JPS6350992B2 - - Google Patents
Info
- Publication number
- JPS6350992B2 JPS6350992B2 JP55111083A JP11108380A JPS6350992B2 JP S6350992 B2 JPS6350992 B2 JP S6350992B2 JP 55111083 A JP55111083 A JP 55111083A JP 11108380 A JP11108380 A JP 11108380A JP S6350992 B2 JPS6350992 B2 JP S6350992B2
- Authority
- JP
- Japan
- Prior art keywords
- spirulina
- solution
- algae
- nitrogen content
- liquefied
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 72
- 240000002900 Arthrospira platensis Species 0.000 claims description 65
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 65
- 239000000243 solution Substances 0.000 claims description 61
- 229940082787 spirulina Drugs 0.000 claims description 59
- 241000195493 Cryptophyta Species 0.000 claims description 49
- 239000000203 mixture Substances 0.000 claims description 37
- 229910052757 nitrogen Inorganic materials 0.000 claims description 36
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 108090000623 proteins and genes Proteins 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 239000006185 dispersion Substances 0.000 claims description 10
- 241000620196 Arthrospira maxima Species 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- -1 ll Species 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 17
- 208000035404 Autolysis Diseases 0.000 description 16
- 206010057248 Cell death Diseases 0.000 description 16
- 230000028043 self proteolysis Effects 0.000 description 16
- 230000029087 digestion Effects 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 235000019688 fish Nutrition 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 241000251468 Actinopterygii Species 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 10
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 5
- 150000001875 compounds Chemical group 0.000 description 5
- 235000002639 sodium chloride Nutrition 0.000 description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000252233 Cyprinus carpio Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000277338 Oncorhynchus kisutch Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- HDFXRQJQZBPDLF-UHFFFAOYSA-L disodium hydrogen carbonate Chemical compound [Na+].[Na+].OC([O-])=O.OC([O-])=O HDFXRQJQZBPDLF-UHFFFAOYSA-L 0.000 description 3
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 3
- 229910000397 disodium phosphate Inorganic materials 0.000 description 3
- 235000019800 disodium phosphate Nutrition 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241000277275 Oncorhynchus mykiss Species 0.000 description 2
- 241001282110 Pagrus major Species 0.000 description 2
- 241000549834 Spirulina laxissima Species 0.000 description 2
- 241000405792 Spirulina major Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- IDQFMTPFABLANH-UHFFFAOYSA-L disodium 2-aminoacetic acid chloride hydroxide Chemical compound [OH-].[Na+].[Na+].[Cl-].NCC(O)=O IDQFMTPFABLANH-UHFFFAOYSA-L 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 241000252185 Cobitidae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- RGHNJXZEOKUKBD-MGCNEYSASA-N D-galactonic acid Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-MGCNEYSASA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001124325 Marsupenaeus japonicus Species 0.000 description 1
- 241000772415 Neovison vison Species 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 241000131739 Oncorhynchus masou rhodurus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 241000202449 Sabal bermudana Species 0.000 description 1
- 241001462876 Scapania curta Species 0.000 description 1
- 241000276699 Seriola Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241001428397 Taito Species 0.000 description 1
- 241001441724 Tetraodontidae Species 0.000 description 1
- 241001504592 Trachurus trachurus Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- TUENNOFVPLQEJC-UHFFFAOYSA-L [OH-].[Cl-].[K+].[K+].NCC(O)=O Chemical compound [OH-].[Cl-].[K+].[K+].NCC(O)=O TUENNOFVPLQEJC-UHFFFAOYSA-L 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 150000004781 alginic acids Chemical group 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- NHSCRWJPZDNMBU-UHFFFAOYSA-L dipotassium carbonic acid carbonate Chemical compound [K+].[K+].OC([O-])=O.OC([O-])=O NHSCRWJPZDNMBU-UHFFFAOYSA-L 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 230000000816 effect on animals Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Description
æ¬çºæã¯æ¶²åã¹ãã«ãªããããªã溶液çµæç©ã
ãã³ãã®è£œé æ¹æ³ã«é¢ãããã®ã§ããã
ããã«è©³ããã¯ãæ¬çºæã¯åç©çšé€æã«å¯ŸããŠ
æ··åå«æµžãæãäžã«ãäœå
ã«ãããæ¶ååžåæ§ã
äžå±€åäžããæé·ä¿é²æ§ã瀺ã溶液çµæç©ããã³
ãã®è£œé æ¹æ³ã«ããããã®ã§ããã
ç·è»é¡ã®ã¯ãã¬ã©è»äœäžã«ã¯æªç¥ã®çç掻æ§ç©
質ãå«ãŸããèè»é¡ã®ã¹ãã«ãªãè»äœäžã«ãåç©
ã«å¯Ÿãæé·ä¿é²äœçšãæããæ žé
žç³»ã®æé·å åã
å«ãŸããŠãããšæšå®ãããçŸåšãããã®ç©¶æãæ¥
ãããŠããã
ã¹ãã«ãªãè»äœã¯åŒ·åºãªçŽ°èå£ãæããã¯ãã¬
ã©è»äœãšæ¯èŒããŠåç©äœå
ã«ãããŠãè»äœã«å«æ
ãããæ é€æºãä»ã®æå¹æåã®æ¶ååžåçã®ãã
ããšã¯åšç¥ã§ãããããããã¯ãã¬ã©ã«ã€ããŠã¯
çç掻æ§ç©è³ªãæœåºåé¢ããç 究ããªããããã
ã溶液ç¶æ
ã§å©çšããããšãéçºãããŠããã
äŸãã°ã¯ãã¬ã©è»äœäžã«å«ãŸããŠããæªç¥ã®ç
ç掻æ§ç©è³ªå³ã¡ãã¯ãã¬ã©ãšãã¹ã«ã€ããŠä¹³é
ž
èãåçåç©ãæ€ç©çã«æé·ä¿é²å¹æããããšç¥
ãããŠããããã®ãããªã¯ãã¬ã©ãšãã¹ã®è£œé æ³
ãšããŠã也ç¥ã¯ãã¬ã©ãç±æ°ŽæœåºåŠçããããã
ããã¯ã¢ã«ã«ãªæ°Žæº¶æ¶²ã§æœåºåŠçããæ¹æ³ããã³
å€éšããé
µçŽ ãäœçšãããŠãäŸãã°ããªãã·ã³ã
PH7.2ã8.4ã§äœçšãããããã»ã«ã©ãŒãŒãPHïŒã
ïŒã§äœçšããããããããã¯çŽè±èãããã¢ãŒãŒ
ãPHïŒã§äœçšãããŠæ¶åããåŸãæœåºåé¢ãããª
ã©ã®æ¹æ³ãç¥ãããŠããã
ããããã¹ãã«ãªãã®å Žåã«ã¯ã¯ãã¬ã©ãšåæ§
ã®æœåºåŠçãçŽã¡ã«é©çšããããšã¯å°é£ã§ããã
ããªãã¡ãæå¹æåãååŸããããã¹ãã«ãªãè»
äœãç±æ°ŽæœåºåŠçããã®ã§ã¯è±å¯ã«å«æãããã
ã¿ãã³çŸ€ã®åŠãæå¹æåãèããæžå°ãããŸãå€
éšããé
µçŽ ãäœçšãããåŠçæ¹æ³ã§ã¯é
µçŽ 補å€ã
é«äŸ¡ã§ããããæœåºæ¶²ãåç©çšäŸãã°éé¡çšã®é€
ææ·»å å€çã«é©çšã§ãããã®ã§ã¯ãªãã
æ¬çºæã®ç®çã¯è»äœäžã®ãšãã¹æåãæœåºåé¢
ããã®ã§ã¯ãªããã¹ãã«ãªãè»äœäžã«å«ãŸããæ
é€æåããã³ãã®ä»ã®æå¹æåãç·äœçã«æžå°ã
ãã«ãè»äœã®å Žåãããäžå±€æ¶ååžåæ§ãåäžã
ã液åã¹ãã«ãªããããªã溶液çµæç©ããã³ãã®
補é æ³ãæäŸããã«ããã
æ¬çºæè
ãã¯åç©çšãšãããé€æ®çšã®ä»çšéé¡
ã®ã¿ãªããæé·éçšã«ãã20ã30mm以äžã®éé¡ã«
察ããŠãã¹ãã«ãªããé€æçšã«é©å¿ãããããäœ
枩床ã§æ¶²åããç 究ãåºç¯å²ã«å€åããæ¡ä»¶äžã§
è¡ãªã€ããšãããåžžæž©è¿èŸºã®é«ã¢ã«ã«ãªæ§æ°Žæº¶æ¶²
äžã§èªå·±æ¶åããããããšã«ããç®çãéæãåŸ
ãããšãèŠåºããããŸããã¹ãã«ãªãã®å¹é€ã¯ç¡
èçã«ã¯è¡ãããŠããªãããèªå·±æ¶åã®èµ·ãããš
ã確èªããããã«éæ»
èããå¹é€æ¶²ã§çŽ«å€ç·ç
§
å°ããç¡èã®çš®ã¹ãã«ãªããå¹é€ãããããç¡è
ç®±äžã§ç¡èçã«å¥ããŠã¹ãã«ãªãã也ç¥ééæ
ç®ã§çŽïŒïœååãã0.5ã¢ã«æ¿åºŠã®çé
žãããªãŠ
ã âéçé
žãããªãŠã ã®ç·©è¡æº¶æ¶²ïŒPH10ïŒ100ml
ã«åæ£ãããã«ãšã³0.5mlãå ããŠ30âã§24æé
é眮ããããã®åæ£æ¶²ã®ïŒéšãæ¡ãé¡åŸ®é¡èŠ³å¯ã
è¡ã€ããšããã糞ç¶äœã¯å®å
šã«åæãã埮å°ãªç²
åã®ã¿ã芳å¯ãããããŸãããã®åæ£æ¶²ã®éèçœ
