exceeds 24 hours, it may interfere with the cultivation of beneficial microorganisms. The heat treatment time is preferably 6 to 24 hours.
또 20 - 30℃를 18 - 72시간 동안 유지시키는 이유는, 미생물의 배양이 충분히 일어나도록 하기 위함이다.Also, the reason for maintaining 20 - 30℃ for 18 - 72 hours is to ensure sufficient cultivation of microorganisms.
상기와 같은 이유의 베이스 액 열처리 횟수는 3회 이상 함이 바람직한데, 그 이유는, 미생물 배양이 충분하게 이루어지도록 하기 위함이고, 그 미생물 배양 과정에서 유해균이 발생함으로 그 유해균을 40 - 65℃에서 6 - 24시간 열처리하는 것이다.For the above reasons, it is desirable to heat treat the base liquid three times or more. The reason is to ensure sufficient microbial culture. As harmful bacteria are generated during the microbial culture process, the harmful bacteria must be treated at 40 - 65°C. It is heat treated for 6 to 24 hours.
본 발명의 상기 제조된 배양액을 이용하여 영양액을 제조하는 (S3)단계는, 식물 성장에 필요한 양분을 공급하고 골고루 혼합하여 영양액을 제조하는 것으로, 상기 양분 공급의 실시 예는, N(질소), P(인), K(칼륨)이 동일한 양으로 혼합하거나 다른 실시 예로, N(질소), P(인), K(칼륨), Ca(칼슘), Mg(마그네슘), S(황), Cu(구리), Fe(철), Zn(아연), Mo(몰리브덴), B(붕소), Mn(망간), Cl(염소), Ni(니켈), Co(코발트) 중 적어도 2종 이상을 공급하는 것이다.The (S3) step of preparing a nutrient solution using the prepared culture solution of the present invention is to supply nutrients necessary for plant growth and mix them evenly to prepare a nutrient solution. Examples of the nutrient supply include N (nitrogen), P (phosphorus) and K (potassium) are mixed in equal amounts, or in another embodiment, N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium), S (sulfur), Cu Supply at least two of (copper), Fe (iron), Zn (zinc), Mo (molybdenum), B (boron), Mn (manganese), Cl (chlorine), Ni (nickel), and Co (cobalt). It is done.
상기의 양분은 식물이 건강하게 생육함에 있어 꼭 필요한 성분이다.The above nutrients are essential for healthy growth of plants.
상기와 같은 영양액을 제조하는 (S3)단계에서, 배양액과 양분의 혼합비는 배양액 500 - 1500ℓ, 양분 3 - 15kg로 함이 바람직하데, 그 이유는, 식물의 노지 또는 수경재배 방식에 따라 다르고, 또 식물의 종류에 따라 다르기 때문에 상기 재배하는 곳이 노지인지 또는 수경인지와 재배식물이 어느 식물인지에 따라 상기 양분 3 - 15kg를 적정하게 혼합한다. In the step (S3) of preparing the nutrient solution as described above, the mixing ratio of the culture solution and nutrients is preferably 500 - 1,500 liters of culture solution and 3 - 15 kg of nutrients. The reason is that it varies depending on the open field or hydroponic cultivation method of the plant, and Since it varies depending on the type of plant, 3 to 15 kg of the above nutrients are appropriately mixed depending on whether the cultivation area is open field or hydroponic and which plant is being cultivated.
본 발명의 상기 제조된 영양액을 물과 혼합하여 식물 뿌리에 직접 공급할 수 있는 영양수용액을 제조하는 (S4)단계는, 영양수용액이 TDS(총 용존 고형량(total dissolved solid) 560 - 3500 유지하도록 물에 영양약을 공급하는 것으로, 상기 TDS범위를 560 - 3500로 하는 이유는, 아래 표 1에 나타낸 바와 같이 재배식물에 따라 적정 TDS가 다르기 때문이다.In the step (S4) of preparing a nutrient solution that can be directly supplied to plant roots by mixing the prepared nutrient solution of the present invention with water, the nutrient solution is added to water to maintain TDS (total dissolved solids) of 560 - 3500. The reason why the TDS range is 560 - 3500 when supplying nutrients is because the appropriate TDS varies depending on the cultivated plant, as shown in Table 1 below.
[표 1][Table 1]
이상에서 설명한 바와 같은 본 발명의 제조방법으로 제조된 미생물을 이용한 식물재배용 영양액은 상기 표 1에서 나타낸 바와 같이 화훼 또는 과채류 식물에 모두 적용하여 친환경으로 재배함과 동시에 상기 화훼 또는 과채류 식물이 건강하게 자랄 수 있도록 하는 장점이 있다.The nutrient solution for plant cultivation using microorganisms prepared by the manufacturing method of the present invention as described above can be applied to all flower or fruit and vegetable plants as shown in Table 1 above to cultivate them in an eco-friendly manner and at the same time help the flower or fruit and vegetable plants grow healthily. There is an advantage in allowing this.
Claims (9)
상기 제조된 베이스 액을 40 - 65℃에서 6 - 24시간 열처리 후 20 - 30℃에서 18 - 72시간 동안 유지시키는 것을 적어도 1회 이상 실시로 번갈아 열처리하여 유해균이 살균된 배양액을 제조하는 단계(S2);
상기 제조된 배양액 500 - 1500ℓ에 식물 성장에 필요한 N(질소), P(인), K(칼륨), Ca(칼슘), Mg(마그네슘), S(황), Cu(구리), Fe(철), Zn(아연), Mo(몰리브덴), B(붕소), Mn(망간), Cl(염소), Ni(니켈), Co(코발트) 중 적어도 2종 이상이 혼합된 양분 3 - 15kg을 공급하고 골고루 혼합하여 영양액을 제조하는 단계(S3);
상기 제조된 영양액을 물과 혼합하여 식물 뿌리에 직접 공급할 수 있는 560 - 3500 TDS(총 용존 고형량(total dissolved solid)를 유지하도록 물에 영양액을 공급하여 된 영양수용액을 제조하는 단계(S4); 로 이루어진 미생물을 이용한 식물재배용 영양액 제조방법.500 - 1500 liters of water, required for plant growth: Bacillus velezensis, Bacillus Subtilis, Bacillus Amyloliquefaciens, Bacillus Siamensis, Bacillus licheni Microorganisms containing one or two or more of Bacillus Licheniformis and Bacillus pumilus 1.0×10 6 -10 8 cfu/g 500 - 1500g, nitrogen (N) required for microorganisms to eat and grow 15 - Preparing a base liquid by mixing 2 to 6 kg of medium consisting of 25% by weight, 15 to 25% by weight of phosphorus (P), 15 to 25% by weight of potassium, and 25 to 55% by weight of excipients (S1);
Preparing a culture solution in which harmful bacteria have been sterilized by alternately heat treating the prepared base liquid at 40 - 65°C for 6 - 24 hours and then maintaining it at 20 - 30°C for 18 - 72 hours at least once (S2) );
In 500 - 1500 liters of the culture medium prepared above, N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium), S (sulfur), Cu (copper), and Fe (iron) necessary for plant growth. ), Zn (zinc), Mo (molybdenum), B (boron), Mn (manganese), Cl (chlorine), Ni (nickel), and Co (cobalt) mixed with 3 to 15 kg of nutrients. and mixing evenly to prepare a nutrient solution (S3);
The prepared nutrient solution is mixed with water to prepare a nutrient solution by supplying the nutrient solution to water to maintain 560 - 3500 TDS (total dissolved solid), which can be directly supplied to plant roots (S4); Method for producing nutrient solution for plant cultivation using microorganisms.
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