KR102283528B1 - Cosmetic composition comprising cereal and seaweed mixed fermented extract using Enterococcus faecium MSS2 capable of fermenting at room temperature - Google Patents
Cosmetic composition comprising cereal and seaweed mixed fermented extract using Enterococcus faecium MSS2 capable of fermenting at room temperature Download PDFInfo
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- KR102283528B1 KR102283528B1 KR1020190009873A KR20190009873A KR102283528B1 KR 102283528 B1 KR102283528 B1 KR 102283528B1 KR 1020190009873 A KR1020190009873 A KR 1020190009873A KR 20190009873 A KR20190009873 A KR 20190009873A KR 102283528 B1 KR102283528 B1 KR 102283528B1
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- KR
- South Korea
- Prior art keywords
- fermented
- mixed
- extract
- red bean
- enterococcus faecium
- Prior art date
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Abstract
본 발명은 상온발효 가능한 엔테로코쿠스 파에시움 MSS2(Enterococcus faecium MSS2) 유산균을 이용한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 포함하는 화장료 조성물에 대한 것으로, 본 발명에 따른 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물 조성물은 항산화, 주름개선, 미백 등 다양한 효과를 나타내어 기능성 화장품 또는 건강기능식품 소재로서 유용하게 활용될 수 있다.The present invention relates to a cosmetic composition comprising a grain and seaweed mixed fermented extract using Enterococcus faecium MSS2 (Enterococcus faecium MSS2) lactic acid bacteria that can be fermented at room temperature as an active ingredient, and Enterococcus faecium MSS2 according to the present invention The grain and seaweed mixed fermented extract composition fermented with fermented with fermented algae exhibits various effects such as antioxidant, wrinkle improvement, and whitening, and thus can be usefully used as a material for functional cosmetics or health functional food.
Description
본 발명은 상온발효 가능한 엔테로코쿠스 파에시움 MSS2(Enterococcus faecium MSS2) 유산균을 이용한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 포함하는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition comprising, as an active ingredient, a mixed fermented extract of grains and seaweeds using Enterococcus faecium MSS2 (Enterococcus faecium MSS2) lactic acid bacteria that can be fermented at room temperature.
최근 의학의 발달과 생활환경의 개선에 의해 인간의 수명이 크게 연장됨에 따라 노화에 대한 관심이 높아져 많은 노화 관련 연구가 이루어지고 있다. 그 중에서도 경제적, 사회적 여유가 생기게 되면서 외모에 대한 관심이 많아져 젊고 깨끗한 피부를 갖고 싶어 함에 따라 피부의 노화 현상을 지연 또는 방지할 수 있는 소재 개발에 많은 관심이 집중되고 있다.Recently, as the lifespan of human beings is greatly extended due to the development of medicine and the improvement of the living environment, interest in aging has increased, and many studies related to aging are being conducted. Among them, as economic and social leeway is created, interest in appearance increases, and as people want to have young and clean skin, much attention is being paid to the development of materials that can delay or prevent skin aging.
노화로 인해 피부의 구성 성분인 표피, 진피 및 피하조직의 두께가 얇아지고 피부에 탄력을 주는 세포외 기질(Extracellular matrix, ECM) 성분이 변화하게 되는데, 그중 세포외 기질(ECM)의 70~80%를 차지하는 콜라겐은 나이가 들면서 그 생성이 급격하게 저하되어 피부가 탄력을 잃고 주름이 과도하게 형성되면서 노인성 피부로 변화되어 간다. Due to aging, the thickness of the epidermis, dermis, and subcutaneous tissue, which are components of the skin, becomes thinner and the extracellular matrix (ECM) component that gives elasticity to the skin changes. Collagen, which occupies the %, rapidly decreases with age, and the skin loses elasticity and wrinkles are excessively formed, changing to senile skin.
나이가 들면서 피부 결합조직 내의 콜라겐 함량이 줄어드는 것은 콜라겐 합성의 저하와 분해의 촉진에 의한 것이다. 따라서 콜라겐 생합성의 저하와 콜라겐 분해 촉진은 피부 주름 형성의 가장 큰 원인이라 할 수 있다. 콜라겐 생합성 과정은 전사 수준(Transcription level)과 해독 후 수준(Post-translation level)에 관여하는 많은 인자(Factor)들에 의해 조절을 받아 변화를 일으키며, 콜라겐 분해는 자외선 등에 의해 콜라겐을 분해하는 콜라게네이즈(Collagenase)와 같은 기질금속단백질 분해 효소들(Matrix metalloproteases, MMP)의 발현의 촉진으로 콜라겐 분해가 촉진되어 콜라겐 함량이 줄어든다. 아울러 외부 환경에 의해 콜라겐의 변형이 가속화되면서 피부는 주름이 많아지고 깊어지게 된다. The decrease in collagen content in the skin connective tissue with age is due to the decrease in collagen synthesis and promotion of degradation. Therefore, the decrease in collagen biosynthesis and the promotion of collagen decomposition are the biggest causes of skin wrinkle formation. The collagen biosynthesis process is regulated by many factors involved in the transcription level and post-translation level, and the collagen breakdown is collagen that breaks down collagen by ultraviolet rays, etc. Collagen decomposition is promoted by promotion of expression of matrix metalloproteases (MMP) such as collagenase, thereby reducing collagen content. In addition, as the transformation of collagen is accelerated by the external environment, the skin becomes wrinkled and deep.
또한, 세포 내에서 티로시네이즈에 의해 타이로신으로부터 도파(dopa), 도파퀴논(dopaquinone)으로 변환되어 도파크롬(dopachrome) 등을 거처 생성되어지는 멜라닌은 피부에 존재하여 자외선 등으로부터 신체를 보호하는 중요한 기능을 가지고 있다. 그러나 멜라닌이 과잉 생산됨으로써 기미, 주근깨 등을 형성하고, 피부노화를 촉진하며, 피부암 유발에 중요한 작용을 하는 것으로 알려져 있으며, 또한 과실류나 채소류 등의 식품에서의 과잉 축적으로 인해 갈변화 현상을 일으키는 주요 원인으로 작용하기도 한다. In addition, melanin, which is converted from tyrosine to dopa and dopaquinone by tyrosinase in the cell and generated through dopachrome, is present in the skin and is important to protect the body from UV rays. has a function. However, due to excessive production of melanin, it is known to form spots and freckles, promote skin aging, and play an important role in inducing skin cancer. It can also act as a cause.
