KR101835799B1 - A cosmetic composition for manufacturing by the using of propolis, and the cosmetic manufactured by the composition - Google Patents

Abstract

The present invention relates to a cosmetic composition containing propolis as an active ingredient and a cosmetic comprising the composition.
More specifically, it is possible to prevent skin aging or skin diseases by using propolis having an effect of antibacterial, anti-cancer, anti-inflammatory, anti-inflammatory, antioxidant and immunity enhancement as active ingredients, The present invention relates to a cosmetic composition which can increase the efficacy such as antioxidation, antibacterial, anti-aging, skin deflection improvement, immune enhancement and skin disease prevention.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing propolis as an active ingredient and a cosmetic composition containing the composition. [0002]

The present invention relates to a cosmetic composition containing propolis as an active ingredient and a cosmetic comprising the composition.

More specifically, it is possible to prevent skin aging or skin diseases by using propolis having an effect of antibacterial, anti-cancer, anti-inflammatory, anti-inflammatory, antioxidant and immunity enhancement as active ingredients, The present invention relates to a cosmetic composition which can increase the efficacy such as antioxidation, antibacterial, anti-aging, skin deflection improvement, immune enhancement and skin disease prevention.

Cosmetics are collectively referred to as creams, fragrances and perfumes which are used for make-up or cosmetics, and have been used for decorating the skin for the first time, and recently used for beauty and skin care .

As described above, cosmetics which have been manufactured so that the purpose of cosmetics is concentrated on cosmetic and skin care, are recently released as functionalities for managing skin health by the effects of various components, have.

Although a number of conventional cosmetic compositions have appeared in the past, the present applicant intends to provide cosmetic compositions having antibacterial, anti-inflammatory, anti-cancer and antioxidative properties by containing propolis as an active ingredient.

Propolis is a material made by mixing bees with their own needles and enzymes in a resin-like substance extracted from various plants for their survival and reproduction, which will be described later, also known as russian penicillin and natural penicillin.

These propolis contains the following ingredients.

① Flavonoids: Flavonoids, which are the main components of propolis, are known to have major pharmacological effects such as antimicrobial activity, antiinflammatory activity, and antioxidant activity. Major constituents include chlorine, apigenin, acacetin, quercetin, , Pinosembrine, camprifide, pinovansacin, pinostrobin, luteolin, artefilin C, ferulic acid, benzoic acid, gallic acid, cinnamic acid and its derivatives, caffeic acid and its esters.

② Amino acids: Propolis contains a small amount of amino acids. It mainly contains more than 10 kinds of amino acids such as aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine and valine.

③ Mineral and Heavy Metals: Propolis contains trace minerals such as iron, zinc, and manganese. Heavy metals such as arsenic and mercury which are harmful to human body are not detected at all. Lead is not detected or detected below 1ppm, have.

Propolis containing these components has the following effects.

① Antimicrobial anti-inflammatory action: The temperature of the honeycomb in which tens of thousands of bees live is suitable for various bacteria in the vicinity of 34 ℃, but it is always kept sterile due to propolis. Bees prevent the propagation of pathogens by applying propolis to the entrance and inner walls of the beehive. When other organisms invade, they are coated with propolis to prevent corruption. Several studies have shown that Bacillus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Trichomonas spp., Salmonella spp. Are effective against various bacteria, and it is found that propolis suppresses bacterial protein synthesis and plays antimicrobial action.

② Antioxidant activity: 3% of human oxygen is changed into active oxygen in the body. When this active oxygen is overproduced and causes diseases, it is called harmful oxygen, and the oxidizing action of active oxygen makes lipid peroxide. This active oxygen and lipid peroxide is seen as the cause of 80 ~ 90% of diseases such as aging, cancer, arthritis, adult diseases, incurable diseases.

The only defense against the active oxygen in the body is the SOD enzyme. When superoxide dismutase (SOD) enzyme is actively produced and neutralized with active oxygen, active oxygen is produced in our body. However, when a person is aged 40 years or older, the ability to synthesize in the cell drops rapidly, which promotes aging and cell damage.

