CN217466943U

CN217466943U - Antibody incubation device

Info

Publication number: CN217466943U
Application number: CN202221104733.6U
Authority: CN
Inventors: 冷毅斌; 杨飞; 华浩然
Original assignee: Yilairuite Wuhan Biotechnology Co ltd
Current assignee: Yilairuite Wuhan Biotechnology Co ltd
Priority date: 2022-05-07
Filing date: 2022-05-07
Publication date: 2022-09-20
Anticipated expiration: 2032-05-07

Abstract

The utility model discloses a device is hatched to antibody. The device comprises an incubation box and a temperature control box, wherein the incubation box is detachably arranged in the temperature control box, a test component comprising a visible light simulation lamp, a temperature detector, a shaking component and a temperature control component is arranged in the temperature control box, and the visible light simulation lamp simulates sunlight to irradiate so as to meet the incubation of antibodies with different requirements; shaking the assembly to achieve sufficient contact of the contents of the incubation well; the temperature control assembly is used for regulating and controlling the temperature inside the incubation box, the cooling effect is good, and the incubation efficiency of the Western blot antibody is improved; the temperature detector is arranged to realize real-time monitoring of the temperature inside the incubation box, and test failure caused by incubation of the antibody due to unstable temperature is prevented. And the utility model discloses the device is simple to use, and convenient operation, the effectual antibody of having guaranteed is hatched efficiency, has saved antibody reagent, has improved staff's work efficiency, has reduced experimental error, reduces experimental failure rate, has saved antibody reagent.

Description

Antibody incubation device

Technical Field

The utility model relates to a western blotting technical field especially relates to a device is hatched to antibody.

Background

Western blotting, also known as Western blotting, is an analytical technique for separating proteins by gel electrophoresis and then reacting with specific antibodies to detect specific proteins in a sample. In the Western Blot experiment, some experiments need to be incubated with antibodies in a low-temperature environment, some experiments need to be incubated with antibodies in a normal-temperature environment, and the incubation box containing the antibodies needs to be placed on an incubation box shaking table to shake on the premise of ensuring proper temperature, so that the antibody diluent can fully contact the PVDF membrane. The whole Western Blot experiment is completed, the process is complex, a plurality of test devices are needed, and inconvenience is brought to the operation of workers.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an antibody incubation device to the above-mentioned not enough of prior art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides an antibody incubation device, which comprises an incubation box and an incubation box; the incubator comprises a box body, a visible light simulation lamp, a shaking assembly and a temperature control assembly, wherein the front side of the box body is open, a cabinet door for opening or closing the incubator door is arranged at the opening, and an air outlet is formed in the bottom of the box body; visible light simulation lamp can dismantle the setting and be in incubator inner wall top, the accuse temperature subassembly sets up in the box for its inside temperature of control adjustment, the subassembly setting of shaking is in the box, it installs to hatch box detachable shake on the subassembly.

Furthermore, the temperature control assembly comprises a temperature detector, a semiconductor refrigerating piece, a semiconductor heating piece and a controller, the temperature detector, the semiconductor refrigerating piece and the semiconductor heating piece are arranged in the box body, and the temperature detector, the semiconductor refrigerating piece and the semiconductor heating piece are respectively electrically connected with the controller.

Further, the shaking assembly comprises a vibrator, a tray and at least one clamping mechanism, the vibrator is arranged at the bottom of the box body, the tray is detachably arranged on the vibrator, the at least one clamping mechanism is arranged on the tray, and the at least one clamping mechanism is used for fixing and clamping the incubation box.

Further, the visible light simulation lamp is a deuterium lamp.

Furthermore, a placing plate is detachably arranged in the incubator.

Further, it is in to shelve the board and can dismantle the setting through installation component on the incubator inner wall, installation component includes sliding tray and sliding strip, the sliding tray sets up on the both sides inner wall of incubator, shelve the both ends of board and install the sliding strip, sliding strip slidable sets up in the sliding tray.

Furthermore, the placing plate is provided with a plurality of groups, and the placing plate is provided with ventilation holes.

Furthermore, the outer edge of the joint of the cabinet door and the box body is wrapped with a sealing rubber sleeve, and the cabinet door is fixed with a handrail.

Further, it includes box body and the lid of sealed on box body to incubate the box, longitudinal parallel interval is provided with a plurality of baffle in the box body, and a plurality of baffle will the box body is cut apart into a plurality of independent incubation chamber.

The utility model provides a beneficial effect that technical scheme brought is:

(1) the utility model discloses an antibody incubation device, the incubation box can be disassembled and arranged in the incubation box, the box body, the visible light analog lamp, the shaking component and the temperature control component are arranged in the incubation box, the visible light analog lamp simulates sunlight to irradiate, and the antibody incubation meeting different requirements is realized; shaking the assembly to achieve sufficient contact of the contents of the incubation well; the temperature control assembly is used for regulating and controlling the temperature inside the incubation box, the cooling effect is good, and the incubation efficiency of the Western blot antibody is improved; the box provides sealed space, prevents to lead to the fact experimental failure because of the unstable influence antibody of temperature is hatched.