이에, 항산화, 주름개선 및 미백 효과를 갖는 기능성 화장품 소재 개발에 대한 관심이 높아졌으나, 아직까지 곡류 및 해조류를 첨가하고 특정 균주로 상온 발효한 곡류 및 해조류 혼합 발효 추출물의 기능성 화장품 소재에 대한 상용화는 부족한 실정이며, 곡류 및 해조류 혼합 발효 추출물을 이용한 기능성 화장품 소재 개발이 여전히 필요하다.Accordingly, interest in the development of functional cosmetic materials having antioxidant, wrinkle improvement and whitening effects has increased, but commercialization of functional cosmetic materials of grain and seaweed mixed fermented extracts added with grains and seaweed and fermented at room temperature with a specific strain has yet to be commercialized. The situation is insufficient, and there is still a need to develop functional cosmetic materials using a mixed fermented extract of cereals and seaweed.
상기와 같은 문제점을 해결하기 위해, 본 발명의 목적은 특정 균주로 발효한 곡류 및 해조류 혼합 발효 추출물을 이용한 기능성 화장료 조성물을 제공하는 데에 있다.In order to solve the above problems, an object of the present invention is to provide a functional cosmetic composition using a mixed fermented extract of grains and seaweeds fermented with a specific strain.
또한, 본 발명의 다른 목적은 특정 균주로 발효한 곡류 및 해조류 혼합 발효 추출물을 이용한 건강기능식품 조성물을 제공하는 데에 있다.Another object of the present invention is to provide a health functional food composition using a mixed fermented extract of grains and seaweeds fermented with a specific strain.
또한, 본 발명의 또 다른 목적은 특정 균주로 발효한 곡류 및 해조류 혼합 발효 추출물을 제조하는 방법을 제공하는 데에 있다.Another object of the present invention is to provide a method for preparing a mixed fermented extract of grains and seaweeds fermented with a specific strain.
본 발명은 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 함유하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition comprising, as an active ingredient, a mixed fermented extract of grains and seaweeds fermented with Enterococcus faecium MSS2.
또한, 본 발명은 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 함유하는 건강기능식품 조성물을 제공한다.In addition, the present invention provides a health functional food composition containing, as an active ingredient, a mixed fermented extract of grains and seaweeds fermented with Enterococcus faecium MSS2.
또한, 본 발명은 (1) 자연 건조한 곡류 및 해조류 혼합 분말에 물을 첨가하고 100~130℃에서 추출하는 단계; (2) 상기 추출된 추출물에 엔테로코쿠스 파에시움 MSS2을 접종하고 상온 발효하는 단계; 및 (3) 상기 발효된 발효물을 원심분리 하여 상층액을 회수하고 필터로 여과하여 균체와 곡류 및 해조류 분말을 제거한 발효물을 제조하는 단계를 포함하는, 곡류 및 해조류 혼합 발효 추출물의 제조방법을 제공한다.In addition, the present invention comprises the steps of (1) adding water to a naturally dried grain and seaweed mixed powder and extracting it at 100 ~ 130 ℃; (2) inoculating the extracted extract with Enterococcus faecium MSS2 and fermenting at room temperature; and (3) centrifuging the fermented fermented product to recover the supernatant and filtering it through a filter to prepare a fermented product from which cells and grains and seaweed powder are removed. to provide.
본 발명은 상온발효 가능한 엔테로코쿠스 파에시움 MSS2(Enterococcus faecium MSS2) 유산균을 이용한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 포함하는 화장료 조성물에 대한 것으로, 본 발명에 따른 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물 조성물은 항산화, 주름개선, 미백 등 다양한 효과를 나타내어 기능성 화장품 또는 건강기능식품 소재로서 유용하게 활용될 수 있다.The present invention relates to a cosmetic composition comprising a grain and seaweed mixed fermented extract using Enterococcus faecium MSS2 (Enterococcus faecium MSS2) lactic acid bacteria that can be fermented at room temperature as an active ingredient, and Enterococcus faecium MSS2 according to the present invention The grain and seaweed mixed fermented extract composition fermented with fermented with fermented algae exhibits various effects such as antioxidant, wrinkle improvement, and whitening, and thus can be usefully used as a material for functional cosmetics or health functional food.
도 1은 본 발명에 따른 혼합 발효 추출물의 제조공정을 나타낸다.
도 2는 본 발명에 따른 혼합 발효 추출물의 발효 시간별 pH 변화 측정 결과를 나타낸다.
도 3은 본 발명에 따른 혼합 발효 추출물의 총 페놀 함량 분석 결과를 나타낸다.
도 4는 본 발명에 따른 혼합 발효 추출물의 총 플라보노이드 함량 분석 결과를 나타낸다.
도 5는 본 발명에 따른 혼합 발효 추출물의 DPPH 라디칼 소거 효과 분석 결과를 나타낸다.
도 6은 본 발명에 따른 혼합 발효 추출물의 티로시네이즈 억제 효과 분석 결과를 나타낸다.
도 7은 본 발명에 따른 혼합 발효 추출물의 콜라게네이즈 억제 효과 분석 결과를 나타낸다.
도 8은 본 발명에 따른 혼합 발효 추출물의 엘라스테이즈 억제 효과 분석 결과를 나타낸다.
도 9는 본 발명에 따른 혼합 발효 추출물의 멜라노마 세포 생존률 분석 결과를 나타낸다.
도 10은 본 발명에 따른 혼합 발효 추출물의 섬유아세포 생존률 분석 결과를 나타낸다.
도 11은 본 발명에 따른 혼합 발효 추출물의 멜라닌 함량 분석 결과를 나타낸다.
도 12는 본 발명에 따른 혼합 발효 추출물의 세포 내 티로시네이즈 활성 분석 결과를 나타낸다.1 shows a manufacturing process of a mixed fermented extract according to the present invention.
2 shows the measurement result of pH change according to fermentation time of the mixed fermented extract according to the present invention.
3 shows the analysis result of the total phenol content of the mixed fermented extract according to the present invention.
4 shows the results of analysis of the total flavonoid content of the mixed fermented extract according to the present invention.
5 shows the analysis results of the DPPH radical scavenging effect of the mixed ferment extract according to the present invention.
6 shows the results of analysis of the tyrosinase inhibitory effect of the mixed fermented extract according to the present invention.
7 shows the results of analysis of the collagenase inhibitory effect of the mixed fermented extract according to the present invention.
8 shows the results of analysis of the elastase inhibitory effect of the mixed fermented extract according to the present invention.
9 shows the results of analysis of melanoma cell viability of the mixed ferment extract according to the present invention.
10 shows the fibroblast viability analysis results of the mixed fermented extract according to the present invention.
11 shows the results of analysis of the melanin content of the mixed fermented extract according to the present invention.
