KR101786893B1 - Feed compositions for production of milk comprising improved ratio of fatty acids and omega-3,6 - Google Patents
Feed compositions for production of milk comprising improved ratio of fatty acids and omega-3,6 Download PDFInfo
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- KR101786893B1 KR101786893B1 KR1020170101460A KR20170101460A KR101786893B1 KR 101786893 B1 KR101786893 B1 KR 101786893B1 KR 1020170101460 A KR1020170101460 A KR 1020170101460A KR 20170101460 A KR20170101460 A KR 20170101460A KR 101786893 B1 KR101786893 B1 KR 101786893B1
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- milk
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- 235000014113 dietary fatty acids Nutrition 0.000 title claims abstract description 36
- 229930195729 fatty acid Natural products 0.000 title claims abstract description 36
- 239000000194 fatty acid Substances 0.000 title claims abstract description 36
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- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 claims abstract description 10
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- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/18—Lipids
- A23V2250/186—Fatty acids
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
본 발명은 지방산 함량 및 오메가-3, 6의 비율이 개선된 우유를 생산하기 위한 사료조성물에 관한 것으로, 구체적으로 지방산 공급원으로서 아마씨, 캐놀라씨 및 어유를 2 내지 4 중량%, 부형제로서 수수, 기장 및 완두콩을 2 내지 4 중량%를 포함하는 것을 특징으로 하는, 기능성 지방산 함량 및 오메가-3와 6의 비율이 개선된 우유를 생산하기 위한 유우용 사료 조성물에 관한 것이다.The present invention relates to a feed composition for producing milk having improved fatty acid content and omega-3, 6 ratio, and more particularly to a feed composition comprising 2 to 4% by weight of flaxseed, canola and fish oil as fatty acid sources, And 2 to 4% by weight of peas. The present invention relates to a dairy composition for producing milk with improved functional fatty acid content and a ratio of omega-3 to omega-6.
Description
본 발명은 지방산 함량 및 오메가-3, 6의 비율이 개선된 우유를 생산하기 위한 사료조성물에 관한 것으로, 구체적으로 지방산 공급원으로서 아마씨, 캐놀라씨 및 어유를 2 내지 4 중량%, 부형제로서 수수, 기장 및 완두콩을 2 내지 4 중량%를 포함하는 것을 특징으로 하는, 기능성 지방산 함량 및 오메가-3와 6의 비율이 개선된 우유를 생산하기 위한 유우용 사료 조성물에 관한 것이다.The present invention relates to a feed composition for producing milk having improved fatty acid content and omega-3, 6 ratio, and more particularly to a feed composition comprising 2 to 4% by weight of flaxseed, canola and fish oil as fatty acid sources, And 2 to 4% by weight of peas. The present invention relates to a dairy composition for producing milk having improved functional fatty acid content and a ratio of omega-3 to omega-6.
우유 200 mL 1컵에는 8g의 지방과 5g의 포화지방이 함유되어 있는데, 이것은 1일 영양소 기준치의 16%와 33%에 해당된다. 포화지방의 과다 섭취는 각종 성인병의 원인이 될 수 있어 우유 음용을 싫어하는 사람들의 핑계가 되고 있다. 오메가-6 지방산의 비율이 높은 지방은 심장질환, 암, 염증, 자가면역 질환 등을 증가시키고, 반대로 오메가-3 지방산의 함량이 높으면, 억제효과를 나타낸다(Simopoulos, 2002).One 200 mL of milk contains 8 g of fat and 5 g of saturated fat, which corresponds to 16% and 33% of the daily nutrient standard. Excessive intake of saturated fats can be a cause of various adult diseases, making it an excuse for people who do not like to drink milk. High fat omega-6 fatty acids increase heart disease, cancer, inflammation, autoimmune disease, and, on the contrary, higher levels of omega-3 fatty acids (Simopoulos, 2002).