æ
çªçŽ å«éã®å
šçªçŽ å«éã«å¯Ÿããå²åã65ïŒ
ã瀺
ããã¹ãã«ãªããèªå·±ã®é
µçŽ ã«ããå解ãèµ·ã€ã
ããšã瀺ãããããããšåæã«éç¡èçã«å¹é€ã
ããã¹ãã«ãªãã«ã€ããŠãåæ§ã®æäœãè¡ããå
æ§ã®çµæãåŸãããã
æ¬çºæã¯ãã®ãããªç 究éçšã«ãããŠåŸããã
ç¥èŠã«åºã¥ããŠãããã®ã§ããã
æ¬çºæã«ãããŠå©çšãããã¹ãã«ãªãè»äœã«ã€
ããŠã¯ããŸããŸãªå
¬ç¥æç®ã«ãã€ãŠç¥ãããšãã§
ããã
äŸãã°ãå¹é€æ³ã«ã€ããŠã¯ç¹éæ56â64482å·
å
¬å ±ïŒçºæã®å称ïŒåŸ®çŽ°è»é¡ã®å¹é€æ¹æ³ããã³è£
眮ïŒããã³ç¹å
¬æ45â29430å·å
¬å ±ïŒçºæã®å
称ïŒè»é¡ã®å¹é€æ¹æ³ããã³å¹é€æ§œïŒèšèŒã®æ¹æ³ã«
ããããšããæ é€å¡©é¡ã溶解ããå¹é€æ¶²ã«ã¹ãã«
ãªããæ¥çš®ããŠå転äœã«ããå転åã¯çé
žã¬ã¹å«
æ空æ°ã®å¹èŸŒã¿ãé§ååã§å¹é€æ¶²ã埪ç°ããããª
ããå
ç
§å°ããããšã«ããã容æã«ã¹ãã«ãªãã
å¢æ®ãåç©«ããããšãã§ããããŸããçŸåšãã®ã
ããªå·¥æ¥çå¹é€æ³ã§å¹é€ãããŠããæ¯èŒç圢æ
ã®
倧åãªã¹ãã«ãªããšããŠã¯ãã¹ãã«ãªããã©ãã³
ã·ã¹ïŒSpirulina PlatensisïŒããã³ã¹ãã«ãªãã
ãã·ãïŒSpirulina maximaïŒã®ïŒçš®ãç¥ãããŠ
ããããã®åœ¢æ
çç¹åŸŽã®è©³çŽ°ã«ã€ããŠã¯ãç¹å
¾
50â32996å·å
¬å ±ïŒçºæã®å称ïŒéã®é£Œè²æ¹æ³ïŒ
ã«ã¿ãããšãã§ããããã®ä»ãã¹ãã«ãªãã¡ãžã€
ãŒïŒS.majorïŒãã¹ãã«ãªãããªã³ã»ã¹ïŒS.
princepsïŒãã¹ãã«ãªãã©ãã·ã·ãïŒS.
laxissimaïŒãã¹ãã«ãªãã¹ããã«ã·ãïŒS.
subtillsimaïŒãã¹ãã«ãªãã«ã«ããªã¢ïŒS.
caldariaïŒãã¹ãã«ãªãããŠã¢ã¿ïŒS.curtaïŒãã
ã³ã¹ãã«ãªãã¹ãã«ãªã·ãïŒS.spirulissimaïŒãª
ã©ãç¥ãããŠãããåŸã€ãŠã¹ãã«ãªãè»äœã®å¹é€
æ³ããã³åœ¢æ
çç¹åŸŽã«ã€ããŠãããã§ã¯è©³ããã¯
觊ããªãããçŸåšè¿ã«ç¢ºèªãããŠããã¹ãã«ãªã
è»äœäžã®æåçµæã第ïŒè¡šã«ãšããŸãšããèçœè³ª
æ§æäž»èŠã¢ããé
žçµæã第ïŒè¡šã«ç€ºããã
ãªãã第ïŒè¡šããã³ç¬¬ïŒè¡šã«ã¯æ¯èŒã®ããã«ç·
è»é¡ã®ã¯ãã¬ã©ã®çµæãä»å ããã
The present invention relates to a solution composition comprising liquefied spirulina and a method for producing the same. More specifically, the present invention relates to a solution composition that is easily mixed and impregnated into animal feed and exhibits growth-promoting properties that further improve digestibility and absorption in the body, and a method for producing the same. Chlorella, a green alga, contains unknown physiologically active substances, and Spirulina, a blue-green alga, is estimated to contain nucleic acid-based growth factors that have a growth-promoting effect on animals. There is an urgent need to investigate. It is well known that Spirulina algae have a higher rate of digestion and absorption of nutrients and other active ingredients contained in the algae in animal bodies than Chlorella algae, which have strong cell walls. However, research has been conducted to extract and separate physiologically active substances from chlorella, and the use of this in a solution state has been developed. For example, it is known that chlorella extract, an unknown physiologically active substance contained in chlorella algae, has a growth-promoting effect on lactic acid bacteria, protozoa, plants, etc. Chlorella is extracted with hot water or an alkaline aqueous solution, and an enzyme is applied externally, such as trypsin.
Either act at PH7.2~8.4 or use cellulase at PH4~
Methods are known, such as digestion with Bacillus natto protease at pH 5 or pH 7, followed by extraction and separation. However, in the case of Spirulina, it is difficult to immediately apply the same extraction process as for Chlorella.
In other words, when Spirulina algae are subjected to hot water extraction to obtain the active ingredients, the active ingredients, such as vitamins, which are abundantly contained, are significantly reduced, and when the processing method uses external enzymes, the enzyme preparations are expensive. Therefore, the extract cannot be used as a feed additive for animals, such as fish. The purpose of the present invention is not to extract and separate the extract components in the algae, but to improve the digestion of Spirulina algae without reducing the nutrients and other active ingredients contained in the algae. An object of the present invention is to provide a solution composition comprising liquefied spirulina with improved absorbability and a method for producing the same. The present inventors have made extensive changes in their research on liquefying Spirulina at low temperatures in order to adapt it for use as feed for animals, especially larval fish for aquaculture, but also for growing fish larger than 20 to 30 mm. When conducting experiments under these conditions, it was found that the objective could be achieved by autolysis in a highly alkaline aqueous solution at around room temperature. In addition, Spirulina is not cultured aseptically, but in order to confirm that autolysis occurs, we cultured sterile seed Spirulina in an oversterilized culture solution that had been irradiated with ultraviolet light, and then placed it in a sterile box. Approximately 1 g of spirulina was recovered in terms of dry weight by aseptic separation, and 100 ml of a 0.5 molar sodium carbonate-sodium bicarbonate buffer solution (PH10) was collected.
0.5 ml of toluene was added and left at 30°C for 24 hours. When a portion of this dispersion was taken and observed under a microscope, it was found that the filaments were completely separated and only minute particles were observed. Furthermore, the ratio of the non-protein nitrogen content to the total nitrogen content of this dispersion was 65%, indicating that Spirulina was degraded by its own enzyme. At the same time, similar operations were performed on Spirulina cultured in a non-sterile manner, and similar results were obtained. The present invention is based on the knowledge obtained in the course of such research. The Spirulina algae used in the present invention can be learned from various known documents. For example, regarding the culture method, JP-A-56-64482 (title of invention: method and device for culturing microalgae) and JP-B No. 45-29430 (title of invention: method and culture tank for culturing algae) are described. According to the method, spirulina can be easily grown by inoculating spirulina into a culture solution containing dissolved nutrients and irradiating it with light while circulating the culture solution using a rotating body, blowing carbon dioxide-containing air, or driving force. and can be harvested. In addition, there are two known species of relatively large Spirulina that are currently cultivated using industrial culture methods: Spirulina platensis and Spirulina maxima. For details on the characteristics of
Publication No. 50-32996 (Title of invention: Fish breeding method)
You can see it in In addition, Spirulina Major (S.major), Spirulina Princess (S.
princeps), Spirulina laxissima (S.
laxissima), Spirulina butylsima (S.
subtillsima), Spirulina Caldaria (S.