12 shows the results of analysis of intracellular tyrosinase activity of the mixed ferment extract according to the present invention.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명자들은 곡류 및 해조류를 이용한 기능성 화장품 소재 개발을 위해 연구 노력한 결과, 팥과 같은 곡류 및 톳과 같은 해조류에 상온 발효가 가능한 특정 균주인 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물이 탁월한 항산화, 미백 및 주름개선 효과를 나타내는 것을 확인함으로써 본 발명을 완성하였다.As a result of research efforts to develop functional cosmetic materials using grains and seaweeds, the present inventors have fermented grains and seaweeds with Enterococcus faecium MSS2, a specific strain capable of fermenting grains such as red beans and seaweeds at room temperature at room temperature. The present invention was completed by confirming that the extract exhibits excellent antioxidant, whitening and anti-wrinkle effects.
본 발명에서 "추출물"은 추출 방법, 추출 용매, 추출된 성분 또는 추출물의 형태를 불문하고, 천연물의 성분을 뽑아냄으로써 얻어진 물질을 모두 포함하는 것이며, 또한 천연물의 성분을 뽑아내어 얻어진 물질을 추출 후 다른 방법으로 가공 또는 처리하여 얻어질 수 있는 물질을 모두 포함하는 광범위한 개념이다.In the present invention, "extract" includes all substances obtained by extracting the components of a natural product, regardless of the extraction method, extraction solvent, extracted component, or the form of the extract, and also after extracting the material obtained by extracting the component of the natural product It is a broad concept encompassing all substances that can be obtained by processing or processing in other ways.
본 발명에서 "화장료"는 피부 노화 등의 예방 또는 개선의 효과를 가지는 기능성 화장료를 포함할 수 있고, 상기 기능성 화장료는 화장품법에 정의된 바에 따라 특정 효능과 효과가 강조된 화장료로 피부의 주름 개선에 도움을 주거나 피부의 기능 약화로 인한 건조함을 방지하는 것 등을 포함할 수 있다.In the present invention, "cosmetics" may include functional cosmetics having an effect of preventing or improving skin aging, etc., and the functional cosmetics are cosmetics emphasizing specific efficacy and effects as defined in the Cosmetics Act, helping to improve wrinkles on the skin It may include giving or preventing dryness due to weakening of the skin's function.
본 발명에서 "건강기능식품"은 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미한다. 여기서 '기능성'이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻는 것을 의미한다. In the present invention, "health functional food" refers to a food manufactured and processed using raw materials or ingredients useful for the human body. Here, the term 'functionality' refers to obtaining useful effects for health purposes such as regulating nutrients or physiological effects on the structure and function of the human body.
본 발명은 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 함유하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition comprising, as an active ingredient, a mixed fermented extract of grains and seaweeds fermented with Enterococcus faecium MSS2.
바람직하게는, 상기 엔테로코쿠스 파에시움 MSS2는 기탁번호 KCTC 12795BP로 기탁된 기탁 균주일 수 있고, 상온 발효 균주이다.Preferably, the Enterococcus faecium MSS2 may be a deposited strain deposited with accession number KCTC 12795BP, and is a room temperature fermentation strain.
바람직하게는, 상기 곡류는 팥, 조, 수수 및 기장으로 이루어진 군에서 선택된 하나 이상의 곡류일 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain may be one or more grains selected from the group consisting of red bean, millet, sorghum and millet, but is not limited thereto.
바람직하게는, 상기 해조류는 톳, 김, 다시마, 미역, 꼬시래기, 도박 및 큰실말로 이루어진 군에서 선택된 하나 이상의 해조류일 수 있으나, 이에 한정되는 것은 아니다.Preferably, the seaweed may be one or more seaweeds selected from the group consisting of seaweed, seaweed, kelp, seaweed, seaweed, gambler, and seaweed, but is not limited thereto.
바람직하게는, 상기 곡류 및 해조류 혼합 발효 추출물은 항산화, 보습, 미백, 주름개선 및 항노화로 이루어진 군에서 선택된 하나 이상의 기능성을 가질 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain and seaweed mixed fermented extract may have one or more functions selected from the group consisting of antioxidant, moisturizing, whitening, wrinkle improvement and anti-aging, but is not limited thereto.
바람직하게는, 상기 곡류 및 해조류 혼합 발효 추출물은 곡류 및 해조류 혼합물의 물 추출물을 엔테로코쿠스파에시움 MSS2로 상온 발효하여 얻을 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain and seaweed mixed fermented extract can be obtained by fermenting a water extract of the grain and seaweed mixture with Enterococcus sphaecium MSS2 at room temperature, but is not limited thereto.
본 발명에 따른 화장료 조성물은 식품 의약품 안전청에 등록된 화장품 원료 리스트 및 ICID(국제화장품 원료집)에 등재되어 있는 성분 중에서 1종 이상을 더 포함할 수 있다. 예를 들어, 상기 화장료 조성물은 유기 용매, 용해제, 농축제, 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 방향제, 계면활성제, 유화제, 충전제, 금속이온 봉쇄제, 방부제, 비타민, 차단제, 습윤화제, 염료 및 안료, 친수성 또는 친유성 활성제, 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품 분야에서 통상적으로 사용되는 보조제를 하나 이상 더 포함할 수 있다.The cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Food and Drug Administration and ICID (International Cosmetic Ingredients). For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.
상기 비타민은 수용성 또는 유용성 비타민을 포함할 수 있고, 상기 수용성 비타민은 수용성 비타민으로서 화장품에 배합 가능한 것으로, 바람직하게는 비타민 B1, 비타민 B2, 비타민 B6, 피리독신, 염산피리독신, 비타민 B12, 판토텐산, 니코틴산, 니코틴산아미드, 엽산, 비타민C, 비타민 H 등을 들 수 있으며, 보다 바람직하게는 콜라겐 합성이 도움을 주는 비타민 C를 포함할 수 있다. 또한, 그들의 염 (티아민염산염, 아스코르빈산나트륨염 등)이나 유도체(아스코르빈산-2-인산나트륨염, 아스코르빈산-2-인산마그네슘염 등)도 포함될 수 있고, 상기 수용성 비타민은 미생물 변환법, 미생물의 배양물로부터의 정제법, 효소법 또는 화학 합성법 등의 통상의 방법에 의해 수득될 수 있다.The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis. In addition, salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) may be included, and the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as purification from a culture of microorganisms, enzymatic method or chemical synthesis method.
본 발명에 따른 화장료 조성물은 본 발명이 속하는 기술 분야에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있다. 예를 들어, 스킨로션, 스킨소프너, 스킨토너, 겔, 크림, 로션, 밀크로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 팩, 마사지 크림 및 스프레이 등으로 제형화 될 수 있으나, 이에 한정되는 것은 아니다. 더욱 상세하게는, 영양 크림, 마사지 크림, 에센스, 아이 크림, 선로션, 선크림, 메이크업 프라이머, 메이크업 베이스, 비비크림, 클렌징크림, 클렌징폼, 클렌징 워터, 팩, 스틱상 제품, 밤(Balm) 타입 제품, 스프레이 또는 파우더의 제형으로 제조될 수 있다.The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, skin lotions, skin softeners, skin toners, gels, creams, lotions, milk lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays. and the like, but is not limited thereto. More specifically, nourishing cream, massage cream, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick product, balm type It may be prepared in the form of a product, spray or powder.