우유의 지방산 조성은 사료에 따라 변화될 수 있으며, DHA(docosa hexaenoic acid), CLA(conjugated linoleic acid), 오메가-3 조성을 달리한 우유가 판매되고 있다. 건강기능식품공전에 의하면, EPA(Eicosapentaenoic acid)와 DHA의 기능성은 혈중 중성지질 개선, 혈행 개선에 도움을 주는 것으로 되어 있으며, CLA는 과체중인 성인의 체지방 감소에 도움을 줄 수 있는 것으로 되어 있다. 이외에도 EPA와 DHA는 인지 능력 개선, 항암 세포 억제, 항염증 반응 등의 효과가 있는 것으로 보고되어 있으며, EPA와 DHA의 섭취 부족은 태아의 발육 저해, 일반적 심혈관계 질환, 알츠하이머 질병의 발병 위험과 연관이 있는 것으로 알려져 있다. CLA는 항암, 콜레스테롤 저하 및 당뇨병에 효능이 있는 것으로 보고되어 있다. VA(trans-vaccenic acid)는 인체에서 흡수된 양의 19% 정도가 CLA로 전환된다. Fatty acid composition of milk can be changed according to the diet, and milk with docosahexaenoic acid (DHA), conjugated linoleic acid (CLA) and omega-3 composition is sold. According to the Health Functional Food Cycle, the functionality of EPA (Eicosapentaenoic acid) and DHA has been shown to improve blood neutrality and blood circulation, and CLA has been shown to help reduce body fat in overweight adults. In addition, EPA and DHA have been reported to improve cognitive function, to inhibit cancer cells, and to have an anti-inflammatory effect. The lack of intake of EPA and DHA is associated with the development of fetal development, general cardiovascular disease and the risk of developing Alzheimer's disease . CLA has been reported to be effective in anti-cancer, cholesterol lowering and diabetes. VA (trans-vaccenic acid) is converted to CLA by 19% of the amount absorbed from the human body.
기존 우유의 지방의 조성을 EPA, DHA, CLA, VA의 함량을 높이고, 건강한 지방산 비율인 오메가-3와 오메가-6 비율을 1:4 이하의 비율로 바꾼다면 보다 건강한 우유가 가능할 것이다(Simopoulos, 2002). 그러나, 국내에 사료 유래 우유 지방산 조성을 변화시킨 제품으로 판매되고 있는 제품으로는 DHA를 일반 우유 대비 높인 우유, CLA 함량을 일반 우유 대비 높인 우유, 오메가-3와 오메가-6 비율을 1:4의 조성으로 변화시킨 우유는 판매되고 있으나, 각각을 하나의 제품으로 구현한 제품은 아직 판매되지 않고 있다.Healthy milk will be possible if the content of EPA, DHA, CLA, and VA is increased and the proportion of healthy fatty acids, omega-3 and omega-6, is changed to 1: 4 or less (Simopoulos, 2002 ). However, the products that have been modified to produce milk-derived milk fatty acid composition in Korea include DHA as milk, CLA as milk, omega-3 and omega-6 ratios of 1: 4 Has been sold, but the product that implements each product as a single product has not been sold yet.
한편, 우유의 사료 유래 기능성 지방산 함량을 높일 수 있는 방법에 대한 국내 특허로는, 특허공보 특1995-0006782(1995.06.22) "n-3 지방산이 축적된 우유 및 유우용 사료조성물", 특허공보 특1996-0014849(1996.10.21) "고도다중 불포화지방산 함량이 높은 우유를 생산키 위한 사료첨가제의 제조방법", 등록특허공보 10-0431906(2004.05.17) "우유 CLA 함량을 높이는 사료 및 그 제조방법", 등록특허공보 10-1187697(2012.10.05) "omega-3 지방산을 함유하는 우유를 생산하기 위한 수용성 아마씨유 함유 사료첨가제 조성물" 등이 있다. On the other hand, as a domestic patent for a method for increasing the functional fatty acid content derived from milk from milk, Patent Document No. 1995-0006782 (1995.06.22) entitled "n-3 fatty acid-accumulated milk and dairy cat food composition" 1996-0014849 (October 21, 1996) "A method for producing a feed additive for producing milk having a high polyunsaturated fatty acid content", Registered Patent Publication 10-0431906 (2004.05.17) "And a water-soluble flaxseed oil-containing feed additive composition for producing milk containing omega-3 fatty acid, etc., and the like are disclosed in Patent Document 10-1187697 (Oct.
특허공보 특1995-0006782(1995.06.22)는 DPA, EPA, DHA를 포함하는 오메가-3 지방산 함량을 높이기 위해 어분, 유화보조제를 함유하고 있다. 특허공보 특1996-0014849(1996.10.21 공고)는 어유를 카올린과 상온에서 혼합하여 압출 성형시켜 과립화하고, 과립 어유에 제인, 셀락, 팔미틴산 및 프로필렌글리콜로 이루어지는 복합 피복제를 피복하여 건조시킨 사료첨가제의 제조방법에 대한 특허이다. 등록특허공보 10-0431906(2004.05.17)은 사료 조성물에 열처리 전지대두를 단독으로 또는 열처리 전지대두 및 아마인유를 혼합첨가한 전체 사료를 고온에서 단시간 열처리한 사료를 젖소에 급여하여 CLA 함량을 증가시킨 특허로 실시 예에서 CLA 함량은 11.85±0.52mg/g까지 높아지는 것으로 보고하고 있다. 등록특허공보 10-1187697(2012.10.05)은 아마씨유와 녹차 카테킨추출물을 혼합한 첨가제를 젖소에 급여하여 omega-3 함량을 증가시키는 내용이다. Patent Publication No. 1995-0006782 (June 22, 1995) contains fish meal and emulsifying adjuvant to increase omega-3 fatty acid content including DPA, EPA and DHA. Patent Publication No. 1996-0014849 (published on Oct. 21, 1996) discloses a method of mixing fish oil with kaolin at room temperature, granulating it by extrusion molding, coating a composite coating comprising jane, cellak, palmitic acid and propylene glycol in granular fish oil, It is a patent for the manufacturing method of additives. Patent Document 10-0431906 (Apr. 17, 2004) discloses a method of increasing the amount of CLA by feeding a heat-treated cellulosic soybean to a feed composition, or a short-term heat-treated feed of whole feeds containing heat-treated cellulosic soybean and linseed oil, , The CLA content in the examples is increased to 11.85 ± 0.52 mg / g. Patent Application No. 10-1187697 (Oct. 20, 2012) discloses that omega-3 content is increased by feeding an additive mixture of flaxseed oil and green tea catechin extract to a cow.
