KR100536086B1 - Mixture of pine tree leaves extract and barley germs leaves and health food containing the same - Google Patents
Mixture of pine tree leaves extract and barley germs leaves and health food containing the same Download PDFInfo
- Publication number
- KR100536086B1 KR100536086B1 KR10-2003-0067995A KR20030067995A KR100536086B1 KR 100536086 B1 KR100536086 B1 KR 100536086B1 KR 20030067995 A KR20030067995 A KR 20030067995A KR 100536086 B1 KR100536086 B1 KR 100536086B1
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- South Korea
- Prior art keywords
- extract
- mixture
- barley
- pine needle
- pine
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- 229930182817 methionine Natural products 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 150000002773 monoterpene derivatives Chemical class 0.000 description 1
- 235000002577 monoterpenes Nutrition 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 201000001119 neuropathy Diseases 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
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- 239000011570 nicotinamide Substances 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
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- 230000000144 pharmacologic effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
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- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 238000000746 purification Methods 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 229960004172 pyridoxine hydrochloride Drugs 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 230000001850 reproductive effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- YXJHJCDOUFKMBG-BMZHGHOISA-M riboflavin sodium Chemical compound [Na+].OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)[N-]C2=O YXJHJCDOUFKMBG-BMZHGHOISA-M 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- RXHIKAIVEMAPRU-JRIGQVHBSA-N sequiterpene Natural products C1=C(C)[C@@H](OC(C)=O)[C@H](O)[C@@]2(O)[C@H](C)CC[C@@H](C(C)=C)[C@H]21 RXHIKAIVEMAPRU-JRIGQVHBSA-N 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
본 발명은 솔잎 엑기스와 보리싹 엑기스 혼합액 또는 이의 분말 및 상기 혼합액 또는 분말을 유효성분으로 함유하는 건강 보조 식품에 관한 것으로, 보다 구체적으로는 솔잎 엑기스 70 내지 90 중량%, 보리싹 엑기스 10 내지 30 중량%로 혼합하여 제조되는 항암, 항세균, 항산화 효과가 뛰어난 혼합액 또는 이의 분말 및 상기 혼합액 또는 분말을 유효성분으로 함유하는 건강 보조 식품에 관한 것이다. 본 발명의 솔잎 엑기스 및 보리싹 엑기스 혼합액 또는 이의 분말은 주요 구성성분이 잘 보존되어 있고 항암, 항산화 및 항세균 효과가 뛰어나기 때문에 건강 보조 식품으로 유용하게 사용될 수 있다.The present invention relates to a pine needle extract and barley sprout extract mixture or powder thereof and a dietary supplement containing the mixture or powder as an active ingredient, more specifically 70 to 90% by weight pine needle extract, barley sprout extract 10 to 30 weight The present invention relates to an anticancer, an antibacterial, a mixed liquid having excellent antioxidant effect, or a powder thereof and a dietary supplement containing the mixed liquid or powder as an active ingredient. Pine needle extract and barley sprout extract mixture or powder thereof of the present invention can be usefully used as a dietary supplement because the main components are well preserved and excellent anti-cancer, antioxidant and antibacterial effect.
Description
본 발명은 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말 및 상기 혼합액 또는 이의 분말을 유효성분으로 함유하는 건강 보조 식품에 관한 것이다.The present invention relates to a mixed solution of pine needle extract and barley sprout extract or a powder thereof and a dietary supplement containing the mixed solution or powder thereof as an active ingredient.
소나무(pinus densiflora Siebold et Zuccarini)는 소나무과(Pinaceae)에 속하는 사록 침엽성 교목으로 우리나라 전국의 산에 야생하고 있다(김일혁, 약품식물학각론, 진명출판사, 1981, 71). 솔잎은 예로부터 건위, 보혈작용이 있고 중풍, 동맥경과, 고혈압, 당뇨병 등을 예방하는 효능이 있는 것으로 알려져 왔으며(김완희, 최신동의보감, 태평양 출판공사, 1983, 960) 선식이나 차로도 이용되고 있다.Pine tree (Pinus densiflora Siebold et Zuccarini) is a coniferous tree belonging to the Pinaceae family and is wild in the mountains of Korea (Kim Il-hyuk, Ph. Pine needles have long been known to be effective in preventing blood stroke, arteriosclerosis, hypertension, diabetes, etc. (Kim Wan-hee, Hyeon Dong Bogam, Pacific Publishing Co., 1983, 960).
솔잎의 활성 성분이며 휘발성 항생물질인 테르펜은 솔잎에 약 7-12%가량 들어 있고 다량의 이소프렌으로 구성되어 모노테르펜(monoterpene), 세퀴테르펜(sequiterpene), 디테르펜(diterpene)이라고 불리며, 항생, 항암, 혈압강하, 호르몬 분비촉진, 항산화 등과 같은 독특한 약리작용 효과가 있어 아로마테라피(aromatheraphy)에 사용되기도 한다. 솔잎은 필수아미노산 8종을 포함해 단백질 구성 아미노산 16종을 풍부하게 함유하고 있기 때문에 신체의 성장, 활력 증강에 효과가 크며 혈액의 흐름을 좋게 하고 호르몬 분비를 왕성하게 하는 특성을 가지고 있어 노화방지와 고지혈증 및 콜레스테롤 저하에 뛰어난 효과를 나타낸다.Terpene, an active ingredient of pine needles and volatile antibiotics, contains about 7-12% of pine needles and is composed of a large amount of isoprene, called monoterpene, sequiterpene, and diterpene. It also has a unique pharmacological effect, such as lowering blood pressure, stimulating hormone secretion, and antioxidants. Pine needles contain 16 essential amino acids, including 8 essential amino acids, which are very effective for body growth and vitality, improve blood flow and stimulate hormone secretion. Excellent effect on hyperlipidemia and cholesterol lowering.
