KR100957051B1 - Marker genes based on nitrofurantoin treatment for screening of drug inducing pulmonary toxicity and screening method using thereof - Google Patents

Marker genes based on nitrofurantoin treatment for screening of drug inducing pulmonary toxicity and screening method using thereof Download PDF

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KR100957051B1
KR100957051B1 KR1020070139711A KR20070139711A KR100957051B1 KR 100957051 B1 KR100957051 B1 KR 100957051B1 KR 1020070139711 A KR1020070139711 A KR 1020070139711A KR 20070139711 A KR20070139711 A KR 20070139711A KR 100957051 B1 KR100957051 B1 KR 100957051B1
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류재천
김연정
송미
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한국과학기술연구원
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Abstract

본 발명은 폐독성 유발 약물 검색용 마커유전자 및 이를 이용한 검색 방법에 관한 것으로, 더욱 상세하게는 폐독성 유발 약물인 니트로푸란토인(nitrofurantoin)에 의해 유전자 발현이 증가 또는 감소하는 마커유전자 및 이를 이용한 폐독성 유발 약물의 검색 방법에 관한 것이다. 본 발명의 마커유전자는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 마커유전자로 이용하여 폐독성의 위험성을 지닌 약물 또는 화학물질을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 니트로푸란토인이 폐독성 및 부작용을 일으키는 작용 기작을 규명하는 도구로 이용될 수 있다.The present invention relates to a marker gene for pulmonary toxicity-inducing drug search and a search method using the same. The present invention relates to a method of searching for a toxic drug. The marker gene of the present invention can be usefully used for monitoring and determining drugs or chemicals having a risk of pulmonary toxicity by using reaction genes selected through DNA microarray chips as marker genes. It can be used as a tool to identify mechanisms of action that cause side effects.

니트로푸란토인, 마커유전자, 마이크로어레이, 폐독성 Nitrofurantoin, Marker Gene, Microarray, Lung Toxicity

Description

니트로푸란토인 처리에 따른, 폐독성 유발 약물 검색용 마커유전자 및 이를 이용한 검색 방법{Marker genes based on nitrofurantoin treatment for screening of drug inducing pulmonary toxicity and screening method using thereof}Marker genes based on nitrofurantoin treatment for screening of drug inducing pulmonary toxicity and screening method using according to nitrofurantoin treatment

본 발명은 폐독성 유발 약물 검색용 마커유전자 및 이를 이용한 검색 방법에 관한 것으로, 더욱 상세하게는 폐독성 유발 약물인 니트로푸란토인에 의해 유전자 발현이 증가 또는 감소하는 마커유전자 및 이를 이용한 폐독성 유발 약물의 검색 방법에 관한 것이다. The present invention relates to a marker gene for pulmonary toxicity-inducing drug search and a search method using the same. It is about the search method.

니트로푸란토인(nitrofurantoin)은 5-니트로푸란(nitrofuran)의 유도체로서 항균제제이며 요로감염증 치료제로서 사용된다. 니트로푸란토인 처방으로 약 85%의 환자에게서 폐 부작용이 발견되었으며, 이 중 특히 요로감염증 발생률이 많은 여성들에게서 많은 부작용이 나타나는 것으로 보고되었다(Bobbak V. et al., The Internet Journal of Pulmonary Medicine , 6(2), 2006). 니트로푸란토인 처방으로 발열, 반점상 구진, 관절통, 피로 등이 급성반응으로 나타나며, 일반적으로는 마른 기침, 가슴 통증 등이 나타나는 것으로 알려져 있다. 또한 호산구증다증(eosinopholia)도 나타나는 것으로 보고되었다. 그러나 이러한 반응들은 니트로푸란토인의 처방되는 용량과 연관성은 없다. 1개월 ~ 6개월 처방을 받는 경우, 마른기침, 호흡곤란, 미열, 청색증 등의 아급성 반응이 나타나며, 임상시험에서 적혈구 침강속도와 IgG가 증가되는 것으로 보고되었다(Sovojiarvi, A.R. et al., Scan . J. Respir . Dis ., 58:41-45, 1977). 6개월 ~ 6년 이상 니트로푸란토인을 처방받을 때에는 호흡곤란, 마른기침 등이 만성적인 증상으로 나타나며 심하면 폐섬유증이 유발되는 것으로 보고되었다(Mendex, J.L., Mayo Clin Proc ., 80:1298-1302, 2005). 만성질환에서 더 심해질 경우 객혈과 호흡부전이 나타타는 것으로 보고되었다. Nitrofurantoin is a derivative of 5-nitrofuran and is used as an antimicrobial agent and as a therapeutic agent for urinary tract infections. Pulmonary side effects were found in about 85% of patients with nitrofurantoin, especially among women with high incidence of urinary tract infections (Bobbak V. et al., The Internet Journal of Pulmonary Medicine , 6 (2), 2006). It is known that fever, spotty papules, joint pain, and fatigue are acute reactions with a prescription of nitrofurantoin. In general, a dry cough and chest pain are known. Eosinopholia has also been reported. However, these reactions are not associated with the prescribed dose of nitrofurantoin. When treated for 1 to 6 months, subacute reactions such as dry cough, dyspnea, mild fever, and cyanosis are reported, and clinical trials have reported increased erythrocyte sedimentation rate and IgG (Sovojiarvi, AR et al., Scan . J. Respir Dis, 58:. . 41-45, 1977). When prescribed nitrofurantoin for 6 months to 6 years or more, dyspnea, dry cough, etc. are chronic symptoms and severe pulmonary fibrosis has been reported (Mendex, JL, Mayo). Clin Proc . , 80: 1298-1302, 2005). It has been reported that hemoptysis and respiratory failure appear more severe in chronic diseases.

니트로푸란토인은 호기조건과 NADPH 존재 하에서 니트로푸란토인의 니트로기(nitro group)의 한 전자가 환원되면서 니트로 자유라디칼(R-NO2-)을 형성하고 이것은 산소와 반응하여 O2-, H2O2, OH- 등의 활성산소종을 생성하면서 폐독성을 나타내는 것으로 보고된바있다(Theodore, E.G., The American society for Pharmacology and Experimental Therapeutics, 49(4):308, 1997). 활성산소종은 PDGF(platelet-derived growth factor)에 의한 영향으로 JAK-STAT(Janus kinase-signal transducer and activater of transcription) 경로를 활성화시켜 폐섬유증을 유발하는 것으로 알려져 있다(Amy, R.S., Am J Physiol Cell Physiol ., 275:1640-1652, 1998). Nitrofurantoin forms a nitro free radical (R-NO 2- ) as one electron of the nitro group of nitrofurantoin is reduced under aerobic conditions and in the presence of NADPH, which reacts with oxygen to form O 2- , H 2. It has been reported to produce lung toxicity while producing reactive oxygen species such as O 2 and OH- (Theodore, EG, The American society for Pharmacology and Experimentalal Therapeutics , 49 (4): 308, 1997). Reactive oxygen species are known to cause pulmonary fibrosis by activating the JAK-STAT (Janus kinase-signal transducer and activater of transcription) pathway under the influence of platelet-derived growth factor (PDGF) (Amy, RS, Am J Physiol Cell Physiol ., 275: 1640-1652, 1998).

폐섬유증과 관련된 유전자로 MMP(matrix metalloprotease) 7, CCL7 이 보고된바 있으며, 동물 모델에서 FIZZ1도 이와 관련된 것으로 알려져 있다(Kaminski, N. et al., Proc Am Thorac Soc ., 3(4):339-44, 2006). 또한 앤지오텐신(angiotensin) Ⅱ(ANGⅡ)와 변형성 성장 인자(transforming growth factor, TGF)-β1도 폐섬유증과 관련된 것으로 보고되었다. TGF-β1이 앤지오텐신(angiotensin) Ⅱ 유형 1 수용체에 작용하여 Smad, p38 MAPK, JNK, PI3K(phosphatidylinositol 3-kinsae) 시그날 경로(signaling pathway)와 관련하여 폐섬유증을 유발하는 것으로 알려졌다(Martin, M.M., et al., Am J Physiol Cell Physiol ., 293(3):790-799, 2007).MMP (matrix metalloprotease) 7, CCL7 has been reported as a gene associated with pulmonary fibrosis, and FIZZ1 is also known to be associated with animal models (Kaminski, N. et al., Proc) . Am Thorac Soc . , 3 (4): 339-44, 2006). Angiotensin II (ANGII) and transforming growth factor (TGF) -β1 have also been reported to be associated with pulmonary fibrosis. TGF-β1 acts on angiotensin II type 1 receptors and is known to induce pulmonary fibrosis associated with signaling pathways of Smad, p38 MAPK, JNK, and phosphatidylinositol 3-kinsae (PI3K) (Martin, MM, et al., Am J Physiol Cell Physiol ., 293 (3): 790-799, 2007).

포유류 6종, 미생물 292종 등 여러 종의 게놈(genome) 염기서열 프로젝트가 완성되어 NCBI(National Center for Biotechnology Information)에 보고되었다. 이렇게 얻어진 막대한 양의 데이터를 기본으로 유전자의 기능을 연구하기 위하여 게놈-와이드 익스프레션(genome-wide expression) 연구가 이루어지고 있다. 한번의 실험으로 수천 개의 유전자의 발현을 분석하기 위하여 DNA 마이크로어레이(microarray) 분석을 수행한다(Schena, M., et al., Proc . Natl . Acad . Sci . USA 93: 10614-10619, 1996). Several genome sequencing projects, including six mammals and 292 microbes, have been completed and reported to the National Center for Biotechnology Information (NCBI). Genome-wide expression studies are being conducted to study the function of genes based on the vast amount of data obtained. DNA microarray analysis is performed to analyze the expression of thousands of genes in one experiment (Schena, M., et al., Proc . Natl . Acad . Sci . USA) . 93: 10614-10619, 1996).

마이크로어레이는 cDNA(complementary DNA)나 20-25 염기쌍(base pair) 길이의 올리고뉴클레오티드(oligonucleotide)들의 세트를 유리에 집적화한 것이다. cDNA 마이크로어레이는 학교 내의 연구실 또는 에질런트(Agilent), 제노믹 솔루션(Genomic Solutions) 등의 회사에서 칩 위에 cDNA 수집물을 기계적로 고정화 하거나 잉크젯(ink jetting) 방법을 이용하여 생산하고 있다(Sellheyer, K and Belbin, T.J., J. Am . Acad . Dermatol. 51: 681-692, 2004). 올리고뉴클레오티드 마이크로어레이는 애피메트릭스(Affymetrix)사에서 사진 식각 공정(photolithography)을 이용하여 칩 위에서 직접 합성 방법에 의해 만들고 있으며, 에질런트(Agillent)사 등에는 합성된 올리고뉴클레오티드를 고정화하는 방법으로 생산하고 있다(Sellheyer, K. et. al., Am . Acad . Dermatol. 51: 681-692, 2004).Microarrays integrate a set of oligonucleotides of complementary DNA (cDNA) or 20-25 base pairs in length on glass. cDNA microarrays are produced by labs in schools or by companies such as Agilent and Genomic Solutions, by mechanically immobilizing cDNA collections on chips or by using ink jetting (Sellheyer, K and Belbin, TJ, J. Am . Acad . Dermatol . 51: 681-692, 2004). Oligonucleotide microarrays are made by Affymetrix using photolithography directly on the chip. (Sellheyer, K. et. Al., Am . Acad . Dermatol . 51: 681-692, 2004).

유전자의 발현 분석을 위해서는 조직 등 시료에서 RNA를 얻어 DNA 마이크로어레이에 있는 올리고뉴클레오티드와 교잡반응을 수행한다. 얻어진 RNA는 형광이나 동위원소로 표지화하며, cDNA로 전환시킨다. 올리고 마이크로어레이는 주로 두개의 다른 형광(예: Cye3과 Cye5)으로 대조군과 실험군을 각각 표지화하여 같은 칩 상에서 동시에 교잡 반응을 수행한 후 광학적으로 이미지를 스캔하여 형광의 세기를 얻고 그 결과를 분석한다. 두 개의 형광 세기의 비율에 따라 유전자의 발현 여부를 결정한다(Somasundaram, K., et al., Genomics Proteomics I: 1-10, 2002).For gene expression analysis, RNA is obtained from samples such as tissues and hybridized with oligonucleotides in a DNA microarray. The obtained RNA is labeled with fluorescence or isotope and converted to cDNA. Oligo microarrays are mainly labeled with two different fluorescences (e.g., Cye3 and Cye5) to perform hybridization reactions on the same chip at the same time by labeling the control and experimental groups, respectively, and then optically scanning the images to obtain fluorescence intensity and analyzing the results. . Gene expression is determined according to the ratio of two fluorescence intensities (Somasundaram, K., et al. , Genomics Proteomics I: 1-10, 2002).

최근 DNA 마이크로어레이 기술을 이용한 첨단 기법인 독성 유전체학(Toxicogenomics) 연구 등과 접목하여 대량(high throughput)으로 의약품 및 신의약 후보물질은 물론 모든 화학물질에 의한 특정 조직이나 세포주에서 발현되는 유전자들의 발현 패턴의 분석, 양적 분석이 가능해졌다. 이에 따라 특정 세포 내 에서 특정 유전자의 발현 빈도를 분석함으로써 약물의 부작용과 관련된 유전자의 발굴이 가능하며, 이를 통하여 약물의 작용 및 부작용에 따른 분자적 메커니즘을 이해하게 될 것이며 나아가 독성 및 부작용을 유발하는 물질의 검색 및 진단이 가능하게 될 것이다. In combination with recent research on toxic genomics (Toxicogenomics), which is an advanced technique using DNA microarray technology, the expression pattern of genes expressed in specific tissues or cell lines by all chemicals as well as drug and new drug candidates in high throughput. Analysis and quantitative analysis are now possible. Accordingly, by analyzing the frequency of expression of specific genes in specific cells, it is possible to discover genes related to side effects of drugs, through which the molecular mechanisms of the actions and side effects of drugs are understood, and further, toxicity and side effects are induced. Search and diagnosis of the material will be possible.

이에 본 발명자들은 인간 유전자 4만 4천 개가 집적된 올리고 마이크로어레이를 이용하여 니트로푸란토인의 유전자 발현 프로파일을 인간 기관지 상피 세포인 BEAS-2B 세포주에서 관찰 및 분석함으로써 니트로푸란토인에 의해 과발현 또는 저발현 되는 유전자를 발굴하고, 실시간(real-time) 정량 RT-PCR 방법에 의해 상기 유전자들의 발현 양상을 확인하여 폐독성 유발 약물을 검출할 수 있는 마커유전자 및 이를 이용한 폐독성 유발 약물의 검색 방법을 확립함으로써 본 발명을 완성하였다.Accordingly, the present inventors observed and analyzed the gene expression profile of nitrofurantoin in a BEAS-2B cell line, which is a human bronchial epithelial cell, using an oligo microarray in which 44,000 human genes were accumulated. Establish a marker gene that can detect pulmonary toxicity-inducing drugs and identify a method for searching for pulmonary toxicity-inducing drugs by identifying the genes to be identified and confirming the expression patterns of the genes by real-time quantitative RT-PCR method By this, the present invention was completed.

본 발명의 목적은 폐독성 유발 약물인 니트로푸란토인에 의해 과발현 또는 저발현되는 마커유전자 및 상기 마커유전자를 이용한 폐독성 유발 약물 검색 방법을 제공하는 것이다.An object of the present invention is to provide a marker gene overexpressed or underexpressed by nitrofurantoin, a pulmonary toxicity-inducing drug, and a pulmonary toxicity-inducing drug search method using the marker gene.

상기 목적을 달성하기 위하여, 본 발명은 폐독성 유발 약물인 니트로푸란토인에 의해 기관지 상피 세포에서 발현변화를 일으키는 것을 특징으로 하는 폐독성 유발 약물 검색용 마커유전자를 제공한다.In order to achieve the above object, the present invention provides a marker gene for pulmonary toxicity-induced drug search, characterized in that the expression changes in bronchial epithelial cells by the pulmonary toxicity-inducing drug nitrofurantoin.

또한, 본 발명은 상기 마커유전자 서열의 전부 또는 일부를 포함하는 올리고 뉴클레오티드, 또는 이의 상보가닥 분자가 집적된 폐독성 유발 약물 검색용 DNA 마이크로어레이 칩을 제공한다.In addition, the present invention provides a DNA microarray chip for pulmonary toxicity-induced drug search in which an oligonucleotide, or a complementary strand molecule thereof, containing all or part of the marker gene sequence is integrated.

또한, 본 발명은 상기 마커유전자를 이용한 폐독성 유발 약물 검색 방법을 제공한다.The present invention also provides a method for screening for pulmonary toxicity-inducing drugs using the marker gene.

아울러, 본 발명은 상기 DNA 마이크로어레이 칩을 포함하는 폐독성 유발 약물 검색용 키트를 제공한다.In addition, the present invention provides a kit for pulmonary toxicity-induced drug search comprising the DNA microarray chip.

본 발명의 폐독성 유발 약물 검색용 마커유전자 및 이를 이용한 폐독성 유발 약물의 검색 방법은 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 마커유전자로 이용하여 새로운 폐독성의 위험성을 지닌 약물 또는 화학물질을 모니터링하거나 위해성을 판정하는데 유용하며, 니트로푸란토인에 의해 야기되는 폐독성 및 부작용을 일으키는 작용 기작을 규명하는 도구로 이용될 수 있다.The marker gene for searching for pulmonary toxicity-inducing drug of the present invention and a method for searching for pulmonary toxicity-inducing drug using the same are selected from the DNA microarray chip as a marker gene. It is useful for monitoring or determining risk and can be used as a tool to identify mechanisms of action that cause pulmonary toxicity and side effects caused by nitrofurantoin.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 폐독성 유발 약물 검색용 마커유전자를 제공한다.The present invention provides a marker gene for pulmonary toxicity induced drug search.

상기 마커 유전자는 니트로푸란토인에 의해 자극받은 인간 기관지 상피 세포에서 발현 변화를 일으키는 것을 특징으로 한다.The marker gene is characterized by causing a change in expression in human bronchial epithelial cells stimulated by nitrofurantoin.

상기 마커유전자는 세포자살(apoptosis), 전사(transcription), 면역반응(immune response), 이온수송(ion transport), 세포주기(cell cycle), 단백질 분해(proteolysis), DNA 대사(DNA metabolism), 해당과정(glycolysis), 혈관신생(angiogenesis), TGF-β, MAPK 또는 JAK-STAT에 관련된 유전자로 구성되어 있다.The marker genes include apoptosis, transcription, immune response, ion transport, cell cycle, proteolysis, DNA metabolism, and the like. It consists of genes involved in glycolysis, angiogenesis, TGF-β, MAPK or JAK-STAT.

본 발명은 하기와 같이 구성된 군에서 선택되는 것을 특징으로 하는 마커유전자를 제공한다: The present invention provides a marker gene, characterized in that selected from the group consisting of:

유전자등록번호(Genebank) BE378852(Transcribed locus), 유전자등록번호(Genebank) BG527520[Transcribed locus, moderately similar to XP_540962.1 PREDICTED: similar to centrin 4(Canis familiaris)]), 유전자등록번호(Genebank) NM_004797(Adiponectin, C1Q and collagen domain containing), 유전자등록번호(Genebank) AF153821[Alcohol dehydrogenase IB(class I), beta polypeptide], 유전자등록번호(Genebank) NM_006818(Myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila); translocated to, 11), 유전자등록번호(Genebank)BP871540(Transcribed locus), 유전자등록번호(Genebank) AF269088(Ankyrin repeat domain 30B), 유전자등록번호(Genebank) BC018064(Activating transcription factor 6), 유전자등록번호(Genebank) XM_208270(Acrosin-like protease), 유전자등록번호(Genebank) NM_004323(BCL2-associated athanogene), 유전자등록번호(Genebank) BE816155(RC0-BN0198-220500-021-f04 BN0198 Homo sapiens cDNA, mRNA sequence.), 유전자등록번호(Genebank) NM_003670(Basic helix-loop-helix domain containing, class B, 2), 유전자등록번호(Genebank) BE614991[Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein(Pan troglodytes)], 유전자등록번호(Genebank) CN484301[Transcribed locus, moderately similar to XP_227769.2 PREDICTED: similar to Ac1147(Rattus norvegicus)], 유전자등록번호(Genebank)AK027152(Chromosome 11 open reading frame 1), 유전자등록번호(Genebank) XM_290629(Chromosome 14 open reading frame 78), 유전자등록번호(Genebank) NM_173817(Chromosome 18 open reading frame 58), 유전자등록번호(Genebank) CR625148(Hypothetical LOC541469 protein), 유전자등록번 호(Genebank)BX537504(Complement component 2), 유전자등록번호(Genebank) BX281604(Chromosome 20 open reading frame 187), 유전자등록번호(Genebank) BU618438(Chromosome 20 open reading frame 77), 유전자등록번호(Genebank) BC020888(Metallophosphoesterase domain containing 1), 유전자등록번호(Genebank)NM_213720(Chromosome 22 open reading frame 16), 유전자등록번호(Genebank) BC027919(Chromosome 5 open reading frame 16), 유전자등록번호(Genebank) NM_030568(Chromosome 6 open reading frame 147), 유전자등록번호(Genebank) AK021476(Chromosome 6 open reading frame 164), 유전자등록번호(Genebank) BC036421(Chromosome 9 open reading frame 103), 유전자등록번호(Genebank) NM_153707(Chromosome 9 open reading frame 138), 유전자등록번호(Genebank) NM_203403(Chromosome 9 open reading frame 150), 유전자등록번호(Genebank) NM_021208(Chromosome 9 open reading frame 27), 유전자등록번호(Genebank)NM_053056(Cyclin D1), 유전자등록번호(Genebank) NM_001295[Chemokine(C-C motif) receptor 1], 유전자등록번호(Genebank) AK126349(CD200 receptor 1), 유전자등록번호(Genebank) AK026467(CD22 antigen), 유전자등록번호(Genebank) AK054921(Cerebellar degeneration-related protein 1, 34kDa), 유전자등록번호(Genebank) NM_012125(Cholinergic receptor, muscarinic 5), 유전자등록번호(Genebank) NM_003992(CDC-like kinase 3), 유전자등록번호(Genebank) AK022155(Coproporphyrinogen oxidase), 유전자등록번호(Genebank) AK090482[Chemokine(C-X-C motif) ligand 12(stromal cell-derived factor 1)], 유 전자등록번호(Genebank) NM_152693(Chromosome X open reading frame 50), 유전자등록번호(Genebank) BX108307(Defensin, beta 126), 유전자등록번호(Genebank) AB051529[Dispatched homolog 2(Drosophila)], 유전자등록번호(Genebank) BC014951(chromosome 20 open reading frame 107), 유전자등록번호(Genebank) AK095541[DnaJ(Hsp40) homolog, subfamily A, member 1], 유전자등록번호(Genebank) BC036806(Extracellular matrix protein 2, female organ and adipocyte specific), 유전자등록번호(Genebank) BI830993(Eukaryotic translation initiation factor 4E), 유전자등록번호(Genebank) BX537939[Elastin(supravalvular aortic stenosis, Williams-Beuren syndrome)], 유전자등록번호(Genebank) AB051895(Epiplakin 1), 유전자등록번호(Genebank) AB209361[Fas(TNF receptor superfamily, member 6)], 유전자등록번호(Genebank) NM_033480(F-box protein 9), 유전자등록번호(Genebank)BC034763(Ferredoxin reductase), 유전자등록번호(Genebank) NM_005141(Fibrinogen beta chain), 유전자등록번호(Genebank) AL832445(Hypothetical protein FLJ25770), 유전자등록번호(Genebank) XM_499008(Hypothetical gene supported by AK124699), 유전자등록번호(Genebank) AK124499(RGS9 anchor protein), 유전자등록번호(Genebank) CR936597(Fibrous sheath interacting protein 2), 유전자등록번호(Genebank) NM_004293(Guanine deaminase), 유전자등록번호(Genebank) NM_177483(Glycosylphosphatidylinositol specific phospholipase D1), 유전자등록번호(Genebank) AK074729(G protein-coupled receptor 19), 유전자등록번 호(Genebank) AK122626(G protein-coupled receptor 82), 유전자등록번호(Genebank) NM_014619(Glutamate receptor, ionotropic, kainate 4), 유전자등록번호(Genebank) NM_002133[Heme oxygenase(decycling) 1], 유전자등록번호(Genebank) CB124304[Transcribed locus, strongly similar to NP_001604.1 alpha 2 actin; alpha-cardiac actin(Homo sapiens)], 유전자등록번호(Genebank) BE745738(CDNA clone IMAGE:3928921), 유전자등록번호(Genebank) BC043212(Hypothetical LOC401057), 유전자등록번호(Genebank) BC008624(Homo sapiens cDNA clone MGC:18299 IMAGE:4179890, complete cds.), 유전자등록번호(Genebank) AK024336(Homo sapiens cDNA FLJ14274 fis, clone PLACE1004979.), 유전자등록번호(Genebank) AK057952(CDNA FLJ25223 fis, clone STM00880), 유전자등록번호(Genebank) AK123483(CDNA FLJ41489 fis, clone BRTHA2004582), 유전자등록번호(Genebank) BU570278[Similar to dJ1153D9.4(novel protein)], 유전자등록번호(Genebank) BE614991[Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein(Pan troglodytes)], 유전자등록번호(Genebank) T77197(TBC1 domain family, member 3B), 유전자등록번호(Genebank) AF119839(predicted protein of HQ0889; Homo sapiens PRO0889 mRNA, complete cds.), 유전자등록번호(Genebank)AF119912(predicted protein of HQ3073; Homo sapiens PRO3073 mRNA, complete cds.), 유전자등록번호(Genebank) BM467031(Ribosomal protein S2), 유전자등록번호(Genebank) CA943742[Transcribed locus, strongly similar to XP_429266.1 PREDICTED: hypothetical protein XP_429266(Gallus gallus)], 유전자등록번호(Genebank) AV727525(Transcribed locus), 유전자등록번호(Genebank) BC015351(Homo sapiens, clone IMAGE:4420333, mRNA), 유전자등록번호(Genebank) BG759321(Homo sapiens, clone IMAGE:4852110, mRNA), 유전자등록번호(Genebank) BF337308(Homeodomain-only protein), 유전자등록번호(Genebank) AK223249(Homeo box B7), 유전자등록번호(Genebank) NM_021192(Homeo box D11), 유전자등록번호(Genebank) NM_005345(Heat shock 70kDa protein 1A), 유전자등록번호(Genebank)NM_006597(Heat shock 70kDa protein 8), 유전자등록번호(Genebank) U88896, 유전자등록번호(Genebank) NM_134470(Interleukin 1 receptor accessory protein), 유전자등록번호(Genebank) BC069137[Interleukin 5(colony-stimulating factor, eosinophil)], 유전자등록번호(Genebank) AB039920(KCNQ1 downstream neighbor), 유전자등록번호(Genebank) AB033055(Outer dense fiber of sperm tails 2-like), 유전자등록번호(Genebank) AK001632(Hypothetical protein FLJ10770), 유전자등록번호(Genebank) BC042437[Keratin 14(epidermolysis bullosa simplex, Dowling-Meara, Koebner)], 유전자등록번호(Genebank) BX647923(Keratin 17), 유전자등록번호(Genebank) BC025396[Spermatogenesis associated 18 homolog(rat)], 유전자등록번호(Genebank) BC016613(Similar to hypothetical protein FLJ13841), 유전자등록번호(Genebank) BU536672(Ribosomal protein L22-like 1), 유전자등록번호(Genebank) BC012203(Hypothetical protein LOC284361), 유전자등록번호(Genebank) BC044311(Hypothetical protein LOC339344), 유전자등록번호(Genebank) AK022806(Hypothetical protein LOC340281), 유전자등록번호(Genebank) XM_371643(Similar to hypothetical protein MGC39725), 유전자등록번호(Genebank) XM_372625(similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author), 유전자등록번호(Genebank) XM_498623(LOC440309), 유전자등록번호(Genebank) XM_496818(similar to RIKEN cDNA 2410146L05), 유전자등록번호(Genebank) XM_497458(Similar to large subunit ribosomal protein L36a), 유전자등록번호(Genebank) AK127395(Ankyrin repeat domain 37), 유전자등록번호(Genebank) AK126961(Leucine rich repeat transmembrane neuronal 4), 유전자등록번호(Genebank) AK026984(Multiple C2 domains, transmembrane 2), 유전자등록번호(Genebank) BE644770(Mitofusin 1), 유전자등록번호(Genebank) AK123160(Chromosome 1 open reading frame 162), 유전자등록번호(Genebank) AK125877(Hypothetical protein MGC27016), 유전자등록번호(Genebank) AK124107(Leucine rich repeat containing 43), 유전자등록번호(Genebank) AK097334(Hypothetical protein MGC45840), 유전자등록번호(Genebank) AK123482(Microsomal glutathione S-transferase 1), 유전자등록번호(Genebank) AK128769(calcium channel, voltage-dependent, beta 2 subunit), 유전자등록번호(Genebank) AY154467(NACHT, leucine rich repeat and PYD containing 12), 유전자등록번호(Genebank) AK124709(N-myc downstream regulated gene 1), 유전자등록번호(Genebank) NM_016931(NADPH oxidase 4), 유전자등록번호(Genebank) BX640730(Nebulin-related anchoring protein), 유전자등록번호(Genebank) AA524504(Full length insert cDNA clone ZE12C10), 유전자등록번호(Genebank) NM_002563(Purinergic receptor P2Y, G-protein coupled, 1), 유전자등록번호(Genebank) AL834134(Protocadherin 15), 유전자등록번호(Genebank) NM_001011515(PDZ and LIM domain 5), 유전자등록번호(Genebank) S81916(Phosphoglycerate kinase 1), 유전자등록번호(Genebank) NM_003662[Pirin(iron-binding nuclear protein)], 유전자등록번호(Genebank) NM_033256[Protein phosphatase 1, regulatory(inhibitor) subunit 14A], 유전자등록번호(Genebank) NM_014068(Psoriasis susceptibility 1 candidate 1), 유전자등록번호(Genebank) NM_002900(Retinol binding protein 3, interstitial), 유전자등록번호(Genebank) AK055999(Ret finger protein-like 2), 유전자등록번호(Genebank)AB209019(Regulator of G-protein signalling 4), 유전자등록번호(Genebank) NM_004040(Ras homolog gene family, member B), 유전자등록번호(Genebank)BU902070(Ribosomal protein L36a-like), 유전자등록번호(Genebank) BF983391(Ribosomal protein S27-like), 유전자등록번호(Genebank)NM_173640[R-spondin homolog(Xenopus laevis)], 유전자등록번호(Genebank) BG182941[Transcribed locus, weakly similar to XP_510104.1 PREDICTED: similar to hypothetical protein FLJ25224(Pan troglodytes)], 유전자등록번호(Genebank) NM_004057(S100 calcium binding protein G), 유전자등록번호(Genebank) BC041022(SCAN domain containing 1), 유전자등록번호(Genebank)NM_002639[Serpin peptidase inhibitor, clade B(ovalbumin), member 5], 유전자등록번 호(Genebank)NM_005668(ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4), 유전자등록번호(Genebank) NM_003830(Sialic acid binding Ig-like lectin 5), 유전자등록번호(Genebank) BC053934[Sirtuin(silent mating type information regulation 2 homolog) 4(S. cerevisiae)], 유전자등록번호(Genebank) NM_052832(Solute carrier family 26, member 7), 유전자등록번호(Genebank) NM_014332(Small muscle protein, X-linked), 유전자등록번호(Genebank) BC026316[Suppression of tumorigenicity 18(breast carcinoma)(zinc finger protein)], 유전자등록번호(Genebank) AK122707(Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3), 유전자등록번호(Genebank) AK026585(Tumor-associated calcium signal transducer 1), 유전자등록번호(Genebank) AL038125(Zinc finger, AN1-type domain 3), 유전자등록번호(Genebank) NM_000361(Thrombomodulin), 유전자등록번호(Genebank) BC010553(TIMP metallopeptidase inhibitor 4), 유전자등록번호(Genebank) NM_003840(Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), 유전자등록번호(Genebank) NM_004881(Tumor protein p53 inducible protein 3), 유전자등록번호(Genebank) NM_033229(Tripartite motif-containing 15), 유전자등록번호(Genebank) BC063872(Tripartite motif-containing 9), 유전자등록번호(Genebank) NM_003314(Tetratricopeptide repeat domain 1), 유전자등록번호(Genebank) AK024002(Tubulin, alpha 4), 유전자등록번호(Genebank) NM_006087(Tubulin, beta 4), 유전자등록번호(Genebank) AF219946(Tubby like protein 4), 유전자등록번호(Genebank) AK025742[Uncoupling protein 2(mitochondrial, proton carrier)], 유전자등록번호(Genebank) AI699331(Transcribed locus), 유전자등록번호(Genebank) AF055634[Unc-5 homolog C(C. elegans)], 유전자등록번호(Genebank) AK023940(Homo sapiens cDNA FLJ13878 fis, clone THYRO1001411), 유전자등록번호(Genebank) BE815283(Transcribed locus), 유전자등록번호(Genebank) BM913228[Full-length cDNA clone CS0DI020YI20 of Placenta Cot 25-normalized of Homo sapiens(human)], 유전자등록번호(Genebank) BX099682(Transcribed locus), 유전자등록번호(Genebank) BX640772[MRNA; cDNA DKFZp686P02129(from clone DKFZp686P02129)], 유전자등록번호(Genebank) AK094191(Chromosome 8 open reading frame 34), 유전자등록번호(Genebank) NM_033400(Zinc finger homeobox 2), 유전자등록번호(Genebank) XM_040592(Zinc finger protein 469), 유전자등록번호(Genebank) NM_007313(V-abl Abelson murine leukemia viral oncogene homolog 1), 유전자등록번호(Genebank) NM_203380(Acyl-CoA synthetase long-chain family member 5), 유전자등록번호(Genebank) BC040531(Activin A receptor, type IB), 유전자등록번호(Genebank) Y13786[ADAM metallopeptidase domain 19(meltrin beta)], 유전자등록번호(Genebank) AJ250725(ADAM metallopeptidase with thrombospondin type 1 motif, 12), 유전자등록번호(Genebank) AJ315733(ADAM metallopeptidase with thrombospondin type 1 motif, 15), 유전자등록번호(Genebank) NM_133638(ADAM metallopeptidase with thrombospondin type 1 motif, 19), 유전자등록번 호(Genebank) NM_014243(ADAM metallopeptidase with thrombospondin type 1 motif, 3), 유전자등록번호(Genebank) NM_207517(ADAMTS-like 3), 유전자등록번호(Genebank) BC013780(Adenosine A2a receptor), 유전자등록번호(Genebank) AK160382(AT-hook transcription factor), 유전자등록번호(Genebank) AK122839(V-akt murine thymoma viral oncogene homolog 2), 유전자등록번호(Genebank) AK128709(Aldehyde dehydrogenase 1 family, member A2), 유전자등록번호(Genebank) NM_003748(Aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(Genebank) NM_172196(TFIIA-alpha/beta-like factor), 유전자등록번호(Genebank) NM_152526[Amyotrophic lateral sclerosis 2(juvenile) chromosome region, candidate 19], 유전자등록번호(Genebank) NM_139314(Angiopoietin-like 4), 유전자등록번호(Genebank) NM_000037(Ankyrin 1, erythrocytic), 유전자등록번호(Genebank) AJ583444[Ankyrin repeat domain 2(stretch responsive muscle)], 유전자등록번호(Genebank) NM_145641(Apolipoprotein L, 3), 유전자등록번호(Genebank) BC006276(Apolipoprotein L, 4), 유전자등록번호(Genebank) NM_003224(ADP-ribosylation factor related protein 1), 유전자등록번호(Genebank) NM_021069(Sorbin and SH3 domain containing 2), 유전자등록번호(Genebank) AK090447(Rho GTPase activating protein 19), 유전자등록번호(Genebank) NM_015071(Rho GTPase activating protein 26), 유전자등록번호(Genebank) NM_032199[AT rich interactive domain 5B(MRF1-like)], 유전자등록번호(Genebank) NM_183376(Arrestin domain containing 4), 유전자등록번 호(Genebank) AK000643(Ankyrin repeat and SOCS box-containing 9), 유전자등록번호(Genebank) AB209032(Activating transcription factor 3), 유전자등록번호(Genebank) NM_032827[Atonal homolog 8(Drosophila)], 유전자등록번호(Genebank) BX537444(ATPase, Ca++ transporting, plasma membrane 4), 유전자등록번호(Genebank) NM_006045(ATPase, Class II, type 9A), 유전자등록번호(Genebank) AB011106(Attractin-like 1), 유전자등록번호(Genebank) NM_000707(Arginine vasopressin receptor 1B), 유전자등록번호(Genebank)CR933657[Axin 2(conductin, axil)], 유전자등록번호(Genebank) NM_152384(Bardet-Biedl syndrome 5), 유전자등록번호(Genebank) NM_021948(Brevican), 유전자등록번호(Genebank) AA608624(Breast carcinoma amplified sequence 3), 유전자등록번호(Genebank) NM_017745(BCL6 co-repressor), 유전자등록번호(Genebank) S72478, 유전자등록번호(Genebank) NM_001710(B-factor, properdin), 유전자등록번호(Genebank) CR749373(chromosome 1 open reading frame 178), 유전자등록번호(Genebank) AL713697(Bridging integrator 1), 유전자등록번호(Genebank) NM_001165(Baculoviral IAP repeat-containing 3), 유전자등록번호(Genebank) BM984396(Plasminogen-like B2), 유전자등록번호(Genebank) NM_001203(Bone morphogenetic protein receptor, type IB), 유전자등록번호(Genebank) AK098095(V-raf murine sarcoma viral oncogene homolog B1), 유전자등록번호(Genebank) NM_014299(Bromodomain containing 4), 유전자등록번호(Genebank) BC016743[BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB(S. cerevisiae)], 유전자등록번호(Genebank)NM_001728[Basigin(OK blood group)], 유전자등록번호(Genebank) NM_052876[BTB(POZ) domain containing 14B], 유전자등록번호(Genebank) BX537534[BTB(POZ) domain containing 9], 유전자등록번호(Genebank) BX107768(Transcribed locus), 유전자등록번호(Genebank) AY665468[Ras association(RalGDS/AF-6) domain family 8], 유전자등록번호(Genebank) NM_033513(Chromosome 19 open reading frame 20), 유전자등록번호(Genebank) BC068439(Chromosome 19 open reading frame 28), 유전자등록번호(Genebank) AY358424(C1q and tumor necrosis factor related protein 1), 유전자등록번호(Genebank) NM_001733(Complement component 1, r subcomponent), 유전자등록번호(Genebank) NM_080834(Chromosome 20 open reading frame 152), 유전자등록번호(Genebank) AK000652(Chromosome 20 open reading frame 57), 유전자등록번호(Genebank) BC063852(Complement component 3), 유전자등록번호(Genebank) AF251038(Chromosome 5 open reading frame 5), 유전자등록번호(Genebank) BX640641(Chromosome 6 open reading frame 107), 유전자등록번호(Genebank) AL832416(Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1), 유전자등록번호(Genebank) BC021552(Calcium channel, voltage-dependent, gamma subunit 6), 유전자등록번호(Genebank) AL117636(Calneuron 1), 유전자등록번호(Genebank) AJ519841(Calmodulin regulated spectrin-associated protein 1), 유전자등록번호(Genebank) AK128124(Calpain 5), 유전자등록번 호(Genebank) NM_002982[Chemokine(C-C motif) ligand 2], 유전자등록번호(Genebank) BC020698[Chemokine(C-C motif) ligand 20], 유전자등록번호(Genebank) BC071718[Chemokine(C-C motif) ligand 4-like 1], 유전자등록번호(Genebank) CR614015(CD14 antigen), 유전자등록번호(Genebank) NM_001004196(CD200 antigen), 유전자등록번호(Genebank) AK090470[CD33 antigen(gp67)], 유전자등록번호(Genebank) NM_001773(CD34 antigen), 유전자등록번호(Genebank) BC025729(CD99 antigen-like 2), 유전자등록번호(Genebank) AB051503(Centaurin, beta 5), 유전자등록번호(Genebank)NM_032221(Chromodomain helicase DNA binding protein 6), 유전자등록번호(Genebank) NM_000740(Cholinergic receptor, muscarinic 3), 유전자등록번호(Genebank) NM_016369(Claudin 18), 유전자등록번호(Genebank) AB007969[Calmin(calponin-like, transmembrane)], 유전자등록번호(Genebank) AJ278018(Calsyntenin 2), 유전자등록번호(Genebank) AJ626900(Cardiomyopathy associated 1), 유전자등록번호(Genebank) NM_020872[Contactin 3(plasmacytoma associated)], 유전자등록번호(Genebank) NM_004370(Collagen, type XII, alpha 1), 유전자등록번호(Genebank) Z74615(Collagen, type I, alpha 1), 유전자등록번호(Genebank) NM_001845(Collagen, type IV, alpha 1), 유전자등록번호(Genebank) NM_033380[Collagen, type IV, alpha 5(Alport syndrome)], 유전자등록번호(Genebank) NM_000393(Collagen, type V, alpha 2), 유전자등록번호(Genebank) NM_001848(Collagen, type VI, alpha 1), 유전자등록번호(Genebank) NM_001850(Collagen, type VIII, alpha 1), 유전자등록번호(Genebank)AK124894(Corticotropin releasing hormone receptor 1), 유전자등록번호(Genebank) BC021117[Colony stimulating factor 1(macrophage)], 유전자등록번호(Genebank) BE873976[Colony stimulating factor 2(granulocyte-macrophage)], 유전자등록번호(Genebank) NM_172220[Colony stimulating factor 3(granulocyte)], 유전자등록번호(Genebank) AK092391(Cystatin E/M), 유전자등록번호(Genebank) NM_147780(Cathepsin B), 유전자등록번호(Genebank) NM_001334(Cathepsin O), 유전자등록번호(Genebank) NM_198700(CUG triplet repeat, RNA binding protein 1), 유전자등록번호(Genebank) NM_015267[Cut-like 2(Drosophila)], 유전자등록번호(Genebank) NM_001511[Chemokine(C-X-C motif) ligand 1(melanoma growth stimulating activity, alpha)], 유전자등록번호(Genebank)BC053653[Chemokine(C-X-C motif) ligand 2], 유전자등록번호(Genebank) NM_002090[Chemokine(C-X-C motif) ligand 3], 유전자등록번호(Genebank) NM_002994[Chemokine(C-X-C motif) ligand 5], 유전자등록번호(Genebank) U81234[Chemokine(C-X-C motif) ligand 6(granulocyte chemotactic protein 2)], 유전자등록번호(Genebank) NM_000497(Cytochrome P450, family 11, subfamily B, polypeptide 1), 유전자등록번호(Genebank) NM_004393[Dystroglycan 1(dystrophin-associated glycoprotein 1)], 유전자등록번호(Genebank) BX537651(Discoidin domain receptor family, member 2), 유전자등록번호(Genebank) AB209635(Diacylglycerol kinase, zeta 104kDa), 유전자등록번 호(Genebank) AB209653[Dehydrogenase/reductase(SDR family) member 2], 유전자등록번호(Genebank) NM_004753[Dehydrogenase/reductase(SDR family) member 3], 유전자등록번호(Genebank) AL832637(Taxilin alpha), 유전자등록번호(Genebank) BX640676(Regeneration associated muscle protease), 유전자등록번호(Genebank) XM_291222(Integrator complex subunit 1), 유전자등록번호(Genebank) NM_031294(Leucine rich repeat containing 48), 유전자등록번호(Genebank)NM_182643(Deleted in liver cancer 1), 유전자등록번호(Genebank) NM_001365[Discs, large homolog 4(Drosophila)], 유전자등록번호(Genebank) NM_007329(Deleted in malignant brain tumors 1), 유전자등록번호(Genebank) BM808864(Dopamine receptor D1 interacting protein), 유전자등록번호(Genebank) AK057845(Ephrin-A1), 유전자등록번호(Genebank) NM_012199(Eukaryotic translation initiation factor 2C, 1), 유전자등록번호(Genebank) BX537368[E74-like factor 3(ets domain transcription factor, epithelial-specific)], 유전자등록번호(Genebank) NM_001426(Engrailed homolog 1), 유전자등록번호(Genebank) BC054508(Erythrocyte membrane protein band 4.1 like 5), 유전자등록번호(Genebank) AF073727(Epsin 1), 유전자등록번호(Genebank) BC060883(Epsin 2), 유전자등록번호(Genebank) AL831953[Epithelial stromal interaction 1(breast)], 유전자등록번호(Genebank) D79987[Extra spindle poles like 1(S. cerevisiae)], 유전자등록번호(Genebank) NM_00198[Ets variant gene 6(TEL oncogene)], 유전자등록번호(Genebank) BX648769(Ewing sarcoma breakpoint region 1), 유전자등록번호(Genebank) NM_014661(Family with sequence similarity 53, member B), 유전자등록번호(Genebank) NM_001004019(Fibulin 2), 유전자등록번호(Genebank) AK125888(F-box protein 32), 유전자등록번호(Genebank) NM_002016(Filaggrin), 유전자등록번호(Genebank) NM_024669(FLJ11795 protein), 유전자등록번호(Genebank) NM_024817(Hypothetical protein FLJ13710), 유전자등록번호(Genebank) NM_017631(Hypothetical protein FLJ20035), 유전자등록번호(Genebank) BQ279256(Hypothetical protein FLJ20294), 유전자등록번호(Genebank) AK123641(Cleavage and polyadenylation specific factor 3-like), 유전자등록번호(Genebank) AK024488(Hypothetical protein FLJ21438), 유전자등록번호(Genebank) AL832659(Hypothetical protein FLJ22833), 유전자등록번호(Genebank) NM_181645(Hypothetical protein FLJ25393), 유전자등록번호(Genebank) AL833626(Zinc finger, DHHC-type containing 20), 유전자등록번호(Genebank) NM_173621(Chromosome 17 open reading frame 44), 유전자등록번호(Genebank) NM_001460(Flavin containing monooxygenase 2), 유전자등록번호(Genebank) NM_001461(Flavin containing monooxygenase 5), 유전자등록번호(Genebank) NM_212482(Fibronectin 1), 유전자등록번호(Genebank) AB033068[Fizzy/cell division cycle 20 related 1(Drosophila)], 유전자등록번호(Genebank) NM_080612(GRB2-associated binding protein 3), 유전자등록번호(Genebank) BC051463[Gamma-aminobutyric acid(GABA) B receptor, 1], 유전자등록번호(Genebank) NM_198321[UDP-N-acetyl-alpha-D-galactosamine:polypeptide N- acetylgalactosaminyltransferase 10(GalNAc-T10)], 유전자등록번호(Genebank) NM_198335(Glucosidase, alpha; neutral AB), 유전자등록번호(Genebank) NM_002048(Growth arrest-specific 1), 유전자등록번호(Genebank) AK127845(GATA binding protein 2), 유전자등록번호(Genebank) NM_000514(Glial cell derived neurotrophic factor), 유전자등록번호(Genebank) NM_000165[Gap junction protein, alpha 1, 43kDa(connexin 43)], 유전자등록번호(Genebank) BC043390(Glucagon-like peptide 2 receptor), 유전자등록번호(Genebank) AF288389(Glycosyltransferase 25 domain containing 2), 유전자등록번호(Genebank) BC051726[Glutamate-ammonia ligase(glutamine synthetase)], 유전자등록번호(Genebank) CR936770[Guanine nucleotide binding protein(G protein), alpha activating activity polypeptide O], 유전자등록번호(Genebank) BC095544(G protein-coupled receptor 32), 유전자등록번호(Genebank) NM_000841(Glutamate receptor, metabotropic 4), 유전자등록번호(Genebank) X99975(H.sapiens mRNA for hRTR/hGCNF protein.), 유전자등록번호(Genebank) CR624693(3-hydroxyanthranilate 3,4-dioxygenase), 유전자등록번호(Genebank) AF227948(Hepatitis B virus x associated protein), 유전자등록번호(Genebank) AL834223(Histone deacetylase 11), 유전자등록번호(Genebank) NM_021170[Hairy and enhancer of split 4(Drosophila)], 유전자등록번호(Genebank) AK075040[Hairy and enhancer of split 6(Drosophila)], 유전자등록번호(Genebank) BC101593(Histone 1, H1a), 유전자등록번호(Genebank) BU603483(Histone 1, H1e), 유전자등록번 호(Genebank)BC065757(CDNA clone IMAGE:4556546), 유전자등록번호(Genebank) AK091904(CDNA FLJ34585 fis, clone KIDNE2008758), 유전자등록번호(Genebank) AK094407(CDNA FLJ37088 fis, clone BRACE2017124), 유전자등록번호(Genebank) AK097139(unnamed protein product; Homo sapiens cDNA FLJ39820 fis, clone SPLEN2010625.), 유전자등록번호(Genebank)AK124402(Similar to Olfactory receptor 4F3), 유전자등록번호(Genebank) AK024680(CDNA: FLJ21027 fis, clone CAE07110), 유전자등록번호(Genebank) AF040248(EDRF2; Homo sapiens erythroid differentiation-related factor 2 mRNA, partial cds.), 유전자등록번호(Genebank) S79672, 유전자등록번호(Genebank) AA995107[Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor(Pan troglodytes)], 유전자등록번호(Genebank) AI742278(Transcribed locus), 유전자등록번호(Genebank) AK124873[MRNA; cDNA DKFZp547G036(from clone DKFZp547G036)], 유전자등록번호(Genebank) CR936602[MRNA; cDNA DKFZp686L1223(from clone DKFZp686L1223)], 유전자등록번호(Genebank) BX091356(Surfactant associated protein F mRNA, partial sequence), 유전자등록번호(Genebank)AF147727(Homo sapiens unknown mRNA.), 유전자등록번호(Genebank) BC018035(CDNA clone IMAGE:4819956), 유전자등록번호(Genebank) CR622298[HMT1 hnRNP methyltransferase-like 2(S. cerevisiae)], 유전자등록번호(Genebank) NM_153456(Heparan sulfate 6-O-sulfotransferase 3), 유전자등록번호(Genebank) AL834347(Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta- isomerase 7), 유전자등록번호(Genebank) NM_014157(HSPC065 protein), 유전자등록번호(Genebank) AL713654[pantothenate kinase 2(Hallervorden-Spatz syndrome)], 유전자등록번호(Genebank) NM_213621[5-hydroxytryptamine(serotonin) receptor 3A], 유전자등록번호(Genebank) BG284570(Transcribed locus), 유전자등록번호(Genebank) BC026338(Intercellular adhesion molecule 5, telencephalin), 유전자등록번호(Genebank) NM_015259(Inducible T-cell co-stimulator ligand), 유전자등록번호(Genebank) BM906235(Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), 유전자등록번호(Genebank) NM_014002(Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon), 유전자등록번호(Genebank) NM_000575(Interleukin 1, alpha), 유전자등록번호(Genebank) BC008678(Interleukin 1, beta), 유전자등록번호(Genebank) NM_000600[Interleukin 6(interferon, beta 2)], 유전자등록번호(Genebank) NM_178229(IQ motif containing GTPase activating protein 3), 유전자등록번호(Genebank) U52682(Interferon regulatory factor 4), 유전자등록번호(Genebank) AB209622(IL2-inducible T-cell kinase), 유전자등록번호(Genebank) AL049799(KIAA0040), 유전자등록번호(Genebank) BC002872(KIAA0515), 유전자등록번호(Genebank) AB028978(TBC1 domain family, member 2B), 유전자등록번호(Genebank) AB037748(KIAA1327 protein), 유전자등록번호(Genebank) NM_058237(KIAA1622), 유전자등록번XM_376018(KIAA1644 protein), 유전자등록번호(Genebank) XM_372193(KIAA1751), 유전자등록번호(Genebank)NM_015254(Kinesin family member 13B), 유전자등록번호(Genebank) AB007918(Kinesin family member 21B), 유전자등록번호(Genebank) NM_153443(Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1), 유전자등록번호(Genebank) AY302131(Kin of IRRE like(Drosophila)), 유전자등록번호(Genebank) BC033580[Kruppel-like factor 1(erythroid)], 유전자등록번호(Genebank) NM_001002231(Kallikrein 2, prostatic), 유전자등록번호(Genebank) NM_002260(Killer cell lectin-like receptor subfamily C, member 2), 유전자등록번호(Genebank) NM_012316[Karyopherin alpha 6(importin alpha 7)], 유전자등록번호(Genebank) BC063625(Keratin associated protein 2-4), 유전자등록번호(Genebank) BQ691496(Keratin associated protein 4-10), 유전자등록번호(Genebank) NM_000425(L1 cell adhesion molecule), 유전자등록번호(Genebank) NM_198129(Laminin, alpha 3), 유전자등록번호(Genebank) NM_178354(Late cornified envelope 1F), 유전자등록번호(Genebank) NM_002298[Lymphocyte cytosolic protein 1(L-plastin)], 유전자등록번호(Genebank) NM_002309(Hypothetical protein MGC20647), 유전자등록번호(Genebank) XM_292512(Hypothetical protein LOC146517), 유전자등록번호(Genebank) AK091946(Hypothetical protein LOC283337), 유전자등록번호(Genebank) BC035704(Hypothetical protein LOC284352), 유전자등록번호(Genebank) NM_001017995(KIAA1295), 유전자등록번호(Genebank) AK094356(Hypothetical protein LOC286382), 유전자등록번호(Genebank) XM_291200(Similar to BC060615 protein), 유전자등록번호(Genebank) BC103740(Low density lipoprotein receptor class A domain containing 1), 유전자등록번호(Genebank)XM_375549(Hypothetical LOC400664), 유전자등록번호(Genebank) XM_379036(Hypothetical LOC400890), 유전자등록번호(Genebank) XM_498732(Hypothetical protein LOC115110), 유전자등록번호(Genebank)AK125737(LOC440570), 유전자등록번호(Genebank) XM_496400(Similar to hypothetical protein KIAA0454-human(fragment)), 유전자등록번호(Genebank) BC094697(Hypothetical protein BC007901), 유전자등록번호(Genebank) NM_000237(Lipoprotein lipase), 유전자등록번호(Genebank) AK024955(Leucine rich repeat containing 19), 유전자등록번호(Genebank) AY358431(C-type lectin superfamily 4, member G), 유전자등록번호(Genebank) NM_002341[Lymphotoxin beta(TNF superfamily, member 3)], 유전자등록번호(Genebank) NM_000428(Latent transforming growth factor beta binding protein 2), 유전자등록번호(Genebank) AK024477(Latent transforming growth factor beta binding protein 3), 유전자등록번호(Genebank) NM_005461[V-maf musculoaponeurotic fibrosarcoma oncogene homolog B(avian)], 유전자등록번호(Genebank) AK092414[V-maf musculoaponeurotic fibrosarcoma oncogene homolog K(avian)], 유전자등록번호(Genebank) NM_138703(Melanoma antigen family E, 2), 유전자등록번호(Genebank) CR749534(Mannosidase, alpha, class 1B, member 1), 유전자등록번호(Genebank) NM_005764(PDZK1 interacting protein 1), 유전자등록번호(Genebank) NM_002375(Microtubule-associated protein 4), 유전자등록번호(Genebank) NM_030583(Matrilin 2), 유전자등록번호(Genebank) CR627018(Myelin basic protein), 유전자등록번호(Genebank) AK058070[Malate dehydrogenase 1B, NAD(soluble)], 유전자등록번호(Genebank) AK000052[Mediator of RNA polymerase II transcription, subunit 18 homolog(yeast)], 유전자등록번호(Genebank) AK128256[MADS box transcription enhancer factor 2, polypeptide B(myocyte enhancer factor 2B)], 유전자등록번호(Genebank) NM_005929[Antigen p97(melanoma associated) identified by monoclonal antibodies 133.2 and 96.5], 유전자등록번호(Genebank) AB235153[Mannosyl(alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B], 유전자등록번호(Genebank)AK075342(Hypothetical protein MGC10946), 유전자등록번호(Genebank) BC002462(Coiled-coil domain containing 28B), 유전자등록번호(Genebank) BC009980(Kelch domain containing 7B), 유전자등록번호(Genebank) BX647942(Likely ortholog of mouse schlafen 5), 유전자등록번호(Genebank) AY358435(Hypothetical protein MGC21688), 유전자등록번호(Genebank) BC004968(Hypothetical protein MGC2664), 유전자등록번호(Genebank) AK125168(Hypothetical protein MGC5356), 유전자등록번호(Genebank) NM_001620[AHNAK nucleoprotein(desmoyokin)], 유전자등록번호(Genebank) NM_014632(Microtubule associated monoxygenase, calponin and LIM domain containing 2), 유전자등록번호(Genebank) S77878, 유전자등록번호(Genebank) NM_003482(Myeloid/lymphoid or mixed-lineage leukemia 2), 유전자등록번 호(Genebank) U38320(Matrix metallopeptidase 19), 유전자등록번호(Genebank) NM_004530[Matrix metallopeptidase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)], 유전자등록번호(Genebank) AJ298317(Mucin 5, subtypes A and C, tracheobronchial/gastric), 유전자등록번호(Genebank) NM_002471[Myosin, heavy polypeptide 6, cardiac muscle, alpha(cardiomyopathy, hypertrophic 1)], 유전자등록번호(Genebank)AB209997(Myosin IXB), 유전자등록번호(Genebank) NM_005967[NGFI-A binding protein 2(EGR1 binding protein 2)], 유전자등록번호(Genebank) AY358815(Neural cell adhesion molecule 1), 유전자등록번호(Genebank) AB082526[NIMA(never in mitosis gene a)- related kinase 9], 유전자등록번호(Genebank)AK055224(Neurogenic differentiation 6), 유전자등록번호(Genebank) NM_006162(Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1), 유전자등록번호(Genebank)AK090459(Nuclear factor(erythroid-derived 2)-like 1), 유전자등록번호(Genebank) AK074071(Sperm equatorial segment protein 1), 유전자등록번호(Genebank) AK160377(Nuclear pore complex interacting protein), 유전자등록번호(Genebank) NM_003873(Neuropilin 1), 유전자등록번호(Genebank) AL833606(Neuropilin 2), 유전자등록번호(Genebank) NM_001007156(Neurotrophic tyrosine kinase, receptor, type 3), 유전자등록번호(Genebank) NM_015231(Nucleoporin 160kDa), 유전자등록번호(Genebank) BC101462(Neurexophilin 2), 유전자등록번호(Genebank) XM_371717[Odz, odd Oz/ten-m homolog 3(Drosophila)], 유전자등록번호(Genebank) AI791380[Transcribed locus, weakly similar to NP_079012.2 gasdermin domain containing 1; 1810036L03Rik(Homo sapiens)], 유전자등록번호(Genebank) BC051352(Oligodendrocyte transcription factor 3), 유전자등록번호(Genebank) NM_001004751(Olfactory receptor, family 51, subfamily D, member 1), 유전자등록번호(Genebank) BG284742[P8 protein(candidate of metastasis 1)], 유전자등록번호(Genebank) NM_001618[Poly(ADP-ribose) polymerase family, member 1], 유전자등록번호(Genebank) BC035485[Poly(ADP-ribose) polymerase family, member 10], 유전자등록번호(Genebank) NM_032402(Protocadherin gamma subfamily A, 12), 유전자등록번호(Genebank) XM_374898[Pecanex-like 3(Drosophila)], 유전자등록번호(Genebank) AK001872(Programmed cell death 1 ligand 2), 유전자등록번호(Genebank)AB209081[Phosphodiesterase 4B, cAMP-specific(phosphodiesterase E4 dunce homolog, Drosophila)], 유전자등록번호(Genebank) AB094096(PDZ domain containing 2), 유전자등록번호(Genebank) AK127016(PDZ domain containing 4), 유전자등록번호(Genebank) BX648284[Pelota homolog(Drosophila)], 유전자등록번호(Genebank) AF289554(PERQ amino acid rich, with GYF domain 1), 유전자등록번호(Genebank) NM_006212(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2), 유전자등록번호(Genebank) NM_003629[Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)], 유전자등록번호(Genebank) NM_013440(Paired immunoglobin-like type 2 receptor beta), 유전자등록번호(Genebank) NM_052892(Polycystic kidney disease 1-like 2), 유전자등록번호(Genebank) NM_201382(Plectin 1, intermediate filament binding protein 500kDa), 유전자등록번호(Genebank) NM_032882(Paraneoplastic antigen like 6A), 유전자등록번호(Genebank) NM_00093[Polymerase(RNA) II(DNA directed) polypeptide A, 220kDa], 유전자등록번호(Genebank) NM_000944[(Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)], 유전자등록번호(Genebank) BQ073453(Protein kinase C, delta binding protein), 유전자등록번호(Genebank) NM_014502[PRP19/PSO4 pre-mRNA processing factor 19 homolog(S. cerevisiae)], 유전자등록번호(Genebank) M23575(Pregnancyspecific beta-1-glycoprotein 3), 유전자등록번호(Genebank) NM_002825[Pleiotrophin(heparin binding growth factor 8, neurite growth-promoting factor 1)], 유전자등록번호(Genebank) NM_002843(Protein tyrosine phosphatase, receptor type, J), 유전자등록번호(Genebank) NM_002852(Pentraxin-related gene, rapidly induced by IL-1 beta), 유전자등록번호(Genebank) BC017045(Phosphorylase, glycogen; brain), 유전자등록번호(Genebank)BM974642(Transcribed locus), 유전자등록번호(Genebank) NM_002826(Quiescin Q6), 유전자등록번호(Genebank)NM_181701(Quiescin Q6-like 1), 유전자등록번호(Genebank) AF329499(RAB27B, member RAS oncogene family), 유전자등록번호(Genebank) CR620019(RAB43, member RAS oncogene family), 유전자등록번호(Genebank) NM_198679[Rap guanine nucleotide exchange factor(GEF) 1], 유전자등록번호(Genebank) NM_002888[Retinoic acid receptor responder(tazarotene induced) 1], 유전자등록번호(Genebank) AK092804[Retinoic acid receptor responder(tazarotene induced) 2], 유전자등록번호(Genebank) X89399(RAS p21 protein activator 3), 유전자등록번호(Genebank) NM_002891(Ras protein-specific guanine nucleotide-releasing factor 1), 유전자등록번호(Genebank) NM_002891(Ras protein-specific guanine nucleotide-releasing factor 1), 유전자등록번호(Genebank) AK128127(Regulator of G-protein signalling 3), 유전자등록번호(Genebank) BM980591(Transcribed locus), 유전자등록번호(Genebank) BG564669(Serum amyloid A2), 유전자등록번호(Genebank) NM_030754(Serum amyloid A2), 유전자등록번호(Genebank) BG564326(Serum amyloid A4, constitutive), 유전자등록번호(Genebank) BC022509[Secretogranin II(chromogranin C)], 유전자등록번호(Genebank) NM_006514(Sodium channel, voltage-gated, type X, alpha), 유전자등록번호(Genebank) NM_198056[Sodium channel, voltage-gated, type V, alpha(long QT syndrome 3)], 유전자등록번호(Genebank) AB007937[Syndecan 3(N-syndecan)], 유전자등록번호(Genebank) NM_002999[Syndecan 4(amphiglycan, ryudocan)], 유전자등록번호(Genebank) AK122782(Orphan short-chain dehydrogenase / reductase), 유전자등록번호(Genebank) NM_003004(Secreted and transmembrane 1), 유전자등록번호(Genebank) NM_152754[Sema domain, immunoglobulin domain(Ig), short basic domain, secreted,(semaphorin) 3D], 유전자등록번호(Genebank) AK093049[Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3], 유전자등록번호(Genebank) AK058094(Surfactant, pulmonary-associated protein C), 유전자등록번호(Genebank) NM_144579(Sideroflexin 5), 유전자등록번호(Genebank) AK074131(SH3 multiple domains 4), 유전자등록번호(Genebank) BC098390[Solute carrier family 12(potassium/chloride transporters), member 7], 유전자등록번호(Genebank) AK091926(Solute carrier family 13(sodium-dependent dicarboxylate transporter), member 2), 유전자등록번호(Genebank) AK127216(Solute carrier family 15, member 3), 유전자등록번호(Genebank) NM_004172[Solutecarrier family 1(glial high affinity glutamate transporter], member 3), 유전자등록번호(Genebank) NM_153320[Solute carrier family 22(organic anion transporter), member 7], 유전자등록번호(Genebank) NM_203327[Solute carrier family 23(nucleobase transporters), member 2], 유전자등록번호(Genebank) NM_152346(Solute carrier family 43, member 2), 유전자등록번호(Genebank) NM_201649[Solute carrier family 6(neurotransmitter transporter, glycine), member 9], 유전자등록번호(Genebank) NM_003045[Solute carrier family 7(cationic amino acid transporter, y+ system), member 1], 유전자등록번호(Genebank) NM_003486[Solute carrier family 7(cationic amino acid transporter, y+ system), member 5], 유전자등록번호(Genebank) BC098388[Slit homolog 3(Drosophila)], 유전자등록번호(Genebank)NM_139067(SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2), 유전자등록번호(Genebank) AK127286(Spermine oxidase), 유전자등록번호(Genebank) NM_003955(Suppressor of cytokine signaling 3), 유전자등록번 호(Genebank) NM_003105(Chromosome 11 open reading frame 32), 유전자등록번호(Genebank) NM_003106[SRY(sex determining region Y)-box 2], 유전자등록번호(Genebank) NM_173360(Spermatogenesis associated 9), 유전자등록번호(Genebank) BC017222(Sequestosome 1), 유전자등록번호(Genebank) NM_080836(Serine/threonine kinase 35), 유전자등록번호(Genebank) AF545571(Sulfatase 1), 유전자등록번호(Genebank) NM_177963(Synaptotagmin XII), 유전자등록번호(Genebank) NM_000545[Transcription factor 1, hepatic; LF-B1, hepatic nuclear factor(HNF1), albumin proximal factor], 유전자등록번호(Genebank) NM_000358(Transforming growth factor, beta-induced, 68kDa), 유전자등록번호(Genebank) NM_015335(Thyroid hormone receptor associated protein 2), 유전자등록번호(Genebank) AB046767[Transducin-like enhancer of split 3(E(sp1) homolog, Drosophila)], 유전자등록번호(Genebank) AF367761(Transmembrane protein 10), 유전자등록번호(Genebank) NM_002160(Tenascin C(hexabrachion)), 유전자등록번호(Genebank) BC028148[Tumor necrosis factor(TNF superfamily, member 2)], 유전자등록번호(Genebank) NM_006291(Tumor necrosis factor, alpha-induced protein 2), 유전자등록번호(Genebank) NM_006290(Tumor necrosis factor, alpha-induced protein 3), 유전자등록번호(Genebank) NM_007115(Tumor necrosis factor, alpha-induced protein 6), 유전자등록번호(Genebank) BC052977(Tumor necrosis factor receptor superfamily, member 1B), 유전자등록번호(Genebank) NM_003811[Tumor necrosis factor(ligand) superfamily, member 9], 유전자등록번호(Genebank) AB210011[Transportin 2(importin 3, karyopherin beta 2b)], 유전자등록번호(Genebank) NM_005427(Tumor protein p73), 유전자등록번호(Genebank) AB032995(Two pore segment channel 1), 유전자등록번호(Genebank) S76980[hexosaminidase A(alpha polypeptide)], 유전자등록번호(Genebank)NM_003320[Tubby homolog(mouse)], 유전자등록번호(Genebank) NM_003328(TXK tyrosine kinase), 유전자등록번호(Genebank) NM_006472(Thioredoxin interacting protein), 유전자등록번호(Genebank)NM_003360[UDP glycosyltransferase 8(UDP-galactose ceramide galactosyltransferase)], 유전자등록번호(Genebank) NM_025154[Unc-84 homolog A(C. elegans)], 유전자등록번호(Genebank)BC094797[Unc-84 homolog B(C. elegans)], 유전자등록번호(Genebank) AW016806(Transcribed locus), 유전자등록번호(Genebank) AW341701(Transcribed locus), 유전자등록번호(Genebank) NM_199511(Steroid sensitive gene 1), 유전자등록번호(Genebank) DQ097177(HECT, UBA and WWE domain containing 1), 유전자등록번호(Genebank) BC027954(Tcell receptor gamma variable 7), 유전자등록번호(Genebank) NM_003371(Vav 2 oncogene), 유전자등록번호(Genebank) AF167081(Variable charge, X-linked), 유전자등록번호(Genebank) AK058041[Vestigial like 4(Drosophila)], 유전자등록번호(Genebank) AJ132099(Vanin 1), 유전자등록번호(Genebank) NM_014588[Visual system homeobox 1 homolog, CHX10-like(zebrafish)], 유전자등록번호(Genebank) NM_000638[Vitronectin(serum spreading factor, somatomedin B, complement S-protein)], 유전자등록번호(Genebank) NM_022170(Myosin XVB pseudogene), 유전자등록번호(Genebank) NM_032951(MLX interacting protein-like), 유전자등록번호(Genebank) BC052234(Wingless-type MMTV integration site family, member 10A), 유전자등록번호(Genebank) AK126706(X-ray repair complementing defective repair in Chinese hamster cells 3), 유전자등록번호(Genebank) NM_020205(Zinc finger, A20 domain containing 1), 유전자등록번호(Genebank) D26122(splicing factor 1), 유전자등록번호(Genebank) NM_032441(Zinc finger, matrin type 1), 유전자등록번호(Genebank) BC036823(Zinc finger protein 289, ID1 regulated), 유전자등록번호(Genebank) NM_182975(Zinc finger protein 326), 유전자등록번호(Genebank) NM_181489(Zinc finger protein 445), 유전자등록번호(Genebank) BG201273[Zinc finger protein 75(D8C6)], 유전자등록번호(Genebank)AY008294[Zinc finger protein, subfamily 1A, 2(Helios)].Genebank BE378852 (Transcribed locus), Genebank BG527520 [Transcribed locus, moderately similar to XP_540962. 