이처럼 국내 특허도 하나의 사료 조성물로 CLA, VA, DHA, EPA 함량을 증가시키고, 오메가-3와 6의 비율은 1:4로 구현하지는 못하고 있다. Thus, the domestic patent also increases the contents of CLA, VA, DHA and EPA with one feed composition, and the ratio of omega-3 to 6 is not 1: 4.
이에 본 발명자들은 EPA, DHA 함량과 CLA 함량을 증가시키면서 오메가-3와 6의 비율을 1:4로 함께 구현할 수 있는 제품을 개발하고자 연구 하였다.Therefore, the present inventors have studied to develop a product which can increase the content of EPA, DHA and CLA, and can achieve the ratio of 1: 4 omega-3 and 6 at the same time.
따라서 본 발명의 목적은, 젖소에게 급여하여 인체에 유용한 EPA, DHA, CLA, VA 지방산 함량을 증가시키는 동시에, 오메가-3와 오메가-6의 비율을 1:4로 변화시킨 건강한 지방산을 갖는 우유를 생산하기 위한 사료 조성물을 개발하는데 있다. Accordingly, an object of the present invention is to provide a milk having a healthy fatty acid in which the ratio of omega-3 to omega-6 is changed to 1: 4 while the content of EPA, DHA, CLA, And to develop a feed composition for production.
본 발명에 따른 상기 목적을 달성하기 위하여, 본 발명은 조사료(roughage), 부산물사료(by-product feed), 농후사료(concentrated feed stuff), 및 첨가제로 구성되는 유우용 사료 조성물에 있어서, 전체 사료 조성물에 대하여 지방산 공급원으로서 아마씨, 캐놀라씨 및 어유를 2 내지 4 중량%, 부형제로서 수수, 기장 및 완두콩을 2 내지 4 중량%를 포함하는 것을 특징으로 하며, 기능성 지방산으로서 VA(trans-vaccenic acid), CLA(conjugated linoleic acid), EPA(Eicosapentaenoic acid), DHA(docosa hexaenoic acid)를 모두 포함하고 오메가-3와 6의 비율이 1:1 내지 1:4의 지방산 조성을 갖는 우유를 생산하기 위한 유우용 사료 조성물을 제공한다.In order to achieve the above object, the present invention provides a dairy composition comprising a roughage, a by-product feed, a concentrated feed stuff, and an additive, wherein the total feed composition 2 to 4% by weight of flaxseed, canola and fish oil as fatty acid sources and 2 to 4% by weight of sorghum, millet and peas as excipient, wherein the functional fatty acid is VA (trans-vaccenic acid) The present invention relates to a dairy composition for producing milk having a fatty acid composition of 1: 1 to 1: 4 in the ratio of omega-3 to 6: CLA (conjugated linoleic acid), EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) .
본 발명에서, 상기 "조사료(roughage)"는 목초, 건초, 사일리지, 옥수수, 파, 씨 있는 과일의 껍데기 등 섬유질로, 에너지함량(가소화양분함량)이 적은 사료를 의미한다. 가축, 특히 초식동물의 사료이다. 반추위를 갖는 가축에서는 생리적으로 어느 정도의 양은 유지되어야 한다. 또 근채류는 그 자체가 수분함량이 높기 때문에 조사료(租飼料)로 취급되고 있지만, 건조하면 농후사료로 취급된다 (영양학사전, 1998. 3. 15., 아카데미서적). In the present invention, the term " roughage " means a feed having a low energy content (plasticized nutrient content), such as grasses, hay, silage, corn, It is a feed for livestock, especially herbivores. In livestock with rumen, physiologically some amount should be maintained. In addition, root vegetables are treated as roughage because they have high water content, but they are treated as concentrated feeds when dried (Nutrition Dictionary, March 15, 1998, Academy Book).
