KR101842752B1 - Natural liposome comprising colagen and hyaluronic acid, peocess for the preparation thereof, and cosmetic composition comprising the same - Google Patents

Abstract

The present invention provides a natural liposome containing collagen and hyaluronic acid, a method for producing the same, and a cosmetic composition comprising the same.

Description

TECHNICAL FIELD The present invention relates to a natural liposome containing collagen and hyaluronic acid, a method for producing the same, and a cosmetic composition containing the collagen and hyaluronic acid, and a cosmetic composition containing the collagen and the hyaluronic acid.

The present invention relates to a natural liposome containing collagen and hyaluronic acid, a method for producing the same, and a cosmetic composition containing the same. More specifically, the present invention relates to a natural liposome which imparts elasticity to the skin, And a cosmetic composition containing the same.

Decreased elasticity of skin tissue can be divided into internal aging due to the skin itself, ultraviolet rays, stress caused by external environment, and external aging. Stress caused by the external environment not only increases the wrinkles of the skin and reduces the elasticity of the skin but also causes pigmentation and skin barrier damage. Chain cleavage and abnormal cross-linking of collagen and hyaluronic acid, which are components of skin connective tissues, cause wrinkles and accelerate aging by decreasing skin moisture. Therefore, it is very important to maintain the moisture and elasticity of the skin to maintain healthy skin (1-5).

Recent research has shown that peptides derived from protein hydrolysates are being used as potential materials to improve wrinkles, improve moisturization, improve skin elasticity, and exhibit specific skin benefits. Representative materials are collagen peptide and hyaluronic acid (6-8), a polymer compound consisting of N-acetylglucosamine and glucuronic acid. Collagen has attracted attention as a cosmetic composition and medical material because of its excellent affinity with cells and low in vivo rejection reaction. Hyaluronic acid is widely used as a cosmetic composition to supplement the weakened horny layer's moisturizing ability. Skin collagen It is also known to help promote elasticity and moisturizing. However, collagen is disadvantageous in that it is insoluble or has a large molecular size even if it is water-soluble, and hyaluronic acid is also a polymer compound, which is not easy to absorb the skin (9-11).

Since the human skin is exposed to the outside, it protects the living body from the stimulation caused by direct contact with the surrounding environment. In other words, the skin functions as a protective film against harmful substances or harmful effects, but also acts as a barrier against the active substances (12).

For this reason, in order to absorb the effective substance to the skin, it is necessary to pass through a pathway passing through the epidermis and the dermis with a high lipid through the stratum corneum of the skin, which is an absorption pathway of the substance in the skin. Is required. The system should be similar to the lipid components of the skin, for example, liposomes composed of a dual lipid membrane can selectively penetrate the active ingredient into the skin.

Here, the liposome is separated from the outer membrane by a membrane composed of a lipid bilayer as a vesicle produced when the phospholipid is suspended in an aqueous solution, and lipids such as cholesterol glycolipids and membrane proteins can be introduced into the membrane. In addition, liposomes can collect ions, low molecular substances, nucleic acids, proteins, and the like in the contained aqueous solution layer, thereby helping to transport these materials. For example, when liposomes are collected and transported, it is possible to absorb these ingredients without breaking them.

However, despite these merits, liposomes have problems such as unstable formulations and very low collection efficiency. In addition, since the formation of the lipid membrane of the liposome is strengthened, the skin permeability of the capturing material is lowered and the release of the active substance is difficult. In addition, solvents, preservatives, and the like used in the production of liposomes may cause skin irritation. Accordingly, various kinds of liposomes have been studied.

In order to solve these problems, it is necessary to develop cosmetics that are safe for the skin and can be used without side effects even when exposed for a long period of time, which helps improve skin elasticity and skin moisturization, and is easily absorbed into the body, It is required to develop a cosmetic composition that is effective in improving skin.

Korean Patent No. 225314, for example, discloses a liposome-type cosmetic composition containing hyaluronic acid as a moisturizing ingredient. However, the above document does not specifically disclose natural liposomes and specifically discloses the skin absorption effect of natural liposomes.

