KR100386831B1 - a - Google Patents

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KR100386831B1
KR100386831B1 KR10-2000-0047544A KR20000047544A KR100386831B1 KR 100386831 B1 KR100386831 B1 KR 100386831B1 KR 20000047544 A KR20000047544 A KR 20000047544A KR 100386831 B1 KR100386831 B1 KR 100386831B1
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culture
incubator
present
chamber
supply
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KR10-2000-0047544A
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KR20020014328A (en
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김봉노
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주식회사 창진
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16—Solid state fermenters, e.g. for koji production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
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  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 연구소에서 각종 고체효소(균)를 배양하도록 하는 배양기에 관한 것으로The present invention relates to an incubator for culturing various solid enzymes (bacteria) in a laboratory

고체효소인 배양물질을 다양한 조건에서 배양과 실험 및 분석할 수 있도록 콤팩트형으로 설치되는 고체효소 배양기에 관한 것이다.The present invention relates to a solid enzyme incubator installed in a compact form so that the culture material, which is a solid enzyme, can be cultured and tested and analyzed under various conditions.

본 고안은 천정(6)에 설치된 각공(7)으로 열선(8)을 삽입하여 개폐되는 덮개(5)와, 상기 덮개(5)의 내부 배양실(2)에 배양판(11)을 설치하여 배양물질(12)을 교반하는 교반기(20)를 교반체인(25)으로 모터(21)에 연결하고 레일(19)에서 스프라켓(22)으로 왕복이동되는 교반장치(4)와, 상기 배양실(2)에 연결된 순환닥트 (18)를 히터(15)가 설치된 공조기(13)에 연결하고 급수탱크(16)로 부터 온수를 공급받아 온도와 습도를 조절하며 브로아(14)로 공급실(3)에 공급하는 배양기(1)로 이루어져 고체효소를 교반시키면서 설정된 온도와 습도로 균일하게 공급하여 소량을 실험, 분석할 수 있도록 하는 것이다.The present invention is incubated by installing a cover (5) to be opened and closed by inserting a heating wire (8) into the square hole (7) installed on the ceiling (6), and the culture plate (11) in the inner culture chamber (2) of the cover (5) An agitator 20 for agitating the substance 12 and a stirrer chain 25 connected to the motor 21 and reciprocating from the rail 19 to the sprocket 22 and the culture chamber 2 The circulation duct 18 connected to the air conditioner 13 installed with the heater 15 is supplied with hot water supplied from the water supply tank 16 to regulate temperature and humidity, and to supply the supply chamber 3 to the broa 14. It consists of the incubator (1) to uniformly supply the set temperature and humidity while stirring the solid enzyme to be able to experiment and analyze a small amount.

Description

고체효소 배양기{a}Solid enzyme incubator {a}

본 발명은 연구소에서 각종 고체효소(균)를 배양하도록 하는 배양기에 관한것으로The present invention relates to an incubator for culturing various solid enzymes (bacteria) in a laboratory

고체효소인 배양물질을 다양한 조건에서 배양과 실험 및 분석할 수 있도록 콤팩트형으로 설치되는 고체효소 배양기에 관한 것이다.The present invention relates to a solid enzyme incubator installed in a compact form so that the culture material, which is a solid enzyme, can be cultured and tested and analyzed under various conditions.

일반적으로 연구소나 각종 실험실에서는 일정한 조건하에서 고체효소를 배양하는 과정을 갖게 된다.In general, laboratories and various laboratories have a process of culturing solid enzymes under certain conditions.

그러나 종래에는 고체효소를 배양하는 과정이 인큐베이터와 같은 내부에 고체효소를 넣고 온도를 설정하여 가열하는 방식이므로 온도를 내려주는 기능은 없으며, 온도를 올려주는 기능만 있는 단순한 것이었다.However, conventionally, the process of culturing the solid enzyme is a method of putting the solid enzyme inside the incubator, such as by setting the temperature and heating the temperature, so there is no function of lowering the temperature, but only a function of raising the temperature.

또한 온도를 자유롭게 조절할 수 없을 뿐만아니라 가습을 할 수 없어서 고체효소를 용이하게 배양할 수 없어 실험, 분석이 어렵고, 정확한 분석자료를 제공할 수 없었다.In addition, the temperature can not be freely controlled, and because it is not humidified, solid enzymes cannot be easily cultured, making it difficult to experiment and analyze, and provide accurate analytical data.

본 발명은 온도와 가습, 산소공급 및 CO2의 배출을 자동으로 조절할 수 있도록 하여 소량의 효소를 배양과 실험 및 분석할 수 있도록 한다.The present invention enables to automatically control the temperature and humidification, oxygen supply and CO 2 emissions to be able to culture, experiment and analyze a small amount of enzyme.

