JPWO2020097414A5 - - Google Patents
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- JPWO2020097414A5 JPWO2020097414A5 JP2021525240A JP2021525240A JPWO2020097414A5 JP WO2020097414 A5 JPWO2020097414 A5 JP WO2020097414A5 JP 2021525240 A JP2021525240 A JP 2021525240A JP 2021525240 A JP2021525240 A JP 2021525240A JP WO2020097414 A5 JPWO2020097414 A5 JP WO2020097414A5
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- Prior art keywords
- nucleotides
- polynucleotide
- seq
- optionally
- mrna
- Prior art date
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- 239000002773 nucleotide Substances 0.000 claims description 78
- 125000003729 nucleotide group Chemical group 0.000 claims description 78
- 108020004999 Messenger RNA Proteins 0.000 claims description 19
- 229920002106 messenger RNA Polymers 0.000 claims description 19
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 11
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims description 10
- 230000000295 complement Effects 0.000 claims description 5
- 229920000023 polynucleotide Polymers 0.000 claims 24
- 239000002157 polynucleotide Substances 0.000 claims 24
- 241000238631 Hexapoda Species 0.000 claims 15
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 13
- 108020004461 Double-Stranded RNA Proteins 0.000 claims 7
- 241000254173 Coleoptera Species 0.000 claims 6
- 241000196324 Embryophyta Species 0.000 claims 6
- 229920002477 rna polymer Polymers 0.000 claims 6
- 239000012141 concentrate Substances 0.000 claims 4
- 108020004394 Complementary RNA Proteins 0.000 claims 2
- 241000258915 Leptinotarsa Species 0.000 claims 2
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 claims 2
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 claims 2
- 239000003184 complementary RNA Substances 0.000 claims 2
- 230000037213 diet Effects 0.000 claims 2
- 235000005911 diet Nutrition 0.000 claims 2
- 239000000839 emulsion Substances 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 241000902876 Alticini Species 0.000 claims 1
- 241000219317 Amaranthaceae Species 0.000 claims 1
- 241000254177 Anthonomus Species 0.000 claims 1
- 241000208173 Apiaceae Species 0.000 claims 1
- 241000219193 Brassicaceae Species 0.000 claims 1
- 241001124145 Cerotoma Species 0.000 claims 1
- 241000219104 Cucurbitaceae Species 0.000 claims 1
- 241000489975 Diabrotica Species 0.000 claims 1
- 241000258916 Leptinotarsa decemlineata Species 0.000 claims 1
- 241000219071 Malvaceae Species 0.000 claims 1
- 241000275067 Phyllotreta Species 0.000 claims 1
- 241000209504 Poaceae Species 0.000 claims 1
- 241000208292 Solanaceae Species 0.000 claims 1
- 241000254086 Tribolium <beetle> Species 0.000 claims 1
- 239000000654 additive Substances 0.000 claims 1
- 230000000996 additive Effects 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 230000000240 adjuvant Effects 0.000 claims 1
- 239000000443 aerosol Substances 0.000 claims 1
- 239000005667 attractant Substances 0.000 claims 1
- -1 biologics Substances 0.000 claims 1
- 229960000074 biopharmaceuticals Drugs 0.000 claims 1
- 239000002775 capsule Substances 0.000 claims 1
- 239000007963 capsule composition Substances 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 235000014633 carbohydrates Nutrition 0.000 claims 1
- 230000031902 chemoattractant activity Effects 0.000 claims 1
- 230000035613 defoliation Effects 0.000 claims 1
- 239000000428 dust Substances 0.000 claims 1
- 239000004495 emulsifiable concentrate Substances 0.000 claims 1
- 239000003897 fog Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 239000002316 fumigant Substances 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 239000003630 growth substance Substances 0.000 claims 1
- 239000008241 heterogeneous mixture Substances 0.000 claims 1