Korean Patent No. 225314

1. Gilchrest BA. 1990. Skin aging and photoaging. DermatolNurs 2: 79-82. 2. Kang KS, Kim ID, Kwon RH, Heo YY, Oh SH, Kim MA, Jung HJ, Kang HY, Ha BJ. 2007. The evaluation of anti-wrinkle effects in oriental herb extract. J Life Sci 17: 1147-1151. 3. Haratake A, Uchida Y, Schmuth M. UVB-induced alterations in permeability barrier function: Roles for epidermal hyperproliferation and thymocyte-mediated response. J. Invest. Dermatol. 108: 769-775 (1998) 4. Park CS. Skin barrier and beauty foods. Food Sci. Indus. 40: 19-26 (2008) 5. Jiang SJ, Chu AW, Lu ZF, Pan MH, Che DF, Zhou XJ. Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. xp. Dermatol. 16: 985-992 (2007). "Antioxidative and Antioxidative Activities of the Extracts of Rutaceae Extracts." Journal of the Korean Chemical Society 28.4 (2011): 482-490. 6. Moskowit RW. Role of collagen hydrolysate in bone and joint disease. Semin. Arthritis Rheu. 30: 87-99 (2000) 7. Lee SJ. Novel natural products as active material for beauty food. Food Sci. Indus. 40: 10-18 (2008) 8. Jeong Kee Kim, Ji Hae Lee, Mi Sook Yang, Dae Bang Seo, and Sang Jun Lee. Beneficial Effect of Collagen Peptide Supplement on Anti-agingAgainst Photodamage. ENGLISH J. FOOD SCI. TECHNOL. Vol. 41, No. 4, pp. 441 ~ 445 (2009) 9. Haratake A, Uchida Y, Schmuth M. UVB-induced alterations in permeability barrier function: Roles for epidermal hyperproliferation and thymocyte-mediated response. J. Invest. Dermatol. 108: 769-775 (1998) 10. Park CS. Skin barrier and beauty foods. Food Sci. Indus. 40: 19-26 (2008) 11. Jiang SJ, Chu AW, Lu ZF, Pan MH, Che DF, Zhou XJ. Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. Exp. Dermatol. 16: 985-992 (2007) 12. Lim, M. S., Han, S. B., Kwon, S. S ,. Park, M. A., and Park, S. N., 2012. Elastic Liposome Formulation for Transdermal Delivery of Rutin. J. Soc. Cosmet. Sci. 2, 147-154

It is an object of the present invention to provide a natural liposome having a skin elasticity and a skin moisturizing function and being easily absorbed into the body by solving the problems of the prior art, a method for producing the same, and a cosmetic composition containing the same.

One example of the present invention includes a first step of mixing at least one natural emulsifier with a natural-derived solvent and ultrasonication, a second step of adding collagen and hyaluronic acid as a collecting material to the product of the first step, A natural liposome comprising collagen and hyaluronic acid as a liposome constituent material and a capturing material containing at least one natural emulsifier, a natural derived solvent, a natural preservative, and the like.

Another example of the present invention provides a cosmetic composition comprising the natural liposome as an active ingredient, and a humectant containing the natural liposome as an active ingredient.

Another example of the present invention is a method for producing a liposome, comprising: a first step of mixing at least one natural emulsifier with a natural solvent and then ultrasonication at 50 to 80 ° C, adding a natural liposome capturing material to the product of the first step, And a third step of adding a distilled water warmed to 40 to 60 ° C to the product of the second step and a natural preservative and sonicating the liposome to form a liposome.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a natural liposome excellent in the collection efficiency and the skin absorption rate without causing problems such as skin irritation and the like. Accordingly, the cosmetic composition according to the present invention can exert excellent effects on skin elasticity, wrinkle and moisturizing.

1 shows a process for producing a natural liposome according to an example of the present invention.
Fig. 2 shows a graph of skin absorption force of each sample.
Figures 3a, 3b and 3c show the particle distribution of each sample.
Fig. 4 shows the intracellular hyaluronic acid content.
Fig. 5 shows collagen secretion amount of dermal cells.
Fig. 6 is a back skin photograph when each sample is applied, and Fig. 7 shows a skin skin thickness measurement result by ultraviolet irradiation.
Fig. 8 shows the content of hyaluronic acid in the skin when irradiated with ultraviolet rays, and Fig. 9 shows the results of skin elasticity evaluation.
Fig. 10 shows a histological evaluation result in the case of irradiation with ultraviolet rays.

