JPS6332080B2 - - Google Patents
Info
- Publication number
- JPS6332080B2 JPS6332080B2 JP54150111A JP15011179A JPS6332080B2 JP S6332080 B2 JPS6332080 B2 JP S6332080B2 JP 54150111 A JP54150111 A JP 54150111A JP 15011179 A JP15011179 A JP 15011179A JP S6332080 B2 JPS6332080 B2 JP S6332080B2
- Authority
- JP
- Japan
- Prior art keywords
- antibiotic
- strain
- formula represents
- formula
- anthracycline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000003115 biocidal effect Effects 0.000 claims description 43
- 239000003242 anti bacterial agent Substances 0.000 claims description 21
- 229940088710 antibiotic agent Drugs 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 15
- 229910052721 tungsten Inorganic materials 0.000 claims description 14
- 229910052727 yttrium Inorganic materials 0.000 claims description 14
- 239000003817 anthracycline antibiotic agent Substances 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 10
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 claims description 9
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 claims description 9
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 claims description 9
- 235000013372 meat Nutrition 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 241000187747 Streptomyces Species 0.000 claims description 8
- 229940045799 anthracyclines and related substance Drugs 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 7
- 229930182470 glycoside Natural products 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 150000002338 glycosides Chemical class 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 229950001725 carubicin Drugs 0.000 claims description 3
- 238000002703 mutagenesis Methods 0.000 claims description 3
- 231100000350 mutagenesis Toxicity 0.000 claims description 3
- 238000011160 research Methods 0.000 claims description 3
- 238000010898 silica gel chromatography Methods 0.000 claims description 2
- 239000012736 aqueous medium Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 229910017053 inorganic salt Inorganic materials 0.000 claims 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 24
- 238000000855 fermentation Methods 0.000 description 18
- 230000004151 fermentation Effects 0.000 description 18
- 239000000203 mixture Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 241000187081 Streptomyces peucetius Species 0.000 description 6
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 description 6
- 238000002844 melting Methods 0.000 description 6
- 230000008018 melting Effects 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- WPJRFCZKZXBUNI-HCWXCVPCSA-N daunosamine Chemical compound C[C@H](O)[C@@H](O)[C@@H](N)CC=O WPJRFCZKZXBUNI-HCWXCVPCSA-N 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 150000002337 glycosamines Chemical class 0.000 description 5
- -1 hydrochloric Chemical class 0.000 description 5
- 150000003840 hydrochlorides Chemical class 0.000 description 5
- WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229940009456 adriamycin Drugs 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 235000001727 glucose Nutrition 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000000704 physical effect Effects 0.000 description 4
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 4
- 229940124530 sulfonamide Drugs 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000004816 paper chromatography Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 150000003863 ammonium salts Chemical class 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 229910000365 copper sulfate Inorganic materials 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000012458 free base Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000012074 organic phase Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- 229960001763 zinc sulfate Drugs 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- 241000180579 Arca Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- WNBCMONIPIJTSB-BGNCJLHMSA-N Cichoriin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1c(O)cc2c(OC(=O)C=C2)c1 WNBCMONIPIJTSB-BGNCJLHMSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 239000005696 Diammonium phosphate Substances 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 1
- GGLZPLKKBSSKCX-YFKPBYRVSA-N L-ethionine Chemical compound CCSCC[C@H](N)C(O)=O GGLZPLKKBSSKCX-YFKPBYRVSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 1
- UIHCLUNTQKBZGK-UHFFFAOYSA-N Methyl isobutyl ketone Natural products CCC(C)C(C)=O UIHCLUNTQKBZGK-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 241000576755 Sclerotia Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- IUJDSEJGGMCXSG-UHFFFAOYSA-N Thiopental Chemical compound CCCC(C)C1(CC)C(=O)NC(=S)NC1=O IUJDSEJGGMCXSG-UHFFFAOYSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000011260 aqueous acid Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000012474 bioautography Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 235000010633 broth Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000012045 crude solution Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 229960000975 daunorubicin Drugs 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- YWEUIGNSBFLMFL-UHFFFAOYSA-N diphosphonate Chemical compound O=P(=O)OP(=O)=O YWEUIGNSBFLMFL-UHFFFAOYSA-N 0.000 description 1
- 229960004679 doxorubicin Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 1
- 229940093496 esculin Drugs 0.000 description 1
- AWRMZKLXZLNBBK-UHFFFAOYSA-N esculin Natural products OC1OC(COc2cc3C=CC(=O)Oc3cc2O)C(O)C(O)C1O AWRMZKLXZLNBBK-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000012869 germination medium Substances 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 235000011007 phosphoric acid Nutrition 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- DLYUQMMRRRQYAE-UHFFFAOYSA-N phosphorus pentoxide Inorganic materials O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012746 preparative thin layer chromatography Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000011158 quantitative evaluation Methods 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- VLYWMPOKSSWJAL-UHFFFAOYSA-N sulfamethoxypyridazine Chemical compound N1=NC(OC)=CC=C1NS(=O)(=O)C1=CC=C(N)C=C1 VLYWMPOKSSWJAL-UHFFFAOYSA-N 0.000 description 1
- 229960004936 sulfamethoxypyridazine Drugs 0.000 description 1
- 229960001544 sulfathiazole Drugs 0.000 description 1
- JNMRHUJNCSQMMB-UHFFFAOYSA-N sulfathiazole Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CS1 JNMRHUJNCSQMMB-UHFFFAOYSA-N 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002211 ultraviolet spectrum Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003021 water soluble solvent Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/252—Naphthacene radicals, e.g. daunomycins, adriamycins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Description
ãçºæã®è©³çŽ°ãªèª¬æã
æ¬çºæã¯ãæ°èŠã®ã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©
質ãéé µæ³ã«ãããã®è£œé ãç²è£œæº¶æ¶²ããã®ãã
ãæçç©è³ªã®åååã³æ¿çž®æ³ããã®ç²Ÿè£œæ³åã³ã
ã®å¡©ã®è£œæ³ã«é¢ããã æ¬çºæã«ã¯ãçšé圢ã§ãæ¿çž®ç©ãšããŠããã€çŽ
ç²çµæ¶åœ¢ã®æ°èŠæçç©è³ªãå å«ãããããããæ°
èŠã®ã¢ã³ãã©ãµã€ã¯ãªã³é¡ã¯æè «çå€ãšããŠãæ
èå€ãšããŠãæå¹ã§ããããã®æ°èŠæçç©è³ªã®äœ
çšå¹æã¯ããããã®ååŠçåã³ç©ççç¹æ§ãšå ±
ã«ãåŸæ¥å ¬ç¥ã®æçç©è³ªãšã¯ç°ãªãã æ°èŠæçç©è³ªïŒããã§ã¯æçç©è³ªïŒ·ãåã³ïŒº
ãšç§°ããïŒã¯ãã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€
ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠã¹ïŒStreptomyces
peucetius var.caeciusïŒã®å€ç°èæ ªå³ã¡ã¹ãã¬
ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ãªãŠã¬
ãŠã¹ïŒStreptomyces peucetius var.aureusïŒã®
éé µã®éã«åœ¢æãããã æ¬çºæã®æ¹æ³ã§äœ¿çšããã埮çç©ã¯ãã¹ãã¬ã
ããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠ
ã¹ãâã¡ãã«âNâ²âãããââããããœã°
ã¢ããžã³ã§ã®å€ç°èªçºåŠçã«ãã€ãŠåŸããã
ïŒARCA MONEçã«ããBiotechnol.Bioengeen
XIå·»ã1969幎ã1101ã1110é åç §ïŒãããããŠåŸ
ãæ°èŠèæ ªã¯ãã¢ã«ãã¿ãªã¢ã»ã³ã¬ã¯ã·ãšã³ã»ãª
ãã»ãã€ã¯ããªã«ã¬ãã¹ã ã¹ïŒFarmitalia
Collection of MicroorganismsïŒã®ã³ãŒãçªå·
416F.I.ãæãããã€ãããšã»ã¶ã³ã ã«ã³ã°ã»ã
ã¯ããªã«ã¬ãã¹ã¡ã³ïŒDeutsche Sammlung
MikroorganismenïŒã«å¯èšãããŠãçªå·
DSM1367ãæããã¢ã¡ãªã«ã³ã»ã¿ã€ãã»ã«ã«ã
ãŠã¢ã»ã³ã¬ã¯ã·ãšã³ïŒAmerican Type Culture
CollectionïŒã§ATCC31428ãæãã埮工ç èå¯
第4622å·ãæããã å€ãã®æçç©è³ªåœ¢æå¹é€ã®å Žåã«ããããã
ã«ãèæ ª416F.I.ã®éé µã®éã«ãæåã®æ··åç©å
ã¯è€åç©ã圢æããããïŒçš®ã®çç©ã«äœçšããã¢
ã³ãã©ãµã€ã¯ãªã³æåïŒããã§ã¯æçç©è³ªïŒ·ã
åã³ïŒºãšç§°ããïŒã¯ãåèšåŸ®çç©ã«ããçæãã
ãè€åäœããåé¢ãããŠããã æçç©è³ªïŒ·ããåã³ïŒºã¯æ¬¡ã®æ§é åŒãæãã
ããšã確èªãããïŒ ïŒæçç©è³ªïŒ· ïŒâCoâCH2âOH ïŒæçç©è³ªïŒ¹ ïŒâCoâCH3 ïŒæçç©è³ªïŒº ïŒâCH2âCH3 ããã§ãããããæçç©è³ªïŒ·()ã¯11âããªã
ã·â14âããããã·âã«ã«ãããã€ã·ã³ã«ãæç
ç©è³ªïŒ¹()ã¯11âããªãã·âã«ã«ãããã€ã·ã³
ã«ãæçç©è³ªïŒº()ã¯11âããªãã·â13âããªã
ãœâã«ã«ãããã€ã·ã³ã«ãçžåœãããåèšã®ãã
ã«ããã¹ãŠã®åèšæåã¯ãããŠããµãã³ïŒïŒâã¢
ããâïŒïŒïŒïŒïŒâããªããªãã·ââãªããœâ
ãããœãŒã¹ïŒå³ã¡ããŠããã€ã·ã³åã³ã¢ããªã¢ã
ã€ã·ã³ã®ã¢ããç³æåãå«æããã¢ã³ãã©ãµã€ã¯
ãªã³ã°ãªã³ã·ãã§ããïŒF.ArcamoneãG.
CassinelliãP.OrezziãG.Franceschiåã³R.