(2) The utility model discloses a device is hatched to antibody, simple to use, convenient operation, the effectual antibody of having guaranteed is hatched efficiency, has improved staff's work efficiency, has reduced experimental error, reduces experimental failure rate, has saved antibody reagent.

Drawings

FIG. 1 is a schematic structural view of an antibody incubation device according to the present invention;

fig. 2 is a schematic structural view of the incubation box of the antibody incubation device of the present invention.

1. An incubation box; 11. a box body; 12. a box cover; 13. a partition plate; 14. an incubation cavity; 2. an incubator; 21. a box body; 211. a cabinet door; 212. a sealing strip; 213. a handrail; 22. a visible light analog lamp; 23. a shaking assembly; 231. a vibrator; 232. a tray; 233. a clamping mechanism; 24. a temperature control assembly; 241. a temperature detector; 242. a semiconductor refrigerating sheet; 243. a semiconductor heat generating sheet; 244. a controller; 25. a shelf board; 251. mounting the component; 252. a sliding groove; 253. a slide bar.

Detailed Description

The following are specific embodiments of the present invention and the accompanying drawings are used to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

As shown in fig. 1, the antibody incubation device of the present invention comprises an incubation box 1 and an incubation box 2; the incubator 2 comprises a box body 21, a visible light simulation lamp 22, a shaking assembly 23 and a temperature control assembly 24, wherein the front side of the box body 21 is open, a cabinet door 211 for opening or closing the opening is arranged at the opening, and an air outlet is arranged at the bottom of the box body 21; visible light simulation lamp 22 can be dismantled and set up at incubator 2 inner wall top, and accuse temperature subassembly 24 sets up in box 21 for its inside temperature of control adjustment, shakes subassembly 23 and sets up in the box, and incubator 1 detachable installs on shaking subassembly 23. The incubation box is detachably arranged in the incubation box, the box body, the visible light simulation lamp, the shaking assembly and the temperature control assembly are arranged in the incubation box, the visible light simulation lamp simulates sunlight to irradiate, and the incubation of the antibodies meeting different requirements is realized; shaking the assembly to achieve sufficient contact of the contents of the incubation well; the temperature control assembly is used for regulating and controlling the temperature inside the incubation box, the cooling effect is good, and the incubation efficiency of the Western blot antibody is improved; the box provides sealed space, prevents to lead to the fact experimental failure because of the unstable influence antibody of temperature is hatched. And the utility model discloses the device is simple to use, and convenient operation, the effectual antibody of having guaranteed is hatched efficiency, has saved antibody reagent, has improved staff's work efficiency, has reduced experimental error, reduces experimental failure rate, has saved antibody reagent.

In order to satisfy the antibody incubation test that different temperatures required, improve the antibody incubation efficiency, the utility model discloses an antibody incubation device can set up accuse temperature subassembly 24. The temperature control assembly 24 can be constructed in a variety of ways and is not limited thereto. In this embodiment, the temperature control assembly 24 includes a temperature detector 241, a semiconductor cooling plate 242, a semiconductor heating plate 243 and a controller 244, the temperature detector 241, the semiconductor cooling plate 242 and the semiconductor heating plate 243 are disposed in the box body 21, and the temperature detector 241, the semiconductor cooling plate 242 and the semiconductor heating plate 243 are respectively electrically connected to the controller 245. This accuse temperature subassembly 24 can provide stable temperature for incubator 2, satisfies different temperature incubation conditions, is favorable to the staff operation, has reduced experimental error simultaneously effectively.

In order to guarantee that the contact of the content in the incubation box 1 is sufficient, the utility model discloses an antibody incubation device can set up the subassembly 23 that shakes. The structure of the shaking assembly 23 is various and is not limited herein. In this embodiment, the shaking assembly 23 may include a vibrator 231, a tray 232, and at least one clamping mechanism 233, the vibrator 231 is disposed at the bottom of the case 21, the tray 232 is detachably disposed on the vibrator 231, the at least one clamping mechanism 233 is disposed on the tray 232, and the clamping mechanism 233 is used to fix and clamp the incubation cartridge 1. In use, the plurality of incubation cartridges 1 containing the antibody are fixed to the tray 232 by the holding mechanism 233, and the vibrator 231 is started to start shaking the incubation cartridges 1. The antibody incubation efficiency is improved and experimental failure is avoided.

In order to meet the requirements of antibodies with different performances, a visible light simulation lamp 22 is arranged on the top of the inner wall of the incubator 2, and the visible light simulation lamp 22 can be a deuterium lamp. Open visible light simulation lamp 22 and be favorable to needing one anti hatching of visible light irradiation, close visible light simulation lamp 22, the cabinet door 211 of box 21 closes, the inside darkroom that is of box 21, be favorable to needing two anti hatching of light-resistant, can satisfy one anti demand that needs irradiant, also can satisfy two anti demands of light-resistant, can carry out the hatching of two kinds of samples simultaneously and detect, antibody solution has been practiced thrift, 1 washing times of hatching box has still been reduced, bring the facility for the staff, and the work efficiency is improved.

