KR102162684B1 - Manufacturing method of seasoning comprising powdered larva of Protaetia brevitarsis and seasoning manufactured thereby - Google Patents
Manufacturing method of seasoning comprising powdered larva of Protaetia brevitarsis and seasoning manufactured thereby Download PDFInfo
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- KR102162684B1 KR102162684B1 KR1020180121178A KR20180121178A KR102162684B1 KR 102162684 B1 KR102162684 B1 KR 102162684B1 KR 1020180121178 A KR1020180121178 A KR 1020180121178A KR 20180121178 A KR20180121178 A KR 20180121178A KR 102162684 B1 KR102162684 B1 KR 102162684B1
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- extract
- seasoning
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- white spotted
- radish larva
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
- A23L27/11—Natural spices, flavouring agents or condiments; Extracts thereof obtained by solvent extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
- A23L27/14—Dried spices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/204—Animal extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법 및 그에 의해 제조된 조미료에 관한 것으로, 더욱 상세하게는 흰점박이꽃무지 유충 분말 및 식물 추출물을 유효성분으로 함유한 항당뇨 활성 식품 조미료 및 그에 의해 제조된 조미료에 관한 것이다.
본 발명에 따르면, 당뇨 유발 동물에서 혈당을 현저히 강하시킬 수 있어 당뇨개선용의 건강식품 및 조미료에 유용하게 활용할 수 있다.The present invention relates to a method for producing a seasoning containing white spotted radish larva powder and to a seasoning prepared thereby, and more particularly, antidiabetic active food seasoning containing white spotted radish larva powder and plant extracts as active ingredients And to a seasoning prepared thereby.
According to the present invention, it is possible to significantly lower blood sugar in diabetic animals, and thus can be usefully used in health foods and seasonings for improving diabetes.
Description
본 발명은 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법 및 그에 의해 제조된 조미료에 관한 것으로, 더욱 상세하게는 흰점박이꽃무지 유충 분말 및 식물 추출물을 유효성분으로 함유한 항당뇨 활성 식품 조미료 및 그에 의해 제조된 조미료에 관한 것이다.The present invention relates to a method for producing a seasoning containing white spotted radish larva powder and to a seasoning prepared thereby, and more particularly, antidiabetic active food seasoning containing white spotted radish larva powder and plant extracts as active ingredients And to a seasoning prepared thereby.
흰점박이꽃무지(white-spotted flower chafer, Protaetia brevitarsis seulensis)는 딱정벌레목 꽃무지과에 속하는 곤충으로써 한국, 일본, 대만, 중국, 유럽에 분포하며 7월 상순부터 출현하며 출현빈도가 가장 높은 시기는 8월 상순이고, 일부 성충으로 월동하는 개체도 있으나 대부분은 3령 유충으로 월동한다.The white-spotted flower chafer (Protaetia brevitarsis seulensis) is an insect belonging to the Coleoptera brevitarsis family. It is distributed in Korea, Japan, Taiwan, China, and Europe. It appears from early July, and the highest frequency of appearance is 8 It is the beginning of the month and some individuals winter as adult insects, but most of them winter as 3 instar larvae.
알, 유충, 번데기, 성충의 시기를 거치는 완전변태를 하는 곤충이며 현재 대량생산하고 있는 사육농가가 형성되어져 있으며, 딱정벌레목에 속하는 유충을 칭하는 “굼벵이”로 불리기도 한다.It is an insect that undergoes a complete transformation through the period of eggs, larvae, pupa, and adult insects, and breeding farms that are currently mass-producing are formed, and they are sometimes called "slugs" which refer to larvae belonging to the order Beetle.
흰점박이꽃무지 유충은 <동의보감>에서 “간에서 비롯되는 질병, 즉 간암, 간경화, 간염, 누적된 피로의 해소 등을 포함하여 월경불순, 시력감퇴, 백내장, 금창, 산후풍, 악성종기, 구내염, 파상풍, 중풍 등의 성인병을 치료하는데 효과가 있다”라고 보고되고 있다. 따라서 최근 흰점박이꽃무지를 대상으로 기능성 분석 등을 위한 연구가 활발히 진행되고 있다.In <Donguibogam>, the larvae of white-spotted radish are described in "Diseases originating from the liver, namely liver cancer, cirrhosis, hepatitis, and relieving accumulated fatigue. It is reported that it is effective in treating adult diseases such as tetanus and stroke.” Therefore, researches for functional analysis and the like are being actively conducted on the white spotted flower ignorance.
흰점박이꽃무지 유충과 관련된 특허 문헌의 경우는 한국공개특허 제10-2012-0066121호(발명의 명칭 : 흰점박이꽃무지를 유효성분으로 포함하는 염증성 질환의 예방 및 치료용 조성물)에서 흰점박이꽃무지를 유효성분으로 포함하는 염증성 질환의 예방 및 개선용 식품 조성물 및 제조방법에 대해 개시하고 있다.In the case of patent documents related to the white spotted flower radish larva, the white spotted flower in Korean Patent Publication No. 10-2012-0066121 (name of the invention: composition for the prevention and treatment of inflammatory diseases containing white spotted flower radish as an active ingredient). Disclosed is a food composition and a manufacturing method for the prevention and improvement of inflammatory diseases comprising plain green as an active ingredient.