Spirulina caldaria), S. curta and S. spirulissima are also known. Therefore, we will not discuss the cultivation method and morphological characteristics of Spirulina algae in detail here, but the component composition of Spirulina algae that has been confirmed to date is summarized in Table 1, and the main amino acid composition of protein components is summarized in Table 1. It is shown in Table 2. Note that the composition of the green alga Chlorella is also added to Tables 1 and 2 for comparison.
ãè¡šããtableã
ãè¡šããtableã
ãè¡šã
æ¬çºæã«ãããŠãã¹ãã«ãªãè»äœã¯å·¥æ¥çå¹é€
ã«ããåç©«ãããäžèšã®ç¬¬ïŒè¡šããã³ç¬¬ïŒè¡šã«ç€º
ããåŠãæåçµæãæããã¹ãã«ãªãããã·ãã
ãã³ã¹ãã«ãªããã©ãã³ã·ã¹ïŒäž¡è
ã¯æåçµæé¡
䌌ïŒã®äžæ¹åã¯äž¡è
ãå©çšããã®ã奜ãŸãããã
圢æ
äžãããæåçã«é¡äŒŒãããã®ä»ã®ã¹ãã«ãª
ãå±ã®è»äœãå©çšããŠãå·®ãæ¯ããªãããããŠã
å¹é€æ§œããåååé¢åŸæ°ŽæŽããã±ãŒãç¶ã®çè»äœ
ãæã奜é©ã§ããããæ°ŽæŽåŸç 解ããããã¯ç 解
ããããšãªã也ç¥ããã¹ãã«ãªãè»äœãæãŸãã
ã¯ç±é¢šæž©åºŠ90âã200âã§åŽé§ä¹Ÿç¥ããã¹ãã«ãª
ãè»äœãããã¯æ°ŽæŽåŸåçµä¹Ÿç¥ããã¹ãã«ãªãè»
äœãªã©ããäžèšçè»äœã®äžéšãæ··å
¥ããŠå©çšã§ã
ãã
æ¬çºæã¯äžè¿°ããã¹ãã«ãªãè»äœã匷ã¢ã«ã«ãª
æ§ã®æ°Žæº¶æ¶²äžã§èªå·±æ¶åãããããã®ã§ãããã
氎溶液ã®PHå€ã¯8.0ã11.5ãšããã®ãæãŸããã
PHïŒã10ã®ç¯å²ãšããã®ãäžå±€å¥œé©ã§ããã
PHãïŒãäžåãå Žåãããã¯PHå€ã11.5ãäžå
ãå Žåã«ã¯ã¹ãã«ãªãã®èªå·±å解ã«é¢äžããé
µçŽ
ã®æŽ»æ§PHåããã¯ãããã®ã§äžé©åœã§ããã
ãã®ãããªã¢ã«ã«ãªæ§æ°Žæº¶æ¶²ã調補ããããã«
æ°Žã«æ·»å ãããååç©ãããã¯ååç©çŸ€ãšããŠã
(ã€) çé
žãããªãŠã âéçé
žãããªãŠã
(ã) çé
žã«ãªãŠã âéçé
žã«ãªãŠã
(ã) é£é
žãããªãŠã âé£é
žæ°ŽçŽ ãããªãŠã âé£é
ž
ïŒæ°ŽçŽ ãããªãŠã âæ°Žé
žåãããªãŠã
(ã) äžèš(ã)ã®ãããªãŠã å¡©ãã«ãªãŠã å¡©ãšããçµ
åãã
(ã) ã°ãªã·ã³âå¡©åãããªãŠã âæ°Žé
žåãããªãŠ
ã
(ã) ã°ãªã·ã³âå¡©åã«ãªãŠã âæ°Žé
žåã«ãªãŠã
ãªã©ã®äžçš®åã¯äºçš®ä»¥äžãå©çšããããšãã§ã
ãããŸããäžèšååç©åã¯ååç©çŸ€ã®æ°Žæº¶æ¶²äžã®
æ¿åºŠã¯ã0.05ã1MïŒãšããã®ãæãŸãããPH
ã¯æ°Žé
žåãããªãŠã ãªã©ã®æ°Žé
žåç©ã§èª¿æŽããã
ãã®ãããªã¢ã«ã«ãªæ°Žæº¶æ¶²ã«å¯Ÿããã¹ãã«ãªã
è»äœã®èªå·±æ¶åä¿é²ããã³ïŒãŸãé²è
ãããã¯æ
èãç®çãšããŠäžèšã®ååç©ã®äžçš®åã¯äºçš®ä»¥äž
ãã¢ã«ã«ãªæ°Žæº¶æ¶²ã«å¯ŸããŠ(a)ã(e)ã«ã€ããŠã¯ãïŒ
ã10MolïŒã(f)ã«ã€ããŠã¯0.05ãïŒïŒ
æ¿åºŠãšãª
ãããã«æ·»å ããã®ã奜é©ã§ããã
(a) å¡©åãããªãŠã ãå¡©åã«ãªãŠã ã®åŠãç¡æ©å¡©
é¡ã
(b) 庶ç³ãæç³ãä¹³ç³ããããŠç³ãã¬ã©ã¯ããŒ
ã¹ããœã«ããŒã¹ããã«ããŒã¹ã®åŠãç³é¡ã
(c) ãœã«ãããŒã«ããã³ãããŒã«ã®åŠãç³ã¢ã«ã³
ãŒã«é¡ã
(d) ã°ã«ã³ã³é
žãã¬ã©ã¯ãã³é
žãä¹³é
žãé
¢é
žãã
ãããªã³é
žãã¯ãšã³é
žããªã³ãŽé
žããããŒã«é
ž
ã®åŠãææ©é
žããã³ãã®å¡©é¡ã
(e) ã°ã«ã¿ãã³é
žãã¢ã¹ãã©ãã³é
žãã¢ã©ãã³ã
ãªãžã³ã®åŠãã¢ããé
žåã³ãã®å¡©é¡ã
(f) ãã«ãšã³ãé
¢é
žãšãã«ããã·ã¬ã³ãïœâãã
ãµã³ããšãŒãã«ããã³ãŒã³ã®åŠãçæ°Žæ§ææ©æº¶
åªã
æ¬çºæã«ãããŠã¯ã¹ãã«ãªãè»äœãã¢ã«ã«ãªæ°Ž
溶液ïŒåœã也ç¥ééã§10ã100ïœæ·»å åæ£ãã
枩床25ã55âãæãŸããã¯30ã45âã«ä¿æããªã
ãæ¹æåã¯æ¹æããã«0.5ã48æéã«ãŠèªå·±æ¶å
ãããããèªå·±æ¶åæã®æ¶²æž©ã25âãäžåãå Žå
ã«ã¯æ¶åã®é²è¡ãç·©æ
¢ã§äžé©åœã§ããã55âãäž
åãå Žåã¯é
µçŽ 倱掻ãèµ·ãäžé©åœã§ãããèªå·±æ¶
åå®äºåŸãã®ãŸãŸã®ç¶æ
ãããã¯çšéç®çã«å¿ã
ãŠé±é
žãããã¯ææ©é
žã§PHã®èª¿æŽãè¡ãæ¬çºæã®
ç®ç補åãåŸãããã
æ¬çºæã®æº¶æ¶²çµæç©ã¯ãé®å
æ§å¯æ 容åšã«ä¿å
ããã°é·æéå€è³ªããããšã¯ãªãã
æ¬çºæã«ãããã¹ãã«ãªãè»äœæ¶²åç©ã«ãã
ãŠãè»äœã®èªå·±æ¶åçšåºŠã¯äŸãã°éèçœæ
çªçŽ å«
éãçµæçã«å®éåæããããšã«ãã€ãŠææ¡ãã
ããšãã§ãããå³ã¡ãã¹ãã«ãªãè»äœäžã«ã¯ãå¹
é€æ¡ä»¶ã«ãã€ãŠå€å°ã®å€åã¯ããããéåžžçŽ10ã
11ïŒ
ã®çªçŽ ãå«ãŸãããã®å
šçªçŽ ã100ãšããŠçŽ
2.8ïŒ
çšåºŠã®éèçœæ
çªçŽ ãå«æãããèªå·±æ¶å
ã®é²è¡ãšå
±ã«éèçœæ
çªçŽ å«éãå¢å€§ããã®ã§æ¶
åçšåºŠã容æã«ææ¡ã§ãããäŸãã°ãèªå·±æ¶åé
å§åŸçŽ42æéçµéããŠã»ãŒæ¶åé²è¡çšåºŠã®éåã
ãã¹ãã«ãªãè»äœæ¶²åç©ã®å
šçªçŽ å«éã«å¯Ÿããé
èçœæ
çªçŽ å«éã®å²åã42.2ïŒ
ã§ããããšã確èª
ãããŠãããããã¯èçœæ
çªçŽ ãé
µçŽ å解äœçšã«
ããèªå·±æ¶åããå²åã40.5ïŒ
ã§ããããšã瀺ã
ãŠããããã®ããã«é«åºŠã«èçœè³ªãå解ããã溶
液ã¯ãé
µçŽ 掻æ§ã倱ã€ãè»äœããã§ã¯å·æ°Žåã¯ç±
æ°Žæœåºãããã¯åŒ·ã¢ã«ã«ãªæœåºïŒPH11.5çšåºŠïŒã§
ãå°åºåŸãããšã¯ã§ããªãã
æ¬çºæã«ãããå
šçªçŽ å«éã¯äžèšæº¶æ¶²å
šå
容ç©
ãéåžžã®ã±ã«ããŒã«çªçŽ ãšããŠæ±ãããã®ã§ã
ããéèçœæ
çªçŽ ã¯10ïŒ
æ¿åºŠã®ããªã¯ãã«é
¢é
žã«
å¯æº¶ãªçªçŽ ãã±ã«ããŒã«æ³ã§æ±ãããã®ã§ããã
ãªããã¹ãã«ãªãã®è¢«åæ£åªäžã«ã°ãªã·ã³ãå°¿
çŽ çã®çªçŽ ååç©ãæ·»å ãããšå
šçªçŽ å«éå¯æº¶æ§
çªçŽ å«éãšã倧ããäžãåºãããã®ã§åãã€ãŠã
ã®åã®çªçŽ å«éãæ±ããŠãããã¹ãã«ãªãã®ã¿ã«
ç±æ¥ããäžè¿°ã®åçªçŽ å«éãç®åºããã®æ¯ãæ±ã
ãã
æ¬çºæã«ãããã¹ãã«ãªãè»äœæ¶²åç©ã¯å
šçªçŽ
äžã«å ããéèçœæ
çªçŽ ã®å²åãå°ããšã20ïŒ
ã
æãŸããã¯30ïŒ
以äžã§ããããšãèèŠã§ããã20
ïŒ
ã®å€ãäžåãå Žåã¯ã¹ãã«ãªãè»äœã®å€§ããªæ
çã液åç©äžã«ååšããŠããã®ã§äŸãã°ä»çšéçš
ã«ã¯å¥œãŸãããªãããããŠãå
šçªçŽ äžã®éèçœæ
çªçŽ ã®å²åã30ïŒ
ãäžåããšæ¶²åç©äžã«ã¯å€§ããª
