KR100564227B1 - Cosmetic material composition for skin whitening - Google Patents

Cosmetic material composition for skin whitening Download PDF

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KR100564227B1
KR100564227B1 KR1019990040578A KR19990040578A KR100564227B1 KR 100564227 B1 KR100564227 B1 KR 100564227B1 KR 1019990040578 A KR1019990040578 A KR 1019990040578A KR 19990040578 A KR19990040578 A KR 19990040578A KR 100564227 B1 KR100564227 B1 KR 100564227B1
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extract
designated
extracts
wasong
new song
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KR20010028372A (en
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김호정
김청택
진무현
김진준
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주식회사 엘지생활건강
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

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  • Dermatology (AREA)
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  • Cosmetics (AREA)

Abstract

본 발명은 매우 강력한 멜라닌 생성 억제 효과와 미백 효과가 있는 화장료 조성물을 제공하기 위하여, 지정, 와송 및 신곡 추출물로 이루어진 군으로부터 선택되어진 1 종 이상의 추출물을 유효 성분으로 함유하는 것을 특징으로하는 피부 미백용 화장료 조성물을 제공한다.The present invention is to provide a cosmetic composition having a very strong melanin production inhibitory effect and whitening effect, for skin whitening, characterized in that it contains at least one extract selected from the group consisting of designated, Wasong and Singok extract as an active ingredient It provides a cosmetic composition.

지정, 와송, 신곡, 멜라닌 생성 억제능, 피부 과색소 침착증, 티로시나제, 하이드로퀴논, 티올계 화합물, 미백제, 멜라노마 세포Designation, wasong, new song, melanin production inhibitory ability, hyperpigmentation, tyrosinase, hydroquinone, thiol compound, whitening agent, melanoma cell

Description

피부 미백용 화장료 조성물{COSMETIC MATERIAL COMPOSITION FOR SKIN WHITENING}Cosmetic composition for skin whitening {COSMETIC MATERIAL COMPOSITION FOR SKIN WHITENING}

[산업상 이용분야][Industrial use]

본 발명은 피부 미백용 화장료 조성물에 관한 것으로서, 보다 상세하게는 멜라닌 생성 억제 효과가 우수한 지정, 와송 및 신곡 추출물로 이루어진 군으로부터 선택된 1 종 이상의 추출물을 유효 성분으로 함유하는 피부 미백용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for skin whitening, and more particularly to a cosmetic composition for skin whitening containing, as an active ingredient, one or more extracts selected from the group consisting of designated, wasong and singok extracts having an excellent melanin production inhibitory effect. will be.

[종래 기술][Prior art]

희고 고운 피부를 갖고자 하는 것은 모든 사람의 한결같은 소망이다. 사람의 피부색은 피부내 멜라닌(melanin)의 농도와 분포에 따라 유전적으로 결정되나, 태양 자외선이나 피로, 스트레스 등의 환경적 또는 생리적 조건에 의해서도 영향을 받는다. 멜라닌은 아미노산의 일종인 티로신(tyrosine)에 티로시나제(tyrosinase)라는 효소가 작용하여 도파(DOPA), 도파퀴논(dopaquinone)으로 바뀐 후 비효소적인 산화 반응을 거쳐 만들어진다. 그러나 멜라닌이 만들어지는 경로는 알려져 있으나, 티로시나제가 작용하는 이전 단계인 멜라닌 합성을 유도하는 메카니즘 (mechanism)이 무엇인지에 대해서는 아직도 자세히 밝혀지지 않고 있다. It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also influenced by environmental or physiological conditions such as solar ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosine) enzymes called tyrosinase (tyrosinase) acts to convert to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still not clear what mechanism is responsible for inducing melanin synthesis, the previous step in which tyrosinase works.

한편 멜라닌 색소의 합성을 저해함으로써 피부 톤을 밝게하여 피부 미백을 실현할 수 있을 뿐만 아니라 자외선, 호르몬, 유전에 기인한 기미, 자외선, 유전에 기인한 주근깨 등의 피부 과색소 침착증의 개선이 가능하다.On the other hand, by inhibiting the synthesis of melanin pigment, not only the skin tone can be brightened to realize skin whitening, but also the improvement of skin hyperpigmentation, such as blemishes caused by ultraviolet rays, hormones and heredity, ultraviolet rays, and freckles caused by heredity.

이에 종래에는 하이드로퀴논(hydroquinone)이나 아스콜빈산(ascorbic acid), 고지산(kojic acid), 글루타치온(glutathione)같은 티로시나제에 저해 활성을 갖는 물질을 연고나 화장료에 배합하여 피부 미백이나 기미, 주근깨 개선의 목적에 사용하여 왔으나, 하이드로퀴논의 경우 미백 효과는 인정되지만 피부 자극성이 심하여 사용량이 극히 제한된다. 또한 아스콜빈산은 산화되기 쉬워 이를 배합한 화장료는 변색, 변취되는 등의 문제를 야기하며, 글루타치온, 시스테인 등의 티올계 화합물은 특유의 불쾌한 냄새가 나며 경피 흡수에 문제가 있다. 또한 이들의 배당체 및 유도체들은 극성이 높아 화장료의 배합 성분으로 사용하기에는 문제가 있다. 또한 태반 추출물 등은 미백 효과가 불충분하다는 문제가 있다.Therefore, conventionally, a substance having inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione, and the like is added to the ointment or cosmetics to improve skin whitening, blemishes and freckles. Although it has been used for the purpose of hydroquinone, the whitening effect is recognized, but the skin irritation is severe, the amount of use is extremely limited. In addition, ascorbic acid is easily oxidized, and the cosmetics containing the same cause problems such as discoloration and deodorization, and thiol-based compounds such as glutathione and cysteine have a characteristic unpleasant odor and have problems in transdermal absorption. In addition, these glycosides and derivatives have a high polarity, so there is a problem to use them as a component of a cosmetic. In addition, placenta extract has a problem that the whitening effect is insufficient.