상기 "부산물사료(by-product feed)"는 농산물 또는 식품의 제조 과정에서 나오는 각종 강피류(糠皮類), 박류(粕類) 등 사료로 이용되는 2차 산물을 의미한다 (농업용어사전: 농촌진흥청). The "by-product feed" mentioned above means a secondary product used as feed for various agricultural products or various kinds of bark (peeled bark) Rural Development Administration).
상기 "농후사료(concentrated feed stuff)"는 곡류ㆍ겨류ㆍ깻묵류가 이에 속한다. 이 사료는 가소화 성분은 많으나 그 성분이 한쪽으로 치우친 것이 많으며, 성질도 각각 다른 것이 많으므로, 실제 사료를 배합할 때에는 여러 종류의 농후사료를 배합하여야 한다. 농후사료는 주로 비육(肥肉)하는 가축과 젖ㆍ알 등을 생산하는 가축에게 많이 쓰인다 (두산백과).The term " concentrated feed stuff "as used herein includes cereals, beans, and calamari. This feed contains many plasticizers, but many of the ingredients are biased to one side, and many of them have different properties. Therefore, when mixing actual feeds, various kinds of concentrated feeds should be added. Concentrated feed is mainly used for livestock and cattle producing milk and eggs (Doosan Encyclopedia).
이러한 조사료, 부산물사료, 농후사료 및 사료 첨가제가 포함되어 일반적인 배합 사료가 제조된다.Typical formulated feeds are made up including such forage, by-product feed, concentrated feed and feed additives.
본 발명에서는, 일반적인 배합 사료에 특정 종자와 어유, 그리고 부형제를 특정 비율로 함유시킴으로써, 여러 기능성 지방산을 함유케 하고 오메가 3, 6의 비율을 개선시킬 수 있는 새로운 조성비를 갖는 기능성 사료 조성물을 개발하였다.In the present invention, a functional feed composition having a novel composition ratio capable of containing various functional fatty acids and improving the ratio of omega 3, 6 was developed by containing a specific seed, fish oil, and excipient at a specific ratio in a general compound feed .
일반적으로 착유 젖소의 사료 내 지방산 함량은 4~5% 정도이며, 사료를 통해 반추위로 유입된 지방은 트리글리세라이드, 인지질, 당지질의 에스테르 결합이 가수분해된다. 반추위의 가수분해는 반추위 미생물의 지방 분해 효소에 의해 일어나며, 다가불포화지방산의 생체내 경화작용이 그 다음으로 진행된다. LA(linoleic acid)와 LNA(α-linolenic acid)는 cis-12의 이중 결합이 trans-12로 배열되는 이성질체화와 cis-9의 이중 결합의 환원반응에 rumenic acid가 중간 대사물질로 생성되며, rumenic acid는 VA로 전환되어 최종 물질로 스테아르산(stearic acid)이 생성된다. 내인성 CLA 합성도 진행되는데 Δ9-desaturase의 작용에 의해 일어난다.Generally, the amount of fatty acid in the feed of milk cows is about 4 ~ 5%, and the fat introduced into the rumen through the feed hydrolyzes the ester bond of triglyceride, phospholipid and glycolipid. Hydrolysis of the rumen is caused by lipolytic enzymes of rumen microbes, followed by in vivo curing of polyunsaturated fatty acids. Linolenic acid (LA) and α-linolenic acid (LNA) are produced by rumenic acid as an intermediate metabolite in the isomerization of double bond of cis-12 to trans-12 and the reduction of double bond of cis-9, rumenic acid is converted to VA and stearic acid is produced as a final product. Endogenous CLA synthesis also proceeds by the action of Δ9-desaturase.
본 발명의 조성물은, 지방산 공급원으로서 특정 종자 및 어유를 이용하고 부형제로서 특정 곡류를 포함하며 그 함량을 조절함으로써 기능성 사료로서 이용될 수 있다. 이러한 사료 조성물을 급여하여 사육된 젖소로부터 얻은 우유는 기능성 지방산의 함유량이 높아질 뿐만 아니라 오메가-3, 6의 균형 있는 비율(1:4 이하)로 조절될 수 있어 건강한 우유를 원하는 소비자의 욕구를 만족시킬 수 있다.The composition of the present invention can be used as a functional food by using specific seeds and fish oil as a fatty acid source and containing specific grains as an excipient and controlling its content. Milk derived from cows raised by feeding these feed compositions not only has higher functional fatty acid content but also can be adjusted to a balanced ratio of omega-3, 6 (1: 4 or less), satisfying the desire of consumers who desire healthy milk .