æçïŒçŽ10ÎŒïœä»¥äžïŒã¯èŠåœããªããªãã
ããã«æ¬çºæã«ãããŠè»äœã®èªå·±æ¶åçšåºŠã¯ã
æ¶åã«ãšããªã€ãŠæ¶²äžã«æº¶åºéé¢ããŠããç©è³ªã
å
åŠçã«æž¬å®ããããšã«ãã€ãŠãææ¡ããããšã
ã§ãããå³ã¡ãæ¬çºæè
ããæ žé
žç³»ã®ç©è³ªã§ãã
ãšæšå®ããŠãã260nïœä»è¿ã«æ¥µå€§åžåã®ãã¿ãŒ
ã³ã瀺ãç©è³ªãååšãããã®260nïœã«ãããåž
å
床ã®å¢å€§çšåºŠã枬å®ããããšã«ããææ¡ããã
ãšãã§ãããäŸãã°ãã¹ãã«ãªãè»äœã®äžå®éã
äžå®éã®æ¶å液äžã§äžè¿°ã®æ¹æ³ã«ããèªå·±æ¶åã
ã溶液ã«æçµæ¿åºŠã0.4èŠå®ãšãªãããã«éå¡©çŽ
é
žãæ·»å ããçæããå¯æº¶æ§èçœã®ååºæ²æŸ±ãšè»
äœæ®æž£ãé å¿åé¢æ©ïŒ10000Gã10åéïŒãçšã
ãŠåé¢é€å»ããåŸããã溶液ãåå
å
床èšã«ãã
ãŠ260nïœã®åžå
床ã枬å®ãããããããå解床
ãšåžå
床ã®é¢ä¿ãæ±ããŠãããæ€éç·ããå解çš
床ãææ¡ããããšãã§ããã
æ¬çºæã«ããã溶液çµæç©ã¯ãåç©çšãšããã
éé®ãçé¯ãé¯ãã©ããããã²ãããã«ããŸãã
ããŸãããµãããããã¯ãŸã¡ãããã±ã¡ããŠããŽ
ãããããããªãããªã©ã®é€æ®çšä»çšéã®é€æã«
å©çšããã®ã奜é©ã§ãããã20ã30mm以äžã®æé·
éçšã«ããåçš®é€æ®éé¡ã«ãå©çšã§ããã
ããã«ãæ¬çºæã«ããã溶液çµæç©ã¯äžè¿°ãã
éé¡çšã®é€æçŽ æãšããŠå©çšã§ããã°ããã§ã¯ãª
ããä»ã®åç©çšäŸãã°çãè±ã銬ãçŸããã³ã¯ã
å±±çŸã®åŠã家çãå®éšåç©ïŒã¢ã«ã¢ãããããº
ãïŒããã³ã«ããšããå°é³¥ã®åŠã家é¢é¡ãããã«
ã¯ã¿ã©ãããããããããçã®åçåç©ããã
é¡ãã¿ãããããããŸãã³çã®ç²æ®»é¡ã®é€æãšã
ãŠãé©å®å©çšããããšãã§ããããšãããããã
ãåç©ã®å¹Œçæã®åºåœ¢é€æã液ç¶é€æã«æ·»å ããŠ
å©çšããã®ã奜é©ã§ããã
æ¬çºæçµæç©ã¯ãé€æã«å¯ŸããŠåŽé§ã»å«æµžã»æµž
挬ã»æ··åãªã©ã®æ¹æ³ã§æ·»å ã§ããããèŠã¯é€ã«å
äžã«åæ£æ·»å ããåã
ã®åç©ãé€æ®éã«å¹çãã
æäžãæé€ãããéãåŠäœãªãæ·»å æ¹æ³ãæ¡çšã§
ãããç¹ã«åååã²ã«ãã€ã³ããŒïŒå接ååŠæ ªåŒ
äŒç€Ÿè£œïŒãã€ãªããïŒç°èŸºè£œè¬æ ªåŒäŒç€Ÿè£œïŒãã¹
ã¿ã³ã¬ãŒãïŒå°ç³ãã¢ã€ã¶ãŒæ ªåŒäŒç€Ÿè£œïŒã®åŠã
ã¢ã«ã®ã³é
žãäž»æåãšããé€æçšå±çå€ãšæº¶æ¶²çµ
æç©ã®æ··å溶液ãé€æã«å¯ŸããŠåŽé§ãããã¯æ··å
ããŠå©çšããã®ã奜é©ã§ããã
以äžã®æ¬çºæã«ããã°ã¹ãã«ãªãè»äœã«å«ãŸã
ãæå¹æåãã»ãšãã©æº¶æ¶²äžã«æº¶åããŠããç¶æ
ã®æº¶æ¶²çµæç©ãåŸãããã®ã§ãè»äœãã®ãŸãŸã®å Ž
åãããåç©ã«å¯Ÿããæ¶ååžåæ§ã¯æ Œæ®µã«åäžã
ããäžã€æº¶æ¶²ç¶æ
ã§ããããåºç€é€æã«å¯Ÿããæ·»
å ã®éã®åæ±ããäžå±€å®¹æã§ãããšããå©ç¹ãã
ãã
以äžã«å®æœäŸããã³æº¶æ¶²çµæç©ã®äœ¿çšäŸã瀺
ããæ¬çºæãããã«è©³ãã説æããã
å®æœäŸ ïŒ
å¹é€æ§œããæ¡ååŸãè±æ°Žããã¹ãã«ãªãããã·
ãã®çè»äœãããªãã±ãŒãïŒä¹Ÿç¥ééæç®30ïœïŒ
ã20ééïŒ
ã®é£å¡©ãšãããªãŠã æ¿åºŠã§ãã¢ã«ãšãª
ãããã«çé
žãããªãŠã âéçé
žãããªãŠã ãæ·»
å ãã氎溶液ïŒPH10ïŒã«åæ£ããããã®åæ£æº¶æ¶²
ã枩床30âã«å¶åŸ¡ããªãã48æéä¿æãè»äœãèª
å·±æ¶åãããããåæ£æº¶æ¶²ã®çµæçå€åãé¡åŸ®é¡
芳å¯ãããšãããçŽ18æéåŸã«ã¯ã¹ãã€ã©ã«ç¶ã®
圢æ
ã¯å¯žæãããçŽ26æéåŸã«ã¯äžèŠéã«ïŒåäœ
现èãããªãæçãïŒãïŒåæ®åããçšåºŠã«æ¶å
ãé²è¡ãã40æéåŸã«ã¯ãããã®æçãæ¶å€±ãã
çšåºŠã«æ¶åã®é²è¡ããããšãèªããããããŸãã
48æéåŸã«ã¯å
šçªçŽ å«éã«å¯Ÿããéèçœæ
çªçŽ å«
éã®å²åã45ïŒ
ãšãªã€ããããã«ãæ¶ååŠçåŸã®
溶液ã«æçµæ¿åºŠã0.4èŠå®ãšãªãããã«éå¡©çŽ é
ž
ãæ·»å ããŠå¯æº¶æ§èçœãååºããåŸé å¿åé¢æ©
ïŒ10000GïŒã«10åéãããŠãè»äœæ®æž£ãšåèšååº
æ²æŸ±ãé€å»ããåŸãæ³¢é·220nïœãã350nïœã®åž
å
床ã枬å®ãããšãã260nïœã«åžå極倧ãæã
ã第ïŒå³ã«ç€ºãããšããã®æ²ç·ãåŸãããããª
ãããã®260nïœã®åžå
床ïŒã»ã«åïŒïŒcmïŒãè»
äœã®èªå·±æ¶åæéã®çµéãšå
±ã«äžè¿°ããæ¹æ³ã«æº
ããŠæž¬å®ãããšãã第ïŒå³ã«ç€ºãããšããã®æ²ç·
ãåŸããããïœãã®å Žå也ç¥äœæç®ã§ïŒïœã®ã¹ã
ã«ãªãã100mlã«åæ£ãèªå·±æ¶åãããå Žåã«æ
ç®ãããšO.D.260nïœïŒïŒcmã»ã«ïŒã¯12.5ãšãªã
æ¯èŒäŸ ïœ
åæ£æº¶æ¶²ã®PHãïŒã«å¶åŸ¡ãã溶液(ã€)ããã³PHã
12ã«å¶åŸ¡ãã溶液(ã)ã調補ãã以å€ã¯ãå®æœäŸïŒ
ã®æäœãããè¿ããã
溶液(ã€)ããã³æº¶æ¶²(ã)ã®äž¡è
å
±ã«æ¶åã®é²è¡ãç·©
æ
¢ã§ã48æéåŸã®æ¶åé²è¡çšåºŠãå®æœäŸïŒã®å Žå
ã®10æéçµéåŸã®ãã®ã«çžåœããå®çšæ§ã®ãã補
é æ³ãšã¯èªããããªãã€ãã
å®æœäŸ ïŒ
å¡©åã«ãªãŠã 200ïœãšçé
žæ°ŽçŽ ãããªãŠã
ïŒNa2HPO4ã»12H2OïŒ18ïœãæ°Žã«æº¶è§£ãããã
ã«æ°Žã§ããããŠïŒãšããåŸ10èŠå®ã®æ°Žé
žåãã
ãªãŠã 溶液ãæ·»å ããŠPH10ã«èª¿æŽããããã®æº¶æ¶²
ã«ã¹ãã«ãªããã©ãã³ã·ã¹ã®çè»äœãããªãã±ãŒ
ãïŒä¹Ÿç¥ééæç®60ïœïŒãæ·»å åæ£ãã枩床ã35
âã«å¶åŸ¡ããªãã24æéä¿æãèªå·±æ¶åããã
ãã
ãŸããæ¶åé²è¡ãšå
±ã«æã
PHã枬å®ããïŒèŠå®
ã®æ°Žé
žåãããªãŠã ã滎äžããŠPHã10ã«ä¿æã
ãããã®æº¶æ¶²çµæç©ã®å
šçªçŽ å«éã«å¯Ÿããéèçœ
æ
çªçŽ å«éã®å²åã¯41ïŒ
ã§ãã€ãã
å®æœäŸ ïŒ
çé
žæ°ŽçŽ ãããªãŠã ïŒNa2HPO4ã»12H2OïŒ18
ïœã400mlã®æ°Žã«æº¶è§£ããåŸã庶ç³825ïœãå ããŠ
å 枩溶解ãã10èŠå®ã®æ°Žé
žåãããªãŠã 溶液ãæ·»
å ããŠPHã10ãšããããã®æº¶æ¶²ã«ã¹ãã«ãªããã
ã·ãã®çè»äœãããªãã±ãŒãïŒä¹Ÿç¥ééæç®25
ïœïŒãšã¹ãã«ãªããã©ãã³ã·ã¹ã®çè»äœãããªã
ã±ãŒãïŒä¹Ÿç¥ééæç®25ïœïŒãšãå ããŠåæ£æ··å
ãã枩床ã35âã«å¶åŸ¡ããªãã48æéä¿æããèª
å·±æ¶åããããããã®æº¶æ¶²çµæç©ã®å
šçªçŽ å«éã«
察ããéèçœæ
çªçŽ å«éã®å²åã¯38ïŒ
ã§ãã€ãã
æ¯èŒäŸ ïœ
å®æœäŸïŒã®æäœãããè¿ãããäœããåæ£æº¶æ¶²
ã®æž©åºŠã13âïŒæ¡ä»¶âïŒïŒããã³å枩床ã57â
ïŒæ¡ä»¶âïŒïŒã«å¶åŸ¡ããã¹ãã«ãªãè»äœã®èªå·±æ¶
åæ¡ä»¶ã®ã¿ãå€åããã
æ¡ä»¶âïŒã®å Žåã¯48æéçµéåŸã®æº¶æ¶²çµæç©äž
ã®å
šçªçŽ å«éã«å¯Ÿããéèçœæ
çªçŽ å«éã®å²åã
çŽ10ïŒ
ã§æ¶åé²è¡ã極ããŠç·©æ
¢ã§ãã€ãã
ãŸãæ¡ä»¶âïŒã®å Žåã¯ïŒæéåŸã®åçªçŽ å«éã®