본 발명에 따른 화장료 조성물은 단독 또는 중복으로 도포하여 사용하거나, 본 발명 이외의 다른 화장료 조성물과 중복 도포하여 사용할 수 있다. 또한, 본 발명에 따른 화장료 조성물은 통상적인 사용방법에 따라 사용될 수 있으며, 사용자의 피부 상태 또는 취향에 따라 그 사용횟수를 달리할 수 있다.The cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.
또한, 본 발명은 엔테로코쿠스 파에시움 MSS2로 발효한 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 함유하는 건강기능식품 조성물을 제공한다.In addition, the present invention provides a health functional food composition containing, as an active ingredient, a mixed fermented extract of grains and seaweeds fermented with Enterococcus faecium MSS2.
본 발명에 따른 건강기능식품은 분말, 과립, 정제, 캡슐, 시럽 또는 음료 등으로 제조될 수 있고, 본 발명에 따른 곡류 및 해조류 혼합 발효 추출물 이외에 다른 식품 또는 식품 첨가물과 함께 제조될 수 있다. 즉, 상기 건강기능식품은 당 업계에서 통상적으로 사용되는 방법에 의하여 제조가능하고, 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 또한, 상기 건강기능식품 제조 시에는 당 업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있으며 통상적인 방법에 따라 적절하게 사용될 수 있다.The health functional food according to the present invention may be manufactured in powder, granule, tablet, capsule, syrup or beverage, and the like, and may be prepared together with other food or food additives in addition to the mixed fermented extract of grain and seaweed according to the present invention. That is, the health functional food can be manufactured by a method commonly used in the art, and can be manufactured without limitation as long as it is a formulation recognized as a health functional food. In addition, when manufacturing the health functional food, it can be prepared by adding raw materials and components commonly added in the art, and can be appropriately used according to a conventional method.
본 발명에 따른 건강기능식품은 일반 약품과는 달리 곡류 및 해조류를 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 피부 주름 개선, 피부 탄력 증진 또는 피부 미백 효과를 증진시키기 위한 보조제로 섭취될 수 있다.The health functional food according to the present invention has the advantage that there are no side effects that may occur when taking the drug for a long period of time by using grains and seaweed as raw materials, unlike general drugs, and has excellent portability to improve skin wrinkles, improve skin elasticity, or improve skin elasticity. It can be taken as an adjuvant to enhance the whitening effect.
본 발명에 따른 건강기능식품 조성물에서, 유효성분인 상기 곡류 및 해조류 혼합 발효 추출물의 혼합양은 그의 사용 목적, 예를 들어, 예방, 건강 또는 치료적 처치에 따라 적합하게 결정될 수 있다. In the health functional food composition according to the present invention, the mixing amount of the grain and seaweed mixed fermented extract as an active ingredient may be suitably determined according to the purpose of its use, for example, preventive, health or therapeutic treatment.
본 발명에 따른 건강기능식품이 취할 수 있는 형태에는 제한이 없으며, 통상적인 의미의 식품을 모두 포함할 수 있고, 기능성 식품 등 당업계에 알려진 용어와 혼용 가능하다. 아울러 본 발명에 따른 곡류 및 해조류 혼합 발효 추출물을 유효성분으로 포함하는 건강기능식품은 당업자의 선택에 따라 식품에 함유될 수 있는 적절한 기타 보조성분과 공지의 첨가제를 혼합하여 제조할 수 있다. 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 쵸코렛, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 본 발명에 따른 곡류 및 해조류 혼합 발효 추출물을 주성분으로 하여 제조한 즙, 차, 젤리 및 주스 등에 첨가하여 제조할 수 있다. 또한, 동물을 위한 사료로 이용되는 식품도 포함할 수 있다.There is no limitation in the form that the health functional food according to the present invention can take, and it may include any food in a conventional sense, and may be used interchangeably with terms known in the art, such as functional food. In addition, the health functional food containing the mixed fermented extract of grains and seaweed according to the present invention as an active ingredient can be prepared by mixing other suitable auxiliary ingredients that may be contained in food and known additives according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There is a vitamin complex, and it can be prepared by adding the mixed fermented extract of grain and seaweed according to the present invention as a main component to juice, tea, jelly, juice, and the like. It may also include food used as feed for animals.
또한, 본 발명은 (1) 자연 건조한 곡류 및 해조류 혼합 분말에 물을 첨가하고 100~130℃에서 추출하는 단계; (2) 상기 추출된 추출물에 엔테로코쿠스 파에시움 MSS2을 접종하고 상온 발효하는 단계; 및 (3) 상기 발효된 발효물을 원심분리 하여 상층액을 회수하고 필터로 여과하여 균체와 곡류 및 해조류 분말을 제거한 발효물을 제조하는 단계를 포함하는, 곡류 및 해조류 혼합 발효 추출물의 제조방법을 제공한다.In addition, the present invention comprises the steps of (1) adding water to a naturally dried grain and seaweed mixed powder and extracting it at 100 ~ 130 ℃; (2) inoculating the extracted extract with Enterococcus faecium MSS2 and fermenting at room temperature; and (3) centrifuging the fermented fermented product to recover the supernatant and filtering it through a filter to prepare a fermented product from which cells and grains and seaweed powder are removed. to provide.
바람직하게는, 상기 (1) 단계의 곡류 및 해조류 혼합 분말은 곡류 분말 및 해조류 분말을 1 ~ 5 : 1의 중량비로 혼합할 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain and seaweed mixed powder of step (1) may be mixed with the grain powder and seaweed powder in a weight ratio of 1 to 5:1, but is not limited thereto.
바람직하게는, 상기 엔테로코쿠스 파에시움 MSS2는 기탁번호 KCTC 12795BP로 기탁된 기탁 균주일 수 있고, 상온 발효 균주이다.Preferably, the Enterococcus faecium MSS2 may be a deposited strain deposited with accession number KCTC 12795BP, and is a room temperature fermentation strain.
바람직하게는, 상기 곡류는 팥, 조, 수수 및 기장으로 이루어진 군에서 선택된 하나 이상의 곡류일 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain may be one or more grains selected from the group consisting of red bean, millet, sorghum and millet, but is not limited thereto.
바람직하게는, 상기 해조류는 톳, 김, 다시마, 미역, 꼬시래기, 도박 및 큰실말로 이루어진 군에서 선택된 하나 이상의 해조류일 수 있으나, 이에 한정되는 것은 아니다.Preferably, the seaweed may be one or more seaweeds selected from the group consisting of seaweed, seaweed, kelp, seaweed, seaweed, gambler, and seaweed, but is not limited thereto.