한편, 당뇨병은 그 발생 기점에 따라 제1형 당뇨병(typeldiabetes mellitus)과 제2형 당뇨병으로 분류할 수 있다. 당뇨병환자의 90%정도가 인슐린(insulin) 비의존성 제2형의 당뇨병으로 1형인 인슐린 의존형 당뇨병과는 달리 췌장에서는 인슐린을 분비하지만 분비량이 상대적으로 부족하거나 인슐린작용이 원활하지 않아 발병하며 당질대사 및 지질대사 그리고 단백질대사의 이상으로 인해 급성과 만성합병증의 발생으로 특징지워지는 다양한 원인과 병인을 지닌 일련의 질환군으로 아직까지 근원적으로 치료할 수 있는 약물을 개발하지 못하고 있는 실정이다.On the other hand, diabetes can be classified into type 1 diabetes (typeldiabetes mellitus) and type 2 diabetes according to its origin. About 90% of diabetic patients are insulin-independent type 2 diabetes, and unlike type 1 insulin-dependent diabetes, the pancreas secretes insulin, but the secretion is relatively insufficient or insulin action is not smooth. Due to abnormalities in lipid metabolism and protein metabolism, a series of diseases with various causes and etiologies, characterized by the occurrence of acute and chronic complications, has not yet developed a drug that can fundamentally treat drugs.
흰점박이꽃무지 유충을 함유하는 당뇨질환 개선용 조성물과 관련하여, 한국공개특허 제10-2004-0036992호(발명의 명칭: 흰점박이꽃무지 유충으로부터 조추출물을 추출하는 방법및 그 조추출물을 유효성분으로 함유하는 당뇨질환 치료용약학적 조성물)에서 흰점박이꽃무지 유충을 유효성분으로 함유하는 당뇨질환 치료용약학적 조성물에 대해 개시하고 있다.With regard to the composition for improving diabetic diseases containing white spotted radish larva, Korean Patent Laid-Open Publication No. 10-2004-0036992 (name of invention: method of extracting crude extract from white-spotted radish larva and effective use of the crude extract A pharmaceutical composition for treating diabetic diseases containing as an ingredient) discloses a pharmaceutical composition for treating diabetic diseases containing white spotted radish larva as an active ingredient.
그러나 흰점박이꽃무지 유충 추출물 및 식물 추출물을 모두 포함하면서 보다 개선된 항당뇨 식품 조성물에 대해서는 개시되지 않고 있다.However, a more improved antidiabetic food composition, including both the white spotted radish larva extract and plant extract, has not been disclosed.
따라서 본 발명의 목적은 흰점박이꽃무지 유충 분말 및 식물 추출물을 유효성분으로 함유한 항당뇨 활성 식품 조미료의 제조방법 및 그에 의해 제조된 조미료를 제공하는데 있다.Accordingly, an object of the present invention is to provide a method for preparing an antidiabetic active food seasoning containing white spotted radish larva powder and plant extracts as active ingredients and seasonings prepared thereby.
상술한 목적을 달성하기 위한 본 발명의 실시예에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법은, (A) 흰점박이꽃무지 유충 분말 및 꾸지뽕 열매 추출물을 혼합하여 혼합물을 제조하는 단계; (B) 상기 혼합물을 발효조에 투입하고 유산균으로 발효하여 발효액을 제조하는 단계; (C) 상기 발효액에 에탄올을 혼합하여 에탄올 추출물을 수득하는 단계; 및 (D) 상기 에탄올 추출물을 감압 농축하여 건조한 분말, 흰점박이꽃무지 유충 분말 및 식용 가능한 식재료 분말을 혼합하여 조미료를 제조하는 단계를 포함한다.A method of preparing a seasoning containing white spotted radish larva powder according to an embodiment of the present invention for achieving the above object, (A) preparing a mixture by mixing white spotted radish larva powder and Cudrania fruit extract ; (B) introducing the mixture into a fermentation tank and fermenting it with lactic acid bacteria to prepare a fermentation broth; (C) mixing ethanol to the fermentation broth to obtain an ethanol extract; And (D) concentrating the ethanol extract under reduced pressure to prepare a seasoning by mixing dry powder, white spotted radish larva powder, and edible food ingredient powder.
또한, 본 발명에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법에 있어서, 상기 (C) 단계는, 상기 에탄올 추출물을 헥산, 에틸아세테이트, 부탄올로 순차적으로 분획하여 상기 에탄올 추출물의 에틸아세테이트 분획물을 수득하는 단계를 더 포함하는 것을 특징으로 한다.In addition, in the method for producing a seasoning containing the white spotted radish larva powder according to the present invention, the step (C) comprises sequentially fractionating the ethanol extract into hexane, ethyl acetate, and butanol, and ethyl acetate of the ethanol extract. It characterized in that it further comprises the step of obtaining a fraction.
또한, 본 발명에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법에 있어서, 상기 (A) 단계는, 상기 혼합물이 자작나무 껍질 추출물을 더 포함하는 것을 특징으로 한다.In addition, in the method for producing a seasoning containing white spotted radish larva powder according to the present invention, the step (A) is characterized in that the mixture further comprises a birch bark extract.