å²åãçŽ15ïŒ
ã«éããããã©ãããã®åŸã®å¢å ã
èªããããèªå·±æ¶åã«å¿
èŠãªé
µçŽ ã®å€±æŽ»ããããš
ãèªããããã
å®æœäŸ ïŒãïŒ
被åæ£æº¶æ¶²çµæãã¹ãã«ãªãè»äœããã³æž©åºŠã»
ä¿ææéçã®èªå·±æ¶åæ¡ä»¶ã®çµåããã第ïŒè¡šã«
瀺ããåŠãå€åããæ¬çºæã®æ¶²åã¹ãã«ãªã溶液
çµæç©ã補é ããã[Table] In the present invention, Spirulina algae is one of Spirulina maxima and Spirulina platensis (both have similar compositions) having the composition shown in Tables 1 and 2 above, which are harvested by industrial cultivation. Or it is preferable to use both,
There is no problem in using other algal bodies of the genus Spirulina that are more similar in composition than in morphology. and,
Cake-like living algae collected from the culture tank, separated and washed with water are most suitable, but Spirulina algae that are washed with water and then crushed or dried without being crushed, preferably spray-dried at a hot air temperature of 90°C to 200°C. Spirulina algae that have been washed or freeze-dried after washing with water can also be used by mixing a part of the above-mentioned live algae. The present invention is made by autolyzing the above-mentioned Spirulina algae in a strongly alkaline aqueous solution.
The pH value of the aqueous solution is preferably 8.0 to 11.5.
More preferably, the pH is in the range of 9 to 10. If the PH value is less than 8 or more than 11.5, it is inappropriate because it is out of the active PH range of enzymes involved in the autolysis of Spirulina. Compounds or compound groups added to water to prepare such an alkaline aqueous solution include (a) Sodium carbonate - sodium bicarbonate (b) Potassium carbonate - potassium bicarbonate (c) Sodium phosphate - sodium hydrogen phosphate - Sodium dihydrogen phosphate - Sodium hydroxide (d) A combination of the above (c) sodium salt and potassium salt. (e) Glycine-sodium chloride-sodium hydroxide (f) One or more of glycine-potassium chloride-potassium hydroxide can be used. In addition, the concentration of the above compound or compound group in the aqueous solution is preferably 0.05 to 1M/PH
is adjusted with hydroxide such as sodium hydroxide. Regarding (a) to (e), one or more of the following compounds are added to such an alkaline aqueous solution for the purpose of promoting self-digestion of Spirulina algae and/or for antiseptic or antibacterial purposes.
~10Mol/, and (f) is preferably added at a concentration of 0.05 to 5%. (a) Inorganic salts such as sodium chloride and potassium chloride. (b) Sugars such as sucrose, fructose, lactose, glucose, galactose, sorbose, and maltose. (c) Sugar alcohols such as sorbitol and mannitol. (d) Organic acids and their salts such as gluconic acid, galactonic acid, lactic acid, acetic acid, propionic acid, citric acid, malic acid, fumaric acid. (e) Glutamic acid, aspartic acid, alanine,
Amino acids such as lysine and their salts. (f) Hydrophobic organic solvents such as toluene, ethyl acetate, xylene, n-hexane, ether, benzene. In the present invention, spirulina algae are added and dispersed in a dry weight of 10 to 100 g per 1 alkaline aqueous solution,
Autolysis is carried out for 0.5 to 48 hours with or without stirring while maintaining the temperature at 25 to 55°C, preferably 30 to 45°C. If the temperature of the solution during autolysis is lower than 25°C, the digestion progresses slowly, which is inappropriate; if it exceeds 55°C, enzyme deactivation occurs, which is inappropriate. After completion of autolysis, the desired product of the present invention can be obtained by adjusting the pH with mineral acid or organic acid depending on the intended use or as it is. The solution composition of the present invention will not deteriorate over a long period of time if stored in a light-shielding sealed container. In the liquefied Spirulina algae according to the present invention, the degree of autolysis of the algae can be determined, for example, by quantitatively analyzing the non-protein nitrogen content over time. In other words, although there is some variation depending on the culture conditions, the number of Spirulina algae is usually about 10 to 10.