바람직하게는, 상기 곡류 및 해조류 혼합 발효 추출물은 항산화, 보습, 미백, 주름개선 및 항노화로 이루어진 군에서 선택된 하나 이상의 기능성을 가질 수 있으나, 이에 한정되는 것은 아니다.Preferably, the grain and seaweed mixed fermented extract may have one or more functions selected from the group consisting of antioxidant, moisturizing, whitening, wrinkle improvement and anti-aging, but is not limited thereto.
이하, 본 발명의 이해를 돕기 위하여 실시예를 들어 상세하게 설명하기로 한다. 다만 하기의 실시예는 본 발명의 내용을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다. 본 발명의 실시예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다.Hereinafter, to help the understanding of the present invention, examples will be described in detail. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.
<< 실시예Example 1> 팥(곡류) 및 톳(해조류)을 이용한 발효 추출물 제조 1> Manufacture of fermented extract using red bean (grain) and shiitake (seaweed)
곡류 중에서 적당하게 건조하여 분말화 한 팥과, 해조류 중에서 적당하게 잘 건조하여 분말화 한 톳을 각각 3 : 1 비율로 하는 복합 물질과 증류수(Distilled water)를 혼합하여 사용하였다. 상기 혼합물 중에서 유효성분을 추출하기 위해 121℃에서 15분 정도 추출을 진행하였다. 상기 추출된 추출물에 엔테로코쿠스 파에시옴(Enterococcus Faecium) MSS2 균주(기탁번호: KCTC 12795BP) 1 × 106 CFU/ml를 접종하고 발효가 잘 일어나도록 진탕 배양기를 이용하여 30℃에서 50 rpm 조건으로 72시간 동안 발효시켰다. 발효 과정을 거쳐서 수확한 액상 혼합 발효물은 원심 분리기를 이용하여 1000 rpm에서 10 ~ 20 분간 원심 분리시켜 상층액을 회수하였다. 상기 회수한 상층액은 필터 페이퍼(0.072 mm)를 이용하여 균체 및 팥 및 톳 분말을 제거하고, 얻어진 발효추출물은 -50℃, 5 ~ 10 mmHg 조건에서 동결 건조하여 분말 형태로 제조하였다(도 1).A mixture of red beans, which were dried and powdered in grains, and red beans, which were dried and powdered in seaweed, in a ratio of 3:1, respectively, and distilled water were mixed and used. In order to extract the active ingredient from the mixture, extraction was performed at 121° C. for about 15 minutes. Enterococcus Faecium MSS2 strain (Accession No.: KCTC 12795BP) 1 × 10 6 CFU / ml inoculated into the extracted extract and using a shaking incubator for fermentation to occur well at 30 ℃ 50 rpm conditions was fermented for 72 hours. The liquid mixture fermented product harvested through the fermentation process was centrifuged at 1000 rpm for 10 to 20 minutes using a centrifuge to recover the supernatant. The recovered supernatant was prepared in the form of a powder by using filter paper (0.072 mm) to remove cells, red bean, and hibiscus powder, and freeze-drying the obtained ferment extract at -50°C and 5 to 10 mmHg conditions (FIG. 1). ).
<< 실시예Example 2> 혼합 발효 추출물의 발효 시간별 발효 추출물의 pH 변화 2> pH change of fermented extract by fermentation time of mixed fermented extract
발효추출물을 filter paper(0.072mm)를 이용하여 균체 및 팥 및 톳 분말을 제거하고 pH meter기를 사용하여 pH를 측정하였다(도 2).Fermented extracts were removed by using filter paper (0.072mm) to remove cells and red bean and hibiscus powder, and the pH was measured using a pH meter (FIG. 2).
<< 실시예Example 3> 혼합 발효 추출물의 총 페놀 함량 분석 3> Analysis of total phenol content of mixed fermented extracts
발효추출물 10 μl와 0.2N Folin-Ciocalteu reagent 50 ㎕를 96 well plate에서 함께 혼합한다. 5 분 동안 반응 시킨 후, 7.5 % 탄산나트륨 용액 40㎕를 첨가하였다. 혼합 용액을 실온에서 30 분 반응시킨다. 흡광도를 마이크로 웨이브 다중 웰 플레이트 판독기 (Tecan F-200, Mannedorf, Zurich, Swit-Zerland)를 사용하여 730 nm에서 측정 하였다. 표준곡선은 0 - 90 μg / ml 범위의 Galic acid 표준 용액을 사용하여 설정하였다(도 3).10 μl of the fermentation extract and 50 μl of 0.2N Folin-Ciocalteu reagent are mixed together in a 96 well plate. After reacting for 5 minutes, 40 μl of a 7.5% sodium carbonate solution was added. The mixed solution is reacted at room temperature for 30 minutes. Absorbance was measured at 730 nm using a microwave multi-well plate reader (Tecan F-200, Mannedorf, Zurich, Swit-Zerland). The standard curve was set using a standard solution of galic acid in the range of 0 - 90 μg / ml (Fig. 3).
<< 실시예Example 4> 혼합 발효 추출물의 총 플라보노이드 함량 분석 4> Analysis of total flavonoid content of mixed fermented extracts
50㎕의 발효추출물과 50㎕의 2 % AlCl3 시약을 96 well plate에서 혼합하였다. 혼합 된 용액을 암실에서 30 분 동안 반응시켰다. 반응시킨 후 흡광도는 마이크로 웨이브 다중 웰 플레이트 판독기를 사용하여 450 nm에서 측정하였다. 표준곡선은 0 - 90 μg / ml 범위의 Quercetin 표준 용액을 사용하여 설정하였다(도 4).50 μl of fermentation extract and 50 μl of 2% AlCl 3 reagent were mixed in a 96 well plate. The mixed solution was reacted for 30 min in the dark. After the reaction, the absorbance was measured at 450 nm using a microwave multi-well plate reader. A standard curve was established using a standard solution of Quercetin in the range of 0 - 90 μg / ml (Fig. 4).
<< 실시예Example 5> 혼합 발효 추출물의 5> of mixed fermented extracts DPPHDPPH 라디칼 소거 효과 분석 Analysis of radical scavenging effect
DPPH 9mg을 메탄올 30 ml에 녹인 후 실온에서 발효 추출물 40㎕, DPPH 용액 40㎕, MeOH 120㎕를 혼합하고 30 분간 반응시킨다. 그리고 다중 웰 플레이트 판독기 (Tecan F-200, Mannedorf, Zurich, Swit-Zerland)를 사용하여 517nm에서 측정 하였다(도 5).After dissolving 9 mg of DPPH in 30 ml of methanol, 40 μl of ferment extract, 40 μl of DPPH solution, and 120 μl of MeOH were mixed at room temperature and reacted for 30 minutes. Then, measurements were made at 517 nm using a multi-well plate reader (Tecan F-200, Mannedorf, Zurich, Swit-Zerland) (Fig. 5).