따라서 본 발명은 상기 문제점들을 극복할 수 있는 멜라닌 생성 억제 효과와 기미, 주근깨 등의 피부 과색소 침착증의 개선 및 피부 미백 효과가 있는 피부 미백용 화장료 조성물을 제공하는 것을 목적으로한다.Therefore, an object of the present invention is to provide a cosmetic composition for skin whitening, which has an effect of inhibiting melanin production and improvement of skin hyperpigmentation, such as blemishes and freckles, and skin whitening effect which can overcome the above problems.

[과제를 해결하기위한 수단][Means for solving the problem]

상기 목적을 달성하기 위하여, 본 발명은 지정, 와송 및 신곡 추출물로 이루 어진 군으로부터 선택되어진 1 종 이상의 추출물을 유효 성분으로 함유하는 피부 미백용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening containing as an active ingredient one or more extracts selected from the group consisting of designated, zinnia and new song extract.

상기 피부 미백용 화장료 조성물은 지정, 와송 및 신곡 추출물을 건조 중량으로 0.0001 - 10.0 중량% 함유하는 것이 바람직하다.The cosmetic composition for skin whitening preferably contains 0.0001-10.0% by weight of the designated, Wasong and new song extract in dry weight.

또한, 상기 지정, 와송 및 신곡 추출물은 지정, 와송 및 신곡을 물, 탄소수 1 - 4의 저급 알콜, 에틸아세테이트 또는 아세톤으로 1 회 이상 추출하여 추출액을 얻고, 상기 추출액을 감압 증류한 후 동결 건조하거나 분사 건조하여 얻는 것이 바람직하다.In addition, the designated, Whasong and new song extract is obtained by extracting the designated, Whasong and new song with water, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone one or more times to obtain an extract, the extract is distilled under reduced pressure and freeze-dried It is preferable to obtain by spray drying.

이하, 본 발명을 상세하게 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail.

지정은 다년생 초본으로 제비꽃과 식물인 Viola yedoensis Mak.과 Vmandshurica W. Becker, 콩과 식물인 Gueldenstaedtia multiflora와 Gueldenstaedtia pauciflora, 용담과 식물인 Gentiana loureiri를 포함하며(중약대사전) 뿌리를 포함하는 전초가 청열이습, 해독소종 등의 한방 약재로 사용되어 왔다(동의보감). 또한 지정 추출물을 포함하는 가려움증 치료용 냅킨(napkin)에 관한 특허(중국특허 1101956)가 있으나 본 발명과는 무관하다. 와송은 경천과 (Crassulaceae)에 속하며 라틴명은 Herba Orostachydis이고 학명은 Orostachys fimbriatus, Orostachys japonicus로서 전초를 사용하여 토혈, 비출혈, 혈이, 간염, 말라리아, 치질, 습진, 화상을 치료하는데 사용되어 왔다(중약대사전). 기존의 와송 성분 동정 연구에 의하면 β-Amyrin, Friedelin, epi-Friedelinol, Glutinone 등의 화합물이 와송의 성분으로서 보고 되어 있다. 신곡은 행인, 개똥 쑥, 도꼬마리 등의 생약을 밀가루 또는 밀기울과 섞어 발효시켜 만든 누룩으로 라틴명은 Massa Medicata Fermentat이며 소화불량, 산후 어혈에 의한 복통을 치료하는데 사용되어 왔다(중약대사전).Designations include perennial herbaceous plants, including Viola yedoensis Mak. And Vmandshurica W. Becker, legumes Gueldenstaedtia multiflora and Gueldenstaedtia pauciflora, gentiana loureiri, gentiana loureiri (Chinese medicinal dictionary) It has been used as herbal medicines such as moist and detoxification. In addition, there is a patent (Chinese Patent 1101956) relating to a napkin for treating itching, including a designated extract, but it is not related to the present invention. Wasong belongs to the Crassulaceae, Latin is Herba Orostachydis and scientific name Orostachys fimbriatus, Orostachys japonicus has been used to treat hemorrhage, nasal bleeding, hemostasis, hepatitis, malaria, hemorrhoids, eczema, and burns by using outposts. Chinese medicine dictionary). According to the existing research on identification of vortices, compounds such as β-Amyrin, Friedelin, epi-Friedelinol, and Glutinone have been reported as constituents of vortex. Singok is a yeast made by mixing fermented herbs such as almonds, firewood, and oxtail with wheat flour or wheat bran. The Latin name is Massa Medicata Fermentat.

지정, 와송 및 신곡 추출물을 얻기 위하여 생약재로 시판되는 지정, 와송 및 신곡을 구입하여 잘게 분쇄하고 분쇄물 건조 중량에 대하여 5 - 20 부피의 물이나 탄소수 1 - 4 개의 무수 또는 함수 저급 알콜, 에틸아세테이트 또는 아세톤과 혼합한 후, 환류 냉각기가 달린 추출기에서 50 - 100℃로 1 - 5 시간 가열하여 추출한다. 여과포로 여과한 후 잔사를 같은 방법으로 1 회 이상씩 더 추출한다. 추출액을 합하여 감압 농축한 후 동결 건조나 분무 건조하여 건조 추출물을 얻는다.Purchased commercially available medicinal herbs, locusts and new songs, to obtain designated extracts, and extracts and extracts, and finely pulverize. 5 to 20 volumes of water or 1 to 4 anhydrous or hydrous lower alcohols, ethyl acetate Or mixed with acetone and then extracted by heating at 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser. After filtering with a filter cloth, the residue is further extracted one or more times in the same manner. The extracts are combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract.