본 발명의 실시예에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료는 상기 어느 하나의 제조방법으로 제조될 수 있다.The seasoning containing the white spotted flower radish larva powder according to an embodiment of the present invention may be prepared by any one of the above manufacturing methods.
본 발명에 따르면, 당뇨 유발 동물에서 혈당을 현저히 강하시킬 수 있어 당뇨개선용의 건강식품 및 조미료에 유용하게 활용할 수 있다.According to the present invention, it is possible to significantly lower blood sugar in diabetic animals, and thus can be usefully used in health foods and seasonings for improving diabetes.
도 1은 본 발명의 일 실시예에 따른 제조공정에 대한 흐름도이다.1 is a flowchart of a manufacturing process according to an embodiment of the present invention.
이하, 첨부된 도면을 참조하여 본 명세서에 개시된 실시 예를 상세히 설명하되, 도면 부호에 관계없이 동일하거나 유사한 구성요소는 동일한 참조 번호를 부여하고 이에 대한 중복되는 설명은 생략하기로 한다. 이하의 설명에서 사용되는 구성요소에 대한 접미사 "모듈" 및 "부"는 명세서 작성의 용이함만이 고려되어 부여되거나 혼용되는 것으로서, 그 자체로 서로 구별되는 의미 또는 역할을 갖는 것은 아니다. 또한, 본 명세서에 개시된 실시 예를 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 명세서에 개시된 실시 예의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다. 또한, 첨부된 도면은 본 명세서에 개시된 실시 예를 쉽게 이해할 수 있도록 하기 위한 것일 뿐, 첨부된 도면에 의해 본 명세서에 개시된 기술적 사상이 제한되지 않으며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변경, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. Hereinafter, exemplary embodiments disclosed in the present specification will be described in detail with reference to the accompanying drawings, but identical or similar elements are denoted by the same reference numerals regardless of reference numerals, and redundant descriptions thereof will be omitted. The suffixes "module" and "unit" for components used in the following description are given or used interchangeably in consideration of only the ease of preparation of the specification, and do not have meanings or roles that are distinguished from each other by themselves. In addition, in describing the embodiments disclosed in the present specification, when it is determined that a detailed description of related known technologies may obscure the subject matter of the embodiments disclosed in the present specification, the detailed description thereof will be omitted. In addition, the accompanying drawings are for easy understanding of the embodiments disclosed in the present specification, and the technical idea disclosed in the present specification is not limited by the accompanying drawings, and all modifications included in the spirit and scope of the present invention It should be understood to include equivalents or substitutes.
제1, 제2 등과 같이 서수를 포함하는 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 상기 구성요소들은 상기 용어들에 의해 한정되지는 않는다. 상기 용어들은 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.Terms including ordinal numbers, such as first and second, may be used to describe various elements, but the elements are not limited by the terms. These terms are used only for the purpose of distinguishing one component from another component.
어떤 구성요소가 다른 구성요소에 "연결되어" 있다거나 "접속되어" 있다고 언급된 때에는, 그 다른 구성요소에 직접적으로 연결되어 있거나 또는 접속되어 있을 수도 있지만, 중간에 다른 구성요소가 존재할 수도 있다고 이해되어야 할 것이다. 반면에, 어떤 구성요소가 다른 구성요소에 "직접 연결되어" 있다거나 "직접 접속되어" 있다고 언급된 때에는, 중간에 다른 구성요소가 존재하지 않는 것으로 이해되어야 할 것이다.When a component is referred to as being "connected" or "connected" to another component, it is understood that it may be directly connected or connected to the other component, but other components may exist in the middle. Should be. On the other hand, when a component is referred to as being "directly connected" or "directly connected" to another component, it should be understood that there is no other component in the middle.
단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. Singular expressions include plural expressions unless the context clearly indicates otherwise.
본 출원에서, "포함한다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.In the present application, terms such as "comprises" or "have" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or numbers, steps, actions, components, parts, or combinations thereof, does not preclude in advance.
이하, 도면들을 참조하여 본 발명의 실시예에 대해 상세히 설명하기로 한다. 본 발명은 본 발명의 정신 및 필수적 특징을 벗어나지 않는 범위에서 다른 특정한 형태로 구체화될 수 있음은 당업자에게 자명하다Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the drawings. It is obvious to those skilled in the art that the present invention can be embodied in other specific forms without departing from the spirit and essential features of the present invention.
도 1은 본 발명의 일 실시예에 따른 제조공정에 대한 흐름도이다.1 is a flowchart of a manufacturing process according to an embodiment of the present invention.
도 1을 참조하면, 본 발명의 실시예에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법은, (A) 흰점박이꽃무지 유충 분말 및 꾸지뽕 열매 추출물을 혼합하여 혼합물을 제조하는 단계; (B) 상기 혼합물을 발효조에 투입하고 유산균으로 발효하여 발효액을 제조하는 단계; (C) 상기 발효액에 에탄올을 혼합하여 에탄올 추출물을 수득하는 단계; 및 (D) 상기 에탄올 추출물을 감압 농축하여 건조한 분말, 흰점박이꽃무지 유충 분말 및 식용 가능한 식재료 분말을 혼합하여 조미료를 제조하는 단계를 포함한다.Referring to FIG. 1, a method for preparing a seasoning containing white spotted radish larva powder according to an embodiment of the present invention includes: (A) preparing a mixture by mixing white spotted radish larvae powder and Cudrania fruit extract; (B) introducing the mixture into a fermentation tank and fermenting it with lactic acid bacteria to prepare a fermentation broth; (C) mixing ethanol to the fermentation broth to obtain an ethanol extract; And (D) concentrating the ethanol extract under reduced pressure to prepare a seasoning by mixing dry powder, white spotted radish larva powder, and edible food ingredient powder.