- 239000008240 homogeneous mixture Substances 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 230000002262 irrigation Effects 0.000 claims 1
- 238000003973 irrigation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000003094 microcapsule Substances 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 150000002894 organic compounds Chemical class 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 239000000575 pesticide Substances 0.000 claims 1
- 239000003016 pheromone Substances 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000000779 smoke Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 102100010833 PSMB5 Human genes 0.000 description 9
- 101710033548 PSMB5 Proteins 0.000 description 9
- 230000025458 RNA interference Effects 0.000 description 8
- 229920002676 Complementary DNA Polymers 0.000 description 2
- 231100000822 oral exposure Toxicity 0.000 description 2
Description
いくつかの態様において、PSMB5を標的とするRNAi分子は、Coleopteran PSMB5 DNAの領域またはセグメントによってコードされるmRNAに相補的な配列を含むか、またはそれらからなる。いくつかの態様において、RNAi分子は、PSMB5 DNA(例として、配列番号1のヌクレオチド1~450)のヌクレオチド1~500、ヌクレオチド10~500、ヌクレオチド25~500、ヌクレオチド50~500、ヌクレオチド100~500、ヌクレオチド150~500、ヌクレオチド200~500、ヌクレオチド250~500、ヌクレオチド300~500、ヌクレオチド350~500、ヌクレオチド400~500、またはヌクレオチド450~500に包含される任意の配列を含むか、またはそれらからなってもよい、Coleopteran PSMB5 DNAの領域によってコードされるmRNAを標的とする。いくつかの態様において、RNAi分子は、PSMB5 DNA(例として、配列番号1のヌクレオチド450~927)のヌクレオチド200~950、ヌクレオチド250~950、ヌクレオチド300~950、ヌクレオチド350~950、ヌクレオチド400~950、ヌクレオチド450~950、ヌクレオチド500~950、ヌクレオチド550~950、ヌクレオチド200~700、ヌクレオチド250~700、ヌクレオチド300~700、ヌクレオチド350~700、ヌクレオチド400~700、ヌクレオチド450~700、ヌクレオチド500~700、ヌクレオチド550~700、ヌクレオチド600~700、またはヌクレオチド650~700に包含される任意の配列を含むか、またはそれらからなってもよい、Coleopteran PSMB5 DNAの領域によってコードされるmRNAを標的とする。いくつかの態様において、RNAi分子は、PSMB5 DNA(例として、配列番号1のヌクレオチド450~1010)のヌクレオチド400~1010、ヌクレオチド450~1010、ヌクレオチド500~1010、ヌクレオチド550~1010、ヌクレオチド600~1010、ヌクレオチド650~1010、ヌクレオチド700~1010、ヌクレオチド750~1010、ヌクレオチド800~1010、ヌクレオチド850~1010、ヌクレオチド900~1010、またはヌクレオチド950~1010に包含される任意の配列を含むか、またはそれらからなってもよい、Coleopteran PSMB5 DNAの領域またはセグメントによってコードされるmRNAを標的とする。 In some embodiments, an RNAi molecule targeting PSMB5 comprises or consists of a sequence complementary to an mRNA encoded by a region or segment of Coleopteran PSMB5 DNA. In some embodiments, the RNAi molecule comprises nucleotides 1-500, nucleotides 10-500, nucleotides 25-500, nucleotides 50-500, nucleotides 100-500 of PSMB5 DNA (eg, nucleotides 1-450 of SEQ ID NO:1). , nucleotides 150-500, nucleotides 200-500, nucleotides 250-500, nucleotides 300-500, nucleotides 350-500, nucleotides 400-500, or nucleotides 450-500. targets the mRNA encoded by a region of Coleopteran PSMB5 DNA that may be In some embodiments, the RNAi molecule comprises nucleotides 200-950, nucleotides 250-950, nucleotides 300-950, nucleotides 350-950, nucleotides 400-950 of PSMB5 DNA (eg, nucleotides 450-927 of SEQ ID NO: 1). , nucleotides 450-950, nucleotides 500-950, nucleotides 550-950, nucleotides 200-700, nucleotides 250-700, nucleotides 300-700, nucleotides 350-700, nucleotides 400-700, nucleotides 450-700, nucleotides 500-700 , nucleotides 550-700, nucleotides 600-700, or any sequence encompassed by nucleotides 650-700. In some embodiments, the RNAi molecule comprises nucleotides 400-1010, nucleotides 450-1010, nucleotides 500-1010 , nucleotides 550-1010, nucleotides 600-1010 of PSMB5 DNA (eg, nucleotides 450-1010 of SEQ ID NO: 1). , nucleotides 650-1010, nucleotides 700-1010, nucleotides 750-1010, nucleotides 800-1010, nucleotides 850-1010, nucleotides 900-1010, or nucleotides 950-1010. targeting the mRNA encoded by a region or segment of Coleopteran PSMB5 DNA that may be
GS192:配列番号34の配列からなる1本のRNA鎖に、配列番号51の配列からなるもう1本のRNA鎖が結合している。GS192は配列番号2のヌクレオチド167~192によってコードされるmRNAを標的とする。 GS192: One RNA strand having the sequence of SEQ ID NO:34 is bound to another RNA strand having the sequence of SEQ ID NO:51. GS 192 targets the mRNA encoded by nucleotides 167-192 of SEQ ID NO:2.