Hereinafter, a natural liposome according to the present invention will be described in detail with reference to the accompanying drawings.

However, these descriptions are provided only to illustrate the present invention, and the scope of the present invention is not limited by these exemplary explanations.

1. Natural Emulsifier

Natural emulsifiers are substances that constitute the lipid bilayer and emulsifiers derived from natural materials. The natural emulsifiers include phospholipids and fatty acids. The phospholipids contained in the natural emulsifiers are natural phospholipids derived from natural origin, and fatty acids are natural fatty acids derived from natural sources.

Examples of the phospholipid include phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and their hydrogenation products (for example, phosphatidylserine, phosphatidylglycerol, , And hydrogenated phophatidylcholine (derived from soybean)) may be used.

Examples of the fatty acid include at least one natural fatty acid selected from the group consisting of stearic acid, palmitic acid, oleic acid, linoleic acid, and linolenic acid (derived from soybean) Can be used.

The natural emulsifier may comprise 70 to 90% by weight of a natural phospholipid and 10 to 30% by weight of a natural fatty acid. Other solvent may be contained in the remaining amount. As the solvent, a natural solvent such as distilled water, glycerin derived from natural origin, natural ethanol, natural propanediol, natural glycerin, fermented alcohol can be used, preferably distilled water, natural derived glycerin, natural ethanol, Distilled water can be used.

Natural emulsifiers can be used in combination of two or more kinds. For example, two kinds of natural emulsifiers or three kinds of natural emulsifiers may be mixed and used. When two natural emulsifiers are mixed and used, for example, the first natural emulsifier may be selected from the group consisting of phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, palmitic acid, stearic acid stearic acid, oleic acid, linoleic acid and linolenic acid (derived from soybean), and the second natural emulsifier may be hydrogenated phosphatidylcholine (derived from soybean) . In this case, the first emulsifier component may be contained in an amount of 1 to 5 wt%, and the second emulsifier component may be contained in an amount of 0.5 to 2 wt% based on the total content of the natural liposome. In addition, when three natural emulsifiers are mixed and used, for example, in addition to the first natural emulsifier and the second natural emulsifier, a third natural emulsifier such as cetearylolivate, sorbitanolivate- Olive) can be used.

The natural emulsifier may comprise 0.5 to 17% by weight, preferably 1.5 to 7% by weight, based on the total weight of the natural liposome.

2. Collecting material

Acacia-derived vegetable collagen and microorganism-derived hyaluronic acid can be used as the capturing material that can be collected in the phospholipid layer of the liposome. Further, a mixture obtained by mixing the above components at a certain ratio may be used. The capturing material is contained in an amount of 0.1 to 10% by weight based on the total amount of the natural liposome.

3. Solvent

As the solvent, a natural solvent such as distilled water, glycerin derived from natural origin, natural ethanol, natural propanediol, natural glycerin, fermented alcohol can be used, preferably distilled water, natural derived glycerin, natural ethanol, Distilled water can be used. The content of the solvent is the residual amount of the emulsifier, the trapping agent, and the preservative.

4. Preservatives

As a preservative, one or more natural extracts selected from the group consisting of a superfine fruit extract, a flower leaf extract, and an Earther extract may be used. It is distinguished from benzoic acid, a paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol and the like, which is a commonly used preservative in that it is a natural extract. The preservative may generally be included in an amount of 0.1 to 5% by weight.

5. Production of natural liposomes containing collagen and hyaluronic acid

The liposome according to one embodiment of the present invention can be produced by using ultrasonic waves.

One or more natural emulsifiers are mixed with a part of a natural solvent and homogenized by ultrasonic disruption at 50 to 80 ° C using an ultrasonic liquid processor. And the ultrasonic treatment is performed after heating. When the natural emulsifier is homogenized, collagen and hyaluronic acid, which are the capturing substances, are added, followed by ultrasonic disruption and mixing. Homogenization of the natural emulsifier and the capturing material can be enhanced and the particle size can be controlled by performing the ultrasonic disintegration process in the step of homogenizing the natural emulsifier and the step of adding the collecting material, unlike the prior art using the homogenizer and the like. When the natural emulsifier and the capturing material are completely homogenized, the remaining distilled water heated to 40 ~ 60 ° C and the natural extract (preservative) are added and mixed by ultrasonic disruption to form liposome type particles. Thereafter, the temperature is gradually lowered and ultrasonication is finally performed for 5 minutes to make the particles smaller and uniform so that a natural liposome is finally produced. The manufacturing process is shown in Fig.