본 명세서에서 사용하는 용어‘흰점박이꽃무지 유충’은 딱정벌레목 꽃무지과에 속하는 곤충의 유충을 의미할 수 있다.As used herein, the term "white spotted flower larvae" may refer to the larvae of insects belonging to the Coleoptera family.
본 발명의 바람직한 양태에 따르면, 본 발명의 상기 흰점박이꽃무지 유충은 바람직하게는 세척된 흰점박이꽃무지 유충일 수 있고, 보다 바람직하게는 세척 및 멸균된 흰점박이꽃무지 유충일 수 있으며, 가장 바람직하게는 세척, 멸균 및 건조된 흰점박이꽃무지 유충일 수 있다.According to a preferred embodiment of the present invention, the white-spotted flowery larvae of the present invention may preferably be a washed white-spotted flowery larvae, more preferably, a washed and sterilized white-spotted flowery larvae, most preferably May be washed, sterilized and dried white spotted radish larva.
꾸지뽕나무((Cudrania tricuspidata (Carr.) Bureau ex)은 뽕나무과에 속하는 낙엽교목으로 원산지는 한국이며, 황해도 이남에서 자라는 낙엽소교목 또는 관목으로서 가지에 가시가 있고, 잔가지에 털이 있다. 열매는 취과로 둥글며, 지름 2.5cm 정도로서 육질이고, 9 ∼ 10월에 적색으로 성숙하며, 수과는 길이 5mm정도로서 흑색이다. 과육은 달고 식용가능하다. 잎은 습진, 유행성이하선염, 폐결핵, 급성관절염 등의 한방치료에 사용하고 있으며, 민간에서는 열매와 수피를 강장, 중풍, 이뇨, 진해 등의 치료약으로 이용하고 있다. 일반적으로 꾸지뽕나무 열매의 성분특성은 수분이 58.0%, pH 6.4, 당도 16.3 Brix이다.Cudrania tricuspidata (Carr.) Bureau ex) is a deciduous arboreous tree belonging to the Moraceae family. Its origin is Korea. It is a deciduous small arboreous tree or shrub growing in the southern part of Hwanghae-do, with thorns on branches and hairs on twigs. Round, 2.5cm in diameter, fleshy, mature in red from September to October, and the achene is 5mm long, black, and the flesh is sweet and edible, and the leaves are used for the treatment of eczema, mumps, pulmonary tuberculosis, acute arthritis, etc. In the private sector, fruit and bark are used as therapeutic drugs for tonic, stroke, diuretic, and jinhae, etc. In general, the component properties of Cudrania fruit are 58.0% moisture, 6.4 pH, and 16.3 Brix sugar.
상기 (B)단계의 상기 혼합물을 발효조에 투입하고 유산균으로 발효하여 발효액을 제조하는 단계에 있어서, 상기 혼합물은 유산균으로 발효시킬 수 있는데, 이때 사용가능한 유산균은 상기 혼합물을 발효시킬 수 있는 것으로 특별히 한정된 것은 아니나, 바람직하게는 락토바실러스 람노서스 KCTC 13088 (Lactobacillus rhamnosus KCTC 13088)일 수 있다. 상기 종균을 1% (v/v) 접종하여 37℃에서 48시간 동안 호기환경에서 정치배양하여 발효시키는 것이 바람직하다.In the step of preparing a fermentation broth by putting the mixture of step (B) into a fermentation tank and fermenting it with lactic acid bacteria, the mixture can be fermented with lactic acid bacteria, and the lactic acid bacteria that can be used are specifically limited to those capable of fermenting the mixture. Although not, it may be preferably Lactobacillus rhamnosus KCTC 13088 (Lactobacillus rhamnosus KCTC 13088). It is preferable to inoculate the seed with 1% (v/v) and cultivate it in an aerobic environment for 48 hours at 37°C for fermentation.
상기 (C) 단계는, 상기 에탄올 추출물을 헥산, 에틸아세테이트, 부탄올로 순차적으로 분획하여 에탄올의 에틸아세테이트 분획물을 수득하는 단계를 더 포함할 수 있다.The step (C) may further include the step of sequentially fractionating the ethanol extract with hexane, ethyl acetate, and butanol to obtain an ethyl acetate fraction of ethanol.
본 발명에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법에 있어서, 상기 (A) 단계는, 상기 혼합물이 자작나무 껍질 추출물을 더 포함하는 것을 특징으로 한다.In the method for producing a seasoning containing white spotted radish larva powder according to the present invention, the step (A) is characterized in that the mixture further comprises a birch bark extract.