Contains 11% nitrogen, taking this total nitrogen as 100.
It contains about 2.8% non-protein nitrogen, and since the non-protein nitrogen content increases as autolysis progresses, the degree of digestion can be easily determined. For example, it has been confirmed that the ratio of non-protein nitrogen content to the total nitrogen content of liquefied Spirulina algae, which has slowed down to the extent of digestion after about 42 hours had passed since the start of autolysis, was 42.2%. This indicates that 40.5% of protein nitrogen was self-digested by enzymatic decomposition. Such a highly decomposed solution of proteins cannot be obtained from algae that have lost enzymatic activity by cold water or hot water extraction, or by strong alkaline extraction (pH around 11.5). The total nitrogen content in the present invention is determined by calculating the total content of the solution as normal Kjeldahl nitrogen, and the non-protein nitrogen is determined by calculating the nitrogen soluble in 10% trichloroacetic acid by the Kjeldahl method. Note that when nitrogen compounds such as glycine and urea are added to the Spirulina dispersion medium, both the total nitrogen content and the soluble nitrogen content will be greatly increased. Calculate each nitrogen content and find the ratio. The liquefied Spirulina algae body according to the present invention has a proportion of non-protein nitrogen in the total nitrogen of at least 20%,
It is important that it is preferably 30% or more, and 20
If the value is less than 20%, large fragments of Spirulina algae are present in the liquefied product, which is not preferable for use in, for example, larvae. When the proportion of non-protein nitrogen in the total nitrogen exceeds 30%, no large fragments (about 10 ÎŒm or more) are found in the liquefied product. Furthermore, in the present invention, the degree of autolysis of algae is
It can also be determined by optically measuring the substances that are eluted and liberated into the liquid during digestion. That is, there is a substance that exhibits a maximum absorption pattern around 260 nm, which the present inventors estimate is a nucleic acid-based substance, and this can be determined by measuring the degree of increase in absorbance at 260 nm. For example, perchloric acid is added to a solution in which a certain amount of Spirulina algae is autolysed in a certain amount of digestive fluid by the method described above so that the final concentration is 0.4N, and the resulting coagulation precipitate of soluble protein and algae are The body residue was separated and removed using a centrifuge (10000G, 10 minutes), and the resulting solution was measured with a spectrophotometer to measure the absorbance at 260 nm, and the relationship between decomposition and absorbance was determined from a calibration curve that had been determined in advance. The degree of decomposition can be grasped. The solution composition according to the present invention can be used for animals, especially coho salmon, red sea bream, carp, Japanese loach, flounder, and rainbow trout.
It is suitable for use as feed for aquaculture larvae such as amago, blowfish, horse mackerel, yellowtail, amberjack, telapia, ayu, eel, etc., but it can also be used for various aquaculture fish that are in the growth stage of 20 to 30 mm or more. can. Furthermore, the solution composition according to the present invention can be used not only as a feed material for the above-mentioned fish, but also for other animals such as cows, pigs, horses, sheep, mink, etc.
Appropriately used as feed for livestock such as goats, experimental animals (guinea pigs, rats), and stray animals such as chickens and small birds, as well as protozoan rotifers such as green beetles and gourd bugs, and crustaceans such as watermelons and Kuruma shrimp. can be used. In particular, it is suitable to use it by adding it to solid feed or liquid feed for the larval stage of these animals. The composition of the present invention can be added to feed by methods such as spraying, impregnating, dipping, and mixing, but the important point is that as long as it is evenly dispersed and added to feed and efficiently administered to and ingested by individual animals and farmed fish. Any method of addition can be used. In particular, a mixed solution of a feed spreader and a solution composition whose main component is alginic acid, such as Gel Binder (manufactured by Kimitsu Chemical Co., Ltd.), Mailitschi (manufactured by Tanabe Seiyaku Co., Ltd.), and Stanguard (manufactured by Taito Pfizer Co., Ltd.). It is preferable to use it by spraying or mixing it with the feed. According to the present invention as described above, a solution composition in which most of the active ingredients contained in Spirulina algae are dissolved in the solution can be obtained, so that the digestibility for animals is significantly improved compared to when the algae are intact. Moreover, since it is in a solution state, it has the advantage of being easier to handle when added to basic feed. The present invention will be explained in more detail by showing Examples and usage examples of solution compositions below. Example 1 A cake made of living Spirulina maxima algae collected from the culture tank and dehydrated (30 g in dry weight)
was dispersed in an aqueous solution (PH10) containing 20% by weight of common salt and sodium carbonate-sodium bicarbonate so that the sodium concentration was nomolar. This dispersion solution was kept at a controlled temperature of 30°C for 48 hours to allow the algae to self-digest. When we observed changes in the dispersion solution over time using a microscope, we found that the spiral shape was fragmented after about 18 hours, and after about 26 hours, digestion had been completed to the extent that 4 to 5 fragments of one unit cell remained in one field of view. Digestion progressed to the extent that these fragments disappeared after 40 hours. Also,
After 48 hours, the ratio of non-protein nitrogen content to total nitrogen content was 45%. Furthermore, perchloric acid was added to the solution after the digestion treatment to a final concentration of 0.4N to coagulate the soluble proteins, and then centrifuged (10,000G) for 10 minutes to separate the algae residue and the coagulated precipitate. After removal, the absorbance was measured at wavelengths from 220 nm to 350 nm, and a curve as shown in FIG. 1 was obtained, having an absorption maximum at 260 nm. When the absorbance at 260 nm (cell thickness: 1 cm) was measured as the autolysis time of the algae increased according to the method described above, a curve as shown in FIG. 2 was obtained. {In this case, when 1 g of spirulina is dispersed in 100 ml in terms of dry matter and autolyzed, the OD260nm (1 cm cell) is 12.5. Comparative example a Solution in which the pH of the dispersion solution was controlled to 7 (a) and PH of
Example 1 except that a solution (b) controlled at 12 was prepared.
The operation was repeated. Digestion progressed slowly in both solution (a) and solution (b), and the degree of digestion progress after 48 hours was equivalent to that after 10 hours in Example 1, indicating that the production method is practical. was not recognized. Example 2 200 g of potassium chloride and 18 g of sodium hydrogen phosphate (Na 2 HPO 4 .12H 2 O) were dissolved in water, further diluted with water to a pH of 1, and 10N sodium hydroxide solution was added to adjust the pH to 10. did. Add and disperse a cake made of living Spirulina platensis algae (60 g in dry weight) to this solution, and reduce the temperature to 35
The mixture was maintained at a controlled temperature for 24 hours to allow autolysis. Additionally, as the digestion progressed, the pH was measured from time to time, and 5N sodium hydroxide was added dropwise to maintain the pH at 10. The ratio of non-protein nitrogen content to the total nitrogen content of this solution composition was 41%. Example 3 Sodium hydrogen phosphate ( Na2HPO4ã»12H2O ) 18
After dissolving 825 g of sucrose in 400 ml of water, 825 g of sucrose was added and dissolved by heating, and the pH was adjusted to 10 by adding 10N sodium hydroxide solution. Add this solution to a cake consisting of living algae of Spirulina maxima (25% on dry weight basis).
g) and a cake made of living algae of Spirulina platensis (25 g in terms of dry weight) were added and dispersed and mixed, and the temperature was controlled at 35° C. and maintained for 48 hours to allow self-digestion. The ratio of non-protein nitrogen content to the total nitrogen content of this solution composition was 38%. Comparative Example b The operation of Example 3 was repeated. However, the temperature of the dispersion solution is 13â (condition-1) and the same temperature is 57â.
(Condition-2), and only the autolysis conditions of Spirulina algae were changed. In the case of condition-1, the ratio of non-protein nitrogen content to the total nitrogen content in the solution composition after 48 hours was approximately 10%, and the progress of digestion was extremely slow. Further, in the case of condition-2, although the nitrogen content ratio reached approximately 15% after 6 hours, no increase was observed after that, indicating that the enzyme necessary for autolysis had been deactivated. Examples 4 to 8 Dispersed solution composition, Spirulina algae and temperature/
The liquefied spirulina solution composition of the present invention was produced by changing the combination of autolysis conditions such as retention time as shown in Table 3.