The natural liposome according to an embodiment of the present invention is distinguished from conventional synthetic liposome in that it does not contain a synthetic component including a natural emulsifier, a natural solvent, a natural preservative, and a naturally occurring capturing material.

In addition, the natural liposome according to one embodiment of the present invention can improve the absorption rate by increasing the skin permeability, so that the active ingredient can effectively act on the skin. Accordingly, the natural liposome according to one embodiment of the present invention can be effectively used as a cosmetic composition. For example, it may be used in a cosmetic composition in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a cleansing oil, a foundation, More specifically, softening longevity, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, body essence , A makeup base, a foundation, a hair dye, a shampoo, a rinse, a body cleansing agent, and a mask sheet.

≪ Example 1 >

The liposomes were prepared according to the composition shown in Table 1 below.

Raw material Example 1
(Unit: wt%) Natural emulsifier Emulsifier 1 3.0 Emulsifier 2 1.0 Natural preservative One Solvent Distilled water (D.I.Water) Balance Capture material Collagen powder 0.5 Hyaluronic acid 5.0

Emulsifier 1 Emulsifier 1 Emulsifier 1 A mixture of 67 wt% of phosphatidylcholine, 8 wt% of lysophosphatidylcholine, 8 wt% of phosphatidyl ethanolamine, 3 wt% of palmitic acid, 1 wt% of stearic acid, 3 wt% of oleic acid, 7 wt%, linolenic acid 3 wt%

Emulsifier 2: Hydrogenated phophatidylcholine (derived from soy)

Preservative: Pine nuts fruit extract: Waxy pine extract: Earth nere extract is included in weight ratio of 1: 1: 1

The specific manufacturing process is as follows.

First, Emulsifier 1 and Emulsifier 2 were mixed with 1/3 of distilled water and ultrasonicated at 65 ° C using an ultrasonic liquid processor (QSONICA, USA) as shown in Table 1 above. When the emulsifier was homogenized, collagen and hyaluronic acid as a trapping material were added, followed by ultrasonic disruption and mixing. When the emulsifier and the capturing material were completely homogenized, the remaining distilled water (2/3) heated to 50 ° C and the preservative, which is a natural extract, were mixed and ultrasonically disrupted to form liposome-type preliminary particles. Thereafter, the temperature was gradually lowered, and finally ultrasonicated for 5 minutes to make the particles smaller and uniform, thereby finally producing a natural liposome containing collagen and hyaluronic acid. The above manufacturing process is shown in Fig.

≪ Comparative Example 1 &

In Comparative Example 1, instead of the natural liposome of Example 1, collagen and hyaluronic acid used as a capturing material in Example 1 were diluted with distilled water to the same concentration as in Example 1, and used.

≪ Comparative Example 2 &

In Comparative Example 2, a conventional synthetic liposome containing collagen, hyaluronic acid, lecithin, and propylene glycol was prepared. Propylene glycol (10%), which is a synthetic solvent, was used as a solvent, and phospholipids (lecithin-4%) were mixed and heated to 65 캜. When the phospholipid was completely dissolved, collagen and hyaluronic acid were added in the same amount as in Example 1 and homogenized with a homogenizer. The remaining water was mixed with an antiseptic (paraben 0.02%) to form liposome particles, and the particles were homogenized through ultrasonic treatment.

<Experimental Example 1> Confirmation of skin absorption using Franz Diffusion cell

In order to confirm the skin permeation enhancing effect of the collagen and hyaluronic acid natural liposome, the skin absorption capacity of the liposome of Example 1, the mixture of Comparative Example 1 and the liposome of Comparative Example 2 was compared.

