KR101782858B1 - Toothpaste composition comprising of medicinal plants - Google Patents

Abstract

The present invention relates to a dentifrice composition containing a medicinal plant extract and a preparation method thereof, and more particularly to a dentifrice composition having an effect of treating periodontal disease and removing bad breath including an extract of a soybean curd extract, a safflower extract and a safflower extract as active ingredients . The dentifrice compositions containing the extracts of the extracts of Aspergillus oryzae according to the present invention in a specific blending ratio can be used for a variety of dentifrice compositions such as Streptococcus mutans causative of cavities and porphyromonas gingivalis , It showed a strong antibacterial activity against pathogenic bacteria, especially octopus legs (Penthorum the extracts of the three kinds of chinense The dentifrice composition prepared by adding the Pursh extract has antibacterial activity against Helicobacter pylori pathogens and can be effectively used as a new functional dentifrice composition having periodontal disease improvement (or prevention) and bad breath removal effect have. The dentifrice composition of the present invention, which contains extracts derived therefrom, is a plant which is an edible plant, and which has no toxicity to octopus legumes, and has safety advantages even for long-term use.

Description

[0001] The present invention relates to a toothpaste composition containing a medicinal plant extract,

The present invention relates to a dentifrice composition containing a medicinal plant extract and a preparation method thereof, and more particularly to a dentifrice composition having an effect of treating periodontal disease and removing bad breath including an extract of a soybean curd extract, a safflower extract and a safflower extract as active ingredients .

In general, the oral cavity in the human body is a space in the mouth which is connected to the pharynx from the front lip to the rear mouth (mouth 峽). In addition to various functions related to food such as mastication, oral digestion and taste, As well as the endocrine action of salivary glands and respiration. In other words, it is the first gateway that all nutrients and atmospheric pathogens obtained through ingestion of food are invaded into the human body. It is used as a gateway to the function of chewing function and vocalization function, a tooth directly related to the aesthetic function of appearance, and gums supporting it, It is an important organ responsible for the health of the human body, such as the tongue, which is involved in function, is linked organically.

Periodontal tissues in the oral cavity are divided into cementum, gingiva (gum), periodontal ligament, and alveolar bone. To protect such periodontal tissues and gingiva, brushing after eating is very important. In this case, toothpaste and gaggle are used to remove acid, food waste, and harmful microorganisms in the food to prevent tooth decay and stomach upset and odor caused by harmful microorganisms . However, in the case of plaque occurrence, it is hard to prevent the occurrence of plaque by only gagulje, so brushing is always necessary.

A stigma is a very thin and transparent film consisting of bacteria, metabolites of bacteria or saliva of a living organism, which is a colorless bacterial film formed on the tooth surface. When the plaque is formed, the bacteria inside it multiply exponentially using the sugar that is supplied through the food consumed by the host organism. Bacteria in the dentition create toxins and free oxygen that breaks the gums, destroying the tissue surrounding the teeth. This causes irritation of the gums, redness of the gums, easy pouring and frequent bleeding. In addition, gaps between the gums and the teeth (gingival fissures) can open up a small pocket called a periodontal pouch. When the tooth builds up, the germs descend along the root surface to destroy the bone supporting the tooth, It is lost.

In addition, unremoved plaque is deposited with saliva and minerals such as calcium or phosphorus in the gingival fissure to create tartar. Calcite is also known as plaque and is a corrosive acid toxic substance that is an invisible transparent membrane and is the most direct cause of oral disease. Generating acid on the surface of teeth usually destroys the enamel, which is a hard part of the tooth, leading to calculus and extraction, and causing gum disease. In particular, oral bacteria such as Streptococcus mutans increase the fermentation of food to lower the pH of the mouth from neutral to slightly acidic, leading to halitosis, plaque formation and periodontal disease.

In an effort to prevent the formation of plaque, studies on dentifrice compositions using herbicides or compounds have been actively conducted. However, the conventional toothpaste uses a strong abrasive for removing the plaque, but it is not preferable to use a toothbrush containing a strong abrasive for those who have weak elderly people or those suffering from itigicin, and it is necessary to prevent various gum disease and periodontal disease, It is practically difficult to expect reduction of disease and healing effect.

Therefore, prevention of plaque formation by inhibiting the growth of Streptococcus mutans , which is the causative organism of plaque formation, and the prevention of formation of plaque by inhibiting bacteria that cause odor and living in the oral cavity, A solution is required.

Thus, the present inventors have superior antimicrobial activity of the cavity causing pathogen is Streptococcus mutans (Streptococcus mutans), taste causing organisms in Fort fatigue Pseudomonas long eccentricity less (porphyromonas gingivalis) and halitosis causing pathogen is Helicobacter pylori (Helicobacter pylori) pathogens As a result of searching for a medicinal plant which can be applied to toothpaste as a natural material having a specific composition ratio, a toothpaste composition containing a combination of a soybean curd extract, a safflower extract and a bamboo shoot extract at a specific blending ratio is added to Streptococcus mutans and Porphyromonas gingivalis pathogens ( Penthorum chinense) was applied to the three kinds of extracts, Pursh ) extract of the present invention has antibacterial activity against Helicobacter pylori. The present invention has been completed based on this finding.

Korean Patent Publication No. 10-2005-0020148

Accordingly, it is an object of the present invention to provide a dentifrice composition that is stable to human body and has excellent antimicrobial activity against bacteria causing cavities even when used for a long time by using a natural medicinal plant extract.

Another object of the present invention is to provide a method for manufacturing a dentifrice composition which is safe for human body and is excellent in antimicrobial activity against bacteria causing cavities even when it is used for a long time by using a natural medicinal plant extract.