건조된 지정, 와송 및 신곡 추출물을 쥐의 멜라노마 세포(B-16 melanoma cell)에 처리하였을 때, 매우 강력한 멜라닌 합성 저해 효과를 나타내었다.When the dried extracts, hawsong and singok extracts were treated with rat B-16 melanoma cells, they showed very potent melanin synthesis inhibitory effects.

상기와 같이 얻어진 지정, 와송 및 신곡 추출물을 함유한 피부 외용 연고나 로션 같은 여러 화장료를 제조한다. 지정, 와송 및 신곡 추출물을 함유한 피부 외용 연고나 로션 등의 화장료를 제조하여 사람의 피부에 도포하였을 때 피부 미백 효과가 매우 우수하였다. 또한 어떤 부작용도 발생하지 않아 지정, 와송 및 신곡 추출물이 안전하고 효과가 매우 뛰어난 기미나 주근깨 개선 및 피부 미백제임을 알 수 있다.Various cosmetics such as skin ointments and lotions containing the designated extracts, vortex and new song extract obtained as described above are prepared. The skin whitening effect, such as ointment, lotion, and lotion containing the extracts of the spermatozoon and singok, was prepared and applied to human skin. In addition, no side effects occur, indicating that the designated extracts, Wasong and new song extracts are safe and highly effective for improving blemishes and freckles and skin whitening agents.

피부 외용 연고나 화장료 제조시에 함유되는 지정, 와송 및 신곡 추출물의 함량은 건조 중량으로 0.0001 - 10.0 중량%, 바람직하게는 0.0005 - 10.0 중량%가 적당하다. 0.0001 중량% 이하의 농도에서는 뚜렸한 효과를 기대할 수 없었고, 10 중량% 이상의 농도에서는 함유량의 증가에 따라 뚜렷한 효과의 증가가 나타나지 않았다.The content of designated extracts, vortices, and new song extracts contained in the preparation of external skin ointments or cosmetics is preferably 0.0001-10.0% by weight, preferably 0.0005-10.0% by weight in dry weight. At concentrations below 0.0001% by weight, no significant effect could be expected. At concentrations of 10% by weight or more, there was no apparent increase in effect as the content increased.

다음으로 실시예에 의거하여 본 발명을 자세히 설명하고자 하며, 본 발명이 실시예에만 한정되는 것이 아님을 밝혀둔다.Next, the present invention will be described in detail with reference to Examples, and the present invention is not limited only to Examples.

실시예 1 - 18Examples 1-18

시판되는 지정, 와송 및 신곡을 구입하여 분쇄한 분쇄물 100 g을 물이나 탄소수 1 - 4 개의 무수 또는 함수 저급 알콜, 에틸아세테이트 또는 아세톤 등의 용매 3 ℓ에 넣고, 냉각 콘덴서가 달린 환류 추출기에서 3 시간 끓여 추출한 다음, 100 메쉬 여과포로 여과한 후 잔사를 같은 방법으로 1 회 더 추출하였다. 각각의 추출액을 합하여 상온에서 화트만(Whatman) 2 번 여과지로 여과하여 불용성 물질을 제거한 후, 냉각 콘덴서가 달린 증류 장치에서 60℃로 감압 농축한 후 동결 건조하여 [표 1]과 같이 각각의 건조 중량을 얻었다.100 g of a commercially available designation, vortex and new song, and pulverized crushed powder are placed in 3 l of a solvent such as water or anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, and 3 at reflux extractor with cooling condenser. The mixture was boiled for an hour, extracted with 100 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, followed by freeze drying, and drying each as shown in [Table 1]. The weight was obtained.

[표 1] 추출 용매 및 추출물의 건조 중량[Table 1] Dry weight of extraction solvent and extract

추출용매Extraction solvent 추출물의 건조중량Dry weight of extract 지정appointed 와송Wasong 신곡new song 80% 메탄올80% methanol 13 g (실시예 1)13 g (Example 1) 7 g (실시예 7)7 g (Example 7) 22 g (실시예 13)22 g (Example 13) 정제수Purified water 12 g (실시예 2)12 g (Example 2) 7 g (실시예 8)7 g (Example 8) 20 g (실시예 14)20 g (Example 14) 85% 에탄올85% ethanol 12 g (실시예 3)12 g (Example 3) 8 g (실시예 9)8 g (Example 9) 23 g (실시예 15)23 g (Example 15) 70% 아세톤70% acetone 8 g (실시예 4)8 g (Example 4) 5 g (실시예 10)5 g (Example 10) 14 g (실시예 16)14 g (Example 16) 에틸아세테이트Ethyl acetate 7 g (실시예 5)7 g (Example 5) 5 g (실시예 11)5 g (Example 11) 11 g (실시예 17)11 g (Example 17) 포화노말부탄올Saturated normal butanol 10 g (실시예 6)10 g (Example 6) 7 g (실시예 12)7 g (Example 12) 18 g (실시예 18)18 g (Example 18)