자작나무는 쌍떡잎식물 참나무목 자작나무과의 낙엽교목으로 순백색의 수피를 갖고 있으며, 수평으로 종이장처럼 벗겨진다. 수피(樹皮)는 화피(樺皮), 수액(樹液)은 화수액(樺樹液)이라 하며 약용한다. 본 발명에서 사용하는 자작나무 추출물은 껍질의 추출물을 이용할 수 있다. 상기 추출물의 수득을 위해 자작나무의 껍질을 잘 세정하여 건조시키고, 이를 분쇄물로 세단한 것 즉, 원료를 물에 넣고 가열하여 열수 추출하거나, 친수성 유기용매, 함수성 유기용매 또는 친유성 유기용매 중에서 용매추출할 수 있다.Birch is a deciduous tree of the dicotyledonous oak tree birch family. It has a pure white bark and peels horizontally like a sheet of paper. The bark (樹皮) is called Hwapi (樺皮), and the sap (樹液) is called Hwasuak (樺樹液). The birch extract used in the present invention may be an extract of bark. In order to obtain the extract, the bark of birch is well washed and dried, and then cut into a pulverized product, that is, the raw material is put in water and heated to extract hot water, or a hydrophilic organic solvent, a water-soluble organic solvent, or a lipophilic organic solvent The solvent can be extracted from.
본 발명의 실시예에 따른 흰점박이꽃무지 유충 분말을 함유하는 조미료는 상기 어느 하나의 제조방법으로 제조될 수 있다.The seasoning containing the white spotted flower radish larva powder according to an embodiment of the present invention may be prepared by any one of the above manufacturing methods.
본 발명에 따르면, 당뇨 유발 동물에서 혈당을 현저히 강하시킬 수 있어 당뇨개선용의 건강식품 및 조미료에 유용하게 활용할 수 있다. 특히 본 발명의 에탄올 추출물을 헥산, 에틸아세테이트, 부탄올로 순차적으로 분획하여 수득된 에탄올추출물의 에틸아세테이트 분획물의 혈당감소 효과가 우수함을 확인하였다.According to the present invention, it is possible to significantly lower blood sugar in diabetic animals, and thus can be usefully used in health foods and seasonings for improving diabetes. In particular, it was confirmed that the ethyl acetate fraction of the ethanol extract obtained by sequentially fractionating the ethanol extract of the present invention with hexane, ethyl acetate, and butanol has excellent blood sugar reduction effect.
이하, 실시예를 통하여 본 발명을 더욱 상세하게 설명하고자 하나, 하기의 실시예는 단지 설명의 목적을 위한 것이며, 본 발명의 범위를 한정하고자 하는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
< 실시예 1><Example 1>
전문 사육농가에서 3령의 흰점박이꽃무지의 유충을 구입하여 3일간 금식시켜 배설물을 완전히 배출시킨 생것 상태의 유충을 흐르는 물로 3회 수세하였다. 이 후, 유충을 증숙기에 넣은 다음 이에 3배 중량의 정제수를 첨가하여 압력 1.1~1.5kgf/cm2 및 온도 110℃~150℃의 가압고온 조건에서 90분간 가열한 다음, 습식분쇄기(LSI, NEYZSCH, Srlb Bavaria, Germany)를 이용하여 bead size 0.4 mm, rotor speed 3,000 rpm의 조건으로 이 액상을 분쇄하여 유충에 대해 분쇄물을 제조하였다. 상기 분쇄물을 동결 건조하여 흰점박이꽃무지의 유충 분말을 제조하였다.The larvae of the white-spotted radish of 3 instars were purchased from a specialized breeding farm and fasted for 3 days to completely drain the excrement, and the larvae in the raw state were washed 3 times with running water. After that, put the larva in a steamer, and then add 3 times the weight of purified water to it, heat it for 90 minutes at a pressure of 1.1 to 1.5 kgf/cm 2 and a pressurized high temperature of 110 to 150 °C, and then a wet grinder (LSI, NEYZSCH , Srlb Bavaria, Germany) to prepare a pulverized product for larvae by pulverizing this liquid phase under conditions of a bead size of 0.4 mm and a rotor speed of 3,000 rpm. The pulverized material was freeze-dried to prepare white spotted radish larva powder.
꾸지뽕 열매를 경동시장(서울, 제기동)에서 구입하여 추출원료로 사용하였다. 꾸지뽕 열매에 10배 중량의 증류수를 넣고 90℃에서 12시간 추출 후 여과하여 꾸지뽕 열매 추출물을 제조하였다.Cudrania fruit was purchased at Gyeongdong Market (Seoul, Jegi-dong) and used as an extraction raw material. 10 times the weight of distilled water was added to the Cudrania fruit, extracted for 12 hours at 90°C, and filtered to prepare a Cudrania fruit extract.
흰점박이꽃무지 유충 분말 100 중량부에 꾸지뽕 열매 추출물 100 중량부를 혼합하여 혼합물을 제조하였다.A mixture was prepared by mixing 100 parts by weight of Cudrania fruit extract 100 parts by weight of white spotted radish larva powder.
상기 혼합물을 발효조에 투입하고 투입량의 3배 중량의 정제수를 투입하였다. 포도당을 4g/ℓ, 소이펩톤을 3g/ℓ, 효모추출물을 2g/ℓ의 농도로 첨가하고, 사과산을 0.2g/ℓ의 농도로 첨가하여 pH를 5.0으로 조절하였다. The mixture was put into a fermentation tank, and purified water having a weight of 3 times the input amount was added. The pH was adjusted to 5.0 by adding glucose at a concentration of 4 g/l, soypeptone at 3 g/l, and yeast extract at a concentration of 2 g/l, and malic acid at a concentration of 0.2 g/l.