본 발명은 배양실의 내부에 공급된 고체효소를 교반시켜 균일한 배양을 가능하게 한다.The present invention enables uniform culture by stirring the solid enzyme supplied into the culture chamber.

본 발명은 콤팩트형으로 운반 및 이동이 간편하며, 천정에서 결로현상을 방지하도록 하는 것이다.The present invention is compact and easy to transport and move, to prevent condensation on the ceiling.

본 발명은 천정에 설치된 각공으로 열선을 삽입하여 개폐되는 덮개와, 상기 덮개의 내부 배양실에 배양판을 설치하여 배양물질을 교반하는 교반기를 교반체인으로 모터에 연결하고 레일에서 스프라켓으로 왕복이동되는 교반장치와,The present invention is a cover that is opened and closed by inserting a hot wire into the square hole installed in the ceiling, and the culture plate is installed in the inner culture chamber of the cover, the agitator for agitating the culture material is connected to the motor with a stirring chain and agitated reciprocating from the rail to the sprocket Device,

상기 배양실에 연결된 순환닥트를 히터가 설치된 공조기에 연결하고 급수탱크로 부터 온수를 공급받아 온도와 습도를 조절하며 브로아로 공급실에 공급하는 배양기로 이루어진 것을 특징으로 하는 것이다.The circulating duct connected to the culture chamber is connected to an air conditioner installed with a heater, and receives hot water from a water supply tank to adjust temperature and humidity, and characterized in that the incubator is supplied to the supply chamber as broa.

도 1 은 본 발명의 내부를 보인 설치상태도1 is an installation state showing the inside of the present invention

도 2 는 본 발명의 내부를 보인 평면도Figure 2 is a plan view showing the interior of the present invention

도 3 은 본 발명의 배양판에 대한 평면도3 is a plan view of the culture plate of the present invention

도 4 는 본 발명의 배양판에 대한 단면도Figure 4 is a cross-sectional view of the culture plate of the present invention

[도면의 주요 부분에 대한 부호의 설명][Description of Symbols for Main Parts of Drawing]

1 : 배양기 2 : 배양실1: incubator 2: incubation chamber

3 : 공급실 4 : 교반장치3: supply chamber 4: stirring device

5 : 덮개 6 : 천정5: cover 6: ceiling

7 : 각공 8 : 열선7: square hole 8: heat wire

10 : 패킹 11 : 배양판10 packing 11: culture plate

11' : 타공 12 : 배양물질11 ': perforation 12: culture material

13 : 공조기 20 : 교반기13: air conditioner 20: agitator

본 발명의 배양기(1)는 실내에 배양실(2)과 공급실(3)이 설치되며, 외부에는 공조기(13)를 이용하여 내부의 온도와 습도를 조절한다.In the incubator 1 of the present invention, the culture chamber 2 and the supply chamber 3 are installed indoors, and the outside temperature and humidity are controlled by using an air conditioner 13.

상기 배양기(1)는 배양실(2)의 상측으로 천정(6)을 이루는 덮개(5)가 사면에서 패킹(10)으로 밀폐되고 경첩(9)이 설치되어 개폐를 가능하게 한다.The incubator 1 has a lid 5 forming the ceiling 6 to the upper side of the culture chamber 2 is sealed by the packing 10 on the slope and the hinge 9 is installed to enable the opening and closing.

덮개(5)의 하측인 천정(6)에는 일정한 간격으로 각공(7)이 설치되어 열선(8)을 삽입하여 배양실(2)의 온도와 습도로 인하여 천정(6)에서 발생되는 결로현상을 방지하는 것이다.Square holes 6, which are lower side of the cover 5, are provided with square holes 7 at regular intervals to prevent condensation from occurring on the ceiling 6 due to the temperature and humidity of the culture chamber 2 by inserting the hot wire 8. It is.

즉, 열선(8)이 발열되어 천정(6)에서 발생되는 결로현상을 방지하고, 열선 (8)이 발열되지 않으면 각공(7)에서 간편하게 분리하여 새로운 열선으로 교체할 수 있는 것이다.That is, the heating wire 8 generates heat to prevent condensation from occurring in the ceiling 6, and if the heating wire 8 does not heat, it can be easily separated from each hole 7 and replaced with a new heating wire.

배양실(2)의 내부 바닥에는 타공(11')이 형성된 배양판(11)이 설치되고 양측으로 레일(19)이 설치되어 교반장치(4)를 좌,우측으로 이동되도록 한다.A culture plate 11 having a perforation 11 ′ is installed at an inner bottom of the culture chamber 2, and rails 19 are installed at both sides to move the stirring device 4 left and right.