ãè¡šã
ããã
䜿çšäŸ ïŒ
å®æœäŸïŒã§è£œé ããæ¬çºæ溶液çµæç©ã«å¡©é
žã
å ããŠã»ãŒäžæ§ã«äžåãããã®æ¶²å
šäœã次ã®é£Œè²
å®éšã«äœ¿çšããã察象éã¯éé®å¹Œéãšããäžåºåœ
ãçŽ25000å°Ÿã90m2氎槜ã«æ³šæ°Žéæ¯å2.5tã§é£Œè²
ãããåºç€é£Œæã«ã¯åååããžãã¹çšãã¬ãã
ïŒæåç£æ¥æ ªåŒäŒç€Ÿè£œïŒãçšãããã¬ããããã
ãµãŒäžã§æ¹æããªãã察ç
§åºã®é£Œæã«ã¯é£Œæçšå±
çå€æ°Žæº¶æ¶²ããã³å®æœäŸïŒã«çšããçµæã®ã¢ã«ã«
ãªæº¶æ¶²ã®äžå液ããè©Šéšåºã®é£Œæã«ã¯é£Œæçšå±ç
å€æ°Žæº¶æ¶²ãšæ¬çºæ溶液çµæç©ã®æ··å溶液ã墳é§åš
ã§åäžã«ã¹ãã¬ãŒãããã®åŸãã€ãŒããªã€ã«ãäž¡
åºã®é£Œæã«æ·»å åžåãããŠèª¿é€ãããå䜿çšææ
æåã®é
åå²åã¯æ¬¡ã®ç¬¬ïŒè¡šã®éãã§ããã[Table] Yes.
Usage Example 1 Hydrochloric acid was added to the solution composition of the present invention produced in Example 1 to neutralize it to almost neutrality, and the entire solution was used in the next breeding experiment. The target fish were young coho salmon, and approximately 25,000 fish per section were raised in a 90 m 2 tank at a water injection rate of 2.5 tons per minute. Pellets for rainbow trout (trade name, manufactured by Showa Sangyo Co., Ltd.) were used as the basic feed, and while the pellets were stirred in a mixer, an aqueous feed spreader solution and an alkaline solution with the composition used in Example 1 were added to the control feed. The neutralizing solution was prepared by uniformly spraying a mixed solution of a feed spreader aqueous solution and the solution composition of the present invention on the feed in the test group using a sprayer, and then adding feed oil to the feed in both groups and allowing it to be absorbed. I fed it. The blending ratio of each material component used is as shown in Table 4 below.
ãè¡šããtableã
ãè¡šã
絊é€çã¯1.3ïŒ
èŠåœãšãæ«æ絊é€éãå¢å ãã
ãã絊é€ã¯å¯Ÿç
§åºè©Šéšåºãšãåéã®ãã¬ããã«ã
ã€ãŒããªã€ã«ïŒåååçç ãã€ãŒããªã€ã«Î©ïŒç
ç ãã¿ãã³æ ªåŒäŒç€Ÿè£œïŒãå«æµžãããŠæäžããã
è©Šéšçµæã¯ç¬¬ïŒè¡šã®ãšããã§ãã€ãã
第ïŒè¡šäžã®å¯Ÿç
§åºâïŒã¯ã第ïŒè¡šã®è©Šéšåºé€æ
äžã®æ¶²åã¹ãã«ãªã溶液çµæç©100éééšã®ãã
ãã«ã¹ãã«ãªãããã·ãçè»äœã也ç¥ééæç®ã§
ïŒéééšãé
åãã以å€ã¯åçµæã®é£Œæã調補ã
ãŠé£Œè²è©Šéšã«äŸããçµæã§ããã[Table] The feeding rate was set at 1.3% and the feeding amount was increased temporarily. For feeding, the same amount of pellets was impregnated with feed oil (trade name: Riken Feed Oil Ω, manufactured by Riken Pittamin Co., Ltd.) and administered to both the control and test plots. The test results were as shown in Table 5. Control group-2 in Table 5 is the same except that instead of 100 parts by weight of the liquefied Spirulina solution composition in the test group feed in Table 4, 3 parts by weight of Spirulina maxima live algae was added in terms of dry weight. These are the results of preparing a feed with the same composition and subjecting it to a feeding test.
ãè¡šã
ãªããäžè¿°ã®æ¯èŒäŸâ(b)ã®æ¡ä»¶âïŒã§èª¿è£œãã
èªå·±æ¶å液ïŒéèçœæ
çªçŽ å«éïŒå
šçªçŽ å«éïŒ
0.15ïŒãæ¬äœ¿çšäŸãšåæ§ã«éé®é£Œè²ã«é©çšãã
ãã察ç
§åºâïŒãšåçšåºŠã®çµæããåŸãããªãã€
ãã
飌è²æ¥æ°ïŒïœïŒ 36æ¥ çµŠé€ç·éé(F)
ïŒãã¬ããïŒãã€ãŒããªã€ã«ïŒ
èšç®åŒ
ïœïŒïŒŠïŒïœïŒWoïŒWtïŒ
ïŒïŒã»ïŒNoïŒNtïŒïŒïŒã»ïœïœïŒïŒ
ïŒ
ïŒWtâWoïŒWoïŒW2ïŒïŒã»ïŒïŒïœïŒïŒ
ïŒ
ïŒïŒ©ïŒïœïŒïŒ
ïŒ
ïŒïŒŠïŒïŒWtâWoïŒã»NtïŒNoïŒïŒ
è©Šéšåºã®ç·éééã®å¢éã¯å¯Ÿç
§åºïŒã«å¯ŸããŠ
28.1ïŒ
ã察ç
§åºïŒã«å¯ŸããŠã¯13ïŒ
倫ã
äžåããæ¬
çºæ溶液çµæç©ã¯æé·ä¿é²ã«å¯äžããããšãèªã
ãããã
䜿çšäŸ ïŒ
å®æœäŸïŒã§è£œé ãã溶液çµæç©ãå¡©é
žã§äžååŸ
察象éãããã€çšéãšããŠé£Œè²è©Šéšããã飌æã¯
ãã³èã®ããé€ãšãä»ã®ææãæ·»å æ··é¬ããŠçµŠé€
ãããåææã®é
åå²åã¯æ¬¡ã®ç¬¬ïŒè¡šã®éããšã
ãã[Table] In addition, the autolysis solution prepared under the condition-2 of Comparative Example-(b) above (non-protein nitrogen content/total nitrogen content =
0.15) was also applied to coho salmon breeding in the same way as in this usage example, but only results comparable to control group-2 were obtained. Number of rearing days (t) 36 days Total feeding weight (F) (pellets + feed oil) Calculation formula f=F/{(Wo+Wt) /2ã»(No+Nt)/2ã»t} (%) I=WtâWo/Wo+W 2 /2ã»1/t(%) E=I/f(%) R=F/(Wt-Wo)ã»Nt+No/2 Increase in total fish weight in test area compared to control area 1
28.1% and 13% higher than Control Group 2, respectively, which indicates that the solution composition of the present invention contributes to growth promotion. Use Example 2 After neutralizing the solution composition produced in Example 2 with hydrochloric acid, a breeding test was conducted using target fish as young red sea bream. The feed was shrimp paste and other ingredients were added and kneaded. The blending ratio of each material was as shown in Table 6 below.
ãè¡šã
ïŒåäœïŒéééšïŒ
飌è²ã¯æµ·é¢å°å²ç¶²çç°ïŒïŒÃïŒÃïŒïœïŒã« å
åŸ30æ¥ã®çšé1500å°ŸæŸé€ãã飌è²æ¥æ°35æ¥ã§è¡ã€
ããçµæã¯æ¬¡ã®ç¬¬ïŒè¡šã®éãã§è©Šéšåºã«ãããŠç¹
ã«åæã®æé€ã掻çºã§ãã€ãã[Table] (Unit: parts by weight)
Rearing was carried out for 35 days, with 1,500 juveniles released 30 days after hatching into small sea-surface net pens (2 x 2 x 2 m). The results are shown in Table 7 below, showing that feeding was particularly active in the early stages in the test plots.
ãè¡šã
䜿çšäŸ ïŒ
å®æœäŸïŒã§åŸããã液åã¹ãã«ãªã溶液çµæç©
ãå¡©é
žã§äžåããçé¯çšéã察象ã«æ¬¡ã®é£Œè²è©Šéš
ã«äŸãããåºç€é£Œæã¯é¯çšã¯ã©ã³ãã«ïŒåååïŒ
ããŸãšçšéçšïŒ¡ãæ¥æž
飌ææ ªåŒäŒç€Ÿè£œïŒãšãä»ã®
ææã墳é§åšã§å¢³é§ãããããµãŒäžã§æ¹æãããŠ
ããã¯ã©ã³ãã«ã«åäžã«æ·»å ãããåææã®é
å
å²åã¯æ¬¡ã®ç¬¬ïŒè¡šã®éãã§ãããæ°Žã飌æçšå±ç
å€ã液åã¹ãã«ãªã溶液çµæç©äžè
ãæ··åãã溶
液ãšããŠåæ£ã»æº¶è§£ãããŠçšããã[Table] Usage Example 3 The liquefied spirulina solution composition obtained in Example 3 was neutralized with hydrochloric acid and subjected to the following rearing test on juvenile red carp. The basic feed is crumble for carp (product name:
A for Yamato fry (manufactured by Nisshin Feed Co., Ltd.) and other ingredients were atomized using a munger and uniformly added to the crumble being stirred in a mixer. The blending ratio of each material is as shown in Table 8 below, and water, a feed spreader, and a liquefied spirulina solution composition were mixed and used as a solution that was dispersed and dissolved.