The substances that can contribute to the decomposition and inhibition of active oxygen by increasing the function of SOD enzyme are contained in greenish-yellow vegetables, seeds, and tree bark. Among them, propolis is the most abundant.

③ Analgesic Anesthetic Action: It is known that the alcohol extract of propolis is much stronger (about 3 times) than the ***e or procaine and acts as an anesthetic in the rabbit's cornea. It also has the same effect as nobokain, and water extract propolis is said to have the same effect. The analgesic effect of propolis is similar to aspirin and has no side effects because it is a natural substance. In addition, it has a good analgesic effect and has an effect of elevating the therapeutic effect on other drugs without imposing a burden on the stomach or the stomach.

④ Immune function: The natural healing power of humans has no immune function. One of the enhancements of immune function is the activation of macrophages.

When the macrophage is treated with propolis, the macrophage increases in its ability to eat bacteria and the like as time passes and the activity becomes more active.

⑤ Antiviral action: Three types of interferon used for cancer treatment are known as α, β and γ. Interferon does not directly attack the virus. Since each cell in the body has a gene that makes interferon based on the possibility of virus invasion, it temporarily protects the virus from infection by changing the metabolic state temporarily and acting only on the cell side.

Viruses, bacteria, and other interferon-inducing substances are called interferon inducers. Flavonoids contained in propolis are also one of these inducers. It is also known that interferon activates the natural antibody NK cells (Natural Killer) that appears even when it does not react with the target cell antigen, and exerts its antiviral action.

Flavonoids in propolis stimulate leukocytes to produce interferons (antiviral substances) in large quantities to protect viruses from entering the cells.

⑥ Anticancer Action: In 1991, Dr. Matsuno Tetsuya of Japan National Institute for Preventive Health and Prevention succeeded in separating three kinds of anticancer substances from Brazilian propolis into pure form. Caffeic acid phenate ester, quercetin, and cloro-glycidyl terpene. Cleo-dandy terpen was the first substance discovered by Dr. Matsuno. This substance has been found to kill cancer cells and its chemical structure has been clearly identified. For reference, the propolis that Dr. used at this time was Brazil and extracted micelles. In addition to these three substances, Dr. Matsuno separated and characterized four substances from propolis. After that, one of the four substances was found to be 'Arthropylline C', which has anticancer effect, Professor Tetsuo Honki of Kawasaki University of Medical Science revealed.

Because of the poor solubility of such propolis, its use in cosmetics has generally been limited to the use of aqueous extracts with propolis extracts.

In other words, propolis has been used as a cosmetic because it has the property of destroying emulsification which is a general formulation of cosmetic due to the poor solubility characteristic of propolis. In recent years, propolis has been used as a cosmetic product through continuous research .

Regarding this, among the technologies filed in 2006, Patent Application No. 10-0817781 discloses a cosmetic composition containing propolis extract extracted with tocopherol as a technique for applying propolis.

The above-described technique is a cosmetic composition comprising, as an active ingredient, an extract obtained by a method for extracting propolis using tocopherol, which comprises the steps of: adding tocopherol to the propolis raw material; 2) mixing and stirring the propolis raw material and tocopherol in the first step to extract an active ingredient; 3) filtering the extract of step 2 to remove impurities; And 4) adding the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition '; and' adding tocopherol to the propolis viscous precipitate. 2) mixing and stirring the viscous sediment and tocopherol of the first step to extract an active ingredient; 3) filtering the extract of step 2 to remove impurities; And 4) containing the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition.

That is, the above-described technique does not contain an additional composition for the functionalization of cosmetics in addition to propolis in the production of cosmetic compositions using propolis.

On the other hand, as a technology filed in 2012, a moisturizing cosmetic composition containing propolis nano vesicle is described in Patent Publication No. 10-1410042 in connection with a technique including an additional composition together with propolis.