1 PREDICTED: similar to centrin 4 (Canis familiaris)], Genebank NM_004797 (Adiponectin, C1Q and collagen domain containing), Genebank AF153821 [Alcohol dehydrogenase IB (class I), beta polypeptide] Genebank NM_006818 (Myeloid / lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 11), Genebank BP871540 (Transcribed locus), Genebank AF269088 (Genebank) Ankyrin repeat domain 30B), gene registration number (Genebank) BC018064 (Activating transcription factor 6), gene registration number (Genebank) XM_208270 (Acrosin-like protease), gene registration number (Genebank) NM_004323 (BCL2-associated athanogene), gene registration Number (Genebank) BE816155 (RC0-BN0198-220500-021-f04 BN0198 Homo sapiens cDNA, mRNA sequence. ), Genebank NM_003670 (Basic helix-loop-helix domain containing, class B, 2), Genebank BE614991 [Transcribed locus, strongly similar to XP_514491. 1 PREDICTED: similar to hypothetical protein (Pan troglodytes), Genebank CN484301 [Transcribed locus, moderately similar to XP_227769. 2 PREDICTED: similar to Ac1147 (Rattus norvegicus)], Genebank AK027152 (Chromosome 11 open reading frame 1), Genebank XM_290629 (Chromosome 14 open reading frame 78), Genebank (Genebank) NM_173817 (Chromosome 18 open reading frame 58), Genebank CR625148 (Hypothetical LOC541469 protein), Genebank BX537504 (Complement component 2), Genebank BX281604 (Chromosome 20 open reading frame 187), Genebank BU618438 (Chromosome 20 open reading frame 77), Genebank (Genebank) BC020888 (Metallophosphoesterase domain containing 1), Genebank (Genebank) NM_213720 (Chromosome 22 open reading frame 16), gene Genebank BC027919 (Chromosome 5 open reading frame 16), Genebank NM_030568 (Chromosome 6 open reading frame 147), Genebank AK021476 (Chromosome 6 open reading frame 164), gene Genebank BC036421 (Chromosome 9 open reading frame 103), Genebank NM_153707 (Chromosome 9 open reading frame 138), Genebank NM_203403 (Chromosome 9 open reading frame 150), Gene accession number (Genebank) NM_021208 (Chromosome 9 open reading frame 27), Gene Registration Number (Genebank) NM_053056 (Cyclin D1), Gene Registration Number (Genebank) NM_001295 [Chemokine (CC motif) receptor 1], Gene Registration Number (Genebank) AK126349 ( CD200 receptor 1), Genebank AK026467 (CD22 antigen), Genebank AK054921 (Cerebellar degeneration-related protein 1, 34kDa), Genebank NM_012125 (Cholinergic receptor, muscarinic 5), Genebank (Genebank) NM_003992 (CDC-like kinase 3), Genebank (Genebank) AK022155 (Coproporphyrinogen oxidase), Genebank (Kenebank) AK090482 [Chemokine (CXC motif) ligand 12 (stromal cell-derived factor 1) ], Genebank NM_1 52693 (Chromosome X open reading frame 50), Genebank BX108307 (Defensin, beta 126), Genebank AB051529 [Dispatched homolog 2 (Drosophila)], Genebank BC014951 (chromosome 20 open reading frame 107), Genebank AK095541 [DnaJ (Hsp40) homolog, subfamily A, member 1], Genebank (Genebank) BC036806 (Extracellular matrix protein 2, female organ and adipocyte specific), Gene accession number (Genebank) BI830993 (Eukaryotic translation initiation factor 4E), Gene Registration Number (Genebank) BX537939 [Elastin (supravalvular aortic stenosis, Williams-Beuren syndrome)], Gene Registration Number (Genebank) AB051895 (Epiplakin 1), Gene Registration Number (Genebank) ) AB209361 [Fas (TNF receptor superfamily, member 6)], Genebank NM_033480 (F-box protein 9), Genebank (Genebank) BC034763 (Ferredoxin reductase), Genebank (Menebank) NM_005141 (Fibrinogen beta chain), gene registration number (Genebank) AL832445 (Hypothetical protein FLJ25770), Genebank XM_499008 (Hypothetical gene supported by AK124699), Genebank AK124499 (RGS9 anchor protein), Genebank CR936597 (Fibrous sheath interacting protein 2), gene registration number (Genebank) NM_004293 (Guanine deaminase), gene registration number (Genebank) NM_177483 (Glycosylphosphatidylinositol specific phospholipase D1), gene registration number (Genebank) AK074729 (G protein-coupled receptor 19), gene registration number ( Genebank) AK122626 (G protein-coupled receptor 82), Genebank NM_014619 (Glutamate receptor, ionotropic, kainate 4), Genebank NM_002133 [Heme oxygenase (decycling) 1], Genebank (Genebank) CB124304 [Transcribed locus, strongly similar to NP_001604. 1 alpha 2 actin; alpha-cardiac actin (Homo sapiens)], Genebank BE745738 (CDNA clone IMAGE: 3928921), Gene Registration Number (Genebank) BC043212 (Hypothetical LOC401057), Gene Registration Number (Genebank) BC008624 (Homo sapiens cDNA clone MGC : 18299 IMAGE: 4179890, complete cds. ), Genebank AK024336 (Homo sapiens cDNA FLJ14274 fis, clone PLACE1004979. ), Gene registration number (Genebank) AK057952 (CDNA FLJ25223 fis, clone STM00880), gene registration number (Genebank) AK123483 (CDNA FLJ41489 fis, clone BRTHA2004582), gene registration number (Genebank) BU570278 [Similar to dJ1153D9. 4 (novel protein)], Genebank BE614991 [Transcribed locus, strongly similar to XP_514491. 1 PREDICTED: similar to hypothetical protein (Pan troglodytes)], Genebank T77197 (TBC1 domain family, member 3B), Genebank AF119839 (predicted protein of HQ0889; Homo sapiens PRO0889 mRNA, complete cds. ), Genebank AF119912 (predicted protein of HQ3073; Homo sapiens PRO3073 mRNA, complete cds. ), Genebank BM467031 (Ribosomal protein S2), Genebank CA943742 [Transcribed locus, strongly similar to XP_429266. 1 PREDICTED: hypothetical protein XP_429266 (Gallus gallus)], Genebank AV727525 (Transcribed locus), Genebank BC015351 (Homo sapiens, clone IMAGE: 4420333, mRNA), Genebank BG759321 Homo sapiens, clone IMAGE: 4852110, mRNA, Genebank BF337308 (Homeodomain-only protein), Genebank AK223249 (Homeo box B7), Genebank NM_021192 (Homeo box D11 ), Gene registration number (Genebank) NM_005345 (Heat shock 70kDa protein 1A), gene registration number (Genebank) NM_006597 (Heat shock 70kDa protein 8), gene registration number (Genebank) U88896, gene registration number (Genebank) NM_134470 (Interleukin 1 receptor accessory protein), Genebank BC069137 [Interleukin 5 (colony-stimulating factor, eosinophil)], Genebank AB039920 (KCNQ1 downstream neighbor), Genebank AB033055 (Outer dense fiber of sperm tails 2-like) Number (Genebank) AK001632 (Hypothetical protein FLJ10770), gene registration number (Genebank) BC042437 [Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)], gene registration number (Genebank) BX647923 (Keratin 17), gene registration number ( Genebank) BC025396 [Spermatogenesis associated 18 homolog (rat)], Genebank BC016613 (Similar to hypothetical protein FLJ13841), Genebank BU536672 (Ribosomal protein L22-like 1), Genebank BC012203 (Hypothetical protein LOC284361), Genebank BC044311 (Hypothetical protein LOC339344), Genebank AK022806 (Hypothetical protein LOC340281), Genebank XM_371643 (Similar to hypothetical protein, Gene registration MGC39 Number (Genebank) XM_372625 (similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author, Genebank XM_498623 (LOC440309), Genebank XM_496818 (similar to RIKEN cDNA 2410146L05), Genebank XM_497458 (Similar to large subunit ribosomal protein L36a ), Genebank AK127395 (Ankyrin repeat domain 37), Genebank AK126961 (Leucine rich repeat transmembrane neuronal 4), Genebank AK026984 (Multiple C2 domains, transmembrane 2), Gene registration Genebank BE644770 (Mitofusin 1), Genebank AK123160 (Chromosome 1 open reading frame 162), Genebank AK125877 (Hypothetical protein MGC27016), Genebank AK124107 (Leucine rich repeat containing 43), Genebank AK097334 (Hypothetical protein MGC45840), Genebank AK123482 (Microsomal glutathione S-transferase 1), Genebank AK128769 (calcium c) hannel, voltage-dependent, beta 2 subunit), Genebank AY154467 (NACHT, leucine rich repeat and PYD containing 12), Genebank AK124709 (N-myc downstream regulated gene 1), Gene Registration Number (Genebank) NM_016931 (NADPH oxidase 4), Genebank BX640730 (Nebulin-related anchoring protein), Genebank (Genebank) AA524504 (Full length insert cDNA clone ZE12C10), Genebank NM_002563 (Purinergic receptor P2Y, G-protein coupled, 1), Genebank number AL834134 (Protocadherin 15), Genebank number (Genebank) NM_001011515 (PDZ and LIM domain 5), Genebank number S81916 (Phosphoglycerate kinase 1) , Genebank NM_003662 [Pirin (iron-binding nuclear protein)], Genebank (Genebank) NM_033256 [Protein phosphatase 1, regulatory (inhibitor) subunit 14A], Genebank (Menebank) NM_014068 (Psoriasis susceptibility 1 candidate 1), Gene registration number (Genebank) NM_002900 (Retinol binding protein 3, interstitial), Genebank AK055999 (Ret finger protein-like 2), Genebank AB209019 (Regulator of G-protein signaling 4), Gene registration number ( Genebank) NM_004040 (Ras homolog gene family, member B), Genebank (Genebank) BU902070 (Ribosomal protein L36a-like), Genebank (Genebank) BF983391 (Ribosomal protein S27-like), Gene Registration Number (Genebank) NM_173640 R-spondin homolog (Xenopus laevis), Genebank BG182941 [Transcribed locus, weakly similar to XP_510104. 1 PREDICTED: similar to hypothetical protein FLJ25224 (Pan troglodytes)], Genebank NM_004057 (S100 calcium binding protein G), Genebank BC041022 (SCAN domain containing 1), Genebank NM_002639 [Serpin peptidase inhibitor, clade B (ovalbumin), member 5], Genebank NM_005668 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4), Genebank NM_003830 ( Sialic acid binding Ig-like lectin 5), Genebank BC053934 [Sirtuin (silent mating type information regulation 2 homolog) 4 (S.  cerevisiae)], Genebank NM_052832 (Solute carrier family 26, member 7), Genebank NM_014332 (Small muscle protein, X-linked), Genebank BC026316 [Suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein)], Genebank AK122707 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3), Genebank AK026585 (Tumor-associated calcium signal transducer 1) , Genebank AL038125 (Zinc finger, AN1-type domain 3), Genebank (Genebank) NM_000361 (Thrombomodulin), Genebank (Genebank) BC010553 (TIMP metallopeptidase inhibitor 4), Genebank (Genebank) NM_003840 (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), Genebank NM_004881 (Tumor protein p53 inducible protein 3), Genebank NM_033229 (Tripartite motif-containing 15), gene Enrollment Genebank BC063872 (Tripartite motif-containing 9), Genebank NM_003314 (Tetrictricopeptide repeat domain 1), Genebank AK024002 (Tubulin, alpha 4), Genebank NM_006087 (Tubulin , beta 4), Genebank AF219946 (Tubby like protein 4), Genebank AK025742 [Uncoupling protein 2 (mitochondrial, proton carrier)], Genebank AI699331 (Transcribed locus), Genebank AF055634 [Unc-5 homolog C (C.  elegans)], Genebank AK023940 (Homo sapiens cDNA FLJ13878 fis, clone THYRO1001411), Genebank BE815283 (Transcribed locus), Genebank BM913228 [Full-length cDNA clone CS0DItaYI20 of Placen Cot 25-normalized of Homo sapiens (human)], Genebank BX099682 (Transcribed locus), Genebank BX640772 [MRNA; cDNA DKFZp686P02129 (from clone DKFZp686P02129)], Genebank AK094191 (Chromosome 8 open reading frame 34), Gene Registration Number (Genebank) NM_033400 (Zinc finger homeobox 2), Gene Registration Number (Genebank) XM_040592 (Zinc finger protein) 469), Genebank NM_007313 (V-abl Abelson murine leukemia viral oncogene homolog 1), Genebank NM_203380 (Acyl-CoA synthetase long-chain family member 5), Genebank BC040531 (Activin A receptor, type IB), Genebank Y13786 [ADAM metallopeptidase domain 19 (meltrin beta)], Genebank number AJ250725 (ADAM metallopeptidase with thrombospondin type 1 motif, 12), Gene registration number ( Genebank) AJ315733 (ADAM metallopeptidase with thrombospondin type 1 motif, 15), Gene Registration Number (Genebank) NM_133638 (ADAM metallopeptidase with thrombospondin type 1 motif, 19), Gene Registration Number (Genebank) NM_014243 (ADAM metallopeptidase with thrombospondi n type 1 motif, 3), Genebank NM_207517 (ADAMTS-like 3), Genebank (Genebank) BC013780 (Adenosine A2a receptor), Genebank AK160382 (AT-hook transcription factor), Genebank AK122839 (V-akt murine thymoma viral oncogene homolog 2), Genebank AK128709 (Aldehyde dehydrogenase 1 family, member A2), Genebank NM_003748 (Aldehyde dehydrogenase 4 family, member A1), Genebank NM_172196 (TFIIA-alpha / beta-like factor), Genebank NM_152526 [Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 19], Genebank NM_139314 (Angiopoietin-like 4), Genebank NM_000037 (Ankyrin 1, erythrocytic), Genebank AJ583444 [Ankyrin repeat domain 2 (stretch responsive muscle)], Genebank NM_145641 (Apolipoprotein L , 3), Genebank BC006276 ( Apolipoprotein L, 4), Genebank NM_003224 (ADP-ribosylation factor related protein 1), Genebank NM_021069 (Sorbin and SH3 domain containing 2), Genebank AK090447 (Rho GTPase activating protein 19), Genebank NM_015071 (Rho GTPase activating protein 26), Genebank NM_032199 [AT rich interactive domain 5B (MRF1-like)], Genebank NM_183376 (Arrestin domain containing) 4), Genebank AK000643 (Ankyrin repeat and SOCS box-containing 9), Gene Registration Number (Genebank) AB209032 (Activating transcription factor 3), Gene Registration Number (Genebank) NM_032827 [Atonal homolog 8 (Drosophila) ], Genebank BX537444 (ATPase, Ca ++ transporting, plasma membrane 4), Genebank (Genebank) NM_006045 (ATPase, Class II, type 9A), Genebank AB011106 (Attractin-like 1) , Gene Registration Number (Genebank) NM_000707 (Arginin e vasopressin receptor 1B), genebank (Genebank) CR933657 [Axin 2 (conductin, axil)], genebank (Genebank) NM_152384 (Bardet-Biedl syndrome 5), genebank (Genebank) NM_021948 (Brevican), gene Genebank AA608624 (Breast carcinoma amplified sequence 3), Gene Registration Number (Genebank) NM_017745 (BCL6 co-repressor), Gene Registration Number (Genebank) S72478, Genebank NM_001710 (B-factor, properdin) , Genebank CR749373 (chromosome 1 open reading frame 178), Genebank AL713697 (Bridging integrator 1), Genebank NM_001165 (Baculoviral IAP repeat-containing 3), Gene registry number ( Genebank) BM984396 (Plasminogen-like B2), Genebank NM_001203 (Bone morphogenetic protein receptor, type IB), Genebank AK098095 (V-raf murine sarcoma viral oncogene homolog B1), Gene Registration Number ( Genebank) NM_014299 (Bromodomain containing 4), Electronic registration number (Genebank) BC016743 [BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S.  cerevisiae)], Genebank NM_001728 [Basigin (OK blood group)], Genebank NM_052876 [BTB (POZ) domain containing 14B], Genebank BX537534 [BTB (POZ) domain containing 9], Genebank BX107768 (Transcribed locus), Genebank AY665468 [Ras association (RalGDS / AF-6) domain family 8], Genebank NM_033513 (Chromosome 19 open reading frame 20), Genebank BC068439 (Chromosome 19 open reading frame 28), Genebank AY358424 (C1q and tumor necrosis factor related protein 1), Genebank NM_001733 (Complement component 1, r subcomponent, Genebank NM_080834 (Chromosome 20 open reading frame 152), Genebank AK000652 (Chromosome 20 open reading frame 57), Genebank BC063852 (Complement component 3), Gene Registration Number (Genebank) AF251038 (Chromosome 5 open readi ng frame 5), Genebank BX640641 (Chromosome 6 open reading frame 107), Genebank (Genebank) AL832416 (Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1), Genebank (Genebank) ) BC021552 (Calcium channel, voltage-dependent, gamma subunit 6), Genebank number AL117636 (Calneuron 1), Genebank number (Genebank) AJ519841 (Calmodulin regulated spectrin-associated protein 1), Genebank number (Genebank) AK128124 (Calpain 5), Genebank NM_002982 [Chemokine (CC motif) ligand 2], Genebank BC020698 [Chemokine (CC motif) ligand 20], Genebank BC071718 [Chemokine (CC motif) ligand 4-like 1], gene registration number (Genebank) CR614015 (CD14 antigen), gene registration number (Genebank) NM_001004196 (CD200 antigen), gene registration number (Genebank) AK090470 [CD33 antigen (gp67)], Genebank (Genebank) NM_001773 (CD34 antigen), Gene Registration Number (Geneba) nk) BC025729 (CD99 antigen-like 2), gene registration number (Genebank) AB051503 (Centaurin, beta 5), gene registration number (Genebank) NM_032221 (Chromodomain helicase DNA binding protein 6), gene registration number (Genebank) NM_000740 (Cholinergic receptor, muscarinic 3), gene registration number (Genebank) NM_016369 (Claudin 18), gene registration number (Genebank) AB007969 [Calmin (calponin-like, transmembrane)], gene registration number (Genebank) AJ278018 (Calsyntenin 2), gene registration Genebank AJ626900 (Cardiomyopathy associated 1), Genebank NM_020872 [Contactin 3 (plasmacytoma associated)], Genebank NM_004370 (Collagen, type XII, alpha 1), Genebank (Genebank) Z74615 (Collagen, type I, alpha 1), Genebank NM_001845 (Collagen, type IV, alpha 1), Genebank NM_033380 [Collagen, type IV, alpha 5 (Alport syndrome)], gene Registration Number (Genebank) NM_000393 (Collagen, type V, alpha 2), Gene Registration Number (Gene bank) NM_001848 (Collagen, type VI, alpha 1), Genebank (Genebank) NM_001850 (Collagen, type VIII, alpha 1), Genebank (Genebank) AK124894 (Corticotropin releasing hormone receptor 1), Gene accession number (Genebank) ) BC021117 [Colony stimulating factor 1 (macrophage)], gene registration number (Genebank) BE873976 [Colony stimulating factor 2 (granulocyte-macrophage)], gene registration number (Genebank) NM_172220 [Colony stimulating factor 3 (granulocyte)], gene registration Number (Genebank) AK092391 (Cystatin E / M), Gene Registration Number (Genebank) NM_147780 (Cathepsin B), Gene Registration Number (Genebank) NM_001334 (Cathepsin O), Gene Registration Number (Genebank) NM_198700 (CUG triplet repeat, RNA binding protein 1), genebank NM_015267 [Cut-like 2 (Drosophila)], genebank (Genebank) NM_001511 [Chemokine (CXC motif) ligand 1 (melanoma growth stimulating activity, alpha)], gene registry number ( Genebank) BC053653 [Chemokine (CXC motif) ligand 2], gene registration Genebank NM_002090 [Chemokine (CXC motif) ligand 3], Genebank NM_002994 [Chemokine (CXC motif) ligand 5], Genebank U81234 [Chemokine (CXC motif) ligand 6 (granulocyte chemotactic protein 2)], Genebank NM_000497 (Cytochrome P450, family 11, subfamily B, polypeptide 1), Genebank NM_004393 [Dystroglycan 1 (dystrophin-associated glycoprotein 1)], Genebank (Genebank) ) BX537651 (Discoidin domain receptor family, member 2), Genebank AB209635 (Diacylglycerol kinase, zeta 104kDa), Genebank AB209653 [Dehydrogenase / reductase (SDR family) member 2], Gene registration number (Genebank) NM_004753 [Dehydrogenase / reductase (SDR family) member 3], Gene Registration Number (Genebank) AL832637 (Taxilin alpha), Gene Registration Number (Genebank) BX640676 (Regeneration associated muscle protease), Gene Registration Number (Genebank) XM_291222 ( Integrator complex subunit 1), heredity Genebank NM_031294 (Leucine rich repeat containing 48), Gene Registration Number (Genebank) NM_182643 (Deleted in liver cancer 1), Gene Registration Number (Genebank) NM_001365 [Discs, large homolog 4 (Drosophila)], Gene registration Genebank NM_007329 (Deleted in malignant brain tumors 1), Genebank BM808864 (Dopamine receptor D1 interacting protein), Genebank AK057845 (Ephrin-A1), Genebank NM_012199 ( Eukaryotic translation initiation factor 2C, 1), Genebank BX537368 [E74-like factor 3 (ets domain transcription factor, epithelial-specific)], Genebank NM_001426 (Engrailed homolog 1), Gene accession number (Genebank) BC054508 (Erythrocyte membrane protein band 4. 1 like 5), Gene Registration Number (Genebank) AF073727 (Epsin 1), Gene Registration Number (Genebank) BC060883 (Epsin 2), Gene Registration Number (Genebank) AL831953 [Epithelial stromal interaction 1 (breast)], Gene Registration Number ( Genebank) D79987 [Extra spindle poles like 1 (S.  cerevisiae)], Genebank NM_00198 [Ets variant gene 6 (TEL oncogene)], Genebank BX648769 (Ewing sarcoma breakpoint region 1), Genebank NM_014661 (Family with sequence similarity 53) , member B), Gene Registration Number (Genebank) NM_001004019 (Fibulin 2), Gene Registration Number (Genebank) AK125888 (F-box protein 32), Gene Registration Number (Genebank) NM_002016 (Filaggrin), Gene Registration Number (Genebank) NM_024669 (FLJ11795 protein), Genebank NM_024817 (Hypothetical protein FLJ13710), Genebank NM_017631 (Hypothetical protein FLJ20035), Genebank BQ279256 (Hypothetical protein FLJ20294), Genebank (Genebank) AK123641 (Cleavage and polyadenylation specific factor 3-like), Genebank AK024488 (Hypothetical protein FLJ21438), Genebank AL832659 (Hypothetical protein FLJ22833), Genebank NM_181645 (Hypothe tical protein FLJ25393), Genebank AL833626 (Zinc finger, DHHC-type containing 20), Genebank (Genebank) NM_173621 (Chromosome 17 open reading frame 44), Genebank NM_001460 (Flavin containing monooxygenase 2), Genebank NM_001461 (Flavin containing monooxygenase 5), Genebank NG_212482 (Fibronectin 1), Genebank AB033068 [Fizzy / cell division cycle 20 related 1 (Drosophila)], Genebank NM_080612 (GRB2-associated binding protein 3), Genebank BC051463 [Gamma-aminobutyric acid (GABA) B receptor, 1], Genebank NM_198321 [UDP-N-acetyl -alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)], Genebank NM_198335 (Glucosidase, alpha; neutral AB), Genebank NM_002048 (Growth arrest-specific 1), Genebank AK127845 (GATA binding protein 2), Genebank NM_000514 (Glial cell derived neurotrophic factor), Gene registration Number (Genebank) NM_000165 [Gap junction protein, alpha 1, 43 kDa (connexin 43)], gene registration number (Genebank) BC043390 (Glucagon-like peptide 2 receptor), gene registration number (Genebank) AF288389 (Glycosyltransferase 25 domain containing 2) , Genebank BC051726 [Glutamate-ammonia ligase (glutamine synthetase)], Gene accession number (Genebank) CR936770 [Guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O], Gene accession number (Genebank) BC095544 (G protein-coupled receptor 32), Genebank NM_000841 (Glutamate receptor, metabotropic 4), Genebank X99975 (H. sapiens mRNA for hRTR / hGCNF protein. ), Genebank CR624693 (3-hydroxyanthranilate 3,4-dioxygenase), Genebank AF227948 (Hepatitis B virus x associated protein), Genebank AL834223 (Histone deacetylase 11), Gene Genebank NM_021170 [Hairy and enhancer of split 4 (Drosophila)], Gene accession number (Genebank) AK075040 [Hairy and enhancer of split 6 (Drosophila)], Gene accession number (Genebank) BC101593 (Histone 1, H1a) Gene Registration Number (Genebank) BU603483 (Histone 1, H1e), Gene Registration Number (Genebank) BC065757 (CDNA clone IMAGE: 4556546), Gene Registration Number (Genebank) AK091904 (CDNA FLJ34585 fis, clone KIDNE2008758), Gene Registration Number (Genebank) AK094407 (CDNA FLJ37088 fis, clone BRACE2017124), Genebank AK097139 (unnamed protein product; Homo sapiens cDNA FLJ39820 fis, clone SPLEN2010625. ), Genebank No.AK124402 (Similar to Olfactory receptor 4F3), Genebank No. AK024680 (CDNA: FLJ21027 fis, clone CAE07110), Genebank AF040248 (EDRF2; Homo sapiens erythroid differentiation-related factor 2 mRNA, partial cds. ), Genebank S79672, Genebank AA995107 [Transcribed locus, moderately similar to XP_517807. 1 PREDICTED: interleukin 7 receptor (Pan troglodytes), Genebank AI742278 (Transcribed locus), Genebank AK124873 [MRNA; cDNA DKFZp547G036 (from clone DKFZp547G036)], Genebank CR936602 [MRNA; cDNA DKFZp686L1223 (from clone DKFZp686L1223)], Genebank BX091356 (Surfactant associated protein F mRNA, partial sequence), Genebank AF147727 (Homo sapiens unknown mRNA. ), Genebank BC018035 (CDNA clone IMAGE: 4819956), Genebank CR622298 [HMT1 hnRNP methyltransferase-like 2 (S.  cerevisiae)], Genebank NM_153456 (Heparan sulfate 6-O-sulfotransferase 3), Genebank AL834347 (Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7), Genebank NM_014157 (HSPC065 protein), Genebank AL713654 [pantothenate kinase 2 (Hallervorden-Spatz syndrome)], Genebank NM_213621 [5-hydroxytryptamine (serotonin) receptor 3A], gene Genebank BG284570 (Transcribed locus), Genebank (Genebank) BC026338 (Intercellular adhesion molecule 5, telencephalin), Genebank (Genebank) NM_015259 (Inducible T-cell co-stimulator ligand), Genebank (Genebank) ) BM906235 (Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), Genebank NM_014002 (Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon), Genebank NM_000575 (Interleukin 1, alpha), you Genebank BC008678 (Interleukin 1, beta), Genebank NM_000600 [Interleukin 6 (interferon, beta 2)], Genebank NM_178229 (IQ motif containing GTPase activating protein 3), Gene Registration Number (Genebank) U52682 (Interferon regulatory factor 4), Gene Registration Number (Genebank) AB209622 (IL2-inducible T-cell kinase), Gene Registration Number (Genebank) AL049799 (KIAA0040), Gene Registration Number (Genebank) BC002872 (KIAA0515 ), Gene registration number (Genebank) AB028978 (TBC1 domain family, member 2B), gene registration number (Genebank) AB037748 (KIAA1327 protein), gene registration number (Genebank) NM_058237 (KIAA1622), gene registration number XM_376018 (KIAA1644 protein), Genebank Number (Genebank) XM_372193 (KIAA1751), Gene Registration Number (Genebank) NM_015254 (Kinesin family member 13B), Gene Registration Number (Genebank) AB007918 (Kinesin family member 21B), Gene Registration Number (Genebank) NM_153443 (Killer cell immunoglobulin -like receptor, three domains, l ong cytoplasmic tail, 1), Genebank AY302131 (Kin of IRRE like (Drosophila)), Genebank (Genebank) BC033580 [Kruppel-like factor 1 (erythroid)], Genebank NM_001002231 ( Kallikrein 2, prostatic), Genebank NM_002260 (Killer cell lectin-like receptor subfamily C, member 2), Genebank NM_012316 [Karyopherin alpha 6 (importin alpha 7)], Genebank (Genebank) ) BC063625 (Keratin associated protein 2-4), Genebank (Genebank) BQ691496 (Keratin associated protein 4-10), Genebank (Genebank) NM_000425 (L1 cell adhesion molecule), Genebank (Genebank) NM_198129 (Laminin , alpha 3), Genebank NM_178354 (Late cornified envelope 1F), Genebank NM_002298 [Lymphocyte cytosolic protein 1 (L-plastin)], Genebank NM_002309 (Hypothetical protein MGC20647) , Genebank XM_292512 (Hypothetical protein LOC146517), Genebank Number AK091946 (Hypothetical protein LOC283337), Genebank Number (Genebank) BC035704 (Hypothetical protein LOC284352), Genebank Number NM_001017995 (KIAA1295), Genebank Number AK094356 protein (Hybank) LOC286382), Genebank XM_291200 (Similar to BC060615 protein), Genebank (Genebank) BC103740 (Low density lipoprotein receptor class A domain containing 1), Genebank XM_375549 (Hypothetical LOC400664), Gene registration Number (Genebank) XM_379036 (Hypothetical LOC400890), Gene Registration Number (Genebank) XM_498732 (Hypothetical protein LOC115110), Gene Registration Number (Genebank) AK125737 (LOC440570), Gene Registration Number (Genebank) XM_496400 (Similar to hypothetical protein KIAA0454-human fragments), Genebank BC094697 (Hypothetical protein BC007901), Genebank NM_000237 (Lipoprotein lipase), Genebank AK02 4955 (Leucine rich repeat containing 19), Genebank AY358431 (C-type lectin superfamily 4, member G), Genebank NM_002341 [Lymphotoxin beta (TNF superfamily, member 3)], Gene accession number (Genebank) NM_000428 (Latent transforming growth factor beta binding protein 2), Gene accession number (Genebank) AK024477 (Latent transforming growth factor beta binding protein 3), Gene accession number (Genebank) NM_005461 [V-maf musculoaponeurotic fibrosarcoma oncogene homolog B ( avian)], Genebank AK092414 [V-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)], Genebank NM_138703 (Melanoma antigen family E, 2), Genebank CR749534 (Mannosidase) , alpha, class 1B, member 1), Genebank NM_005764 (PDZK1 interacting protein 1), Genebank NM_002375 (Microtubule-associated protein 4), Genebank NM_030583 (Matrilin 2) , Genes, etc. Genebank CR627018 (Myelin basic protein), Genebank AK058070 [Malate dehydrogenase 1B, NAD (soluble)], Genebank AK000052 [Mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) ], Genebank AK128256 [MADS box transcription enhancer factor 2, polypeptide B (myocyte enhancer factor 2B)], Genebank NM_005929 [Antigen p97 (melanoma associated) identified by monoclonal antibodies 133. 2 and 96. 5], Genebank AB235153 [Mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B], Genebank AK075342 (Hypothetical protein MGC10946) , Genebank Number BC002462 (Coiled-coil domain containing 28B), Genebank Number (Genebank) BC009980 (Kelch domain containing 7B), Genebank Number (Genebank) BX647942 (Likely ortholog of mouse schlafen 5), Gene Registration Number (Genebank) AY358435 (Hypothetical protein MGC21688), Genebank BC004968 (Hypothetical protein MGC2664), Genebank AK125168 (Hypothetical protein MGC5356), Genebank NM_001620 [AHNAyokin nucleoprotein] desmo , Gene registration number (Genebank) NM_014632 (Microtubule associated monoxygenase, calponin and LIM domain containing 2), Gene registration number (Genebank) S77878, Gene registration number (Genebank) NM_003482 (Myeloid / lymphoid or mixed-lineage leukemia 2), Gene registration Number (Geneb ank) U38320 (Matrix metallopeptidase 19), Genebank NM_004530 [Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)], Genebank AJ298317 (Mucin 5, subtypes A and C, tracheobronchial / gastric), Genebank NM_002471 [Myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1)], Genebank (Genebank) AB209997 (Myosin IXB), Genebank (Genebank) NM_005967 NGFI-A binding protein 2 (EGR1 binding protein 2), Genebank AY358815 (Neural cell adhesion molecule 1), Genebank AB082526 [never in mitosis gene a) -related kinase 9 ], Genebank (Genebank) AK055224 (Neurogenic differentiation 6), Genebank (Genebank) NM_006162 (Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1), Genebank (AKene04) (Nuclear factor ( erythroid-derived 2) -like 1), gene registration number ( Genebank) AK074071 (Sperm equatorial segment protein 1), Genebank AK160377 (Nuclear pore complex interacting protein), Genebank NM_003873 (Neuropilin 1), Genebank AL833606 (Neuropilin 2), Genebank No. NM_001007156 (Neurotrophic tyrosine kinase, receptor, type 3), Genebank No. (Genebank) NM_015231 (Nucleoporin 160kDa), Genebank No. BC101462 (Neurexophilin 2), Gene Registration No. (Genebank) XM_371717 Odz, odd Oz / ten-m homolog 3 (Drosophila)], Genebank AI791380 [Transcribed locus, weakly similar to NP_079012. 2 gasdermin domain containing 1; 1810036L03Rik (Homo sapiens)], Genebank BC051352 (Oligodendrocyte transcription factor 3), Genebank (Genebank) NM_001004751 (Olfactory receptor, family 51, subfamily D, member 1), Genebank BG284742 [ P8 protein (candidate of metastasis 1)], Genebank NM_001618 [Poly (ADP-ribose) polymerase family, member 1], Genebank BC035485 [Poly (ADP-ribose) polymerase family, member 10 ], Genebank NM_032402 (Protocadherin gamma subfamily A, 12), Genebank XM_374898 [Pecanex-like 3 (Drosophila)], Genebank AK001872 (Programmed cell death 1 ligand 2) , Genebank AB209081 [Phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)], Genebank AB094096 (PDZ domain containing 2), Genebank AK127016 (PDZ domain containing 4), Genebank BX648284 [P elota homolog (Drosophila)], Genebank AF289554 (PERQ amino acid rich, with GYF domain 1), Genebank NM_006212 (6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 2 ), Genebank NM_003629 [Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma)], Genebank NM_013440 (Paired immunoglobin-like type 2 receptor beta), Genebank (Genebank) NM_052892 (Polycystic kidney disease 1-like 2), Genebank (Genebank) NM_201382 (Plectin 1, intermediate filament binding protein 500kDa), Genebank (Genebank) NM_032882 (Paraneoplastic antigen like 6A), Genebank (Genebank) NM_00093 [Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa], Genebank NM_000944 [(Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha)], Genebank (Genebank) ) BQ073453 (Protein kinase C, delta binding protein), gene registration number (Gene bank) NM_014502 [PRP19 / PSO4 pre-mRNA processing factor 19 homolog (S.  cerevisiae)], Genebank No. M23575 (Pregnancyspecific beta-1-glycoprotein 3), Genebank No. NM_002825 [Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)], Gene registration number ( Genebank) NM_002843 (Protein tyrosine phosphatase, receptor type, J), Genebank NM_002852 (Pentraxin-related gene, rapidly induced by IL-1 beta), Genebank BC017045 (Phosphorylase, glycogen; brain) , Gene Registration Number (Genebank) BM974642 (Transcribed locus), Gene Registration Number (Genebank) NM_002826 (Quiescin Q6), Gene Registration Number (Genebank) NM_181701 (Quiescin Q6-like 1), Gene Registration Number (Genebank) AF329499 (RAB27B, member RAS oncogene family, Genebank CR620019 (RAB43, member RAS oncogene family), Genebank NM_198679 [Rap guanine nucleotide exchange factor (GEF) 1], Genebank NM_002888 [Retinoic acid receptor responder (tazarotene induced) 1], Genebank AK092804 [Retinoic acid receptor responder (tazarotene induced) 2], Gene accession number (Genebank) X89399 (RAS p21 protein activator 3), Gene accession number (Genebank) NM_002891 (Ras protein-specific guanine nucleotide-releasing factor 1), genebank NG_002891 (Ras protein-specific guanine nucleotide-releasing factor 1), genebank AK128127 (Regulator of G-protein signaling 3), genebank (Genebank) BM980591 (Transcribed locus), Gene Registration Number (Genebank) BG564669 (Serum amyloid A2), Gene Registration Number (Genebank) NM_030754 (Serum amyloid A2), Gene Registration Number (Genebank) BG564326 (Serum amyloid A4, constitutive), Gene Registration Number (Genebank) BC022509 [Secretogranin II (chromogranin C)], Genebank NM_006514 (Sodium channel, voltage-gated, type X, alpha), Genebank NM_198056 [Sodium channel, voltage-gated, type V, alpha (long QT syndrome 3)], genes, etc. Number (Genebank) AB007937 [Syndecan 3 (N-syndecan)], gene registration number (Genebank) NM_002999 [Syndecan 4 (amphiglycan, ryudocan)], gene registration number (Genebank) AK122782 (Orphan short-chain dehydrogenase / reductase), gene Genebank NM_003004 (Secreted and transmembrane 1), Genebank NM_152754 [Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D], Genebank AK093049 [ Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3], Genebank AK058094 (Surfactant, pulmonary-associated protein C), Genebank NM_144579 (Sideroflexin 5), Gene registration Number (Genebank) AK074131 (SH3 multiple domains 4), Gene Registration Number (Genebank) BC098390 [Solute carrier family 12 (potassium / chloride transporters), member 7], Gene Registration Number (Genebank) AK091926 (Solute carrier family 13 (sodium-) dependent dicarboxylate transporter), member 2), heredity Genebank AK127216 (Solute carrier family 15, member 3), Genebank (Genebank) NM_004172 [Solutecarrier family 1 (glial high affinity glutamate transporter), member 3), Genebank (Menebank) NM_153320 [Solute carrier family 22 (organic anion transporter), member 7], Genebank NM_203327 [Solute carrier family 23 (nucleobase transporters), member 2], Genebank NM_152346 (Solute carrier family 43, member 2), Gene Genebank NM_201649 [Solute carrier family 6 (neurotransmitter transporter, glycine), member 9], Gene registration number (Genebank) NM_003045 [Solute carrier family 7 (cationic amino acid transporter, y + system), member 1], Gene registration Number (Genebank) NM_003486 [Solute carrier family 7 (cationic amino acid transporter, y + system), member 5], Gene accession number (Genebank) BC098388 [Slit homolog 3 (Drosophila)], Gene registration number (Genebank) NM_139067 (SWI / SNF related, matrix associated, actin depende nt regulator of chromatin, subfamily c, member 2), Genebank AK127286 (Spermine oxidase), Genebank NM_003955 (Suppressor of cytokine signaling 3), Genebank NM_003105 (Chromosome 11 open reading frame 32), Genebank NM_003106 [sex determining region Y] -box 2], Genebank NM_173360 (Spermatogenesis associated 9), Genebank BC017222 (Sequestosome 1) , Gene Registration Number (Genebank) NM_080836 (Serine / threonine kinase 35), Gene Registration Number (Genebank) AF545571 (Sulfatase 1), Gene Registration Number (Genebank) NM_177963 (Synaptotagmin XII), Gene Registration Number (Genebank) NM_000545 [Transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor], Genebank NM_000358 (Transforming growth factor, beta-induced, 68kDa), Genebank NM_015335 (Thyroid hormone receptor associated protein 2) , Genebank AB046767 [Transducin-like enhancer of split 3 (E (sp1) homolog, Drosophila)], Genebank (Genebank) AF367761 (Transmembrane protein 10), Genebank (Menebank) NM_002160 (Tenascin C) (hexabrachion), Genebank BC028148 [Tumor necrosis factor (TNF superfamily, member 2)], Genebank (Genebank) NM_006291 (Tumor necrosis factor, alpha-induced protein 2), Genebank (Genebank) NM_006290 (Tumor necrosis factor, alpha-induced protein 3), Genebank (Genebank) NM_007115 (Tumor necrosis factor, alpha-induced protein 6), Genebank (Genebank) BC052977 (Tumor necrosis factor receptor superfamily, member 1B), Genebank No. NM_003811 [Tumor necrosis factor (ligand) superfamily, member 9], Genebank AB210011 [Transportin 2 (importin 3, karyopherin beta 2b)], Genebank NM_005427 (Tumor protein p73), Genebank (Genebank) AB032995 (Two pore segment channel 1), Genebank S76980 [hexosaminidase A (alpha polypeptide)], Genebank (Genebank) NM_003320 [Tubby homolog (mouse)], Gene accession number (Genebank) NM_003328 (TXK tyrosine kinase, Genebank NM_006472 (Thioredoxin interacting protein), Gene Registration Number (Genebank) NM_003360 [UDP glycosyltransferase 8 (UDP-galactose ceramide galactosyltransferase)], Genebank NM_025154 [Unc-84 homolog A ( C.  elegans)], Genebank BC094797 [Unc-84 homolog B (C.  elegans)], Genebank AW016806 (Transcribed locus), Genebank A (Genebank) AW341701 (Transcribed locus), Genebank (Genebank) NM_199511 (Steroid sensitive gene 1), Genebank (Genebank) DQ097177 ( HECT, UBA and WWE domain containing 1), Genebank (Genebank) BC027954 (Tcell receptor gamma variable 7), Genebank (Genebank) NM_003371 (Vav 2 oncogene), Genebank (Genebank) AF167081 (Variable charge, X linked), Genebank AK058041 [Vestigial like 4 (Drosophila)], Genebank (Genebank) AJ132099 (Vanin 1), Genebank (Genebank) NM_014588 [Visual system homeobox 1 homolog, CHX10-like ( zebrafish)], Genebank NM_000638 [Vitronectin (serum spreading factor, somatomedin B, complement S-protein)], Genebank NM_022170 (Myosin XVB pseudogene), Genebank NM_032951 (MLX) interacting protein-like, Genebank BC 052234 (Wingless-type MMTV integration site family, member 10A), Genebank AK126706 (X-ray repair complementing defective repair in Chinese hamster cells 3), Genebank NM_020205 (Zinc finger, A20 domain containing 1), Genebank D26122 (splicing factor 1), Genebank NM_032441 (Zinc finger, matrin type 1), Genebank BC036823 (Zinc finger protein 289, ID1 regulated), gene Genebank NM_182975 (Zinc finger protein 326), Genebank NM_181489 (Zinc finger protein 445), Genebank BG201273 [Zinc finger protein 75 (D8C6)], Genebank (Genebank) AY008294 [Zinc finger protein, subfamily 1A, 2 (Helios)].