The skin permeation experiment was conducted using Franz diffusion cell. In the skin penetration experiment, the degree of absorption was confirmed by using the chicken skin as a skin permeable membrane. The skin tissue was fixed between the donor and the receptor phase, and 1 ml of sample was added to the donor of the prepared Franz diffusion cell and the temperature was maintained at about 32.5 ° C using a constant temperature bath. 0.25 ml of the receptor phase was collected at intervals of 0, 1, 3, 6, 20, and 24 hours with a Pasteur pipette through a sampling port and stored in tubes.

As shown in FIG. 2, the absorbency of the collagen-hyaluronic acid natural liposome of Example 1 was the highest, as a result of comparing the skin absorption tests of the samples of Example 1, Comparative Examples 1 and 2.

<Experimental Example 2> Measurement of collection rate

2 ml of the collagen-hyaluronic acid natural liposome suspension was taken and a 0.45 ㎛ -25 mm syringe filter (GVS, USA) was attached to a 10 ml syringe to remove non-captured liposomal substances. The removed portion was supplemented with distilled water, and the absorbance at the maximum absorption wavelength of collagen and hyaluronic acid was measured to confirm the collection rate. The collection rate of collagen and hyaluronic acid was determined by substituting in the following formula.

Entrapment efficiency (%) = C _e / C _i x 100 (%)

C _e The concentration of the entrapped material (encapsulated concentration)

C _i Initial concentration of all substances

The measured capture rates are shown in Table 2 below.

Sample Entrapment efficiency (%) Example 1 96.11 Comparative Example 2 72.12

As a result of the measurement of the liposomal entrapment ratios of Example 1 and Comparative Example 2, the natural liposomes of Example 1 exhibited a entrapment rate of 96.11% as shown in Table 2, and the entrapment ratio of the synthetic liposome of Comparative Example 2 was 72.12 %.

<Experimental Example 3> Size analysis of liposome by laser particle size analyzer

The particle sizes of the samples of Example 1, Comparative Example 1 and Comparative Example 2 were measured and the particle size distribution was analyzed using a laser particle size analyzer (Electrophoretic Light Scattering Spectrophotometer ELS-8000, Otsuka).

The average particle sizes of the collagen-hyaluronic acid natural liposome of Example 1, the collagen-hyaluronic acid mixture of Comparative Example 1 and the collagen-hyaluronic acid synthetic liposome of Comparative Example 2 are shown in Table 3 below.

Sample Average particle size (nm) Example 1 196.5 Comparative Example 1 674.0 Comparative Example 2 279.5

The particle distribution diagrams of the collagen-hyaluronic acid natural liposome of Example 1, the collagen-hyaluronic acid mixture of Comparative Example 1, and the collagen-hyaluronic acid synthetic liposome of Comparative Example 2 are shown in FIGS. 3A, 3B and 3C, respectively.

[Cell Experiment]

<Experimental Example 4> Comparison of hyaluronic acid production amount in cells

After collagen-hyaluronic acid natural liposome was applied to dermal keratinocyte (HaCaT cell), the cells were recovered and the content of hyaluronic acid present in the cells was measured.

10 10 ⁴ dermal keratinocytes were dispensed into 6 wells, and after 24 hours, the cells were replaced with a fresh medium. Then, the cells were treated with the untreated group, the collagen-hyaluronic acid mixture of Comparative Example 1, the collagen-hyaluronic acid natural liposome of Example 1, 2 collagen-hyaluronic acid synthesis liposome was treated at 1% concentration for 48 hours. After the sample was treated, the cells were recovered and disrupted by sonication to obtain cell lysate. The content of hyaluronic acid was measured by measuring the amount of hyaluronic acid contained in the cell lysate using the Hyaluronan enzyme-linked immunosorbent assay kit (echelon).

The measurement results are shown in Fig. As a result of measuring the content of the hyaluronic acid in the cell lysate, the content of hyaluronic acid in the cell (-) group which was not treated with anything other than the medium was found to be 1401.75 ng / ml in the cell and 1401.94 ng / ml in the case of Comparative Example 1 And it was found to be 1.412 ng / ml in Example 1, which is the highest. On the other hand, in Comparative Example 2, it was slightly lower than that of Example 1 (1411.81 ng / ml).