교반장치(4)는 배양판(11)과 근접하도록 교반기(20)가 설치되어 교반체인 (25)으로 모터(21)와 연결되고, 레일(19)에 올려져 있는 스프라켓(22)과 이송체인 (24)으로 연결되어 모터(21)의 동력으로 교반장치(4)를 좌,우측으로 수평이동시키면서 교반기(20)를 회전시켜 배양판(11)상에 공급된 배양물질(12)을 교반할 수 있도록 하는 것이다.The stirring device 4 is provided with a stirrer 20 so as to be close to the culture plate 11, connected to the motor 21 by a stirring chain 25, and a sprocket 22 and a transfer chain mounted on the rail 19. Is connected to the 24 to rotate the stirrer 20 while moving the stirring device 4 to the left and right horizontally by the power of the motor 21 to stir the culture material 12 supplied on the culture plate 11 To make it possible.

배양실(2)의 하측에는 공급실(3)이 형성되어 부로아(14)를 통하여 공급되는 산소가 타공(11')을 통하여 배양물질(12)에 공급되도록 한다.A supply chamber 3 is formed below the culture chamber 2 so that oxygen supplied through the broro 14 is supplied to the culture material 12 through the perforations 11 '.

상기 배양실(2)의 일측에는 순환닥트(18)가 설치되어 배양실(2)에서 공기를 배기시키며, 공기중의 CO2를 배출시키게 된다.A circulating duct 18 is installed at one side of the culture chamber 2 to exhaust air from the culture chamber 2 and to discharge CO 2 in the air.

이중 일부는 외부로 배출시키고, 일부는 공조기(13)에 공급하여 순환되도록 한다.Some of them are discharged to the outside, and some are supplied to the air conditioner 13 to be circulated.

공조기(13)는 브로아(14)를 통하여 공급실(3)에 연결되며, 급수탱크(16)의 히팅코일(17)을 통하여 히팅된 온수를 공급받도록 하고, 일측으로 히터(15)를 설치하여 습도와 온도를 조절할 수 있도록 하는 것이다.The air conditioner 13 is connected to the supply chamber 3 through the broa 14, to receive the heated hot water through the heating coil 17 of the water supply tank 16, and to install the heater 15 to one side to humidity And to control the temperature.

본 발명은 배양기(1)의 덮개(5)를 열고 배양판(11)의 상측으로 실험하기 위한 배양물질(12)을 공급한다.The present invention opens the cover 5 of the incubator 1, and supplies the culture material 12 for experimenting to the upper side of the culture plate (11).

배양물질(12)을 공급한 후 덮개(5)를 닫아 배양실(2)을 밀폐시킨 후 실험 및 분석하기 위한 온도, 습도를 세팅한다.After supplying the culture material 12, the cover 5 is closed to seal the culture chamber 2, and then the temperature and humidity for experiment and analysis are set.

그러면 급수탱크(16)의 히팅코일(17)에 의하여 히팅된 온수는 공조기(13)에 공급되어 분사되는 동시에 히터(15)에서 더운 바람이 발생되어 설정된 온도, 습도를 유지하면서 공급된다.Then, the hot water heated by the heating coil 17 of the water supply tank 16 is supplied to the air conditioner 13 and sprayed, and at the same time, hot wind is generated from the heater 15 to maintain the set temperature and humidity.

설정된 온도, 습도를 유지하면서 공급되면 브로아(14)를 통하여 공급실(3)에 공급되어 배양판(11)의 타공(11')으로 설정된 온도, 습도가 공급되어서 배양물질(12)과 접촉된다.When supplied while maintaining the set temperature, humidity is supplied to the supply chamber (3) through the broa 14, the temperature and humidity set to the perforations (11 ') of the culture plate 11 is supplied to contact the culture material (12).

이때 교반장치(4)의 모터(21)를 구동시키면 이송체인(24)으로 스프라켓(22)을 회전시켜 레일(19)상에서 좌,우측으로 왕복이동 된다.At this time, when the motor 21 of the stirring device 4 is driven, the sprocket 22 is rotated by the transfer chain 24 to reciprocate left and right on the rail 19.

레일(19)의 양측에는 통상적인 리미트스위치를 설치하여 교반장치(4)가 왕복이동되는 거리를 설정할 수 있으며, 교반체인(25)으로 교반기(20)를 회전시켜 배양물질(12)을 일정한 시간 간격으로 교반시켜 골고루 배양이 이루어지도록 한다.On both sides of the rail 19, a conventional limit switch can be installed to set the distance at which the stirrer 4 is reciprocated. The stirrer 20 is rotated by the stirring chain 25 to rotate the culture material 12 for a predetermined time. Stir at intervals to ensure even incubation.

상기 배양실(2)의 온도, 습도를 자동으로 조절할 수 있으며, 산소공급 및 CO2의 배출을 자동으로 조절할 수 있는 것이다.The temperature and humidity of the culture chamber (2) can be automatically adjusted, and the oxygen supply and CO 2 can be automatically adjusted.