솔잎의 유효성분 중에서 비타민 C는 혈액정화 및 항괴혈병성, 스트레스에 대한 저항력을 가지고, 비타민 A는 점막을 튼튼히 하는 기능을 하여 야맹증치료에 사용되고, 수지 및 탄닌은 소화기의 기능을 돕는다. 또한 알콜 및 에스테르는 체내의 노폐물을 배출시켜 신진대사를 촉진시키고, 석회질을 용해하는 성분을 가지고 있어 동맥경화증에 유효하며, 철분은 빈혈치료에 효과적이고, 엽록소(클로로필)는 축적된 콜레스테롤을 감소시키고, 클리코기닌은 혈당강화작용이 있어 당뇨에 효과적이다. 또한, 아피에틴산은 아편과 니코틴 해독, 폐결핵이나 늑막염, 위장질환에 좋고, 비타민 C와 철분이 풍부해 빈혈에 효능이 있고, 테르펜은 불포화지방산, 동맥경화의 원인이 되는 콜레스테롤을 용해시켜 혈액순환을 촉진시키며, 비타민P(루틴)은 모세혈관을 강화시켜 노화방지에 도움을 준다.Among the active ingredients of pine needles, vitamin C has a blood purification and anti-neuropathy, and resistance to stress, vitamin A functions to strengthen the mucous membranes, and is used to treat night blindness, and resin and tannin help the digestive function. In addition, alcohols and esters promote body metabolism by releasing waste products in the body, and are effective in treating atherosclerosis due to the components of calcification. Iron is effective in treating anemia, and chlorophyll (chlorophyll) reduces accumulated cholesterol. In addition, glycogenin has a potentiating effect on diabetes and is effective in diabetes. In addition, apienic acid is good for detoxifying opiates and nicotine, tuberculosis and pleurisy, and gastrointestinal diseases.It is rich in vitamin C and iron, which is effective for anemia. Vitamin P (rutin) helps to prevent aging by strengthening capillaries.
또한, 보리잎은 예전부터 약제로 사용되어 왔으며, 비타민, 효소, 엽록소 등 영양소가 풍부하여 몸안의 독을 해독시키는 효과가 있다. 특히 간염이나 간경화증 치료에 사용되고 있으며, 미국, 일본 등에서도 어린 보리잎을 동결건조하여 가루로 만들어 건강식품으로 개발되었다. 보리잎의 영양소 분석에 의하면, 보리잎은 채소중에서도 미네랄이 가장 풍부하며, 시금치보다 칼슘은 11배, 마그네슘은 3배에 달한다. 보리잎의 천연엽록소는 인체와 유사한 혈색소 분자구조식을 가지고 있다. 또한, 보리잎은 다량의 비타민을 함유하고 있는데 비타민 B1은 우유의 30배, 비타민C는 6.5배에 달하는 우수한 식품이다.In addition, barley leaves have been used as a drug for a long time, rich in nutrients such as vitamins, enzymes, chlorophyll and the like has the effect of detoxifying the poison in the body. In particular, it is used for the treatment of hepatitis and cirrhosis, and in the United States and Japan, young barley leaves have been lyophilized and developed into a health food. Nutrient analysis of barley leaves shows that barley leaves are the most abundant in minerals, with 11 times more calcium and 3 times more magnesium than spinach. Natural chlorophyll of barley leaves has a molecular structure of hemoglobin similar to the human body. In addition, barley leaves contain a large amount of vitamins, vitamin B 1 is 30 times that of milk, vitamin C is 6.5 times the excellent food.
이에, 본 발명자들은 솔잎 및 보리싹으로부터 주요 구성 성분의 손실 및 변화없이 효율적으로 각각의 엑기스를 제조하고 이를 혼합한 혼합액 또는 이의 분말을 이용하여 건강 보조 식품을 제조함으로써 본 발명을 완성하였다.Accordingly, the present inventors have completed the present invention by efficiently preparing each extract from the pine needles and barley sprouts without loss and change of the main components and by using the mixed solution or powder thereof to prepare a dietary supplement.
본 발명의 목적은 항암, 항산화 및 항세균 효과가 뛰어난 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말 및 상기 혼합액 또는 이의 분말을 유효성분으로 함유하는 건강 보조 식품을 제공하는 것이다. It is an object of the present invention to provide a mixed solution or powder thereof of pine needle extract and barley sprout extract having excellent anticancer, antioxidant and antibacterial effects, and a dietary supplement containing the mixed solution or powder thereof as an active ingredient.
상기 목적을 달성하기 위해, 본 발명은 항암, 항산화 및 항세균 효과가 뛰어난 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말을 제공한다.In order to achieve the above object, the present invention provides a mixed liquid or powder thereof of pine needle extract and barley sprout extract excellent in anticancer, antioxidant and antibacterial effect.
또한, 본 발명은 상기 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말을 유효성분으로 함유하는 건강 보조 식품을 제공한다.In addition, the present invention provides a dietary supplement containing the mixed solution of the pine needle extract and barley sprout extract or powder thereof as an active ingredient.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 항암, 항산화 및 항세균 효과가 뛰어난 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말을 제공한다.The present invention provides a mixed liquid or powder thereof of pine needle extract and barley sprout extract excellent in anticancer, antioxidant and antibacterial effects.