실험예 1Experimental Example 1

상기 실시예 1 - 18에 따라 얻어진 각 추출물들을 쥐의 멜라노마 세포(B-16 mouse melanoma cell)의 배양액에 첨가하여 세포 수준에서의 미백 효과를 실험하였 다(Lotan R., Lotan D. Cancer Res. 40:3345-3350, 1980). 실시예 1 - 18에 따라 얻어진 각 추출물들을 최종 농도가 0.005%가 되도록 하여 하이드로퀴논 수용액과 함께 각각 B-16 멜라노마 세포의 배양 배지에 첨가하여 3 일간 배양한 후 세포들을 트립신(trypsin) 처리하여 배양 용기로부터 떼어내 원심 분리한 후 멜라닌을 추출하였다. 이것에 수산화 나트륨 용액(1N 농도) 1 ㎖를 가하여 10 분간 끓여 멜라닌을 녹이고 분광 광도계를 이용, 400 나노미터(nm)에서 흡광도를 측정하여 생성된 멜라닌의 양을 단위 세포수(106 cells)당 흡광도로 나타내는 방법으로 수행하였으 며, 대조군에 대한 상대적인 멜라닌 생성량을 저해율(%)로 계산하고 결과를 [표 2]에 정리하였다.Each extract obtained according to Examples 1 to 18 was added to the culture solution of mouse B-16 mouse melanoma cells to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res 40: 3345-3350, 1980). Each of the extracts obtained according to Examples 1 to 18 were added to a culture medium of B-16 melanoma cells with hydroquinone aqueous solution, each of which was brought to a final concentration of 0.005%, followed by incubation for 3 days, followed by trypsin treatment. The melanin was extracted after centrifugation and removal from the culture vessel. To this, 1 ml of sodium hydroxide solution (1N concentration) was added, boiled for 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nanometer (nm) using a spectrophotometer to determine the amount of melanin produced per unit cell (10 6 cells) It was carried out by the method represented by the absorbance, the relative melanin production relative to the control group was calculated as the inhibition rate (%) and the results are summarized in [Table 2].

[표 2]의 결과에서 볼 수 있듯이, 실시예 1 - 18에 따라 얻어진 각 시료들은 전혀 정제되지 않은 추출물임을 감안하면 기존에 알려진 미백 물질인 하이드로퀴논과 비교할때 배양된 쥐의 멜라노마 세포에 대하여 매우 강력한 멜라닌 생성 억제능이 있음을 알 수 있다.As can be seen from the results in Table 2, considering that each sample obtained according to Examples 1-18 is an extract that is not purified at all, compared to the previously known whitening substance hydroquinone for the mouse melanoma cells cultured It can be seen that there is a very strong melanin production inhibitory ability.

[표 2]TABLE 2

지정, 와송 및 신곡 추출물의 세포 수준에서의 멜라닌 생성 저해 효과Inhibitory Effect of Melanin Production at the Cell-Level of Appointment, Wasong and Divine Extracts

시료sample 멜라닌 생성Melanin production 저해율 (%)Inhibition Rate (%) 대조군 (무첨가)Control group (no addition) 0.045 ± 0.0040.045 ± 0.004 -- 실시예 1 (최종농도 0.005%)Example 1 (final concentration 0.005%) 0.028 ± 0.0030.028 ± 0.003 38%38% 실시예 2 (최종농도 0.005%)Example 2 (final concentration 0.005%) 0.030 ± 0.0030.030 ± 0.003 33%33% 실시예 3 (최종농도 0.005%)Example 3 (final concentration 0.005%) 0.028 ± 0.0020.028 ± 0.002 38%38% 실시예 4 (최종농도 0.005%)Example 4 (final concentration 0.005%) 0.025 ± 0.0020.025 ± 0.002 44%44% 실시예 5 (최종농도 0.005%)Example 5 (final concentration 0.005%) 0.025 ± 0.0030.025 ± 0.003 44%44% 실시예 6 (최종농도 0.005%)Example 6 (final concentration 0.005%) 0.024 ± 0.0030.024 ± 0.003 47%47% 실시예 7 (최종농도 0.005%)Example 7 (final concentration 0.005%) 0.033 ± 0.0040.033 ± 0.004 27%27% 실시예 8 (최종농도 0.005%)Example 8 (final concentration 0.005%) 0.035 ± 0.0020.035 ± 0.002 22%22% 실시예 9 (최종농도 0.005%)Example 9 (final concentration 0.005%) 0.032 ± 0.0020.032 ± 0.002 29%29% 실시예 10 (최종농도 0.005%)Example 10 (final concentration 0.005%) 0.028 ± 0.0020.028 ± 0.002 38%38% 실시예 11 (최종농도 0.005%)Example 11 (final concentration 0.005%) 0.026 ± 0.0020.026 ± 0.002 42%42% 실시예 12 (최종농도 0.005%)Example 12 (final concentration 0.005%) 0.021 ± 0.0020.021 ± 0.002 53%53% 실시예 13 (최종농도 0.005%)Example 13 (final concentration 0.005%) 0.028 ± 0.0030.028 ± 0.003 38%38% 실시예 14 (최종농도 0.005%)Example 14 (final concentration 0.005%) 0.029 ± 0.0040.029 ± 0.004 35%35% 실시예 15 (최종농도 0.005%)Example 15 (final concentration 0.005%) 0.026 ± 0.0020.026 ± 0.002 42%42% 실시예 16 (최종농도 0.005%)Example 16 (final concentration 0.005%) 0.024 ± 0.0020.024 ± 0.002 47%47% 실시예 17 (최종농도 0.005%)Example 17 (final concentration 0.005%) 0.024 ± 0.0020.024 ± 0.002 47%47% 실시예 18 (최종농도 0.005%)Example 18 (final concentration 0.005%) 0.023 ± 0.0020.023 ± 0.002 49%49% 하이드로퀴논 (최종농도 0.0001%)Hydroquinone (final concentration 0.0001%) 0.025 ± 0.0020.025 ± 0.002 44%44%