<Experimental Example 5> Comparison of intracellular collagen production

After applying collagen-hyaluronic acid natural liposome to skin dermal cells (HDF), the content of procollagen present in the cell culture was measured.

24 ^d of skin dermal cells were placed in 24 wells and replaced with fresh medium after 24 hours, respectively. Thereafter, a collagen-hyaluronic acid mixture of Comparative Example 1, collagen-hyaluronic acid natural liposome of Example 1, 2 collagen-hyaluronic acid-containing liposome was treated at a concentration of 1% for 48 hours and the culture solution was recovered. The content of procollagen (collagen precursor) was measured using procollagen type 1 C-peptide (PIP) EIA kit (Takara).

The measurement results are shown in Fig. As a result of measuring the content of procollagen contained in the dermal cell culture medium, it was found that 111.53% of the cells treated with Example 1 had the highest content, and the collagen content of the cells treated with distilled water increased by 10% or more And it was found.

[Animal experiment]

<Experimental Example 6> Effect of skin wrinkles on hairless mice inducing skin aging

1. Experimental animals and aging induction

Six-week-old female hairless mice weighing 25 grams were received from BioLink (Chungcheongbuk-do, Korea) and underwent a treatment period of four weeks after a week of adaptation period. During the test period, skin aging (wrinkle formation) was induced by ultraviolet rays, and 100ul of each sample was applied to the back skin 5 times a week. The incubation room temperature (22 ± 2), relative humidity (55 ± 5%) and intensity were maintained for 12 hours. Ultraviolet light treatment was performed three times a week and treated with the intensity of 1MED in the first week, 2MED in the second week, 3MED in the third week and 4MED in the fourth week of the treatment period.

2. Observation of skin wrinkle by ultraviolet irradiation

UV rays were irradiated to observe skin wrinkles of hairless mice causing skin aging (wrinkles). Hyaluronic acid natural liposome of Example 1, a collagen-hyaluronic acid mixture of Comparative Example 1, and a collagen-hyaluronic acid synthetic liposome of Comparative Example 2 were applied to skin such as hairless mice in each experimental group.

FIG. 6 is a photograph of the back skin of the hairless mice treated with UV (-) treated group and UV (+) treated group, positive control group retinol treated with Example 1, Comparative Example 1 and Comparative Example 2 , And this skin was magnified 50 times and photographed with a microscope. It was confirmed that the occurrence of wrinkles was also reduced in the group treated with the positive control group of retinol and the control group of Comparative Example 1. In the case of the mouse treated with Example 1, it was confirmed that the occurrence of wrinkles was reduced in the skin similar to the back skin of the group not irradiated with ultraviolet rays.

<Experimental Example 7> Skin thickness change by ultraviolet irradiation

When the ultraviolet rays are irradiated, the stratum corneum of the skin becomes thick and the skin thickness increases. The changes of skin thickness according to ultraviolet irradiation were measured, and the thickness of skin such as hairless mice in each experimental group was confirmed. After the completion of the animal experiment, the dorsal skin of hairless mice was recovered and the thickness was measured in mm using a caliper.

The measurement results are shown in Fig. Skin thickness of mice irradiated with ultraviolet light was increased and retinol treated with positive control was similar to skin thickness of hairless mice not irradiated with ultraviolet light. In Example 1, skin thickness was 0.06 mm less than UV (+) (UV) irradiation and 7.2% less than UV (+) treated group.

<Experimental Example 8> Comparison of hyaluronic acid production in skin of hairless mice irradiated with ultraviolet rays

The skin of the collagen-hyaluronic acid natural liposome of Example 1, the collagen-hyaluronic acid mixture of Comparative Example 1, and the collagen-hyaluronic acid synthetic liposome of Comparative Example 2 was recovered and the content of hyaluronic acid in the skin tissue was analyzed.

As a result, as shown in FIG. 8, it was confirmed that the content of hyaluronic acid was decreased by UV (ultraviolet) irradiation, and the content of hyaluronic acid was increased in all the groups treated with the sample. The collagen-hyaluronic acid natural liposome-treated skin tissue was found to contain most of the hyaluronic acid.