In order to achieve the above-mentioned object of the present invention,

The present invention provides a dentifrice composition comprising a soybean curd extract, a safflower extract, and a safflower extract as an active ingredient.

In one embodiment of the present invention, the extract may be a hot-water extract.

In one embodiment of the present invention, the composition may include a soybean curd extract, a safflower extract and a safflower extract at a weight ratio of 1-5: 1-5: 1.

In one embodiment of the present invention, the composition may have antimicrobial activity against Streptococcus mutans , a causative organism causing cavities, and Porphyromonas gingivalis , a causative agent for aquatic plants .

In one embodiment of the present invention, the composition comprises an octopus leg ( Penthorum chinense Pursh ) extract.

In one embodiment of the present invention, the dentifrice composition may be in the form of a liquid, paste, or transparent gel type.

In addition, the present invention provides a method for producing a soybean curd, comprising the steps of: a) adding 5 to 10 times by weight of water to each of soybean curd, soybean curd, and bamboo shoots at a temperature of 50 to 60 DEG C to obtain a first- , Water was added to the vat in the first volume again, and the vial was cut at a temperature of 50-60 ° C for 7-8 hours to obtain a second volume of the extract except for the vat when the volume became one-third of the initial amount of the solution. Preparing an extract of the soybean curd extract, the extract of Aspergillus oryzae, and the extract of Aspergillus oryzae, respectively, by combining the extract and the secondary extract; b) mixing the soybean curd extract, the safflower extract, and the shoemaker extract; And c) adding a salt, a wetting agent and a sweetening agent to the mixture to dissolve the mixture, adding a thickener, an abrasive, and a foaming agent to the mixture, and finally adding a flavoring agent.

The dentifrice compositions containing the extracts of the extracts of Aspergillus oryzae according to the present invention in a specific blending ratio can be used for a variety of dentifrice compositions such as Streptococcus mutans causative of cavities and porphyromonas gingivalis , It showed a strong antibacterial activity against pathogenic bacteria, especially octopus legs (Penthorum the extracts of the three kinds of chinense The dentifrice composition prepared by adding the Pursh extract has antibacterial activity against Helicobacter pylori pathogens and can be effectively used as a new functional dentifrice composition having periodontal disease improvement (or prevention) and bad breath removal effect have. The dentifrice composition of the present invention, which contains extracts derived therefrom, is a plant which is an edible plant, and which has no toxicity to octopus legumes, and has safety advantages even for long-term use.

Fig. 1 is a photograph showing the soybean extract of the present invention (soybean concentrate).
FIG. 2 is a photograph showing the extract of Liliaceae (Liliaceae) concentrate of the present invention.
Fig. 3 is a photograph showing the spear extract (spruce concentrate) of the present invention.
4 is a photograph showing the dentifrice composition of the present invention.

The present invention is characterized by providing a dentifrice composition comprising an extract of the extracts of Aspergillus, Aspergillus oryzae as an active ingredient.

As used herein, the term " soybean & quot ; gladiata DC, a royal family crop that has been used only as a medicinal plant and food in the royal family of the Koryo Dynasty, and is a native plant that has been banned for cultivation due to the Koryo era. Soy beans are called pods or doodles because their shape is the same as they are bent pods. The shape and characteristics of soybean is annual plant, and the leaf is a compound leaf composed of three long chicken - egg - shaped leaflets and has a triple - lapped form. A light red or colored flower blooms and the length of the belly of the seed is half the length of the seed. There are three kinds of cultivars with white lips of white flowers, red lips of white flowers, and red lips of red flowers. It is a crop with high resistance to one foot, strong acidity, sandy soil and shady places. The distribution of soybean is widely distributed in tropical Asia, Africa, and India and is mainly cultivated within 1000m above sea level, and cultivated in Southeast Asia and Korea. The major constituents of soybean are urea, hemagglutinin, cannabarin, protein, and oil, and hemagglutinin is composed of various globulins such as Concanavalin A. This component is known to have a specific action on tumor cells. In addition, there are glucosidase, arginase, and vitamin B group, vitamin C, niacin is 3 to 5 times more than other soybean and crops.

"Curly dock" referred to herein is the scientific name Rumex crispus L. It is called Ladybird, Ladybird, Songguji, Opposite Ear, etc. It grows commonly in the wet areas of the fields and mountain slopes of the country. It has bloomed roots in the ground, and the coarse stems grow up to 30-80cm There are many vertical lines, and the backlight turns on the green background. By the time the young leaves grow up, they look tall and tiny. In spring, young seeds are eaten as herbs and rooted as medicine. Examples of such molluscs include ascorbic acid, crysopanol, emodin, anthraquinone derivatives, calcium monacalcium, essential oils, oxymetalanthroquinone, chrysopanic acid, anthraglycoside and the like.

"Jokduripul 'scientific names mentioned herein are the Asarum It is called sieboldii. It is called a perennial plant. It is called a tsukuri, a foxbush, and a rootstock with many irregular nodes spreads to the side, and its roots descend. Two leaves from the roots face each other and spread. It grows about 20cm in height with the name of the leaf which is similar to the stem-shaped leaf. The spicy root has a lot of nodes, a lot of beard roots and meat quality. The active ingredients include Methyleugenol, Asarylketone, Safrole, 1,8-cineol, Asarinin, Eucavone, etc. , p56-p57, Choi, Soon Chan, Knowledge Center, 2015).

The present inventors have searched for a medicinal plant which can be applied to toothpaste as a natural material having excellent antimicrobial activity against harmful bacteria in the oral cavity. As a result, a toothpaste composition containing a soybean extract, a safflower extract and a safflower extract at a specific blending ratio, Coxs mutans and Porphyromonas gingivalis were identified for the first time.