Mondelliã«ãããJ.Am.Chem.Soc.86å·»5335é ã
1964幎ãåã³ç±³åœç¹èš±ç¬¬3590028å·æ现æžåç §ïŒã
ãã®æåã®æ§é ã¯èµ€å€ç·ã玫å€ç·ãå¯èŠç·ã質é
ã¹ãã¯ãã«åã³æ žç£æ°å ±é³Žã¹ãã¯ãã«åæã«ãã
枬å®ãããåŸã«èšèŒã®ååŠçåã³ç©ççããŒã¿ã§
åå®ãããã æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³è€åç©åã³ãã®åã ã®
æåã¯ãé žãšãå¡©åºãšãå¡©ã圢æãããã®è€åç©
åã³æåã®è¬ç©åŠçã«èªå®¹æ§ã®å¡©ã¯ãæ¬çºæã®ç¯
å²ã«å å«ãããããã®ãããªè¬ç©åŠçã«èªå®¹æ§ã®
å¡©ã®äŸã«ã¯é žäŸãã°å¡©é žãç¡«é žãç¡é žåã³çé žãš
ã®å¡©åã³éå±éœã€ãªã³äŸãã°ã¢ã«ã«ãªéå±åã¯ã¢
ã«ã«ãªåé¡éå±éœã€ãªã³äŸãã°ãããªãŠã ãã«ãª
ãŠã ãã«ã«ã·ãŠã åã³ãã°ãã·ãŠã åã³ä»ã®éœã€
ãªã³äŸãã°ïŒäŸ¡ã®ééœã€ãªã³ãšã®å¡©ãå å«ãã
ããæ¬çºæã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ª
ã®è£œé ãåŸã«èšèŒããã 416F.I.æ ªã®é¡åŸ®é¡ç¹æ§ åºè³ªãã»ã«ã¯ãçš®ã ã®é·ãã§çŽåŸ0.5ã0.9ÎŒã®
å šäœçã«åæããè糞ã§åœ¢æãããŠãããåè ã
ãç«ã€ãŠããæ°è糞ã¯ãçŽåŸ1.1ã1.6ÎŒã®å šäœç
ã«é·ãçŽç·ç¶ãžå±æããè糞ã§åœ¢æãããŠããã
ãããããè ºç¶æã§äž¡è¶³çµåå¥æ§ã®åæããŠãè
åã¯å€åæ§ã®é·ãã®é¢ããŠæ«ç«¯ã§ã«ã®åã¯ã«ãŒã
ã«ãªã€ãŠããè糞ãæãããèåã¯ç圢ã§æ¬¡ã®å¯ž
æ³ãæããïŒçŽåŸïŒã2ÎŒããŸãé£éé 眮ãããŠ
ããã次ãã§è§£æŸãããŠãããé»åé¡åŸ®é¡äžã§ã
ãã®èåã¯äžèŠåãªèŒªéã®ã»ãŒç圢ã§ç£ç¶è¡šé¢ã
æããã 416F.I.æ ªã®èçŒçç¹æ§ 416F.I.æ ªã®å¹é€ç¹æ§ã第ïŒè¡šã«ç€ºããçé·ã¯
ææ©å¹å°äžã§ãåæå¹å°äžã§ãäžè¬ã«è¯å¥œã§ã
ãã第ïŒè¡šã«èšèŒã®å¹å°äžã®çé·èŠ³å¯ã¯ã28âã§
ææž©ä¿æ第ïŒæ¥ç®ãããçé·åæ¢ãŸã§è¡ãªã€ãã
416F.I.æ ªã®çååŠçåã³ççåŠçç¹æ§ã第ïŒè¡š
ã«ç€ºãã 40âããé«ã枩床ã§ã¯çé·ã¯èªããããªãã第
ïŒè¡šã«èšèŒã®å¹å°äžã§ã¯èæ žåœ¢æã¯èªããããªã
ã€ãã ãè¡šã ã¯ãã¯ã¹ãã³ïŒWaksmanïŒS.A.ïŒãŒã»ã¢ã¯ã
ããã€ã»ãã¹ãThe ActinomycetesïŒå·»
ïŒ1961幎ïŒWilliam Wilkins Co.Baltimoreã ããªãã ïŒPridhamïŒT.G.ãã¢ã³ããŒãœã³
ïŒAndersonïŒP.ããªã¬ã€ïŒFoleyïŒïŒ£ããªã³ãã³
ããšã«ãµãŒïŒLindenfelserïŒL.A.ããã»ã«ãã€ã³
ïŒHesseltineïŒC.W.åã³ãããã€ã¯ã
ïŒBenedictïŒR.G.ïŒã¢ã³ããŒã¬ã³ïŒAnn.ïŒ1956ïŒ
1957幎947ã953é ã®ã¢ã»ã»ã¬ã¯ã·ãšã³ã»ãªãã»ã¡
ãã€ã¢ã»ããªã¢ã»ã¡ã€ã³ãã€ãã³ã¹ã»ã¢ã³ãã»ã
ããœãããã¯ã»ã¹ã¿ãã€ã¹ã»ãªãã»ã¹ãã¬ããã
ã€ã»ã¹ã»ã¢ã³ãããªãã€ã¯ã¹ïŒïŒ¡ selection of
media for maintenance and toxonomic
studjes of Streptomyces AntibioticsïŒç¬¬ïŒè¡š 416F.I.ïŒæ ªã®ççåŠçåã³çååŠçæ§
質 ã°ã«ã³ãŒã¹å©çšæ§ ïŒ ãµãã«ããŒã¹ã â âãã·ããŒã¹ ã ïŒ ãã³ãããŒã« ã â ïœâã€ãã·ããŒã« ã ïŒ ïŒ¬âã¢ã©ãããŒã¹ ã ïŒ ïŒ€âãã©ã¯ããŒã¹ ã ïŒ ã¢ããããŒã« ã â ã©ã¯ããŒã¹ ã â ïœ(+)ãã³ããŒã¹ ã ïŒ ãã«ããŒã¹ ã ïŒ ã©ãã€ããŒã¹ ã â âã©ã ããŒã¹ ã â αâαâãã¬ãããŒã¹ ã ïŒ ãšã¹ã¯ãªã³ ã ïŒ ã°ãªã»ããŒã« ã ïŒ ã¯ãšã³é žãããªãŠã ã ïŒ ã³ãã¯é žã¢ã³ã¢ã㢠ã ïŒ é ¢é žãããªãŠã ã â é ç³é žã¢ã³ã¢ã㢠ã â ã°ãªã³ãŒã²ã³ ã ïŒ ãã©ãã€ã³ ã â ãã€ãã¹é¢ã®æ€æ»ïŒ ãŒã©ãã³ã®æ¶²å ïŒ ããã·ã³å解 ïŒ ã¡ã©ãã³åœ¢æ â ãã³ãã³ã®å æ°Žå解 ïŒ H2S圢æ ïŒ ç¡é žå¡©éå ïŒ ãã«ã¯ïŒãããã³ååã³ååºïŒ ïŒ çç£æçç©è³ªïŒæ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ ïŒïŒéœæ§åå¿ âïŒé°æ§åå¿ ïŒ äœ¿çšããçåæ°ŽçŽ å©çšæ§è©Šéšçšå¹å°ã¯ãR.
D.ãŽã«ãã³ïŒGordonïŒåã³M.L.ã¹ãã¹
ïŒSmithïŒã«ããJ.ãã¯ããªãªããžã€
ïŒBacteriologyïŒ69å·»ïŒ1955幎ïŒ147ã150é ã«
èšèŒãããŠããã ïŒ ä»ã®ççåŠçåå¿çšå¹å°ã¯ãS.A.ã¯ã¯ã¹ãã³
ïŒWaksmanïŒã«ãããããŒã»ã¢ã¯ãããã€ã»ã
ã¹ãå·»ïŒ1961幎ïŒïŒThe Williams Wilkins
Companyã»BaltimoreïŒã«å ±åãããŠããã
ã®ã§ããã 416F.I.æ ªã®åå®åã³åé¡ 416F.I.æ ªã«ãã瀺ãããå šäœçç¹æ§ã¯ãæã
ãã«ãã¹ãã¬ãããã€ã»ã¹å±ã®èã«é¢ããŠã¯ã¯ã¹
ãã³åã³ãã³ãªãïŒHenriciïŒã«ãã瀺ãããŠã
ãç¹æ§ã«çžåœãããæŽã«ã416F.I.æ ªã§ç€ºããã
圢æ åŠçãå¹é€äžåã³ççåŠçç¹æ§ã¯ãã¹ãã¬ã
ããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠ
ã¹ïŒStreptomyces peucetius var.caesiusïŒã«é¢
ããç¹æ§ïŒç±³åœç¹èš±ç¬¬3590028å·æ现æžïŒ
Arcamoneçã«ããBiotechnol.BioengeenXI
ïŒ1969幎ïŒ1101ã1110é åç §ïŒã«çžåœããããã
ãããšã¯ããã®é»è²å¯æº¶æ§è²çŽ ã®åœ¢æèœã«åºã¥
ããïœâã€ãã·ããŒã«ãâã¢ã©ãããŒã¹åã³ãš
ã¹ã¯ãªã³ãå©çšãããããµãã«ããŒã¹ããã³ãã
ãŒã«åã³ã©ãã€ããŒã¹ãå©çšããªãããšã«åºã¥
ãããã€æçµçã«ãæ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æ
çç©è³ªãçç£ããããšã«åºã¥ããç°ãªãã åŸã€ãŠã416F.I.æ ªã¯ãã¹ãã¬ãããã€ã»ã¹ã»
ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠã¹ã®å€çš®ã§ã
ã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»
ãªãŠã¬ãŠã¹ïŒStreptomyces peucetius var.
aureusïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒãšåœåããã éé µæ³ ãã®æ¹æ³ã¯ãæ £çšã®å ¬ç¥æ¹æ³ã§å®æœãããçªç¶
å€ç°ãã埮çç©ããäºãæ» èãã液äœå¹å°äžã§å¥œ
æ°æ§æ¡ä»¶äžã«25âã37âïŒæå©ã«28âïŒã®æž©åºŠã§
ïŒãïŒæ¥ïŒæå©ã«ïŒæ¥ïŒéãåœå6.5ã7.0ã§ãé
é µã®çµæã«ã¯6.5ã8.0ã®PHå€ã§éé µãããããšã
ããªãã å¹å°ã¯ççŽ åã³çªçŽ æºäžŠã³ã«ç¡æ©å¡©ãããªãã ççŽ æºã¯ãäŸãã°ããã³ãã³ãããã¹ããªã³ã
ã°ã«ã³ãŒã¹ãã°ãªã»ãªã³ããã³ãããããã«ããŒ
ã¹ãã³ãŒã³ã»ã¹ãã€ãŒãã»ãªã«ãŒãå¯æº¶æ§èžæº
ç©ã倧è±æ²¹åã¯å€§è±ç²ã§ãã€ãŠãããçªçŽ æºã¯ã
çªçŽ ãå«æããåèšã®ççŽ æºä»¥å€ã«ãäŸãã°ä¹Ÿç¥
é µæ¯ãããŒããããã³åã¯ã«ãŒã€ã³ã§ãã€ãŠã
ããè¯å¥œãªçµæã¯ãã¢ã³ã¢ããŠã å¡©äŸãã°ç¡é žã¢
ã³ã¢ããŠã ãç¡«é žã¢ã³ã¢ããŠã åã³çé žäºã¢ã³ã¢
ããŠã ãçšããããšã«ãã€ãŠãåŸãããããã®ç
ç£ã«ãšã€ãŠæçšãªç¡æ©å¡©ã¯äœ¿çšå¹å°ã«äŸã€ãŠå€ã€
ãŠãããè€åç©è³ªäŸãã°çš®ã ãªç©ç²åã³å¹é€æ®æž£
ãå«æããå¹å°äžã«ãçé žã«ã«ã·ãŠã åã³çé žã
ããªãŠã åã¯çé žã«ãªãŠã ãæ·»å ããããšã¯æçš
ã§ããããšãç«èšŒããããã°ã«ã³ãŒã¹åã¯ã¢ã³ã¢
ããŠã å¡©ãå«æããå¹å°äžã«ã¯ãããé«æ¿åºŠã®ã«
ãªãŠã ããããªãŠã åã¯ã«ã«ã·ãŠã ã®ç¡æ©å¡©ãå¿
èŠã§ãããæŽã«æ¥µå°éã®éå±äŸãã°éãäºéã
é ããã°ãã·ãŠã åã³ãã³ã¬ã³ã®å¡©ãå¿ èŠã§ã
ããã¹ã«ãã¢ãã«ã¢ãããã¹ã«ãã¢ãã¢ãŸãŒã«ã
ã¹ã«ãã¢ããªããžã³ããã³ããªãã«ãã¿ãŒã«åã³
ãšããªãã³ãªã©ã®ååç©ãå«æããç¡«é»ãæ·»å ã