본 발명은 입력에 의해서 온도를 자동으로 콘트롤할 수 있으며, 그 기록이 남아있도록 하여 연구과정을 확인할 수 있도록 하는 것이다.The present invention is able to automatically control the temperature by the input, so that the record remains so that the research process can be confirmed.

본 발명은 콤팩트하게 성형된 배양기를 이용하여 연구소에서 고체효소를 공급하여 온도, 습도, 산소의 공급과, CO2의 배출을 자동으로 조절할 수 있으므로 소량의 효소를 분석, 실험할 수 있는 장비를 제공한다.The present invention provides a device capable of analyzing and experimenting a small amount of enzymes by supplying a solid enzyme in a laboratory using a compactly-formed incubator to automatically adjust temperature, humidity, oxygen supply, and CO 2 emission. do.

본 발명은 바이오 제품의 개발을 위해서 필수적으로 필요한 장비이며, 이동이 편리하고 좁은공간(실험실, 연구실)의 실험, 분석이 용이한 것이다.The present invention is a necessary equipment for the development of a bio-product, it is easy to move and easy to experiment and analyze in a narrow space (laboratory, laboratory).

본 발명은 뚜껑의 개폐를 가능하게 하고, 교반장치를 왕복이동시켜 배양물질을 교반시키게 되므로 배양판의 타공을 통하여 배양물질에 균일한 온도와 습도가 유지되도록 한다.The present invention enables the opening and closing of the lid, and by stirring the culture material by reciprocating the stirring device to maintain a uniform temperature and humidity in the culture material through the perforation of the culture plate.

본 발명은 이동 및 설치가 간편하고 자동제어를 가능하게 하며, 천정에 설치된 열선을 통하여 결로현상을 방지하는 것이다.The present invention is easy to move and install, to enable automatic control, and to prevent condensation through a heating wire installed on the ceiling.

Claims (1)

천정(6)에 설치된 각공(7)으로 열선(8)을 삽입하여 개폐되는 덮개(5)와,A cover 5 which is opened and closed by inserting the heating wire 8 into the square hole 7 installed in the ceiling 6, 상기 덮개(5)의 내부 배양실(2)에 배양판(11)을 설치하여 배양물질(12)을 교반하는 교반기(20)를 교반체인(25)으로 모터(21)에 연결하고 레일(19)에서 스프라켓(22)으로 왕복이동되는 교반장치(4)와,The culture plate 11 is installed in the inner culture chamber 2 of the cover 5, and the stirrer 20 for stirring the culture material 12 is connected to the motor 21 with the stirring chain 25 and the rail 19. A stirring device (4) to reciprocate to the sprocket (22), 상기 배양실(2)에 연결된 순환닥트(18)를 히터(15)가 설치된 공조기(13)에 연결하고 급수탱크(16)로 부터 온수를 공급받아 온도와 습도를 조절하며 브로아 (14)로 공급실(3)에 공급하는 배양기(1)로 이루어진 것을 특징으로 하는 고체효소 배양기.The circulation duct 18 connected to the culture chamber 2 is connected to the air conditioner 13 in which the heater 15 is installed, receives hot water from the water supply tank 16, adjusts temperature and humidity, and supplies the supply chamber to the broa 14 ( Solid enzyme incubator, characterized in that consisting of the incubator (1) to supply to 3).
KR10-2000-0047544A 2000-08-17 2000-08-17 a KR100386831B1 (en)

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KR20030082231A (en) * 2002-04-17 2003-10-22 주식회사 창진 a
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59198967A (en) * 1983-04-26 1984-11-10 Nagata Jozo Kikai Kk Preparation of koji
KR860000495U (en) * 1984-01-15 1986-02-13 김봉노 Imperial stirrer
US5521092A (en) * 1993-02-25 1996-05-28 Frank Alex Erich Rindelaub Fermentation plant
JPH10182270A (en) * 1996-12-26 1998-07-07 Chiyoda Tech & Ind Co Ltd Agitator for circular fermenter
JPH11300328A (en) * 1998-04-22 1999-11-02 Mung-Supu Lee Garbage low temperature natural fermentation drying method and device therefor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59198967A (en) * 1983-04-26 1984-11-10 Nagata Jozo Kikai Kk Preparation of koji
KR860000495U (en) * 1984-01-15 1986-02-13 김봉노 Imperial stirrer
US5521092A (en) * 1993-02-25 1996-05-28 Frank Alex Erich Rindelaub Fermentation plant
JPH10182270A (en) * 1996-12-26 1998-07-07 Chiyoda Tech & Ind Co Ltd Agitator for circular fermenter
JPH11300328A (en) * 1998-04-22 1999-11-02 Mung-Supu Lee Garbage low temperature natural fermentation drying method and device therefor

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