Therefore, the composition of the present invention comprising the extracts of Aspergillus, Aspergillus oryzae as an active ingredient can be usefully used as a toothpaste material.

The extract according to the present invention can be obtained by extracting and isolating from nature using extraction and isolation methods known in the art, and the 'extract' as defined in the present invention can be obtained by using an appropriate solvent, For example, crude extract, polar solvent-soluble extract or non-polar solvent-soluble extract of the medicinal plant.

As an appropriate solvent for extracting the extract from the soybean sprout, safflower, and shoemaker, any organic solvent that is pharmaceutically acceptable may be used, and water or an organic solvent may be used. For example, Alcohol having 1 to 4 carbon atoms, acetone, ether, or the like containing water, purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. Preferably, water is used.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. The method for producing the medicinal plant extract of the present invention is not limited, and any known method can be used.

For example, the medicinal plant extract contained in the composition of the present invention may be prepared into a powdery state by an additional process such as vacuum distillation, freeze-drying, or spray drying, have. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, the medicinal plant extract in the present invention is a concept including all of the extract, fraction and purified product obtained in each step of extraction, fractionation or purification, a diluted solution thereof, a concentrate or a dried product.

In the present invention, all of the three medicines (eg, soybean sprouts, shrubs, and shrubs) can be extracted at once, or they can be extracted and mixed. However, efficient extraction of the active ingredient and extraction of subcomponents during mixed extraction of medicinal plants It is preferable to use them separately and then mix them together.

The method for producing the medicinal plant extract according to one embodiment of the present invention will be described in more detail as follows.

5-10 times the weight of water was added to each of the soybean curd, shrubs and shrimp, and the mixture was cooled at a temperature of 50-60 ° C. When the amount of the aqueous solution became 1/3, the extract solution except the mulch was firstly obtained, After the addition at the initial dose, the extracts can be prepared at 50-60 ° C until the amount of aqueous solution is reduced to 1/3, respectively, to produce each of the extracts of Aspergillus oryzae, Aspergillus oryzae.

It is possible to extract all three dried drugs (soybean sprout, spruce sprout, and spruce sprout) at once, but they can be extracted and mixed after they are separately extracted. However, efficient extraction of active ingredients and extraction of subcomponents during mixed extraction of medicinal plants are minimized To do this, individual extraction and mixing methods were followed.

Each of the soybean curd extract, the safflower extract and the bamboo shoot extract of the present invention has an excellent antimicrobial activity against Streptococcus mutans . In the case of a composition prepared by mixing the medicinal plant extract at a specific ratio, (Porphyromonas gingivalis ), in addition to Pseudomonas sp .

In one embodiment of the present invention, the composition may contain the soybean curd extract, the safflower extract and the safflower extract at a weight ratio of 1-5: 1-5: 1, and preferably the soybean curd extract, the safflower extract, The extract may be contained in a weight ratio of 5: 5: 1.

Streptococcus mutans referred to herein is a bacterial species present in the oral cavity of a normal person and is associated with an early stage of enamel damage and is a major causative organism of the tooth cavity. Dissolve sugar to synthesize insoluble dextran. Dextran aggregates oral bacteria, including mutans, to form plaques, and enamel demineralization and cavities are generated by the metabolites (acid) of pragan.

The term " porphyromonas gingivalis " referred to herein is a gram-negative rod-shaped anaerobic pathogenic bacterium that causes oral diseases such as periodontitis.

Therefore, the composition comprising the extracts of Aspergillus oryzae, the extract of Aspergillus oryzae and the extract of Aspergillus oryzae as an active ingredient of the present invention can be used as an effective ingredient of the present invention, for example, Streptococcus mutans which is a causative agent of cavities, porphyromonas gingivalis . The composition of the present invention containing the active ingredient can be usefully used as a functional dentifrice composition having an effect of improving (or preventing) periodontal disease and removing bad breath.

The extracts of Seaweed Extract, Rhododendron syrup extract and Rhododendron syrup extract of the present invention may be contained in an amount of 0.00001 to 50.0% by weight based on the total weight of the dentifrice composition and may be contained in the composition at a concentration of 1 μg / ml to 100,000 μg / ml.

In one embodiment of the present invention, the dentifrice composition may further comprise an extract of Penthorum chinense Pursh as an effective ingredient in addition to the extracts of soybean curd extract, saxifrage extract and bamboo shoot extract.

The term "octopus bridge" referred to in this specification refers to Penthorum chinense Pursh is a perennial plant of the stems. It is used as a medicinal material for the blood-sucking, rowing, uterine bleeding, menstrual abolition, species, No studies have been reported.

The present inventors have found that when octopus legs extracts having the above-described characteristics are mixed with the medicinal plant extracts (soybean curd extract, shrubs extract and bamboo shoot extract) to prepare dentifrice compositions, excellent antimicrobial activity against Helicobacter pylori, Of the total population.

The extract of octopus leg of the present invention is prepared by extracting octopus leg dry powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; And lyophilizing and pulverizing the concentrated filtrate.

The dentifrice composition of the present invention can be used as a dentifrice composition in addition to the above-mentioned active ingredients (soybean extract, safflower extract, bamboo shoot extract, octopus leg extract), sun salt (or bamboo salt), wetting agent, sweetener, thickener, abrasive, Preservatives, preservatives, pigments, and the like.

The term "sun-salt" referred to in the present specification is made by forming salt crystals of a cubic body by drawing a salt component containing 2-3% in seawater into a salt and spontaneously evaporating moisture with sunlight and wind. The main component of the salt is sodium chloride (Nacl), which contains minerals such as calcium, magnesium and potassium.