1) 상기 마커유전자 중에서 폐독성 유발 약물의 처리에 의하여 발현이 증가하는 마커유전자는 하기와 같다: 1) Among the marker genes, marker genes whose expression is increased by treatment of pulmonary toxicity-inducing drugs are as follows:

유전자등록번호(Genebank) BE378852(Transcribed locus), 유전자등록번호(Genebank)BG527520[Transcribed locus, moderately similar to XP_540962.1 PREDICTED: similar to centrin 4(Canis familiaris)]), 유전자등록번호(Genebank) NM_004797(Adiponectin, C1Q and collagen domain containing), 유전자등록번 호(Genebank) AF153821[Alcohol dehydrogenase IB(class I), beta polypeptide], 유전자등록번호(Genebank) NM_006818(Myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila); translocated to, 11), 유전자등록번호(Genebank) BP871540(Transcribed locus), 유전자등록번호(Genebank) AF269088(Ankyrin repeat domain 30B), 유전자등록번호(Genebank) BC018064(Activating transcription factor 6), 유전자등록번호(Genebank)XM_208270(Acrosin-like protease), 유전자등록번호(Genebank) NM_004323(BCL2-associated athanogene), 유전자등록번호(Genebank) BE816155(RC0-BN0198-220500-021-f04 BN0198 Homo sapiens cDNA, mRNA sequence.), 유전자등록번호(Genebank) NM_003670(Basic helix-loop-helix domain containing, class B, 2), 유전자등록번호(Genebank) BE614991[Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein(Pan troglodytes)], 유전자등록번호(Genebank) CN484301[Transcribed locus, moderately similar to XP_227769.2 PREDICTED: similar to Ac1147(Rattus norvegicus)], 유전자등록번호(Genebank) AK027152(Chromosome 11 open reading frame 1), 유전자등록번호(Genebank) XM_290629(Chromosome 14 open reading frame 78), 유전자등록번호(Genebank) NM_173817(Chromosome 18 open reading frame 58), 유전자등록번호(Genebank) CR625148(Hypothetical LOC541469 protein), 유전자등록번호(Genebank) BX537504(Complement component 2), 유전자등록번호(Genebank) BX281604(Chromosome 20 open reading frame 187), 유전자등록번호(Genebank) BU618438(Chromosome 20 open reading frame 77), 유전자등록번호(Genebank) BC020888(Metallophosphoesterase domain containing 1), 유전자등록번호(Genebank)NM_213720(Chromosome 22 open reading frame 16), 유전자등록번호(Genebank) BC027919(Chromosome 5 open reading frame 16), 유전자등록번호(Genebank) NM_030568(Chromosome 6 open reading frame 147), 유전자등록번호(Genebank) AK021476(Chromosome 6 open reading frame 164), 유전자등록번호(Genebank)BC036421(Chromosome 9 open reading frame 103), 유전자등록번호(Genebank) NM_153707(Chromosome 9 open reading frame 138), 유전자등록번호(Genebank) NM_203403(Chromosome 9 open reading frame 150), 유전자등록번호(Genebank) NM_021208(Chromosome 9 open reading frame 27), 유전자등록번호(Genebank) NM_053056(Cyclin D1), 유전자등록번호(Genebank) NM_001295[Chemokine(C-C motif) receptor 1], 유전자등록번호(Genebank) AK126349(CD200 receptor 1), 유전자등록번호(Genebank) AK026467(CD22 antigen), 유전자등록번호(Genebank) AK054921(Cerebellar degeneration-related protein 1, 34kDa), 유전자등록번호(Genebank) NM_012125(Cholinergic receptor, muscarinic 5), 유전자등록번호(Genebank) NM_003992(CDC-like kinase 3), 유전자등록번호(Genebank)A K022155(Coproporphyrinogen oxidase), 유전자등록번호(Genebank) AK090482[Chemokine(C-X-C motif) ligand 12(stromal cell-derived factor 1)], 유전자등록번호(Genebank) NM_152693(Chromosome X open reading frame 50), 유전자등록번호(Genebank) BX108307(Defensin, beta 126), 유전자등록번호(Genebank) AB051529[Dispatched homolog 2(Drosophila)], 유전자등록번호(Genebank) BC014951(chromosome 20 open reading frame 107), 유전자등록번호(Genebank) AK095541[DnaJ(Hsp40) homolog, subfamily A, member 1], 유전자등록번호(Genebank)BC036806(Extracellular matrix protein 2, female organ and adipocyte specific), 유전자등록번호(Genebank) BI830993(Eukaryotic translation initiation factor 4E), 유전자등록번호(Genebank) BX537939[Elastin(supravalvular aortic stenosis, Williams-Beuren syndrome)], 유전자등록번호(Genebank) AB051895(Epiplakin 1), 유전자등록번호(Genebank) AB209361[Fas(TNF receptor superfamily, member 6)], 유전자등록번호(Genebank) NM_033480(F-box protein 9), 유전자등록번호(Genebank)BC034763(Ferredoxin reductase), 유전자등록번호(Genebank) NM_005141(Fibrinogen beta chain), 유전자등록번호(Genebank) AL832445(Hypothetical protein FLJ25770), 유전자등록번호(Genebank) XM_499008(Hypothetical gene supported by AK124699), 유전자등록번호(Genebank) AK124499(RGS9 anchor protein), 유전자등록번호(Genebank) CR936597(Fibrous sheath interacting protein 2), 유전자등록번호(Genebank) NM_004293(Guanine deaminase), 유전자등록번호(Genebank) NM_177483(Glycosylphosphatidylinositol specific phospholipase D1), 유전자등록번호(Genebank) AK074729(G protein-coupled receptor 19), 유전자등록번호(Genebank) AK122626(G protein-coupled receptor 82), 유전자등록번호(Genebank) NM_014619(Glutamate receptor, ionotropic, kainate 4), 유전자등록 번호(Genebank) NM_002133[Heme oxygenase(decycling) 1], 유전자등록번호(Genebank)CB124304[Transcribed locus, strongly similar to NP_001604.1 alpha 2 actin; alpha-cardiac actin(Homo sapiens)], 유전자등록번호(Genebank) BE745738(CDNA clone IMAGE:3928921), 유전자등록번호(Genebank) BC043212(Hypothetical LOC401057), 유전자등록번호(Genebank) BC008624(Homo sapiens cDNA clone MGC:18299 IMAGE:4179890, complete cds.), 유전자등록번호(Genebank) AK024336(Homo sapiens cDNA FLJ14274 fis, clone PLACE1004979.), 유전자등록번호(Genebank) AK057952(CDNA FLJ25223 fis, clone STM00880), 유전자등록번호(Genebank) AK123483(CDNA FLJ41489 fis, clone BRTHA2004582), 유전자등록번호(Genebank) BU570278[Similar to dJ1153D9.4(novel protein)], 유전자등록번호(Genebank) BE614991[Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein(Pan troglodytes)], 유전자등록번호(Genebank) T77197(TBC1 domain family, member 3B), 유전자등록번호(Genebank) AF119839(predicted protein of HQ0889; Homo sapiens PRO0889 mRNA, complete cds.), 유전자등록번호(Genebank) AF119912(predicted protein of HQ3073; Homo sapiens PRO3073 mRNA, complete cds.), 유전자등록번호(Genebank) BM467031(Ribosomal protein S2), 유전자등록번호(Genebank) CA943742[Transcribed locus, strongly similar to XP_429266.1 PREDICTED: hypothetical protein XP_429266(Gallus gallus)], 유전자등록번호(Genebank) AV727525(Transcribed locus), 유전자등록번호(Genebank) BC015351(Homo sapiens, clone IMAGE:4420333, mRNA), 유전자등록번호(Genebank) BG759321(Homo sapiens, clone IMAGE:4852110, mRNA), 유전자등록번호(Genebank) BF337308(Homeodomain-only protein), 유전자등록번호(Genebank) AK223249(Homeo box B7), 유전자등록번호(Genebank) NM_021192(Homeo box D11), 유전자등록번호(Genebank) NM_005345(Heat shock 70kDa protein 1A), 유전자등록번호(Genebank) NM_006597(Heat shock 70kDa protein 8), 유전자등록번호(Genebank) U88896, 유전자등록번호(Genebank) NM_134470(Interleukin 1 receptor accessory protein), 유전자등록번호(Genebank) BC069137[Interleukin 5(colony-stimulating factor, eosinophil)], 유전자등록번호(Genebank) AB039920(KCNQ1 downstream neighbor), 유전자등록번호(Genebank) AB033055(Outer dense fiber of sperm tails 2-like), 유전자등록번호(Genebank) AK001632(Hypothetical protein FLJ10770), 유전자등록번호(Genebank) BC042437[Keratin 14(epidermolysis bullosa simplex, Dowling-Meara, Koebner)], 유전자등록번호(Genebank) BX647923(Keratin 17), 유전자등록번호(Genebank) BC025396[Spermatogenesis associated 18 homolog(rat)], 유전자등록번호(Genebank) BC016613(Similar to hypothetical protein FLJ13841), 유전자등록번호(Genebank) BU536672(Ribosomal protein L22-like 1), 유전자등록번호(Genebank) BC012203(Hypothetical protein LOC284361), 유전자등록번호(Genebank) BC044311(Hypothetical protein LOC339344), 유전자등록번호(Genebank) AK022806(Hypothetical protein LOC340281), 유전자등록번호(Genebank) XM_371643(Similar to hypothetical protein MGC39725), 유전자등록번호(Genebank) XM_372625(similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author), 유전자등록번호(Genebank) XM_498623(LOC440309), 유전자등록번호(Genebank) XM_496818(similar to RIKEN cDNA 2410146L05), 유전자등록번호(Genebank) XM_497458(Similar to large subunit ribosomal protein L36a), 유전자등록번호(Genebank) AK127395(Ankyrin repeat domain 37), 유전자등록번호(Genebank) AK126961(Leucine rich repeat transmembrane neuronal 4), 유전자등록번호(Genebank) AK026984(Multiple C2 domains, transmembrane 2), 유전자등록번호(Genebank) BE644770(Mitofusin 1), 유전자등록번호(Genebank) AK123160(Chromosome 1 open reading frame 162), 유전자등록번호(Genebank) AK125877(Hypothetical protein MGC27016), 유전자등록번호(Genebank) AK124107(Leucine rich repeat containing 43), 유전자등록번호(Genebank) AK097334(Hypothetical protein MGC45840), 유전자등록번호(Genebank) AK123482(Microsomal glutathione S-transferase 1), 유전자등록번호(Genebank) AK128769(calcium channel, voltage-dependent, beta 2 subunit), 유전자등록번호(Genebank) AY154467(NACHT, leucine rich repeat and PYD containing 12), 유전자등록번호(Genebank) AK124709(N-myc downstream regulated gene 1), 유전자등록번호(Genebank) NM_016931(NADPH oxidase 4), 유전자등록번호(Genebank) BX640730(Nebulin-related anchoring protein), 유전자등록번호(Genebank) AA524504(Full length insert cDNA clone ZE12C10), 유전자등록번호(Genebank)NM_002563(Purinergic receptor P2Y, G-protein coupled, 1), 유전자 등록번호(Genebank) AL834134(Protocadherin 15), 유전자등록번호(Genebank) NM_001011515(PDZ and LIM domain 5), 유전자등록번호(Genebank) S81916(Phosphoglycerate kinase 1), 유전자등록번호(Genebank) NM_003662[Pirin(iron-binding nuclear protein)], 유전자등록번호(Genebank) NM_033256[Protein phosphatase 1, regulatory(inhibitor) subunit 14A], 유전자등록번호(Genebank) NM_014068(Psoriasis susceptibility 1 candidate 1), 유전자등록번호(Genebank) NM_002900(Retinol binding protein 3, interstitial), 유전자등록번호(Genebank) AK055999(Ret finger protein-like 2), 유전자등록번호(Genebank) AB209019(Regulator of G-protein signalling 4), 유전자등록번호(Genebank) NM_004040(Ras homolog gene family, member B), 유전자등록번호(Genebank) BU902070(Ribosomal protein L36a-like), 유전자등록번호(Genebank) BF983391(Ribosomal protein S27-like), 유전자등록번호(Genebank) NM_173640[R-spondin homolog(Xenopus laevis)], 유전자등록번호(Genebank) BG182941[Transcribed locus, weakly similar to XP_510104.1 PREDICTED: similar to hypothetical protein FLJ25224(Pan troglodytes)], 유전자등록번호(Genebank) NM_004057(S100 calcium binding protein G), 유전자등록번호(Genebank) BC041022(SCAN domain containing 1), 유전자등록번호(Genebank) NM_002639[Serpin peptidase inhibitor, clade B(ovalbumin), member 5], 유전자등록번호(Genebank) NM_005668(ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4), 유전자등록번호(Genebank) NM_003830(Sialic acid binding Ig-like lectin 5), 유전자 등록번호(Genebank) BC053934[Sirtuin(silent mating type information regulation 2 homolog) 4(S. cerevisiae)], 유전자등록번호(Genebank) NM_052832(Solute carrier family 26, member 7), 유전자등록번호(Genebank) NM_014332(Small muscle protein, X-linked), 유전자등록번호(Genebank) BC026316[Suppression of tumorigenicity 18(breast carcinoma)(zinc finger protein)], 유전자등록번호(Genebank) AK122707(Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3), 유전자등록번호(Genebank) AK026585(Tumor-associated calcium signal transducer 1), 유전자등록번호(Genebank) AL038125(Zinc finger, AN1-type domain 3), 유전자등록번호(Genebank) NM_000361(Thrombomodulin), 유전자등록번호(Genebank) BC010553(TIMP metallopeptidase inhibitor 4), 유전자등록번호(Genebank) NM_003840(Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), 유전자등록번호(Genebank) NM_004881(Tumor protein p53 inducible protein 3), 유전자등록번호(Genebank) NM_033229(Tripartite motif-containing 15), 유전자등록번호(Genebank) BC063872(Tripartite motif-containing 9), 유전자등록번호(Genebank) NM_003314(Tetratricopeptide repeat domain 1), 유전자등록번호(Genebank) AK024002(Tubulin, alpha 4), 유전자등록번호(Genebank) NM_006087(Tubulin, beta 4), 유전자등록번호(Genebank) AF219946(Tubby like protein 4), 유전자등록번호(Genebank) AK025742[Uncoupling protein 2(mitochondrial, proton carrier)], 유전자등록번호(Genebank) AI699331(Transcribed locus), 유전자등록번 호(Genebank) AF055634[Unc-5 homolog C(C. elegans)], 유전자등록번호(Genebank) AK023940(Homo sapiens cDNA FLJ13878 fis, clone THYRO1001411), 유전자등록번호(Genebank) BE815283(Transcribed locus), 유전자등록번호(Genebank) BM913228[Full-length cDNA clone CS0DI020YI20 of Placenta Cot 25-normalized of Homo sapiens(human)], 유전자등록번호(Genebank) BX099682(Transcribed locus), 유전자등록번호(Genebank) BX640772[MRNA; cDNA DKFZp686P02129(from clone DKFZp686P02129)], 유전자등록번호(Genebank) AK094191(Chromosome 8 open reading frame 34), 유전자등록번호(Genebank) NM_033400(Zinc finger homeobox 2), 유전자등록번호(Genebank) XM_040592(Zinc finger protein 469).Genebank BE378852 (Transcribed locus), Genebank BG527520 [Transcribed locus, moderately similar to XP_540962.1 PREDICTED: similar to centrin 4 (Canis familiaris)], Genebank NM_004797 ( Adiponectin, C1Q and collagen domain containing), Genebank AF153821 [Alcohol dehydrogenase IB (class I), beta polypeptide], Genebank NM_006818 (Myeloid / lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 11), Genebank BP871540 (Transcribed locus), Genebank AF269088 (Ankyrin repeat domain 30B), Genebank BC018064 (Activating transcription factor 6), Gene Genebank XM_208270 (Acrosin-like protease), Genebank (Genebank) NM_004323 (BCL2-associated athanogene), Genebank (Genebank) BE816155 (RC0-BN0198-220500-021-f04 BN0198 Homo sapiens cDNA, mRNA sequence.), genes, etc. Genebank NM_003670 (Basic helix-loop-helix domain containing, class B, 2), Genebank BE614991 [Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein (Pan troglodytes)], Genebank CN484301 [Transcribed locus, moderately similar to XP_227769.2 PREDICTED: similar to Ac1147 (Rattus norvegicus)], Genebank AK027152 (Chromosome 11 open reading frame 1), Genebank (Genebank) XM_290629 (Chromosome 14 open reading frame 78), Genebank NM_173817 (Chromosome 18 open reading frame 58), Genebank (Genebank) CR625148 (Hypothetical LOC541469 protein), Genebank (Genebank) BX537504 (Complement component 2 Genebank BX281604 (Chromosome 20 open reading frame 187), Genebank BU618438 (Chromosome 20 open reading frame 77), Genebank BC020888 (Metallophosphoesterase domain containin) g 1), Genebank NM_213720 (Chromosome 22 open reading frame 16), Genebank number (Genebank) BC027919 (Chromosome 5 open reading frame 16), Gene accession number (Genebank) NM_030568 (Chromosome 6 open reading frame 147 ), Genebank AK021476 (Chromosome 6 open reading frame 164), Gene Registration Number (Genebank) BC036421 (Chromosome 9 open reading frame 103), Gene Registration Number (Genebank) NM_153707 (Chromosome 9 open reading frame 138), Genebank No. NM_203403 (Chromosome 9 open reading frame 150), Genebank No. NM_021208 (Chromosome 9 open reading frame 27), Genebank No. NM_053056 (Cyclin D1), Genebank No. (Genebank) NM_001295 [Chemokine (CC motif) receptor 1], Genebank AK126349 (CD200 receptor 1), Genebank AK026467 (CD22 antigen), Genebank AK054921 (Cerebellar degeneration-related protein 1 , 34kDa), Gene Registration Number (G enebank) NM_012125 (Cholinergic receptor, muscarinic 5), gene accession number (Genebank) NM_003992 (CDC-like kinase 3), gene accession number (Genebank) A K022155 (Coproporphyrinogen oxidase), gene accession number (Genebank) AK090482 [Chemokine (CXC) motif ligand 12 (stromal cell-derived factor 1)], Genebank NM_152693 (Chromosome X open reading frame 50), Genebank BX108307 (Defensin, beta 126), Genebank (Genebank) AB051529 [Dispatched homolog 2 (Drosophila)], Genebank BC014951 (chromosome 20 open reading frame 107), Genebank AK095541 [DnaJ (Hsp40) homolog, subfamily A, member 1], Gene accession number (Genebank) BC036806 (Extracellular matrix protein 2, female organ and adipocyte specific), Genebank BI830993 (Eukaryotic translation initiation factor 4E), Genebank BX537939 [Elastin (supravalvular aortic stenosis, Williams-Beuren syndrome) )], Gene Registration Genebank AB051895 (Epiplakin 1), Genebank AB209361 [Fas (TNF receptor superfamily, member 6)], Genebank NM_033480 (F-box protein 9), Gene Registration Number (Genebank) BC034763 (Ferredoxin reductase), Genebank NM_005141 (Fibrinogen Beta Chain), Genebank AL832445 (Hypothetical protein FLJ25770), Genebank XM_499008 (Hypothetical gene supported by AK124699), Gene Registration Number (Genebank) AK124499 (RGS9 anchor protein), Genebank CR936597 (Fibrous sheath interacting protein 2), Genebank NM_004293 (Guanine deaminase), Genebank NM_177483 (Glycosylphosphatidylinositol specific phospholipase D) , Genebank AK074729 (G protein-coupled receptor 19), Genebank AK122626 (G protein-coupled receptor 82), Genebank NM_014619 (Glutamate receptor, ionotropic, kainate 4), Genebank NM_002133 [Heme oxygenase (decycling 1), Genebank CB124304 [Transcribed locus, strongly similar to NP_001604.1 alpha 2 actin; alpha-cardiac actin (Homo sapiens)], Genebank BE745738 (CDNA clone IMAGE: 3928921), Gene Registration Number (Genebank) BC043212 (Hypothetical LOC401057), Gene Registration Number (Genebank) BC008624 (Homo sapiens cDNA clone MGC : 18299 IMAGE: 4179890, complete cds.), Genebank Number (Genebank) AK024336 (Homo sapiens cDNA FLJ14274 fis, clone PLACE1004979.), Genebank Number (Genebank) AK057952 (CDNA FLJ25223 fis, clone STM00880), Gene Registration Number (Genebank) Genebank) AK123483 (CDNA FLJ41489 fis, clone BRTHA2004582), Genebank BU570278 [Similar to dJ1153D9.4 (novel protein)], Genebank BE614991 [Transcribed locus, strongly similar to XP_514491.1 PREDICTED: similar to hypothetical protein (Pan troglodytes)], Genebank T77197 (TBC1 domain family, member 3B), Genebank AF119839 (predicted protein of HQ0889; Homo sapiens PRO0889 mRNA, complete cds.), gene Registration Number (Genebank) AF119912 (predicted pr otein of HQ3073; Homo sapiens PRO3073 mRNA, complete cds.), Genebank BM467031 (Ribosomal protein S2), Genebank CA943742 [Transcribed locus, strongly similar to XP_429266.1 PREDICTED: hypothetical protein XP_429266 ( Gallus gallus)], Genebank AV727525 (Transcribed locus), Genebank (Genebank) BC015351 (Homo sapiens, clone IMAGE: 4420333, mRNA), Genebank BG759321 (Homo sapiens, clone IMAGE: 4852110, mRNA), Genebank (Genebank) BF337308 (Homeodomain-only protein), Genebank (Genebank) AK223249 (Homeo box B7), Genebank (Genebank) NM_021192 (Homeo box D11), Genebank (Genebank) ) NM_005345 (Heat shock 70kDa protein 1A), Genebank Number (Genebank) NM_006597 (Heat shock 70kDa Protein 8), Gene Registration Number (Genebank) U88896, Genebank Number (Genebank) NM_134470 (Interleukin 1 receptor accessory protein), Gene Registration Number (Genebank) BC069137 [Interl eukin 5 (colony-stimulating factor, eosinophil)], Genebank AB039920 (KCNQ1 downstream neighbor), Genebank AB033055 (Outer dense fiber of sperm tails 2-like), Genebank (Genebank) AK001632 (Hypothetical protein FLJ10770), Genebank BC042437 [Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)], Genebank BX647923 (Keratin 17), Genebank BC025396 [ Spermatogenesis associated 18 homolog (rat)], Genebank BC016613 (Similar to hypothetical protein FLJ13841), Genebank BU536672 (Ribosomal protein L22-like 1), Genebank BC012203 (Hypothetical protein LOC284361), Genebank BC044311 (Hypothetical protein LOC339344), Genebank AK022806 (Hypothetical protein LOC340281), Genebank XM_371643 (Similar to hypothetical protein MGC39725), Genes, etc. Number (Genebank) XM_372625 (similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author, Genebank XM_498623 (LOC440309), Genebank XM_496818 (similar to RIKEN cDNA 2410146L05), Genebank XM_497458 (Similar to large subunit ribosomal protein L36a ), Genebank AK127395 (Ankyrin repeat domain 37), Genebank AK126961 (Leucine rich repeat transmembrane neuronal 4), Genebank AK026984 (Multiple C2 domains, transmembrane 2), Gene registration Genebank BE644770 (Mitofusin 1), Genebank AK123160 (Chromosome 1 open reading frame 162), Genebank AK125877 (Hypothetical protein MGC27016), Genebank AK124107 (Leucine rich repeat containing 43), Genebank AK097334 (Hypothetical protein MGC45840), Genebank AK123482 (Microsomal glutathione S-transferase 1), Genebank AK128769 (calcium c) hannel, voltage-dependent, beta 2 subunit), Genebank AY154467 (NACHT, leucine rich repeat and PYD containing 12), Genebank AK124709 (N-myc downstream regulated gene 1), Gene Registration Number (Genebank) NM_016931 (NADPH oxidase 4), Genebank BX640730 (Nebulin-related anchoring protein), Genebank AA524504 (Full length insert cDNA clone ZE12C10), Genebank NM_002563 (Purinergic receptor P2Y, G-protein coupled, 1), gene registration number (Genebank) AL834134 (Protocadherin 15), gene registration number (Genebank) NM_001011515 (PDZ and LIM domain 5), gene registration number (Genebank) S81916 (Phosphoglycerate kinase 1) , Genebank NM_003662 [Pirin (iron-binding nuclear protein)], Genebank (Genebank) NM_033256 [Protein phosphatase 1, regulatory (inhibitor) subunit 14A], Genebank (Menebank) NM_014068 (Psoriasis susceptibility 1 candidate 1), Gene registration number (Genebank) NM_002900 (Retinol binding protein 3, interstitial), Genebank AK055999 (Ret finger protein-like 2), Genebank AB209019 (Regulator of G-protein signaling 4), Gene registration number ( Genebank) NM_004040 (Ras homolog gene family, member B), Genebank BU902070 (Ribosomal protein L36a-like), Genebank BF983391 (Ribosomal protein S27-like), Gene accession number (Genebank) NM_173640 [R-spondin homolog (Xenopus laevis)], Genebank BG182941 [Transcribed locus, weakly similar to XP_510104.1 PREDICTED: similar to hypothetical protein FLJ25224 (Pan troglodytes)], Genebank NM_004057 (S100 calcium binding protein G), Genebank BC041022 (SCAN domain containing 1), Genebank NM_002639 [Serpin peptidase inhibitor, clade B (ovalbumin), member 5], Genebank NM_005668 ( ST8 alpha-N-acetyl-neuraminide alpha-2,8-sia lyltransferase 4), Genebank No. NM_003830 (Sialic acid binding Ig-like lectin 5), Gene Registration No. (Genebank) BC053934 [Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)], Genebank NM_052832 (Solute carrier family 26, member 7), Genebank NM_014332 (Small muscle protein, X-linked), Genebank BC026316 [Suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein)], Genebank AK122707 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3), Genebank AK026585 (Tumor-associated calcium signal transducer 1) , Genebank AL038125 (Zinc finger, AN1-type domain 3), Genebank (Genebank) NM_000361 (Thrombomodulin), Genebank (Genebank) BC010553 (TIMP metallopeptidase inhibitor 4), Genebank (Genebank) NM_003840 (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), Genebank NM_004881 (Tumor protein p53 inducible protein 3), Genebank NM_033229 (Tripartite motif-containing 15), gene Enrollment Genebank BC063872 (Tripartite motif-containing 9), Genebank NM_003314 (Tetrictricopeptide repeat domain 1), Genebank AK024002 (Tubulin, alpha 4), Genebank NM_006087 (Tubulin , beta 4), Genebank AF219946 (Tubby like protein 4), Genebank AK025742 [Uncoupling protein 2 (mitochondrial, proton carrier)], Genebank AI699331 (Transcribed locus), Genebank AF055634 [Unc-5 homolog C (C. elegans)], Genebank AK023940 (Homo sapiens cDNA FLJ13878 fis, clone THYRO1001411), Genebank BE815283 (Transcribed locus), Genebank BM913228 [Full-length cDNA clone CS0DItaYI20 of Placen Cot 25-normalized of Homo sapiens (human)], Genebank BX099682 (Transcribed locus), Genebank BX640772 [MRNA; cDNA DKFZp686P02129 (from clone DKFZp686P02129), Genebank AK094191 (Chromosome 8 open reading frame 34), Genebank NM_033400 (Zinc finger homeobox 2), Genebank XM_040592 (Zinc finger protein) 469).

2) 상기 마커유전자 중에서, 폐독성 유발 약물의 처리에 의하여 발현이 감소하는 마커유전자는 하기와 같다: 2) Among the marker genes, marker genes whose expression is reduced by treatment of pulmonary toxicity-inducing drugs are as follows:

유전자등록번호(Genebank)(Genebank) NM_007313(V-abl Abelson murine leukemia viral oncogene homolog 1), 유전자등록번호(Genebank) NM_203380(Acyl-CoA synthetase long-chain family member 5), 유전자등록번호(Genebank) BC040531(Activin A receptor, type IB), 유전자등록번호(Genebank) Y13786[ADAM metallopeptidase domain 19(meltrin beta)], 유전자등록번호(Genebank) AJ250725(ADAM metallopeptidase with thrombospondin type 1 motif, 12), 유전자등록번호(Genebank) AJ315733(ADAM metallopeptidase with thrombospondin type 1 motif, 15), 유전자등록번호(Genebank) NM_133638(ADAM metallopeptidase with thrombospondin type 1 motif, 19), 유전자등록번호(Genebank) NM_014243(ADAM metallopeptidase with thrombospondin type 1 motif, 3), 유전자등록번호(Genebank) NM_207517(ADAMTS-like 3), 유전자등록번호(Genebank) BC013780(Adenosine A2a receptor), 유전자등록번호(Genebank) AK160382(AT-hook transcription factor), 유전자등록번호(Genebank) AK122839(V-akt murine thymoma viral oncogene homolog 2), 유전자등록번호(Genebank) AK128709(Aldehyde dehydrogenase 1 family, member A2), 유전자등록번호(Genebank) NM_003748(Aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(Genebank) NM_172196(TFIIA-alpha/beta-like factor), 유전자등록번호(Genebank) NM_152526[Amyotrophic lateral sclerosis 2(juvenile) chromosome region, candidate 19], 유전자등록번호(Genebank) NM_139314(Angiopoietin-like 4), 유전자등록번호(Genebank) NM_000037(Ankyrin 1, erythrocytic), 유전자등록번호(Genebank) AJ583444[Ankyrin repeat domain 2(stretch responsive muscle)], 유전자등록번호(Genebank) NM_145641(Apolipoprotein L, 3), 유전자등록번호(Genebank) BC006276(Apolipoprotein L, 4), 유전자등록번호(Genebank) NM_003224(ADP-ribosylation factor related protein 1), 유전자등록번호(Genebank) NM_021069(Sorbin and SH3 domain containing 2), 유전자등록번호(Genebank) AK090447(Rho GTPase activating protein 19), 유전자등록번호(Genebank) NM_015071(Rho GTPase activating protein 26), 유전자등록번 호(Genebank) NM_032199[AT rich interactive domain 5B(MRF1-like)], 유전자등록번호(Genebank) NM_183376(Arrestin domain containing 4), 유전자등록번호(Genebank) AK000643(Ankyrin repeat and SOCS box-containing 9), 유전자등록번호(Genebank) AB209032(Activating transcription factor 3), 유전자등록번호(Genebank) NM_032827[Atonal homolog 8(Drosophila)], 유전자등록번호(Genebank) BX537444(ATPase, Ca++ transporting, plasma membrane 4), 유전자등록번호(Genebank) NM_006045(ATPase, Class II, type 9A), 유전자등록번호(Genebank) AB011106(Attractin-like 1), 유전자등록번호(Genebank) NM_000707(Arginine vasopressin receptor 1B), 유전자등록번호(Genebank) CR933657[Axin 2(conductin, axil)], 유전자등록번호(Genebank) NM_152384(Bardet-Biedl syndrome 5), 유전자등록번호(Genebank) NM_021948(Brevican), 유전자등록번호(Genebank) AA608624(Breast carcinoma amplified sequence 3), 유전자등록번호(Genebank) NM_017745(BCL6 co-repressor), 유전자등록번호(Genebank) S72478, 유전자등록번호(Genebank) NM_001710(B-factor, properdin), 유전자등록번호(Genebank) CR749373(chromosome 1 open reading frame 178), 유전자등록번호(Genebank) AL713697(Bridging integrator 1), 유전자등록번호(Genebank) NM_001165(Baculoviral IAP repeat-containing 3), 유전자등록번호(Genebank) BM984396(Plasminogen-like B2), 유전자등록번호(Genebank) NM_001203(Bone morphogenetic protein receptor, type IB), 유전자등록번호(Genebank) AK098095(V-raf murine sarcoma viral oncogene homolog B1), 유전자등록번 호(Genebank) NM_014299(Bromodomain containing 4), 유전자등록번호(Genebank) BC016743[BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB(S. cerevisiae)], 유전자등록번호(Genebank) NM_001728[Basigin(OK blood group)], 유전자등록번호(Genebank) NM_052876[BTB(POZ) domain containing 14B], 유전자등록번호(Genebank) BX537534[BTB(POZ) domain containing 9], 유전자등록번호(Genebank) BX107768(Transcribed locus), 유전자등록번호(Genebank) AY665468[Ras association(RalGDS/AF-6) domain family 8], 유전자등록번호(Genebank) NM_033513(Chromosome 19 open reading frame 20), 유전자등록번호(Genebank) BC068439(Chromosome 19 open reading frame 28), 유전자등록번호(Genebank) AY358424(C1q and tumor necrosis factor related protein 1), 유전자등록번호(Genebank) NM_001733(Complement component 1, r subcomponent), 유전자등록번호(Genebank) NM_080834(Chromosome 20 open reading frame 152), 유전자등록번호(Genebank) AK000652(Chromosome 20 open reading frame 57), 유전자등록번호(Genebank) BC063852(Complement component 3), 유전자등록번호(Genebank) AF251038(Chromosome 5 open reading frame 5), 유전자등록번호(Genebank)B X640641(Chromosome 6 open reading frame 107), 유전자등록번호(Genebank) AL832416(Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1), 유전자등록번호(Genebank) BC021552(Calcium channel, voltage-dependent, gamma subunit 6), 유전자등록번호(Genebank) AL117636(Calneuron 1), 유전자등록번호(Genebank) AJ519841(Calmodulin regulated spectrin-associated protein 1), 유전자등록번호(Genebank) AK128124(Calpain 5), 유전자등록번호(Genebank) NM_002982[Chemokine(C-C motif) ligand 2], 유전자등록번호(Genebank) BC020698[Chemokine(C-C motif) ligand 20], 유전자등록번호(Genebank) BC071718[Chemokine(C-C motif) ligand 4-like 1], 유전자등록번호(Genebank) CR614015(CD14 antigen), 유전자등록번호(Genebank) NM_001004196(CD200 antigen), 유전자등록번호(Genebank) AK090470[CD33 antigen(gp67)], 유전자등록번호(Genebank) NM_001773(CD34 antigen), 유전자등록번호(Genebank) BC025729(CD99 antigen-like 2), 유전자등록번호(Genebank) AB051503(Centaurin, beta 5), 유전자등록번호(Genebank) NM_032221(Chromodomain helicase DNA binding protein 6), 유전자등록번호(Genebank) NM_000740(Cholinergic receptor, muscarinic 3), 유전자등록번호(Genebank) NM_016369(Claudin 18), 유전자등록번호(Genebank) AB007969[Calmin(calponin-like, transmembrane)], 유전자등록번호(Genebank) AJ278018(Calsyntenin 2), 유전자등록번호(Genebank)AJ626900(Cardiomyopathy associated 1), 유전자등록번호(Genebank) NM_020872[Contactin 3(plasmacytoma associated)], 유전자등록번호(Genebank) NM_004370(Collagen, type XII, alpha 1), 유전자등록번호(Genebank) Z74615(Collagen, type I, alpha 1), 유전자등록번호(Genebank) NM_001845(Collagen, type IV, alpha 1), 유전자등록번호(Genebank) NM_033380[Collagen, type IV, alpha 5(Alport syndrome)], 유전자등록번호(Genebank) NM_000393(Collagen, type V, alpha 2), 유전자등록번호(Genebank) NM_001848(Collagen, type VI, alpha 1), 유전자등록번호(Genebank) NM_001850(Collagen, type VIII, alpha 1), 유전자등록번호(Genebank) AK124894(Corticotropin releasing hormone receptor 1), 유전자등록번호(Genebank) BC021117[Colony stimulating factor 1(macrophage)], 유전자등록번호(Genebank) BE873976[Colony stimulating factor 2(granulocyte-macrophage)], 유전자등록번호(Genebank) NM_172220[Colony stimulating factor 3(granulocyte)], 유전자등록번호(Genebank) AK092391(Cystatin E/M), 유전자등록번호(Genebank) NM_147780(Cathepsin B), 유전자등록번호(Genebank) NM_001334(Cathepsin O), 유전자등록번호(Genebank)NM_198700(CUG triplet repeat, RNA binding protein 1), 유전자등록번호(Genebank)NM_015267[Cut-like 2(Drosophila)], 유전자등록번호(Genebank) NM_001511[Chemokine(C-X-C motif) ligand 1(melanoma growth stimulating activity, alpha)], 유전자등록번호(Genebank)BC053653[Chemokine(C-X-C motif) ligand 2], 유전자등록번호(Genebank) NM_002090[Chemokine(C-X-C motif) ligand 3], 유전자등록번호(Genebank) NM_002994[Chemokine(C-X-C motif) ligand 5], 유전자등록번호(Genebank) U81234[Chemokine(C-X-C motif) ligand 6(granulocyte chemotactic protein 2)], 유전자등록번호(Genebank) NM_000497(Cytochrome P450, family 11, subfamily B, polypeptide 1), 유전자등록번호(Genebank) NM_004393[Dystroglycan 1(dystrophin-associated glycoprotein 1)], 유전자등록번호(Genebank) BX537651(Discoidin domain receptor family, member 2), 유전자등록번호(Genebank) AB209635(Diacylglycerol kinase, zeta 104kDa), 유전자등록번호(Genebank) AB209653[Dehydrogenase/reductase(SDR family) member 2], 유전자등록번호(Genebank) NM_004753[Dehydrogenase/reductase(SDR family) member 3], 유전자등록번호(Genebank) AL832637(Taxilin alpha), 유전자등록번호(Genebank) BX640676(Regeneration associated muscle protease), 유전자등록번호(Genebank) XM_291222(Integrator complex subunit 1), 유전자등록번호(Genebank) NM_031294(Leucine rich repeat containing 48), 유전자등록번호(Genebank)NM_182643(Deleted in liver cancer 1), 유전자등록번호(Genebank) NM_001365[Discs, large homolog 4(Drosophila)], 유전자등록번호(Genebank) NM_007329(Deleted in malignant brain tumors 1), 유전자등록번호(Genebank) BM808864(Dopamine receptor D1 interacting protein), 유전자등록번호(Genebank) AK057845(Ephrin-A1), 유전자등록번호(Genebank) NM_012199(Eukaryotic translation initiation factor 2C, 1), 유전자등록번호(Genebank) BX537368[E74-like factor 3(ets domain transcription factor, epithelial-specific)], 유전자등록번호(Genebank) NM_001426(Engrailed homolog 1), 유전자등록번호(Genebank) BC054508(Erythrocyte membrane protein band 4.1 like 5), 유전자등록번호(Genebank) AF073727(Epsin 1), 유전자등록번호(Genebank) BC060883(Epsin 2), 유전자등록번호(Genebank) AL831953[Epithelial stromal interaction 1(breast)], 유전자등록번호(Genebank) D79987[Extra spindle poles like 1(S. cerevisiae)], 유전자등록번호(Genebank) NM_00198[Ets variant gene 6(TEL oncogene)], 유전자등 록번호(Genebank) BX648769(Ewing sarcoma breakpoint region 1), 유전자등록번호(Genebank) NM_014661(Family with sequence similarity 53, member B), 유전자등록번호(Genebank) NM_001004019(Fibulin 2), 유전자등록번호(Genebank) AK125888(F-box protein 32), 유전자등록번호(Genebank) NM_002016(Filaggrin), 유전자등록번호(Genebank) NM_024669(FLJ11795 protein), 유전자등록번호(Genebank) NM_024817(Hypothetical protein FLJ13710), 유전자등록번호(Genebank) NM_017631(Hypothetical protein FLJ20035), 유전자등록번호(Genebank) BQ279256(Hypothetical protein FLJ20294), 유전자등록번호(Genebank) AK123641(Cleavage and polyadenylation specific factor 3-like), 유전자등록번호(Genebank) AK024488(Hypothetical protein FLJ21438), 유전자등록번호(Genebank) AL832659(Hypothetical protein FLJ22833), 유전자등록번호(Genebank) NM_181645(Hypothetical protein FLJ25393), 유전자등록번호(Genebank) AL833626(Zinc finger, DHHC-type containing 20), 유전자등록번호(Genebank) NM_173621(Chromosome 17 open reading frame 44), 유전자등록번호(Genebank) NM_001460(Flavin containing monooxygenase 2), 유전자등록번호(Genebank) NM_001461(Flavin containing monooxygenase 5), 유전자등록번호(Genebank) NM_212482(Fibronectin 1), 유전자등록번호(Genebank) AB033068[Fizzy/cell division cycle 20 related 1(Drosophila)], 유전자등록번호(Genebank) NM_080612(GRB2-associated binding protein 3), 유전자등록번호(Genebank) BC051463[Gamma-aminobutyric acid(GABA) B receptor, 1], 유전자등 록번호(Genebank) NM_198321[UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10(GalNAc-T10)], 유전자등록번호(Genebank) NM_198335(Glucosidase, alpha; neutral AB), 유전자등록번호(Genebank) NM_002048(Growth arrest-specific 1), 유전자등록번호(Genebank)AK127845(GATA binding protein 2), 유전자등록번호(Genebank) NM_000514(Glial cell derived neurotrophic factor), 유전자등록번호(Genebank) NM_000165[Gap junction protein, alpha 1, 43kDa(connexin 43)], 유전자등록번호(Genebank) BC043390(Glucagon-like peptide 2 receptor), 유전자등록번호(Genebank) AF288389(Glycosyltransferase 25 domain containing 2), 유전자등록번호(Genebank) BC051726[Glutamate-ammonia ligase(glutamine synthetase)], 유전자등록번호(Genebank) CR936770[Guanine nucleotide binding protein(G protein), alpha activating activity polypeptide O], 유전자등록번호(Genebank)BC095544(G protein-coupled receptor 32), 유전자등록번호(Genebank) NM_000841(Glutamate receptor, metabotropic 4), 유전자등록번호(Genebank) X99975(H.sapiens mRNA for hRTR/hGCNF protein.), 유전자등록번호(Genebank) CR624693(3-hydroxyanthranilate 3,4-dioxygenase), 유전자등록번호(Genebank) AF227948(Hepatitis B virus x associated protein), 유전자등록번호(Genebank) AL834223(Histone deacetylase 11), 유전자등록번호(Genebank) NM_021170[Hairy and enhancer of split 4(Drosophila)], 유전자등록번호(Genebank) AK075040[Hairy and enhancer of split 6(Drosophila)], 유전자등록번호(Genebank) BC101593(Histone 1, H1a), 유전자등록 번호(Genebank) BU603483(Histone 1, H1e), 유전자등록번호(Genebank) BC065757(CDNA clone IMAGE:4556546), 유전자등록번호(Genebank) AK091904(CDNA FLJ34585 fis, clone KIDNE2008758), 유전자등록번호(Genebank) AK094407(CDNA FLJ37088 fis, clone BRACE2017124), 유전자등록번호(Genebank) AK097139(unnamed protein product; Homo sapiens cDNA FLJ39820 fis, clone SPLEN2010625.), 유전자등록번호(Genebank) AK124402(Similar to Olfactory receptor 4F3), 유전자등록번호(Genebank) AK024680(CDNA: FLJ21027 fis, clone CAE07110), 유전자등록번호(Genebank) AF040248(EDRF2; Homo sapiens erythroid differentiation-related factor 2 mRNA, partial cds.), 유전자등록번호(Genebank) S79672, 유전자등록번호(Genebank) AA995107[Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor(Pan troglodytes)], 유전자등록번호(Genebank)AI742278(Transcribed locus), 유전자등록번호(Genebank) AK124873[MRNA; cDNA DKFZp547G036(from clone DKFZp547G036)], 유전자등록번호(Genebank) CR936602[MRNA; cDNA DKFZp686L1223(from clone DKFZp686L1223)], 유전자등록번호(Genebank) BX091356(Surfactant associated protein F mRNA, partial sequence), 유전자등록번호(Genebank) AF147727(Homo sapiens unknown mRNA.), 유전자등록번호(Genebank) BC018035(CDNA clone IMAGE:4819956), 유전자등록번호(Genebank) CR622298[HMT1 hnRNP methyltransferase-like 2(S. cerevisiae)], 유전자등록번호(Genebank) NM_153456(Heparan sulfate 6-O-sulfotransferase 3), 유전자등록번호(Genebank) AL834347(Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7), 유전자등록번호(Genebank) NM_014157(HSPC065 protein), 유전자등록번호(Genebank) AL713654[pantothenate kinase 2(Hallervorden-Spatz syndrome)], 유전자등록번호(Genebank) NM_213621[5-hydroxytryptamine(serotonin) receptor 3A], 유전자등록번호(Genebank) BG284570(Transcribed locus), 유전자등록번호(Genebank) BC026338(Intercellular adhesion molecule 5, telencephalin), 유전자등록번호(Genebank) NM_015259(Inducible T-cell co-stimulator ligand), 유전자등록번호(Genebank) BM906235(Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), 유전자등록번호(Genebank) NM_014002(Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon), 유전자등록번호(Genebank) NM_000575(Interleukin 1, alpha), 유전자등록번호(Genebank) BC008678(Interleukin 1, beta), 유전자등록번호(Genebank) NM_000600[Interleukin 6(interferon, beta 2)], 유전자등록번호(Genebank) NM_178229(IQ motif containing GTPase activating protein 3), 유전자등록번호(Genebank) U52682(Interferon regulatory factor 4), 유전자등록번호(Genebank) AB209622(IL2-inducible T-cell kinase), 유전자등록번호(Genebank) AL049799(KIAA0040), 유전자등록번호(Genebank) BC002872(KIAA0515), 유전자등록번호(Genebank) AB028978(TBC1 domain family, member 2B), 유전자등록번호(Genebank) AB037748(KIAA1327 protein), 유전자등록번호(Genebank) NM_058237(KIAA1622), 유전자등록번XM_376018(KIAA1644 protein), 유전자등록번 호(Genebank) XM_372193(KIAA1751), 유전자등록번호(Genebank) NM_015254(Kinesin family member 13B), 유전자등록번호(Genebank) AB007918(Kinesin family member 21B), 유전자등록번호(Genebank) NM_153443(Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1), 유전자등록번호(Genebank) AY302131(Kin of IRRE like(Drosophila)), 유전자등록번호(Genebank) BC033580[Kruppel-like factor 1(erythroid)], 유전자등록번호(Genebank) NM_001002231(Kallikrein 2, prostatic), 유전자등록번호(Genebank) NM_002260(Killer cell lectin-like receptor subfamily C, member 2), 유전자등록번호(Genebank) NM_012316[Karyopherin alpha 6(importin alpha 7)], 유전자등록번호(Genebank) BC063625(Keratin associated protein 2-4), 유전자등록번호(Genebank) BQ691496(Keratin associated protein 4-10), 유전자등록번호(Genebank) NM_000425(L1 cell adhesion molecule), 유전자등록번호(Genebank) NM_198129(Laminin, alpha 3), 유전자등록번호(Genebank) NM_178354(Late cornified envelope 1F), 유전자등록번호(Genebank) NM_002298[Lymphocyte cytosolic protein 1(L-plastin)], 유전자등록번호(Genebank) NM_002309(Hypothetical protein MGC20647), 유전자등록번호(Genebank) XM_292512(Hypothetical protein LOC146517), 유전자등록번호(Genebank) AK091946(Hypothetical protein LOC283337), 유전자등록번호(Genebank) BC035704(Hypothetical protein LOC284352), 유전자등록번호(Genebank) NM_001017995(KIAA1295), 유전자등록번호(Genebank) AK094356(Hypothetical protein LOC286382), 유전자등록번호(Genebank) XM_291200(Similar to BC060615 protein), 유전자등록번호(Genebank) BC103740(Low density lipoprotein receptor class A domain containing 1), 유전자등록번호(Genebank) XM_375549(Hypothetical LOC400664), 유전자등록번호(Genebank) XM_379036(Hypothetical LOC400890), 유전자등록번호(Genebank) XM_498732(Hypothetical protein LOC115110), 유전자등록번호(Genebank) AK125737(LOC440570), 유전자등록번호(Genebank) XM_496400(Similar to hypothetical protein KIAA0454 - human(fragment)), 유전자등록번호(Genebank) BC094697(Hypothetical protein BC007901), 유전자등록번호(Genebank) NM_000237(Lipoprotein lipase), 유전자등록번호(Genebank) AK024955(Leucine rich repeat containing 19), 유전자등록번호(Genebank) AY358431(C-type lectin superfamily 4, member G), 유전자등록번호(Genebank) NM_002341[Lymphotoxin beta(TNF superfamily, member 3)], 유전자등록번호(Genebank) NM_000428(Latent transforming growth factor beta binding protein 2), 유전자등록번호(Genebank) AK024477(Latent transforming growth factor beta binding protein 3), 유전자등록번호(Genebank) NM_005461[V-maf musculoaponeurotic fibrosarcoma oncogene homolog B(avian)], 유전자등록번호(Genebank) AK092414[V-maf musculoaponeurotic fibrosarcoma oncogene homolog K(avian)], 유전자등록번호(Genebank) NM_138703(Melanoma antigen family E, 2), 유전자등록번호(Genebank) CR749534(Mannosidase, alpha, class 1B, member 1), 유전자등록번호(Genebank) NM_005764(PDZK1 interacting protein 1), 유전자등록번호(Genebank) NM_002375(Microtubule-associated protein 4), 유전자등록번호(Genebank) NM_030583(Matrilin 2), 유전자등록번호(Genebank) CR627018(Myelin basic protein), 유전자등록번호(Genebank) AK058070[Malate dehydrogenase 1B, NAD(soluble)], 유전자등록번호(Genebank)AK000052[Mediator of RNA polymerase II transcription, subunit 18 homolog(yeast)], 유전자등록번호(Genebank) AK128256[MADS box transcription enhancer factor 2, polypeptide B(myocyte enhancer factor 2B)], 유전자등록번호(Genebank) NM_005929[Antigen p97(melanoma associated) identified by monoclonal antibodies 133.2 and 96.5], 유전자등록번호(Genebank) AB235153[Mannosyl(alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B], 유전자등록번호(Genebank)AK075342(Hypothetical protein MGC10946), 유전자등록번호(Genebank) BC002462(Coiled-coil domain containing 28B), 유전자등록번호(Genebank) BC009980(Kelch domain containing 7B), 유전자등록번호(Genebank) BX647942(Likely ortholog of mouse schlafen 5), 유전자등록번호(Genebank) AY358435(Hypothetical protein MGC21688), 유전자등록번호(Genebank) BC004968(Hypothetical protein MGC2664), 유전자등록번호(Genebank) AK125168(Hypothetical protein MGC5356), 유전자등록번호(Genebank) NM_001620[AHNAK nucleoprotein(desmoyokin)], 유전자등록번호(Genebank) NM_014632(Microtubule associated monoxygenase, calponin and LIM domain containing 2), 유전자등록번호(Genebank) S77878, 유전자등록번호(Genebank) NM_003482(Myeloid/lymphoid or mixed-lineage leukemia 2), 유전자등록번호(Genebank) U38320(Matrix metallopeptidase 19), 유전자등록번호(Genebank) NM_004530[Matrix metallopeptidase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)], 유전자등록번호(Genebank) AJ298317(Mucin 5, subtypes A and C, tracheobronchial/gastric), 유전자등록번호(Genebank) NM_002471[Myosin, heavy polypeptide 6, cardiac muscle, alpha(cardiomyopathy, hypertrophic 1)], 유전자등록번호(Genebank)AB209997(Myosin IXB), 유전자등록번호(Genebank) NM_005967[NGFI-A binding protein 2(EGR1 binding protein 2)], 유전자등록번호(Genebank) AY358815(Neural cell adhesion molecule 1), 유전자등록번호(Genebank) AB082526[NIMA(never in mitosis gene a)- related kinase 9], 유전자등록번호(Genebank) AK055224(Neurogenic differentiation 6), 유전자등록번호(Genebank) NM_006162(Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1), 유전자등록번호(Genebank)AK090459(Nuclear factor(erythroid-derived 2)-like 1), 유전자등록번호(Genebank) AK074071(Sperm equatorial segment protein 1), 유전자등록번호(Genebank) AK160377(Nuclear pore complex interacting protein), 유전자등록번호(Genebank) NM_003873(Neuropilin 1), 유전자등록번호(Genebank) AL833606(Neuropilin 2), 유전자등록번호(Genebank) NM_001007156(Neurotrophic tyrosine kinase, receptor, type 3), 유전자등록번호(Genebank) NM_015231(Nucleoporin 160kDa), 유전자등록번호(Genebank) BC101462(Neurexophilin 2), 유전자등록번호(Genebank) XM_371717[Odz, odd Oz/ten-m homolog 3(Drosophila)], 유전자등록번호(Genebank) AI791380[Transcribed locus, weakly similar to NP_079012.2 gasdermin domain containing 1; 1810036L03Rik(Homo sapiens)], 유전자등록번호(Genebank) BC051352(Oligodendrocyte transcription factor 3), 유전자등록번호(Genebank) NM_001004751(Olfactory receptor, family 51, subfamily D, member 1), 유전자등록번호(Genebank) BG284742[P8 protein(candidate of metastasis 1)], 유전자등록번호(Genebank) NM_001618[Poly(ADP-ribose) polymerase family, member 1], 유전자등록번호(Genebank) BC035485[Poly(ADP-ribose) polymerase family, member 10], 유전자등록번호(Genebank) NM_032402(Protocadherin gamma subfamily A, 12), 유전자등록번호(Genebank) XM_374898[Pecanex-like 3(Drosophila)], 유전자등록번호(Genebank) AK001872(Programmed cell death 1 ligand 2), 유전자등록번호(Genebank)AB209081[Phosphodiesterase 4B, cAMP-specific(phosphodiesterase E4 dunce homolog, Drosophila)], 유전자등록번호(Genebank) AB094096(PDZ domain containing 2), 유전자등록번호(Genebank) AK127016(PDZ domain containing 4), 유전자등록번호(Genebank) BX648284[Pelota homolog(Drosophila)], 유전자등록번호(Genebank) AF289554(PERQ amino acid rich, with GYF domain 1), 유전자등록번호(Genebank) NM_006212(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2), 유전자등록번호(Genebank) NM_003629[Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)], 유전자등록번호(Genebank) NM_013440(Paired immunoglobin-like type 2 receptor beta), 유전자등록번호(Genebank) NM_052892(Polycystic kidney disease 1-like 2), 유전자등록번호(Genebank) NM_201382(Plectin 1, intermediate filament binding protein 500kDa), 유전자등록번호(Genebank) NM_032882(Paraneoplastic antigen like 6A), 유전자등록번호(Genebank) NM_00093[Polymerase(RNA) II(DNA directed) polypeptide A, 220kDa], 유전자등록번호(Genebank) NM_000944[(Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)], 유전자등록번호(Genebank) BQ073453(Protein kinase C, delta binding protein), 유전자등록번호(Genebank) NM_014502[PRP19/PSO4 pre-mRNA processing factor 19 homolog(S. cerevisiae)], 유전자등록번호(Genebank) M23575(Pregnancyspecific beta-1-glycoprotein 3), 유전자등록번호(Genebank) NM_002825[Pleiotrophin(heparin binding growth factor 8, neurite growth-promoting factor 1)], 유전자등록번호(Genebank) NM_002843(Protein tyrosine phosphatase, receptor type, J), 유전자등록번호(Genebank) NM_002852(Pentraxin-related gene, rapidly induced by IL-1 beta), 유전자등록번호(Genebank) BC017045(Phosphorylase, glycogen; brain), 유전자등록번호(Genebank) BM974642(Transcribed locus), 유전자등록번호(Genebank) NM_002826(Quiescin Q6), 유전자등록번호(Genebank) NM_181701(Quiescin Q6-like 1), 유전자등록번호(Genebank) AF329499(RAB27B, member RAS oncogene family), 유전자등록번호(Genebank) CR620019(RAB43, member RAS oncogene family), 유전자등록번호(Genebank) NM_198679[Rap guanine nucleotide exchange factor(GEF) 1], 유전자등록번호(Genebank) NM_002888[Retinoic acid receptor responder(tazarotene induced) 1], 유전자등록번호(Genebank) AK092804[Retinoic acid receptor responder(tazarotene induced) 2], 유전자등록번호(Genebank) X89399(RAS p21 protein activator 3), 유전자등록번호(Genebank) NM_002891(Ras protein-specific guanine nucleotide-releasing factor 1), 유전자등록번호(Genebank) NM_002891(Ras protein-specific guanine nucleotide-releasing factor 1), 유전자등록번호(Genebank) AK128127(Regulator of G-protein signalling 3), 유전자등록번호(Genebank) BM980591(Transcribed locus), 유전자등록번호(Genebank) BG564669(Serum amyloid A2), 유전자등록번호(Genebank) NM_030754(Serum amyloid A2), 유전자등록번호(Genebank) BG564326(Serum amyloid A4, constitutive), 유전자등록번호(Genebank) BC022509[Secretogranin II(chromogranin C)], 유전자등록번호(Genebank) NM_006514(Sodium channel, voltage-gated, type X, alpha), 유전자등록번호(Genebank) NM_198056[Sodium channel, voltage-gated, type V, alpha(long QT syndrome 3)], 유전자등록번호(Genebank) AB007937[Syndecan 3(N-syndecan)], 유전자등록번호(Genebank) NM_002999[Syndecan 4(amphiglycan, ryudocan)], 유전자등록번호(Genebank) AK122782(Orphan short-chain dehydrogenase / reductase), 유전자등록번호(Genebank) NM_003004(Secreted and transmembrane 1), 유전자등록번호(Genebank)NM_152754[Sema domain, immunoglobulin domain(Ig), short basic domain, secreted,(semaphorin) 3D], 유전자등록번호(Genebank) AK093049[Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3], 유전자등록번호(Genebank) AK058094(Surfactant, pulmonary-associated protein C), 유전자등록번호(Genebank)NM_144579(Sideroflexin 5), 유전자등록번호(Genebank)AK074131(SH3 multiple domains 4), 유전자등록번호(Genebank) BC098390[Solute carrier family 12(potassium/chloride transporters), member 7], 유전자등록번호(Genebank) AK091926(Solute carrier family 13(sodium-dependent dicarboxylate transporter), member 2), 유전자등록번호(Genebank) AK127216(Solute carrier family 15, member 3), 유전자등록번호(Genebank) NM_004172[Solutecarrier family 1(glial high affinity glutamate transporter], member 3), 유전자등록번호(Genebank) NM_153320[Solute carrier family 22(organic anion transporter), member 7], 유전자등록번호(Genebank) NM_203327[Solute carrier family 23(nucleobase transporters), member 2], 유전자등록번호(Genebank) NM_152346(Solute carrier family 43, member 2), 유전자등록번호(Genebank) NM_201649[Solute carrier family 6(neurotransmitter transporter, glycine), member 9], 유전자등록번호(Genebank) NM_003045[Solute carrier family 7(cationic amino acid transporter, y+ system), member 1], 유전자등록번호(Genebank) NM_003486[Solute carrier family 7(cationic amino acid transporter, y+ system), member 5], 유전자등록번호(Genebank) BC098388[Slit homolog 3(Drosophila)], 유전자등록번호(Genebank)NM_139067(SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2), 유전자등록번호(Genebank) AK127286(Spermine oxidase), 유전자등록번 호(Genebank) NM_003955(Suppressor of cytokine signaling 3), 유전자등록번호(Genebank) NM_003105(Chromosome 11 open reading frame 32), 유전자등록번호(Genebank) NM_003106[SRY(sex determining region Y)-box 2], 유전자등록번호(Genebank) NM_173360(Spermatogenesis associated 9), 유전자등록번호(Genebank) BC017222(Sequestosome 1), 유전자등록번호(Genebank) NM_080836(Serine/threonine kinase 35), 유전자등록번호(Genebank) AF545571(Sulfatase 1), 유전자등록번호(Genebank) NM_177963(Synaptotagmin XII), 유전자등록번호(Genebank) NM_000545[Transcription factor 1, hepatic; LF-B1, hepatic nuclear factor(HNF1), albumin proximal factor], 유전자등록번호(Genebank) NM_000358(Transforming growth factor, beta-induced, 68kDa), 유전자등록번호(Genebank) NM_015335(Thyroid hormone receptor associated protein 2), 유전자등록번호(Genebank) AB046767[Transducin-like enhancer of split 3(E(sp1) homolog, Drosophila)], 유전자등록번호(Genebank)AF367761(Transmembrane protein 10), 유전자등록번호(Genebank) NM_002160(Tenascin C(hexabrachion)), 유전자등록번호(Genebank) BC028148[Tumor necrosis factor(TNF superfamily, member 2)], 유전자등록번호(Genebank) NM_006291(Tumor necrosis factor, alpha-induced protein 2), 유전자등록번호(Genebank) NM_006290(Tumor necrosis factor, alpha-induced protein 3), 유전자등록번호(Genebank) NM_007115(Tumor necrosis factor, alpha-induced protein 6), 유전자등록번호(Genebank) BC052977(Tumor necrosis factor receptor superfamily, member 1B), 유전자등록번호(Genebank) NM_003811[Tumor necrosis factor(ligand) superfamily, member 9], 유전자등록번호(Genebank) AB210011[Transportin 2(importin 3, karyopherin beta 2b)], 유전자등록번호(Genebank) NM_005427(Tumor protein p73), 유전자등록번호(Genebank) AB032995(Two pore segment channel 1), 유전자등록번호(Genebank) S76980[hexosaminidase A(alpha polypeptide)], 유전자등록번호(Genebank) NM_003320[Tubby homolog(mouse)], 유전자등록번호(Genebank) NM_003328(TXK tyrosine kinase), 유전자등록번호(Genebank) NM_006472(Thioredoxin interacting protein), 유전자등록번호(Genebank) NM_003360[UDP glycosyltransferase 8(UDP-galactose ceramide galactosyltransferase)], 유전자등록번호(Genebank) NM_025154[Unc-84 homolog A(C. elegans)], 유전자등록번호(Genebank) BC094797[Unc-84 homolog B(C. elegans)], 유전자등록번호(Genebank) AW016806(Transcribed locus), 유전자등록번호(Genebank) AW341701(Transcribed locus), 유전자등록번호(Genebank) NM_199511(Steroid sensitive gene 1), 유전자등록번호(Genebank) DQ097177(HECT, UBA and WWE domain containing 1), 유전자등록번호(Genebank) BC027954(Tcell receptor gamma variable 7), 유전자등록번호(Genebank) NM_003371(Vav 2 oncogene), 유전자등록번호(Genebank) AF167081(Variable charge, X-linked), 유전자등록번호(Genebank) AK058041[Vestigial like 4(Drosophila)], 유전자등록번호(Genebank) AJ132099(Vanin 1), 유전자등록번호(Genebank) NM_014588[Visual system homeobox 1 homolog, CHX10-like(zebrafish)], 유전자등록번호(Genebank) NM_000638[Vitronectin(serum spreading factor, somatomedin B, complement S-protein)], 유전자등록번호(Genebank) NM_022170(Myosin XVB pseudogene), 유전자등록번호(Genebank) NM_032951(MLX interacting protein-like), 유전자등록번호(Genebank) BC052234(Wingless-type MMTV integration site family, member 10A), 유전자등록번호(Genebank) AK126706(X-ray repair complementing defective repair in Chinese hamster cells 3), 유전자등록번호(Genebank) NM_020205(Zinc finger, A20 domain containing 1), 유전자등록번호(Genebank) D26122(splicing factor 1), 유전자등록번호(Genebank) NM_032441(Zinc finger, matrin type 1), 유전자등록번호(Genebank) BC036823(Zinc finger protein 289, ID1 regulated), 유전자등록번호(Genebank) NM_182975(Zinc finger protein 326), 유전자등록번호(Genebank) NM_181489(Zinc finger protein 445), 유전자등록번호(Genebank) BG201273[Zinc finger protein 75(D8C6)], 유전자등록번호(Genebank) AY008294[Zinc finger protein, subfamily 1A, 2(Helios)].Genebank (Genebank) NM_007313 (V-abl Abelson murine leukemia viral oncogene homolog 1), Genebank (Genebank) NM_203380 (Acyl-CoA synthetase long-chain family member 5), Genebank (BCenebank) BC040531 (Activin A receptor, type IB), Genebank Y13786 [ADAM metallopeptidase domain 19 (meltrin beta)], Genebank number AJ250725 (ADAM metallopeptidase with thrombospondin type 1 motif, 12), Gene registration number ( Genebank) AJ315733 (ADAM metallopeptidase with thrombospondin type 1 motif, 15), Gene Registration Number (Genebank) NM_133638 (ADAM metallopeptidase with thrombospondin type 1 motif, 19), Gene Registration Number (Genebank) NM_014243 (ADAM metallopeptidase with thrombospondin type 1 motif, 3), Genebank NM_207517 (ADAMTS-like 3), Genebank (Genebank) BC013780 (Adenosine A2a receptor), Genebank AK160382 (AT-hook transcription factor), Genebank (Genebank) ) AK12 2839 (V-akt murine thymoma viral oncogene homolog 2), Genebank AK128709 (Aldehyde dehydrogenase 1 family, member A2), Genebank number NM_003748 (Aldehyde dehydrogenase 4 family, member A1), Gene Registration Number (Genebank) NM_172196 (TFIIA-alpha / beta-like factor), Genebank NM_152526 [Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 19], Genebank NM_139314 (Angiopoietin-like 4) , Genebank No. NM_000037 (Ankyrin 1, erythrocytic), Genebank No. AJ583444 [Ankyrin repeat domain 2 (stretch responsive muscle)], Genebank No. NM_145641 (Apolipoprotein L, 3), Gene Registration Genebank BC006276 (Apolipoprotein L, 4), Genebank NM_003224 (ADP-ribosylation factor related protein 1), Genebank NM_021069 (Sorbin and SH3 domain containing 2), Genebank (Genebank) ) AK090447 (Rho GTPase activating pr otein 19), Genebank NM_015071 (Rho GTPase activating protein 26), Genebank NM_032199 [AT rich interactive domain 5B (MRF1-like)], Genebank NM_183376 (Arrestin domain containing 4), Genebank AK000643 (Ankyrin repeat and SOCS box-containing 9), Genebank (Genebank) AB209032 (Activating transcription factor 3), Genebank (Genebank) NM_032827 [Atonal homolog 8 (Drosophila) ], Genebank BX537444 (ATPase, Ca ++ transporting, plasma membrane 4), Genebank (Genebank) NM_006045 (ATPase, Class II, type 9A), Genebank AB011106 (Attractin-like 1) , Genebank No. NM_000707 (Arginine vasopressin receptor 1B), Genebank No. CR933657 [Axin 2 (conductin, axil)], Genebank No. NM_152384 (Bardet-Biedl syndrome 5), Gene Registration No. (Genebank) NM_021948 (Brevican), Genebank AA60 8624 (Breast carcinoma amplified sequence 3), gene registration number (Genebank) NM_017745 (BCL6 co-repressor), gene registration number (Genebank) S72478, gene registration number (Genebank) NM_001710 (B-factor, properdin), gene registration number ( Genebank) CR749373 (chromosome 1 open reading frame 178), Genebank AL713697 (Bridging integrator 1), Genebank NM_001165 (Baculoviral IAP repeat-containing 3), Genebank BM984396 (Plasminogen -like B2), Genebank NM_001203 (Bone morphogenetic protein receptor, type IB), Genebank AK098095 (V-raf murine sarcoma viral oncogene homolog B1), Genebank NM_014299 ( Bromodomain containing 4), Genebank BC016743 [BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S.  cerevisiae)], Genebank NM_001728 [Basigin (OK blood group)], Genebank NM_052876 [BTB (POZ) domain containing 14B], Genebank BX537534 [BTB (POZ) domain containing 9], Genebank BX107768 (Transcribed locus), Genebank AY665468 [Ras association (RalGDS / AF-6) domain family 8], Genebank NM_033513 (Chromosome 19 open reading frame 20), Genebank BC068439 (Chromosome 19 open reading frame 28), Genebank AY358424 (C1q and tumor necrosis factor related protein 1), Genebank NM_001733 (Complement component 1, r subcomponent, Genebank NM_080834 (Chromosome 20 open reading frame 152), Genebank AK000652 (Chromosome 20 open reading frame 57), Genebank BC063852 (Complement component 3), Gene Registration Number (Genebank) AF251038 (Chromosome 5 open read ing frame 5), Genebank B X640641 (Chromosome 6 open reading frame 107), Genebank number AL832416 (Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1), Gene Registration Number ( Genebank) BC021552 (Calcium channel, voltage-dependent, gamma subunit 6), Genebank number AL117636 (Calneuron 1), Genebank number (Genebank) AJ519841 (Calmodulin regulated spectrin-associated protein 1), Genebank number (Genebank) ) AK128124 (Calpain 5), Genebank NM_002982 [Chemokine (CC motif) ligand 2], Genebank (Genebank) BC020698 [Chemokine (CC motif) ligand 20], Genebank BC071718 [Chemokine (CC motif) ligand 4-like 1], gene registration number (Genebank) CR614015 (CD14 antigen), gene registration number (Genebank) NM_001004196 (CD200 antigen), gene registration number (Genebank) AK090470 [CD33 antigen (gp67)], Genebank (Genebank) NM_001773 (CD34 antigen), Gene Registration Number (Geneb) ank) BC025729 (CD99 antigen-like 2), Gene Registration Number (Genebank) AB051503 (Centaurin, beta 5), Gene Registration Number (Genebank) NM_032221 (Chromodomain helicase DNA binding protein 6), Gene Registration Number (Genebank) NM_000740 (Cholinergic receptor, muscarinic 3), gene registration number (Genebank) NM_016369 (Claudin 18), gene registration number (Genebank) AB007969 [Calmin (calponin-like, transmembrane)], gene registration number (Genebank) AJ278018 (Calsyntenin 2), gene registration Genebank AJ626900 (Cardiomyopathy associated 1), Genebank NM_020872 [Contactin 3 (plasmacytoma associated)], Genebank NM_004370 (Collagen, type XII, alpha 1), Genebank (Genebank) Z74615 (Collagen, type I, alpha 1), Genebank NM_001845 (Collagen, type IV, alpha 1), Genebank NM_033380 [Collagen, type IV, alpha 5 (Alport syndrome)], gene Registration Number (Genebank) NM_000393 (Collagen, type V, alpha 2), Gene Registration Number (Gen ebank) NM_001848 (Collagen, type VI, alpha 1), Genebank NM_001850 (Collagen, type VIII, alpha 1), Genebank AK124894 (Corticotropin releasing hormone receptor 1), Genebank (Genebank) ) BC021117 [Colony stimulating factor 1 (macrophage)], gene registration number (Genebank) BE873976 [Colony stimulating factor 2 (granulocyte-macrophage)], gene registration number (Genebank) NM_172220 [Colony stimulating factor 3 (granulocyte)], gene registration Number (Genebank) AK092391 (Cystatin E / M), Gene Registration Number (Genebank) NM_147780 (Cathepsin B), Gene Registration Number (Genebank) NM_001334 (Cathepsin O), Gene Registration Number (Genebank) NM_198700 (CUG triplet repeat, RNA binding protein 1), Genebank NM_015267 [Cut-like 2 (Drosophila)], Genebank NM_001511 [Chemokine (CXC motif) ligand 1 (melanoma growth stimulating activity, alpha)], Gene registry number ( Genebank) BC053653 [Chemokine (CXC motif) ligand 2], gene registration Genebank NM_002090 [Chemokine (CXC motif) ligand 3], Genebank NM_002994 [Chemokine (CXC motif) ligand 5], Genebank U81234 [Chemokine (CXC motif) ligand 6 (granulocyte chemotactic protein 2)], Genebank NM_000497 (Cytochrome P450, family 11, subfamily B, polypeptide 1), Genebank NM_004393 [Dystroglycan 1 (dystrophin-associated glycoprotein 1)], Genebank (Genebank) ) BX537651 (Discoidin domain receptor family, member 2), Genebank AB209635 (Diacylglycerol kinase, zeta 104kDa), Genebank AB209653 [Dehydrogenase / reductase (SDR family) member 2], Gene registration number ( Genebank) NM_004753 [Dehydrogenase / reductase (SDR family) member 3], Gene Registration Number (Genebank) AL832637 (Taxilin alpha), Gene Registration Number (Genebank) BX640676 (Regeneration associated muscle protease), Gene Registration Number (Genebank) XM_291222 (Integrator complex subunit 1), heredity Genebank NM_031294 (Leucine rich repeat containing 48), Gene Registration Number (Genebank) NM_182643 (Deleted in liver cancer 1), Gene Registration Number (Genebank) NM_001365 [Discs, large homolog 4 (Drosophila)], Gene registration Genebank NM_007329 (Deleted in malignant brain tumors 1), Genebank BM808864 (Dopamine receptor D1 interacting protein), Genebank AK057845 (Ephrin-A1), Genebank NM_012199 ( Eukaryotic translation initiation factor 2C, 1), Genebank BX537368 [E74-like factor 3 (ets domain transcription factor, epithelial-specific)], Genebank NM_001426 (Engrailed homolog 1), Gene accession number (Genebank) BC054508 (Erythrocyte membrane protein band 4. 1 like 5), Gene Registration Number (Genebank) AF073727 (Epsin 1), Gene Registration Number (Genebank) BC060883 (Epsin 2), Gene Registration Number (Genebank) AL831953 [Epithelial stromal interaction 1 (breast)], Gene Registration Number ( Genebank) D79987 [Extra spindle poles like 1 (S.  cerevisiae)], Genebank NM_00198 [Ets variant gene 6 (TEL oncogene)], Genebank BX648769 (Ewing sarcoma breakpoint region 1), Genebank NM_014661 (Family with sequence similarity 53, member B), Genebank (Genebank) NM_001004019 (Fibulin 2), Genebank (Genebank) AK125888 (F-box protein 32), Genebank (Genebank) NM_002016 (Filaggrin), Genebank (Genebank) NM_024669 (FLJ11795 protein), Genebank Number (Genebank) NM_024817 (Hypothetical protein FLJ13710), Gene Registration Number (Genebank) NM_017631 (Hypothetical protein FLJ20035), Genebank Number BQ279256 (Hypothetical protein FLJ20294), Gene Registration Number (Genebank) ) AK123641 (Cleavage and polyadenylation specific factor 3-like), Genebank AK024488 (Hypothetical protein FLJ21438), Genebank AL832659 (Hypothetical protein FLJ22833), Genebank NM_181645 (Hypothe tical protein FLJ25393), Genebank AL833626 (Zinc finger, DHHC-type containing 20), Genebank (Genebank) NM_173621 (Chromosome 17 open reading frame 44), Genebank NM_001460 (Flavin containing monooxygenase 2), Genebank NM_001461 (Flavin containing monooxygenase 5), Genebank NG_212482 (Fibronectin 1), Genebank AB033068 [Fizzy / cell division cycle 20 related 1 (Drosophila)], Genebank NM_080612 (GRB2-associated binding protein 3), Genebank BC051463 [Gamma-aminobutyric acid (GABA) B receptor, 1], Genebank NM_198321 [UDP-N- acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)], Genebank NM_198335 (Glucosidase, alpha; neutral AB), Genebank NM_002048 (Growth arrest-specific 1), Genebank (Genebank) AK127845 (GATA binding protein 2), Genebank NM_000514 (Glial cell derived neurotrophic factor), Gene registration Number (Genebank) NM_000165 [Gap junction protein, alpha 1, 43 kDa (connexin 43)], gene registration number (Genebank) BC043390 (Glucagon-like peptide 2 receptor), gene registration number (Genebank) AF288389 (Glycosyltransferase 25 domain containing 2) , Genebank BC051726 [Glutamate-ammonia ligase (glutamine synthetase)], Genebank (Genebank) CR936770 [Guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O], Genebank (Genebank) BC095544 (G protein-coupled receptor 32), Genebank NM_000841 (Glutamate receptor, metabotropic 4), Genebank X99975 (H. sapiens mRNA for hRTR / hGCNF protein. ), Genebank CR624693 (3-hydroxyanthranilate 3,4-dioxygenase), Genebank AF227948 (Hepatitis B virus x associated protein), Genebank AL834223 (Histone deacetylase 11), Gene Genebank NM_021170 [Hairy and enhancer of split 4 (Drosophila)], Gene accession number (Genebank) AK075040 [Hairy and enhancer of split 6 (Drosophila)], Gene accession number (Genebank) BC101593 (Histone 1, H1a) , Gene Registration Number (Genebank) BU603483 (Histone 1, H1e), Gene Registration Number (Genebank) BC065757 (CDNA clone IMAGE: 4556546), Gene Registration Number (Genebank) AK091904 (CDNA FLJ34585 fis, clone KIDNE2008758), Gene Registration Number ( Genebank) AK094407 (CDNA FLJ37088 fis, clone BRACE2017124), Genebank AK097139 (unnamed protein product; Homo sapiens cDNA FLJ39820 fis, clone SPLEN2010625. Genebank AK124402 (Similar to Olfactory receptor 4F3), Genebank AK024680 (CDNA: FLJ21027 fis, clone CAE07110), Genebank AF040248 (EDRF2; Homo sapiens erythroid differentiation-related factor 2 mRNA, partial cds. ), Genebank S79672, Genebank AA995107 [Transcribed locus, moderately similar to XP_517807. 1 PREDICTED: interleukin 7 receptor (Pan troglodytes), Genebank AI742278 (Transcribed locus), Genebank AK124873 [MRNA; cDNA DKFZp547G036 (from clone DKFZp547G036)], Genebank CR936602 [MRNA; cDNA DKFZp686L1223 (from clone DKFZp686L1223), Genebank BX091356 (Surfactant associated protein F mRNA, partial sequence), Genebank AF147727 (Homo sapiens unknown mRNA. ), Genebank BC018035 (CDNA clone IMAGE: 4819956), Genebank CR622298 [HMT1 hnRNP methyltransferase-like 2 (S.  cerevisiae)], Genebank NM_153456 (Heparan sulfate 6-O-sulfotransferase 3), Genebank AL834347 (Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7), Genebank NM_014157 (HSPC065 protein), Genebank AL713654 [pantothenate kinase 2 (Hallervorden-Spatz syndrome)], Genebank NM_213621 [5-hydroxytryptamine (serotonin) receptor 3A], gene Genebank BG284570 (Transcribed locus), Genebank (Genebank) BC026338 (Intercellular adhesion molecule 5, telencephalin), Genebank (Genebank) NM_015259 (Inducible T-cell co-stimulator ligand), Genebank (Genebank) ) BM906235 (Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), Genebank NM_014002 (Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon), Genebank NM_000575 (Interleukin 1, alpha), you Genebank BC008678 (Interleukin 1, beta), Genebank NM_000600 [Interleukin 6 (interferon, beta 2)], Genebank NM_178229 (IQ motif containing GTPase activating protein 3), Gene Registration Number (Genebank) U52682 (Interferon regulatory factor 4), Gene Registration Number (Genebank) AB209622 (IL2-inducible T-cell kinase), Gene Registration Number (Genebank) AL049799 (KIAA0040), Gene Registration Number (Genebank) BC002872 (KIAA0515 ), Gene registration number (Genebank) AB028978 (TBC1 domain family, member 2B), gene registration number (Genebank) AB037748 (KIAA1327 protein), gene registration number (Genebank) NM_058237 (KIAA1622), gene registration number XM_376018 (KIAA1644 protein), Gene Registration Number (Genebank) XM_372193 (KIAA1751), Gene Registration Number (Genebank) NM_015254 (Kinesin family member 13B), Gene Registration Number (Genebank) AB007918 (Kinesin family member 21B), Gene Registration Number (Genebank) NM_153443 (Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1), Genebank AY302131 (Kin of IRRE like (Drosophila)), Genebank (Genebank) BC033580 [Kruppel-like factor 1 (erythroid)], Genebank NM_001002231 ( Kallikrein 2, prostatic), Genebank NM_002260 (Killer cell lectin-like receptor subfamily C, member 2), Genebank NM_012316 [Karyopherin alpha 6 (importin alpha 7)], Genebank (Genebank) ) BC063625 (Keratin associated protein 2-4), Genebank (Genebank) BQ691496 (Keratin associated protein 4-10), Genebank (Genebank) NM_000425 (L1 cell adhesion molecule), Genebank (Genebank) NM_198129 (Laminin , alpha 3), Genebank NM_178354 (Late cornified envelope 1F), Genebank NM_002298 [Lymphocyte cytosolic protein 1 (L-plastin)], Genebank NM_002309 (Hypothetical protein MGC20647) , Genebank XM_292512 (Hypothetical protein LOC146517), Genebank Number AK091946 (Hypothetical protein LOC283337), Genebank Number (Genebank) BC035704 (Hypothetical protein LOC284352), Genebank Number NM_001017995 (KIAA1295), Genebank Number AK094356 protein (Hybank) LOC286382), Genebank XM_291200 (Similar to BC060615 protein), Genebank (Genebank) BC103740 (Low density lipoprotein receptor class A domain containing 1), Genebank XM_375549 (Hypothetical LOC400664), Gene registration Number (Genebank) XM_379036 (Hypothetical LOC400890), Gene Registration Number (Genebank) XM_498732 (Hypothetical protein LOC115110), Gene Registration Number (Genebank) AK125737 (LOC440570), Gene Registration Number (Genebank) XM_496400 (Similar to hypothetical protein KIAA0454-human fragments), Genebank BC094697 (Hypothetical protein BC007901), Genebank NM_000237 (Lipoprotein lipase), Genebank (Genebank) AK024955 (Leucine rich repeat containing 19), Genebank AY358431 (C-type lectin superfamily 4, member G), Genebank NM_002341 [Lymphotoxin beta (TNF superfamily, member 3)], Gene registration number (Genebank) NM_000428 (Latent transforming growth factor beta binding protein 2), Gene accession number (Genebank) AK024477 (Latent transforming growth factor beta binding protein 3), Gene accession number (Genebank) NM_005461 [V-maf musculoaponeurotic fibrosarcoma oncogene homolog B ( avian)], Genebank AK092414 [V-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)], Genebank NM_138703 (Melanoma antigen family E, 2), Genebank CR749534 (Mannosidase) , alpha, class 1B, member 1), Genebank NM_005764 (PDZK1 interacting protein 1), Genebank NM_002375 (Microtubule-associated protein 4), Genebank NM_030583 (Matrilin 2) , gene Genebank CR627018 (Myelin basic protein), Genebank AK058070 [Malate dehydrogenase 1B, NAD (soluble)], Genebank (Kenebank) AK000052 [Mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) )], Genebank AK128256 [MADS box transcription enhancer factor 2, polypeptide B (myocyte enhancer factor 2B)], Genebank NM_005929 [Antigen p97 (melanoma associated) identified by monoclonal antibodies 133. 2 and 96. 5], Genebank AB235153 [Mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B], Genebank AK075342 (Hypothetical protein MGC10946) , Genebank Number BC002462 (Coiled-coil domain containing 28B), Genebank Number (Genebank) BC009980 (Kelch domain containing 7B), Genebank Number (Genebank) BX647942 (Likely ortholog of mouse schlafen 5), Gene Registration Number (Genebank) AY358435 (Hypothetical protein MGC21688), Genebank BC004968 (Hypothetical protein MGC2664), Genebank AK125168 (Hypothetical protein MGC5356), Genebank NM_001620 [AHNAyokin nucleoprotein] desmo , Gene registration number (Genebank) NM_014632 (Microtubule associated monoxygenase, calponin and LIM domain containing 2), Gene registration number (Genebank) S77878, Gene registration number (Genebank) NM_003482 (Myeloid / lymphoid or mixed-lineage leukemia 2), Gene registration Number (Geneb ank) U38320 (Matrix metallopeptidase 19), Genebank NM_004530 [Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)], Genebank AJ298317 (Mucin 5, subtypes A and C, tracheobronchial / gastric), Genebank NM_002471 [Myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1)], Genebank (Genebank) AB209997 (Myosin IXB), Genebank (Genebank) NM_005967 NGFI-A binding protein 2 (EGR1 binding protein 2), Genebank AY358815 (Neural cell adhesion molecule 1), Genebank AB082526 [never in mitosis gene a) -related kinase 9 ], Genebank AK055224 (Neurogenic differentiation 6), Genebank (Genebank) NM_006162 (Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1), Genebank (AKenebank) AK090459 (Nuclear factor ( erythroid-derived 2) -like 1), gene registration number Genebank AK074071 (Sperm equatorial segment protein 1), Genebank AK160377 (Nuclear pore complex interacting protein), Genebank NM_003873 (Neuropilin 1), Genebank AL833606 (Neuropilin 2) , Gene Registration Number (Genebank) NM_001007156 (Neurotrophic tyrosine kinase, receptor, type 3), Gene Registration Number (Genebank) NM_015231 (Nucleoporin 160kDa), Gene Registration Number (Genebank) BC101462 (Neurexophilin 2), Gene Registration Number (Genebank) XM_371717 Odz, odd Oz / ten-m homolog 3 (Drosophila), Genebank AI791380 [Transcribed locus, weakly similar to NP_079012. 2 gasdermin domain containing 1; 1810036L03Rik (Homo sapiens)], Genebank BC051352 (Oligodendrocyte transcription factor 3), Genebank (Genebank) NM_001004751 (Olfactory receptor, family 51, subfamily D, member 1), Genebank BG284742 [ P8 protein (candidate of metastasis 1)], Genebank NM_001618 [Poly (ADP-ribose) polymerase family, member 1], Genebank BC035485 [Poly (ADP-ribose) polymerase family, member 10 ], Genebank NM_032402 (Protocadherin gamma subfamily A, 12), Genebank XM_374898 [Pecanex-like 3 (Drosophila)], Genebank AK001872 (Programmed cell death 1 ligand 2) , Genebank AB209081 [Phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)], Genebank AB094096 (PDZ domain containing 2), Genebank AK127016 (PDZ domain containing 4), Genebank BX648284 [P elota homolog (Drosophila)], Genebank AF289554 (PERQ amino acid rich, with GYF domain 1), Genebank NM_006212 (6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 2 ), Genebank NM_003629 [Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma)], Genebank NM_013440 (Paired immunoglobin-like type 2 receptor beta), Genebank (Genebank) NM_052892 (Polycystic kidney disease 1-like 2), Genebank (Genebank) NM_201382 (Plectin 1, intermediate filament binding protein 500kDa), Genebank (Genebank) NM_032882 (Paraneoplastic antigen like 6A), Genebank (Genebank) NM_00093 [Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa], Genebank NM_000944 [(Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha)], Genebank (Genebank) ) BQ073453 (Protein kinase C, delta binding protein), gene registration number (Gene bank) NM_014502 [PRP19 / PSO4 pre-mRNA processing factor 19 homolog (S.  cerevisiae)], Genebank No. M23575 (Pregnancyspecific beta-1-glycoprotein 3), Genebank No. NM_002825 [Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)], Gene registration number ( Genebank) NM_002843 (Protein tyrosine phosphatase, receptor type, J), Genebank NM_002852 (Pentraxin-related gene, rapidly induced by IL-1 beta), Genebank BC017045 (Phosphorylase, glycogen; brain) , Gene Registration Number (Genebank) BM974642 (Transcribed locus), Gene Registration Number (Genebank) NM_002826 (Quiescin Q6), Gene Registration Number (Genebank) NM_181701 (Quiescin Q6-like 1), Gene Registration Number (Genebank) AF329499 (RAB27B, member RAS oncogene family, Genebank CR620019 (RAB43, member RAS oncogene family), Genebank NM_198679 [Rap guanine nucleotide exchange factor (GEF) 1], Genebank NM_002888 [Retinoic acid receptor responder (tazarotene induce) d) 1], Genebank AK092804 [Retinoic acid receptor responder (tazarotene induced) 2], Gene accession number (Genebank) X89399 (RAS p21 protein activator 3), Genebank number NM_002891 (Ras protein- Specific guanine nucleotide-releasing factor 1), Genebank NM_002891 (Ras protein-specific guanine nucleotide-releasing factor 1), Genebank AK128127 (Regulator of G-protein signaling 3), Gene registration number ( Genebank) BM980591 (Transcribed locus), Gene Registration Number (Genebank) BG564669 (Serum amyloid A2), Gene Registration Number (Genebank) NM_030754 (Serum amyloid A2), Gene Registration Number (Genebank) BG564326 (Serum amyloid A4, constitutive), Gene Registration Number (Genebank) BC022509 [Secretogranin II (chromogranin C)], Gene Registration Number (Genebank) NM_006514 (Sodium channel, voltage-gated, type X, alpha), Gene Registration Number (Genebank) NM_198056 [Sodium channel, voltage-gated , type V, alpha (long QT syndrome 3)], gene Genebank AB007937 [Syndecan 3 (N-syndecan)], Genebank (Genebank) NM_002999 [Syndecan 4 (amphiglycan, ryudocan)], Genebank (Kenebank) AK122782 (Orphan short-chain dehydrogenase / reductase), Genebank NM_003004 (Secreted and transmembrane 1), Genebank NM_152754 [Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D], Genebank (Kenebank) AK093049 [Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3], Genebank AK058094 (Surfactant, pulmonary-associated protein C), Genebank NM_144579 (Sideroflexin 5), gene Registration Number (Genebank) AK074131 (SH3 multiple domains 4), Gene Registration Number (Genebank) BC098390 [Solute carrier family 12 (potassium / chloride transporters), member 7], Gene Registration Number (Genebank) AK091926 (Solute carrier family 13 (sodium) -dependent dicarboxylate transporter), member 2), gene Genebank AK127216 (Solute carrier family 15, member 3), Genebank (Genebank) NM_004172 [Solutecarrier family 1 (glial high affinity glutamate transporter), member 3), Genebank (Menebank) NM_153320 [Solute carrier family 22 (organic anion transporter), member 7], Genebank NM_203327 [Solute carrier family 23 (nucleobase transporters), member 2], Genebank NM_152346 (Solute carrier family 43, member 2), Gene Genebank NM_201649 [Solute carrier family 6 (neurotransmitter transporter, glycine), member 9], Gene registration number (Genebank) NM_003045 [Solute carrier family 7 (cationic amino acid transporter, y + system), member 1], Gene registration Number (Genebank) NM_003486 [Solute carrier family 7 (cationic amino acid transporter, y + system), member 5], Gene accession number (Genebank) BC098388 [Slit homolog 3 (Drosophila)], Gene accession number (Genebank) NM_139067 (SWI / SNF related, matrix associated, actin dependen t regulator of chromatin, subfamily c, member 2), Genebank AK127286 (Spermine oxidase), Genebank NM_003955 (Suppressor of cytokine signaling 3), Genebank NM_003105 (Chromosome 11 open reading frame 32), Genebank NM_003106 [sex determining region Y] -box 2], Genebank NM_173360 (Spermatogenesis associated 9), Genebank BC017222 (Sequestosome 1) , Gene Registration Number (Genebank) NM_080836 (Serine / threonine kinase 35), Gene Registration Number (Genebank) AF545571 (Sulfatase 1), Gene Registration Number (Genebank) NM_177963 (Synaptotagmin XII), Gene Registration Number (Genebank) NM_000545 [Transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor], Genebank NM_000358 (Transforming growth factor, beta-induced, 68kDa), Genebank NM_015335 (Thyroid hormone receptor associated protein 2) , Genebank AB046767 [Transducin-like enhancer of split 3 (E (sp1) homolog, Drosophila)], Genebank (Genebank) AF367761 (Transmembrane protein 10), Genebank (Menebank) NM_002160 (Tenascin C) (hexabrachion), Genebank BC028148 [Tumor necrosis factor (TNF superfamily, member 2)], Genebank (Genebank) NM_006291 (Tumor necrosis factor, alpha-induced protein 2), Genebank (Genebank) NM_006290 (Tumor necrosis factor, alpha-induced protein 3), Genebank (Genebank) NM_007115 (Tumor necrosis factor, alpha-induced protein 6), Genebank (Genebank) BC052977 (Tumor necrosis factor receptor superfamily, member 1B), Genebank Number (Genebank) NM_003811 [Tumor n ecrosis factor (ligand) superfamily, member 9], Genebank AB210011 [Transportin 2 (importin 3, karyopherin beta 2b)], Genebank NM_005427 (Tumor protein p73), Genebank (Genebank) AB032995 (Two pore segment channel 1), Genebank S76980 [hexosaminidase A (alpha polypeptide)], Genebank NM_003320 [Tubby homolog (mouse)], Genebank number NM_003328 (TXK tyrosine kinase, Genebank NM_006472 (Thioredoxin interacting protein), Genebank NG_003360 [UDP glycosyltransferase 8 (UDP-galactose ceramide galactosyltransferase)], Genebank NM_025154 [Unc-84 homolog A ( C.  elegans)], Genebank BC094797 [Unc-84 homolog B (C.  elegans)], Genebank AW016806 (Transcribed locus), Genebank A (Genebank) AW341701 (Transcribed locus), Genebank (Genebank) NM_199511 (Steroid sensitive gene 1), Genebank (Genebank) DQ097177 ( HECT, UBA and WWE domain containing 1), Genebank (Genebank) BC027954 (Tcell receptor gamma variable 7), Genebank (Genebank) NM_003371 (Vav 2 oncogene), Genebank (Genebank) AF167081 (Variable charge, X linked), Genebank AK058041 [Vestigial like 4 (Drosophila)], Genebank (Genebank) AJ132099 (Vanin 1), Genebank (Genebank) NM_014588 [Visual system homeobox 1 homolog, CHX10-like ( zebrafish)], Genebank NM_000638 [Vitronectin (serum spreading factor, somatomedin B, complement S-protein)], Genebank NM_022170 (Myosin XVB pseudogene), Genebank NM_032951 (MLX) interacting protein-like, Genebank BC 052234 (Wingless-type MMTV integration site family, member 10A), Genebank AK126706 (X-ray repair complementing defective repair in Chinese hamster cells 3), Genebank NM_020205 (Zinc finger, A20 domain containing 1), Genebank D26122 (splicing factor 1), Genebank NM_032441 (Zinc finger, matrin type 1), Genebank BC036823 (Zinc finger protein 289, ID1 regulated), gene Genebank NM_182975 (Zinc finger protein 326), Genebank NM_181489 (Zinc finger protein 445), Genebank BG201273 [Zinc finger protein 75 (D8C6)], Genebank (Genebank) AY008294 [Zinc finger protein, subfamily 1A, 2 (Helios)].