For the utility model discloses a device application scope is wide for the antibody, cultivates at the constant temperature of various liquid, solid-state samples such as biochemistry, cell, bacterial, can also set up in the inside of incubator 2 and put board 25.

For convenience of use and clean maintenance, the resting plate 25 may be detachably disposed on the inner wall of the incubator 2 through a mounting assembly 251, the mounting assembly 251 includes a sliding groove 252 and a sliding bar 253, the sliding groove 252 is disposed on the inner walls of both sides of the incubator 2, the sliding bar 253 is mounted at both ends of the resting plate 25, and the sliding bar 253 is slidably disposed in the sliding groove.

In order to ensure that the temperature in the box body 21 is constant and more samples can be incubated, the placing plate 25 can have multiple groups, and the placing plate 25 is provided with vent holes to ensure that air in the box body 21 is uniform in cooling and heating.

In order to provide a better sealing effect for the box body 21 and ensure the temperature stability in the box body 21, the outer edge of the joint of the cabinet door 211 and the box body 21 is wrapped with a sealing strip 212.

In order to facilitate the operation of the worker, an armrest 213 is fixed on the cabinet door 211.

As shown in fig. 2, in order to solve the problems of serious antibody waste and inflexible size use of the antibody incubation box in the western blotting experiment, in the present embodiment, the incubation box 1 may include a box body 11 and a box cover 12 sealed on the box body, a plurality of partition plates 13 are longitudinally arranged in parallel in the box body 11 at intervals, and the box body 11 is divided into a plurality of independent incubation cavities 14 by the plurality of partition plates. The sealed environment reduces evaporation of the antibody solution.

The above is not relevant and is applicable to the prior art.

While certain specific embodiments of the present invention have been described in detail by way of illustration, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and not limitation, as various modifications or additions may be made to the described specific embodiments or alternatives thereto, as will be apparent to those skilled in the art, without departing from the scope of the invention or exceeding the scope of the claims. It should be understood by those skilled in the art that any modifications, equivalent substitutions, improvements and the like made to the above embodiments according to the technical spirit of the present invention should be included in the scope of the present invention.

Claims

1. An antibody incubation device, comprising: comprises an incubation box (1) and an incubation box (2); the incubator (2) comprises a box body (21), a visible light simulation lamp (22), a shaking component (23) and a temperature control component (24), wherein the front side of the box body (21) is open, a cabinet door (211) for opening or closing the box body is arranged at the opening, and an air outlet is formed in the bottom of the box body (21); visible light simulation lamp (22) can be dismantled the setting and be in incubator (2) inner wall top, accuse temperature subassembly (24) set up in box (21) for its inside temperature of control adjustment, shake subassembly (23) and set up in the box, it installs to hatch box (1) detachable shake on subassembly (23).

2. An antibody incubation device according to claim 1, wherein: the temperature control assembly (24) comprises a temperature detector (241), a semiconductor refrigerating sheet (242), a semiconductor heating sheet (243) and a controller (244), wherein the temperature detector (241), the semiconductor refrigerating sheet (242) and the semiconductor heating sheet (243) are arranged in the box body (21), and the temperature detector (241), the semiconductor refrigerating sheet (242) and the semiconductor heating sheet (243) are respectively electrically connected with the controller (244).

3. Antibody incubation device according to claim 1, wherein the shaking assembly (23) comprises a vibrator (231), a tray (232) and at least one clamping mechanism (233), the vibrator (231) being arranged at the bottom of the housing (21), the tray (232) being detachably arranged on the vibrator (231), the clamping mechanism (233) being arranged on the tray (232) for fixing and clamping the incubation cartridge (1).

4. The antibody incubation device of claim 1, wherein the visible light simulated lamp (22) is a deuterium lamp.

5. Antibody incubation device according to claim 1, characterized in that a resting plate (25) is removably arranged inside the incubator (2).

6. Antibody incubation device according to claim 5, wherein the resting plate (25) is removably arranged on the inner wall of the incubator (2) by means of a mounting assembly (251), the mounting assembly (251) comprising a sliding groove (252) and a sliding bar (253), the sliding groove (252) being arranged on the inner wall of both sides of the incubator (2), the sliding bar (253) being mounted at both ends of the resting plate (25), the sliding bar (253) being slidably arranged in the sliding groove.

7. Antibody incubation device according to claim 5, characterized in that said resting plate (25) is provided in a plurality of groups, said resting plate (25) being provided with ventilation holes.

8. Antibody incubation device according to claim 1, characterized in that: the outer edge of the joint of the cabinet door (211) and the box body (21) is coated with a sealing strip (212), and a handrail (213) is fixed on the cabinet door (211).

9. The antibody incubation device according to claim 1, wherein the incubation box (1) comprises a box body (11) and a box cover (12) sealed on the box body, a plurality of partition plates (13) are longitudinally arranged in the box body (11) in parallel at intervals, and the plurality of partition plates divide the box body (11) into a plurality of independent incubation cavities (14).