<< 실시예Example 6> 혼합 발효 추출물의 6> of mixed fermented extracts 티로시네이즈tyrosinase 억제 효과 분석 Inhibitory effect analysis
발효추출물 10 ㎕, tyrosinase (110 units / ml) 20 μL와 1.5 mM L-DOPA 용액, 50 mM sodium phosphate buffer (pH 6.5) 및 증류수를 10 : 10 : 1의 비율로 함유된 혼합물 170㎕에 넣었다. 다음 혼합물을 37℃에서 10 분간 반응시킨 후, 혼합물의 흡광도를 Tecan F-200 멀티 웰 플레이트 판독기를 사용하여 450 nm에서 측정하였다(도 6).10 μl of fermentation extract, 20 μL of tyrosinase (110 units / ml), 1.5 mM L-DOPA solution, 50 mM sodium phosphate buffer (pH 6.5) and distilled water were added to 170 μl of a mixture containing 10: 10: 1 ratio. After reacting the mixture at 37°C for 10 minutes, the absorbance of the mixture was measured at 450 nm using a Tecan F-200 multi-well plate reader (FIG. 6).
<< 실시예Example 7> 혼합 발효 추출물의 7> Mixed fermented extracts 콜라게네이즈Collagenase 억제 효과 분석 Inhibitory effect analysis
콜라게나제 저해 활성은 collagenase activity colorimetric assay kit (K792, Biovision, USA)를 사용하여 평가 하였다. 다중 웰 플레이트 판독기 (Tecan F-200, Mannedorf, Zurich, Swit-Zerland)를 사용하여 345nm에서 측정하였다(도 7).Collagenase inhibitory activity was evaluated using a collagenase activity colorimetric assay kit (K792, Biovision, USA). Measurements were made at 345 nm using a multi-well plate reader (Tecan F-200, Mannedorf, Zurich, Swit-Zerland) (FIG. 7).
<< 실시예Example 8> 혼합 발효 추출물의 8> Mixed fermented extracts 엘라스테이즈Elastase 억제 효과 분석 Inhibitory effect analysis
96 well plate에 0.1M Tris-HCl buffer 80㎕, 발효추출물 40㎕, 3mM N-succinyl-(Ala)3-P-nitroanilide 30㎕, 0.2 unit Elastase 20㎕를 혼합한 후, 30℃에서 20 분 동안 인큐베이션하고, 흡광도를 Tecan F-200 멀티 웰 플레이트 판독기를 사용하여 410 nm에서 측정 하였다(도 8).After mixing 80 μl of 0.1M Tris-HCl buffer, 40 μl of fermentation extract , 30 μl of 3mM N-succinyl-(Ala) 3- P-nitroanilide, and 20 μl of 0.2 unit Elastase in a 96 well plate, at 30℃ for 20 minutes After incubation, absorbance was measured at 410 nm using a Tecan F-200 multi-well plate reader (Fig. 8).
<< 실시예Example 9> 혼합 발효 추출물의 9> of mixed fermented extracts 멜라노마melanoma 세포 및 섬유아세포 cells and fibroblasts 생존률survival rate 분석 analysis
B16F10 melanoma 세포와 CCD 986SK 세포를 96 well plate에서 1 × 105 cell/ml의 농도로 배양하였다. 24 시간 후, 다양한 농도의 샘플로 처리하였다. 24 시간 동안 배양한 후, 세포를 PBS로 1 회 세척하고 100㎕의 MTT 용액 (5mg/ml)을 각 well에 첨가하여 3시간 동안 반응시킨다. 이후 MTT 용액을 제거하고 DMSO (dimethyl sulfoxide) 100㎕를 가하였다. 흡광도를 마이크로웨이브 다중 웰 플레이트 판독기 (Tecan F-200, Mannedorf, Zurich, Swit-Zerland)를 사용하여 540 nm에서 측정하였다(도 9 및 도 10).B16F10 melanoma cells and CCD 986SK cells were cultured in a 96 well plate at a concentration of 1 × 10 5 cell/ml. After 24 hours, samples of various concentrations were treated. After culturing for 24 hours, the cells are washed once with PBS, and 100 μl of MTT solution (5 mg/ml) is added to each well and reacted for 3 hours. Then, the MTT solution was removed and 100 μl of DMSO (dimethyl sulfoxide) was added thereto. Absorbance was measured at 540 nm using a microwave multi-well plate reader (Tecan F-200, Mannedorf, Zurich, Swit-Zerland) ( FIGS. 9 and 10 ).
<< 실시예Example 10> 혼합 발효 추출물의 멜라닌 함량 분석 10> Analysis of melanin content of mixed fermented extracts
B16F10 흑색종 세포 (1 × 105 cell/ml)를 24 well plate에 배양 후 100 μM α-MSH의 존재 또는 부재하에 배양 하였다. 이후 세포를 다양한 농도의 발효추출물(10, 25, 50 ㎍/ml)과 함께 72 시간 동안 배양했다. 발효추출물을 PBS로 두 번 세척 한 다음 100μL의 1N NaOH에 용해시켰다. 용해시킨 세포를 80℃에서 1 시간 동안 반응시킨 후 흡광도를 Tecan F-200 멀티 웰 플레이트 판독기를 사용하여 450 nm에서 측정 하였다(도 11).B16F10 melanoma cells (1 × 10 5 cell/ml) were cultured in a 24-well plate and then cultured in the presence or absence of 100 μM α-MSH. The cells were then incubated with various concentrations of the ferment extract (10, 25, 50 μg/ml) for 72 hours. The fermentation extract was washed twice with PBS and then dissolved in 100 μL of 1N NaOH. After the lysed cells were reacted at 80° C. for 1 hour, absorbance was measured at 450 nm using a Tecan F-200 multi-well plate reader ( FIG. 11 ).