The above-described technique relates to a moisturizing cosmetic composition containing propolis nano vesicle prepared by adding a certain amount of propolis-containing nano-vesicle composition, wherein the propolis-containing nano-beacycle is PEG-5 rape seed sterol (PEG 3 to 40 wt% of Ceteth-3, CH 3 (CH 2) 14 CH 2 (OCH 2 CH 2) nOH), 12 to 36 wt% of cholesterol, and potassium cetyl phosphate 1 to 20% by weight of propolis, and 1 to 20% by weight of propolis is dissolved completely at 70 to 75 ° C to prepare a first composition (S10); and 1 to 20% by weight of propolis, hydrogenated lecithin 20 to 28 wt% of a pre-composition of 100 wt% composed of a mixture of 20 to 20 wt% of 1,3-butylene glycols and 75 to 90 wt% of 1,3-butylene glycols; 0.01 to 0.1 wt% of Ethylenediaminetetraacetic Acid (EDTA-2Na); 3 to 10 wt% of glycerine; 1 to 6 wt% of propylene glycol; (Step S20) of completely dissolving DI-Water at 60 to 75 wt% at 70 to 75 캜 to prepare a second composition (Step S20); 95 to 99 wt% of Macadamia Nut Oil and Caprylyl Glycol And 5 to 15 wt% of the first composition, 70 to 80 wt% of the second composition, and 3 to 5 wt% of the third composition, (S40) in which 10 to 20 wt% of the composition is added to a stirrer and stirred at 5,000 to 6,000 rpm for 3 to 8 minutes, and the emulsion prepared through the primary emulsification step is dispersed in a high-pressure emulsifier at 600 to 1,000 bar A high pressure emulsification step (S50) in which the emulsion is subjected to two high pressure emulsification processes under the condition of the high pressure emulsification step (S50); and the emulsion obtained through the high pressure emulsification step is cooled to 33 ° C. at which the viscosity is completely formed, (S60); and cooling the emulsified material after the cooling step (S60) at room temperature for 20 to 30 hours St. step (S70); after a substrate has to be prepared.

However, the above-described technique includes an additional composition besides propolis, but since the first composition appears to be the minimum composition for cosmetic preparation and secondly it differs from the composition of the cosmetic composition proposed by the present applicant, The efficacy of the cosmetic composition to be proposed can not be derived.

Patent Registration No. 10-0817781 (published on March 31, 2008) Patent Registration No. 10-1410042 (issued on June 27, 2014)

An object of the present invention is to provide a cosmetic composition containing propolis as an active ingredient and a cosmetic comprising the composition.

Another object of the present invention is to provide a method for preventing skin aging or skin disease by using propolis having an efficacy such as antibacterial, anti-cancer, anti-inflammatory, antioxidant and immunity enhancement, The present invention also provides a cosmetic composition which can increase the efficacy of antioxidant, antibacterial, anti-aging, skin deflection improvement, immune stimulation, and skin disease prevention by further mixing the second agent for improving function.

To achieve the above object, the present invention provides a cosmetic composition comprising propolis as an active ingredient, comprising: a first agent having a nutritional function; And a second agent having a function of improving skin deflection, wherein the first agent and the second agent are mixed in the same ratio, wherein the first agent is selected from the group consisting of propolis extract, glycerin, , Ginseng extract, green tea extract, tocopheryl acetate, sodium hyaluronate, hydroxyethyl cellulose, dimethicone and betaglucan,

The second agent is characterized in that it comprises propolis extract, adenosine, beta glucan, Hovenia dulcis extract, green tea extract, black bean extract, acetyl hexapeptide-8, and capatripeptide-1.

3 parts by weight of glycerin, 3 parts by weight of butylene glycol, 6 parts by weight of ginseng extract, 6 parts by weight of green tea extract, and 0.03 part by weight of tocopheryl acetate, based on 100 parts by weight of the propolis extract, 0.2 parts by weight of sodium hyaluronate, 0.12 parts by weight of hydroxyethyl cellulose, 0.2 parts by weight of dimethicone and 0.3 part by weight of beta-glucan.