상기 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말에 있어서, 솔잎 엑기스 : 보리싹 엑기스 = 70 내지 90 중량% : 10 내지 30 중량%인 것이 바람직하며, 솔잎 엑기스 : 보리싹 엑기스 = 80 중량% : 20 중량%인 것이 더욱 바람직하다.In the mixed solution of the pine needle extract and barley sprout extract or powder thereof, pine needle extract: barley sprout extract = 70 to 90% by weight: preferably 10 to 30% by weight, pine needle extract: barley sprout extract = 80% by weight: 20 It is more preferable that it is weight%.
상기에서 솔잎 엑기스는 Pine needle extract from the above
1) 솔잎을 세척하는 단계; 및1) washing the pine needles; And
2) 세척한 솔잎을 분쇄하여 착즙하는 단계를 포함하여 제조되는 것이 바람직하다.2) It is preferable that the prepared pine needles are pulverized and then juiced.
상기 단계 1에 있어서, 솔잎은 아황산가스의 오염을 최소화하기 위해 해발 350 m 이상에서 자란 청정지역의 건강한 소나무를 선택하여 영양상태가 좋은 가지부분을 채취한 후 싱싱하고 푸른잎만 선별하는 것이 바람직하다.In the step 1, it is preferable that pine needles are selected from healthy pine trees in a clean area grown at more than 350 m above sea level to minimize contamination of sulfurous acid gas. .
상기에서 선별된 솔잎의 불순물이 녹아 나오도록 살균된 맑은물에 담근 후 맑은 물이 나올 때까지 3-4회 세척하고 다시 숯을 우려낸 물로 1회 세척하는 것이 바람직하다.After dipping in the sterilized clear water to dissolve the impurities of the pine needles selected above, it is preferable to wash 3-4 times until clear water comes out, and then washed once with water soaked with charcoal.
상기 단계 2에 있어서, 세척한 솔잎을 분쇄하여 착즙하는 단계는 단계 1에서 세척된 솔잎 표면의 수분이 완전히 제거될 때까지 탈수한 뒤 녹즙기로 분쇄하여 착즙하는 것을 특징으로 한다. 녹즙기로 솔잎을 분쇄하는 과정에서 가장 중요한 점은 녹즙 도중 열이 발생하지 않도록 하며 압착점이 정밀하도록 하는 것이다.In the step 2, the step of crushing the pine needles washed, characterized in that the dehydration until the moisture of the surface of the pine needles washed in step 1 is completely removed and then pulverized with a juicer to juice. The most important thing in the process of grinding pine needles with green juice is to prevent heat from being generated during green juice and to make the pressing point precise.
또한, 상기에서 보리싹 엑기스는In addition, the barley sprout extract from the above
1) 보리싹을 세척하는 단계; 및1) washing the barley sprouts; And
2) 세척한 보리싹을 분쇄하여 착즙하는 단계를 포함하여 제조되는 것이 바람직하다.2) It is preferable that the prepared barley sprouts are pulverized and juiced.
본 발명에서는 상기 방법을 통해 솔잎 엑기스와 보리싹 엑기스의 혼합액을 제조하고 이를 이용해 분말을 제조하였으며, 이를 "송맥프라임"이라 명명하였다. 송맥프라임을 이용하여 주요 성분 및 효과를 분석한 결과, 솔잎 및 보리싹의 주요 성분이 잘 보존되고 그 함유량도 우수함을 알 수 있었으며(표 1, 표 2 및 표 3 참조), 항암(도 1 및 표 4 참조), 항세균(도 2 참조), 항산화(표 5 참조) 효과가 뛰어남을 알 수 있었다.In the present invention, a mixed solution of pine needle extract and barley sprout extract was prepared through the above method, and a powder was prepared using the same, which was named "songmac prime". As a result of analyzing the main components and effects using pine bark prime, it was found that the main components of pine needles and barley sprouts were well preserved and their contents were excellent (see Table 1, Table 2 and Table 3). Table 4), antibacterial (see Fig. 2), antioxidant (see Table 5) was found to be excellent.
또한, 본 발명은 상기 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 이의 분말을 유효성분으로 함유하는 건강 보조 식품을 제공한다.In addition, the present invention provides a dietary supplement containing the mixed solution of the pine needle extract and barley sprout extract or powder thereof as an active ingredient.
본 발명의 방법으로 제조된 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 분말은 항암, 항산화 및 항세균 효과가 뛰어나며 주요 성분을 성분 변화없이 다량 함유하고 있어 이를 이용하여 건강을 증진시키기 위한 건강 보조 식품을 제조할 수 있다. 본 발명의 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 분말을 이용하여 건강 보조 식품을 제조할 경우, 상기 솔잎 엑기스와 보리싹 엑기스의 혼합액 또는 분말을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효 성분의 혼합양은 그의 사용 목적(예방, 건강 또는 치료적 처치)에 따라 적합하게 결정될 수 있다.The mixed solution or powder of pine needle extract and barley sprout extract prepared by the method of the present invention is excellent in anti-cancer, antioxidant and antibacterial effects, and contains a large amount of main ingredients without changing the ingredients, thereby preparing health supplements for improving health. can do. When preparing a dietary supplement using a mixed solution or powder of pine needle extract and barley sprout extract of the present invention, the mixed solution or powder of the pine needle extract and barley sprout extract can be added as it is or used with other food or food ingredients, It can be suitably used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment).