ãããšãæçšã§ããã éé µã¯ãšãŒã¬ã³ãã€ã€ãŒãã©ã¹ã³äžåã¯çš®ã ã®
容éã®å®éšçšåã¯å·¥æ¥çšéé µåšäžã§å®æœã§ããã åææ³ éé µèæ±åã³ç²è£œèª¿åç©ã®è©Šæãã1/15ïŒçé ž
å¡©ç·©è¡æ¶²ã§ãPH5.4ã§ç·©è¡ãããã¯ãããã³
ïŒWhatmanïŒNo.ïŒããŒããŒãçšããå±éå€ãšããŠ
ïœâãããããŒã«å¯Ÿé ¢é žãšãã«å¯Ÿæ°ŽïŒïŒïŒïŒïŒ
ïŒïŒã®æ··åç©ãçšããŠããŒããŒã¯ãããã°ã©ãã€
ã«ããããã®ããŒããŒçããã·ã«ã¹ã»ã¹ãããªã¹
ïŒBacillus subtilisïŒã«å¯ŸããŠããªãªãŒãã°ã©ã
ã€ã«ããããšãïŒçš®ã®äž»èŠæåãçŸããããšãèª
ããããããããæçç©è³ªïŒ·ïŒRf0.45ïŒãïŒRf
0.64ïŒåã³ïŒºïŒRf0.70ïŒãšåœåãããïŒçš®ã®å°é
æåãçŸããããããã¯ã°ãªã³ã·ãåã³ïŒ£ïŒã
ãããRf0.30åã³0.55ïŒãšåå®ãããç¹é¡æ54â
12850å·æ现æžã«èšèŒãããŠããã éé µèæ±äžã«ååšããå šäœçãªé»è²æåã®éç
è©äŸ¡ã¯ã次ã®æ¹æ³ã§è¡ãªãããšãã§ãããèæ±
ïŒPH8.6ã«èª¿æŽããïŒïŒè©Šæã«ãïŒåéã®ã¯ããã
ã«ã ïŒã¡ã¿ããŒã«ïŒïŒïŒïŒïŒãå ããçããæ··å
ç©ã宀枩ã§ïŒåéé³æ³¢åŠçããã次ãã§ãææ©çž
ã®è©ŠæïŒé žæ§ã¡ã¿ããŒã«ã§çšéïŒã§ãé»è²ã®ã¢ã³
ãã©ãµã€ã¯ãªã³åã³ãã®ã¢ã°ãªã³ã³ã®å šå«éã
430nmã§åå å 床枬å®ããããšãã§ãããæžå§äž
ã«æ¿çž®ãããææ©çžã®è©Šæã§ãåäžã®æçç©è³ªã®
éç枬å®ã¯ãåèšç³»ãçšãã調æŽçšèå±€ã¯ããã
ã°ã©ãã€ã§åŸãããšãã§ãããçš®ã ç°ãªãé»è²åž¯
åãæŠãåããã¡ã¿ããŒã«ã§å±éããããåæå
ã430nmã§åå å 床枬å®ãããæçç©è³ªïŒ¹ã¯ãé
äŸéé µèæ±äžã®äž»èŠæåã§ããã åé¢æ³ éé µãçµäºããåŸãæå¹æåã¯è糞äžåã³éé µ
液äžã«å«æãããŠããããã®ã¢ã³ãã©ãµã€ã¯ãªã³
ç³»æçç©è³ªã¯ãPH8.5ã9.0ã§éé¢ã®å¡©åºãšããŠå¹
é€æ¶²ããå šäœãšããŠãæ°Žãšæ··ãããªãææ©æº¶å€äŸ
ãã°ããã¿ããŒã«ãã¡ãã«ã€ãœããã«ã±ãã³ãã¯
ãããã«ã ãäºå¡©åã¡ãã¬ã³åã³é ¢é žãšãã«ã§æœ
åºããããšãã§ãããè糞åã³éé µæ¶²ãPHïŒã§çª
è»åãçšããéã«ããåé¢ãã次ãã§å¥ã ã«æœ
åºããã®ãæå©ã§ããã æ»ã氎溶æ§æº¶å€äŸãã°ã¢ã»ãã³åã³äœçŽã¢ã«
ã³ãŒã«æå©ã«ã¡ã¿ããŒã«ã§æœåºããã è糞æœåºç©ãéããæžå§äžã«æ¿çž®ããã æ¿çž®ç©ãéããèæ±ãšå ±ã«éããPH8.5ãïŒ
ã«èª¿ç¯ãã次ãã§ãæ°Žãšæ··ããããªãææ©æº¶å€ã
æå©ã«ã¯ãããã«ã åã¯ïœâãã¿ããŒã«ã§æœåºã
ããæœåºæ¶²ãæžå§äžã«æ¿çž®ãããã®ã¢ã³ãã©ãµã€
ã¯ãªã³ç³»æçç©è³ªã¯ïŒåéã®ïœâãããµã³ã®æ·»å
ã«ããæ²æ®¿ãããããšãã§ãããç²è£œæ··åç©ã®æ
åããã«ã©ã ã¯ãããã°ã©ãã€æ³ã§åå¥ã粟補ã
ãããšãã§ããã ç²Ÿè£œæ³ æçç©è³ªæŽ»æ§ãæŽã«ç²Ÿè£œãããã€ãããïŒæå
å³ã¡æçç©è³ªïŒ·ãåã³ïŒºã«åããããšã¯ãã·ãª
ã«ã²ã«ã«ã©ã ã¯ãããã°ã©ãã€ã«ããè¡ãªãããš
ãã§ãããç²è£œã®æ©è€è²ç²æ«ãã¯ãããã«ã äžã«
溶ããã溶液ããã¯ãããã«ã ïŒã¡ã¿ããŒã«ïŒæ°Ž
ã®åŸé ãçšããŠãç·©è¡ã·ãªã«ã²ã«äžã®ã¯ãããã°
ã©ãã€ã«ãããããŸãæçç©è³ªïŒºãã94.8ïŒïŒïŒ
0.2âæ··åç©ã§æº¶é¢ãããåŒç¶ã92.2ïŒ7.5ïŒ0.3â
æ··åç©ã§æçç©è³ªïŒ¹ãã次ãã§300ïŒ55ïŒïŒâæ··
åç©ã§æçç©è³ªïŒ·ã溶é¢ãããããããã®æå
ã¯ãéäŸããŒããŒã¯ãããã°ã©ãã€åã³èå±€ã¯ã
ããã°ã©ãã€ã§ç€ºãããããã«åé¢ãããæçç©
質ãåã³ïŒºã¯ãåœéã®ã¡ã¿ããŒã«æ§å¡©é žã®æ·»
å ã®éã«ãããã®å¡©é žå¡©ãšããŠãçµæ¶ã§åŸãã
ãã ååŠçåã³ç©ççç¹æ§ æçç©è³ªïŒ·ãåã³ïŒºã¯ãå ¬ç¥ã®ã¢ã³ãã©ãµã€
ã¯ãªã³ç³»æçç©è³ªã«å ±éã®ããã€ãã®ç¹æ§ã瀺ã
ãããããã¯ããã®ååŠçåã³ç©ççç¹æ§ã«åºã¥
ãåºå¥ã§ããã ããããã¹ãŠã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ã¯åæ§
ãªæº¶è§£æ§ãæããå³ã¡éé¢å¡©åºãšããŠãããã¯ã
ã¯ãããã«ã ãäºå¡©åã¡ãã¬ã³ãã¢ã»ãã³ãã¡ã¿
ããŒã«ããšã¿ããŒã«ãå«æ°Žã¢ã«ã³ãŒã«ãé žæ§æ°Žã
ãžãªããµã³åã³ããªãžã³äžã«æº¶ããããžãšãã«ãš
ãŒãã«ãïœâãããµã³ãã·ã¯ããããµã³åã³ç³æ²¹
ãšãŒãã«äžã«ã¯å ãã«å¯æº¶ã§ãããåã¯äžæº¶ã§ã
ããå¡©é žå¡©ãšããŠããããã¯æ°Žãã¡ã¿ããŒã«ããš
ã¿ããŒã«åã³å«æ°Žã¢ã«ã³ãŒã«äžã«æº¶ããã¢ã»ã
ã³ããã³ãŒã³ãã¯ãããã«ã ããžãšãã«ãšãŒãã«
åã³ç³æ²¹ãšãŒãã«äžã«ã¯äžæº¶ã§ããããããæ°èŠ
ååç©ã¯ãäžæ§åã³é žæ§æº¶æ¶²äžã§æ©é»è²ã®æ瀺è¬
ãšããŠäœ¿çšã§ãããã®æº¶æ¶²äžã§çŽ«å€ç·äžã§ã¯åž¯ç·
é»è²è¢å ãã瀺ãããã®ã¢ã«ã«ãªæº¶æ¶²ã¯èµ€è€è²ã§
ãããã¢ã«ã³ãŒã«æ§é ¢é žãã°ãã·ãŠã ã§åŠçãã
éã«ã¯æ©èµ€è²æº¶æ¶²ãçããããããã®ãã¹ãŠã®ç¹
æ§åã³çŽ«å€ç·åã³å¯èŠå ç·ã«ãããåžåã¹ãã¯ã
ã«ã¯ããããååç©ãã¢ã³ãã©ãµã€ã¯ãªã³ç³»æç
ç©è³ªã®çŸ€ã«å±ããããšã瀺ããŠããã ïŒåã®äž»æåå³ã¡å¡©é žå¡©ãšããŠåé¢ãããæç
ç©è³ªïŒ·ãåã³ïŒºã®ååŠçåã³ç©ççç¹æ§ã第ïŒ
è¡šã«ç€ºãã æçç©è³ªïŒ·ãåã³ïŒºã¯ãããã©ãµã€ã¯ãªã³ç³»
ã¢ã°ãªã³ã³ïŒã¢ã³ãã©ãµã€ã¯ãªãã³ïŒãšã¢ããç³
ã«ãã圢æãããã°ãªã³ã·ãååç©ã§ãããäŸã
ã°æçç©è³ªïŒ¹ã0.2Nå¡©é žãçšã20åé90âã§é ž
æ§å æ°Žå解ãããšãæ°Žã«äžæº¶ã®ã¢ã°ãªã³ã³ãé»è²
æ©è²éç¶ã§åŸããããèç¹168âãUVã¹ãã¯ã
ã«Î»MeOH nax227ã258ã430nmïŒE1%1cnïŒ880ã560ã
263ïŒïŒ I.R.ã¹ãã¯ãã«ïŒKBrïŒïŒ3430ã2920ã 1710ã1670ã1620ã1470ã 1450ã1420ã1385ã1355ã 1285ã1260ã1210ã1185ã 1160ã1125ã1085ã1040ã 1015ã982ã930ã900ã885ã 860åã³835cm-1. æçç©è³ªïŒ¹ã®ã¢ã°ãªã³ã³ã¯C20H16O7ã«çžåœã
ãå®éšåŒãæããïŒååé368.33ãååéã¯ã質
éã¹ãã¯ãã«ã§ç¢ºèªããïŒïœïŒe368ïŒM+ïŒïŒ332
ïŒïŒâïŒ H2OïŒïŒ317ïŒïŒâïŒ H2OâCH3ïŒåã³
289ïŒïŒâïŒ H2OâCOCH3ïŒ ä»ã®ç²Ÿè£œæçç©è³ªã®é žæ§å æ°Žå解ã«ãããïŒçš®
ã®çš®ã ã®é»è²ã¢ã°ãªã³ã³ãçãã第ïŒè¡šã«èšèŒã®
ãããªã¯ãããã°ã©ãã€ç¹æ§ã瀺ããåå®ãã
ãã 粟補æçç©è³ªïŒ·ãåã³ïŒºã®ãã¹ãŠã®é žæ§å æ°Ž
å解ã®æ°Žæ§å¯æº¶æ§ãã©ã¯ã·ãšã³ã¯ãããŠããµãã³
ïŒïŒâã¢ããâïŒïŒïŒïŒïŒâããªããªãã·ââ
ãªããœãããœãŒã¹ïŒãšåãã¯ãããã°ã©ãã€ç¹æ§
ãæããéå æ§ã¢ããç³ãå³ã¡ããŠããã€ã·ã³å
ã³ã¢ããªã¢ãã€ã·ã³ã®ã¢ããç³æåãå«æããã
ããã§ããŠããã€ã·ã³åã³ã¢ããªã¢ãã€ã·ã³ïŒã
ããã¯ããããåååã§ããïŒãšã¯ãããããã
ãŠãã«ãã·ã³åã³ãããœã«ãã·ã³ãæå³ããŠçšã
ãããŠããã ãè¡šã ãè¡šã ãè¡šã çç©åŠç掻æ§ããŒã¿ (a) æèäœçš æšæºè©Šéšç®¡çšéæ³ãçšããŠããã€ãã®åŸ®çç©
ã«å¯ŸããŠæž¬å®ããæçç©è³ªïŒ·ãåã³ïŒºïŒå¡©é ž
å¡©ãšããŠïŒã®è©Šéšç®¡å æå°æå¶æ¿åºŠïŒMICïŒ
ã第ïŒè¡šã«ç€ºãã ãè¡šã ãè¡šã (b) æè «çäœçš æ°èŠæçç©è³ªïŒ·ãåã³ïŒºããã©çŽ°è
ïŒHela cellsïŒã«å¯ŸããŠãè©Šéšç®¡å ã§è©Šéšã
ïŒè¬å€ã«å¯Ÿããé²åæéïŒ24æéïŒãããŠã¹ã«ã
ããL1210åã³P388çœè¡ç ã§ãããŠãã«ãã·ã³
ïŒããŠããã€ã·ã³ïŒãšæ¯èŒè©Šéšãããçµæã第
ïŒè¡šã«ç€ºãã ãè¡šã ãè¡šã (c) èçŒèª¿æ»ã«åºã¥ãè©äŸ¡
次ã«å®æœäŸã«ã€ãæ¬çºæã説æããã äŸ ïŒ ã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ããŠã¹ã»ããŒã«ã»
ãªãŠã¬ãŠã¹ïŒStreptomyces peucetius var.