Experimental Example 9 Evaluation of skin elasticity of hairless mice irradiated with ultraviolet rays

Skin elasticity of hairless mice irradiated with ultraviolet rays was evaluated using a cutometer (Courage-khazaka Electronic, MPA580). The ultraviolet rays of the skin are less elastic than those of the skin.

The skin elasticity of the mice treated with the samples was measured. The skin elasticity was measured after connecting the elasticity measuring probe. The relative elasticity of the skin was measured by observing the degree of skin deformation (expansion) and contraction caused by sound pressure. It was measured three times continuously with 2 seconds of on-time and 2 seconds of off-time with a constant negative pressure of 450 mbar. The degree of skin deformation and the degree of restoration were measured by gross elasticity. Ua / Uf; R2).

As shown in FIG. 9, the skin elasticity of the skin of Maos treated with the retinol positive control group was increased, but the elasticity of the skin of the mouse treated with Comparative Example 1 was decreased. On the other hand, it was confirmed that the mouse treated with Example 1 showed an increase in elasticity by 20% or more as compared with the group irradiated with UV (+) (ultraviolet ray).

<Experimental Example 10> Histological evaluation of hairless mice irradiated with ultraviolet light

For histological observation of the treated skin, a part of the dorsal skin tissue was fixed in 10% formalin, embedded in paraffin, discarded to a thickness of 4 μm, and stained with masson's trichrome staining method.

The results are shown in Fig. The ultraviolet irradiation showed thickening of the epidermal layer of the skin tissue and a decrease of the collagen of the dermal layer, and the surface of the skin became rough. The skin treated with retinol, a positive control, had a thick epidermal layer and a marked decrease in stained collagen fibers in the dermal layer. The skin of hairless mice treated with Example 1 was similar to the skin not irradiated with ultraviolet rays, and it was confirmed that the thickness of the epidermal layer was thin and the collagen fibers of the dermal layer were not damaged and maintained a healthy state.

Claims (14)

A first step of mixing at least one natural emulsifier with a natural solvent and ultrasonic treatment; a second step of adding collagen and hyaluronic acid as a collecting material to the product of the first step and ultrasonication; A third aspect of the present invention is a natural liposome formed by a method comprising a third step of adding a derived solvent and a natural preservative and sonicating,
One or more natural emulsifiers, a natural derived solvent, a liposome constituent material containing a natural preservative,
Comprising collagen and hyaluronic acid as a collecting material,
Wherein the natural emulsifier comprises at least one natural phospholipid selected from the group consisting of phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylic acid, phosphatidylserine, phosphatidylglycerol, phosphatidylinosylol, and their hydrogenation products, Stearic acid, oleic acid, and linoleic acid,
The natural-derived solvent is distilled water, natural ethanol, natural propanediol, natural glycerin or fermented alcohol,
Wherein the natural preservative is at least one natural-derived extract selected from the group consisting of a superfine fruit extract,
Based on the total weight of the natural liposome, 0.5 to 17% by weight of the natural emulsifier, 0.1 to 5% by weight of the natural preservative, 0.1 to 5% by weight of the absorbent, and the remaining amount of the natural derived solvent. delete delete delete delete The method according to claim 1,
The natural-derived solvent is distilled water. The method according to claim 1,
Wherein said natural emulsifier comprises a first natural emulsifier containing at least one fatty acid and at least one phospholipid and a second natural emulsifier containing at least one phospholipid. 8. The method of claim 7,
Wherein the second natural emulsifier is at least one emulsifier selected from the group consisting of sorbitan olivate and cetearyl olivate. delete The method according to claim 1,
Natural liposome for promoting skin absorption. A cosmetic composition comprising the natural liposome of claim 1 as an active ingredient. A humectant comprising the natural liposome of claim 1 as an active ingredient. A first step of mixing at least one natural emulsifier with a natural-derived solvent and ultrasonication at 50 to 80 캜,
A second step of adding collagen and hyaluronic acid as a collecting material to the product of the first step,
A third step of adding a natural-derived solvent heated to 40 to 60 ° C and a natural preservative to the product of the second step and forming a liposome by ultrasonication
10. A method for producing a natural liposome according to any one of claims 1, 6 to 8 and 10, 14. The method of claim 13,
And a fourth step of performing ultrasonic treatment while gradually lowering the temperature after the third step.

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