<< 실시예Example 11> 혼합 발효 추출물의 세포 내 11> Intracellular of mixed fermented extracts 티로시네이즈tyrosinase 활성 분석 activity assay
세포를 24 well plate에 1 × 104 cell/ml의 농도로 배양하였다. B16F10 흑색종 세포를 100 μM α-MSH의 존재 또는 부재하에 배양 한 다음, 이들을 다양한 농도의 발효 추출물 (10, 50, 100 ㎍/ml)로 72 시간 처리하였다. 이후 상층액을 제거하고 1 % Triton X-100 (Sigma-Aldrich, MO, USA) 및 0.1 mM PMSF (phenylmethylsulfonyl fluoride)를 함유한 50 mM sodium phosphate buffer (pH 6.5) 100 μl 분주하여 cell을 수거한다. 이후 -80℃에서 30분간 냉동 후 해동을 3번 반복한다, 세포 추출물을 4℃에서 30 분 동안 12,000 rpm에서 원심 분리시켰다. 단백질 농도는 BCA Protein Assay kit를 기준으로 Bradford 법으로 측정하였다. 96 웰 플레이트의 각 웰에 0.1 μM PBS (pH 6.8)에 녹인 50μg/100ul의 단백질과 1.5 mM L-DOPA 100 μl로 구성된 반응 혼합물을 37 ℃에서 1시간 반응시킨 후, 흡광도를 Tecan F-200 멀티 웰 플레이트 판독기를 사용하여 475 nm에서 측정하였다(도 12).Cells were cultured in a 24 well plate at a concentration of 1 × 10 4 cell/ml. B16F10 melanoma cells were cultured in the presence or absence of 100 μM α-MSH, and then they were treated with various concentrations of ferment extract (10, 50, 100 μg/ml) for 72 hours. Then, the supernatant is removed, and 100 μl of 50 mM sodium phosphate buffer (pH 6.5) containing 1% Triton X-100 (Sigma-Aldrich, MO, USA) and 0.1 mM PMSF (phenylmethylsulfonyl fluoride) is dispensed to collect cells. After freezing at -80°C for 30 minutes, thawing was repeated 3 times, and the cell extract was centrifuged at 12,000 rpm at 4°C for 30 minutes. Protein concentration was measured by the Bradford method based on the BCA Protein Assay kit. In each well of a 96-well plate, a reaction mixture consisting of 50 μg/100 μl of protein and 100 μl of 1.5 mM L-DOPA dissolved in 0.1 μM PBS (pH 6.8) was reacted at 37 ° C for 1 hour, and the absorbance was measured with Tecan F-200 Multi Measurements were made at 475 nm using a well plate reader ( FIG. 12 ).
<< 제제예Formulation example 1> 로션의 제조 1> Preparation of lotion
글리세린 5.0 중량부, 다이프로필렌글라이콜 3.0 중량부, 소듐아크릴레이크 코폴리머/레시틴 0.3 방부제 미량과 잔량의 정제수를 혼합교반하여 75 내지 80℃로 가열하여 제조부에 투입한 후 유화기를 작용시키고 세테아릴올리베이트 1.0 중량부, 하이드로제네이티드폴리데센 2.0 중량부, 폴리글리세릴-3메칠글루코오스디스테아레이트 1.5 중량부, 소르비탄올리베이트 1.0 중량부 75 내지 80℃로 가열하여 투입한 뒤 유화 하였다. 유화가 끝나면 교반기를 이용하여 교반하면서 45℃까지 열 냉각한 뒤 향료를 미량을 투입하고, 35℃에서 실시예 1에 따른 팥 및 톳 혼합 발효 추출물을 투입하여 25℃까지 냉각한 뒤 숙성시켰다. 5.0 parts by weight of glycerin, 3.0 parts by weight of dipropylene glycol, 0.3 parts by weight of sodium acrylic lake copolymer/lecithin, a small amount of preservative and the remaining amount of purified water are mixed and stirred, heated to 75 to 80° C. Tearyl olivate 1.0 parts by weight, hydrogenated polydecene 2.0 parts by weight, polyglyceryl-3-methylglucose distearate 1.5 parts by weight, and sorbitan olivate 1.0 parts by weight were heated to 75 to 80° C. and then emulsified. . After the emulsification was finished, the mixture was cooled to 45°C while stirring using a stirrer, and then a small amount of spice was added, and the red bean and shiitake mixed fermented extract according to Example 1 was added at 35°C, cooled to 25°C, and then aged.
<< 제제예Formulation example 2> 크림의 제조 2> Preparation of cream
글리세린 3.0 중량부, 다이프로필렌글라이콜 2.0 중량부 소듐아크릴레이크 코폴리머/레시틴 0.5 방부제 미량과 잔량의 정제수를 혼합교반하여 75 내지 80℃로 가열하여 제조부에 투입한 후 유화기를 작용시키고 하이드로제네이티드폴리데센 2.0 중량부, 폴리글리세릴-3메칠글루코오스디스테아레이트 2.5 중량부, 소르비탄스테아레이트 2.4 중량부, 소르비탄올리베이트 1.8 중량부 75 내지 80℃로 가열하여 투입한 뒤 유화하였다. 유화가 끝나면 교반기를 이용하여 교반하면서 45℃까지 열 냉각한 뒤 향료를 미량을 투입하고, 35℃에서 실시예 1에 따른 팥 및 톳 혼합 발효 추출물을 투입하여 25℃까지 냉각한 뒤 숙성시켰다.3.0 parts by weight of glycerin, 2.0 parts by weight of dipropylene glycol Sodium acrylate copolymer / lecithin 0.5 A trace amount of preservative and the remaining amount of purified water are mixed and stirred, heated to 75 to 80 ° C. 2.0 parts by weight of tidpolydecene, 2.5 parts by weight of polyglyceryl-3-methylglucose distearate, 2.4 parts by weight of sorbitan stearate, and 1.8 parts by weight of sorbitan olivate were added by heating at 75 to 80° C. and then emulsified. After the emulsification was finished, the mixture was cooled to 45°C while stirring using a stirrer, and then a small amount of spice was added, and the red bean and shiitake mixed fermented extract according to Example 1 was added at 35°C, cooled to 25°C, and then aged.
<< 제제예Formulation example 3> 토너의 제조 3> Preparation of toner
글리세린 3.0 중량부, 다이프로필렌글라이콜 5.0 중량부, 프로판디올 1.0 중량부, 하이드록시에칠셀룰로오스 0.05 중량부 방부제 미량과 잔량의 정제수를 혼합교반하여 75 내지 80℃로 가열하여 제조부에 투입한 후 가용화기를 작용시키고 피이지-60하이드로제네이티드캐스터오일 0.2 중량부, 에탄올 2.0 중량부, 올리브오일피이지-7에스터 0.01 중량부, 향료 미량 함께 75 내지 80℃로 가열하여 투입한 뒤 가용화 하였다. 가용화가 끝나면 교반기를 이용하여 교반하면서 35℃까지 열 냉각한 뒤 실시예 1에 따른 팥 및 톳 혼합 발효 추출물을 투입하여 25℃까지 냉각한 뒤 숙성시켰다.3.0 parts by weight of glycerin, 5.0 parts by weight of dipropylene glycol, 1.0 parts by weight of propanediol, 0.05 parts by weight of hydroxyethyl cellulose A trace amount of preservative and the remaining amount of purified water were mixed and stirred, heated to 75 to 80 ° C. After the solubilizer was activated, 0.2 parts by weight of PEG-60 hydrogenated castor oil, 2.0 parts by weight of ethanol, 0.01 parts by weight of olive oil PEG-7 ester, and a trace amount of fragrance were added together by heating at 75 to 80° C., followed by solubilization. After solubilization, the mixture was cooled to 35° C. while stirring using a stirrer, and then the red bean and turmeric mixed ferment extract according to Example 1 was added, cooled to 25° C., and then aged.