본 발명자들은 폐독성 유발 약물 검색용 마커유전자를 발굴하기 위하여, 폐독성을 나타내는 니트로푸란토인을 인간 기관지 상피 세포주(BEAS-2B)에 처리하여 세포 독성을 확인하였다. 그 결과, 상기 니트로푸란토인은 인간 기관지 상피 세포주에 독성을 가짐이 확인되었고(도 1 참조), 상기 실험을 바탕으로 니트로푸란토인의 처리를 위한 농도를 결정하였다. 이후 상기 결정된 농도로 니트로푸란토인을 인간 기관지 상피 세포주에 처리하고, 상기 약물을 처리한 세포주에서 mRNA를 분리하여 cDNA를 합성하면서 Cy5 형광물질로 표지하였으며, 약물을 처리하지 않은 대조군의 경우 Cy3 형광물질로 표지하였다. 상기 형광 표지된 cDNA를 44 k 인간 유전체 전체(Human whole genome) 올리고마이크로어레이 칩(Agilent, USA)과 혼성화하였고, 형광 이미지를 스캔하여 유전자 발현 양상의 차이를 분석하였다(도 2 참조). 분석시 Cy5/Cy3 비율이 2.0 배 이상인 경우 발현 증가된 유전자로 분류하였고, 0.5 배 이하인 경우 발현 감소화된 유전자로 분류하였다. 그 결과, 발현이 증가된 유전자는 0.375%(44,290개의 유전자 중 166개), 발현이 감소된 유전자는 0.921%(44,290개의 유전자 중 408개)임을 확인하였다. 이때, 니트로푸란토인에 의해 2.0 배 이상 발현이 변화된 유전자들을 기능별로 분류하였을 때, 폐독성에 작용하는 기능으로 판단되는 세포자살(apoptosis), 전사(transcription), 면역반응(immune response), 이온수송(ion transport), 세포주기(cell cycle), 단백질 분해(proteolysis), DNA 대사(DNA metabolism), 해당과정(glycolysis), 혈관신생(angiogenesis), TGF-β, MAPK 또는 JAK-STAT에 관련된 유전자를 선별하였다(표 2 및 표 3 참조). 상기 유전자들은 본 발명에서 사용한 니트로푸란토인을 처리했을 때, 인간 기관지 상피 세포에서 독성과 관련이 있다는 보고는 없다. In order to discover marker genes for pulmonary toxicity-induced drug search, the present inventors treated cytokines with human bronchial epithelial cell lines (BEAS-2B) that exhibited lung toxicity. As a result, the nitrofurantoin was confirmed to be toxic to the human bronchial epithelial cell line (see FIG. 1), and the concentration for the treatment of nitrofurantoin was determined based on the experiment. Then, nitrofurantoin was treated to human bronchial epithelial cell line at the determined concentration, mRNA was isolated from the drug-treated cell line, and cDNA was synthesized and labeled with Cy5 fluorescent material. Was labeled. The fluorescently labeled cDNA was hybridized with a 44k human whole genome oligomicroarray chip (Agilent, USA), and fluorescent images were scanned to analyze differences in gene expression patterns (see FIG. 2). In the analysis, when the Cy5 / Cy3 ratio was 2.0 times or more, it was classified as an expression increased gene. As a result, it was confirmed that the expression of the increased gene was 0.375% (166 of 44,290 genes), 0.99% of the reduced expression (408 of 44,290 genes). At this time, when the genes whose expression is changed by 2.0 times or more by nitrofurantoin are classified by function, apoptosis, transcription, immune response, and ion transport, which are judged to function in pulmonary toxicity, are identified. genes involved in ion transport, cell cycle, proteolysis, DNA metabolism, glycolysis, angiogenesis, TGF-β, MAPK, or JAK-STAT Screened (see Table 2 and Table 3). The genes have not been reported to be associated with toxicity in human bronchial epithelial cells when treated with nitrofurantoin used in the present invention.

본 발명의 실시예에서는 상기 유전자 중 JAk-STAT 관련 유전자 7종, TGF-β 관련 유전자 3종, 및 MAPK(mitogen-activated protein kinase) 관련 유전자 8종을 분리하여 실시간 역전사유전자증폭기술(real-time reverse transcript polymerase chain reaction, RT-PCR) 방법으로 발현 양상을 조사하였다. 그 결과, 4종의 과발 현 유전자들과 14종의 저발현 유전자의 발현 양상이 올리고 마이크로어레이 칩 결과와 유사하게 나타남을 확인하였다(표 5 참조).In an embodiment of the present invention, the JAk-STAT related gene, seven TGF-β related genes, and eight kinds of mitogen-activated protein kinase (MAPK) related genes are separated and real-time reverse transcription amplification technology (real-time) Reverse transcript polymerase chain reaction (RT-PCR) was used to investigate the expression pattern. As a result, it was confirmed that the expression patterns of four overexpression genes and 14 low expression genes were similar to those of the oligo microarray chip (see Table 5).

또한, 본 발명은 상기 마커유전자 유전자 서열의 전부 또는 일부를 포함하는 올리고 뉴클레오티드, 또는 이의 상보가닥 분자가 집적된 폐독성 유발 약물 검색용 DNA 마이크로어레이 칩을 제공한다.In addition, the present invention provides a DNA microarray chip for pulmonary toxicity-induced drug search in which an oligonucleotide, or a complementary strand molecule thereof, including all or a portion of the marker gene gene sequence is integrated.

상기 올리고 뉴클레오티드 또는 이의 상보가닥 분자는 상기 마커유전자의 18 내지 30개의 핵산을 포함하고, 바람직하게는 20 내지 25개의 핵산을 포함한다. The oligonucleotide or complementary strand molecule thereof comprises 18 to 30 nucleic acids of the marker gene, preferably 20 to 25 nucleic acids.

본 발명의 폐독성 유발 약물 검색용 DNA 마이크로어레이 칩은 당업자에게 알려진 방법으로 제작할 수 있다. 상기 마이크로어레이칩을 제작하는 방법은 하기와 같다:The DNA microarray chip for pulmonary toxicity-inducing drug screening of the present invention can be produced by methods known to those skilled in the art. The method of fabricating the microarray chip is as follows:

상기 탐색된 마커유전자를 탐침 DNA 분자로 이용하여 DNA 칩의 기판상에 고정화시키기 위해 파이조일렉트릭(piezoelectric) 방식을 이용한 마이크로피펫팅(micropipetting)법 또는 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용하는 것이 바람직하나 이에 한정되는 것은 아니다. 본 발명의 바람직한 실시예에서는 핀 형태의 스폿터인 마이크로어레이를 이용하였다. 상기 DNA 마이크로어레이 칩의 기판은 아미노-실란(amino-silane), 폴리-L-라이신(poly-L-lysine) 및 알데히드(aldehyde)로 이루어진 군에서 선택되는 하나의 활성기가 코팅된 것이 바람직하나 이에 한정하는 것은 아니다. 또한, 상기 기판은 슬라이드 글래스, 플라스틱, 금속, 실리콘, 나일론 막 및 니트로셀룰로스 막(nitrocellulose membrane)으 로 이루어진 군에서 선택되는 어느 하나를 사용할 수 있으나 이에 한정되는 것은 아니다. 본 발명의 바람직한 실시예에서는 아미노-실란이 코팅된 슬라이드 글래스를 이용하였다. In order to immobilize the detected marker gene as a probe DNA molecule on a substrate of a DNA chip, a micropipetting method or a pin-shaped spotter using a piezoelectric method is used. It is preferable to use the method used, but is not limited thereto. In a preferred embodiment of the present invention, a microarray, which is a pin-shaped spotter, is used. The substrate of the DNA microarray chip is preferably coated with one active group selected from the group consisting of amino-silane, poly-L-lysine, and aldehyde. It is not limited. In addition, the substrate may be any one selected from the group consisting of slide glass, plastic, metal, silicon, nylon membrane, and nitrocellulose membrane, but is not limited thereto. In a preferred embodiment of the present invention, amino-silane-coated slide glass was used.

또한, 본 발명은 상기 마커유전자를 이용한 폐독성 유발 약물 검색 방법을 제공한다.The present invention also provides a method for screening for pulmonary toxicity-inducing drugs using the marker gene.

본 발명은The present invention

1) 인간 기관지 세포 또는 폐 세포에 피검화합물을 처리하는 단계; 1) treating the test compound with human bronchial cells or lung cells;

2) 단계 1)의 피검 화합물을 처리한 실험군 세포와 피검화합물을 처리하지 않은 대조군 세포에서 RNA를 분리하는 단계; 2) separating RNA from the experimental group cells treated with the test compound of step 1) and control cells not treated with the test compound;

3) 단계 2)의 실험군과 대조군의 RNA를 cDNA로 합성하면서 실험군과 대조군을 각기 다른 형광물질을 표지하는 단계; 3) synthesizing RNA of the experimental group and the control group of step 2) with cDNA and labeling the fluorescent substance of the experimental group and the control group, respectively;

4) 단계 3)의 각기 다른 형광물질로 표지된 cDNA를 DNA 마이크로어레이칩과 혼성화시키는 단계; 4) hybridizing cDNA labeled with different fluorescent materials of step 3) with DNA microarray chips;

5) 단계 4)의 반응한 DNA 마이크로어레이칩을 분석하는 단계; 및 5) analyzing the reacted DNA microarray chip of step 4); And

6) 단계 5)의 분석한 데이터에서 본 발명의 마커유전자의 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는 폐독성 유발 약물 검색 방법을 제공한다.6) provides a pulmonary toxicity-inducing drug search method comprising the step of confirming the expression level of the marker gene of the present invention in the analyzed data of step 5) compared to the control.

상기 방법에 있어서, 단계 1)의 인간 기관지 세포는 인간 기관지 상피 세포인 BEAS-2B를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간 기관지 또는 폐로부터 유래한 세포라면 모두 사용 가능하다.In the above method, the human bronchial cells of step 1) preferably use BEAS-2B, which is a human bronchial epithelial cell.

상기 방법에 있어서, 단계 3)의 형광물질은 Cy3, Cy5, 폴리 L-라이신-플루오레세인 이소티오시아네이트(poly L-lysine-fluorescein isothiocyanate, FITC), 로다민-B-이소티오시아네이트(rhodamine-B-isothiocyanate, RITC), 로다민(rhodamine)으로 이루어진 군으로부터 선택된 어느 하나인 것이 바람직하나 이에 한정하는 것은 아니며, 당업자에게 알려진 형광물질은 모두 사용 가능하다.In the method, the fluorescent material of step 3) is Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate ( rhodamine-B-isothiocyanate (RITC), rhodamine (rhodamine) is preferably any one selected from the group consisting of, but not limited to, any fluorescent material known to those skilled in the art can be used.

상기 방법에 있어서, 단계 5)의 DNA 마이크로어레이 칩은 Whole Human Genome Oligo Microarray(Agilent, USA)을 사용하는 것이 바람직하나, 이에 한정하는 것은 아니며, 인간 게놈 중 본 발명에서 상기 공통적으로 과발현 또는 저발현 유전자(표 2 및 표 3 참조)가 탑재된 마이크로어레이 칩이라면 모두 사용 가능하며 본 발명의 실시예에서는 직접 제작한 DNA 마이크로어레이 칩을 사용하였다. 또한, 단계 5)의 분석 방법은 GenePix 4.1 소프트웨어(Axon Instruments, USA)를 사용하는 것이 바람직하나 이에 한정하는 것은 아니며, 당업자에게 알려진 분석 소프트웨어라면 모두 사용 가능하다.In the above method, the DNA microarray chip of step 5) preferably uses Whole Human Genome Oligo Microarray (Agilent, USA), but is not limited thereto. Any microarray chip loaded with genes (see Tables 2 and 3) can be used. In the embodiment of the present invention, a DNA microarray chip manufactured directly was used. In addition, the analysis method of step 5) preferably uses GenePix 4.1 software (Axon Instruments, USA), but is not limited thereto. Any analysis software known to those skilled in the art may be used.

또한, 본 발명은In addition, the present invention

1) 인간 기관지 세포 또는 폐 세포에 피검화합물을 처리하는 단계; 1) treating the test compound with human bronchial cells or lung cells;

2) 단계 1)의 피검화합물을 처리한 실험군 세포와 피검화합물을 처리하지 않은 대조군 세포에서 RNA를 분리하는 단계; 2) separating RNA from the test cell treated with the test compound of step 1) and the control cell not treated with the test compound;

3) 단계 2)의 RNA를, 본 발명의 마커유전자에 상보적이고 마커유전자 유전자를 증폭할 수 있는 프라이머를 사용하여 실시간 RT-PCR을 수행하는 단계; 및 3) performing real-time RT-PCR using the RNA of step 2) using a primer complementary to the marker gene of the present invention and capable of amplifying the marker gene; And

4) 단계 3)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는 폐독성 유발 약물 검색 방법을 제공한다.4) provides a method for screening for pulmonary toxicity-inducing drugs comprising comparing the gene product of step 3) with a control to confirm the expression level.

상기 방법에 있어서, 단계 3)의 프라이머는 본 발명에서 탐색된 마커유전자 유전자와 상보적이고, 마커유전자를 증폭할 수 있으며, 증폭산물이 100 내지 300 bp(base pair)가 되도록 설계된 프라이머라면 모두 사용가능하다. 본 발명에서는 서열번호 1 내지 36으로 기재되는 정방향 및 역방향 프라이머 18쌍을 제시하였으나 이에 한정하는 것은 아니다.In the above method, the primer of step 3) is complementary to the marker gene gene searched for in the present invention, can amplify the marker gene, and can be used as long as the primer is designed so that the amplification product is 100 to 300 bp (base pair). Do. In the present invention, 18 pairs of forward and reverse primers set forth in SEQ ID NOS: 1 to 36 are provided, but the present invention is not limited thereto.

아울러, 본 발명은 상기 DNA 마이크로어레이 칩을 포함하는 폐독성 유발 약물 검색용 키트를 제공한다.In addition, the present invention provides a kit for pulmonary toxicity-induced drug search comprising the DNA microarray chip.

상기 DNA 마이크로어레이 칩은 본 발명의 방법으로 제작한 것을 사용하는 것이 바람직하나 이에 한정하지 않는다.The DNA microarray chip is preferably used by the method of the present invention, but is not limited thereto.

상기 검색용 키트는 추가적으로 인간 기관지 상피 세포를 포함할 수 있으며, 인간 기관지 상피 세포는 BEAS-2B를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간 기관지 또는 폐로부터 유래한 세포라면 모두 사용 가능하다.The search kit may additionally include human bronchial epithelial cells, and the human bronchial epithelial cells may preferably be BEAS-2B, but are not limited thereto. Any human bronchial or lung cells may be used.

상기 검색용 키트는 추가적으로 형광물질을 포함할 수 있으며, 형광물질은 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin-like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 군으로부터 선택되는 것이 바람직하나 이에 한정되는 것은 아니며, 본 발명의 바람직한 실시예에서는 Cy3와 Cy5를 사용하였다. The search kit may further include a fluorescent material, and the fluorescent material may be composed of a streptadin-like phosphatease conjugate, a chemical fluorescence (chemiflurorensce), and a chemiluminescent material (chemiluminescent). Preferably, but not limited to selected from the group, in the preferred embodiment of the present invention used Cy3 and Cy5.

상기 검색용 키트에 추가적으로 반응 시약을 포함할 수 있으며, 반응 시약은 혼성화에 사용되는 완충용액, RNA로부터 cDNA를 합성하기 위한 역전사효소, cNTPs 및 rNTP(사전 혼합형 또는 분리 공급형), 형광 염색제의 화학적 유도제와 같은 표식시약, 세척 완충용액 등으로 구성될 수 있으나 이에 한정된 것은 아니며, 당업자에게 알려진 DNA 마이크로어레이 칩의 혼성화 반응에 필요한 반응 시약은 모두 포함할 수 있다.The detection kit may further include a reaction reagent, and the reaction reagent may be a buffer solution used for hybridization, reverse transcriptase for synthesizing cDNA from RNA, cNTPs and rNTP (premixed or separated feed), and chemical fluorescence staining agents. It may be composed of a labeling reagent such as an inducing agent, a washing buffer, and the like, but is not limited thereto, and may include all reaction reagents required for hybridization of DNA microarray chips known to those skilled in the art.

상기 검색용 키트에 추가적으로 상기 마커유전자 유전자에 상보적이고, 마커유전자 유전자를 증폭할 수 있는 프라이머를 포함할 수 있으며, 프라이머는 서열번호 1 내지 36로 기재되는 서열로 구성된 군으로부터 선택되는 어느 하나의 프라이머를 사용하는 것이 바람직하나, 이에 한정되는 것은 아니며, 상기 마커유전자에 상보적이고, 마커유전자 유전자를 증폭할 수 있으며 증폭산물이 100 내지 300bp가 되도록 설계된 정방향 또는 역방향 프라이머쌍은 모두 사용 가능하다.The kit may further include a primer complementary to the marker gene gene and capable of amplifying the marker gene gene, wherein the primer is any one primer selected from the group consisting of SEQ ID NOs: 1 to 36. It is preferable to use, but is not limited thereto. Complementary to the marker gene, and can be amplified marker gene gene, all the forward or reverse primer pairs designed to be 100 to 300bp can be used.

이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of examples.

단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

<< 실시예Example 1> 세포 배양 및 화학물질 처리 1> Cell Culture and Chemical Treatment

<1-1> 세포배양<1-1> Cell Culture

인간 기관지 상피 세포주인 BEAS-2B 세포(CRL-9609, ATCC, USA)를 10% FBS가 첨가된 DMEM 배지(Gibro-BRL, USA)를 이용하여 T 75 플라스크에서 5 × 105 세포/ml가 되도록 배양하였다. 본 발명자들은 기존의 연구와 보고를 통해 폐독성을 부작용으로 나타나는 대표적인 약물인 니트로푸란토인을 선정하였으며, DMSO에 용해시켰다. 매질(vehicle) 농도는 모든 실험에서 0.1% 이하였다.BEAS-2B cells (CRL-9609, ATCC, USA), a human bronchial epithelial cell line, were allowed to reach 5 × 10 5 cells / ml in T 75 flasks using DMEM medium (Gibro-BRL, USA) with 10% FBS. Incubated. The present inventors have selected nitrofurantoin, a representative drug that shows lung toxicity as a side effect through existing studies and reports, and dissolved in DMSO. Vehicle concentration was less than 0.1% in all experiments.

<1-2> 세포 독성 실험(<1-2> cytotoxicity test ( MTTMTT assayassay ) 및 화학 물질 처리) And chemical processing

Mossman 등(J. Immunol . Methods, 65, 55-63, 1983)의 방법으로 BEAS-2B 세포주를 이용한 MTT 실험을 수행하였다. 세포는 24-웰 플레이트에 3× 104/웰 세포수로 DMEM 배지(Gibro-BRL, USA)에서 DMSO에 용해된 니트로푸란토인을 처리하고 48시간 후에 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetra zolium bromide) 4 ㎎/㎖을 혼합하여 튜브에 가하여 37℃에서 3 시간 동안 배양하였다. 이 후 배지를 제거하고 형성된 포르마잔 크리스탈(formazan crystal)을 DMSO 500 ㎕에 용해하였다. 96-웰 플레이트로 옮겨 분주(aliquot)하고 흡광도 540 nm에서 O.D.값을 측정하였다. BEAS-2B 세포주에서 니트로푸란토인의 세포독성을 살펴본 결과, 20% 생존 저해율을 보이는 농도(IC20)는 89.45 uM 이었으며(도 1 참조), 상기 농도로 결정하여 마이크로어레이 실험을 수행하였다. MTT experiment using BEAS-2B cell line was performed by the method of Mossman et al . ( J. Immunol . Methods , 65, 55-63, 1983). Cells were treated with nitrofurantoin dissolved in DMSO in DMEM medium (Gibro-BRL, USA) with 3 × 10 4 / well cell counts in 24-well plates and after 48 h MTT (3- [4,5-dimethylthiazol- 2-yl] -2,5-diphenyltetra zolium bromide) 4 mg / ml was added to the tube and incubated at 37 ° C. for 3 hours. Thereafter, the medium was removed and the formed formazan crystal was dissolved in 500 µl of DMSO. Transfer to a 96-well plate was aliquoted and the OD value was measured at absorbance 540 nm. As a result of examining the cytotoxicity of nitrofurantoin in the BEAS-2B cell line, the concentration showing 20% survival inhibition (IC 20 ) was 89.45 uM (see FIG. 1), and the microarray experiment was performed by determining the concentration.

<< 실시예Example 2>  2> 마이크로어레이Microarray 실험 Experiment

<2-1> 표적 <2-1> target RNARNA 의 분리 및 형광 물질 표지Isolation and Labeling of Fluorescent Materials

1.24 × 106 cell/㎖ 농도로 100 mm 디쉬에 BEAS-2B 세포를 분주한 후, 실시예<1-2>에서 결정된 니트로푸란토인의 농도를 48 시간 동안 처리하였다. 이후, 상기 처리한 세포에서 트리졸(trizol) 시약(Invitrogen life technologies, USA)을 사용하여 제조사의 방법대로 전체 RNA를 분리하고, RNeasy mini kit(Qiagen, USA)를 사용하여 정제하였다. 게놈 DNA는 RNA 정제 동안 RNase-free DNase set(Qiagen, USA)를 사용하여 제거하였다. 각 전체 RNA 시료의 양은 분광광도계로 측정하였고, 순도는 Agilent 2100 Bioanalyzer(Agilent Technologies, USA)으로 확인하였다. After dispensing BEAS-2B cells in a 100 mm dish at a concentration of 1.24 × 10 6 cells / ml, the concentration of nitrofurantoin determined in Example <1-2> was treated for 48 hours. Thereafter, the whole cells were separated from the treated cells using a trizol reagent (Invitrogen life technologies, USA) according to the manufacturer's method, and purified using the RNeasy mini kit (Qiagen, USA). Genomic DNA was removed using RNase-free DNase set (Qiagen, USA) during RNA purification. The amount of each total RNA sample was measured by spectrophotometer, and the purity was confirmed by Agilent 2100 Bioanalyzer (Agilent Technologies, USA).