상기와 같은 기능성이 강화된 우유를 생산하기 위한 사료 조성물은 지방산 공급원으로서 아마씨, 캐놀라씨 및 어유를 2 내지 4 중량%, 부형제로서 수수, 기장 및 완두콩을 2 내지 4 중량%를 포함하는 것을 특징으로 한다. 특히 본 발명의 사료 조성물을 젖소에 급여하여 생산된 우유는 기능성 지방산으로서 VA, CLA, EPA, DHA를 모두 포함하고 오메가-3와 6의 비율이 1:1 내지 1:4의 지방산 조성을 갖는 장점이 있다. The feed composition for producing milk having enhanced functionality as described above is characterized by containing 2 to 4% by weight of flaxseed, canola and fish oil as a fatty acid supply source, 2 to 4% by weight of millet, millet and pea as an excipient do. In particular, the milk produced by feeding the feed composition of the present invention to cows has the advantage that it contains all of VA, CLA, EPA and DHA as functional fatty acids and has a fatty acid composition with a ratio of omega-3 to 6 of 1: 1 to 1: 4 have.
본 발명의 바람직한 구현예에서, 상기 사료 조성물에 포함되는 아마씨, 캐놀라씨 및 어유는 각각 1.80, 0.90 및 0.15 중량%일 수 있다.In a preferred embodiment of the present invention, the flaxseed, canola seed and fish oil contained in the feed composition may be 1.80, 0.90 and 0.15 wt%, respectively.
또한 상기 수수, 기장 및 완두콩은 각각 1.5, 1.35 및 0.3 중량%일 수 있다. The sorghum, millet and pea may be 1.5, 1.35 and 0.3 wt%, respectively.
본 발명의 다른 바람직한 구현예에서, 상기 사료 조성물의 전체적인 함량비는 연맥 6-8 중량%, 알팔파 6-8 중량%, 티모시 6-8 중량%, 톨페스큐 4-6 중량%, 비트펄프 5-7 중량%, 면실 3-5 중량%, 수수 1-2 중량%, 기장 1-2 중량%, 아마씨 1-3 중량%, 완두콩 0.1-1.0 중량%, 캐놀라씨 0.1-2.0 중량%, 어유 0.05-0.2 중량%, TMR 콘믹스 15-25 중량%, 브로피드 5-15 중량%, 물 20-30 중량%를 포함할 수 있다.In another preferred embodiment of the invention, the overall feed ratio of the feed composition is 6-8% by weight oat, 6-8% by weight alfalfa, 6-8% by weight timothy, 4-6% by weight tall fescue, 5-7% 1 to 2% by weight of filaments, 1 to 3% by weight of flaxseed, 0.1 to 1.0% by weight of peas, 0.1 to 2.0% by weight of canola seeds, 0.05 to 0.2% of fish oil 15-25% by weight of TMR cone mix, 5-15% by weight of bromide, and 20-30% by weight of water.
본 발명의 실시예에 따르면, 상기의 특징적인 함량비를 갖는 사료 조성물을 급여하고 그에 따른 기능성 지방산 함량비를 확인한 결과, 충분한 양의 기능성 사료를 6주간 급여한 젖소로부터 얻은 원유에는 VA, CLA, EPA 및 DHA가 모두 높게 함유되어 있었으며 특히 오메가-3, 6의 비율이 1:3.9로서 가장 좋은 지방산 분석 결과를 나타내었다 (실시예 2, 표 5 참조). 이는 종래 CLA, DHA 등 특정 지방산의 함유량만 강화시킨 우유와 달리 기능성 지방산을 모두 함유하고 있다는 점에서 현저한 효과 차이로 볼 수 있으며 이와 동시에 건강한 오메가 3, 6의 비율을 유지시킬 수 있는 장점도 가지고 있으므로, 우수한 기능성, 기호성, 상품성을 갖춘 우유의 생산에 이용될 수 있음을 의미하는 것이다.According to the embodiment of the present invention, as a result of feeding the feed composition having the characteristic content ratio and checking the functional fatty acid content ratio, the crude oil obtained from the cow fed with a sufficient amount of the functional food for 6 weeks contained VA, CLA, EPA and DHA were all highly contained. Especially, the ratio of omega-3 and 6 was 1: 3.9, and the best fatty acid analysis results were obtained (see Example 2 and Table 5). Unlike milk, which contains only specific fatty acid contents such as CLA and DHA, it has a remarkable effect in that it contains all the functional fatty acids. At the same time, it has the advantage of maintaining the ratio of healthy omega 3 and 6 , And can be used for the production of milk having excellent functionality, palatability and merchantability.