<< 제제예Formulation example 4> 4> 세럼의of serum 제조 manufacturing
글리세린 7.0 중량부, 프로판디올 4.0 중량부, 하이드록시에칠아크릴레이트/소듐아크릴로일디메칠타우레이트코폴리머 0.2 중량부, 데하이드로잔탄검 0.02 중량부, 하이드록시에칠셀룰로오스 0.5 중량부 방부제 미량과 잔량의 정제수를 혼합교반하여 75 내지 80℃로 가열하여 제조부에 투입한 후 가용화기를 작용시키고 피이지-60하이드로제네이티드캐스터오일 0.2 중량부, 향료 미량 함께 75 내지 80℃로 가열하여 투입한 뒤 가용화 하였다. 가용화가 끝나면 교반기를 이용하여 교반하면서 35℃까지 열 냉각한 뒤 실시예 1에 따른 팥 및 톳 혼합 발효 추출물을 투입하여 25℃까지 냉각한 뒤 숙성시켰다.7.0 parts by weight of glycerin, 4.0 parts by weight of propanediol, 0.2 parts by weight of hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer, 0.02 parts by weight of dehydroxanthan gum, 0.5 parts by weight of hydroxyethylcellulose After mixing and stirring the remaining amount of purified water, it is heated to 75 to 80° C., put into the manufacturing unit, and then the solubilizer is activated, and 0.2 parts by weight of PEG-60 Hydrogenated Castor Oil and a trace amount of fragrance are heated to 75 to 80° C. Solubilized. After solubilization, the mixture was cooled to 35° C. while stirring using a stirrer, and then the red bean and turmeric mixed ferment extract according to Example 1 was added, cooled to 25° C., and then aged.
<< 제제예Formulation example 5> 5> 워시폼의of wash foam 제조 manufacturing
TEA-코코일 글루타메이트 30.0 중량부, 디소듐 라우레스 설포숙시네이트글리세린 10.0 중량부, 글리세린 10.0 중량부, 코카마이드 DEA 2.0 중량부, PEG-120 메칠글루코오스 디올리에이트 1.0 중량부, 메칠글루세스-20 0.5 중량부, PEG-150 펜타에리트리틸 테트라 스테아레이트 0.5 중량부, 테트라소듐 EDTA 0.05 중량부 및 방부제 미량을 순차적으로 제조부에 투입하고 60 내지 65℃로 가열한 후 15분 동안 교반하였다. 교반이 끝나면 정제수의 일부를 투입하여 30분 동안 교분한 후, 다시 정제수의 일부를 천천히 투입하고 30분 동안 교반한 후 35℃까지 냉각하고, 실시예 1에 따른 팥 및 톳 혼합 발효 추출물과 향료를 투입하여 25℃까지 냉각한 뒤 숙성시켰다.TEA-cocoyl glutamate 30.0 parts by weight, disodium laureth sulfosuccinate glycerin 10.0 parts by weight, glycerin 10.0 parts by weight, cocamide DEA 2.0 parts by weight, PEG-120 methylglucose dioleate 1.0 parts by weight, methylgluces- 20 0.5 parts by weight, PEG-150 pentaerythrityl tetrastearate 0.5 parts by weight, tetrasodium EDTA 0.05 parts by weight, and a trace amount of preservative were sequentially added to the preparation unit, heated to 60 to 65° C., and stirred for 15 minutes. After stirring, a part of purified water was added and the mixture was stirred for 30 minutes, then a part of purified water was slowly added again, stirred for 30 minutes, cooled to 35° C. After cooling to 25°C, it was aged.
<< 제제예Formulation example 6> 건강식품의 제조 6> Manufacture of health food
실시예 1에 따른 팥 및 톳 혼합 발효 추출물 1 ㎎, 비타민 혼합물 적량(비타민 A 아세테이트 70 ㎍, 비타민 E 1.0 ㎎, 비타민 B 1 0.13 ㎎, 비타민 B 2 0.15 ㎎, 비타민 B 6 0.5 ㎎, 비타민 B 12 0.2 ㎍, 비타민 C 10 ㎎, 비오틴 10 ㎍, 니코틴산아미드 1.7㎎, 엽산 50 ㎍, 판토텐산 칼슘 0.5 ㎎) 및 무기질 혼합물 적량(황산제1철 1.75 ㎎, 산화아연 0.82 ㎎, 탄산마그네슘 25.3 ㎎, 제1인산칼륨 15 ㎎, 제2인산칼슘 55 ㎎, 구연산칼륨 90 ㎎, 탄산칼슘 100 ㎎, 염화마그네슘 24.8 ㎎)을 혼합한 다음 과립을 제조하고 통상의 방법에 따라 건강식품을 제조하였다.1 mg of mixed fermented extract of red bean and hibiscus according to Example 1, appropriate amount of vitamin mixture (vitamin A acetate 70 μg, vitamin E 1.0 mg,
<< 제제예Formulation example 7> 건강음료의 제조 7> Manufacture of health drinks
실시예 1에 따른 팥 및 톳 혼합 발효 추출물 1 ㎎, 구연산 1000 ㎎, 올리고당 100 g, 매실농축액 2 g, 타우린 1 g 및 정제수를 가하여 전체 900 ㎖가 되도록 하며, 통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1시간 동안 85℃에서 교반가열한 후, 만들어진 용액을 여과하여 멸균된 2 L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관하였다.1 mg of the mixed fermented extract of red bean and hibiscus according to Example 1, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, 1 g of taurine and purified water were added to make a total of 900 ml. After mixing the components of the mixture and heating at 85 ℃ for about 1 hour, the resulting solution was filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then stored in a refrigerator.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
Claims (13)
(2) 상기 추출된 추출물에 기탁번호 KCTC 12795BP로 기탁된 엔테로코쿠스 파에시움 MSS2을 접종하고 상온 발효하는 단계; 및
(3) 상기 발효된 발효물을 원심분리 하여 상층액을 회수하고 필터로 여과하여 균체와 곡류 및 해조류 분말을 제거한 발효물을 제조하는 단계를 포함하는, 팥 및 톳 혼합 발효 추출물의 제조방법.(1) adding water to the naturally dried red bean and shiitake mixed powder and extracting at 100~130℃;
(2) inoculating the extracted extract with Enterococcus faecium MSS2 deposited with accession number KCTC 12795BP and fermenting at room temperature; and
(3) centrifuging the fermented fermented product to recover the supernatant and filtering with a filter to prepare a fermented product from which cells and grains and seaweed powder are removed.
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