상기 식품의 종류에는 특별한 제한은 없다. 상기 물질을 첨가할 수 있는 식품의 예로는 생식, 육류, 소세지, 빵, 쵸코렛, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강 보조 식품을 모두 포함한다.There is no particular limitation on the kind of food. Examples of the food to which the substance may be added include raw foods, meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinks, teas, Drinks, alcoholic beverages and vitamin complexes, and the like all include dietary supplements in the conventional sense.
이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of examples.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.
<실시예 1> 솔잎 엑기스의 제조Example 1 Preparation of Pine Needle Extract
아황산가스의 오염을 최소화하기 위해 해발 350 m 이상에서 자란 청정지역의 건강한 소나무를 선택하여 영양상태가 좋은 가지부분을 채취한 후 싱싱하고 푸른잎만 선별하였다. 상기 선별된 솔잎의 불순물이 녹아 나오도록 자오살균기(영진무역, AOP300)로 살균된 맑은물에 담근 후 맑은 물이 나올때까지 3-4회 세척하였다. 상기 세척된 솔잎을 숯을 우려낸 물로 다시 1회 세척하였다. 상기 세척된 솔잎 표면의 수분이 완전히 제거될 때까지 탈수하였다. 탈수된 솔잎을 녹즙기(인화정밀, 삼광무력공사, 명신상사)로 분쇄하여 착즙하였다. 본 발명에서 사용한 녹즙기는 재래식 녹즙기에 비해 성능이 향상된 것으로 분쇄기와 압축기를 따로 사용하고, 착즙시 전력 소모가 적으며, 착즙 부위가 FDA 승인 제품인 폴리카보네이트이고, 감속기어를 사용하기 때문에 소음과 진동이 적으며, 작업 과정이 단조로와 영양소의 손실이 적고, 부품이 세분화되어 있어 수리비용이 적게들며, 착즙액의 비율이 재래식보다 약 3배 이상 높은 장점이 있다. 상기 솔잎의 분쇄 과정 중에는 열이 발생하지 않게하여 압착점이 정밀하도록 하였다. 상기 분쇄로 착즙된 솔즙을 "솔잎 엑기스"라 명명하였으며, 결정이 생기지 않도록 -70℃ 이하의 냉동고에서 저장하였다. 상기 솔잎의 분쇄 과정 중에는 열이 발생하지 않게하여 압착점이 정밀하도록 하였다.To minimize contamination of sulfur dioxide, healthy pine trees were grown in a clean area over 350 m above sea level. After soaking the impurities of the selected pine needles soaked in clear water sterilized by a sterile sterilizer (Youngjin Trading, AOP300) and washed 3-4 times until clear water comes out. The washed pine needles were washed once again with water soaked with charcoal. Dehydration was performed until the water on the washed pine needle surface was completely removed. The dehydrated pine needles were pulverized with green juice (Fhwahwa Precision, Samkwang Armed Forces Corp., Myeongshin Corp.) and juiced. The juicer used in the present invention has an improved performance compared to conventional juicers, which uses a separate grinder and a compressor, consumes less power when juiced, and the juice part is a polycarbonate which is an FDA-approved product, and noise and vibration are reduced by using a reduction gear. It is less, the process is less forging and the loss of nutrients, the parts are broken down, the repair cost is low, the ratio of juice is about three times higher than conventional. During the crushing process of the pine needles so that heat does not occur so that the compression point is precise. The pine juice extracted by the grinding was named "pine leaf extract", and stored in a freezer at -70 ° C or lower to prevent crystals from forming. During the crushing process of the pine needles so that heat does not occur so that the compression point is precise.
<실시예 2> 보리싹 엑기스의 제조Example 2 Preparation of Barley Sprout Extract
건강한 보리 종자를 선별하여 농약이 살포되지 않고 오염되지 않은 토양을 선택해 배양판에 파종하였다. 파종 후 7 내지 10 ㎝ 정도 자란 어린 보리싹을 채취하였다. 상기 채취된 보리싹에서 이물질을 제거하고 맑은 물로 여러차례 깨끗이 세척하였다. 상기 세척된 보리싹은 표면의 수분이 완전히 제거될 때까지 탈수기로 탈수한 뒤 상기 실시예 1과 동일하게 분쇄하여 착즙한 후 이를 "보리싹 엑기스"라 명명하였다. 제조된 보리싹 엑기스는 -70℃ 냉동고에 저장하였다.Healthy barley seeds were selected and selected for unpolluted, unpolluted soil and sown in culture plates. After sowing, young barley shoots grown about 7 to 10 cm were collected. The foreign material was removed from the barley shoots and washed several times with clear water. The washed barley sprouts were dehydrated with a dehydrator until the water on the surface was completely removed, and then pulverized and juiced in the same manner as in Example 1, which was named "barley sprout extract." Barley sprout extract was stored in the -70 ℃ freezer.