상기에서 살펴본 바와 같이, 본 발명의 사료 조성물을 급여한 젖소에서 착유한 원유는 EPA와 DHA, CLA와 VA의 함량을 높이고, 건강한 지방산 조성인 오메가-3와 오메가-6의 1:4의 비율을 갖는다. 기존의 불포화 지방산 및 CLA 전구물질을 젖소에 다량 급여 시 젖소의 반추위내 미생물이 불포화 지방산을 포화 지방산으로 변환시켜 우유내 포화 지방산이 증가하는 경향이 발생하는데, 이 점을 극복하고 기호성 개선으로 젖소의 사료 섭취량이 증가하여 EPA, DHA, CLA, VA와 오메가-3 조성이 개선된 우유를 생산하기 위해 이용될 수 있다.As described above, crude oil milked in the cow fed with the feed composition of the present invention increased the contents of EPA, DHA, CLA and VA and increased the ratio of 1: 4 omega-3 and omega-6, which are healthy fatty acid compositions . In the case of feeding large amounts of unsaturated fatty acids and CLA precursors to cows, the microorganisms in the cows' rumen tend to convert unsaturated fatty acids into saturated fatty acids, which leads to an increase in saturated fatty acids in the milk. Increased feed intake led to improved EPA, DHA, CLA, VA and omega-3 composition It can be used to produce milk.
이하, 본 발명을 실시예에 의해 상세히 설명하기로 한다. 그러나 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.
실시예 1 : 기능성 사료의 제조Example 1: Preparation of functional feeds
본 발명자들은 지방산 함량 및 오메가-3와 6 비율을 개선한 우유를 생산할 수 있는 배합사료를 개발하고자 연구한 끝에, 기름 함량이 높은 종자와 어유, 부형제로 포함하는 기능성 사료 조성물을 고안하였다. 본 발명에서는 기름이 풍부한 종자로 아마씨, 캐놀라씨를 사용하였고, 부형제로 수수, 기장, 완두콩을 사용하였다. 하기 표 1은 일반적인 젖소 사료에 아마씨, 캐놀라씨, 어유, 부형제를 혼합한 배합비의 예이다.The inventors of the present invention have studied a feed composition capable of producing milk with improved fatty acid content and omega-3 and 6 ratio, and then devised a functional feed composition containing seeds with high oil content, fish oil and excipients. In the present invention, flaxseed and canola seeds were used as oil-rich seeds, and sorghum, millet, and pea were used as excipients. Table 1 below shows an example of the blending ratio of common fodder fodder mixed with flaxseed, canola seed, fish oil, and excipient.
하기 표 1에 따른 조사료 원료는 수입 업체 및 서울우유 지정 업체로부터 구입하여 사용하였다. 배합사료(콘믹스, 믹스)는 서울우유에서 지정한 배합사료 공장으로부터 구입하여 사용하였다. 시험에 사용한 어유로는 정어리유, 연어유, 대구간유가 혼합된 캐나다산을 사용하였다. 하기 표 1의 배합비율에 따라 아래 표 2의 제조공정도에 따라 원료를 측량한 후, 30분간 배합기에서 혼합하여 시험용 사료를 조제하였다. The raw materials according to Table 1 were purchased from importers and Seoul Milk designated companies. Mixed feeds (cone mixes and mixes) were purchased from a compound feed factory designated by Seoul Milk and used. The fish oil used in the test was Canadian acid mixed with sardine oil, salmon oil and cod liver oil. The raw materials were weighed according to the mixing ratio shown in the following Table 1 according to the manufacturing process chart shown in Table 2, and then mixed for 30 minutes in a blender to prepare a test feed.
상기 표 2에서 보충 사료는 마이크로 도징빈에 공급하였고, 조사료는 두과, 화본과, 양초 부분의 입자가 긴 풀사료를 의미한다. 상기 농후 사료는 배합사료 공장에서 취급하는 다량 사용 원료로서 가공하여 펠렛이나 크럼블 사료도 포함된다. 상기 발효 사료는 수분이 많이 포함되어 있는 원료로 구성되어 있다. 상기 액상 원료는 물이나 당밀과 기타 주정 원료를 품질 규격에 맞추어 관리하였다.In Table 2, the supplementary feeds were supplied to the microorganisms, and the forage means long-grain feedstuffs in which the particles of the two parts, the camphor and the candle parts are long. These concentrated feeds are processed as bulk feedstocks for use in compound feed mills and include pellets or crumbled feed. The fermented feed is composed of a raw material containing a large amount of water. The liquid raw materials were controlled according to quality standards such as water, molasses and other raw materials.
상기 배합비로 만든 배합사료(TMR)의 일반 성분은 하기 표 3과 같다.Table 3 shows the general components of the compound feed (TMR) prepared at the above mixing ratio.