* 반복수 = 3                                                     * Number of iterations = 3

실험예 2Experimental Example 2

실험예 1과 같은 방법으로 배양된 쥐의 멜라노마 세포에 대하여 실시예 1, 7, 13의 추출물에 대하여 농도에 따른 멜라닌 생성 억제능을 조사하였으며 결과는 [표 3]과 같다.The melanin production inhibitory ability of the rat melanoma cells cultured in the same manner as in Experimental Example 1 was examined according to the concentrations of the extracts of Examples 1, 7, and 13, and the results are shown in [Table 3].

[표 3] 농도에 따른 세포 수준에서의 멜라닌 생성 저해 효과[Table 3] Inhibitory Effect of Melanin Production at Cell Level

시료sample 적용 농도 (%)Application concentration (%) 멜라닌 생성량Melanin production 저해율 (%)Inhibition Rate (%) 대조군 (무첨가)Control group (no addition) -- 0.045 ± 0.0040.045 ± 0.004 -- 실시예 1Example 1 최종농도 (0.0005%)Final concentration (0.0005%) 0.039 ± 0.0020.039 ± 0.002 13%13% 최종농도 (0.005%)Final concentration (0.005%) 0.028 ± 0.0030.028 ± 0.003 38%38% 최종농도 (0.05%)Final concentration (0.05%) 0.024 ± 0.0020.024 ± 0.002 47%47% 최종농도 (0.5%)Final concentration (0.5%) 0.020 ± 0.0010.020 ± 0.001 56%56% 실시예 7Example 7 최종농도 (0.0005%)Final concentration (0.0005%) 0.042 ± 0.0020.042 ± 0.002 7%7% 최종농도 (0.005%)Final concentration (0.005%) 0.033 ± 0.0040.033 ± 0.004 27%27% 최종농도 (0.05%)Final concentration (0.05%) 0.022 ± 0.0020.022 ± 0.002 51%51% 최종농도 (0.5%)Final concentration (0.5%) 0.017 ± 0.0010.017 ± 0.001 62%62% 실시예 13Example 13 최종농도 (0.0005%)Final concentration (0.0005%) 0.038 ± 0.0020.038 ± 0.002 16%16% 최종농도 (0.005%)Final concentration (0.005%) 0.028 ± 0.0030.028 ± 0.003 38%38% 최종농도 (0.05%)Final concentration (0.05%) 0.017 ± 0.0020.017 ± 0.002 62%62% 최종농도 (0.5%)Final concentration (0.5%) 0.014 ± 0.0010.014 ± 0.001 69%69% 하이드로퀴논Hydroquinone (0.0001%)(0.0001%) 0.025 ± 0.0020.025 ± 0.002 44%44%

* 반복수 = 3                                                       * Number of iterations = 3

[표 3]의 결과에서 볼 수 있듯이 지정, 와송 및 신곡 추출물은 배양된 쥐의 멜라노마 세포에 대하여 매우 강력한 멜라닌 생성 억제능을 가지고 있음을 알 수 있다. 하이드로퀴논은 저농도에서 강력한 멜라닌 생성 억제능을 가지지만 세포 독성이 심해 0.0001% 이상에서는 실험을 할 수 없는 것에 반하여, 본 발명에서의 추출물들은 0.05% 농도 이상에서도 세포 독성을 나타내지 않으면서 사용 가능한 최대량의 하이드로퀴논보다 높은 멜라닌 생성 억제 효과를 갖는다. 또한 지정, 와송 및 신곡 추출물이 단일 물질이 아니고 혼합 물질이라는 것을 고려할 때 매우 높은 멜라닌 생성 억제능을 가지고 있으므로, 기미나 주근깨 개선 또는 피부 미백에 매우 유용하게 사용될 수 있다.As can be seen from the results of Table 3, the designated, vortex and singok extracts can be seen to have a very strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is severe and can not be tested at 0.0001% or more, whereas the extract of the present invention is the maximum amount of hydro that can be used without showing cytotoxicity even at 0.05% or more concentration It has a higher melanin production inhibitory effect than quinone. In addition, given that the designation, seungsong and new song extract has a very high melanogenesis inhibitory ability, considering that it is not a single substance but a mixed substance, it can be very useful for improving blemishes and freckles or skin whitening.

이하 지정, 와송 및 신곡 추출물을 함유하는 화장료의 처방예를 제조예에 의해 나타내었다.Hereinafter, the preparation example of the cosmetics containing the designation, vortex, and new song extract was shown by the preparation example.

제조예 1 - 3 및 비교예 1Preparation Examples 1-3 and Comparative Example 1

지정, 와송 및 신곡 추출물을 함유하는 피부 외용 연고 처방예는 다음과 같다.Examples of prescription ophthalmic ointments containing dermatitis, zinnia and new song extract are as follows.