락토바실러스 람노서스 KCTC 13088 (Lactobacillus rhamnosus KCTC 13088) 종균을 1% (v/v) 접종하여 37℃에서 48시간 동안 호기환경에서 정치배양하여 발효시켰다. 발효가 완료된 후 발효액을 120℃에서 15분 동안 살균처리하였다.Lactobacillus rhamnosus KCTC 13088 (Lactobacillus rhamnosus KCTC 13088) seeds were inoculated with 1% (v/v), and cultured in an aerobic environment at 37° C. for 48 hours to ferment. After fermentation was completed, the fermentation broth was sterilized at 120° C. for 15 minutes.
상기 발효액의 10배량의 에탄올(95%)을 가하여 25℃에서 24시간 동안 교반하여 추출하였다. 이후 에탄올 추출물을 증류수에 현탁한 후, 1) 헥센, 2) 에틸아세테이트, 3) 부탄올로 순차적으로 분획하고, 최종적인 4) 물 잔류물을 얻었으며, 이를 감압농축하여 각각을 분말화하였다.10 times the amount of ethanol (95%) of the fermentation broth was added, followed by stirring at 25° C. for 24 hours to extract. After the ethanol extract was suspended in distilled water, 1) hexene, 2) ethyl acetate, and 3) butanol were sequentially fractionated, and a final 4) water residue was obtained, which was concentrated under reduced pressure to powder each.
상기 1) 내지 4)의 분획물에 대한 각각의 분말에 흰점박이꽃무지 유충 분말 및 호박, 양배추, 당근으로 구성된 식용 가능한 식재료 분말(전체 중량의 60%)을 혼합하여 조미료를 제조하였다(실시예 1-1 내지 실시예 1-4).Seasoning was prepared by mixing white spotted radish larva powder and edible food ingredient powder consisting of pumpkin, cabbage, and carrot (60% of the total weight) in each powder for the fractions 1) to 4) above (Example 1 -1 to Example 1-4).
< 실시예 2><Example 2>
실시예 1과 동일하게 제조하되, 흰점박이꽃무지 유충 분말 100 중량부 및 꾸지뽕 열매 추출물 100 중량부를 포함하는 혼합물에 자작나무 껍질을 100℃에서 8시간 열수추출한 열수 추출물 100 중량부를 더 포함시켜 제조하였으며, 상기 실시예 1의 발효조에 투입되는 혼합물의 양과 동일한 양을 발효조에 투입하였다. Prepared in the same manner as in Example 1, but prepared by further including 100 parts by weight of a hot water extract obtained by hot water extraction of birch bark at 100° C. for 8 hours in a mixture containing 100 parts by weight of white spotted radish larva powder and 100 parts by weight of Cudrania fruit extract. , An amount equal to the amount of the mixture added to the fermentor of Example 1 was added to the fermentor.
이후, 발효액의 10배량의 에탄올(95%)을 가하여 25℃에서 24시간 동안 교반하여 추출하였으며, 에탄올 추출물을 증류수에 현탁한 후, 1) 헥센, 2) 에틸아세테이트, 3) 부탄올로 순차적으로 분획하고, 최종적인 4) 물 잔류물을 얻었으며, 이를 감압농축하여 각각을 분말화하였다.Thereafter, 10 times the amount of ethanol (95%) of the fermentation broth was added and extracted by stirring at 25° C. for 24 hours, and the ethanol extract was suspended in distilled water, and then fractionated sequentially with 1) hexene, 2) ethyl acetate, 3) butanol. And, the final 4) water residue was obtained, which was concentrated under reduced pressure to powder each.
상기 1) 내지 4)의 분획물에 대한 각각의 분말에 흰점박이꽃무지 유충 분말 및 호박, 양배추, 당근으로 구성된 식용 가능한 식재료 분말(전체 중량의 60%)을 혼합하여 조미료를 제조하였다(실시예 2-1 내지 실시예 2-4).Seasoning was prepared by mixing white spotted radish larva powder and edible food ingredient powder (60% of the total weight) consisting of pumpkin, cabbage, and carrots in each powder for the fractions 1) to 4) above (Example 2 -1 to Example 2-4).
< 비교예><Comparative Example>
실시예 1과 동일하게 제조하되, 실시예 1의 혼합물 대신 흰점박이꽃무지 유충 분말(비교예 1), 꾸지뽕 열매 추출물(비교예 2), 자작나무 껍질 추출물(비교예 3)을 단독으로 상기 실시예 1의 발효조에 투입되는 혼합물의 양과 동일한 양을 발효조에 투입하였다.Prepared in the same manner as in Example 1, but instead of the mixture of Example 1, white spotted radish larva powder (Comparative Example 1), Cudrania fruit extract (Comparative Example 2), birch bark extract (Comparative Example 3) alone was carried out above The same amount as the amount of the mixture added to the fermentor of Example 1 was added to the fermentor.