The term " wetting agent " as used herein refers to a component that is employed for the purpose of preventing the toothpaste from contacting with the air to solidify the toothpick in order to impart moisture and cream to the toothpaste. Examples thereof include D-sorbitol solution, glycerin, polyethylene glycol, and the like, and can be appropriately selected by a person skilled in the art.

As used herein, the term " thickening agent " refers to a component employed for the purpose of preventing the separation of a solid component and a liquid component of a toothpaste. Examples thereof include sodium carboxymethylcellulose or hydrated silicic acid, and a person skilled in the art can appropriately select them.

As used herein, the term " sweetener " refers to a component that is employed for the purpose of imparting a certain aesthetics to a toothpaste and imparting a cooling sensation and the like. Examples thereof include xylitol, steviol glycoside, and saccarin, and can be appropriately selected by a person skilled in the art.

The term "abrasive" as used herein refers to a component that acts to remove dental plaque (which is only removed by abrasion with a sticky polysaccharide film) and tartar attached to the teeth. Examples thereof include dental type silica, hydrated silicon dioxide, colloidal silicon dioxide, precipitated silica, silica gel, zirconium silicate, tricalcium phosphate, anhydrous calcium phosphate, calcium monohydrogenphosphate, calcium monohydrogenphosphate anhydrous, silicon dioxide, hydrated alumina, Precipitated calcium carbonate, light calcium carbonate, heavy calcium carbonate, sodium pyrophosphate, insoluble metaphosphate and aluminum silicate, and the like can be appropriately selected by a person skilled in the art.

The 'foaming agent' referred to in the present specification supplements the cleaning action of the abrasive, penetrates the hard-to-reach areas where the toothbrush is hard to reach, as well as enhances the feeling of brushing by generating air bubbles. And the interfacial tension is decreased, so that the foreign substances in the oral cavity easily drop. Examples thereof include anionic surfactants such as sodium laurylsulfate and sodium alkylsulfate, as well as nonionic surfactants such as polyoxyethylene, polyoxypropylene, copolymers, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid, alkanolamide Fatty acid esters, sucrose fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters and polyoxyethylene castor oil hardened oil derivatives, and the like can be appropriately selected by those skilled in the art.

As used herein, the term " flavoring agent " refers to a component that is employed for the purpose of cleansing the mouth, giving a refreshing feeling, and suppressing the unpleasant odor in the mouth. Examples include Peppermint Oil, Spearmint Oil, Orange Oil, Lime Oil, Lemon Oil, L-Menthol, Fruit Flavor, Cool Mint Flavor (HF-61399) or Combination Flavor (Apple Flavor, HF-30630) May be appropriately selected by a person skilled in the art.

The dentifrice composition of the present invention comprising the above-mentioned active ingredients and additives may be a liquid, paste or transparent gel type formulation, but any type of formulation can be used as long as it can function as a dentifrice composition. But is not limited to.

The present invention also relates to a method for producing a soybean curd, comprising the steps of: a) adding 5 to 10 times by weight of water to each of soybean curd, soybean curd and shiitake, respectively, at a temperature of 50 to 60 DEG C to obtain a first- , Water was added to the vat in the first volume again, and the vial was cut at a temperature of 50-60 ° C for 7-8 hours to obtain a second volume of the extract except for the vat when the volume became one-third of the initial amount of the solution. Preparing an extract of the soybean curd extract, the extract of Aspergillus oryzae, and the extract of Aspergillus oryzae, respectively, by combining the extract and the secondary extract; b) mixing the soybean curd extract, the safflower extract, and the shoemaker extract; And c) adding a salt, a wetting agent and a sweetening agent to the mixture to dissolve the mixture, adding a thickener, an abrasive, and a foaming agent to the mixture, and finally adding a flavoring agent.

The step a) of the present invention is a step of preparing each of the extracts of Artemisia soiulifolia, Rhododendron japonica extract and Liliaceae. In detail, 5-10 times of water is added to each of soybean curd, When the volume was reduced to 1/3, the extract solution was firstly obtained except for the mackerel. Then, water was added to the mackerel again for the first time, and then the mixture was suspended at 50-60 ° C for 7-8 hours. , And then adding the primary extract and the secondary extract obtained in the above step to each other to prepare the extracts of Artemisia bean extract, Aspergillus oryzae and Aspergillus oryzae respectively.

The step b) of the present invention is a step of preparing a mixed solution by mixing the extracts of Artemisia princescens, Rhododendron syrup extract, and Syrup extract. Specifically, the step of preparing a mixture solution of the extracts of Soybean curd extract, Rhododendron syrup extract and Syrup extract, 1-5: 1-5: 1.

In step b) of the present invention, the soybean curd extract, the safflower extract, and the safflower extract prepared through step a) are mixed at a weight ratio of 1-5: 1-5: 1, And may be made into a mixture through a process of breaking until the amount of the aqueous solution is 1/2. This is because it may be inconvenient when the respective extracts are mixed and used, so that they can be manufactured in a mixed form according to their preferences and used as needed. At this time, it is preferable that the specific gravity of the mixture is 1.1 or less. If the specific gravity exceeds 1.1, the effective ingredient of the medicinal plant is destroyed. When the mixture is mixed with the tooth paste raw material, It should be avoided.

The step c) of the present invention is a step of adding a salt, a wetting agent, a sweetening agent, a thickener, an abrasive, a foaming agent and a flavoring agent to the mixture prepared through the step b) A salt, a wetting agent and a sweetening agent are added and dissolved, and then a thickener, an abrasive and a foaming agent are added and mixed, and finally, a flavoring agent is added.