지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.Designated, Wasong, and Singok extracts refer to those of Examples 5, 11, and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 1Comparative Example 1 1One 22 33 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 디에틸 세바케이트 - 경납 - 폴리옥시에틸렌올레일에테르 포스페이트 - 벤조산 나트륨 - 바셀린-Extract Designation Extract Wasong Extract New Song Extract-Diethyl Sebacate-Light Lead-Polyoxyethylene Oleyl Ether Phosphate-Sodium Benzoate-Vaseline 10 - - 8 5 6 적량 나머지 10--8 5 6 Proper Rest - 10 - 8 5 6 적량 나머지 -10-8 5 6 Proper Rest - - 10 8 5 6 적량 나머지 --10 8 5 6 Proper remainder - - - 8 5 6 적량 나머지 ---8 5 6 Proper Rest

제조예 4 - 6 및 비교예 2Preparation Examples 4-6 and Comparative Example 2

지정, 와송 및 신곡 추출물을 함유하는 화장료 중 크림의 처방예는 다음과 같다.Prescription examples of creams in cosmetics containing designated, Wasong and new song extracts are as follows.

여기서 지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.In this case, the designated, wasong and singok extract refers to those of Examples 5, 11 and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 2Comparative Example 2 44 55 66 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 스테아린산 - 세탄올 - 수산화칼륨 - 글리세린 - 프로필렌글리콜 - 방부제 - 향 - 정제수-Extract Designated Extract Wasong Extract New Song Extract-Stearic Acid-Cetanol-Potassium Hydroxide-Glycerin-Propylene Glycol-Preservative-Fragrance-Purified Water 1.0 - - 15.0 1.0 0.7 5.0 3.0 적량 적량 나머지 1.0--15.0 1.0 0.7 5.0 3.0 Proper quantity remaining - 1.0 - 15.0 1.0 0.7 5.0 3.0 적량 적량 나머지 -1.0-15.0 1.0 0.7 5.0 3.0 Proper quantity remaining - - 1.0 15.0 1.0 0.7 5.0 3.0 적량 적량 나머지 --1.0 15.0 1.0 0.7 5.0 3.0 appropriate amount remaining - - - 15.0 1.0 0.7 5.0 3.0 적량 적량 나머지 ---15.0 1.0 0.7 5.0 3.0 Proper quantity remaining

제조예 7 - 9 및 비교예 3Preparation Examples 7-9 and Comparative Example 3

지정, 와송 및 신곡 추출물을 함유하는 화장료 중 유연 화장수의 처방예는 다음과 같다.Prescription examples of the flexible lotion in cosmetics containing designated, Wasong and new song extracts are as follows.

여기서 지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.In this case, the designated, wasong and singok extract refers to those of Examples 5, 11 and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 3Comparative Example 3 77 88 99 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 에탄올 - 폴리라우린산폴리옥시에틸렌 소르비탄 - 파라옥시안식향산메칠 - 글리세린 - 1,3-부틸렌글리콜 - 향 - 색소 - 정제수-Extract Designated Extract Wasong Extract New Song Extract-Ethanol-Polylauric acid Polyoxyethylene Sorbitan-Paraoxybenzoic Acid Methyl-Glycerin-1,3-Butylene Glycol-Fragrance-Color-Purified Water 0.2 - - 10.0 1.0 0.2 5.0 6.0 적량 적량 나머지 0.2--10.0 1.0 0.2 5.0 6.0 Proper quantity remainder - 0.2 - 10.0 1.0 0.2 5.0 6.0 적량 적량 나머지 -0.2-10.0 1.0 0.2 5.0 6.0 Proper quantity remaining - - 0.2 10.0 1.0 0.2 5.0 6.0 적량 적량 나머지 --0.2 10.0 1.0 0.2 5.0 6.0 Proper quantity remaining - - - 10.0 1.0 0.2 5.0 6.0 적량 적량 나머지 ---10.0 1.0 0.2 5.0 6.0 Proper quantity remaining

제조예 10 - 12 및 비교예 4Preparation Examples 10-12 and Comparative Example 4

지정, 와송 및 신곡 추출물을 함유하는 화장료 중 영양 화장수의 처방예는 다음과 같다.Prescription examples of nourishing lotion in cosmetics containing designated, Wasong and new song extracts are as follows.

여기서 지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.In this case, the designated, wasong and singok extract refers to those of Examples 5, 11 and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 4Comparative Example 4 1010 1111 1212 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 와셀린 - 세스퀴올레인산소르비탄 - 폴리옥시에틸렌올레일에틸 - 파라옥시안식향산메칠 - 프로필렌글리콜 - 에탄올 - 카르복시비닐폴리머 - 수산화칼륨 - 색소 - 향 - 정제수-Extract Designated Extract Wasong Extract New Song Extract-Waselin-Sesquioleine Sorbitane-Polyoxyethylene Oleyl Ethyl-Paraoxybenzoic Acid Methyl-Propylene Glycol-Ethanol-Carboxy Vinyl Polymer-Potassium Hydroxide-Color-Flavor-Purified Water 0.1 - - 2.0 0.8 1.2 적량 5.0 3.2 18.0 0.1 적량 적량 나머지 0.1--2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining - 0.1 - 2.0 0.8 1.2 적량 5.0 3.2 18.0 0.1 적량 적량 나머지 -0.1-2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining - - 0.1 2.0 0.8 1.2 적량 5.0 3.2 18.0 0.1 적량 적량 나머지 --0.1 2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining - - - 2.0 0.8 1.2 적량 5.0 3.2 18.0 0.1 적량 적량 나머지 ---2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining

제조예 13 - 15 및 비교예 5Preparation Examples 13-15 and Comparative Example 5

지정, 와송 및 신곡 추출물을 함유하는 화장료 중 팩의 처방예는 다음과 같다.Prescription examples of packs in cosmetics containing designated, Wasong and new song extracts are as follows.