The second agent of the present invention may further comprise 0.05 part by weight of adenosine, 1.5 parts by weight of beta glucan, 0.7 part by weight of Hovenia dulcis, 0.7 part by weight of green tea extract, 0.5 part by weight of black bean extract, 3.0 parts by weight of acetyl hexapeptide-8 and 2.5 parts by weight of capatripeptide-1.

The propolis extract of the present invention is manufactured by mixing 2 parts by weight of propolis based on 100 parts by weight of purified water.

The cosmetic composition containing the propolis according to the present invention as an active ingredient has an advantage of preventing skin aging or skin diseases by using propolis having an antibacterial, anticancer, anti-inflammatory, anti-inflammatory, antioxidative and immunological enhancing properties as an active ingredient have.

Furthermore, by mixing the nutrient-containing first material and the skin-improving second material, it is possible to increase the efficacy of antioxidant, antibacterial, anti-aging, skin sagging, immune stimulation and skin disease prevention .

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary meanings and the inventor can properly define the concept of the term to describe its invention in the best possible way And should be construed in accordance with the principles and meanings and concepts consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification, the reference examples, and the drawings are merely the most preferred examples of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It should be understood that various equivalents and modifications may be present.

Example  1. Cosmetic composition containing propolis as an active ingredient

The cosmetic composition containing the propolis according to the present invention as an active ingredient is composed of a mixture of the first material and the second material.

Specifically, the first agent is a nutritional agent which functions as a nutrient, and contains 3 parts by weight of glycerin, 3 parts by weight of butylene glycol, 6 parts by weight of ginseng extract, 6 parts by weight of green tea extract, 0.03 part by weight of acetates, 0.2 parts by weight of sodium hyaluronate, 0.12 parts by weight of hydroxyethylcellulose, 0.2 parts by weight of dimethicone and 0.3 parts by weight of beta-glucan.

Here, the propolis extract is prepared based on the non-alcoholic water-soluble propolis production method of Registration No. 10-1486922, which the present applicant has rights to.

The propolis extract according to the present invention is prepared by mixing the propolis raw material and the ethanol capable of drinking in a ratio of 1: 4 on the weight basis, introducing it into the inside of the tank, extracting it for 7 to 14 days And a step of aging. - Extraction and aging of the extracted liquid is first filtered using a microfilter. - The extracted liquid, which has been subjected to the first-order filtration, is allowed to stand in a freezer at minus 20 degrees Celsius for 48 hours and the fat, wax and resin Removing the fat, wax, and resin components separated through the primary scavenging process from the extract; performing a secondary filtration process on the extract using a microfilter; The extraction solution subjected to the secondary filtration process has a concentration of from 55 to 60 DEG C and a vacuum of from 680 to 720 mmHg A step of performing concentration through vacuum distillation in a pressure state; mixing the weak alkaline water containing propolis extract and natural germanium produced by completing the concentration, and sodium carbonate into a tank, heating the tank at a temperature of 60 to 90 degrees Celsius Performing a dissolving step of mixing and dissolving the weak alkaline water containing the propolis extract and the natural germanium and the sodium carbonate while stirring with a stirring blade for 5 to 6 hours in a state where the dissolution process is completed, And performing a tertiary filtration process using the filtrate.

Propolis is a substance made by mixing bees with their own needles and enzymes in the same material as resin (resin) extracted from various plants for survival and propagation, and organic matter and minerals (inorganic salts) And there are about 104 kinds of other ingredients.

Among them, minerals, vitamins, amino acids, fats, organic acids and flavonoids play important roles in cell metabolism and terpenes are known to have anticancer activities.

In addition, anti-inflammatory, antioxidant, and immunity enhancement, anti-inflammatory effect is due to inhibit the enzyme producing prostaglandins in the body by propolis, antioxidant is because the flavonoid component removes active oxygen, By blocking the gene of a cancer cell before it is replicated.