<2-2> <2-2> 표지된Labeled cDNAcDNA 제조  Produce

올리고 마이크로어레이 분석을 위하여 실시예<2-1>에서 수득한 니트로푸란토인 처리군의 전체 RNA를 사용하여 cDNA를 제조하였다. 상기 수득한 전체 RNA 30 ㎍과 올리고(dT) 프라이머 2 ㎍(1 ㎍/㎕)과 혼합하고 65℃에서 10분간 반응시킨 후 바로 얼음에 넣어 어닐링(annealing)시켰다. 상기 어닐링된 RNA의 역전사(Reverse Transcript) 반응을 위해 표 1과 같이 시약을 혼합하였다. CDNA was prepared using total RNA of the nitrofurantoin treated groups obtained in Example <2-1> for oligo microarray analysis. The total RNA obtained above was mixed with 30 μg of oligo (dT) primer and 2 μg (1 μg / μl), reacted at 65 ° C. for 10 minutes, and then annealed in ice. Reagents were mixed as shown in Table 1 for Reverse Transcript reaction of the annealed RNA.

구성Configuration 부피(㎕)Volume (μl) 5X first strand buffer5X first strand buffer 66 dNTPsdNTPs 0.60.6 0.1 M DDT0.1 M DDT 33 SuperScript II enzymeSuperScript II enzyme 33 Cy-3 or Cy-5 dUTPCy-3 or Cy-5 dUTP 22

대조군인 BEAS-2B 세포주에서 분리한 전체 RNA는 Cy3-dUTP(녹색)으로 표지화하였고, 니트로푸란토인을 처리한 BEAS-2B 세포주로부터 분리한 RNA는 Cy5-dUTP(적색)를 표지화하였다. 이때 두 시료는 Microcon YM-30 컬럼(Millipore, USA)을 사용하여 혼합, 정제되었다. Total RNA isolated from the control BEAS-2B cell line was labeled with Cy3-dUTP (green), while RNA isolated from nitrofurantoin-treated BEAS-2B cell line was labeled with Cy5-dUTP (red). At this time, the two samples were mixed and purified using a Microcon YM-30 column (Millipore, USA).

<2-3> <2-3> 혼성화Hybridization 반응 reaction

혼성화 및 세척 과정은 지노첵(주)의 지시방법에 따라 수행되었다. 혼성화는 12시간 동안 62℃ 오븐에서 수행되었다. DNA 마이크로어레이 칩으로 44 k Whole Human Genome 올리고 마이크로어레이(Agilent, USA)를 이용하였다. 세척(2분간 2× SSC/0.1% SDS에 세척, 3분간 1× SSC, 2분간 0.2× SSC에 세척) 후 슬라이드는 3분간 800 rpm으로 원심분리하여 건조하였다. Hybridization and washing procedures were carried out according to the instructions of Genochem Co., Ltd. Hybridization was performed in a 62 ° C. oven for 12 hours. A 44 k Whole Human Genome oligo microarray (Agilent, USA) was used as the DNA microarray chip. After washing (washing in 2 × SSC / 0.1% SDS for 2 minutes, washing in 1 × SSC for 3 minutes, 0.2 × SSC for 2 minutes), the slides were dried by centrifugation at 800 rpm for 3 minutes.

<2-4> 형광 이미지 획득<2-4> Fluorescence Image Acquisition

슬라이드 상의 혼성화 이미지는 Genepix 4000B(Axon Instruments, USA)로 스캔하였다. 결합하지 않은 유전자를 세척한 칩은 레이저 광 스캐너(laser fluorescence scanner)를 사용하여 형광 이미지를 획득하였다. 이때 녹색 형광 이미지는 대조군에서, 적색 형광 이미지는 실험군에서만 특이하게 발현되는 유전자의 활성정도를 나타내게 되며, 노란색 형광 이미지는 녹색과 적색의 보색으로 두 군의 발현이 큰 차이가 없음을 의미한다. 스캔한 이미지들은 유전자 발현 비율을 얻기 위하여 GenePix 4.1 소프트웨어(Axon Instruments, USA)로 분석하였다. 이렇게 얻어진 데이터로부터 니트로푸란토인에 대한 마커 유전자를 선별하였다(도 2 참조). Hybridization images on the slides were scanned with the Genepix 4000B (Axon Instruments, USA). Chips washed with unbound genes were obtained with a fluorescence image using a laser fluorescence scanner. In this case, the green fluorescence image in the control group, the red fluorescence image represents the activity level of the gene specifically expressed only in the experimental group, and the yellow fluorescence image is the complementary color of green and red, which means that there is no significant difference between the two groups. Scanned images were analyzed with GenePix 4.1 software (Axon Instruments, USA) to obtain gene expression rates. Marker genes for nitrofurantoin were selected from the data thus obtained (see FIG. 2).

그 결과, 올리고 칩 상에 존재하는 대략 4만 4천 개의 유전자 중에서 니트로푸란토인에 의해 Cy5/Cy3의 비율이 2.0배 이상으로 유전자 발현 증가를 보이는 유전자는 0.375%(44,290개의 유전자 중 166개)이고, 발현이 감소된 유전자는 0.921%(44,290개의 유전자 중 408개)임을 확인하였다. As a result, among the approximately 44,000 genes present on the oligo chip, the ratio of Cy5 / Cy3 increased by 2.0 times or more by nitrofurantoin was 0.375% (166 of 44,290 genes). , The reduced expression gene was 0.921% (408 out of 44,290 genes) was confirmed.

이때, 니트로푸란토인에 의해 2.0 배 이상 발현이 변화된 유전자들을 기능별로 분류하였을 때, 폐독성에 작용하는 기능으로 판단되는 세포자살(apoptosis), 전사(transcription), 면역반응(immune response), 이온수송(ion transport), 세포주기(cell cycle), 단백질 분해(proteolysis), DNA 대사(DNA metabolism), 해당과정(glycolysis), 혈관신생(angiogenesis), TGF-β, MAPK 또는 JAK-STAT과 관련된 유전자를 선별하였다(표 2 및 표 3 참조). 상기 유전자들은 본 발명에서 사용한 니트로푸란토인을 처리했을 때, 인간 기관지 상피 세포에서 독성과 관련이 있다는 보고는 없다. At this time, when the genes whose expression is changed by 2.0 times or more by nitrofurantoin are classified by function, apoptosis, transcription, immune response, and ion transport, which are judged to function in pulmonary toxicity, are identified. genes related to ion transport, cell cycle, proteolysis, DNA metabolism, glycolysis, angiogenesis, TGF-β, MAPK, or JAK-STAT Screened (see Table 2 and Table 3). The genes have not been reported to be associated with toxicity in human bronchial epithelial cells when treated with nitrofurantoin used in the present invention.

니트로푸란토인에To nitrofurantoin 의해 발현이 증가하는 유전자 Genes with increased expression 등록번호Registration Number 유전자 약어Gene abbreviation 유전자 명Gene name 중간값의 비Ratio of medians 1. JAK-STATJAK-STAT NM_053056NM_053056 CCND1CCND1 Cyclin D1Cyclin D1 2.11372.1137 BC069137BC069137 IL5IL5 Interleukin 5(colony-stimulating factor, eosinophil)Interleukin 5 (colony-stimulating factor, eosinophil) 2.03452.0345 2. MAPK2. MAPK NM_006597NM_006597 HSPA8HSPA8 Heat shock 70kDa protein 8Heat shock 70kDa protein 8 2.20482.2048 AK128769AK128769 CACNLB2CACNLB2 calcium channel, voltage-dependent, beta 2 subunitcalcium channel, voltage-dependent, beta 2 subunit 2.19982.1998 NM_004465NM_004465 FGF10FGF10 Fibroblast growth factor 10Fibroblast growth factor 10 2.0182.018 3. 세포자살(apoptosis)3. Apoptosis NM_003840NM_003840 TNFRSF10DTNFRSF10D Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domainTumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain 2.02662.0266 AY154467AY154467 NALP12NALP12 NACHT, leucine rich repeat and PYD containing 12NACHT, leucine rich repeat and PYD containing 12 2.052.05 NM_004323NM_004323 BAG1BAG1 BCL2-associated athanogeneBCL2-associated athanogene 2.03782.0378 AF055634AF055634 UNC5CUNC5C Unc-5 homolog C(C. elegans)Unc-5 homolog C (C. elegans) 2.32972.3297 NM_005345NM_005345 HSPA1AHSPA1A Heat shock 70kDa protein 1AHeat shock 70kDa protein 1A 2.12142.1214 AB209361AB209361 FASFAS Fas(TNF receptor superfamily, member 6)Fas (TNF receptor superfamily, member 6) 2.43872.4387 NM_004040NM_004040 RHOBRHOB Ras homolog gene family, member BRas homolog gene family, member B 2.51582.5158 BC041022BC041022 SCAND1SCAND1 SCAN domain containing 1SCAN domain containing 1 2.3942.394 4. 전사(transcription)4. Transcription BC026316BC026316 ST18ST18 Suppression of tumorigenicity 18(breast carcinoma)(zinc finger protein)Suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) 2.12752.1275 NM_003662NM_003662 PIRPIR Pirin(iron-binding nuclear protein)Pirin (iron-binding nuclear protein) 2.66522.6652 BF337308BF337308 HOPHOP Homeodomain-only proteinHomeodomain-only protein 2.10652.1065 AK223249AK223249 HOXB7HOXB7 Homeo box B7Homeo box B7 2.07252.0725 NM_003670NM_003670 BHLHB2BHLHB2 Basic helix-loop-helix domain containing, class B, 2Basic helix-loop-helix domain containing, class B, 2 2.51642.5164 BC044311BC044311 LOC339344LOC339344 Hypothetical protein LOC339344Hypothetical protein LOC339344 2.16742.1674 BC053934BC053934 SIRT4SIRT4 Sirtuin(silent mating type information regulation 2 homolog) 4(S. cerevisiae)Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) 2.01692.0169 BC018064BC018064 ATF6ATF6 Activating transcription factor 6Activating transcription factor 6 2.1772.177 BC041022BC041022 SCAND1SCAND1 SCAN domain containing 1SCAN domain containing 1 2.3942.394 AF219946AF219946 TULP4TULP4 Tubby like protein 4Tubby like protein 4 2.23162.2316 NM_004465NM_004465 FGF10FGF10 Fibroblast growth factor 10Fibroblast growth factor 10 2.0182.018 AK123051AK123051 MYCBPMYCBP C-myc binding proteinC-myc binding protein 2.01132.0113 NM_033400NM_033400 ZFHX2ZFHX2 Zinc finger homeobox 2Zinc finger homeobox 2 2.03242.0324 XM_040592XM_040592 ZNF469ZNF469 Zinc finger protein 469Zinc finger protein 469 2.53832.5383 5. 면역반응(5. Immune response immuneimmune responseresponse )) BM905227BM905227 FTH1FTH1 Ferritin, heavy polypeptide 1Ferritin, heavy polypeptide 1 2.00712.0071 NM_001295NM_001295 CCR1CCR1 Chemokine(C-C motif) receptor 1Chemokine (C-C motif) receptor 1 2.06682.0668 NM_134470NM_134470 IL1RAPIL1RAP Interleukin 1 receptor accessory proteinInterleukin 1 receptor accessory protein 2.08422.0842 AY154467AY154467 NALP12NALP12 NACHT, leucine rich repeat and PYD containing 12NACHT, leucine rich repeat and PYD containing 12 2.052.05 AB209361AB209361 FASFAS Fas(TNF receptor superfamily, member 6)Fas (TNF receptor superfamily, member 6) 2.43872.4387 AK090482AK090482 CXCL12CXCL12 Chemokine(C-X-C motif) ligand 12(stromal cell-derived factor 1)Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) 2.69522.6952 BC069137BC069137 IL5IL5 Interleukin 5(colony-stimulating factor, eosinophil)Interleukin 5 (colony-stimulating factor, eosinophil) 2.03452.0345 AK026467AK026467 CD22CD22 CD22 antigenCD22 antigen 2.00392.0039 BX537504BX537504 C2C2 Complement component 2Complement component 2 2.17332.1733 AF186094AF186094 IL1F5IL1F5 Interleukin 1 family, member 5(delta)Interleukin 1 family, member 5 (delta) 2.03082.0308 6. 이온수송(ion transport)6. Ion transport BM905227BM905227 FTH1FTH1 Ferritin, heavy polypeptide 1Ferritin, heavy polypeptide 1 2.00712.0071 NM_014619NM_014619 GRIK4GRIK4 Glutamate receptor, ionotropic, kainate 4Glutamate receptor, ionotropic, kainate 4 2.35132.3513 NM_001035NM_001035 RYR2RYR2 Ryanodine receptor 2(cardiac)Ryanodine receptor 2 (cardiac) 2.02422.0242 NM_004797NM_004797 ACDCACDC Adiponectin, C1Q and collagen domain containingAdiponectin, C1Q and collagen domain containing 2.09482.0948 AK025742AK025742 UCP2UCP2 Uncoupling protein 2(mitochondrial, proton carrier)Uncoupling protein 2 (mitochondrial, proton carrier) 2.54682.5468 AK128769AK128769 MYSB; CACNLB2; FLJ23743MYSB; CACNLB2; FLJ23743 calcium channel, voltage-dependent, beta 2 subunitcalcium channel, voltage-dependent, beta 2 subunit 2.19982.1998 7. 세포주기(cell cycle)7. Cell cycle NM_004465NM_004465 FGF10FGF10 Fibroblast growth factor 10Fibroblast growth factor 10 2.0182.018 NM_053056NM_053056 CCND1CCND1 Cyclin D1Cyclin D1 2.11372.1137 NM_004040NM_004040 RHOBRHOB Ras homolog gene family, member BRas homolog gene family, member B 2.51582.5158 BC041022BC041022 SCAND1SCAND1 SCAN domain containing 1SCAN domain containing 1 2.3942.394 8. 단백질 분해(proteolysis)8. Proteolysis XM_208270XM_208270 bA395L14.13bA395L14.13 Acrosin-like proteaseAcrosin-like protease 2.06512.0651 BX108307BX108307 DEFB126DEFB126 Defensin, beta 126Defensin, beta 126 2.29692.2969 NM_002900NM_002900 RBP3RBP3 Retinol binding protein 3, interstitialRetinol binding protein 3, interstitial 2.57072.5707 BX537504BX537504 C2C2 Complement component 2Complement component 2 2.17332.1733 9. DNA 대사(DNA metabolism)9. DNA metabolism BX537939BX537939 ELNELN Elastin(supravalvular aortic stenosis, Williams-Beuren syndrome)Elastin (supravalvular aortic stenosis, Williams-Beuren syndrome) 2.27222.2722 BC053934BC053934 SIRT4SIRT4 Sirtuin(silent mating type information regulation 2 homolog) 4(S. cerevisiae)Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) 2.01692.0169 10. 해당과정(glycolysis)10. Glycolysis S81916S81916 PGK1PGK1 Phosphoglycerate kinase 1Phosphoglycerate kinase 1 2.12762.1276

니트로푸란토인에To nitrofurantoin 의해 발현이 감소하는 유전자 Genes whose expression is reduced 등록번호Registration Number 유전자 약어Gene abbreviation 유전자 명Gene name 중간값의 비Ratio of medians 1. One. JAKJAK -- STATSTAT NM_003629NM_003629 PIK3R3PIK3R3 Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) 0.47270.4727 NM_003955NM_003955 SOCS3SOCS3 Suppressor of cytokine signaling 3Suppressor of cytokine signaling 3 0.47240.4724 NM_002309NM_002309 LIFLIF Hypothetical protein MGC20647Hypothetical protein MGC20647 0.41450.4145 NM_172220NM_172220 CSF3CSF3 Colony stimulating factor 3(granulocyte)Colony stimulating factor 3 (granulocyte) 0.32120.3212 AA995107AA995107 il7Ril7R Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor [Pan troglodytes]Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor [Pan troglodytes] 0.29210.2921 2. 2. TGFTGF -β-β CR618407CR618407 BMP2BMP2 Bone morphogenetic protein 2Bone morphogenetic protein 2 0.49220.4922 U68019U68019 SMAD3SMAD3 SMAD, mothers against DPP homolog 3(Drosophila)SMAD, mothers against DPP homolog 3 (Drosophila) 0.49980.4998 BM906235BM906235 ID3ID3 Inhibitor of DNA binding 3, dominant negative helix-loop-helix proteinInhibitor of DNA binding 3, dominant negative helix-loop-helix protein 0.48460.4846 3. 3. MAPKMAPK CR614015CR614015 CD14CD14 CD14 antigenCD14 antigen 0.49960.4996 BC021552BC021552 CACNG6CACNG6 Calcium channel, voltage-dependent, gamma subunit 6Calcium channel, voltage-dependent, gamma subunit 6 0.45230.4523 AK098095AK098095 BRAFBRAF V-raf murine sarcoma viral oncogene homolog B1V-raf murine sarcoma viral oncogene homolog B1 0.42250.4225 BC008678BC008678 IL1BIL1B Interleukin 1, betaInterleukin 1, beta 0.39340.3934 NM_000575NM_000575 IL1AIL1A Interleukin 1, alphaInterleukin 1, alpha 0.36160.3616 NM_000944NM_000944 PPP3CAPPP3CA Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) 0.22880.2288 4. 전사(4. Warrior ( transcriptiontranscription )) AB028450AB028450 NCOR1NCOR1 Nuclear receptor co-repressor 1Nuclear receptor co-repressor 1 0.4932120.493212 AB028978AB028978 KIAA1055KIAA1055 TBC1 domain family, member 2BTBC1 domain family, member 2B 0.3733050.373305 AB046767AB046767 TLE3TLE3 Transducin-like enhancer of split 3(E(sp1) homolog, Drosophila)Transducin-like enhancer of split 3 (E (sp1) homolog, Drosophila) 0.4541910.454191 AB209032AB209032 ATF3ATF3 Activating transcription factor 3Activating transcription factor 3 0.46270.4627 AB209404AB209404 GLIS3GLIS3 GLIS family zinc finger 3GLIS family zinc finger 3 0.4992990.499299 AF227948AF227948 HBXAPHBXAP Hepatitis B virus x associated proteinHepatitis B virus x associated protein 0.189560.18956 AK055224AK055224 NEUROD6NEUROD6 Neurogenic differentiation 6Neurogenic differentiation 6 0.3103330.310333 AK058041AK058041 VGLL4VGLL4 Vestigial like 4(Drosophila)Vestigial like 4 (Drosophila) 0.4774150.477415 AK075040AK075040 HES6HES6 Hairy and enhancer of split 6(Drosophila)Hairy and enhancer of split 6 (Drosophila) 0.2656980.265698 AK090459AK090459 NFE2L1NFE2L1 Nuclear factor(erythroid-derived 2)-like 1Nuclear factor (erythroid-derived 2) -like 1 0.4161570.416157 AK092414AK092414 MAFKMAFK V-maf musculoaponeurotic fibrosarcoma oncogene homolog K(avian)V-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) 0.3746040.374604 AK127845AK127845 GATA2GATA2 GATA binding protein 2GATA binding protein 2 0.4892480.489248 AK128256AK128256 MEF2BMEF2B MADS box transcription enhancer factor 2, polypeptide B(myocyte enhancer factor 2B)MADS box transcription enhancer factor 2, polypeptide B (myocyte enhancer factor 2B) 0.2527360.252736 AL834223AL834223 HDAC11HDAC11 Histone deacetylase 11Histone deacetylase 11 0.4041370.404137 AY008294AY008294 ZNFN1A2ZNFN1A2 Zinc finger protein, subfamily 1A, 2(Helios)Zinc finger protein, subfamily 1A, 2 (Helios) 0.466170.46617 BC016743BC016743 BRF1BRF1 BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB(S. cerevisiae)BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) 0.3917780.391778 BC017222BC017222 SQSTM1SQSTM1 Sequestosome 1Sequestosome 1 0.3535410.353541 BC028148BC028148 TNFTNF Tumor necrosis factor(TNF superfamily, member 2)Tumor necrosis factor (TNF superfamily, member 2) 0.4737840.473784 BC033580BC033580 KLF1KLF1 Kruppel-like factor 1(erythroid)Kruppel-like factor 1 (erythroid) 0.4942640.494264 BC051352BC051352 OLIG3OLIG3 Oligodendrocyte transcription factor 3Oligodendrocyte transcription factor 3 0.4193680.419368 BG201273BG201273 ZNF75ZNF75 Zinc finger protein 75(D8C6)Zinc finger protein 75 (D8C6) 0.4227080.422708 BM906235BM906235 ID3ID3 Inhibitor of DNA binding 3, dominant negative helix-loop-helix proteinInhibitor of DNA binding 3, dominant negative helix-loop-helix protein 0.4845790.484579 BX537368BX537368 ELF3ELF3 E74-like factor 3(ets domain transcription factor, epithelial-specific )E74-like factor 3 (ets domain transcription factor, epithelial-specific) 0.1348140.134814 D26122D26122 ZFM1ZFM1 splicing factor 1splicing factor 1 0.3599110.359911 NM_000545NM_000545 TCF1TCF1 Transcription factor 1, hepatic; LF-B1, hepatic nuclear factor(HNF1), albumin proximal factorTranscription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor 0.4524070.452407 NM_000937NM_000937 POLR2APOLR2A Polymerase(RNA) II(DNA directed) polypeptide A, 220kDaPolymerase (RNA) II (DNA directed) polypeptide A, 220kDa 0.3189030.318903 NM_001426NM_001426 EN1EN1 Engrailed homolog 1Engrailed homolog 1 0.469280.46928 NM_001618NM_001618 PARP1PARP1 Poly(ADP-ribose) polymerase family, member 1Poly (ADP-ribose) polymerase family, member 1 0.4260540.426054 NM_001987NM_001987 ETV6ETV6 Ets variant gene 6(TEL oncogene)Ets variant gene 6 (TEL oncogene) 0.4829840.482984 NM_003106NM_003106 SOX2SOX2 SRY(sex determining region Y)-box 2SRY (sex determining region Y) -box 2 0.4515720.451572 NM_003107NM_003107 SOX4SOX4 SRY(sex determining region Y)-box 4SRY (sex determining region Y) -box 4 0.491850.49185 NM_003482NM_003482 MLL2MLL2 Myeloid/lymphoid or mixed-lineage leukemia 2Myeloid / lymphoid or mixed-lineage leukemia 2 0.4503550.450355 NM_005427NM_005427 TP73TP73 Tumor protein p73Tumor protein p73 0.4619040.461904 NM_005461NM_005461 MAFBMAFB V-maf musculoaponeurotic fibrosarcoma oncogene homolog B(avian)V-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) 0.3281940.328194 NM_005967NM_005967 NAB2NAB2 NGFI-A binding protein 2(EGR1 binding protein 2)NGFI-A binding protein 2 (EGR1 binding protein 2) 0.3922930.392293 NM_006162NM_006162 NFATC1NFATC1 Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 0.4895250.489525 NM_006290NM_006290 TNFAIP3TNFAIP3 Tumor necrosis factor, alpha-induced protein 3Tumor necrosis factor, alpha-induced protein 3 0.4422610.442261 NM_007313NM_007313 ABL1ABL1 V-abl Abelson murine leukemia viral oncogene homolog 1V-abl Abelson murine leukemia viral oncogene homolog 1 0.249190.24919 NM_014588NM_014588 VSX1VSX1 Visual system homeobox 1 homolog, CHX10-like(zebrafish)Visual system homeobox 1 homolog, CHX10-like (zebrafish) 0.4651030.465103 NM_015267NM_015267 CUTL2CUTL2 Cut-like 2(Drosophila)Cut-like 2 (Drosophila) 0.3878680.387868 NM_015335NM_015335 THRAP2THRAP2 Thyroid hormone receptor associated protein 2Thyroid hormone receptor associated protein 2 0.4000210.400021 NM_017745NM_017745 BCORBCOR BCL6 co-repressorBCL6 co-repressor 0.4569850.456985 NM_021170NM_021170 HES4HES4 Hairy and enhancer of split 4(Drosophila)Hairy and enhancer of split 4 (Drosophila) 0.2917890.291789 NM_032199NM_032199 ARID5BARID5B AT rich interactive domain 5B(MRF1-like)AT rich interactive domain 5B (MRF1-like) 0.4661180.466118 NM_032827NM_032827 ATOH8ATOH8 Atonal homolog 8(Drosophila)Atonal homolog 8 (Drosophila) 0.2290520.229052 NM_032951NM_032951 WBSCR14WBSCR14 MLX interacting protein-likeMLX interacting protein-like 0.0687950.068795 NM_139067NM_139067 SMARCC2SMARCC2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2SWI / SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 0.3446440.344644 NM_172196NM_172196 ALFALF TFIIA-alpha/beta-like factorTFIIA-alpha / beta-like factor 0.2159990.215999 NM_178229NM_178229 IQGAP3IQGAP3 IQ motif containing GTPase activating protein 3IQ motif containing GTPase activating protein 3 0.4379370.437937 NM_181489NM_181489 ZNF445ZNF445 Zinc finger protein 445Zinc finger protein 445 0.4794970.479497 NM_182975NM_182975 ZNF326ZNF326 Zinc finger protein 326Zinc finger protein 326 0.4517420.451742 U52682U52682 IRF4IRF4 Interferon regulatory factor 4Interferon regulatory factor 4 0.4778670.477867 U68019U68019 SMAD3SMAD3 SMAD, mothers against DPP homolog 3(Drosophila)SMAD, mothers against DPP homolog 3 (Drosophila) 0.4997510.499751 X99975X99975 H.sapiens mRNA for hRTR/hGCNF protein.H.sapiens mRNA for hRTR / hGCNF protein. 0.4737760.473776 XM_379934XM_379934 KIAA1718KIAA1718 KIAA1718 proteinKIAA1718 protein 0.496170.49617 5. 면역 반응(5. Immune response immuneimmune responseresponse )) AA995107AA995107 IL-7RIL-7R Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor [Pan troglodytes]Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor [Pan troglodytes] 0.2920880.292088 AB209622AB209622 ITKITK IL2-inducible T-cell kinaseIL2-inducible T-cell kinase 0.3826310.382631 AK001872AK001872 PDCD1LG2PDCD1LG2 Programmed cell death 1 ligand 2Programmed cell death 1 ligand 2 0.4361780.436178 AK090459AK090459 NFE2L1NFE2L1 Nuclear factor(erythroid-derived 2)-like 1Nuclear factor (erythroid-derived 2) -like 1 0.4161570.416157 AK093049AK093049 SERPINA3SERPINA3 Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 0.287340.28734 AK124894AK124894 CRHR1CRHR1 Corticotropin releasing hormone receptor 1Corticotropin releasing hormone receptor 1 0.4381020.438102 BC008678BC008678 IL1BIL1B Interleukin 1, betaInterleukin 1, beta 0.3933660.393366 BC013780BC013780 ADORA2AADORA2A Adenosine A2a receptorAdenosine A2a receptor 0.4712380.471238 BC017222BC017222 SQSTM1SQSTM1 Sequestosome 1Sequestosome 1 0.3535410.353541 BC020698BC020698 CCL20CCL20 Chemokine(C-C motif) ligand 20Chemokine (C-C motif) ligand 20 0.1072760.107276 BC021117BC021117 CSF1CSF1 Colony stimulating factor 1(macrophage)Colony stimulating factor 1 (macrophage) 0.4050050.405005 BC028148BC028148 TNFTNF Tumor necrosis factor(TNF superfamily, member 2)Tumor necrosis factor (TNF superfamily, member 2) 0.4737840.473784 BC053653BC053653 CXCL2CXCL2 Chemokine(C-X-C motif) ligand 2Chemokine (C-X-C motif) ligand 2 0.4187570.418757 BC063852BC063852 C3C3 Complement component 3Complement component 3 0.4365450.436545 BC071718BC071718 CCL4L1CCL4L1 Chemokine(C-C motif) ligand 4-like 1Chemokine (C-C motif) ligand 4-like 1 0.4145650.414565 BE873976BE873976 CSF2CSF2 Colony stimulating factor 2(granulocyte-macrophage)Colony stimulating factor 2 (granulocyte-macrophage) 0.4546550.454655 BG564326BG564326 SAA4SAA4 Serum amyloid A4, constitutiveSerum amyloid A4, constitutive 0.3000040.300004 BG564669BG564669 SAA1SAA1 Serum amyloid A2Serum amyloid A2 0.3510940.351094 CR614015CR614015 CD14CD14 CD14 antigenCD14 antigen 0.4995670.499567 CR627018CR627018 MBPMBP Myelin basic proteinMyelin basic protein 0.326010.32601 NM_000575NM_000575 IL1AIL1A Interleukin 1, alphaInterleukin 1, alpha 0.3616110.361611 NM_000600NM_000600 IL6IL6 Interleukin 6(interferon, beta 2)Interleukin 6 (interferon, beta 2) 0.4245190.424519 NM_000638NM_000638 VTNVTN Vitronectin(serum spreading factor, somatomedin B, complement S-protein)Vitronectin (serum spreading factor, somatomedin B, complement S-protein) 0.4936490.493649 NM_001511NM_001511 CXCL1CXCL1 Chemokine(C-X-C motif) ligand 1(melanoma growth stimulating activity, alpha)Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) 0.4713730.471373 NM_001710NM_001710 BFBF B-factor, properdinB-factor, properdin 0.2639830.263983 NM_001733NM_001733 C1RC1R Complement component 1, r subcomponentComplement component 1, r subcomponent 0.3973340.397334 NM_002090NM_002090 CXCL3CXCL3 Chemokine(C-X-C motif) ligand 3Chemokine (C-X-C motif) ligand 3 0.3671690.367169 NM_002260NM_002260 KLRC3KLRC3 Killer cell lectin-like receptor subfamily C, member 2Killer cell lectin-like receptor subfamily C, member 2 0.2087510.208751 NM_002309NM_002309 LIFLIF Hypothetical protein MGC20647Hypothetical protein MGC20647 0.4145470.414547 NM_002341NM_002341 LTBLTB Lymphotoxin beta(TNF superfamily, member 3)Lymphotoxin beta (TNF superfamily, member 3) 0.4261390.426139 NM_002852NM_002852 PTX3PTX3 Pentraxin-related gene, rapidly induced by IL-1 betaPentraxin-related gene, rapidly induced by IL-1 beta 0.4729890.472989 NM_002982NM_002982 CCL2CCL2 Chemokine(C-C motif) ligand 2Chemokine (C-C motif) ligand 2 0.2110620.211062 NM_002994NM_002994 CXCL5CXCL5 Chemokine(C-X-C motif) ligand 5Chemokine (C-X-C motif) ligand 5 0.4325970.432597 NM_003004NM_003004 SECTM1SECTM1 Secreted and transmembrane 1Secreted and transmembrane 1 0.4649170.464917 NM_003811NM_003811 TNFSF9TNFSF9 Tumor necrosis factor(ligand) superfamily, member 9Tumor necrosis factor (ligand) superfamily, member 9 0.3569230.356923 NM_007115NM_007115 TNFAIP6TNFAIP6 Tumor necrosis factor, alpha-induced protein 6Tumor necrosis factor, alpha-induced protein 6 0.4203230.420323 NM_007329NM_007329 DMBT1DMBT1 Deleted in malignant brain tumors 1Deleted in malignant brain tumors 1 0.4738380.473838 NM_014002NM_014002 IKBKEIKBKE Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilonInhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon 0.3380140.338014 NM_015254NM_015254 KIF13BKIF13B Kinesin family member 13BKinesin family member 13B 0.464560.46456 NM_015259NM_015259 ICOSLGICOSLG Inducible T-cell co-stimulator ligandInducible T-cell co-stimulator ligand 0.1797850.179785 NM_030754NM_030754 SAA2SAA2 Serum amyloid A2Serum amyloid A2 0.283340.28334 NM_145641NM_145641 APOL3APOL3 Apolipoprotein L, 3Apolipoprotein L, 3 0.4269040.426904 NM_153443NM_153443 KIR3DL1KIR3DL1 Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 0.4644480.464448 NM_172220NM_172220 CSF3CSF3 Colony stimulating factor 3(granulocyte)Colony stimulating factor 3 (granulocyte) 0.3212430.321243 NM_212482NM_212482 FN1FN1 Fibronectin 1Fibronectin 1 0.359460.35946 U52682U52682 IRF4IRF4 Interferon regulatory factor 4Interferon regulatory factor 4 0.4778670.477867 U81234U81234 CXCL6CXCL6 Chemokine(C-X-C motif) ligand 6(granulocyte chemotactic protein 2)Chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2) 0.2797640.279764 X03663X03663 CSF1RCSF1R Colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral(v-fms) oncogene homologColony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog 0.4918880.491888 6. 이온 수송(6. Ion transport ( ionion transporttransport )) AB032995AB032995 TPCN1TPCN1 Two pore segment channel 1Two pore segment channel 1 0.4561920.456192 AF225988AF225988 SCN8ASCN8A Sodium channel, voltage gated, type VIII, alphaSodium channel, voltage gated, type VIII, alpha 0.4952970.495297 AK091926AK091926 SLC13A2SLC13A2 Solute carrier family 13(sodium-dependent dicarboxylate transporter), member 2Solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2 0.4787730.478773 AY358424AY358424 C1QTNF1C1QTNF1 C1q and tumor necrosis factor related protein 1C1q and tumor necrosis factor related protein 1 0.3672930.367293 BC021552BC021552 CACNG6CACNG6 Calcium channel, voltage-dependent, gamma subunit 6Calcium channel, voltage-dependent, gamma subunit 6 0.4523380.452338 BC098390BC098390 SLC12A7SLC12A7 Solute carrier family 12(potassium/chloride transporters), member 7Solute carrier family 12 (potassium / chloride transporters), member 7 0.3411660.341166 BX537444BX537444 ATP2B4ATP2B4 ATPase, Ca++ transporting, plasma membrane 4ATPase, Ca ++ transporting, plasma membrane 4 0.4608250.460825 NM_000393NM_000393 COL5A2COL5A2 Collagen, type V, alpha 2Collagen, type V, alpha 2 0.1391480.139148 NM_001426NM_001426 EN1EN1 Engrailed homolog 1Engrailed homolog 1 0.469280.46928 NM_001845NM_001845 COL4A1COL4A1 Collagen, type IV, alpha 1Collagen, type IV, alpha 1 0.4824670.482467 NM_001848NM_001848 COL6A1COL6A1 Collagen, type VI, alpha 1Collagen, type VI, alpha 1 0.2698690.269869 NM_001850NM_001850 COL8A1COL8A1 Collagen, type VIII, alpha 1Collagen, type VIII, alpha 1 0.3399450.339945 NM_004370NM_004370 COL12A1COL12A1 Collagen, type XII, alpha 1Collagen, type XII, alpha 1 0.4384160.438416 NM_005929NM_005929 MFI2MFI2 Antigen p97(melanoma associated) identified by monoclonal antibodies 133.2 and 96.5Antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 0.3886770.388677 NM_006045NM_006045 ATP9AATP9A ATPase, Class II, type 9AATPase, Class II, type 9A 0.4719220.471922 NM_006514NM_006514 SCN10ASCN10A Sodium channel, voltage-gated, type X, alphaSodium channel, voltage-gated, type X, alpha 0.3630170.363017 NM_033380NM_033380 COL4A5COL4A5 Collagen, type IV, alpha 5(Alport syndrome)Collagen, type IV, alpha 5 (Alport syndrome) 0.4498270.449827 NM_052892NM_052892 PKD1L2PKD1L2 Polycystic kidney disease 1-like 2Polycystic kidney disease 1-like 2 0.4016050.401605 NM_144579NM_144579 SFXN5SFXN5 Sideroflexin 5Sideroflexin 5 0.430440.43044 NM_153320NM_153320 SLC22A7SLC22A7 Solute carrier family 22(organic anion transporter), member 7Solute carrier family 22 (organic anion transporter), member 7 0.4173030.417303 NM_198056NM_198056 SCN5ASCN5A Sodium channel, voltage-gated, type V, alpha(long QT syndrome 3)Sodium channel, voltage-gated, type V, alpha (long QT syndrome 3) 0.3323430.332343 NM_203327NM_203327 SLC23A2SLC23A2 Solute carrier family 23(nucleobase transporters), member 2Solute carrier family 23 (nucleobase transporters), member 2 0.470140.47014 NM_213621NM_213621 HTR3AHTR3A 5-hydroxytryptamine(serotonin) receptor 3A5-hydroxytryptamine (serotonin) receptor 3A 0.2949010.294901 Z74615Z74615 COL1A1COL1A1 Collagen, type I, alpha 1Collagen, type I, alpha 1 0.4618260.461826 7. 세포자살(7. Apoptosis apoptosisapoptosis )) AF545571AF545571 SULF1SULF1 Sulfatase 1Sulfatase 1 0.3609790.360979 AK098095AK098095 BRAFBRAF V-raf murine sarcoma viral oncogene homolog B1V-raf murine sarcoma viral oncogene homolog B1 0.4224750.422475 BC008678BC008678 IL1BIL1B Interleukin 1, betaInterleukin 1, beta 0.3933660.393366 BC013780BC013780 ADORA2AADORA2A Adenosine A2a receptorAdenosine A2a receptor 0.4712380.471238 BC028148BC028148 TNFTNF Tumor necrosis factor(TNF superfamily, member 2)Tumor necrosis factor (TNF superfamily, member 2) 0.4737840.473784 BC052977BC052977 TNFRSF1BTNFRSF1B Tumor necrosis factor receptor superfamily, member 1BTumor necrosis factor receptor superfamily, member 1B 0.4189110.418911 BG284742BG284742 P8P8 P8 protein(candidate of metastasis 1)P8 protein (candidate of metastasis 1) 0.4664310.466431 CR614015CR614015 CD14CD14 CD14 antigenCD14 antigen 0.4995670.499567 D79987D79987 ESPL1ESPL1 Extra spindle poles like 1(S. cerevisiae)Extra spindle poles like 1 (S. cerevisiae) 0.4635690.463569 NM_000514NM_000514 GDNFGDNF Glial cell derived neurotrophic factorGlial cell derived neurotrophic factor 0.4514550.451455 NM_000575NM_000575 IL1AIL1A Interleukin 1, alphaInterleukin 1, alpha 0.3616110.361611 NM_000600NM_000600 IL6IL6 Interleukin 6(interferon, beta 2)Interleukin 6 (interferon, beta 2) 0.4245190.424519 NM_001003940NM_001003940 BMFBMF Bcl2 modifying factorBcl2 modifying factor 0.4755560.475556 NM_001165NM_001165 BIRC3BIRC3 Baculoviral IAP repeat-containing 3Baculoviral IAP repeat-containing 3 0.4516810.451681 NM_002982NM_002982 CCL2CCL2 Chemokine(C-C motif) ligand 2Chemokine (C-C motif) ligand 2 0.2110620.211062 NM_003811NM_003811 TNFSF9TNFSF9 Tumor necrosis factor(ligand) superfamily, member 9Tumor necrosis factor (ligand) superfamily, member 9 0.3569230.356923 NM_003955NM_003955 SOCS3SOCS3 Suppressor of cytokine signaling 3Suppressor of cytokine signaling 3 0.4724330.472433 NM_005427NM_005427 TP73TP73 Tumor protein p73Tumor protein p73 0.4619040.461904 NM_006290NM_006290 TNFAIP3TNFAIP3 Tumor necrosis factor, alpha-induced protein 3Tumor necrosis factor, alpha-induced protein 3 0.4422610.442261 NM_007313NM_007313 ABL1ABL1 V-abl Abelson murine leukemia viral oncogene homolog 1V-abl Abelson murine leukemia viral oncogene homolog 1 0.249190.24919 NM_139314NM_139314 ANGPTL4ANGPTL4 Angiopoietin-like 4Angiopoietin-like 4 0.4374590.437459 NM_147780NM_147780 CTSBCTSB Cathepsin BCathepsin b 0.4760260.476026 8. 혈관신생(8. Angiogenesis angiogenesisangiogenesis )) AK024680AK024680 CDNA: FLJ21027 fis, clone CAE07110CDNA: FLJ21027 fis, clone CAE07110 0.4959560.495956 AL833606AL833606 NRP2NRP2 Neuropilin 2Neuropilin 2 0.4211260.421126 NM_002825NM_002825 PTNPTN Pleiotrophin(heparin binding growth factor 8, neurite growth-promoting factor 1)Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) 0.4878190.487819 NM_003873NM_003873 NRP1NRP1 Neuropilin 1Neuropilin 1 0.4350390.435039 NM_006291NM_006291 TNFAIP2TNFAIP2 Tumor necrosis factor, alpha-induced protein 2Tumor necrosis factor, alpha-induced protein 2 0.4849150.484915 U38320U38320 MMP19MMP19 Matrix metallopeptidase 19Matrix metallopeptidase 19 0.4776950.477695