여기서 지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.In this case, the designated, wasong and singok extract refers to those of Examples 5, 11 and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 5Comparative Example 5 1313 1414 1515 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 글리세린 - 프로필렌글리콜 - 폴리비닐알콜 - 에탄올 - 폴리옥시에틸렌올레일에칠 - 파라옥시안식향산메칠 - 향 - 색소 - 정제수-Extract Designated Extract Wasong Extract New Song Extract-Glycerin-Propylene Glycol-Polyvinyl Alcohol-Ethanol-Polyoxyethylene Oleethyl-Paraoxybenzoic Acid Methyl-Flavor-Color-Purified Water 0.5 - - 5.0 4.0 15.0 8.0 1.0 0.2 적량 적량 나머지 0.5--5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remainder - 0.5 - 5.0 4.0 15.0 8.0 1.0 0.2 적량 적량 나머지 -0.5-5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remaining - - 0.5 5.0 4.0 15.0 8.0 1.0 0.2 적량 적량 나머지 --0.5 5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remaining - - - 5.0 4.0 15.0 8.0 1.0 0.2 적량 적량 나머지 ---5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remaining

제조예 16 - 18 및 비교예 6Preparation Examples 16-18 and Comparative Example 6

지정, 와송 및 신곡 추출물을 함유하는 화장료 중 에센스의 처방예는 다음과 같다.Prescription examples of essences in cosmetics containing designated, Wasong and new song extracts are as follows.

여기서 지정, 와송 및 신곡 추출물은 각각 실시예 5, 11, 17의 것을 말한다.In this case, the designated, wasong and singok extract refers to those of Examples 5, 11 and 17, respectively.

조성물Composition 제조예 (중량%)Preparation Example (wt%) 비교예 6Comparative Example 6 1616 1717 1818 - 추출물 지정 추출물 와송 추출물 신곡 추출물 - 프로필렌글리콜 - 글리세린 - 히아루론산나트륨수용액 (1%) - 에탄올 - 폴리옥시에틸렌겨와피마자유 - 파라옥시안식향산메칠 - 향 - 정제수-Extract Designated Extract Wasong Extract New Song Extract-Propylene Glycol-Glycerine-Sodium Hyaluronate (1%)-Ethanol-Polyoxyethylene Bran Castor Oil-Paraoxybenzoic Acid Methyl-Flavor-Purified Water 5 - - 10.0 10.0 5.0 5.0 1.0 0.1 적량 나머지 5--10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest - 5 - 10.0 10.0 5.0 5.0 1.0 0.1 적량 나머지 -5-10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest - - 5 10.0 10.0 5.0 5.0 1.0 0.1 적량 나머지 --5 10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest - - - 10.0 10.0 5.0 5.0 1.0 0.1 적량 나머지 ---10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest

실험예 3Experimental Example 3

건강한 남녀 20 명씩을 선정하여 양팔의 하박부에 직경 7 mm 크기의 구멍이 6 개씩 2 줄로 파인 알루미늄 호일을 붙이고, 팔에서 10 cm 떨어진 거리에서 ORIEL solar simulator 1000W를 사용하여 60 mJ/cm2의 광량을 조사하였다. 조사 전에 70% 에탄올 수용액으로 조사 부위를 잘 세척하였다. 조사하기 3 일 전부터 조사후 3 주째까지 1 일 2 회씩 제조예 1 - 18 및 비교예 1 - 6에 따라 제조된 지정, 와송 및 신곡 추출물을 함유한 시료와 각각의 추출물이 함유되지 않은 기제를 한 쌍으로 같은 줄에 도포하였다(제조예 13, 14, 15 및 비교예 5의 팩 처방의 경우 도포한 다음 15 분 후에 떼어내었다). 각각에 대하여 제조예와 비교예의 색소 침착도를 육안으로 판정하고, 제조예가 비교예에 비해 색소 침착을 억제한 정도를 뚜렷한 효과, 효과 있음, 차이없음의 3 단계로 평가하였으며, 그 결과는 [표 4]와 같다.Select 20 healthy men and women, attach two aluminum foils with 6 holes of 7 mm diameter to the lower hind limbs of each arm, and emit 60 mJ / cm 2 of light using ORIEL solar simulator 1000W at a distance of 10 cm from the arm. Was investigated. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. Twice a day twice a day from 3 days before irradiation to 3 weeks after irradiation, containing samples prepared according to Preparation Examples 1-18 and Comparative Examples 1-6, and extracts containing the extracts and bases free of each extract. It was applied in the same row in pairs (for the pack formulations of Preparation Examples 13, 14, 15 and Comparative Example 5, they were removed 15 minutes after application). For each, the degree of pigmentation of the preparation example and the comparative example was visually determined, and the degree to which the preparation example suppressed the pigmentation compared to the comparative example was evaluated in three stages of distinct effects, effects, and no differences, and the results are shown in [Table] 4].