In particular, the propolis has an antioxidant effect, which can lower the ending effect of the subject, so that the shelf life of the subject can be increased.

This is the same with the second measure.

Glycerin is a colorless, clear, viscous liquid with no odor, side effects, and relieves irritation of the dilute solution and softens the area. It also protects national borders, mitigates external stimuli, and prevents harmful substances from entering.

When glycerin is used in the skin, it acts to replenish the moisture of the skin and prevent drying.

Butyleneglycol is mainly used in cosmetics for the purpose of moisturizing agents, skin conditioning, viscosity reducing agents and perfumes, and also dissolves various components of the cosmetic composition according to the present invention.

Ginseng extracts help to improve skin elasticity and improve skin elasticity, help prevent dry skin, improve skin energy, improve immunity and help maintain healthy skin.

Green tea extract can be used as a soothing agent for skin, moisturizing and softening agents, and contains a flavanobonoid component, so it is effective for removing active oxygen and has excellent antioxidant activity.

Tocopheryl acetate protects the skin from harmful oxygen, helps prevent skin damage, gives conditioning to the skin and helps maintain healthy, vital and healthy skin. It is also involved in preventing moisture loss of the stratum corneum and thus helps to improve skin moisture.

Sodium hyaluronate is a component obtained by microbial strain cultivation, which removes the wrinkles on the skin and replenishes skin elasticity.

This sodium hyaluronate is a typical moisturizing ingredient that helps prevent skin from getting rough by preventing loss of moisture. It prevents external penetration from the external stimuli and germs, and can prevent trouble by omitting bacteria.

Hydroxyethyl cellulose can be used as a raw material to help stabilize emulsification so that various components contained in the cosmetic composition according to the present invention are not separated from each other. In addition, the viscosity can be increased to a jelly-like viscosity to fix the shape or improve the viscosity.

Dimethicone can be used to block moisture evaporation, act as a skin protector, and improve volatility.

Beta-glucan is a type of polysaccharide contained in the cell walls of mushrooms, yeast, and grains.

In Japan, Beta Glucan extracted from shiitake mushrooms has already been shown to be an anticancer drug "Lentinan" in the 1980s. Beta-glucan extracts from skim, mushroom, mushroom and mushroom have been launched.

This mushroom-derived beta-glucan is being used as a new moisturizing substance using natural and low molecular weight (fermentation, nano, etc.), and it is evaluated that the moisturizing function for holding the skin moisture is about 20% better than the hyaluronic acid It is a trend in the third generation cosmetics as a raw material for moisturizing.

That is, beta-glucan can perform skin moisturizing, skin nutrition and conditioning.

The second agent is an improving agent for improving skin defects that improves skin deflection, comprising 0.05 parts by weight of adenosine, 1.5 parts by weight of betaglucan, 0.7 part by weight of Hovenia dulcis extract, 0.7 part by weight of green tea extract, 0.5 part by weight of black bean extract, 3.0 parts by weight of acetyl hexapeptide-8 and 2.5 parts by weight of kappatriptide-1.

At this time, propolis extract, betaglucan and green tea extract were described in the first item, so they are omitted.

Adenosine ( A or Ado ) is a kind of ribonucleoside, which contains a purine derivative adenine at the base portion and is composed of components of RNA and many coenzymes.

Adenosine is a colorless solid that is soluble in water and does not dissolve in alcohol. It helps the synthesis of protein DNA in the dermal layer of the skin, promotes fibroblast proliferation in the dermal layer, and promotes collagen synthesis. This has the effect of improving skin elasticity and wrinkles.

The Hovenia dulcis extract is an extract of Oriental Raisin Tree , which is 10 m high and the bark is blackish gray, which is ripened vertically and peeled off, leaves are wide and egg-shaped or oval, And is known to have excellent functions such as whitening and inhibiting the generation of uric acid.