aureusïŒ416F.I.æ ªïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒã®å¹
é€æ¶²ã次ã®çµæãæããåŸæå¯å€©å¹å°äžã28âã§
14æ¥éçé·ãããïŒã°ã«ã³ãŒã¹ïŒïŒ ããã¬ã¯ãŒãº
ïŒbrewerâ²sïŒä¹Ÿç¥é µæ¯1.2ïŒ ãå¡©åãããªãŠã 0.1
ïŒ ããªã«ãçé žäºæ°ŽçŽ äžã«ãªãŠã 0.05ïŒ ãçé žã«
ã«ã·ãŠã 0.1ïŒ ãç¡«é žãã°ãã·ãŠã 0.005ïŒ ãç¡«é ž
第äžéã»7H2O0.0005ïŒ ãç¡«é žäºéã»7H2O0.0005
ïŒ ãç¡«é žé ã»5H2O0.0005ïŒ ãå¯å€©ïŒïŒ ãæ°Žéæ°Ž
ã§å šé100mlãšãããPH6.7ããªãŒãã¯ã¬ãŒãäž
115âã20åå ç±ããããšã«ããæ» èããã ããããŠåŸãå¹é€æ¶²ã®èåãéããç¡èèžæºæ°Ž
ïŒmläžã«æžæ¿ããããããããŠåŸãæžæ¿æ¶²ã次ã®
液äœçé·å¹å°60mlãæãã300mlã®ãšãŒã¬ã³ãã€
ã€ãŒãã©ã¹ã³äžã«æ¥çš®ããïŒãã¬ã¯ãŒã¹ä¹Ÿç¥é µæ¯
0.3ïŒ ããããã³0.5ïŒ ãç¡é žã«ã«ã·ãŠã ã»
4H2O0.05ïŒ ãæ°Žéæ°Žã§1000mlã«ããããªãŒãã¯
ã¬ãŒãäž120âã§20åå ç±ããŠæ» èãããæ» èåŸ
ã®ãã®å¹å°ã®PHã¯6.8ã7.0ã§ããã æ¥çš®ãããã©ã¹ã³ã250r.p.m.ã§ãã€çŽåŸïŒcm
ã®åãæããŠå転æ¯åæ©äžã28âã®æž©åºŠã§ïŒæ¥é
æ¯ã€ããåèšã®ããã«çé·ãããåã ã®å¹é€æ¶²
1.5mlã次ã®çç£å¹å°50mlãæãã300mlãšãŒã¬ã³
ãã€ã€ãŒãã©ã¹ã³ã«æ¥çš®ããïŒã°ã«ã³ãŒã¹ïŒïŒ ã
ãã¬ã¯ãŒã¹ä¹Ÿç¥é µæ¯ïŒïŒ ãå¡©åãããªãŠã 0.2ïŒ ã
ãªã«ãçé žäºæ°ŽçŽ äžã«ãªãŠã 0.1ïŒ ãçé žã«ã«ã·
ãŠã 0.2ïŒ ãç¡«é žãã°ãã·ãŠã 0.01ïŒ ãç¡«é žç¬¬äž
éã»7H2O0.001ïŒ ãç¡«é žäºéã»7H2O0.001ïŒ ãç¡«
é žé ã»5H2O0.001ïŒ ãæ°Žéæ°Žã§å šé100mlãšããã
PH6.7ããªãŒãã¯ã¬ãŒãäžã115âã§20åéå ç±ã
ãããšã«ããæ» èããããã®ããã«æ¥çš®ãããã©
ã¹ã³ããåèšçºèœå·¥çšãšåãæ¡ä»¶ã§28âã§ïŒæ¥é
ææž©ä¿æããã 24æééé µæã«ãåãã©ã¹ã³ã«ã¹ã«ãã¢ãã«ã¢
ããã0.5ïœ/ã®æ¿åºŠã§æ·»å ããã48æééé µæ
ã«ãæŽã«åãã©ã¹ã³ã«ãåãç©è³ªãïŒïœ/ã®æ¿
床ã§æ·»å ããã äœçšååç©ã®æ倧æ¿åºŠã¯ãéé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒ
æ¥ã®éã«ã70mcgïŒmlã®çç£ã§éããã äŸ ïŒ äŸïŒã®èšèŒãšåæ§ã«ããŠ416F.I.æ ªã®å¹é€æ¶²ã
åŸããïŒåã®åŸæå¹å°ã®èåãæ» èèžçæ°Ž10mläž
ã«éããããããŠåŸãæžæ¿æ¶²ããäŸïŒã«èšèŒã®çº
èœå¹å°500mlãæããïŒã®ãããã«ä»ãäžžåºã
ã©ã¹ã³äžã«æ¥çš®ããããã®ãã©ã¹ã³ã120r.p.m.
ã§ãã€çŽåŸ70mmã®åãæããŠå転ããå転æ¯åæ©
äžã28âã®æž©åºŠã§48æéææž©ä¿æããã å šãŠã®çš®åããäŸïŒã«èšèŒã®çç£å¹å°50ãæ
ãã120âã§30åå ç±ããããšã«ããæ» èãã80
âäžééŒéé µåšäžã«æ¥çš®ããã24æééé µæã«
ã¹ã«ãã¢ãã«ã¢ããã0.5ïœ/ã®æ¿åºŠã§æ·»å ã
ãã48æééé µæã«æŽã«ããã®ç©è³ªãïŒïœ/ã®
æ¿åºŠã§æ·»å ãããéé µã28âã§ã230r.p.m.ã§æ¹
æããŠå®æœãã空æ°æµ0.7ïŒå¹å°ïŒminã§é
æ°ããã éé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒæ¥ã®éã«ã50mcgïŒmlã®ç
ç£ã§ãäœçšååç©ã®æ倧æ¿åºŠãéæãããã äŸ ïŒ ã¹ã«ãã¢ãã«ã¢ããã®æ·»å ãæé€ããŠãäŸïŒèš
èŒã®æäœãç¹°ãè¿ãããéé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒæ¥ã®
éã«5mcgïŒmlã®çç£ã§äœçšç©è³ªã®æ倧æ¿åºŠãé
ãããã äŸ ïŒ éé µããäŸïŒã«ããåŸãç·ããŒã«ïŒ30ïŒãå¡©
é žã§çŽïŒã®PHå€ã«èª¿ç¯ããïŒïŒ çªè»åãæãšã
ãŠçšããŠéãããšæ»åã³æ¶²ãåŸããããã
ããããå¥ã ã«æœåºããã 湿ã€ãæ»ãçŽ15ã®ã¡ã¿ããŒã«ã§æœåºããã
æœåºæ¶²ãæžå§äžã«æ¿çž®ãã次ãã§éããèæ±ãš
äžç·ã«ãããæ··åç©ãçŽ8.5ã9.0ã®PHå€ã«èª¿ç¯
ãã次ãã§åéã®ã¯ãããã«ã ã§ïŒåæœåºããã
éããææ©æœåºæ¶²ãæ°Žã§æŽæµããç¡æ°Žç¡«é žãããª
ãŠã äžã§ä¹Ÿç¥ããã次ãã§æžå§äžã«ãçŽ200mlã®
éã«ãªããŸã§æ¿çž®ããããïœâãããµã³ïŒã®æ·»
å ã«ãããç²è£œã°ãªã³ã·ããã©ã¯ã·ãšã³ã¯é»è€è²
ç²æ«ïŒ1.5ïœïŒãšããŠæ²æ®¿ããã äŸ ïŒ éé¢å¡©åºãšããŠã®ç²è£œã°ãªã³ã·ãã®ã¯ãããã«
ã 溶液ïŒ30mläžã®1.5ïœïŒã1/15ïŒçé žå¡©ç·©è¡æ¶²
ã§PHïŒã§ç·©è¡ããã¯ãããã«ã äžã§èª¿è£œããã·ãª
ã«ã²ã«ã®ã«ã©ã äžã«è£ å ¥ããããã®ã«ã©ã ãã¯ã
ããã«ã ã§æŽæµããåŸé ã¯ãããã«ã âã¡ã¿ããŒ
ã«âæ°Žâæ··åç©ã§æº¶é¢ãããã 92.2ïŒ3.5ïŒ0.2âæ··åç©ãçšãããšããããã
ã®é»è²ã¢ã°ãªã³ã³ã溶é¢ãããåŒç¶ãæçç©è³ªïŒº
ã溶é¢ãããã92.2ïŒ7.5ïŒ0.3âæ··åç©ãçšãã
溶é¢ã«ãããæçç©è³ªïŒ¹ãçãã次ãã§æçç©è³ª
ãçãããã®æº¶é¢ã¯300ïŒ55ïŒïŒâæ··åç©ãçš
ããŠéæããããçŽç²æåãå«æãããã©ã¯ã·ãš
ã³ãå°éã«ãªããŸã§æ¿çž®ããæ°Žã§çšéããPH8.5
ã9.0ã«èª¿ç¯ãã次ãã§ã¯ãããã«ã ã§æœåºããã
æ°Žã§æŽæµã®åŸã«ããã®ææ©æœåºç©ãç¡«é žãããªãŠ
ã äžã§ä¹Ÿç¥ããã次ãã§å°éã«ãªããŸã§æ¿çž®ã
ãã åœéã®ã¡ã¿ããŒã«æ§å¡©åæ°ŽçŽ ãæ·»å ãããšãå®
éã«æçç©è³ªïŒºïŒ0.04ïœïŒãæçç©è³ªïŒ¹ïŒ0.2ïœïŒå
ã³æçç©è³ªïŒ·ïŒ0.03ïœïŒã®çŽç²å¡©é žå¡©ã埮现çµæ¶
ç²æ«ãšããŠçããã æçç©è³ªïŒ·ãåã³ïŒºãã¡ã¿ããŒã«ïŒïœâãã¿
ããŒã«ããåçµæ¶ããããšãçžå¿ããçŽç²å¡©é žå¡©
ãé»æ©è²çµæ¶ãšããŠçããæçç©è³ªïŒ·ã«å¯ŸããŠè
ç¹208ã210âïŒå解ïŒãæçç©è³ªïŒ¹ã«å¯ŸããŠèç¹
195ã196âïŒå解ïŒåã³æçç©è³ªïŒºã«å¯ŸããŠèç¹
200ã201âïŒå解ïŒã瀺ããã äŸ ïŒ æçç©è³ªïŒ¹ã®è©Šæ20mgã2Nå¡©é žæ°ŽïŒmläžã«æº¶
ããã溶液ã90âã§25åéå ç±ãããçµæ¶æ§é»æ©
è²æ²æ®¿ãå·åŽåŸåãã次ãã§æ°Žã§æŽæµããäºé ž
åçäžã§ç空äžã«äžå€ä¹Ÿç¥ããããããããŠæç
ç©è³ªïŒ¹ã®ã¢ã°ãªã³ã³ã®10mgãçŽç²åœ¢ã§åŸãããã
èç¹168âãm/c368ïŒM+ïŒïŒãã®ã¢ã°ãªã³ã³ã®æ²
殿ã®åŸã«ãæ®ãã©ç¡è²ã®é žæ°Žæº¶æ¶²ã¯ãããšãŒãªã³
ã°æº¶æ¶²ãéå ãããã³ãããªã³ãšæ£ã®åå¿ããã
ååç©ãå«æãããçŽâåã³èå±€âã¯ãããã°
ã©ãã€ã«ããã°ããã®ååç©ã¯ãããŠããµãã³
ïŒïŒâã¢ããâïŒïŒïŒïŒïŒâããªããªãã·ââ
ãªããœãããœãŒã¹ïŒãå³ã¡ããŠããã€ã·ã³åã³ã¢
ããªã¢ãã€ã·ã³ã®ã¢ããç³æåã®æšæºè©Šæãšåºå¥
ãã€ããªãã
質ãéé µæ³ã«ãããã®è£œé ãç²è£œæº¶æ¶²ããã®ãã
ãæçç©è³ªã®åååã³æ¿çž®æ³ããã®ç²Ÿè£œæ³åã³ã
ã®å¡©ã®è£œæ³ã«é¢ããã æ¬çºæã«ã¯ãçšé圢ã§ãæ¿çž®ç©ãšããŠããã€çŽ
ç²çµæ¶åœ¢ã®æ°èŠæçç©è³ªãå å«ãããããããæ°
èŠã®ã¢ã³ãã©ãµã€ã¯ãªã³é¡ã¯æè «çå€ãšããŠãæ
èå€ãšããŠãæå¹ã§ããããã®æ°èŠæçç©è³ªã®äœ
çšå¹æã¯ããããã®ååŠçåã³ç©ççç¹æ§ãšå ±
ã«ãåŸæ¥å ¬ç¥ã®æçç©è³ªãšã¯ç°ãªãã æ°èŠæçç©è³ªïŒããã§ã¯æçç©è³ªïŒ·ãåã³ïŒº
ãšç§°ããïŒã¯ãã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€
ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠã¹ïŒStreptomyces
peucetius var.caeciusïŒã®å€ç°èæ ªå³ã¡ã¹ãã¬
ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ãªãŠã¬
ãŠã¹ïŒStreptomyces peucetius var.aureusïŒã®
éé µã®éã«åœ¢æãããã æ¬çºæã®æ¹æ³ã§äœ¿çšããã埮çç©ã¯ãã¹ãã¬ã
ããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠ
ã¹ãâã¡ãã«âNâ²âãããââããããœã°
ã¢ããžã³ã§ã®å€ç°èªçºåŠçã«ãã€ãŠåŸããã
ïŒARCA MONEçã«ããBiotechnol.Bioengeen
XIå·»ã1969幎ã1101ã1110é åç §ïŒãããããŠåŸ
ãæ°èŠèæ ªã¯ãã¢ã«ãã¿ãªã¢ã»ã³ã¬ã¯ã·ãšã³ã»ãª
ãã»ãã€ã¯ããªã«ã¬ãã¹ã ã¹ïŒFarmitalia
Collection of MicroorganismsïŒã®ã³ãŒãçªå·
416F.I.ãæãããã€ãããšã»ã¶ã³ã ã«ã³ã°ã»ã
ã¯ããªã«ã¬ãã¹ã¡ã³ïŒDeutsche Sammlung
MikroorganismenïŒã«å¯èšãããŠãçªå·
DSM1367ãæããã¢ã¡ãªã«ã³ã»ã¿ã€ãã»ã«ã«ã
ãŠã¢ã»ã³ã¬ã¯ã·ãšã³ïŒAmerican Type Culture
CollectionïŒã§ATCC31428ãæãã埮工ç èå¯
第4622å·ãæããã å€ãã®æçç©è³ªåœ¢æå¹é€ã®å Žåã«ããããã
ã«ãèæ ª416F.I.ã®éé µã®éã«ãæåã®æ··åç©å
ã¯è€åç©ã圢æããããïŒçš®ã®çç©ã«äœçšããã¢
ã³ãã©ãµã€ã¯ãªã³æåïŒããã§ã¯æçç©è³ªïŒ·ã
åã³ïŒºãšç§°ããïŒã¯ãåèšåŸ®çç©ã«ããçæãã
ãè€åäœããåé¢ãããŠããã æçç©è³ªïŒ·ããåã³ïŒºã¯æ¬¡ã®æ§é åŒãæãã
ããšã確èªãããïŒ ïŒæçç©è³ªïŒ· ïŒâCoâCH2âOH ïŒæçç©è³ªïŒ¹ ïŒâCoâCH3 ïŒæçç©è³ªïŒº ïŒâCH2âCH3 ããã§ãããããæçç©è³ªïŒ·()ã¯11âããªã
ã·â14âããããã·âã«ã«ãããã€ã·ã³ã«ãæç
ç©è³ªïŒ¹()ã¯11âããªãã·âã«ã«ãããã€ã·ã³
ã«ãæçç©è³ªïŒº()ã¯11âããªãã·â13âããªã
ãœâã«ã«ãããã€ã·ã³ã«ãçžåœãããåèšã®ãã
ã«ããã¹ãŠã®åèšæåã¯ãããŠããµãã³ïŒïŒâã¢
ããâïŒïŒïŒïŒïŒâããªããªãã·ââãªããœâ
ãããœãŒã¹ïŒå³ã¡ããŠããã€ã·ã³åã³ã¢ããªã¢ã
ã€ã·ã³ã®ã¢ããç³æåãå«æããã¢ã³ãã©ãµã€ã¯
ãªã³ã°ãªã³ã·ãã§ããïŒF.ArcamoneãG.