< 실험예><Experimental Example>
실험예 1: 실험식이의 조제Experimental Example 1: Preparation of an experimental diet
실험군은 당뇨대조군, 실시예 1-1 내지 1-4, 실시예 2-1 내지 2-4, 비교예 1 내지 3을 대상으로 하였으며, 실험식이는 로덴트(Rodent) AIN-76 식이(Teklad, USA)를 기본으로 하여 조제하였다. 실험동물은 5주령의 평균체중이 140±10 g인 스프라귀-다우리(Sprague-Dawly)계 SPF/VAF 아웃브레드 래트 (outbred rats; Orient Ltd., Sungnamsi, Korea)를 1주일간 환경에 적응시킨 후 각각의 실험군으로 구분하였다.The experimental group was a diabetes control group, Examples 1-1 to 1-4, Examples 2-1 to 2-4, and Comparative Examples 1 to 3, and the experimental diet was Rodent AIN-76 diet (Teklad, USA) was prepared on the basis of. The experimental animals were Sprague-Dawly SPF/VAF outbred rats (Orient Ltd., Sungnamsi, Korea), which had an average weight of 140±10 g at 5 weeks of age, which were adapted to the environment for 1 week. After that, it was divided into each experimental group.
실험군에 대한 실험식이에 있어서, 조미료의 첨가량은 기본식이에 1%(w/w)를 첨가하였다. 실험동물의 수는 실험군당 각각 5마리씩으로 하여 6주간 사육하였다. In the experimental diet for the experimental group, 1% (w/w) was added to the basic diet as the amount of seasoning added. The number of experimental animals was 5 per experimental group and reared for 6 weeks.
실험예 2 : 당뇨유발Experimental Example 2: Induction of diabetes
스트렙토조토신(Streptozotocin; STZ, Sigma Chem. Co. Mo, USA)을 0.1 M 시트르산염 완충액(pH 4.3)에 녹여 체중 250 g이상인 실험동물을 대상으로 55 mg/kg 농도로 대퇴부 근육에 주사하고 STZ 투여 48시간 후 공복 시에 꼬리정맥으로부터 취한 혈액의 혈당농도가 300 mg/dL 이상인 동물을 실험에 이용한다.Streptozotocin (STZ, Sigma Chem. Co. Mo, USA) was dissolved in 0.1 M citrate buffer (pH 4.3) and injected into the thigh muscle at a concentration of 55 mg/kg in an experimental animal weighing more than 250 g. Animals with a blood glucose concentration of 300 mg/dL or more in blood taken from the tail vein on an empty stomach 48 hours after administration are used in the experiment.
실험예 3 : 혈당 분석Experimental Example 3: Blood glucose analysis
실험식이 투여로 인한 혈당감소효과를 조사하기 위하여 실험군당 5마리의 흰쥐를 사용하여 6주간 사육한 후 혈당함량을 비교하였다(표 1). 실험 동물의 혈당은 매일 오전 10~12시에 꼬리부위의 혈관에서 란셋으로 채혈하여 글루코테스타(Gluco- Tester: Life Scan lnc., USA)를 이용하여 측정하였다.In order to investigate the blood sugar reduction effect due to administration of the experimental diet, 5 rats per experimental group were reared for 6 weeks and then the blood sugar content was compared (Table 1). Blood glucose of the experimental animals was measured daily at 10 am to 12 am using a lancet from a blood vessel in the tail area and measured using Gluco-Tester (Life Scan lnc., USA).
당뇨대조군(DM)은 당뇨유발 후 6주간 기본식이만을 급여한 실험군으로 0주째 혈당은 461 mg/dL이었으며 6주째는 432 mg/dL로 6%가 감소하였다.Diabetes control group (DM) was an experimental group that was fed only the basic diet for 6 weeks after diabetes induction. The blood sugar at week 0 was 461 mg/dL, and at week 6, the blood sugar decreased by 6% to 432 mg/dL.
실시예 1-1(헥센 분획물)의 실험군은 0주째 혈당은 483 mg/dL이었으며 6주째는 215 mg/dL로 55%가 감소하였으며, 실시예 1-2(에틸아세테이트 분획물)는 0주째 혈당은 475 mg/dL이었으며 6주째는 138 mg/dL로 71%가 감소하였으며, 실시예 1-3(부탄올 분획물)은 455mg/dL에서 283 mg/dL로 38%가 감소하였고, 실시예 1-4(물 잔류물)는 479mg/dL에서 291mg/dL으로 39% 감소하였다. 반면, 비교예 1은 451mg/dL에서 305mg/dL으로 32% 감소, 비교예 2는 472mg/dL에서 301mg/dL으로 36% 감소, 비교예 3은 487mg/dL에서 366mg/dL으로 25% 감소하였다. 이 같은 결과는 실시예 1-2(에틸아세테이트 분획물)의 혈당감소 효과가 높음을 나타내었다. In the experimental group of Example 1-1 (hexene fraction), the blood sugar at week 0 was 483 mg/dL, and at week 6, the blood sugar was reduced by 55% to 215 mg/dL, and in Example 1-2 (ethyl acetate fraction), the blood sugar at week 0 was It was 475 mg/dL, and 71% decreased from 138 mg/dL at 6 weeks, and Example 1-3 (butanol fraction) decreased 38% from 455 mg/dL to 283 mg/dL, and Examples 1-4 ( Water residue) decreased by 39% from 479 mg/dL to 291 mg/dL. On the other hand, Comparative Example 1 decreased by 32% from 451mg/dL to 305mg/dL, Comparative Example 2 decreased by 36% from 472mg/dL to 301mg/dL, and Comparative Example 3 decreased by 25% from 487mg/dL to 366mg/dL. . This result indicated that the blood glucose reducing effect of Example 1-2 (ethyl acetate fraction) was high.