In one embodiment of the present invention, the wetting agent may be glycerin; The sweetener may be xylitol and sorbitol; The thickener may be carboxymethylcellulose; Silicon dioxide may be used as the abrasive, and sodium lauryl sulfate or sodium lauroyl sarcosinate may be used as the foaming agent, but the type thereof is not particularly limited .

In one embodiment of the present invention, each of the soybean curd extract, the safflower extract, and the shoemoleberry extract as the medicinal plant extract may be contained in an amount of 1 to 10 parts by weight based on 100 parts by weight of the toothpaste composition; The sun salt may be contained in an amount of 1-20 parts by weight based on 100 parts by weight of the toothpaste composition; The wetting agent may be present in an amount of 1-10 parts by weight based on 100 parts by weight of the toothpaste composition; The sweetener may be included in an amount of 30 to 70 parts by weight based on 100 parts by weight of the total dentifrice composition; The thickening agent may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the total dentifrice composition; The abrasive may be included in an amount of 5 to 15 parts by weight based on 100 parts by weight of the toothpaste composition; The foaming agent may be included in an amount of 1 to 10 parts by weight based on 100 parts by weight of the toothpaste composition; The flavoring agent may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the total dentifrice composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Preparation of medicinal plant extract

<1-1> Preparation of Soybean Extract

Soybean (pods) used in the present invention was collected in October 2015 from Ewha Farm, Mungyeong, and cleaned with water and used. The soybeans were cut in half and the pods were cut to a size of about 1 to 2 mm using a tablet for medicine and then dried. The drying process can be carried out for 3 to 7 days on shrubs, or dried for 10-14 hours using a food drier until the moisture content is within 10%.

5 kg of water was added to 1 kg of soybean koji prepared by the above procedure, and water was soaked for 1 hour, so that the water was completely immersed in soybean koji. Then, it was cut at 50-60 ° C for 7-8 hours, , The extract solution was firstly obtained, and 5 kg of water was added again to the mackerel, which was then terminated at 50-60 ° C for 7-8 hours. When the volume became 1/3 of the initial amount of the solution, the extract solution except the mackerel Lt; / RTI &gt; The thus obtained primary extract and secondary extract were combined to prepare a soybean curd extract.

<1-2> Preparation of Syrup extract

The spider used in the present invention was directly collected at Seonggi Mountain in Cheonan Province in August 2015. The roots were washed with water and cleaned. The roots were washed with water and then cut to a size of 1-2 mm. Then, the roots were washed with water, -1 &gt;.

5 kg of water was added to 1 kg of syrup prepared by the above procedure, and water was soaked in the syringe for 1 hour. After the water was completely immersed in the syringe, it was withdrawn at 50-60 ° C for 7-8 hours, / 3, the extract was firstly obtained except for the gherkin, and 5 kg of water was added again to the gherkin and the mixture was cut at 50-60 ° C for 7 to 8 hours. When the volume of the solution reached 1/3 of the initial amount of the solution, Secondary extracts were obtained. The first extract and the second extract obtained as described above were combined to prepare a safflower extract.

<1-3> Bamboo shoot  Preparation of extract

The ladybug used in the present invention was directly collected at Taejo Mountain, Cheonan Province in September 2015. The shoehorns were cleaned with water and then prepared in a size of 3-4 mm, followed by drying in the same manner as in Example <1-1>.

5 kg of water was added to 1 kg of the prepared shoe lilies prepared by the above process, and water was soaked for 1 hour, so that the water was completely immersed in the shrimp, and then the shrimp was cut at 50-60 ° C for 7-8 hours, , The extract solution was firstly obtained, and 5 kg of water was added again to the mackerel, which was then terminated at 50-60 ° C for 7-8 hours. When the volume became 1/3 of the initial amount of the solution, the extract solution except the mackerel Lt; / RTI &gt; The first extract and the second extract thus obtained were combined to produce an extract of Lycopersicon esculentum.

&Lt; 1-4 > Production of a counter electrode extract

The upper part of the counter pole ( Euphorbia pekinensis RUPR . ) Used in the present invention was collected in May, 2014 from Seodong-dong Suwon and used as a sample. The ground part of the recovered main pole was washed, dried and pulverized into powders. The powder was extracted with methanol using an accelerated solvent extractor (ASE) at 50 ° C. The extract was filtered and then concentrated under reduced pressure using a rotary evaporator To remove the solvent. The amount of the extract obtained from 100 g of the groundwater phase powder was 16 g.

<1-5> Preparation of chrysanthemum extract

Alveolar used in the present invention (Physalis There is alkekengi . The top part of the francheti (Mast.) Hort was collected in July 2014 in Seodong-dong Suwon and used as a sample. The extracted acicular ground part was washed, dried and then pulverized into powders. The powder was extracted with methanol using an accelerated solvent extractor (ASE) at 50 ° C. The extract was filtered and then concentrated under reduced pressure using a rotary evaporator To remove the solvent. (China) 10 g of the extract obtained from 100 g of the chrysanthemum ground powder was 10 g.

<1-6> Preparation of Octopus Leg Extract

The octopus legs ( Penthorum chinense Pursh ) was collected in July 2014 in Seodong-dong Suwon and used as a sample. The ground portion of the harvested octopus legs was washed, dried and pulverized to powder. The powder was extracted with ethanol using an accelerated solvent extractor (ASE) at 85 ° C. The extract was filtered and concentrated under reduced pressure using a rotary evaporator to obtain a solvent Respectively. The extract obtained from 165g of octopus leg root powder was 28g.

Each of the extracts thus obtained was used as a sample for the following experiment while being stored at 4 ° C in a refrigerator. The extracts of soybean curd, rosemary extract and bamboo shoots were prepared in liquid form; The major pole extract, kelp extract and octopus leg extract were prepared as powder.