ãè¡šã
ïŒåäœïŒéééšïŒ
飌è²è©Šéšã¯çšé1000å°Ÿã30æ¥éïŒÃïŒïœã®æ°Žæ§œ
ã«å
¥ãå®æœãã第ïŒè¡šã«ç€ºãããšããã®çµæãåŸ
ãã
ãªãã絊飌çã¯åæïŒãïŒïŒ
ãç®å®ã«å¿
èŠã«å¿
ãå æžããäž¡åºãšãåéäžããç·çµŠé£Œéã¯ã¯ã©ã³
ãã«ãšããŠ3420ïœãšãªã€ãã
ãªããè©Šéšåºé£Œæã®èªå·±æ¶å液ã®äŸçµŠéã¯ä¹Ÿé
éæç®ã§ã¯ã©ã³ãã«ã«å¯ŸããŠçŽ0.02ïŒ
ã®ã¹ãã«ãª
ãã«çžåœããã[Table] (Unit: parts by weight)
A rearing test was carried out by placing 1000 fry in a 5 x 8 m aquarium for 30 days, and the results shown in Table 9 were obtained. The feeding rate was initially set at 6 to 7% and adjusted as necessary, and the same amount was fed to both groups, resulting in a total feeding amount of 3420 g as crumble. In addition, the amount of autolysed fluid supplied in the test group feed was equivalent to approximately 0.02% spirulina in the crumble on a dry weight basis.
第ïŒå³ã¯æ¬çºæ溶液çµæç©äžã«æº¶åããŠããæ
åã®çŽ«å€éšåžå
æ²ç·å³ã§ããã第ïŒå³ã¯æ¶ååŠç
æéã«å¯Ÿããåèšæåã®æ¥µå€§åžåæ³¢é·éšïŒ260n
ïœïŒã«ãããåžå
床ã®å€åã瀺ãæ²ç·å³ã§ããã
FIG. 1 is an ultraviolet absorption curve diagram of the components dissolved in the solution composition of the present invention, and FIG. 2 shows the maximum absorption wavelength region (260n
It is a curve diagram showing the change in absorbance in m).
Claims (1)
ããåæ£æº¶æ¶²ã®PHã8.0ã11.5ã®ç¯å²ã«ä¿æããª
ããã枩床ã25ã55âã«å¶åŸ¡ããŠåèšã¹ãã«ãªã
è»äœãèªå·±æ¶åããããåèšåæ£æº¶æ¶²ã®å šçªçŽ å«
éã«å¯Ÿããéèçœæ çªçŽ å«éãå°ããšã20ïŒ ã«ã
ã液åã¹ãã«ãªã溶液çµæç©ã ïŒ ã¹ãã«ãªãè»äœã®èªå·±æ¶å液ãå åŠçç¹æ§ã«
ãããŠ260nïœã«æ¥µå€§åžåã瀺ãç©è³ªãæããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ¶²åã¹ãã«ãªã溶液çµ
æç©ã ïŒ ã¹ãã«ãªãè»äœãã¹ãã«ãªãããã·ãããã³
ã¹ãã«ãªããã©ãã³ã·ã¹ã®äžæ¹åã¯äž¡è ã§ããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒåã¯ç¬¬ïŒé èšèŒã®æ¶²åã¹ãã«ãª
ã溶液çµæç©ã ïŒ ã¹ãã«ãªãçè»äœãã¢ã«ã«ãªæ§æ°Žæº¶æ¶²ã«åæ£
ããåæ£æº¶æ¶²ã®PHã8.0ã11.5ã®ç¯å²ã«ä¿æããª
ããã枩床ã25ã55âã«å¶åŸ¡ããŠåèšã¹ãã«ãªã
è»äœãèªå·±æ¶åããããåèšåæ£æº¶æ¶²ã®å šçªçŽ å«
éã«å¯Ÿããéèçœæ çªçŽ å«éãå°ããšã20ïŒ ã«å¢
倧ãã液åã¹ãã«ãªã溶液çµæç©ã®è£œé æ³ã ïŒ ã¹ãã«ãªãè»äœãã¹ãã«ãªãããã·ãããã³
ã¹ãã«ãªããã©ãã³ã·ã¹ã®äžæ¹åã¯äž¡è ã§ããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ¶²åã¹ãã«ãªã溶液çµ
æç©ã®è£œé æ³ã[Claims] 1. The Spirulina algae are self-digested by controlling the temperature at 25-55°C while maintaining the pH of a dispersion solution in which Spirulina live algae are dispersed in an alkaline aqueous solution in the range of 8.0-11.5. , a liquefied spirulina solution composition having a non-protein nitrogen content of at least 20% relative to the total nitrogen content of the dispersion solution. 2. The liquefied spirulina solution composition according to claim 1, wherein the autolyzed solution of spirulina algae contains a substance that exhibits maximum absorption at 260 nm in optical properties. 3. The liquefied Spirulina solution composition according to claim 1 or 2, wherein the Spirulina algae is one or both of Spirulina maxima and Spirulina platensis. 4 While maintaining the pH of the dispersion solution of Spirulina living algae dispersed in an alkaline aqueous solution in the range of 8.0 to 11.5, the temperature is controlled at 25 to 55°C to autolyze the Spirulina algae, and all of the dispersion solution is A method for producing a liquefied spirulina solution composition that increases the non-protein nitrogen content to nitrogen content by at least 20%. 5. The method for producing a liquefied Spirulina solution composition according to claim 4, wherein the Spirulina algae is one or both of Spirulina maxima and Spirulina platensis.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11108380A JPS5736981A (en) | 1980-08-14 | 1980-08-14 | Solution composition of liquefied spirulina and its preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11108380A JPS5736981A (en) | 1980-08-14 | 1980-08-14 | Solution composition of liquefied spirulina and its preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5736981A JPS5736981A (en) | 1982-02-27 |
JPS6350992B2 true JPS6350992B2 (en) | 1988-10-12 |
Family
ID=14551944
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11108380A Granted JPS5736981A (en) | 1980-08-14 | 1980-08-14 | Solution composition of liquefied spirulina and its preparation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5736981A (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4595505A (en) * | 1984-05-30 | 1986-06-17 | Solmat Systems, Ltd. | Method for suppressing algal growth in solar ponds |
US6537570B1 (en) | 1996-12-09 | 2003-03-25 | Kelvin Winston Duncan | Method of biological control |
KR100844189B1 (en) * | 2007-06-14 | 2008-07-04 | íêµìëª ê³µíì°êµ¬ì | [ ]Spirulina platensis M20CJK3[KCTC11127BP] characterized by enhanced floatation of its cell |
CN101869274A (en) * | 2010-05-17 | 2010-10-27 | å¹¿è¥¿å€§åŠ | Autolysis method of spiral seaweed tissue |
-
1980
- 1980-08-14 JP JP11108380A patent/JPS5736981A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5736981A (en) | 1982-02-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5459980B2 (en) | Method for producing health food, feed and fertilizer and complex fermented fungus for the production | |
JP2002238466A (en) | Feed additive | |
CN109479772B (en) | Artificial propagation technical method for rainbow trout | |
RU2345139C2 (en) | Method of processing synanthropic fly larva | |
CN104186431A (en) | High-density artemia breeding method with single-cell protein single-step food chain utilized | |
Silverman | In vitro cultivation procedures for parasitic helminths | |
Baburina et al. | Chemical and biotechnological processing of collagen-containing raw materials into functional components of feed suitable for production of high-quality meat from farm animals | |
JPS6350992B2 (en) | ||
RU2688470C1 (en) | Method of obtaining entomological biomass - raw material for production of fodder additives | |
SU674653A3 (en) | Feed for animals | |
CN1579196A (en) | Engineered fly biological high-protein powder | |
RU2388318C1 (en) | Method of fodder product preparation | |
CN106721586A (en) | It is a kind of to adjust metabolism and promote shrimp culture feather hydrolysis powder feed and preparation method thereof of body detoxification | |
RU2016510C1 (en) | Organomineral substrate showing biological stimulation property | |
RU2103360C1 (en) | Nutrient medium for culturing eucaryotic cells and method for preparing base of proteolytic hydrolyzate medium | |
JP2819513B2 (en) | Cultured seaweed growth promotion treatment method | |
CN105707441A (en) | Technology for producing selenium-enriched fly larva animal feed by using livestock dung | |
RU2133097C1 (en) | Method of preparing food addition "vitapeptid", food addition "vitapeptid" | |
RU2020153C1 (en) | Method of preparing of enzyme hydrolysate and nutrient medium for cultivation of eucaryotic cells | |
RU2068879C1 (en) | Method of preparing enzymatic hydrolyzate and nutrient medium for eucaryotic cells cultivation | |
JP3327943B2 (en) | Method for producing bioactivator | |
US3331356A (en) | Sterilization of fish in their aquatic environment to produce maximum size and weight per unit of water surface | |
JP2002199848A (en) | Formulated feed for aquatic invertebrate | |
CN114097711B (en) | Method for inducing yellow meal worm to produce antibacterial peptide, preparation method and application of yellow meal worm antibacterial peptide | |
SU718079A1 (en) | Feed protein production method |