<실시예 3> 솔잎 엑기스 및 보리싹 엑기스의 혼합물 제조Example 3 Preparation of a Mixture of Pine Needle Extract and Barley Sprout Extract
상기 실시예 1에서 제조한 솔잎 엑기스 80 ㎖과 보리싹 엑기스 20 ㎖을 혼합한 후 습열 멸균된 유리용기에 담아 UV 처리하였다. 또한, 상기 혼합 및 처리된 상기 혼합액을 예열없이 동결 건조한 뒤 동결건조된 혼합액을 마쇄기로 분말화하였고, 이를 "송맥프라임(솔잎 엑기스와 보리싹 엑기스의 혼합물)"이라 명명하였다.80 ml of pine needle extract prepared in Example 1 and 20 ml of barley sprout extract were mixed and placed in a wet heat sterilized glass container for UV treatment. In addition, the mixed and treated mixture was freeze-dried without preheating, and the lyophilized mixed solution was powdered with a crusher, which was named "mixed pine needle extract (barley leaf extract and barley sprout extract)".
<실시예 4> 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)의 성분 조사Example 4 Component Investigation of a Mixture of Pine Needle Extract and Barley Sprout Extract (Songmac Prime)
<4-1> 일반 성분 조사<4-1> General ingredient investigation
1) 수분(moisture) : 상기 실시예 3에서 제조한 송맥프라임을 적외선 수분측정장치(MB45, OHAUS, U.S.A)를 이용해 105℃에서 가열 건조하여 무게 변화가 없는 상태를 수분 함량으로 결정하였다.1) Moisture (moisture): The dried truffles prepared in Example 3 were heated and dried at 105 ° C. using an infrared moisture measuring device (MB45, OHAUS, U.S.A) to determine the moisture content.
2) 조회분(crude ash) : 상기 실시예 3에서 제조한 송맥프라임을 회화로(F-4800, Thermolyne, U.S.A)에서 550℃로 회화하여 무게 변화가 없는 상태를 회분 함량으로 결정하였다.2) Crude ash (crude ash): The trellis prepared in Example 3 was calcined at 550 ° C. in an incineration furnace (F-4800, Thermolyne, U.S.A) to determine a state without weight change as ash content.
3) 조지방 : 상기 실시예 3에서 제조한 송맥프라임에 페트로리움 에테르(Petroleum ether)를 추출 용매로 첨가하고 지방 추출 장치(B-811, Bu"chi, Switzerland)를 이용하여 지방을 추출한 후 정량하여 그 무게를 조지방 함량으로 결정하였다.3) Crude fat: Petroleum ether (Petroleum ether) is added to the extracts prepared in Example 3 as an extraction solvent and the fat is extracted using a fat extraction device (B-811, Bu "chi, Switzerland) and quantified The weight was determined by the crude fat content.
4) 조단백질(crude protein) : 상기 실시예 3에서 제조한 송맥프라임에 황산, 분해 촉진제(K2SO4 3.5 g과 Se 3.5 ㎎)를 넣어 분해장치(K-424/B-324, Bu"chi, Switzerland)를 사용하여 가열하여 완전 분해한 후, 증류장치를 이용해 질소량을 측정하고, 측정된 질소 함량은 단백질 계수를 적용하여 단백질로 환산하였다.4) crude protein (crude protein): sulfuric acid, decomposition promoters (K 2 SO 4 3.5 g and Se 3.5 mg) in the pine malt prime prepared in Example 3, the decomposition device (K-424 / B-324, Bu "chi , Switzerland), and then completely decomposed by heating, and the nitrogen content was measured using a distillation apparatus, and the measured nitrogen content was converted into protein by applying a protein coefficient.
그 결과, 상기 실시예 3에서 제조한 송맥프라임에 포함된 수분은 3.50 ±0.46%, 조회분은 3.60 ±0.00%, 조지방은 13.50 ±0.14%, 조단백질은 5.59 ±0.07%이었다.As a result, the moisture contained in the pine malt prime prepared in Example 3 was 3.50 ± 0.46%, crude ash was 3.60 ± 0.00%, crude fat was 13.50 ± 0.14%, crude protein was 5.59 ± 0.07%.
<4-2> 특정 성분 조사<4-2> Specific ingredient investigation
1) 비타민 C: 상기 실시예 <4-1>에서 제조한 송맥프라임을 5% 메타인산으로 추출한 뒤 수욕조(water bath)에서 DNP(2,4-dinitrophenyl hydrazine) 비색법으로 비색 반응한 후, 520 nm에서 흡광도를 측정하여 정량하였다(마상조 등, 식품분석법, 유림문화사, 1994). 또한, 5% 메타인산으로 추출한 후, 헥산으로 비극성 성분을 제거한 후 이동상은 아세토니트릴(acetonitrile) 30%, 0.05M KH2PO4 70% 이소크라틱(isocratic)으로 유속은 1 ㎖/분, 컬럼은 NH2 컬럼, 검출은 254 nm 파장의 UV 검출기로 하여 HPLC(LC-10Avp, Shimadzu, Japan)로 측정하였다.1) Vitamin C: After extracting the pine bark prime prepared in Example <4-1> with 5% metaphosphoric acid and colorimetrically reacted with DNP (2,4-dinitrophenyl hydrazine) in a water bath (520), 520 Absorbance at nm was measured and quantified. After extraction with 5% metaphosphoric acid, the non-polar component was removed with hexane, and the mobile phase was acetonitrile 30%, 0.05M KH 2 PO 4 70% isocratic, and the flow rate was 1 ml / min, column The silver NH 2 column was detected by HPLC (LC-10Avp, Shimadzu, Japan) using a UV detector with a wavelength of 254 nm.