CassinelliãP.OrezziãG.Franceschiåã³R.
Mondelliã«ãããJ.Am.Chem.Soc.86å·»5335é ã
1964幎ãåã³ç±³åœç¹èš±ç¬¬3590028å·æ现æžåç §ïŒã
ãã®æåã®æ§é ã¯èµ€å€ç·ã玫å€ç·ãå¯èŠç·ã質é
ã¹ãã¯ãã«åã³æ žç£æ°å ±é³Žã¹ãã¯ãã«åæã«ãã
枬å®ãããåŸã«èšèŒã®ååŠçåã³ç©ççããŒã¿ã§
åå®ãããã æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³è€åç©åã³ãã®åã ã®
æåã¯ãé žãšãå¡©åºãšãå¡©ã圢æãããã®è€åç©
åã³æåã®è¬ç©åŠçã«èªå®¹æ§ã®å¡©ã¯ãæ¬çºæã®ç¯
å²ã«å å«ãããããã®ãããªè¬ç©åŠçã«èªå®¹æ§ã®
å¡©ã®äŸã«ã¯é žäŸãã°å¡©é žãç¡«é žãç¡é žåã³çé žãš
ã®å¡©åã³éå±éœã€ãªã³äŸãã°ã¢ã«ã«ãªéå±åã¯ã¢
ã«ã«ãªåé¡éå±éœã€ãªã³äŸãã°ãããªãŠã ãã«ãª
ãŠã ãã«ã«ã·ãŠã åã³ãã°ãã·ãŠã åã³ä»ã®éœã€
ãªã³äŸãã°ïŒäŸ¡ã®ééœã€ãªã³ãšã®å¡©ãå å«ãã
ããæ¬çºæã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ª
ã®è£œé ãåŸã«èšèŒããã 416F.I.æ ªã®é¡åŸ®é¡ç¹æ§ åºè³ªãã»ã«ã¯ãçš®ã ã®é·ãã§çŽåŸ0.5ã0.9ÎŒã®
å šäœçã«åæããè糞ã§åœ¢æãããŠãããåè ã
ãç«ã€ãŠããæ°è糞ã¯ãçŽåŸ1.1ã1.6ÎŒã®å šäœç
ã«é·ãçŽç·ç¶ãžå±æããè糞ã§åœ¢æãããŠããã
ãããããè ºç¶æã§äž¡è¶³çµåå¥æ§ã®åæããŠãè
åã¯å€åæ§ã®é·ãã®é¢ããŠæ«ç«¯ã§ã«ã®åã¯ã«ãŒã
ã«ãªã€ãŠããè糞ãæãããèåã¯ç圢ã§æ¬¡ã®å¯ž
æ³ãæããïŒçŽåŸïŒã2ÎŒããŸãé£éé 眮ãããŠ
ããã次ãã§è§£æŸãããŠãããé»åé¡åŸ®é¡äžã§ã
ãã®èåã¯äžèŠåãªèŒªéã®ã»ãŒç圢ã§ç£ç¶è¡šé¢ã
æããã 416F.I.æ ªã®èçŒçç¹æ§ 416F.I.æ ªã®å¹é€ç¹æ§ã第ïŒè¡šã«ç€ºããçé·ã¯
ææ©å¹å°äžã§ãåæå¹å°äžã§ãäžè¬ã«è¯å¥œã§ã
ãã第ïŒè¡šã«èšèŒã®å¹å°äžã®çé·èŠ³å¯ã¯ã28âã§
ææž©ä¿æ第ïŒæ¥ç®ãããçé·åæ¢ãŸã§è¡ãªã€ãã
416F.I.æ ªã®çååŠçåã³ççåŠçç¹æ§ã第ïŒè¡š
ã«ç€ºãã 40âããé«ã枩床ã§ã¯çé·ã¯èªããããªãã第
ïŒè¡šã«èšèŒã®å¹å°äžã§ã¯èæ žåœ¢æã¯èªããããªã
ã€ãã ãè¡šã ã¯ãã¯ã¹ãã³ïŒWaksmanïŒS.A.ïŒãŒã»ã¢ã¯ã
ããã€ã»ãã¹ãThe ActinomycetesïŒå·»
ïŒ1961幎ïŒWilliam Wilkins Co.Baltimoreã ããªãã ïŒPridhamïŒT.G.ãã¢ã³ããŒãœã³
ïŒAndersonïŒP.ããªã¬ã€ïŒFoleyïŒïŒ£ããªã³ãã³
ããšã«ãµãŒïŒLindenfelserïŒL.A.ããã»ã«ãã€ã³
ïŒHesseltineïŒC.W.åã³ãããã€ã¯ã
ïŒBenedictïŒR.G.ïŒã¢ã³ããŒã¬ã³ïŒAnn.ïŒ1956ïŒ
1957幎947ã953é ã®ã¢ã»ã»ã¬ã¯ã·ãšã³ã»ãªãã»ã¡
ãã€ã¢ã»ããªã¢ã»ã¡ã€ã³ãã€ãã³ã¹ã»ã¢ã³ãã»ã
ããœãããã¯ã»ã¹ã¿ãã€ã¹ã»ãªãã»ã¹ãã¬ããã
ã€ã»ã¹ã»ã¢ã³ãããªãã€ã¯ã¹ïŒïŒ¡ selection of
media for maintenance and toxonomic
studjes of Streptomyces AntibioticsïŒç¬¬ïŒè¡š 416F.I.ïŒæ ªã®ççåŠçåã³çååŠçæ§
質 ã°ã«ã³ãŒã¹å©çšæ§ ïŒ ãµãã«ããŒã¹ã â âãã·ããŒã¹ ã ïŒ ãã³ãããŒã« ã â ïœâã€ãã·ããŒã« ã ïŒ ïŒ¬âã¢ã©ãããŒã¹ ã ïŒ ïŒ€âãã©ã¯ããŒã¹ ã ïŒ ã¢ããããŒã« ã â ã©ã¯ããŒã¹ ã â ïœ(+)ãã³ããŒã¹ ã ïŒ ãã«ããŒã¹ ã ïŒ ã©ãã€ããŒã¹ ã â âã©ã ããŒã¹ ã â αâαâãã¬ãããŒã¹ ã ïŒ ãšã¹ã¯ãªã³ ã ïŒ ã°ãªã»ããŒã« ã ïŒ ã¯ãšã³é žãããªãŠã ã ïŒ ã³ãã¯é žã¢ã³ã¢ã㢠ã ïŒ é ¢é žãããªãŠã ã â é ç³é žã¢ã³ã¢ã㢠ã â ã°ãªã³ãŒã²ã³ ã ïŒ ãã©ãã€ã³ ã â ãã€ãã¹é¢ã®æ€æ»ïŒ ãŒã©ãã³ã®æ¶²å ïŒ ããã·ã³å解 ïŒ ã¡ã©ãã³åœ¢æ â ãã³ãã³ã®å æ°Žå解 ïŒ H2S圢æ ïŒ ç¡é žå¡©éå ïŒ ãã«ã¯ïŒãããã³ååã³ååºïŒ ïŒ çç£æçç©è³ªïŒæ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ ïŒïŒéœæ§åå¿ âïŒé°æ§åå¿ ïŒ äœ¿çšããçåæ°ŽçŽ å©çšæ§è©Šéšçšå¹å°ã¯ãR.
D.ãŽã«ãã³ïŒGordonïŒåã³M.L.ã¹ãã¹
ïŒSmithïŒã«ããJ.ãã¯ããªãªããžã€
ïŒBacteriologyïŒ69å·»ïŒ1955幎ïŒ147ã150é ã«
èšèŒãããŠããã ïŒ ä»ã®ççåŠçåå¿çšå¹å°ã¯ãS.A.ã¯ã¯ã¹ãã³
ïŒWaksmanïŒã«ãããããŒã»ã¢ã¯ãããã€ã»ã
ã¹ãå·»ïŒ1961幎ïŒïŒThe Williams Wilkins
Companyã»BaltimoreïŒã«å ±åãããŠããã
ã®ã§ããã 416F.I.æ ªã®åå®åã³åé¡ 416F.I.æ ªã«ãã瀺ãããå šäœçç¹æ§ã¯ãæã
ãã«ãã¹ãã¬ãããã€ã»ã¹å±ã®èã«é¢ããŠã¯ã¯ã¹
ãã³åã³ãã³ãªãïŒHenriciïŒã«ãã瀺ãããŠã
ãç¹æ§ã«çžåœãããæŽã«ã416F.I.æ ªã§ç€ºããã
圢æ åŠçãå¹é€äžåã³ççåŠçç¹æ§ã¯ãã¹ãã¬ã
ããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠ
ã¹ïŒStreptomyces peucetius var.caesiusïŒã«é¢
ããç¹æ§ïŒç±³åœç¹èš±ç¬¬3590028å·æ现æžïŒ
Arcamoneçã«ããBiotechnol.BioengeenXI
ïŒ1969幎ïŒ1101ã1110é åç §ïŒã«çžåœããããã
ãããšã¯ããã®é»è²å¯æº¶æ§è²çŽ ã®åœ¢æèœã«åºã¥
ããïœâã€ãã·ããŒã«ãâã¢ã©ãããŒã¹åã³ãš
ã¹ã¯ãªã³ãå©çšãããããµãã«ããŒã¹ããã³ãã
ãŒã«åã³ã©ãã€ããŒã¹ãå©çšããªãããšã«åºã¥
ãããã€æçµçã«ãæ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æ
çç©è³ªãçç£ããããšã«åºã¥ããç°ãªãã åŸã€ãŠã416F.I.æ ªã¯ãã¹ãã¬ãããã€ã»ã¹ã»
ããŠã»ãã€ãŠã¹ã»ããŒã«ã»ã«ãšã·ãŠã¹ã®å€çš®ã§ã
ã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»ããŒã«ã»
ãªãŠã¬ãŠã¹ïŒStreptomyces peucetius var.
aureusïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒãšåœåããã éé µæ³ ãã®æ¹æ³ã¯ãæ £çšã®å ¬ç¥æ¹æ³ã§å®æœãããçªç¶
å€ç°ãã埮çç©ããäºãæ» èãã液äœå¹å°äžã§å¥œ
æ°æ§æ¡ä»¶äžã«25âã37âïŒæå©ã«28âïŒã®æž©åºŠã§
ïŒãïŒæ¥ïŒæå©ã«ïŒæ¥ïŒéãåœå6.5ã7.0ã§ãé
é µã®çµæã«ã¯6.5ã8.0ã®PHå€ã§éé µãããããšã
ããªãã å¹å°ã¯ççŽ åã³çªçŽ æºäžŠã³ã«ç¡æ©å¡©ãããªãã ççŽ æºã¯ãäŸãã°ããã³ãã³ãããã¹ããªã³ã
ã°ã«ã³ãŒã¹ãã°ãªã»ãªã³ããã³ãããããã«ããŒ
ã¹ãã³ãŒã³ã»ã¹ãã€ãŒãã»ãªã«ãŒãå¯æº¶æ§èžæº
ç©ã倧è±æ²¹åã¯å€§è±ç²ã§ãã€ãŠãããçªçŽ æºã¯ã
çªçŽ ãå«æããåèšã®ççŽ æºä»¥å€ã«ãäŸãã°ä¹Ÿç¥
é µæ¯ãããŒããããã³åã¯ã«ãŒã€ã³ã§ãã€ãŠã
ããè¯å¥œãªçµæã¯ãã¢ã³ã¢ããŠã å¡©äŸãã°ç¡é žã¢
ã³ã¢ããŠã ãç¡«é žã¢ã³ã¢ããŠã åã³çé žäºã¢ã³ã¢
ããŠã ãçšããããšã«ãã€ãŠãåŸãããããã®ç
ç£ã«ãšã€ãŠæçšãªç¡æ©å¡©ã¯äœ¿çšå¹å°ã«äŸã€ãŠå€ã€
ãŠãããè€åç©è³ªäŸãã°çš®ã ãªç©ç²åã³å¹é€æ®æž£
ãå«æããå¹å°äžã«ãçé žã«ã«ã·ãŠã åã³çé žã
ããªãŠã åã¯çé žã«ãªãŠã ãæ·»å ããããšã¯æçš
ã§ããããšãç«èšŒããããã°ã«ã³ãŒã¹åã¯ã¢ã³ã¢
ããŠã å¡©ãå«æããå¹å°äžã«ã¯ãããé«æ¿åºŠã®ã«
ãªãŠã ããããªãŠã åã¯ã«ã«ã·ãŠã ã®ç¡æ©å¡©ãå¿
èŠã§ãããæŽã«æ¥µå°éã®éå±äŸãã°éãäºéã
é ããã°ãã·ãŠã åã³ãã³ã¬ã³ã®å¡©ãå¿ èŠã§ã
ããã¹ã«ãã¢ãã«ã¢ãããã¹ã«ãã¢ãã¢ãŸãŒã«ã
ã¹ã«ãã¢ããªããžã³ããã³ããªãã«ãã¿ãŒã«åã³
ãšããªãã³ãªã©ã®ååç©ãå«æããç¡«é»ãæ·»å ã
ãããšãæçšã§ããã éé µã¯ãšãŒã¬ã³ãã€ã€ãŒãã©ã¹ã³äžåã¯çš®ã ã®
容éã®å®éšçšåã¯å·¥æ¥çšéé µåšäžã§å®æœã§ããã åææ³ éé µèæ±åã³ç²è£œèª¿åç©ã®è©Šæãã1/15ïŒçé ž
å¡©ç·©è¡æ¶²ã§ãPH5.4ã§ç·©è¡ãããã¯ãããã³
ïŒWhatmanïŒNo.ïŒããŒããŒãçšããå±éå€ãšããŠ
ïœâãããããŒã«å¯Ÿé ¢é žãšãã«å¯Ÿæ°ŽïŒïŒïŒïŒïŒ
ïŒïŒã®æ··åç©ãçšããŠããŒããŒã¯ãããã°ã©ãã€
ã«ããããã®ããŒããŒçããã·ã«ã¹ã»ã¹ãããªã¹
ïŒBacillus subtilisïŒã«å¯ŸããŠããªãªãŒãã°ã©ã
ã€ã«ããããšãïŒçš®ã®äž»èŠæåãçŸããããšãèª
ããããããããæçç©è³ªïŒ·ïŒRf0.45ïŒãïŒRf
0.64ïŒåã³ïŒºïŒRf0.70ïŒãšåœåãããïŒçš®ã®å°é
æåãçŸããããããã¯ã°ãªã³ã·ãåã³ïŒ£ïŒã
ãããRf0.30åã³0.55ïŒãšåå®ãããç¹é¡æ54â
12850å·æ现æžã«èšèŒãããŠããã éé µèæ±äžã«ååšããå šäœçãªé»è²æåã®éç
è©äŸ¡ã¯ã次ã®æ¹æ³ã§è¡ãªãããšãã§ãããèæ±
ïŒPH8.6ã«èª¿æŽããïŒïŒè©Šæã«ãïŒåéã®ã¯ããã
ã«ã ïŒã¡ã¿ããŒã«ïŒïŒïŒïŒïŒãå ããçããæ··å
ç©ã宀枩ã§ïŒåéé³æ³¢åŠçããã次ãã§ãææ©çž
ã®è©ŠæïŒé žæ§ã¡ã¿ããŒã«ã§çšéïŒã§ãé»è²ã®ã¢ã³
ãã©ãµã€ã¯ãªã³åã³ãã®ã¢ã°ãªã³ã³ã®å šå«éã
430nmã§åå å 床枬å®ããããšãã§ãããæžå§äž
ã«æ¿çž®ãããææ©çžã®è©Šæã§ãåäžã®æçç©è³ªã®
éç枬å®ã¯ãåèšç³»ãçšãã調æŽçšèå±€ã¯ããã
ã°ã©ãã€ã§åŸãããšãã§ãããçš®ã ç°ãªãé»è²åž¯
åãæŠãåããã¡ã¿ããŒã«ã§å±éããããåæå
ã430nmã§åå å 床枬å®ãããæçç©è³ªïŒ¹ã¯ãé
äŸéé µèæ±äžã®äž»èŠæåã§ããã åé¢æ³ éé µãçµäºããåŸãæå¹æåã¯è糞äžåã³éé µ
液äžã«å«æãããŠããããã®ã¢ã³ãã©ãµã€ã¯ãªã³
ç³»æçç©è³ªã¯ãPH8.5ã9.0ã§éé¢ã®å¡©åºãšããŠå¹
é€æ¶²ããå šäœãšããŠãæ°Žãšæ··ãããªãææ©æº¶å€äŸ
ãã°ããã¿ããŒã«ãã¡ãã«ã€ãœããã«ã±ãã³ãã¯
ãããã«ã ãäºå¡©åã¡ãã¬ã³åã³é ¢é žãšãã«ã§æœ
åºããããšãã§ãããè糞åã³éé µæ¶²ãPHïŒã§çª
è»åãçšããéã«ããåé¢ãã次ãã§å¥ã ã«æœ
åºããã®ãæå©ã§ããã æ»ã氎溶æ§æº¶å€äŸãã°ã¢ã»ãã³åã³äœçŽã¢ã«
ã³ãŒã«æå©ã«ã¡ã¿ããŒã«ã§æœåºããã è糞æœåºç©ãéããæžå§äžã«æ¿çž®ããã æ¿çž®ç©ãéããèæ±ãšå ±ã«éããPH8.5ãïŒ
ã«èª¿ç¯ãã次ãã§ãæ°Žãšæ··ããããªãææ©æº¶å€ã
æå©ã«ã¯ãããã«ã åã¯ïœâãã¿ããŒã«ã§æœåºã
ããæœåºæ¶²ãæžå§äžã«æ¿çž®ãããã®ã¢ã³ãã©ãµã€
ã¯ãªã³ç³»æçç©è³ªã¯ïŒåéã®ïœâãããµã³ã®æ·»å
ã«ããæ²æ®¿ãããããšãã§ãããç²è£œæ··åç©ã®æ
åããã«ã©ã ã¯ãããã°ã©ãã€æ³ã§åå¥ã粟補ã
ãããšãã§ããã ç²Ÿè£œæ³ æçç©è³ªæŽ»æ§ãæŽã«ç²Ÿè£œãããã€ãããïŒæå
å³ã¡æçç©è³ªïŒ·ãåã³ïŒºã«åããããšã¯ãã·ãª
ã«ã²ã«ã«ã©ã ã¯ãããã°ã©ãã€ã«ããè¡ãªãããš
ãã§ãããç²è£œã®æ©è€è²ç²æ«ãã¯ãããã«ã äžã«
溶ããã溶液ããã¯ãããã«ã ïŒã¡ã¿ããŒã«ïŒæ°Ž
ã®åŸé ãçšããŠãç·©è¡ã·ãªã«ã²ã«äžã®ã¯ãããã°
ã©ãã€ã«ãããããŸãæçç©è³ªïŒºãã94.8ïŒïŒïŒ
0.2âæ··åç©ã§æº¶é¢ãããåŒç¶ã92.2ïŒ7.5ïŒ0.3â
æ··åç©ã§æçç©è³ªïŒ¹ãã次ãã§300ïŒ55ïŒïŒâæ··
åç©ã§æçç©è³ªïŒ·ã溶é¢ãããããããã®æå
ã¯ãéäŸããŒããŒã¯ãããã°ã©ãã€åã³èå±€ã¯ã
ããã°ã©ãã€ã§ç€ºãããããã«åé¢ãããæçç©
質ãåã³ïŒºã¯ãåœéã®ã¡ã¿ããŒã«æ§å¡©é žã®æ·»
å ã®éã«ãããã®å¡©é žå¡©ãšããŠãçµæ¶ã§åŸãã
ãã ååŠçåã³ç©ççç¹æ§ æçç©è³ªïŒ·ãåã³ïŒºã¯ãå ¬ç¥ã®ã¢ã³ãã©ãµã€
ã¯ãªã³ç³»æçç©è³ªã«å ±éã®ããã€ãã®ç¹æ§ã瀺ã
ãããããã¯ããã®ååŠçåã³ç©ççç¹æ§ã«åºã¥
ãåºå¥ã§ããã ããããã¹ãŠã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ã¯åæ§
ãªæº¶è§£æ§ãæããå³ã¡éé¢å¡©åºãšããŠãããã¯ã
ã¯ãããã«ã ãäºå¡©åã¡ãã¬ã³ãã¢ã»ãã³ãã¡ã¿
ããŒã«ããšã¿ããŒã«ãå«æ°Žã¢ã«ã³ãŒã«ãé žæ§æ°Žã
ãžãªããµã³åã³ããªãžã³äžã«æº¶ããããžãšãã«ãš
ãŒãã«ãïœâãããµã³ãã·ã¯ããããµã³åã³ç³æ²¹
ãšãŒãã«äžã«ã¯å ãã«å¯æº¶ã§ãããåã¯äžæº¶ã§ã
ããå¡©é žå¡©ãšããŠããããã¯æ°Žãã¡ã¿ããŒã«ããš
ã¿ããŒã«åã³å«æ°Žã¢ã«ã³ãŒã«äžã«æº¶ããã¢ã»ã
ã³ããã³ãŒã³ãã¯ãããã«ã ããžãšãã«ãšãŒãã«
åã³ç³æ²¹ãšãŒãã«äžã«ã¯äžæº¶ã§ããããããæ°èŠ
ååç©ã¯ãäžæ§åã³é žæ§æº¶æ¶²äžã§æ©é»è²ã®æ瀺è¬
ãšããŠäœ¿çšã§ãããã®æº¶æ¶²äžã§çŽ«å€ç·äžã§ã¯åž¯ç·
é»è²è¢å ãã瀺ãããã®ã¢ã«ã«ãªæº¶æ¶²ã¯èµ€è€è²ã§
ãããã¢ã«ã³ãŒã«æ§é ¢é žãã°ãã·ãŠã ã§åŠçãã
éã«ã¯æ©èµ€è²æº¶æ¶²ãçããããããã®ãã¹ãŠã®ç¹
æ§åã³çŽ«å€ç·åã³å¯èŠå ç·ã«ãããåžåã¹ãã¯ã
ã«ã¯ããããååç©ãã¢ã³ãã©ãµã€ã¯ãªã³ç³»æç
ç©è³ªã®çŸ€ã«å±ããããšã瀺ããŠããã ïŒåã®äž»æåå³ã¡å¡©é žå¡©ãšããŠåé¢ãããæç
ç©è³ªïŒ·ãåã³ïŒºã®ååŠçåã³ç©ççç¹æ§ã第ïŒ
è¡šã«ç€ºãã æçç©è³ªïŒ·ãåã³ïŒºã¯ãããã©ãµã€ã¯ãªã³ç³»
ã¢ã°ãªã³ã³ïŒã¢ã³ãã©ãµã€ã¯ãªãã³ïŒãšã¢ããç³
ã«ãã圢æãããã°ãªã³ã·ãååç©ã§ãããäŸã
ã°æçç©è³ªïŒ¹ã0.2Nå¡©é žãçšã20åé90âã§é ž
æ§å æ°Žå解ãããšãæ°Žã«äžæº¶ã®ã¢ã°ãªã³ã³ãé»è²
æ©è²éç¶ã§åŸããããèç¹168âãUVã¹ãã¯ã
ã«Î»MeOH nax227ã258ã430nmïŒE1%1cnïŒ880ã560ã
263ïŒïŒ I.R.ã¹ãã¯ãã«ïŒKBrïŒïŒ3430ã2920ã 1710ã1670ã1620ã1470ã 1450ã1420ã1385ã1355ã 1285ã1260ã1210ã1185ã 1160ã1125ã1085ã1040ã 1015ã982ã930ã900ã885ã 860åã³835cm-1. æçç©è³ªïŒ¹ã®ã¢ã°ãªã³ã³ã¯C20H16O7ã«çžåœã
ãå®éšåŒãæããïŒååé368.33ãååéã¯ã質
éã¹ãã¯ãã«ã§ç¢ºèªããïŒïœïŒe368ïŒM+ïŒïŒ332
ïŒïŒâïŒ H2OïŒïŒ317ïŒïŒâïŒ H2OâCH3ïŒåã³
289ïŒïŒâïŒ H2OâCOCH3ïŒ ä»ã®ç²Ÿè£œæçç©è³ªã®é žæ§å æ°Žå解ã«ãããïŒçš®
ã®çš®ã ã®é»è²ã¢ã°ãªã³ã³ãçãã第ïŒè¡šã«èšèŒã®
ãããªã¯ãããã°ã©ãã€ç¹æ§ã瀺ããåå®ãã
ãã 粟補æçç©è³ªïŒ·ãåã³ïŒºã®ãã¹ãŠã®é žæ§å æ°Ž
å解ã®æ°Žæ§å¯æº¶æ§ãã©ã¯ã·ãšã³ã¯ãããŠããµãã³
ïŒïŒâã¢ããâïŒïŒïŒïŒïŒâããªããªãã·ââ
ãªããœãããœãŒã¹ïŒãšåãã¯ãããã°ã©ãã€ç¹æ§
ãæããéå æ§ã¢ããç³ãå³ã¡ããŠããã€ã·ã³å
ã³ã¢ããªã¢ãã€ã·ã³ã®ã¢ããç³æåãå«æããã
ããã§ããŠããã€ã·ã³åã³ã¢ããªã¢ãã€ã·ã³ïŒã
ããã¯ããããåååã§ããïŒãšã¯ãããããã
ãŠãã«ãã·ã³åã³ãããœã«ãã·ã³ãæå³ããŠçšã
ãããŠããã ãè¡šã ãè¡šã ãè¡šã çç©åŠç掻æ§ããŒã¿ (a) æèäœçš æšæºè©Šéšç®¡çšéæ³ãçšããŠããã€ãã®åŸ®çç©
ã«å¯ŸããŠæž¬å®ããæçç©è³ªïŒ·ãåã³ïŒºïŒå¡©é ž
å¡©ãšããŠïŒã®è©Šéšç®¡å æå°æå¶æ¿åºŠïŒMICïŒ
ã第ïŒè¡šã«ç€ºãã ãè¡šã ãè¡šã (b) æè «çäœçš æ°èŠæçç©è³ªïŒ·ãåã³ïŒºããã©çŽ°è
ïŒHela cellsïŒã«å¯ŸããŠãè©Šéšç®¡å ã§è©Šéšã
ïŒè¬å€ã«å¯Ÿããé²åæéïŒ24æéïŒãããŠã¹ã«ã
ããL1210åã³P388çœè¡ç ã§ãããŠãã«ãã·ã³
ïŒããŠããã€ã·ã³ïŒãšæ¯èŒè©Šéšãããçµæã第
ïŒè¡šã«ç€ºãã ãè¡šã ãè¡šã (c) èçŒèª¿æ»ã«åºã¥ãè©äŸ¡
次ã«å®æœäŸã«ã€ãæ¬çºæã説æããã äŸ ïŒ ã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ããŠã¹ã»ããŒã«ã»
ãªãŠã¬ãŠã¹ïŒStreptomyces peucetius var.