Black bean extract contains more than twice as much protein as ordinary beans, contains 18 amino acids and 8 essential amino acids , and extracts black bean (clycine soja seed extract ) rich in lecithin with various extraction methods Lecithin is a substance that regulates the moisture in the cells. It gives shine and gloss to the skin, helps absorption of vitamin E and other fat soluble vitamins, prevents skin aging, and prevents skin from being deposited. In this way, the black bean smoothes the blood circulation to the peripheral part in the body, and supplies nutrition and oxygen to the skin to make the skin clear and healthy.

Acetyl hexapaptide-8 is a non-toxic, non-toxic substance that has attracted attention as a substitute for cosmetics that eliminates concerns about side effects (such as allergic reactions, facial muscle paralysis, eyeball sagging, etc.) When applied to cosmetic raw materials, it is harmless to environment and human health.

This acetyl hexapeptide-8 is a low-molecular peptide having similar functions to the existing Botox effect. It regulates the signal transduction process of neurons connected to the Zhu Yin Muscle, thereby increasing the elasticity of the skin to enhance beauty and anti-aging function.

In addition, it is easy to penetrate the skin with low molecular weight peptides, penetrates into the skin and directly increases the skin elasticity to the muscle nerve, and can be effective in a short time. Also, the secretion of catecholamines (adrenalin and noradrenalin) It regulates the repetitive movements of the wrinkles by temporarily controlling the signaling action of the SNARE complex (which causes the muscles to repeatedly contract and relax and secrete neurotransmitters to form wrinkles) Helping skin elasticity and beauty.

Copper tripaptide-1 is a natural peptide derived from human body found in human blood and saliva.

It is present in the form bound to albumin in blood. It is present at about 300 ng / ml at 20 years of age, but decreases with aging and decreases to about 80 ng / ml at 60 years of age. This kappa tripeptide-1 is a template that is formed by degradation of proteolytic enzymes such as cytoskeletal proteins or inflammation-related proteins in the human body when a skin injury occurs. It removes damaged collagen or elastin caused by skin damage , Inhibits scarring, and is known to stimulate skin regeneration by producing stem cells. It is also known to promote hair growth and reduce hair loss.

Experimental Example  1. Sensory evaluation based on the content of the first material

1-1. Experimental course

The sensory evaluation in Experimental Example 1 was carried out immediately after cleansing once a day for 10 panelists of 30 women, 40 panelists, 10 panelists and 50 panelists, all of whom were sensitive to skin health This is done by requesting and using it for 2 weeks and then by querying.

The test group used in Experimental Example 1 was changed from the first test group to the first test group in Example 1, and specifically, the following Table 1 was used.

1-2. Experiment result

As shown in Table 1 below, the experimental group as in Example 1 and the control groups 1 to 3 in which the contents of butyleneglycol and hydroxyethyl cellulose components were changed were subjected to experimental groups according to the above experimental procedure Respectively.

Experimental Example 1 Control 1 Control group 2 Control group 3 Propolis extract 100 100 100 100 glycerin 3 3 3 3 Butylene glycol 3 ** 2* 3.5 * 4* Ginseng extract 6 6 6 6 Green tea extract 6 6 6 6 Tocopheryl acetate 0.03 0.03 0.03 0.03 Sodium hyaluronate 0.2 0.2 0.2 0.2 Hydroxyethyl cellulose 0.12 ** 0.05 * 0.12 * 0.2 * Dimethicone 0.2 0.2 0.2 0.2 Beta Glucan 0.3 0.3 0.3 0.3 (evaluation) Effectiveness satisfied satisfied satisfied dissatisfaction Feeling satisfied dissatisfaction satisfied dissatisfaction

As a result, it was found that the panels according to Experimental Example 1 were generally satisfied with the feeling of feeling of efficacy and feeling of use.

However, in the case of control group 1, the feeling of efficacy was satisfactory, but there was opinion that most of the opinion that creaminess was too low in feeling and feeling of foreign body on the surface after skin was overgrown was dissatisfied.