2) 엽록소: 85% 아세톤을 넣어 녹색이 없어질 때까지 추출하여 일정량으로 정용하였다. 이 중 일부를 분획여두(separating funnel)에서 에테르(ether)로 지용성 성분을 분획하였다. 에테르층을 물층으로 통과시켜 아세톤을 제거하였다. 상기 과정을 5-10회 반복하여 완전히 아세톤을 제거한 에테르층만을 모아 무수 아황산 나트륨으로 탈수시키고, 흡광 광도계로 엽록소의 양을 측정하였다. 이때, 파장을 660 및 642.5 nm로 하여 총 클로로필(Chlorophyll), 클로로필 a, 클로로필 b 함량을 산출하였다.2) Chlorophyll: 85% acetone was added until the green color disappeared to a certain amount. Some of them were fractionated fat-soluble components with ether in a separating funnel. Pass the ether layer through the water layer to remove acetone. The procedure was repeated 5-10 times to collect only the acetone-free ether layer and dehydrated with anhydrous sodium sulfite, and the amount of chlorophyll was measured with an absorbance photometer. At this time, the total chlorophyll, chlorophyll a, and chlorophyll b contents were calculated at wavelengths of 660 and 642.5 nm.
3) 무기성분 : 상기 실시예 <4-1>에서 회화한 시료를 1:1, 1:3 염산 용액으로 건고시켜 안정화한 다음, 증류수로 적정량을 정용하여 원자 흡수 흡광기(Atomic Absorption Spectrophotometer)(AA-6800, Shimadzu, Japan)로 각 원소에 대하여 측정하였다.3) Inorganic Components: After stabilizing the sample assimilated in Example <4-1> with 1: 1, 1: 3 hydrochloric acid solution, and then applying an appropriate amount with distilled water, an atomic absorption spectrophotometer ( AA-6800, Shimadzu, Japan) was measured for each element.
그 결과, 송맥프라임 100 g에 포함된 비타민 C, 엽록소 및 무기성분의 함량은 하기 표 1과 같다.As a result, the content of vitamin C, chlorophyll and inorganic components contained in 100 g of pine bark prime is shown in Table 1 below.
또한, 상기 측정된 자료를 기초로 하여 단백질, 지방, 칼슘 및 비타민 C의 함량을 다른 주요 식품과 비교한 결과, 대부분의 성분이 우유, 시금치, 쌀보리순 및 올보리순에 비해 높았다(표 2).In addition, on the basis of the measured data, the content of protein, fat, calcium and vitamin C was compared with other major foods, and most of the ingredients were higher than those of milk, spinach, rice barley, and olive barley (Table 2).
<4-3> 구성 아미노산 조사<4-3> Component amino acid investigation
상기 실시예 1에서 제조한 솔잎 엑기스와 6 N 염산을 1:15로 섞어 110℃ 항온기에서 22시간 동안 가수분해한 후 여과하였다. 여과한 여액을 감압농축하여 염산을 제거하고, 증류수로 2-3회 수세하여 감압농축하였다. 농축분은 pH 2.2 소듐 시트레이트 버퍼(sodium citrate buffer)에 정용하여 아미노산 분석기(LC-10A vp, Shimadzu, Japan)로 분석하였다(표 3).The pine needle extract prepared in Example 1 and 6N hydrochloric acid were mixed at 1:15, and hydrolyzed at 110 ° C. for 22 hours, followed by filtration. The filtrate was concentrated under reduced pressure to remove hydrochloric acid, washed with distilled water 2-3 times and concentrated under reduced pressure. The concentrate was analyzed by amino acid analyzer (LC-10A vp, Shimadzu, Japan) by applying to sodium citrate buffer pH 2.2 (Table 3).
그 결과, 본 발명에서 제조한 송맥프라임은 본래의 솔잎 성분 및 보리싹 성분을 잘 포함하고 있으며 성분의 변화없이 다량 함유하고 있음을 알 수 있었다.As a result, it was found that the pine bark prime prepared in the present invention contains the original pine needle component and barley sprout component well and contains a large amount without changing the components.
<실시예 5> 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)의 항암 효과 분석Example 5 Anticancer Effect Analysis of a Mixture of Pine Needle Extract and Barley Sprout Extract (Songmac Prime)
<5-1> 항암효과 1<5-1> anticancer effect 1
인간 난소암 세포주인 SK-OV-3(ATCC, USA)을 1 ×104 세포수로 세포 배양액(GIBCOBRL, USA) 100 ㎕에 현탁한 후 96웰 플레이트의 각 웰에 접종하였다. 상기 실시예 3에서 제조한 송맥프라임을 40-50℃에서 24시간동안 열처리하여 건조 검체를 획득한 후 검체 검체 47 ㎎을 DMSO 200 ㎕에 용해시켰다. 100 ㎕ 배양액에 상기 제조된 송맥프라임 시료를 0, 0.6125, 1.25, 2.5, 5 또는 10 ㎍의 농도로 첨가하여 시료를 준비한 후 이것을 상기 세포가 접종된 각 웰에 첨가하여 2일동안 배양하였다. 배양 후 MTT 방법(Pieters et al., 1988, Cancer Letters. 41: 323-332)으로 세포독성 실험을 실시하였다.Human ovarian cancer cell line SK-OV-3 (ATCC, USA) was suspended in 100 μl of cell culture (GIBCOBRL, USA) in 1 × 10 4 cell numbers and then inoculated into each well of a 96 well plate. After obtaining the dry sample by heat-treating the trout prepared in Example 3 at 40-50 ℃ for 24 hours, 47 mg of the sample sample was dissolved in 200 μl DMSO. To prepare a sample by adding the prepared Songmak prime sample at a concentration of 0, 0.6125, 1.25, 2.5, 5 or 10 μg to 100 μl culture solution, it was added to each well inoculated with the cells and incubated for 2 days. After incubation, cytotoxicity experiments were performed by the MTT method (Pieters et al ., 1988, Cancer Letters . 41: 323-332).