aureusïŒ416F.I.æ ªïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒã®å¹
é€æ¶²ã次ã®çµæãæããåŸæå¯å€©å¹å°äžã28âã§
14æ¥éçé·ãããïŒã°ã«ã³ãŒã¹ïŒïŒ ããã¬ã¯ãŒãº
ïŒbrewerâ²sïŒä¹Ÿç¥é µæ¯1.2ïŒ ãå¡©åãããªãŠã 0.1
ïŒ ããªã«ãçé žäºæ°ŽçŽ äžã«ãªãŠã 0.05ïŒ ãçé žã«
ã«ã·ãŠã 0.1ïŒ ãç¡«é žãã°ãã·ãŠã 0.005ïŒ ãç¡«é ž
第äžéã»7H2O0.0005ïŒ ãç¡«é žäºéã»7H2O0.0005
ïŒ ãç¡«é žé ã»5H2O0.0005ïŒ ãå¯å€©ïŒïŒ ãæ°Žéæ°Ž
ã§å šé100mlãšãããPH6.7ããªãŒãã¯ã¬ãŒãäž
115âã20åå ç±ããããšã«ããæ» èããã ããããŠåŸãå¹é€æ¶²ã®èåãéããç¡èèžæºæ°Ž
ïŒmläžã«æžæ¿ããããããããŠåŸãæžæ¿æ¶²ã次ã®
液äœçé·å¹å°60mlãæãã300mlã®ãšãŒã¬ã³ãã€
ã€ãŒãã©ã¹ã³äžã«æ¥çš®ããïŒãã¬ã¯ãŒã¹ä¹Ÿç¥é µæ¯
0.3ïŒ ããããã³0.5ïŒ ãç¡é žã«ã«ã·ãŠã ã»
4H2O0.05ïŒ ãæ°Žéæ°Žã§1000mlã«ããããªãŒãã¯
ã¬ãŒãäž120âã§20åå ç±ããŠæ» èãããæ» èåŸ
ã®ãã®å¹å°ã®PHã¯6.8ã7.0ã§ããã æ¥çš®ãããã©ã¹ã³ã250r.p.m.ã§ãã€çŽåŸïŒcm
ã®åãæããŠå転æ¯åæ©äžã28âã®æž©åºŠã§ïŒæ¥é
æ¯ã€ããåèšã®ããã«çé·ãããåã ã®å¹é€æ¶²
1.5mlã次ã®çç£å¹å°50mlãæãã300mlãšãŒã¬ã³
ãã€ã€ãŒãã©ã¹ã³ã«æ¥çš®ããïŒã°ã«ã³ãŒã¹ïŒïŒ ã
ãã¬ã¯ãŒã¹ä¹Ÿç¥é µæ¯ïŒïŒ ãå¡©åãããªãŠã 0.2ïŒ ã
ãªã«ãçé žäºæ°ŽçŽ äžã«ãªãŠã 0.1ïŒ ãçé žã«ã«ã·
ãŠã 0.2ïŒ ãç¡«é žãã°ãã·ãŠã 0.01ïŒ ãç¡«é žç¬¬äž
éã»7H2O0.001ïŒ ãç¡«é žäºéã»7H2O0.001ïŒ ãç¡«
é žé ã»5H2O0.001ïŒ ãæ°Žéæ°Žã§å šé100mlãšããã
PH6.7ããªãŒãã¯ã¬ãŒãäžã115âã§20åéå ç±ã
ãããšã«ããæ» èããããã®ããã«æ¥çš®ãããã©
ã¹ã³ããåèšçºèœå·¥çšãšåãæ¡ä»¶ã§28âã§ïŒæ¥é
ææž©ä¿æããã 24æééé µæã«ãåãã©ã¹ã³ã«ã¹ã«ãã¢ãã«ã¢
ããã0.5ïœ/ã®æ¿åºŠã§æ·»å ããã48æééé µæ
ã«ãæŽã«åãã©ã¹ã³ã«ãåãç©è³ªãïŒïœ/ã®æ¿
床ã§æ·»å ããã äœçšååç©ã®æ倧æ¿åºŠã¯ãéé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒ
æ¥ã®éã«ã70mcgïŒmlã®çç£ã§éããã äŸ ïŒ äŸïŒã®èšèŒãšåæ§ã«ããŠ416F.I.æ ªã®å¹é€æ¶²ã
åŸããïŒåã®åŸæå¹å°ã®èåãæ» èèžçæ°Ž10mläž
ã«éããããããŠåŸãæžæ¿æ¶²ããäŸïŒã«èšèŒã®çº
èœå¹å°500mlãæããïŒã®ãããã«ä»ãäžžåºã
ã©ã¹ã³äžã«æ¥çš®ããããã®ãã©ã¹ã³ã120r.p.m.
ã§ãã€çŽåŸ70mmã®åãæããŠå転ããå転æ¯åæ©
äžã28âã®æž©åºŠã§48æéææž©ä¿æããã å šãŠã®çš®åããäŸïŒã«èšèŒã®çç£å¹å°50ãæ
ãã120âã§30åå ç±ããããšã«ããæ» èãã80
âäžééŒéé µåšäžã«æ¥çš®ããã24æééé µæã«
ã¹ã«ãã¢ãã«ã¢ããã0.5ïœ/ã®æ¿åºŠã§æ·»å ã
ãã48æééé µæã«æŽã«ããã®ç©è³ªãïŒïœ/ã®
æ¿åºŠã§æ·»å ãããéé µã28âã§ã230r.p.m.ã§æ¹
æããŠå®æœãã空æ°æµ0.7ïŒå¹å°ïŒminã§é
æ°ããã éé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒæ¥ã®éã«ã50mcgïŒmlã®ç
ç£ã§ãäœçšååç©ã®æ倧æ¿åºŠãéæãããã äŸ ïŒ ã¹ã«ãã¢ãã«ã¢ããã®æ·»å ãæé€ããŠãäŸïŒèš
èŒã®æäœãç¹°ãè¿ãããéé µã®ç¬¬ïŒæ¥ãšç¬¬ïŒæ¥ã®
éã«5mcgïŒmlã®çç£ã§äœçšç©è³ªã®æ倧æ¿åºŠãé
ãããã äŸ ïŒ éé µããäŸïŒã«ããåŸãç·ããŒã«ïŒ30ïŒãå¡©
é žã§çŽïŒã®PHå€ã«èª¿ç¯ããïŒïŒ çªè»åãæãšã
ãŠçšããŠéãããšæ»åã³æ¶²ãåŸããããã
ããããå¥ã ã«æœåºããã 湿ã€ãæ»ãçŽ15ã®ã¡ã¿ããŒã«ã§æœåºããã
æœåºæ¶²ãæžå§äžã«æ¿çž®ãã次ãã§éããèæ±ãš
äžç·ã«ãããæ··åç©ãçŽ8.5ã9.0ã®PHå€ã«èª¿ç¯
ãã次ãã§åéã®ã¯ãããã«ã ã§ïŒåæœåºããã
éããææ©æœåºæ¶²ãæ°Žã§æŽæµããç¡æ°Žç¡«é žãããª
ãŠã äžã§ä¹Ÿç¥ããã次ãã§æžå§äžã«ãçŽ200mlã®
éã«ãªããŸã§æ¿çž®ããããïœâãããµã³ïŒã®æ·»
å ã«ãããç²è£œã°ãªã³ã·ããã©ã¯ã·ãšã³ã¯é»è€è²
ç²æ«ïŒ1.5ïœïŒãšããŠæ²æ®¿ããã äŸ ïŒ éé¢å¡©åºãšããŠã®ç²è£œã°ãªã³ã·ãã®ã¯ãããã«
ã 溶液ïŒ30mläžã®1.5ïœïŒã1/15ïŒçé žå¡©ç·©è¡æ¶²
ã§PHïŒã§ç·©è¡ããã¯ãããã«ã äžã§èª¿è£œããã·ãª
ã«ã²ã«ã®ã«ã©ã äžã«è£ å ¥ããããã®ã«ã©ã ãã¯ã
ããã«ã ã§æŽæµããåŸé ã¯ãããã«ã âã¡ã¿ããŒ
ã«âæ°Žâæ··åç©ã§æº¶é¢ãããã 92.2ïŒ3.5ïŒ0.2âæ··åç©ãçšãããšããããã
ã®é»è²ã¢ã°ãªã³ã³ã溶é¢ãããåŒç¶ãæçç©è³ªïŒº
ã溶é¢ãããã92.2ïŒ7.5ïŒ0.3âæ··åç©ãçšãã
溶é¢ã«ãããæçç©è³ªïŒ¹ãçãã次ãã§æçç©è³ª
ãçãããã®æº¶é¢ã¯300ïŒ55ïŒïŒâæ··åç©ãçš
ããŠéæããããçŽç²æåãå«æãããã©ã¯ã·ãš
ã³ãå°éã«ãªããŸã§æ¿çž®ããæ°Žã§çšéããPH8.5
ã9.0ã«èª¿ç¯ãã次ãã§ã¯ãããã«ã ã§æœåºããã
æ°Žã§æŽæµã®åŸã«ããã®ææ©æœåºç©ãç¡«é žãããªãŠ
ã äžã§ä¹Ÿç¥ããã次ãã§å°éã«ãªããŸã§æ¿çž®ã
ãã åœéã®ã¡ã¿ããŒã«æ§å¡©åæ°ŽçŽ ãæ·»å ãããšãå®
éã«æçç©è³ªïŒºïŒ0.04ïœïŒãæçç©è³ªïŒ¹ïŒ0.2ïœïŒå
ã³æçç©è³ªïŒ·ïŒ0.03ïœïŒã®çŽç²å¡©é žå¡©ã埮现çµæ¶
ç²æ«ãšããŠçããã æçç©è³ªïŒ·ãåã³ïŒºãã¡ã¿ããŒã«ïŒïœâãã¿
ããŒã«ããåçµæ¶ããããšãçžå¿ããçŽç²å¡©é žå¡©
ãé»æ©è²çµæ¶ãšããŠçããæçç©è³ªïŒ·ã«å¯ŸããŠè
ç¹208ã210âïŒå解ïŒãæçç©è³ªïŒ¹ã«å¯ŸããŠèç¹
195ã196âïŒå解ïŒåã³æçç©è³ªïŒºã«å¯ŸããŠèç¹
200ã201âïŒå解ïŒã瀺ããã äŸ ïŒ æçç©è³ªïŒ¹ã®è©Šæ20mgã2Nå¡©é žæ°ŽïŒmläžã«æº¶
ããã溶液ã90âã§25åéå ç±ãããçµæ¶æ§é»æ©
è²æ²æ®¿ãå·åŽåŸåãã次ãã§æ°Žã§æŽæµããäºé ž
åçäžã§ç空äžã«äžå€ä¹Ÿç¥ããããããããŠæç
ç©è³ªïŒ¹ã®ã¢ã°ãªã³ã³ã®10mgãçŽç²åœ¢ã§åŸãããã
èç¹168âãm/c368ïŒM+ïŒïŒãã®ã¢ã°ãªã³ã³ã®æ²
殿ã®åŸã«ãæ®ãã©ç¡è²ã®é žæ°Žæº¶æ¶²ã¯ãããšãŒãªã³
ã°æº¶æ¶²ãéå ãããã³ãããªã³ãšæ£ã®åå¿ããã
ååç©ãå«æãããçŽâåã³èå±€âã¯ãããã°
ã©ãã€ã«ããã°ããã®ååç©ã¯ãããŠããµãã³
ïŒïŒâã¢ããâïŒïŒïŒïŒïŒâããªããªãã·ââ
ãªããœãããœãŒã¹ïŒãå³ã¡ããŠããã€ã·ã³åã³ã¢
ããªã¢ãã€ã·ã³ã®ã¢ããç³æåã®æšæºè©Šæãšåºå¥
ãã€ããªãã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ åŒ ïŒ»ããã§åŒã¯æçç©è³ªïŒ·ã瀺ãããã®éã¯â
COâCH2âOHãè¡šããã åŒã¯æçç©è³ªïŒ¹ã瀺ãããã®éã¯âCOâ
CH3ãè¡šããã åŒã¯æçç©è³ªïŒºã瀺ãããã®éã¯âCH2â
CH3ãè¡šãã ã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªã ïŒ 11âããªãã·â14âããããã·âã«ã«ããã
ã€ã·ã³åã³ãã®ã¢ã°ãªã³ã³ã§ããç¹èš±è«æ±ã®ç¯å²
第ïŒé èšèŒã®æçç©è³ªã ïŒ 11âããªãã·âã«ã«ãããã€ã·ã³åã³ãã®ã¢
ã°ãªã³ã³ã§ãããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ
çç©è³ªã ïŒ 11âããªãã·â13âããªããœâã«ã«ãããã€
ã·ã³åã³ãã®ã¢ã°ãªã³ã³ã§ãããç¹èš±è«æ±ã®ç¯å²
第ïŒé èšèŒã®æçç©è³ªã ïŒ æçç©è³ªïŒ·ãåã³ïŒºã®ç¡å®³ãªè¬ç©åŠçã«èª
容æ§ã®é žä»å å¡©ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒ
ã®æçç©è³ªã ïŒ åŒ ïŒ»ããã§åŒã¯æçç©è³ªïŒ·ã瀺ãããã®éã¯â
COâCH2âOHãè¡šããã åŒã¯æçç©è³ªïŒ¹ã瀺ãããã®éã¯âCOâ
CH3ãè¡šããã åŒã¯æçç©è³ªïŒºã瀺ãããã®éã¯âCH2â
CH3ãè¡šãã ã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªã補é ãã
ããã«ãã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»
ããŒã«ã»ã«ãšã·ãŠã¹ã®ïŒ®âã¡ãã«âNâ²âããã
ââããããœã°ã¢ããžã³ã§ã®å€ç°èªçºåŠçã«ã
ãåŸãããæ°èŠå€ç°èæ ªã¹ãã¬ãããã€ã»ã¹ã»ã
ãŠã»ãã€ãŠã¹ã»ããŒã«ã»ãªãŠã¬ãŠã¹ïŒ416F.I.
æ ªïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒã奜æ°æ§æ¡ä»¶äžã«ãå
åå¯èœã®ççŽ æºåã³ååå¯èœã®çªçŽ æºåã³ç¡æ©å¡©
ãå«æããæ°Žæ§å¹å°äžã§å¹é€ããããšãç¹åŸŽãšã
ããã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªã®è£œæ³ã ïŒ 25âã37âã®æž©åºŠã§ïŒãïŒæ¥éãåœåPHå€
6.5ã7.0ã§ããã€å¹é€å·¥çšã®çµãã®PHå€6.5ã8.0
ã§å®æœãããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒ è€åæçç©è³ªããå¹é€ç©ãåé¢ããè糞åã¯
æ¿Ÿéããèæ±ããæœåºãããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒ
é èšèŒã®æ¹æ³ã ïŒ åŒ ïŒ»ããã§åŒã¯æçç©è³ªïŒ·ã瀺ãããã®éã¯â
COâCH2âOHãè¡šããã åŒã¯æçç©è³ªïŒ¹ã瀺ãããã®éã¯âCOâ
CH3ãè¡šããã åŒã¯æçç©è³ªïŒºã瀺ãããã®éã¯âCH2â
CH3ãè¡šãã ã®æ°èŠã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªã補é ãã