[표 4] TABLE 4

지정, 와송 및 신곡 추출물을 함유한 연고 및 화장료의 색소 침착 저해 효과Pigmentation Inhibitory Effects of Ointments and Cosmetics Containing Aphid, Wasong and Singok Extracts

실험 물질Experimental substance 뚜렷한 효과 (명)Distinct Effect (People) 효과있음 (명)Effective (persons) 차이없음 (명)No difference (persons) 제조예 1Preparation Example 1 44 1010 66 제조예 2Preparation Example 2 55 99 66 제조예 3Preparation Example 3 55 1010 55 제조예 4Preparation Example 4 33 88 99 제조예 5Preparation Example 5 22 1111 77 제조예 6Preparation Example 6 22 1010 88 제조예 7Preparation Example 7 22 88 1010 제조예 8Preparation Example 8 22 99 99 제조예 9Preparation Example 9 22 1010 88 제조예 10Preparation Example 10 22 88 1010 제조예 11Preparation Example 11 33 77 1010 제조예 12Preparation Example 12 22 99 99 제조예 13Preparation Example 13 22 99 99 제조예 14Preparation Example 14 33 77 1010 제조예 15Preparation Example 15 22 77 1111 제조예 16Preparation Example 16 33 77 1010 제조예 17Preparation Example 17 33 88 99 제조예 18Preparation Example 18 44 88 88

위의 [표 4]의 결과에서 볼 수 있듯이, 제조예 1 - 18에 따라 제조된 지정, 와송 및 신곡 추출물 함유 미백 연고 및 화장료는 피시험자 20 명 중에서 최소 10 명 이상에 대하여 미백 효과를 나타내었으며, 특히 많은 양의 지정, 와송 및 신곡 추출물이 적용된 제조예 1, 2, 3과 비교예 1의 비교 실험에서는 70%가 색소 침착 저해 효과를 보이고, 피부내에서 어떤 부작용도 나타내지 않아 지정, 와송 및 신곡 추출물이 안전하고 효과가 매우 뛰어난 기미나 주근깨 개선 또는 피부 미백용 화장료 임을 알 수 있다.As can be seen from the results of [Table 4], the whitening ointment and the cosmetic containing the designated, hawsong and new song extract prepared according to Preparation Examples 1-18 showed a whitening effect on at least 10 of 20 subjects. In particular, in the comparative experiments of Preparation Examples 1, 2, 3 and Comparative Example 1, in which a large amount of designated, Wasong and Singok extracts were applied, 70% showed pigmentation inhibitory effect and did not show any side effects in the skin. Singok extract is safe and very effective in improving the appearance of freckles or skin whitening cosmetics.

상기 방법으로 제조된 지정, 와송 및 신곡 추출물은 쥐의 멜라노마 세포(B- 16 mouse melanoma cell)에서 매우 강력한 멜라닌 합성 저해 효과를 나타낸다. 또한 건조된 지정, 와송 및 신곡 추출물로 피부 외용 연고나 화장료를 제조하여 사람의 피부에 도포하였을 때, 기미, 주근깨 개선 및 피부 미백 효과가 매우 우수하다. Appointment, wasong and singok extract prepared by the above method has a very potent melanin synthesis inhibitory effect in the melanoma cells (B-16 mouse melanoma cells). In addition, when the dried ointment, wasong and singok extract to prepare an external skin ointment or cosmetics applied to the skin of the person, the blemish, freckles improvement and skin whitening effect is very excellent.                     

Claims (3)

지정, 와송 및 신곡 추출물로 이루어진 군으로부터 선택되어진 1 종 이상의 추출물을 유효 성분으로 함유하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening containing, as an active ingredient, at least one extract selected from the group consisting of designated, waxy and new song extracts. 제 1항에 있어서, 상기 피부 미백용 화장료 조성물은 지정, 와송 및 신곡 추출물을 건조 중량으로 0.0001 - 10.0 중량% 함유하는 것을 특징으로하는 피부 미백용 화장료 조성물.The cosmetic composition for skin whitening according to claim 1, wherein the cosmetic composition for skin whitening contains 0.0001-10.0% by weight of designated, vortex and singok extracts in dry weight. 제 1항에 있어서, 상기 지정, 와송 및 신곡 추출물은 지정, 와송 및 신곡을물, 탄소수 1 - 4의 저급 알콜, 에틸아세테이트 또는 아세톤으로 1 회 이상 추출하여 추출액을 얻고, 상기 추출액을 감압 증류한 후 동결 건조하거나 분사 건조하여 얻는 것을 특징으로하는 피부 미백용 화장료 조성물.The method of claim 1, wherein the extract, Waksong and new song extract is extracted at least one time, the extract, Waksong and new song with water, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone to obtain an extract, the extract is distilled under reduced pressure After freeze-drying or spray-dried cosmetic composition for skin whitening, characterized in that obtained.
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KR101238649B1 (en) 2010-11-24 2013-02-28 주식회사 한국화장품제조 A cosmetic composition comprising Massa Medicata Fermentata Extracthaving effect of anti-oxident, a preparing method thereof and a cosmetic using the same

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KR101877510B1 (en) * 2016-05-31 2018-07-11 (주)월드코스텍 Method for increasing production of orostachys japonicus and copposition containing extract of orostachys japonicus thereof
KR102017377B1 (en) * 2017-03-29 2019-09-02 인제대학교 산학협력단 Cosmetic composition for skin improvement Containing Orostachys japonicus extract
CN112656746B (en) * 2021-01-07 2023-11-28 美丽链接生物科技研究院(广东)有限公司 Whitening and freckle-removing cream containing blackberry lily root extract

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KR101238649B1 (en) 2010-11-24 2013-02-28 주식회사 한국화장품제조 A cosmetic composition comprising Massa Medicata Fermentata Extracthaving effect of anti-oxident, a preparing method thereof and a cosmetic using the same
CN102645497A (en) * 2011-05-16 2012-08-22 成都中医药大学 Method for inspecting quality of Gueldenstaedtia delavayi Franch.
CN102645497B (en) * 2011-05-16 2013-08-28 成都中医药大学 Method for inspecting quality of Gueldenstaedtia delavayi Franch.

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