In the control group 2, on the average, the sensation of efficacy and feelings were satisfactory. However, 6 out of 10 people in the 30s panel (40%) had a feeling that the foreign body sensation on the surface was felt after boiling.

In the control group 3, most of them said that they were unsatisfied with the feeling of efficacy and feelings of use. Especially, there was the opinion that the feeling of feeling is difficult to use and the heavy feeling is strong after skinning. In the case of potency, it is presumed that the potency of the composition according to the content of the composition is strong, but it is presumed that the result of the potency of the potency is strong.

Experimental Example  2. Sensory evaluation according to content of second material

2-1. Experimental course

The experimental procedure was the same as in Experiment 1, except that the first material was changed to the second material, and the experiment group was changed accordingly, specifically, see Table 2 below.

2-2. Experiment result

As shown in the following [Table 2], the experiment group of Example 1 and the control groups of the control groups 1 to 3, in which the contents of some compositions were changed, were tested according to the above experimental procedure on the panels.

Example 1 Control 1 Control group 2 Control group 3 Propolis extract 100 100 100 100 Adenosine 0.05 ** 0.04 * 0.06 * 0.06 * Beta Glucan 1.5 ** 1.2 * 1.7 * 1.7 * Hodgkin's extract 0.7 ** 0.5 * 1.0 * 1.0 * Green tea extract 0.7 ** 0.5 * 1.0 * 1.0 * Black bean extract 0.5 ** 0.2 * 0.6 * 0.6 * Acetyl hexapeptide-8 3.0 ** 2.5 * 3.0 * 3.5 * Kappa-tripeptide-1 2.5 ** 1.5 * 2.5 * 2.7 * (evaluation) Satisfaction of skin improvement satisfied dissatisfaction satisfied satisfied Feeling satisfied satisfied dissatisfaction dissatisfaction

The results of the experiment according to [Table 2] show that the experimental group including Example 1 is generally satisfied with the satisfaction of skin improvement and feeling of use.

However, in the case of control group 1, the feeling of use was satisfactory, but there was opinion that the degree of skin improvement was not particularly noticeable in the majority of the 50-year-old female panel.

This was the same opinion from three of the women in their 40s who were 30%.

In the control group 2, most of the opinions were satisfied that the satisfaction of the skin improvement was satisfied. However, more than half said that they felt discomfort after wearing the skin.

In the control group 3, there was the opinion that the satisfaction of the skin improvement was satisfied, but most of the opinions were inconvenient to use because the sensation felt on the skin was very heavy.

Experimental Example  3. Sensory evaluation according to the first, second and first and second materials

3-1. Experimental course

The experiment according to Experimental Example 3 was performed on the same panels as Experimental Example 1, and the same procedure was performed.

However, the use of the first material, the second material, and the first material + the second material, respectively, may be used only when the first material is used or when the second material is used, and the first material and the second material are used together , The degree of change of the skin was natural because it was good when used together. Therefore, evaluation of the feeling of use was requested not to evaluate the degree of change of the skin.

In the case of the first material and the second material, the contents of Example 1 are applied according to the results of Experimental Example 1 and Experimental Example 2, and the materials of the first material and the second material are mixed in a ratio of 1: 1 .

In addition, the use feeling was analyzed by using the 5 point scale from 1 to 5 to reflect the opinion of the panel in order to proceed in more detail, and classified into degree of blushing, lightness after wearing, moistness and feeling of comprehensive feeling. The closer to 5, the higher the satisfaction.

3-2. Experiment result

See Table 3 for experimental results.

First material Second material First material + second material About amount 4.55 4.57 4.65 Lightness after wearing 4.24 4.12 4.13 Moistness 4.47 4.52 4.63 Comprehensive feeling 4.05 4.01 4.14

According to Table 3, both the first, second, and first and second items showed a significant level of information on the balance, information, lightness, and moisture, And 4.14 for the first and second sanctions.