<< 실시예Example 3> 실시간  3> Real time RTRT -- PCRPCR (( RealReal -- timetime reversereverse transcriptasetranscriptase polymerasepolymerase chainchain reactionreaction ) 정량Quantitative

니트로푸란토인에 의해 발현 유도된 상기 실시예 2의 과발현 및 저발현된 유전자 중 니트로푸란토인에 의해 주로 야기되는 폐독성 관련 기전으로 JAK-STAT, TGF-β, MAPK 관련 유전자 18종을 선별하였다. 이들 유전자들은 유전자등록번호(Genebank) NM_053056(Cyclin D1), 유전자등록번호(Genebank) BC069137[Interleukin 5(colony-stimulating factor, eosinophil)], 유전자등록번호(Genebank) NM_003629[Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)], 유전자등록번호(Genebank) NM_003955(Suppressor of cytokine signaling 3), 유전자등록번호(Genebank) NM_002309(Hypothetical protein MGC20647), 유전자등록번호(Genebank) NM_172220[Colony stimulating factor 3(granulocyte)], 유전자등록번호(Genebank) AA995107[Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor(Pan troglodytes)], 유전자등록번호(Genebank) CR618407(Bone morphogenetic protein 2), 유전자등록번호(Genebank) U68019[SMAD, mothers against DPP homolog 3(Drosophila)], 유전자등록번호(Genebank) BM906235(Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), 유전자등록번호(Genebank) NM_006597(Heat shock 70kDa protein 8), 유전자등록번호(Genebank) NM_004465(Fibroblast growth factor 10), 유전자등록번호(Genebank) CR614015(CD14 antigen), 유전자등록번호(Genebank) BC021552(Calcium channel, voltage-dependent, gamma subunit 6), 유전자등록번호(Genebank) AK098095(V-raf murine sarcoma viral oncogene homolog B1), 유전자등록번호(Genebank) BC008678(Interleukin 1, beta), 유전자등록번호(Genebank) NM_000575(Interleukin 1, alpha), 유전자등록번호(Genebank) NM_000944[Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)]이다. Among the overexpressed and underexpressed genes of Example 2 induced by nitrofurantoin, 18 kinds of JAK-STAT, TGF-β, and MAPK related genes were selected as pulmonary toxicity-related mechanisms mainly caused by nitrofurantoin. These genes are genebank NM_053056 (Cyclin D1), genebank BC069137 [Interleukin 5 (colony-stimulating factor, eosinophil)], genebank NM_003629 [Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma)], Genebank NM_003955 (Suppressor of cytokine signaling 3), Genebank NM_002309 (Hypothetical protein MGC20647), Genebank NM_172220 [Colony stimulating factor 3 ( granulocyte), Genebank AA995107 [Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor (Pan troglodytes)], Genebank CR618407 (Bone morphogenetic protein 2), Gene accession number ( Genebank) U68019 [SMAD, mothers against DPP homolog 3 (Drosophila)], Genebank BM906235 (Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein), Genebank NM_006597 (Heat shock 70kDa prot ein 8), Genebank NM_004465 (Fibroblast growth factor 10), Genebank (Genebank) CR614015 (CD14 antigen), Genebank (Genebank) BC021552 (Calcium channel, voltage-dependent, gamma subunit 6), Genebank No. AK098095 (V-raf murine sarcoma viral oncogene homolog B1), Genebank No. (Genebank) BC008678 (Interleukin 1, beta), Genebank No. NM_000575 (Interleukin 1, alpha), Gene Registration No. (Genebank) NM_000944 [Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha)].

상기 유전자들의 발현변화 정도를 조사 및 정량하기 위해 My IQ 실시간 PCR(My IQ Real-time PCR)(Bio-rad, USA)을 이용하여 정량적인 실시간 RT-PCR을 실시하였다. 구체적으로, 올리고 dT 프라이머와 Superscript kit(Omniscipt™ kit, Qiagen, Co., USA)를 이용하여 역전사반응을 수행하여 cDNA를 합성하였다. 상기 cDNA 0.2 ㎕와 물 3.8 ㎕, 센스 프라이머 0.5 ㎕, 안티센스 프라이머 0.5 ㎕, 사이버그린 I 염색 수퍼믹스(Bio-rad, USA) 5 ㎕ 를 혼합하여, PCR 튜브에 담아 단계 1: 95℃, 3분; 단계 2(45회 반복): 단계 2-1: 95℃, 10초; 단계 2-2: 55 내지 65℃ 45초; 단계 3: 95℃, 1분; 단계 4: 55℃, 1분; 단계 5(반복 80회): 55℃, 10초로 프로그램을 설계한 My IQ 실시간 PCR 기계에서 반응을 수행하였다. PCR 산물을 정량하기 위하여 사이버그린(SYBR Green) I 염색(Bio-rad, USA)으로 염색하였다. 사이버그린 I 염색은 이중나선 DNA에 결합하는 염색법으로서, PCR 과정 동안 이중나선 DNA가 생성될수록 형광강도(fluroscense intensity)가 증가하게 된다. 먼저 PCR에 이용한 표적 유전자와 내재성(endogenous) 대조군(GAPDH)에 대한 프라이머를 사이버그린 마스터 믹스(Master mix)에 첨가하여 PCR을 실시한 후, 적절한 농도를 선택하는 프라이머 적합화(primer optimization) 과정을 수행하였다. 합성된 cDNA 시료와 각각의 프라이머(표 4 참조)를 혼합하고, 사이버그린 마스터 믹스를 첨가한 후 PCR를 수행하였고, 정량 소프트웨어(software)를 사용하여 분석하였다(표 5 참조).Quantitative real-time RT-PCR was performed using My IQ Real-time PCR (Bio-rad, USA) to investigate and quantify the expression changes of the genes. Specifically, cDNA was synthesized by performing reverse transcription using an oligo dT primer and a Superscript kit (Omniscipt ™ kit, Qiagen, Co., USA). 0.2 μl of the cDNA, 3.8 μl of water, 0.5 μl of sense primer, 0.5 μl of antisense primer, 5 μl of Cyberrin I stained supermix (Bio-rad, USA), mixed in a PCR tube, Step 1: 95 ° C., 3 minutes ; Step 2 (repeat 45): Step 2-1: 95 ° C., 10 seconds; Step 2-2: 55 to 65 ° C. 45 seconds; Step 3: 95 ° C., 1 minute; Step 4: 55 ° C., 1 minute; Step 5 (80 repetitions): The reaction was performed on a My IQ real-time PCR machine designed program at 55 ° C., 10 seconds. To quantify the PCR products were stained with SYBR Green I stain (Bio-rad, USA). Cyberrin I staining is a staining method that binds to double-stranded DNA. As the double-stranded DNA is generated during PCR, the fluorescence intensity increases. First, primers for the target gene and endogenous control (GAPDH) used for PCR were added to the Cyberrin master mix, followed by PCR, followed by a primer optimization process to select an appropriate concentration. Was performed. Synthesized cDNA samples and each primer (see Table 4) were mixed, PCR was performed after the addition of the Cyberrin master mix and analyzed using quantification software (see Table 5).

프라이머primer 서열 order 등록번호Registration Number 유전자명Gene name PCRPCR 프라이머primer 서열 order (5'->3')(5 '-> 3') NM_053056NM_053056 Cyclin D1Cyclin D1 센스 (서열번호 1)Sense (SEQ ID NO: 1) ACGAAGGTCTGCGCGTGTTACGAAGGTCTGCGCGTGTT 안티센스 (서열번호 2)Antisense (SEQ ID NO: 2) CCGCTGGCCATGAACTACCTCCGCTGGCCATGAACTACCT BC069137BC069137 Interleukin 5(colony-stimulating factor, eosinophil)Interleukin 5 (colony-stimulating factor, eosinophil) 센스 (서열번호 3)Sense (SEQ ID NO: 3) GGCACTGCTTTCTACTCATCGAGGCACTGCTTTCTACTCATCGA 안티센스 (서열번호 4)Antisense (SEQ ID NO: 4) AGTTGGTGATTTTTATGTACAGGAACAAGTTGGTGATTTTTATGTACAGGAACA NM_003629NM_003629 Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) 센스 (서열번호 5)Sense (SEQ ID NO: 5) AGGAATCTTCTGCTCCATGTGGCT AGGAATCTTCTGCTCCATGTGGCT 안티센스 (서열번호 6)Antisense (SEQ ID NO: 6) AAACAGCTGACTCACTCTGAGCCTAAACAGCTGACTCACTCTGAGCCT NM_003955NM_003955 Suppressor of cytokine signaling 3Suppressor of cytokine signaling 3 센스 (서열번호 7)Sense (SEQ ID NO: 7) TTCCAAAGTCTACCTGGTGCCTGATTCCAAAGTCTACCTGGTGCCTGA 안티센스 (서열번호 8)Antisense (SEQ ID NO: 8) TGAAAGATGTCCCGTCTCCTCCAT TGAAAGATGTCCCGTCTCCTCCAT NM_002309NM_002309 Hypothetical protein MGC20647Hypothetical protein MGC20647 센스 (서열번호 9)Sense (SEQ ID NO: 9) TATCACCATCTGTGCCTTTGCTGC TATCACCATCTGTGCCTTTGCTGC 안티센스 (서열번호 10)Antisense (SEQ ID NO: 10) TCTGCCAGATTGTTCCTATGCCCA TCTGCCAGATTGTTCCTATGCCCA NM_172220NM_172220 Colony stimulating factor 3 (granulocyte)Colony stimulating factor 3 (granulocyte) 센스 (서열번호 11)Sense (SEQ ID NO: 11) GGACATGGTTTGACTCCCGAACATGGACATGGTTTGACTCCCGAACAT 안티센스 (서열번호 12)Antisense (SEQ ID NO: 12) ACACACAGGCCTGACATGATTCCT ACACACAGGCCTGACATGATTCCT AA995107AA995107 interleukin 7 receptor [Pan troglodytes]interleukin 7 receptor [Pan troglodytes] 센스 (서열번호 13)Sense (SEQ ID NO: 13) TTGCTCAGGGTCAGCCCATTCTTA TTGCTCAGGGTCAGCCCATTCTTA 안티센스 (서열번호 14)Antisense (SEQ ID NO: 14) TGTCGCTCACGGTAAGTTCAGTCT TGTCGCTCACGGTAAGTTCAGTCT CR618407CR618407 Bone morphogenetic protein 2Bone morphogenetic protein 2 센스 (서열번호 15)Sense (SEQ ID NO: 15) TCACCTCAGCAGAGCTTCAGGTTT TCACCTCAGCAGAGCTTCAGGTTT 안티센스 (서열번호 16)Antisense (SEQ ID NO: 16) ACTTTCCCACCTGCTTGCATTCTG ACTTTCCCACCTGCTTGCATTCTG U68019U68019 SMAD, mothers against DPP homolog 3(Drosophila)SMAD, mothers against DPP homolog 3 (Drosophila) 센스 (서열번호 17)Sense (SEQ ID NO: 17) AGTGCTGGTGACTGGATAGCAGTT AGTGCTGGTGACTGGATAGCAGTT 안티센스 (서열번호 18)Antisense (SEQ ID NO: 18) GCACAAGCTGCAAGGTGAAGATGT GCACAAGCTGCAAGGTGAAGATGT BM906235BM906235 Inhibitor of DNA binding 3, dominant negative helix-loop-helix proteinInhibitor of DNA binding 3, dominant negative helix-loop-helix protein 센스 (서열번호 19)Sense (SEQ ID NO: 19) TCAGCTTAGCCAGGTGGAAATCTCAGCTTAGCCAGGTGGAAATC 안티센스 (서열번호 20)Antisense (SEQ ID NO: 20) TGGCTCGGCCAGGACTACTGGCTCGGCCAGGACTAC NM_006597NM_006597 Heat shock 70kDa protein 8Heat shock 70kDa protein 8 센스 (서열번호 21)Sense (SEQ ID NO: 21) TCCAAGGGACCTGCAGTTGGTATT TCCAAGGGACCTGCAGTTGGTATT 안티센스 (서열번호 22)Antisense (SEQ ID NO: 22) ACTTGATTCTTTGCGGCATCACCG ACTTGATTCTTTGCGGCATCACCG NM_004465NM_004465 Fibroblast growth factor 10Fibroblast growth factor 10 센스 (서열번호 23)Sense (SEQ ID NO: 23) CATTGTGCCTCAGCCTTTC CATTGTGCCTCAGCCTTTC 안티센스 (서열번호 24)Antisense (SEQ ID NO: 24) TCCATTTTCCTCTATCCTCTCCTCCATTTTCCTCTATCCTCTCC CR614015CR614015 CD14 antigenCD14 antigen 센스 (서열번호 25)Sense (SEQ ID NO: 25) GAATGGACTCAAACTGCCTTGGCT GAATGGACTCAAACTGCCTTGGCT 안티센스 (서열번호 26)Antisense (SEQ ID NO: 26) GGGCAAAGGGTTGAATTGGTCGAA GGGCAAAGGGTTGAATTGGTCGAA BC021552BC021552 Calcium channel, voltage-dependent, gamma subunit 6Calcium channel, voltage-dependent, gamma subunit 6 센스 (서열번호 27)Sense (SEQ ID NO: 27) TCTGTGGCTGTATGTGGGTTGCTT TCTGTGGCTGTATGTGGGTTGCTT 안티센스 (서열번호 28)Antisense (SEQ ID NO: 28) AGTGAATCGATCCAGACCTGGGAA AGTGAATCGATCCAGACCTGGGAA AK098095AK098095 V-raf murine sarcoma viral oncogene homolog B1V-raf murine sarcoma viral oncogene homolog B1 센스 (서열번호 29)Sense (SEQ ID NO: 29) TTTCCAGGGTTTCCGCTGTCAAAC TTTCCAGGGTTTCCGCTGTCAAAC 안티센스 (서열번호 30)Antisense (SEQ ID NO: 30) TCCTGTGGTATTGGGTGGTGTTCATCCTGTGGTATTGGGTGGTGTTCA BC008678BC008678 Interleukin 1, betaInterleukin 1, beta 센스 (서열번호 31)Sense (SEQ ID NO: 31) TCCCCAGCCCTTTTGTTGATCCCCAGCCCTTTTGTTGA 안티센스 (서열번호 32)Antisense (SEQ ID NO: 32) TTAGAACCAAATGTGGCCGTGTTAGAACCAAATGTGGCCGTG NM_000575NM_000575 Interleukin 1, alphaInterleukin 1, alpha 센스 (서열번호 33)Sense (SEQ ID NO: 33) AGGCATCCTCCACAATAGCAGACAAGGCATCCTCCACAATAGCAGACA 안티센스 (서열번호 34)Antisense (SEQ ID NO: 34) TCGGACCAATTACTGGCTCAAGGTTCGGACCAATTACTGGCTCAAGGT NM_000944NM_000944 Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) 센스 (서열번호 35)Sense (SEQ ID NO: 35) TTGATGCGCCAGTCACTGTTTGTGTTGATGCGCCAGTCACTGTTTGTG 안티센스 (서열번호 36)Antisense (SEQ ID NO: 36) AAGAAGAGGTAGCGAGTGTTGGCA AAGAAGAGGTAGCGAGTGTTGGCA

등록번호Registration Number 유전자명Gene name 실시간 real time PCRPCR (상대적 배율)(Relative magnification) cDNAcDNA 마이크로어레이Microarray (( Cy3Cy3 /Of Cy5Cy5 비율) ratio) NM_006597NM_006597 Heat shock 70kDa protein 8Heat shock 70kDa protein 8 1.763 1.763 2.205 2.205 NM_053056NM_053056 Cyclin D1Cyclin D1 1.765 1.765 2.114 2.114 BC069137BC069137 Interleukin 5(colony-stimulating factor, eosinophil)Interleukin 5 (colony-stimulating factor, eosinophil) 1.862 1.862 2.034 2.034 NM_004465NM_004465 Fibroblast growth factor 10Fibroblast growth factor 10 1.578 1.578 2.018 2.018 U68019U68019 SMAD, mothers against DPP homolog 3(Drosophila)SMAD, mothers against DPP homolog 3 (Drosophila) 0.468 0.468 0.500 0.500 CR614015CR614015 CD14 antigenCD14 antigen 0.509 0.509 0.500 0.500 CR618407CR618407 Bone morphogenetic protein 2Bone morphogenetic protein 2 0.375 0.375 0.492 0.492 BM906235BM906235 Inhibitor of DNA binding 3, dominant negative helix-loop-helix proteinInhibitor of DNA binding 3, dominant negative helix-loop-helix protein 0.475 0.475 0.485 0.485 NM_003629NM_003629 Phosphoinositide-3-kinase, regulatory subunit 3(p55, gamma)Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) 0.552 0.552 0.473 0.473 NM_003955NM_003955 Suppressor of cytokine signaling 3Suppressor of cytokine signaling 3 0.601 0.601 0.472 0.472 BC021552BC021552 Calcium channel, voltage-dependent, gamma subunit 6Calcium channel, voltage-dependent, gamma subunit 6 0.533 0.533 0.452 0.452 AK098095AK098095 V-raf murine sarcoma viral oncogene homolog B1V-raf murine sarcoma viral oncogene homolog B1 0.345 0.345 0.422 0.422 NM_002309NM_002309 Hypothetical protein MGC20647Hypothetical protein MGC20647 0.522 0.522 0.415 0.415 BC008678BC008678 Interleukin 1, betaInterleukin 1, beta 0.546 0.546 0.393 0.393 NM_000575NM_000575 Interleukin 1, alphaInterleukin 1, alpha 0.265 0.265 0.362 0.362 NM_172220NM_172220 Colony stimulating factor 3(granulocyte)Colony stimulating factor 3 (granulocyte) 0.291 0.291 0.321 0.321 AA995107AA995107 interleukin 7 receptor [Pan troglodytes]interleukin 7 receptor [Pan troglodytes] 0.347 0.347 0.292 0.292 NM_000944NM_000944 Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) 0.402 0.402 0.229 0.229

그 결과, 4종의 과발현 유전자들과 14종의 저발현 유전자들의 유전자 발현 양상은 폐독성 유발 약물들에 의한 유전자 발현을 조사한 올리고 마이크로어레이 결과와 매우 유사하게 나타남을 확인하였다.As a result, it was confirmed that the gene expression patterns of four overexpression genes and 14 low expression genes were very similar to those of oligo microarrays which examined gene expression by pulmonary toxicity-inducing drugs.

도 1은 니트로푸란토인의 인간 기관지 상피 세포주에서의 세포 독성을 조사한 그래프이고,1 is a graph illustrating the cytotoxicity of nitrofurantoin in human bronchial epithelial cell line,

도 2는 마이크로어레이 칩을 이용한 니트로푸란토인을 처리한 인간 기관지 상피 세포주의 유전자 발현 양상을 분석한 결과를 나타낸 그림이다.Figure 2 is a diagram showing the results of analyzing the gene expression pattern of human bronchial epithelial cell line treated with nitrofurantoin using a microarray chip.

<110> Korea Institute of Science and Technology <120> Marker genes based on nitrofurantoin treatment for screening of drug inducing pulmonary toxicity and screening method using thereof <130> 7p-12-28 <160> 36 <170> KopatentIn 1.71 <210> 1 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Cyclin D1 sense primer <400> 1 acgaaggtct gcgcgtgtt 19 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyclin D1 antisense primer <400> 2 ccgctggcca tgaactacct 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 5 sense primer <400> 3 ggcactgctt tctactcatc ga 22 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 5 antisense primer <400> 4 agttggtgat ttttatgtac aggaaca 27 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Phosphoinositide-3-kinase, regulatory subunit 3 sense primer <400> 5 aggaatcttc tgctccatgt ggct 24 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Phosphoinositide-3-kinase, regulatory subunit 3 antisense primer <400> 6 aaacagctga ctcactctga gcct 24 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Suppressor of cytokine signaling 3 sense primer <400> 7 ttccaaagtc tacctggtgc ctga 24 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Suppressor of cytokine signaling 3 antisense primer <400> 8 tgaaagatgt cccgtctcct ccat 24 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Hypothetical protein MGC20647 sense primer <400> 9 tatcaccatc tgtgcctttg ctgc 24 <210> 10 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Hypothetical protein MGC20647 antisense primer <400> 10 tctgccagat tgttcctatg ccca 24 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Colony stimulating factor 3 sense primer <400> 11 ggacatggtt tgactcccga acat 24 <210> 12 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Colony stimulating factor 3 antisense primer <400> 12 acacacaggc ctgacatgat tcct 24 <210> 13 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> interleukin 7 receptor sense primer <400> 13 ttgctcaggg tcagcccatt ctta 24 <210> 14 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> interleukin 7 receptor antisense primer <400> 14 tgtcgctcac ggtaagttca gtct 24 <210> 15 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Bone morphogenetic protein 2 sense primer <400> 15 tcacctcagc agagcttcag gttt 24 <210> 16 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Bone morphogenetic protein 2 antisense primer <400> 16 actttcccac ctgcttgcat tctg 24 <210> 17 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SMAD, mothers against DPP homolog 3 sense primer <400> 17 agtgctggtg actggatagc agtt 24 <210> 18 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SMAD, mothers against DPP homolog 3 antisense primer <400> 18 gcacaagctg caaggtgaag atgt 24 <210> 19 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein sense primer <400> 19 tcagcttagc caggtggaaa tc 22 <210> 20 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein antisense primer <400> 20 tggctcggcc aggactac 18 <210> 21 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Heat shock 70kDa protein 8 sense primer <400> 21 tccaagggac ctgcagttgg tatt 24 <210> 22 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Heat shock 70kDa protein 8 antisense primer <400> 22 acttgattct ttgcggcatc accg 24 <210> 23 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Fibroblast growth factor 10 sense primer <400> 23 cattgtgcct cagcctttc 19 <210> 24 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Fibroblast growth factor 10 antisense primer <400> 24 tccattttcc tctatcctct cc 22 <210> 25 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CD14 antigen sense primer <400> 25 gaatggactc aaactgcctt ggct 24 <210> 26 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CD14 antigen antisense primer <400> 26 gggcaaaggg ttgaattggt cgaa 24 <210> 27 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Calcium channel, voltage-dependent, gamma subunit 6 sense primer <400> 27 tctgtggctg tatgtgggtt gctt 24 <210> 28 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Calcium channel, voltage-dependent, gamma subunit 6 antisense primer <400> 28 agtgaatcga tccagacctg ggaa 24 <210> 29 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> V-raf murine sarcoma viral oncogene homolog B1 sense primer <400> 29 tttccagggt ttccgctgtc aaac 24 <210> 30 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> V-raf murine sarcoma viral oncogene homolog B1 antisense primer <400> 30 tcctgtggta ttgggtggtg ttca 24 <210> 31 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, beta sense primer <400> 31 tccccagccc ttttgttga 19 <210> 32 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, beta antisense primer <400> 32 ttagaaccaa atgtggccgt g 21 <210> 33 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, alpha sense primer <400> 33 aggcatcctc cacaatagca gaca 24 <210> 34 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, alpha antisense primer <400> 34 tcggaccaat tactggctca aggt 24 <210> 35 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform sense primer <400> 35 ttgatgcgcc agtcactgtt tgtg 24 <210> 36 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Protein phosphatase 3(formerly 2B), catalytic subunit, alpha isoform antisense primer <400> 36 aagaagaggt agcgagtgtt ggca 24 <110> Korea Institute of Science and Technology <120> Marker genes based on nitrofurantoin treatment for screening of          drug inducing pulmonary toxicity and screening method using          the <130> 7p-12-28 <160> 36 <170> KopatentIn 1.71 <210> 1 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Cyclin D1 sense primer <400> 1 acgaaggtct gcgcgtgtt 19 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyclin D1 antisense primer <400> 2 ccgctggcca tgaactacct 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 5 sense primer <400> 3 ggcactgctt tctactcatc ga 22 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 5 antisense primer <400> 4 agttggtgat ttttatgtac aggaaca 27 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Phosphoinositide-3-kinase, regulatory subunit 3 sense primer <400> 5 aggaatcttc tgctccatgt ggct 24 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Phosphoinositide-3-kinase, regulatory subunit 3 antisense primer <400> 6 aaacagctga ctcactctga gcct 24 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Suppressor of cytokine signaling 3 sense primer <400> 7 ttccaaagtc tacctggtgc ctga 24 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Suppressor of cytokine signaling 3 antisense primer <400> 8 tgaaagatgt cccgtctcct ccat 24 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Hypothetical protein MGC20647 sense primer <400> 9 tatcaccatc tgtgcctttg ctgc 24 <210> 10 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Hypothetical protein MGC20647 antisense primer <400> 10 tctgccagat tgttcctatg ccca 24 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Colony stimulating factor 3 sense primer <400> 11 ggacatggtt tgactcccga acat 24 <210> 12 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Colony stimulating factor 3 antisense primer <400> 12 acacacaggc ctgacatgat tcct 24 <210> 13 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> interleukin 7 receptor sense primer <400> 13 ttgctcaggg tcagcccatt ctta 24 <210> 14 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> interleukin 7 receptor antisense primer <400> 14 tgtcgctcac ggtaagttca gtct 24 <210> 15 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Bone morphogenetic protein 2 sense primer <400> 15 tcacctcagc agagcttcag gttt 24 <210> 16 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Bone morphogenetic protein 2 antisense primer <400> 16 actttcccac ctgcttgcat tctg 24 <210> 17 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SMAD, mothers against DPP homolog 3 sense primer <400> 17 agtgctggtg actggatagc agtt 24 <210> 18 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SMAD, mothers against DPP homolog 3 antisense primer <400> 18 gcacaagctg caaggtgaag atgt 24 <210> 19 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Inhibitor of DNA binding 3, dominant negative helix-loop-helix          protein sense primer <400> 19 tcagcttagc caggtggaaa tc 22 <210> 20 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Inhibitor of DNA binding 3, dominant negative helix-loop-helix          protein antisense primer <400> 20 tggctcggcc aggactac 18 <210> 21 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Heat shock 70kDa protein 8 sense primer <400> 21 tccaagggac ctgcagttgg tatt 24 <210> 22 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Heat shock 70kDa protein 8 antisense primer <400> 22 acttgattct ttgcggcatc accg 24 <210> 23 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Fibroblast growth factor 10 sense primer <400> 23 cattgtgcct cagcctttc 19 <210> 24 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Fibroblast growth factor 10 antisense primer <400> 24 tccattttcc tctatcctct cc 22 <210> 25 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CD14 antigen sense primer <400> 25 gaatggactc aaactgcctt ggct 24 <210> 26 <211> 24 <212> DNA <213> Artificial Sequence <220> CD223 antigen antisense primer <400> 26 gggcaaaggg ttgaattggt cgaa 24 <210> 27 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Calcium channel, voltage-dependent, gamma subunit 6 sense primer <400> 27 tctgtggctg tatgtgggtt gctt 24 <210> 28 <211> 24 <212> DNA <213> Artificial Sequence <220> 223 Calcium channel, voltage-dependent, gamma subunit 6 antisense          primer <400> 28 agtgaatcga tccagacctg ggaa 24 <210> 29 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> V-raf murine sarcoma viral oncogene homolog B1 sense primer <400> 29 tttccagggt ttccgctgtc aaac 24 <210> 30 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> V-raf murine sarcoma viral oncogene homolog B1 antisense primer <400> 30 tcctgtggta ttgggtggtg ttca 24 <210> 31 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, beta sense primer <400> 31 tccccagccc ttttgttga 19 <210> 32 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, Beta Antisense Primer <400> 32 ttagaaccaa atgtggccgt g 21 <210> 33 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, alpha sense primer <400> 33 aggcatcctc cacaatagca gaca 24 <210> 34 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Interleukin 1, alpha antisense primer <400> 34 tcggaccaat tactggctca aggt 24 <210> 35 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha          isoform sense primer <400> 35 ttgatgcgcc agtcactgtt tgtg 24 <210> 36 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha          isoform antisense primer <400> 36 aagaagaggt agcgagtgtt ggca 24  

Claims (16)

하기의 군으로부터 선택되는 어느 하나 이상의 유전자의 핵산서열의 전부 또는 18 내지 30개의 핵산 서열로 구성되는 상기 유전자의 단편인 올리고뉴클레오티드 또는 그의 상보가닥 분자가 집적된 니트로푸란토인 처리에 대한 JAK-STAT 신호경로(signaling pathway) 관련 기능을 갖는 유전자 발현 여부 확인용 DNA 마이크로어레이 칩:JAK-STAT signaling for treatment of oligonucleotides or fragments of the above genes consisting of 18-30 nucleic acid sequences or all of the nucleic acid sequences of any one or more genes selected from the following: DNA microarray chip for gene expression with signaling pathway related functions: 유전자등록번호(Genebank) NM_053056(Cyclin D1), 유전자등록번호(Genebank) BC069137[Interleukin 5 (colony-stimulating factor, eosinophil), 유전자등록번호(Genebank) NM_003629[Phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma)], 유전자등록번호(Genebank) NM_003955(Suppressor of cytokine signaling 3), 유전자등록번호(Genebank) NM_002309(Hypothetical protein MGC20647), 유전자등록번호(Genebank) NM_172220[Colony stimulating factor 3 (granulocyte)], 유전자등록번호(Genebank) AA995107[Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor (Pan troglodytes)].Genebank NM_053056 (Cyclin D1), Genebank BC069137 [Interleukin 5 (colony-stimulating factor, eosinophil), Genebank NM_003629 [Phosphoinositide-3-kinase, regulatory subunit 3 (p55) , gamma)], Genebank NM_003955 (Suppressor of cytokine signaling 3), Genebank NM_002309 (Hypothetical protein MGC20647), Genebank NM_172220 [Colony stimulating factor 3 (granulocyte)], Genebank AA995107 (Transcribed locus, moderately similar to XP_517807.1 PREDICTED: interleukin 7 receptor (Pan troglodytes)]. 삭제delete 삭제delete 삭제delete 삭제delete 1) 인간 기관지 세포 또는 폐 세포에 피검화합물을 처리하는 단계;1) treating the test compound with human bronchial cells or lung cells; 2) 단계 1)의 피검화합물을 처리한 실험군 세포와 피검화합물을 처리하지 않은 대조군 세포에서 RNA를 분리하는 단계;2) separating RNA from the test cell treated with the test compound of step 1) and the control cell not treated with the test compound; 3) 단계 2)의 실험군 및 대조군의 RNA를 cDNA로 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;3) labeling the experimental group and the control group with different fluorescent materials while synthesizing the RNA of the experimental group and the control group of step 2) with cDNA; 4) 단계 3)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 DNA 마이크로어레이칩과 혼성화시키는 단계;4) hybridizing cDNA labeled with different fluorescent materials of step 3) with the DNA microarray chip of claim 1; 5) 반응한 DNA 마이크로어레이칩을 분석하는 단계; 및5) analyzing the reacted DNA microarray chip; And 6) 분석한 데이터에서 제 1항의 DNA 마이크로어레이칩에 집적된 유전자의 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 니트로푸란토인 처리에 대한 JAK-STAT 신호경로(signaling pathway) 관련 기능을 갖는 유전자 발현 여부 확인 방법.6) The JAK-STAT signaling pathway related function for nitrofurantoin treatment comprising the step of confirming the expression level of the gene integrated in the DNA microarray chip of claim 1 in the analyzed data compared with the control group. How to check whether the gene has. 제 6항에 있어서, 단계 1)의 인간 기관지 세포는 인간 기관지 상피 세포인 BEAS-2B 세포인 것을 특징으로 하는 방법.The method of claim 6, wherein the human bronchial cells of step 1) are BEAS-2B cells, which are human bronchial epithelial cells. 제 6항에 있어서, 단계 3)의 형광물질은 Cy3, Cy5, 폴리 L-라이신-플루오레세인 이소티오시아네이트(poly L-lysine-fluorescein isothiocyanate, FITC), 로다민-B-이소티오시아네이트(rhodamine-B-isothiocyanate, RITC), 로다민(rhodamine)으로 이루어진 군으로부터 선택된 어느 하나인 것을 특징으로 하는 방법.The method of claim 6, wherein the fluorescent material of step 3) is Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate (rhodamine-B-isothiocyanate, RITC), rhodamine (rhodamine), characterized in that any one selected from the group consisting of. 1) 인간 기관지 세포 또는 폐 세포에 피검화합물을 처리하는 단계;1) treating the test compound with human bronchial cells or lung cells; 2) 단계 1)의 피검화합물을 처리한 실험군 세포와 처리하지 않은 대조군 세포에서 RNA를 분리하는 단계;2) separating RNA from experimental cells treated with the test compound of step 1) and untreated control cells; 3) 단계 2)의 RNA를, 제 1항의 마이크로어레이칩에 집적된 유전자에 상보적이고 상기 유전자를 증폭할 수 있는 18머 내지 30머 길이의 프라이머쌍을 사용하여 실시간 RT-PCR(Real-time reverse transcript polymerase chain reaction)을 수행하는 단계; 및3) Real-time reverse real-time reverse RT-PCR using RNA pairs of 18 to 30mer length complementary to the gene integrated in the microarray chip of claim 1 and amplifying the gene of step 2) performing a transcript polymerase chain reaction; And 4) 단계 3)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 니트로푸란토인 처리에 대한 JAK-STAT 신호경로(signaling pathway) 관련 기능을 갖는 유전자 발현 여부 확인 방법.4) Checking the expression of the gene having a JAK-STAT signaling pathway related function for nitrofurantoin treatment, comprising the step of confirming the expression level by comparing the gene product of step 3) with the control. 제 9항에 있어서, 단계 1)의 인간 기관지 세포는 인간 기관지 상피 세포인 BEAS-2B 세포인 것을 특징으로 하는 방법.10. The method of claim 9, wherein the human bronchial cells of step 1) are BEAS-2B cells, which are human bronchial epithelial cells. 제 1항의 DNA 마이크로어레이 칩을 포함하는, 니트로푸란토인 처리에 대한 JAK-STAT 신호경로(signaling pathway) 관련 기능을 갖는 유전자 발현 여부 확인용 키트.Claim 1 kit comprising a DNA microarray chip, a gene expression check kit having a JAK-STAT signaling pathway related functions for nitrofurantoin treatment. 제 11항에 있어서, BEAS-2B 세포, 인간 기관지 세포 또는 폐 세포를 추가적으로 포함하는 것을 특징으로 하는 키트.The kit of claim 11, further comprising BEAS-2B cells, human bronchial cells, or lung cells. 제 11항에 있어서, 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin- like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 키트.The method of claim 11, further comprising any one selected from the group of fluorescent substances consisting of streptadin-like phosphatease conjugates, chemilurorensce and chemiluminescent. Kit characterized in that. 제 11항에 있어서, 혼성화에 사용되는 완충용액, RNA로부터 cDNA를 합성하기 위한 역전사효소, cNTPs 및 rNTP(사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 키트.The method according to claim 11, which is selected from the group of reaction reagents consisting of a buffer solution used for hybridization, reverse transcriptase for synthesizing cDNA from RNA, cNTPs and rNTP (premixed or separated feed), a marker reagent, and a wash buffer. Kit comprising any one further. 제 1항의 DNA 마이크로어레이 칩에 집적된 유전자에 상보적이고, 상기 유전자를 증폭할 수 있는 18머 내지 30머 길이의 프라이머쌍을 포함하는, 니트로푸란토인 처리에 대한 JAK-STAT 신호경로(signaling pathway) 관련 기능을 갖는 유전자 발현 여부 확인용 키트.The JAK-STAT signaling pathway for nitrofurantoin treatment comprising a primer pair of 18 to 30mer length that is complementary to the gene integrated in the DNA microarray chip of claim 1 and capable of amplifying the gene. Kit for confirming the expression of genes with related functions. 삭제delete
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