ããã«ãã¹ãã¬ãããã€ã»ã¹ã»ããŠã»ãã€ãŠã¹ã»
ããŒã«ã»ã«ãšã·ãŠã¹ã®ïŒ®âã¡ãã«âNâ²âããã
ââããããœã°ã¢ããžã³ã§ã®å€ç°èªçºåŠçã«ã
ãåŸãããæ°èŠå€ç°èæ ªã¹ãã¬ãããã€ã»ã¹ã»ã
ãŠã»ãã€ãŠã¹ã»ããŒã«ã»ãªãŠã¬ãŠã¹ïŒ416F.I.
æ ªïŒåŸ®å·¥ç èå¯ç¬¬4622å·ïŒã奜æ°æ§æ¡ä»¶äžã«ãå
åå¯èœã®ççŽ æºåã³ååå¯èœã®çªçŽ æºåã³ç¡æ©å¡©
ãå«æããæ°Žæ§å¹å°äžã§å¹é€ããåŸãããè€åæ
çç©è³ªããã·ãªã«ã²ã«ã«ã©ã ã¯ãããã°ã©ãã€ã«
ãããã®ïŒçš®ã®ã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªïŒ
ã°ãªã³ã·ããåã³ïŒºã«åé¢ããããšãç¹åŸŽãš
ãããã¢ã³ãã©ãµã€ã¯ãªã³ç³»æçç©è³ªã®è£œæ³ã ïŒïŒ 25âã37âã®æž©åºŠã§ïŒãïŒæ¥éãåœåPHå€
6.5ã7.0ã§ããã€å¹é€å·¥çšã®çµãã®PHå€6.5ã8.0
ã§å®æœãããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒïŒ è€åæçç©è³ªããå¹é€ç©ãåé¢ããè糞å
ã¯æ¿Ÿéããèæ±ããæœåºãããç¹èš±è«æ±ã®ç¯å²ç¬¬
ïŒé èšèŒã®æ¹æ³ã
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB7845434 | 1978-11-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5576896A JPS5576896A (en) | 1980-06-10 |
| JPS6332080B2 true JPS6332080B2 (ja) | 1988-06-28 |
Family
ID=10501200
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15011179A Granted JPS5576896A (en) | 1978-11-21 | 1979-11-21 | Novel anthracycline antibiotic*its manufacture and antibacterial and antitumor agent containing same |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US4370474A (ja) |
| JP (1) | JPS5576896A (ja) |
| CH (1) | CH648565A5 (ja) |
| DE (1) | DE2946793C2 (ja) |
| FR (2) | FR2442242A1 (ja) |
| IT (1) | IT1196402B (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH648327A5 (de) * | 1980-10-16 | 1985-03-15 | Hoffmann La Roche | Anthracycline. |
| DE3422735C2 (de) * | 1983-06-23 | 1996-07-11 | Erba Carlo Spa | Anthracyclinglykoside, Verfahren zu deren Herstellung und Arzneimittel, welche diese enthalten |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| YU33730B (en) * | 1967-04-18 | 1978-02-28 | Farmaceutici Italia | Process for preparing a novel antibiotic substance and salts thereof |
| US4039663A (en) * | 1975-03-19 | 1977-08-02 | Societa' Farmaceutici Italia S.P.A. | Daunomycins, process for their uses and intermediates |
| GB1506200A (en) * | 1975-04-30 | 1978-04-05 | Farmaceutici Italia | Glycosides |
| US4039736A (en) * | 1976-04-15 | 1977-08-02 | Bristol-Myers Company | Antibiotic compounds marcellomycin and musettamycin |
| NL7900869A (nl) * | 1978-02-09 | 1979-08-13 | Erba Farmitalia | Anthracyclinenantibiotica. |
| US4309503A (en) * | 1978-02-09 | 1982-01-05 | Farmitalia Carlo Erba S.P.A. | Preparation of 11-deoxy anthracycline antibiotics |
| US4263428A (en) * | 1978-03-24 | 1981-04-21 | The Regents Of The University Of California | Bis-anthracycline nucleic acid function inhibitors and improved method for administering the same |
| JPS5648893A (en) * | 1979-09-07 | 1981-05-02 | Sanraku Inc | Preparation of anthracycline glycoside |
-
1979
- 1979-11-09 IT IT27158/79A patent/IT1196402B/it active
- 1979-11-15 US US06/094,671 patent/US4370474A/en not_active Expired - Lifetime
- 1979-11-16 FR FR7928290A patent/FR2442242A1/fr active Granted
- 1979-11-20 DE DE2946793A patent/DE2946793C2/de not_active Expired
- 1979-11-20 CH CH10358/79A patent/CH648565A5/de not_active IP Right Cessation
- 1979-11-21 JP JP15011179A patent/JPS5576896A/ja active Granted
-
1980
- 1980-04-04 FR FR8007729A patent/FR2450801A1/fr active Granted
-
1981
- 1981-11-16 US US06/321,558 patent/US4421851A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| FR2450801B1 (ja) | 1983-09-23 |
| FR2442242B1 (ja) | 1983-11-10 |
| US4370474A (en) | 1983-01-25 |
| IT7927158A0 (it) | 1979-11-09 |
| DE2946793A1 (de) | 1980-07-03 |
| JPS5576896A (en) | 1980-06-10 |
| IT1196402B (it) | 1988-11-16 |
| FR2442242A1 (fr) | 1980-06-20 |
| US4421851A (en) | 1983-12-20 |
| CH648565A5 (de) | 1985-03-29 |
| FR2450801A1 (fr) | 1980-10-03 |
| DE2946793C2 (de) | 1985-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3988315A (en) | Antibiotics aclacinomycins A and B | |
| DE3887820T2 (de) | Antibiotika, Benanomicine A und B und Dexylosylbenanomicin B, ihre Herstellung und Verwendung. | |
| US4207313A (en) | Anthracycline antibiotics | |
| EP0131181B1 (de) | Anthracyclin-Derivate, ein mikrobiologisches Verfahren zu ihrer Herstellung und ihre Verwendung als Cytostatika | |
| US4247545A (en) | 11-Deoxy anthracycline antibiotics, their preparation and use | |
| US4209588A (en) | Process for producing new antitumor anthracycline antibiotics | |
| US4373094A (en) | Anthracycline derivatives | |
| US4012284A (en) | Process of preparation of antibiotic F.I. 1762 derivatives | |
| US4198480A (en) | Process for producing antibiotic baumycin complex and components | |
| US4127714A (en) | Anthracycline glycosides | |
| DE3012565A1 (de) | Antitumor-antibakterielle mittel | |
| DK146342B (da) | Fremgangsmaade til fremstilling af anthracyclinglycosidantibiotika betegnet ma 144-m1 og ma 144-m2 eller ikke toksiske syreadditionssalte eller komplekser deraf med desoxyribonucleinsyre | |
| US4309503A (en) | Preparation of 11-deoxy anthracycline antibiotics | |
| JPS6332080B2 (ja) | ||
| US4209511A (en) | Aminoglycoside antibiotics and process for production thereof | |
| US4942155A (en) | Biosynthetic anthracyclines related to daunorubicin | |
| US5109122A (en) | Antibiotics, dexylosylbenanomicin B | |
| US4411834A (en) | Preparation of II-deoxy anthracycline antibiotics | |
| US4071411A (en) | Antitumor antibiotics aclacinomycins A and B [RD-9187A] | |
| US4440751A (en) | Broad spectrum antibiotic complex produced by a novel micromonospora | |
| GB2048245A (en) | A biosynthetic anthracycline glycoside | |
| GB2036021A (en) | Antitumor antibiotics | |
| EP0167935B1 (en) | Anthracycline derivatives, a process for the production thereof, a pharmaceutical composition containing the same and their use as medicaments | |
| US4192915A (en) | Anthracycline glycosides from streptomyces | |
| DD220046A1 (de) | Verfahren zur herstellung von beta-rhodomycin ii sowie seines aglykons |