< Example  2>

Antibacterial effect of single medicinal plant extracts

The present inventors have examined the antibacterial effect of each of the medicinal plant extracts prepared in Example 1 above.

For the antimicrobial effect test, the inhibitory activity against Streptococcus mutans was examined.

The antimicrobial effect was evaluated by disk diffusion method. That is, the strain was cultured in TBS medium at 37 ° C until the measured value was 0.5 at OD 600 nm. 100 μl of culture at a concentration of about 10 ⁴ to 10 ⁵ CFU / ml was plated on MHA agar medium. 1 mg and 5 mg of each extract was added to the disk (diameter 6 mm) and the disk was placed on the above MHA medium. After incubation at 37 ° C for 24 hours, the diameter of the inhibition zone was measured in the solid medium in which the microorganism was cultured. The experiment was repeated three times and the average value was shown.

Antimicrobial Effect of Streptococcus mutans on Medicinal Plant Extract Strain density Disk radius (mm) Soybean
extract Shout
extract Bamboo shoot extract Antipode
extract ground cherry
extract Octopus Leg Extract Streptococcus mutans 1mg disk 13.0 10.0 11.0 - - - 5mg disk 9.0 7.0 8.0 - - -

< Example  3>

Manufacture of toothpaste compositions comprising medicinal plant extracts

The solar salt used in the following experiment was purchased from Bujang Gomso Tidal Flat, Jeonbuk Province; Glycerine was purchased from LG Household & Health Care; Xylitol was purchased from Borac; Sorvitol was purchased from Kwangwang Chemical; thickener (CMC-Na) was purchased from Borac; silicon dioxide was purchased from space industry; Sodium lauroyl sarcosinate (SLS; sodium lauryl sulfate / Miami L95) was purchased from Miwon.

The present inventors conducted an experiment to obtain mixing ratios having the optimum effects for preventing periodontal disease and suppressing plaque by mixing the extracts of the soybean curd extract, the extract of Aspergillus oryzae and the extract of Aspergillus oryzae, prepared through the <Example 1> as a medicinal plant.

In detail, the soybean curd extract, the safflower extract, and the safflower extract prepared through Example 1 of the present invention were mixed at a weight ratio shown in Table 2 below. Then, the mixture was stirred with gentle saline, glycerin, xylitol and sorbitol in order to dissolve them all at once. The final dentifrice composition was prepared by adding carboxymethyl cellulose, silicon dioxide, foaming agent and finally adding pepper powder. The weight ratios of the constituents added are shown in Table 2 below.

The composition of the dentifrice composition and its blending ratio ingredient Example T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 Soybean extract One 3 5 10 7 10 One One One One Sorghum extract One 3 5 10 7 10 One One 3 5 Bamboo shoot extract One One One 2 One One 3 5 3 5 Sun salt 8 8 8 8 8 8 8 8 8 8 Silicon dioxide 16 16 16 16 16 16 16 16 16 16 glycerin 5 5 5 5 5 5 5 5 5 5 Xylitol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Sorbitol 63.2 59.2 55.2 44.2 51.2 45.2 61.2 59.2 59.2 55.2 Foaming agent 3 3 3 3 3 3 3 3 3 3 Carboxymethylcellulose 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Park Hahn One One One One One One One One One One Total amount 100 100 100 100 100 100 100 100 100 100 Foaming agent: Sodium Lauryl Sulfate and Sodium lauroyl sarcosinate were mixed in a ratio of 3: 1

< Example  4>

The dentifrice composition of the present invention Feeling  evaluation

<4-1> For people without gum disease Feeling  evaluation

Ten toothpaste samples of Examples T1 to T10 of the present invention were used for 50 persons (30 males and 20 females) aged 40 years or over without periodontal disease, and the following items were examined and the results Are shown in the following table.

The evaluation was scored on a 5-point scale and was rated as 5-excellent, 4-good, 3-normal, 2-poor, and 1-very bad.

Usability evaluation according to composition ratio (weight ratio) of dentifrice composition Example Appearance Viscosity flavor Flavor Bubble power Cleaning power Recuperation Total score T1 5 4 4 4 5 4 3 29 T2 4 4 4 4 5 4 4 29 T3 4 4 4 4 4 4 4 28 T4 4 4 3 3 4 4 4 26 T5 One 2 2 2 One 2 3 13 T6 One 2 2 One One 2 2 11 T7 2 2 One 2 2 3 3 15 T8 One 2 2 One 4 2 2 14 T9 One 3 One 3 2 3 2 15 T10 One 2 2 2 4 One 3 15

As a result, as shown in Table 3, it can be seen that the overall satisfaction is higher than that of T5 to T10 in Examples T1 to T4, and that the total score difference is nearly double.

<4-2> Measurement of gum treatment and bad breath removal effect on subjects with gum disease

100 subjects with periodontal disease, gingival bleeding, gingivitis, gingival diseases and bad breath were selected, and then randomly divided into 10 groups of 10 persons. 10 subjects divided into 10 groups were allowed to use the toothpaste prepared in Examples T1 to T10 three times a day for 4 weeks.

The gingival disease therapeutic effect was measured by using the toothpaste manufactured in Examples T1 to T10 for 4 weeks for the subjects of each experimental group, and then the degree of treatment satisfaction for gum disease such as periodontitis, gum bleeding, After the evaluation, the average value was measured. The evaluation was on a scale of 5 points. One point: Very unsatisfied, 2 points: Dissatisfied, 3 points: Average, 4 points: Satisfaction, 5 points: Very satisfied.