그 결과, 송맥프라임을 첨가하지 않고 배양한 난소암 세포에 비해 송맥프라임을 첨가하여 배양한 결과, 첨가한 농도 의존적으로 세포 독성이 증가하여 생존률이 감소하였다(도 1).As a result, compared with the ovarian cancer cells cultured without the addition of trout, primed with the addition of trout, the concentration was dependent on the increased cytotoxicity was reduced survival rate (Fig. 1).
<5-2> 항암효과 2<5-2> Anticancer Effect 2
상기 실시예 3에서 제조한 솔잎 엑기스와 보리싹 엑기스의 혼합액을 DMSO에 녹였다. 상기 녹인 시료를 DMSO에 250, 500, 1000 또는 2500배 희석한 뒤 MEL-2 피부암 세포주(ATCC, USA)에 처리하야 상기 실시예 <5-1>과 동일한 방법으로 세포의 생존률을 측정하였다.The mixed solution of pine needle extract and barley sprout extract prepared in Example 3 was dissolved in DMSO. The dissolved samples were diluted 250, 500, 1000 or 2500 times in DMSO and then treated with MEL-2 skin cancer cell lines (ATCC, USA) to measure the survival rate of cells in the same manner as in Example <5-1>.
그 결과, 농도 의존적으로 세포의 생존률이 감소하였다(표 4).As a result, the survival rate of cells decreased in a concentration-dependent manner (Table 4).
<실시예 6> 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)의 항세균 효과 분석<Example 6> Antibacterial effect analysis of a mixture of pine needle extract and barley sprout extract (songmac prime)
상기 실시예 3에서 제조한 솔잎 엑기스와 보리싹 엑기스의 혼합액을 4000 rpm으로 20분 동안 원심분리한 뒤 상등액을 수득하였다. 상기 수득한 시료를 페이퍼 디스크(paper disk)에 각각 40 ㎕ 또는 120 ㎕로 적신 후 스타필로코커스 아우레우스(staphyllococcus aureus)를 도말한 영양배지아가(nutrient agar) 위에 올려놓아 37℃에서 밤새 배양하였다. 배양후 페이퍼 디스크 주위에 형성된 투명환(발육저지환)의 지름을 측정하였다.The supernatant was obtained after centrifuging the mixed solution of pine needle extract and barley sprout extract prepared in Example 3 at 4000 rpm for 20 minutes. The obtained sample was soaked in 40 μl or 120 μl of paper disk, respectively, and placed on a nutrient agar coated with staphyllococcus aureus, and incubated at 37 ° C. overnight. . After incubation, the diameter of the transparent ring (developing ring) formed around the paper disk was measured.
그 결과, 송맥프라임 40 ㎕로 적신 페이퍼 디스크에서는 스타필로코커스 아우레우스의 발육이 저지되지 않았지만 120 ㎕로 적신 디스크에서는 약 16 ㎜의 투명환이 형성되어 높은 항세균 효과가 있음을 알 수 있었다(도 2).As a result, the growth of Staphylococcus aureus was not inhibited in the paper disk moistened with 40 µl of trance, but it was found that about 16 mm of transparent rings were formed in the disk moistened with 120 µl of high antibacterial effect. 2).
<실시예 7> 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)의 항산화 효과 분석Example 7 Analysis of Antioxidant Effects of a Mixture of Pine Needle Extract and Barley Sprout Extract (Songmac Prime)
본 발명의 송맥프라임의 항산화 효과를 분석하기 위해 NBT 분석을 실시하였다. 구체적으로, Na2EDTA 버퍼(50mM KH2PO4/KOH, pH7.4) 20 ㎕, 6 mM NBT 50 ㎕, 3 mM 하이폭산틴 30 ㎕, 크산틴 옥시다제 50 ㎕, 송맥프라임 100 ㎕(건조검체 10 ㎍)를 혼합한 후 25℃에서 10분 동안 반응시켰다. 반응 후 반응액을 마이크로플래이트 분석기를 사용하여 570 nm에서 측정하였다. 양성 대조군(positive control)으로는 항산화제인 알로퓨리놀(Allopurinol)(Sigma)을 사용하였다. 그 결과를 하기 수학식에 의해 계산하여 항산화 효과를 분석하였다.NBT analysis was performed to analyze the antioxidant effect of the pine bark prime of the present invention. Specifically, 20 μl of Na 2 EDTA buffer (50 mM KH 2 PO 4 / KOH, pH7.4), 50 μl of 6 mM NBT, 30 μl of 3 mM hypoxanthine, 50 μl of xanthine oxidase, 100 μl of Songmaprime (dry) 10 μg of the sample was mixed and reacted at 25 ° C. for 10 minutes. After the reaction, the reaction solution was measured at 570 nm using a microplate analyzer. As a positive control, the antioxidant allopurinol (Sigma) was used. The results were calculated by the following equation to analyze the antioxidant effect.
<수학식>Equation
그 결과, 양성 대조군인 알로퓨리놀은 약 80%의 항산화 효과를 나타내었으며, 본 발명의 송맥프라임은 크루드(crude)한 상태로도 건조검체 10 ㎍/100㎕에서 82%의 항산화 효과를 나타내었다(표 5).As a result, allopurinol, a positive control, showed an antioxidant effect of about 80%, and the pine bark prime of the present invention exhibited an antioxidant effect of 82% in 10 μg / 100 μl of the dried sample even when it was crude ( Table 5).