Claims (19)
(a)アジュバント、誘引剤、成長調節物質、昆虫の飼料、フェロモン、タンパク質、炭水化物、ポリマー、有機化合物、生物製剤、および駆除薬からなる群から選択される少なくとも1の添加剤をさらに含む; および/または
(b)液体、溶液、懸濁液、エマルション、乳化可能な濃縮物、濃縮液、低濃縮液、超少量濃縮液、水溶性濃縮液、ベイト、逆エマルション、流動体、エアロゾル、煙、霧、流動体、均質混合物、非均質混合物、固体、粉塵、粉末、顆粒、ペレット、カプセル、燻蒸剤、カプセル製剤、またはマイクロカプセル製剤として製剤化される;および/または
(c)スプレー、霧、種子処理、ドレンチ、ドリップ灌漑、インファロー、昆虫の食餌、またはベイトとして送達される、および/または
(d) 0.0001 μg/cm 2 ~10 μg/cm 2 の濃度で製剤化された、
前記組成物。 A composition comprising the polynucleotide of any one of claims 1-12 , optionally comprising
(a) further comprising at least one additive selected from the group consisting of adjuvants, attractants, growth regulators, insect feeds, pheromones, proteins, carbohydrates, polymers, organic compounds, biologics, and pesticides; and /or
(b) liquids, solutions, suspensions, emulsions, emulsifiable concentrates, concentrates, low concentrates, ultra-low concentrates, aqueous concentrates, baits, reverse emulsions, fluids, aerosols, smoke, fog; formulated as a fluid, homogeneous mixture, heterogeneous mixture, solid, dust, powder, granules, pellets, capsules, fumigant, capsule formulation, or microcapsule formulation; and/or
(c) delivered as a spray, mist, seed treatment, drench, drip irrigation, infallow, insect food, or bait; and/or
(d) 0.0001 μg/cm 2 to 10 formulated at a concentration of μg/cm 2 ,
said composition.
(b)Coleopteran昆虫の死亡率が、Coleopteran昆虫のポリヌクレオチドへの曝露の13日未満後に、任意に未処理の条件下の対照と比べて、少なくとも45%に増加する、および/または、
(c)植物の落葉率が、Coleopteran昆虫のポリヌクレオチドへの曝露の10日未満後に、任意に未処理の条件下の対照と比べて、10%未満に減少する;またはColeopteran昆虫のポリヌクレオチドへの曝露後少なくとも10日後に、任意に未処理の条件下の対照と比べて、10%未満のままである、および/または、
(d)ポリヌクレオチドまたは組成物が、Coleopteran昆虫の発育阻止、死亡、摂食の減少、または繁殖の阻害を引き起こすために充分な量で送達される、請求項15~18のいずれか一項に記載の方法。 (a) the step of delivering the polynucleotide to the surface of the plant, to the ground, to the Coleopteran insect, or to the diet of the Coleopteran insect, at least 0.0001 μg/cm 2 , from 0.0001 μg/cm 2 to 10 including applying at a concentration of μg/cm 2 or between 0.0001 μg/cm 2 and 0.1 μg/cm 2 and/or
(b) Coleopteran insect mortality is increased by at least 45% compared to controls under optionally untreated conditions after less than 13 days of exposure to a Coleopteran insect polynucleotide, and/or;
(c) the rate of leaf defoliation of the plant is reduced to less than 10% after less than 10 days of exposure to the Coleopteran insect polynucleotide compared to controls under optionally untreated conditions; or to the Coleopteran insect polynucleotide; optionally remains less than 10% compared to controls under untreated conditions after at least 10 days of exposure to and/or
(d) the polynucleotide or composition is delivered in an amount sufficient to cause stunting, mortality, reduced feeding, or inhibition of reproduction of the Coleopteran insect; described method.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862757217P | 2018-11-08 | 2018-11-08 | |
US62/757,217 | 2018-11-08 | ||
PCT/US2019/060389 WO2020097414A1 (en) | 2018-11-08 | 2019-11-08 | Control of insect infestation |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2022507073A JP2022507073A (en) | 2022-01-18 |
JPWO2020097414A5 true JPWO2020097414A5 (en) | 2022-11-16 |
Family
ID=69160106
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2021525240A Pending JP2022507073A (en) | 2018-11-08 | 2019-11-08 | Controlling insect damage |
Country Status (8)
Country | Link |
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US (3) | US11142768B2 (en) |
EP (1) | EP3877530A1 (en) |
JP (1) | JP2022507073A (en) |
CN (1) | CN113227382A (en) |
AU (1) | AU2019377855A1 (en) |
BR (1) | BR112021009053A2 (en) |
CA (1) | CA3118148A1 (en) |
WO (1) | WO2020097414A1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3877530A1 (en) | 2018-11-08 | 2021-09-15 | Greenlight Biosciences, Inc. | Control of insect infestation |
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-
2019
- 2019-11-08 EP EP19836071.1A patent/EP3877530A1/en active Pending
- 2019-11-08 JP JP2021525240A patent/JP2022507073A/en active Pending
- 2019-11-08 WO PCT/US2019/060389 patent/WO2020097414A1/en unknown
- 2019-11-08 CA CA3118148A patent/CA3118148A1/en active Pending
- 2019-11-08 AU AU2019377855A patent/AU2019377855A1/en not_active Abandoned
- 2019-11-08 US US16/677,839 patent/US11142768B2/en active Active
- 2019-11-08 US US17/292,306 patent/US20210395738A1/en active Pending
- 2019-11-08 CN CN201980087555.5A patent/CN113227382A/en active Pending
- 2019-11-08 BR BR112021009053-3A patent/BR112021009053A2/en unknown
-
2021
- 2021-09-10 US US17/472,413 patent/US11932853B2/en active Active
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