On the other hand, the measurement of the odor elimination effect was carried out by measuring the degree of bad breath of the subjects of each experimental group before (before the experiment) and after using the toothpaste (after the experiment and after the rinse) Halimeter), and the mean value was compared.

Effect of gum disease treatment and odor removal according to composition ratio of dentifrice composition division Item Example T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 Gum disease Periodontitis 3.7 3.8 4.2 4.5 2.8 2.2 2.5 2.2 2.8 3.0 Bleeding gums 4.0 4.0 4.7 4.5 3.0 3.2 2.8 2.8 3.2 2.5 Igarashim 3.8 4.0 3.7 4.0 2.8 2.2 2.2 2.8 2.0 2.2 Bad breath Just before the experiment 200.4 207.5 210.2 201.7 205.0 204.5 210.5 200.0 210.2 205.1 After 4 weeks 110.1 108.5 113.8 105.5 135.0 138.5 140.8 132.2 142.5 135.2

As a result, as shown in Table 4, it was confirmed that T1-T4 was superior to T5-T10 in the treatment of gum disease and bad breath removal.

The inventors of the present invention have determined that the above-mentioned effects are attributable to the antibacterial and anti-inflammatory effects through inhibition of the activity of the causative bacteria. In order to see if there is actually such an effect, an additional antibacterial effect was experimented.

< Example  5>

The antimicrobial effect of the dentifrice composition of the present invention

In order to test the antibacterial effect of the dentifrice composition prepared in Example 3, Streptococcus mutans , porphyromonas gangrene, gingivalis ) and Helicobacter pylori ( Helicobacter pylori ).

First, Streptococcus mutans , a causative organism causing cavities, and porphyromonas ( porphyromonas) gingivalis ) and the minimum inhibitory concentration (MIC) for Helicobacter pylori , a causative organism causing bad breath. The measuring method was measured using a broth dilution method. First, the dentifrice composition was diluted 100 times with purified water, diluted twice to 28 with 5 ml of liquid medium, and then inoculated with the bacteria. The cells were cultured in an environment of 37 ° C and 5% CO ₂ for 3 hours, and the temperature at which no growth of the strain was observed was determined as MIC. The MIC determination results are shown in the following table.

Antibacterial effect on oral bacteria (μg / ml) Strain Example T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 ST 1.8 1.2 1.0 1.0 3.2 3.8 4.4 3.4 4.0 3.2 PG 2.0 2.2 1.5 1.8 3.8 3.4 3.4 4.2 4.0 4.2 HY - - - - - - - - - - ST: Streptococcus mutans , PG: porphyromonas gingivalis , HP: Helicobacter pylori

As a result, as shown in Table 5, in the case of the T1-T4 in comparison to T5-T10 caries causing organisms of streptococcus mutans (Streptococcus mutans), taste causing organisms in long Pseudomonas formate fatigue eccentricity Lees (porphyromonas gingivalis) It was confirmed that the antimicrobial activity was remarkably excellent. On the other hand, there was little antimicrobial activity against Helicobacter pylori .

< Example  6>

Manufacture of toothpaste compositions with additional medicinal plant extracts

The inventor of the present invention intends to find a material capable of enhancing the effectiveness of the periodontal disease-suppressing effect by additionally adding to the dentifrice composition of the present invention prepared through the above Example 3. As a candidate group, the antipark extract prepared in Example 1 (Example <1-5>) and octopus leg extract (Example <1-6>) were used.

In detail, soybean curd extract, sardine extract, bamboo shoot extract, Diptera extract, corn root extract and octopus leg extract prepared through Example 1 of the present invention were mixed at the weight ratios shown below. Then, the mixture was stirred at a weight ratio shown in the following table, and the mixture was stirred so that all of the salts were dissolved. Carboxymethylcellulose, silicon dioxide and foaming agent were again added thereto in the weight ratios shown in the following table, and finally, a peppermint was added to prepare a final dentifrice composition.

The composition of the dentifrice composition added with the additional medicinal plant extract and the blending ratio thereof ingredient Example T11 T12 T13 T14 T15 T16 Soybean extract One 3 5 One 3 5 Sorghum extract One 3 5 One 3 5 Jujube grass extract One One One One One One Antipark extract 0.1 - - 0.2 - - Kelp extract - 0.1 - - 0.2 - Octopus Leg Extract - - 0.1 - - 0.2 Sun salt 8 8 8 8 8 8 Silicon dioxide 16 16 16 16 16 16 glycerin 5 5 5 5 5 5 Xylitol 0.3 0.3 0.3 0.3 0.3 0.3 Sorbitol 62.2 58.2 54.2 61.2 57.2 53.2 Foaming agent 3 3 3 3 3 3 Carboxymethylcellulose 0.5 0.5 0.5 0.5 0.5 0.5 Park Hahn One One One One One One Total amount 100 100 100 100 100 100 Foaming agent: Sodium Lauryl Sulfate and Sodium lauroyl sarcosinate were mixed in a ratio of 3: 1

< Example  7>

Of a dentifrice composition to which an additional medicinal plant extract has been added Feeling  evaluation

<7-1> For people without gum disease Feeling  evaluation

Sixty toothpastes of T11-T16 of the present invention were used in 50 persons (40 males and 20 females) aged 40 years or over without periodontal disease, and the following items were examined and the results of the mean values were shown Respectively.

The evaluation was scored on a 5-point scale and was rated as 5-excellent, 4-good, 3-normal, 2-poor, and 1-very bad.