<제조예 1> 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)을 유효성분으로 함유하는 건강 보조 식품의 제조Preparation Example 1 Preparation of a Health Supplement Containing a Mixture of Pine Needle Extract and Barley Sprout Extract (Song Mac Prime) as an Active Ingredient
<1-1> 송맥프라임을 함유하는 음료수의 제조<1-1> Preparation of Drinking Water Containing Songma Prime
본 발명자들은 상기 실시예 3에서 제조한 송맥프라임을 이용하여 건강 증진용 음료수를 하기 기재된 조성으로 통상적인 음료수 제조 방법을 사용하여 제조하였다.The inventors of the present invention was prepared using the conventional beverage preparation method for the health-enhanced beverage using the composition prepared in Example 3 in the composition described below.
꿀 522 ㎎522 mg of honey
치옥토산아미드 5 ㎎Chioctosanamide 5 mg
니코틴산아미드 10 ㎎Nicotinamide 10 mg
염산리보플라빈나트륨 3 ㎎Riboflavin Sodium Hydrochloride 3 mg
염산피리독신 2 ㎎Pyridoxine hydrochloride 2 mg
이노시톨 30 ㎎Inositol 30 mg
오르트산 50 ㎎Orthoic acid 50 mg
송맥프라임 0.5 ~ 1.5 ㎎Songmapri 0.5 ~ 1.5 ㎎
물 200 ㎖200 ml of water
<1-2> 송맥프라임을 함유하는 생식의 제조<1-2> Production of Raw Foods Containing Songma Prime
본 발명자들은 상기 실시예 3에서 제조한 송맥프라임을 이용하여 건강 증진용 생식을 하기 기재된 조성으로 통상적인 생식 제조 방법을 사용하여 제조하였다.The inventors of the present invention were prepared using the conventional reproductive manufacturing method in the composition described below for the health-promoting reproduction using the pineapple prime prepared in Example 3.
송맥프라임 분말 10%Songma Prime Powder 10%
현미 40%Brown rice 40%
율무 12%12%
수수 15%15% sorghum
검정콩 8%Black Bean 8%
케일 5%Kale 5%
들깨 10%Perilla 10%
<1-3> 송맥프라임을 함유하는 캔디의 제조<1-3> Preparation of Candy Containing Songma Prime
본 발명자들은 상기 실시예 3에서 제조한 송맥프라임을 이용하여 건강 증진용 생식을 하기에 기재된 조성으로 통상적인 캔디 제조 방법을 사용하여 제조하였다.The inventors of the present invention were prepared using the conventional candy manufacturing method with the composition described below for the health-promoting reproduction using the pineapple prime prepared in Example 3.
설탕 50 ∼ 60 %50 to 60% sugar
물엿 39.26 ∼ 49.66 %Starch syrup 39.26-49.66%
송맥프라임 0.24 ∼ 0.64 %Songmac Prime 0.24 ~ 0.64%
오렌지향 0.5 %Orange 0.5%
<1-4> 송맥프라임을 함유하는 츄잉껌의 제조<1-4> Preparation of Chewing Gum Containing Songma Prime
본 발명자들은 상기 실시예 3에서 제조한 송맥프라임을 이용하여 건강 증진용 생식을 하기 기재된 조성으로 통상적인 츄잉껌 제조 방법을 사용하여 제조하였다.The inventors of the present invention was prepared using the conventional chewing gum production method for the health-promoting reproduction using the composition prepared in Example 3 with the composition described below.
껌베이스 20 %Gum base 20%
설탕 76.36 ∼ 76.76 %Sugar 76.36-76.76%
송맥프라임 0.24 ∼ 0.64 %Songmac Prime 0.24 ~ 0.64%
후르츠향 1 %1% fruit flavor
물 2 %Water 2%
상기에서 살펴본 바와 같이, 본 발명에서 제조한 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)은 솔잎 및 보리싹의 주요 성분이 잘 보존되며, 항암, 항산화 및 항세균 효과가 뛰어나 건강 보조 식품으로 유용하게 사용될 수 있다.As described above, the mixture of pine needle extract and barley sprout extract (songmac prime) prepared in the present invention is well preserved the main components of pine needles and barley sprout, excellent anti-cancer, antioxidant and antibacterial effect is useful as a dietary supplement Can be used.
도 1은 본 발명의 방법에 의해 제조된 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)을 인간 난소암 세포주에 처리하여 배양하였을 때의 세포의 생존률을 나타낸 그래프이다.1 is a graph showing the survival rate of cells when the mixture of pine needle extract and barley sprout extract (songmac prime) prepared by the method of the present invention treated with human ovarian cancer cell line.
도 2는 본 발명의 방법에 의해 제조된 솔잎 엑기스와 보리싹 엑기스의 혼합물(송맥프라임)을 40 ㎕ 또는 120 ㎕로 적신 페이퍼 디스크를 이용하여 스타필로코커스 아우레우스에 대한 생육저지 효과를 본 그래프이다.Figure 2 is a graph showing the growth inhibitory effect on Staphylococcus aureus using a paper disk moistened with a mixture of pine needle extract and barley sprout extract (songmac prime) prepared by the method of the present invention in 40 μl or 120 μl. to be.
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