NDF, neutral detergent fiber : 중성 세제 불용성 식이섬유NDF, neutral detergent fiber: neutral detergent insoluble fiber
ADF, acid detergent fiber : 산성 세제 불용성 식이섬유ADF, acid detergent fiber: acid detergent insoluble fiber
실시예 2 : 사료 급여량에 따른 유질 및 유량 변화Example 2: Oil quality and flow rate change according to feeding amount of feed
상기 실시예 1에서 제조된 TMR 기능성 사료를 5일 동안 유우(젓소)에 급여할 수 있는 양으로 만들어 두 군데의 시험 목장(A, B)에 공급하였다. 시험 사료를 급여한 목장의 유질 및 유량 변화는 7주간 확인하였으며, 그 결과는 시험 목장(A)의 결과는 하기 표 4에, 시험 목장(B)의 결과는 하기 표 5에 각각 나타내었다. The TMR functional feed prepared in Example 1 was fed to the two test ranches (A, B) in an amount that could be fed to the cows for 5 days. Changes in oil quality and flow rate of the ranch fed with the test diets were confirmed for 7 weeks. The results are shown in Table 4 and Table 5, respectively.
상기 표 4과 5에서 확인할 수 있듯이, 본 발명의 배합 사료를 급여한 경우 유지방 함량은 다소 감소하지만, 단백질, 체세포수, 세균수, 젖소 두당 유량 변화에는 영향이 없는 것으로 나타났다.As shown in Tables 4 and 5, when the compounded diets of the present invention were fed, the milk content was slightly decreased, but the protein, somatic cell count, bacterial count, and cow milk flow rate were not affected.
실시예 3 : 사료 급여량에 따른 기능성 지방산 조성 변화Example 3: Functional fatty acid composition change according to feeding amount of feed
상기 실시예 2와 동일한 방식으로 기능성 사료를 급여한 후, 시험 목장 A와 B에서 각 급여 기간의 지방산 조성의 변화를 분석하고 그 결과를 각각 하기 표 6와 표 7에 나타내었다.After feeding the functional feeds in the same manner as in Example 2, changes in the fatty acid composition of each feeding period were analyzed in the test ranches A and B, and the results are shown in Tables 6 and 7, respectively.
VA, trans vaccenic acid; LA, linoleic acid; LNA, α-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acidVA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid
VA, trans vaccenic acid; LA, linoleic acid; LNA, α-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acidVA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid
상기 표 6와 7에서 확인할 수 있듯이, 시험 목장(A)에서는 기능성 사료를 1.8kg 급여한 6주차에서 원유 중의 VA 함량 15.2 mg/100mL, CLA 함량 20.6 mg/100mL, EPA 함량 1.49 mg/100mL, DHA 함량 1.42 mg/100mL, 그리고 오메가-3:6 비율 1:3.9의 가장 좋은 지방산 분석 결과를 보였다. As shown in Tables 6 and 7, in the 6th week after feeding 1.8 kg of the functional food, VA content 15.2 mg / 100 mL, CLA content 20.6 mg / 100 mL, EPA content 1.49 mg / 100 mL, DHA , 1.42 mg / 100 mL, and an omega-3: 6 ratio of 1: 3.9.
시험 목장(B)에서도 기능성 사료를 1.8kg 급여한 6주차에서 원유 중의 VA 함량 15.1 mg/100mL, CLA 함량 21.3 mg/100mL, EPA 함량 1.12 mg/100mL, DHA 함량 1.3 mg/100mL, 그리고 오메가-3:6 비율 1:5.2의 지방산 분석 결과를 보였다. 기능성 사료 급여량을 더 증가시켰을 때, 오메가-3와 6의 비율은 긍정적인 결과를 보였으나, 기능성 지방산 함량은 감소하였다.In the 6th week after the feeding of 1.8kg of the functional diet, the experimental ranch (B) also had a VA content of 15.1 mg / 100 mL, CLA content of 21.3 mg / 100 mL, EPA content of 1.12 mg / 100 mL, DHA content of 1.3 mg / 100 mL, : 6 ratio of 1: 5.2. When the feed of functional diets was further increased, the ratio of omega-3 to omega-6 was positive but the functional fatty acid content was decreased.
비교예 : 시판 우유의 지방산 분석Comparative Example: Fatty Acid Analysis of Commercial Milk
하기 표 8은 현재 국내에서 시판되고 있는 다양한 우유에 대한 지방산 분석 결과이다. 하기 표 8에서 A사는 SPC 밀크플러스, B사는 매일유업 상하 유기농우유, C사는 남양유업 아인슈타인 및 D사는 서울우유의 일반 우유를 나타낸다.Table 8 below shows the fatty acid analysis results for various milk products currently available in Korea. In Table 8 below, A represents SPC Milk Plus, B represents daily milk-lower organic milk, C: Namyang Dairy Einstein, and D: Seoul Milk general milk.
VA, trans vaccenic acid; LA, linoleic acid; LNA, α-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acidVA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid
상기 표 8에서, 제품 A는 사료 급여를 통해 CLA 함량을 높인 우유이고, 제품 B는 유기농우유, 제품 C는 사료 유래 DHA 우유, 제품 D는 일반 우유이다.In Table 8, product A is milk with increased CLA content through feed feeding, product B is organic milk, product C is feed-derived DHA milk, and product D is general milk.