These results show that when cosmetics are launched through the cosmetic composition according to the present invention, cosmetics containing a first agent and a second agent capable of simultaneously performing the nutritional function of the first agent and the skin deflection improvement function of the second agent , It is expected that consumer responses will be higher than launching cosmetics consisting of only sanitary materials.

However, when the first material and the second material were mixed, Experimental Example 3 was performed on the basis of the same ratio of 1: 1. Therefore, in the following Experimental Example 4, sensory evaluation was performed by mixing ratio.

Experimental Example  4. Sensory evaluation by mixing ratios of the first and second materials

4-1. Experimental course

In Experimental Example 4, based on the results of Experimental Examples 1 to 3, the experimental group was composed of a cosmetic composition comprising a first agent and a second agent. Specifically, the experimental group was as follows.

Experimental group 1: mixing the first material and the second material in a 1: 2 ratio

Experimental group 2: mixing the first material and the second material at a ratio of 1: 1.5

Experimental group 3: mixing the first material and the second material at a ratio of 1: 1

Experimental group 4: mixing the first material and the second material at a ratio of 1.5: 1

Experimental group 5: mixing the first material and the second material at a ratio of 2: 1

The above experimental groups 1 to 5 were used in the same panel as Experimental Example 1 to evaluate sagging improvement, moisture retention, and feeling of use, and a 5-point scale was calculated based on the panel evaluation. This means that the closer to 5 points the higher the function (satisfaction).

4-2. Experiment result

The results of the experiment according to Experimental Example 4 are shown in Table 4 below.

Experiment 1 Experiment 2 Experiment group 3 Experiment group 4 Experiment group 5 Improve deflection 4.78 4.62 4.61 3.89 3.92 Moisture retention 4.30 4.31 4.33 4.31 4.32 Feeling 3.97 4.02 4.21 3.95 3.92

[Table 4] shows that the improvement of sag in Experiment Groups 1 and 3 was significant (although it was obvious that the effect was higher with higher proportion of the second material) As the proportion of remedies was decreased, it was lower than that of experimental groups 1 to 3.

And the degree of moisture retention were all significant. For the sensation of feeling, the feeling of use when the first and second materials were mixed at the same ratio was the highest at 4.21, and as the second material was mixed more than the first material The feeling of use was evaluated low.

In addition, when the first material is mixed more than the second material, the degree of evaluation is lower than that of the second material mixed more than the first material. Especially, the higher the mixing ratio of the first material, Satisfaction was low.

Based on this, it can be predicted that the first material and the second material are more mixed with each other than the other one, so that the first material and the second material can be used at the same ratio (1: 1 ). ≪ / RTI >

Based on the above-described contents, the cosmetic composition containing the propolis according to the present invention as an active ingredient can be used to produce cosmetics. The cosmetics include nutritive creams, skins, lotions, foam cleansing, essences, And cosmetics that are associated with the skin and help to improve the skin.

Claims (5)

A first material; And a second agent, wherein the first agent and the second agent are mixed in the same ratio,
The first material,
100 parts by weight of propolis extract, 3 parts by weight of glycerin, 3 parts by weight of butylene glycol, 6 parts by weight of ginseng extract, 6 parts by weight of green tea extract, 0.03 part by weight of tocopheryl acetate, 0.2 parts by weight of sodium hyaluronate, 0.12 parts by weight of ethylcellulose, 0.2 parts by weight of dimethicone and 0.3 parts by weight of beta-glucan,
The second material,
0.7 part by weight of green tea extract, 0.5 part by weight of black bean extract, 3.0 parts by weight of acetylhexapeptide-8, and 0.1 part by weight of capatripeptide were mixed with 100 parts by weight of propolis extract, 0.05 part by weight of adenosine, 1.5 parts by weight of betaglucan, -1, 2.5 parts by weight,
The propolis extract,
A cosmetic composition comprising propolis as an active ingredient, characterized in that it is produced by a process for producing non-alcoholic water-soluble propolis.
delete delete delete A cosmetic comprising the cosmetic composition according to claim 1.