Evaluation of usability according to composition ratio (weight ratio) of the dentifrice composition added with additional medicinal plant extract Example Appearance Viscosity flavor Flavor Bubble power Cleaning power Recuperation Total score T11 5 4 4 4 5 4 4 30 T12 4 4 4 4 5 4 4 29 T13 4 4 4 4 5 4 4 29 T14 4 4 3 3 5 4 4 27 T15 5 4 4 3 5 4 5 30 T16 4 4 4 3 5 4 5 29

As a result, as shown in Table 7, the overall satisfaction was high in T11 to T16, and there was not a large difference in feeling among the experimental groups.

<7-2> Measuring gum treatment and bad breath removal effect on subjects with gum disease

Sixty subjects with gum disease and bad breath, such as periodontitis, gum bleeding, and irritable bowel syndrome, were selected and randomly divided into six groups of ten. Ten subjects who were divided into six groups were allowed to use toothpaste manufactured for four weeks three times a day.

The gingival disease treatment effect was measured by using the toothpaste manufactured by T11-T16 for 4 weeks for subjects in each experimental group, and then the degree of treatment satisfaction with gum disease such as periodontitis, gum bleeding and iridescence was evaluated on a 5 point scale Then, the average value was measured. The evaluation was on a scale of 5 points. One point: Very unsatisfied, 2 points: Dissatisfied, 3 points: Average, 4 points: Satisfaction, 5 points: Very satisfied.

On the other hand, the odor elimination effect was measured by measuring the degree of bad breath of the subjects of each experimental group before (before and after the experiment) and after using the toothpaste manufactured by T11-T16 (before and after the experiment) Halimeter), and the mean value was compared.

Effect of treatment of periodontal disease and removal of bad breath according to the composition ratio of the additional dentifrice composition added with medicinal plant extract division Item Example T11 T12 T13 T14 T15 T16 Gum disease Periodontitis 4.0 4.1 4.8 4.0 3.8 4.8 Bleeding gums 3.8 4.0 4.8 3.7 4.0 4.5 Igarashim 3.8 3.7 4.0 4.0 3.8 4.5 Bad breath Just before the experiment 201.2 204.7 198.8 200.2 202.0 195.5 After 4 weeks 103.5 108.4 85.8 108.5 110.5 85.5

As a result, as shown in Table 8, it was confirmed that the treatment for gum disease and the removal of bad breath were further enhanced in T13 and T16, which further included octopus legs extract.

The inventors of the present invention have determined that the above-mentioned effects are attributable to the antibacterial and anti-inflammatory effects through inhibition of the activity of the causative bacteria. In order to see if there is actually such an effect, an additional antibacterial effect was experimented.

< Example  8>

The antimicrobial effect of the dentifrice composition of the present invention added with an additional medicinal plant extract

In order to test the antimicrobial effect of the dentifrice composition prepared in Example 6, Streptococcus mutans , porphyromonas gangrene, gingivalis ) and Helicobacter pylori ( Helicobacter pylori ).

The experimental procedure was the same as in Example 5, and the MIC determination results are shown in the following table.

Antibacterial effect on oral bacteria (μg / ml) Strain Example T11 T12 T13 T14 T15 T16 ST 1.2 1.2 0.3 1.0 1.2 0.2 PG 1.8 2.0 0.5 1.0 1.8 0.5 HY - - 2.4 - - 2.0 ST: Streptococcus mutans , PG: porphyromonas gingivalis , HP: Helicobacter pylori

As a result, as shown in Table 9, in the case of T13 and T16, which further included octopus legs extract, Streptococcus mutans , a causative organism causing cavities, and Porphyromonas long, The antimicrobial activity against porphyromonas gingivalis was remarkably excellent, and the antimicrobial activity against Helicobacter pylori , which is a cause of bad breath, was also excellent.

Overall jakdukong extract, ivy extract, and when the sound of the dentifrice composition comprising the extract in a specific mixing ratio jokduripul periodontal diseases treatment (or prevention) effects were confirmed through experiments the superior, especially octopus legs (Penthorum chinense Pursh extract of the present invention has an excellent inhibitory activity on the causative bacteria of tooth decay and also has an inhibitory activity on Helicobacter pylori as a bad breath inducing microorganism.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

As toothpaste compositions,
Wherein said composition comprises as an active ingredient soybean curd extract, safflower extract, bamboo shoot extract and octopus leg extract,
The extracts of the above mentioned soybean curd extract, safflower extract, bamboo shoot extract and octopus leg are contained in a weight ratio of 1-5: 1-5: 1: 0.1-0.2,
The above composition has antibacterial activity against all bacteria against Streptococcus mutans which is a causative organism causing cavities, Porphyromonas gingivalis which is a causative agent of the taste, and Helicobacter pylori which is a cause of bad breath by Helicobacter pylori And a dentifrice composition. The method according to claim 1,
Wherein the extract is a hot-water extract. delete delete delete 3. The method according to claim 1 or 2,
Wherein the dentifrice composition is a liquid, paste, or transparent gel type formulation. a) 5-10 times water by weight is added to each of alfalfa, sorghum and bamboo shoots at a temperature of 50-60 &lt; [deg.] &gt; C, and when the volume becomes 1/3 of the volume, the extract is firstly obtained except for the gherkin, Was added for the first time, and the mixture was cut at a temperature of 50-60 ° C for 7-8 hours. When the volume became one-third of the initial amount of the solution, the extract solution except the mulch was secondarily obtained. Preparing an extract of Octopus legume by adding ethanol to the dry powder of the octopus legs;
b) mixing the four kinds of the extracts of the above mentioned soybean curd extract, Sepharose extract, Leaf extract, and Octopus legs at a weight ratio of 1-5: 1-5: 1: 0.1-0.2; And
c) adding to the mixture a salt, a wetting agent and a sweetening agent to dissolve, adding a thickener, an abrasive, and a foaming agent to the mixture, and finally adding a flavoring agent.

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