상기 표 8에서 확인할 수 있듯이, 제품 A는 CLA 함량이 높고, 오메가-3와 6의 비율은 1:3.7이지만, EPA와 DHA는 검출되지 않았다. 유기농우유 제품 B는 일반우유 보다 CLA 함량은 높지만, EPA, DHA는 검출되지 않았고, 오메가-3와 6의 비율은 1:7.6, 제품 C 우유는 DHA 함량 2.2 mg/100mL, CLA는 14.6 mg/100mL, 오메가-3와 6의 비율은 1:6.8, 제품 D는 CLA 함량 12.4 mg/100mL, 오메가-3와 6의 비율은 1:10.7이었다.As shown in Table 8, product A had a high CLA content and a ratio of omega-3 to 6 of 1: 3.7, but EPA and DHA were not detected. Organic milk product B had higher CLA content than normal milk, but EPA and DHA were not detected. The ratio of omega-3 to 6 was 1: 7.6, product C milk had a DHA content of 2.2 mg / 100 mL, CLA was 14.6 mg / , The ratio of omega-3 to 6 was 1: 6.8, that of product D was 12.4 mg / 100 mL of CLA, and the ratio of omega-3 to 6 was 1: 10.7.
따라서 기존의 시판 우유들은 VA, CLA, EPA, DHA를 모두 포함하면서 동시에 오메가 3, 6의 비율이 1:4 수준으로 유지하도록 구현하지 못하고 있음을 확인하였다. Therefore, it was confirmed that conventional commercial milk contains not only VA, CLA, EPA and DHA but also OMEGA 3 and 6 at a ratio of 1: 4.
반면, 본 발명에서 개발한 기능성 사료를 급여하여 얻은 우유는 VA, CLA, EPA, DHA를 모두 함유하고 있으며, 오메가-3와 6의 비율을 개선하여 건강한 기능성 지방산 조성을 갖는 우유 생산을 가능하게 해준다.On the other hand, the milk obtained by feeding the functional diet developed in the present invention contains all of VA, CLA, EPA and DHA and improves the ratio of omega-3 and 6, thereby enabling milk production with a healthy functional fatty acid composition.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.
Claims (3)
전체 사료 조성물에 대하여 지방산 공급원으로서 아마씨, 캐놀라씨 및 어유를 각각 1.80, 0.90 및 0.15 중량%, 부형제로서 수수, 기장 및 완두콩을 2 내지 4 중량%를 포함하는 것을 특징으로 하며,
기능성 지방산으로서 VA(trans-vaccenic acid), CLA(conjugated linoleic acid), EPA(Eicosapentaenoic acid), DHA(docosa hexaenoic acid)를 모두 포함하고 오메가-3와 6의 비율이 1:1 내지 1:4의 지방산 조성을 갖는 우유를 생산하기 위한 유우용 사료 조성물.In a dairy composition comprising a roughage, a by-product feed, a concentrated feed stuff, and an additive,
0.90 and 0.15% by weight of flaxseed, canola and fish oil as fatty acid sources for the whole feed composition, 2 to 4% by weight of millet and millet as the excipient,
(1) to (4), wherein the ratio of omega-3 to 6 is in the range of 1: 1 to 1: 4, and the ratio of omega-3 to omega A dairy cat composition for producing milk having a fatty acid composition.
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| KR20230126762A (en) | 2022-02-23 | 2023-08-31 | 김현수 | feed additives for dairy cow and manufacturing method thereof and production method of milk containing flavonoid |
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| KR102230081B1 (en) * | 2018-12-18 | 2021-03-19 | 이일준 | Feed composition |
| KR102076461B1 (en) * | 2019-11-15 | 2020-02-12 | 이일준 | Feed composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101166313B1 (en) | 2011-04-06 | 2012-07-17 | 박시내 | Compositions for adding to feeds regulated ratio of omega-6/omega-3 fatty acid and rearing methods of livestock using the same |
| WO2015016829A1 (en) | 2013-07-30 | 2015-02-05 | Benemilk Oy | Feed for lactating ruminants |
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| KR101166313B1 (en) | 2011-04-06 | 2012-07-17 | 박시내 | Compositions for adding to feeds regulated ratio of omega-6/omega-3 fatty acid and rearing methods of livestock using the same |
| WO2015016829A1 (en) | 2013-07-30 | 2015-02-05 | Benemilk Oy | Feed for lactating ruminants |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20230126762A (en) | 2022-02-23 | 2023-08-31 | 김현수 | feed additives for dairy cow and manufacturing method thereof and production method of milk containing flavonoid |
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