실시예 2-1(헥센 분획물)의 실험군은 0주째 혈당은 497mg/dL이었으며 6주째는 198mg/dL로 60%가 감소하였으며, 실시예 2-2(에틸아세테이트 분획물)는 0주째 혈당은 485mg/dL이었으며 6주째는 106mg/dL로 78%가 감소하였으며, 실시예 2-3(부탄올 분획물)은 491mg/dL에서 277mg/dL로 44%가 감소하였고, 실시예 2-4(물 잔류물)는 459mg/dL에서 280mg/dL으로 39% 감소하였다. 이 같은 결과는 실시예 2-2(에틸아세테이트 분획물)의 혈당감소 효과가 가장 높음을 나타내었다. In the experimental group of Example 2-1 (hexene fraction), the blood glucose at week 0 was 497 mg/dL, and at week 6, the blood sugar was reduced by 60% to 198 mg/dL, and in Example 2-2 (ethyl acetate fraction), the blood glucose at week 0 was 485 mg/dL. dL, and a decrease of 78% from 6 weeks to 106 mg/dL, Example 2-3 (butanol fraction) decreased by 44% from 491 mg/dL to 277 mg/dL, and Example 2-4 (water residue) It decreased by 39% from 459mg/dL to 280mg/dL. These results showed that Example 2-2 (ethyl acetate fraction) had the highest blood sugar reduction effect.
한편, 이상의 상세한 설명은 모든 면에서 제한적으로 해석되어서는 아니되고 예시적인 것으로 고려되어야 한다. 본 발명의 범위는 첨부된 청구항의 합리적 해석에 의해 결정되어야 하고, 본 발명의 등가적 범위 내에서의 모든 변경은 본 발명의 범위에 포함된다.On the other hand, the above detailed description should not be construed as restrictive in all respects and should be considered as illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention.
Claims (4)
(B) 상기 혼합물을 발효조에 투입하고 유산균으로 발효하여 발효액을 제조하는 단계;
(C) 상기 발효액에 에탄올을 혼합하여 에탄올 추출물을 수득하는 단계; 및
(D) 상기 에탄올 추출물을 감압 농축하여 건조한 분말, 흰점박이꽃무지 유충 분말 및 식용 가능한 식재료 분말을 혼합하여 조미료를 제조하는 단계;를 포함하고,
상기 (A) 단계에서, 상기 혼합물은 자작나무 껍질 열수 추출물을 더 포함하고,
상기 (B)단계에서, 상기 유산균은 락토바실러스 람노서스이고, 상기 락토바실러스 람노서스로 액체발효하여 발효액을 제조하는 것을 특징으로 하고,
상기 (C) 단계에서, 상기 에탄올 추출물은 상기 에탄올 추출물을 헥산, 에틸아세테이트, 부탄올로 순차적으로 분획하여 수득한 상기 에탄올 추출물의 에틸아세테이트 분획물인 것을 특징으로 하는 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법.
(A) preparing a mixture by mixing white spotted radish larva powder and Cudrania fruit extract;
(B) introducing the mixture into a fermentation tank and fermenting it with lactic acid bacteria to prepare a fermentation broth;
(C) mixing ethanol to the fermentation broth to obtain an ethanol extract; And
(D) preparing a seasoning by concentrating the ethanol extract under reduced pressure and mixing dry powder, white spotted radish larva powder, and edible food ingredient powder; Including,
In the step (A), the mixture further comprises a birch bark hot water extract,
In the step (B), the lactic acid bacteria is Lactobacillus rhamnosus, characterized in that to prepare a fermentation broth by liquid fermentation with the Lactobacillus rhamnosus,
In the step (C), the ethanol extract is a seasoning containing white spotted radish larva powder, characterized in that the ethanol extract is an ethyl acetate fraction of the ethanol extract obtained by sequentially fractionating the ethanol extract with hexane, ethyl acetate, and butanol. Method of manufacturing.
상기 흰점박이꽃무지 유충 분말 ,꾸지뽕 열매 추출물 및 자작나무 열수 추출물의 중량비는 1: 1: 1인 것을 특징으로 하는 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법.
The method of claim 1,
The weight ratio of the white spotted flower radish larvae powder, kujipon fruit extract and birch hot water extract is 1: 1: 1, characterized in that the method for producing a seasoning containing white spotted flower radish larva powder.
상기 자작나무 껍질 열수 추출물은 자작나무 껍질을 100℃에서 8시간 열수추출한 열수 추출물인 것을 특징으로 하는 흰점박이꽃무지 유충 분말을 함유하는 조미료의 제조방법.
The method of claim 1,
The birch bark hot water extract is a method for producing a seasoning containing white spotted radish larva powder, characterized in that the hot water extract of birch bark at 100° C. for 8 hours.
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