JPS63193039A - Method for analyzing medicinal components of ginseng - Google Patents
Method for analyzing medicinal components of ginsengInfo
- Publication number
- JPS63193039A JPS63193039A JP2602687A JP2602687A JPS63193039A JP S63193039 A JPS63193039 A JP S63193039A JP 2602687 A JP2602687 A JP 2602687A JP 2602687 A JP2602687 A JP 2602687A JP S63193039 A JPS63193039 A JP S63193039A
- Authority
- JP
- Japan
- Prior art keywords
- tank
- sugar
- ginseside
- specimen
- hydrolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 5
- 235000008434 ginseng Nutrition 0.000 title claims description 4
- 241000208340 Araliaceae Species 0.000 title 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title 1
- 235000003140 Panax quinquefolius Nutrition 0.000 title 1
- 240000004371 Panax ginseng Species 0.000 claims description 3
- 235000002789 Panax ginseng Nutrition 0.000 claims description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 2
- 229930064664 L-arginine Natural products 0.000 claims description 2
- 235000014852 L-arginine Nutrition 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 abstract description 11
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 abstract description 10
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 9
- 230000007062 hydrolysis Effects 0.000 abstract description 9
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 9
- 239000002253 acid Substances 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 5
- 239000000243 solution Substances 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 3
- 239000000783 alginic acid Substances 0.000 abstract description 2
- 229960001126 alginic acid Drugs 0.000 abstract description 2
- 229920000615 alginic acid Polymers 0.000 abstract description 2
- 238000001816 cooling Methods 0.000 abstract description 2
- 238000002347 injection Methods 0.000 abstract description 2
- 239000007924 injection Substances 0.000 abstract description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- 241000234314 Zingiber Species 0.000 description 2
- 235000006886 Zingiber officinale Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 235000009697 arginine Nutrition 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002795 fluorescence method Methods 0.000 description 2
- 235000008397 ginger Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
【発明の詳細な説明】
（技術分野）
本発明は、朝鮮ニンジンに含まれている薬効成分、いわ
ゆるジンセサイドを分析する技術に関する。DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to a technique for analyzing medicinal ingredients contained in Korean ginseng, so-called ginsesides.
（従来技術）
ジンセサイドの分析は、高速液体クロマトグラフを使用
して各成分に分離した稜、紫外分光光度法により検出す
ることが（Ｊ、、Ｍｅｄｉｃａｌ Ｐｌａｕｔ Ｒｅｓ
。(Prior Art) Ginsside can be analyzed by separating each component using high-performance liquid chromatography and detecting it by ultraviolet spectrophotometry (J., Medical Plaut Res.
.
３８、１９８０年）行なわれているが、これら成分によ
る吸収波長は２０５ ｎｍという短波長であるため、分
子内に吸収に関与する部分が少くて検出感度が低いばか
りでなく、第３図に示したように夾雑物の影響を受は易
いという問題があった。38, 1980), but since the absorption wavelength by these components is as short as 205 nm, the detection sensitivity is not only low because there are few parts in the molecule that are involved in absorption, but also the detection sensitivity is low, as shown in Figure 3. As mentioned above, there was a problem that it was easily affected by foreign substances.
（目的）
本発明はこのような問題に鑑みてなされたものであって
、その目的とするところは検出感度が高く、しかも夾雑
成分の影響を受けることのない朝鮮ニンジンの薬効成分
の分析方法を提案することにある。(Purpose) The present invention was made in view of these problems, and its purpose is to provide a method for analyzing the medicinal components of Korean ginseng that has high detection sensitivity and is not affected by contaminant components. It's about making suggestions.
（発明の概要）
すなわち、本発明が特徴とするところは、ジンセサイド
を酸による加水分解により糖に変換し、これを蛍光法に
より検出するようにした点にある。(Summary of the Invention) That is, the present invention is characterized in that ginseside is converted into sugar by hydrolysis with an acid, and this is detected by a fluorescence method.
（実施例）
そこで以下に本発明の詳細を実施例に基づいて説明する
。(Example) Therefore, the details of the present invention will be explained below based on an example.
第１図は本発明に使用する装置の一実施例を示すもので
あって、図中符号１はシリカ粒子の表面にオクタデシル
墨ヲ化学結合してなる分離用カラムで、一端は試料注入
口２を介して移動相タンク３に、また他端は分岐管を介
して加水分解用酸液タンク４に接続され、ざらに温度１
４０乃至１６０℃程度に保持された混合コイル５を介し
て蛍光化試薬液タンク６に接続されている。これの流出
側には冷却槽７を介して蛍光検出器８が接続されでいる
。なあ、図中符号９．１０．１１は、それぞれ送液ポン
プを示す。FIG. 1 shows an embodiment of the apparatus used in the present invention, in which reference numeral 1 is a separation column made of octadecyl ink chemically bonded to the surface of silica particles, and one end is a sample injection port 2. The other end is connected to the mobile phase tank 3 via a branch pipe, and the other end is connected to the hydrolysis acid liquid tank 4 via a branch pipe.
It is connected to a fluorescent reagent liquid tank 6 via a mixing coil 5 maintained at about 40 to 160°C. A fluorescence detector 8 is connected to the outlet side of this via a cooling tank 7. Incidentally, symbols 9, 10, and 11 in the figure indicate liquid pumps, respectively.
このように構成した装置において、移動相タンク３にア
セトニトリルと水を混合してなる移動相を、また加水分
解用酸液タンク４に過塩素酸水を、ざらに蛍光化試薬タ
ンクにホウ酸５％、Ｌ−アルギニン酸１％、水酸過ナト
リウム１２８ｍモルを水に溶解したものを収容して装置
を作動させる。In the apparatus configured in this manner, a mobile phase consisting of a mixture of acetonitrile and water is placed in the mobile phase tank 3, perchloric acid water is placed in the acid solution tank 4 for hydrolysis, and boric acid 5 is placed in the fluorescent reagent tank. %, L-alginic acid 1%, and 128 mmol persodium hydroxide dissolved in water.
このような状態で試料を注入すると、試料に含まれてい
る各ジンセッサイドは、固定相において分離されたのち
、過塩素酸により加水分解を受けで糖に変換される。こ
れらの糖は、蛍光化試薬により蛍光化されて検出器８に
流入し、濃度に比例した蛍光を発する（Ｂｕｎｓｅｋｉ
ｋａｃ＋ａｋｕ Ｖｏｌ、３２．１９８３年）、なお
、試料中に含まれている他の糖成分は、カラム１におい
てジンセサイド成分と分離されるため、ジンセサイドの
検出に妨害を与えるようなことはない。When a sample is injected in this state, the ginssides contained in the sample are separated in the stationary phase and then hydrolyzed by perchloric acid and converted into sugars. These sugars are fluoresced by a fluorescing reagent, flow into the detector 8, and emit fluorescence proportional to the concentration (Bunseki et al.
(Kac+Aku Vol. 32, 1983), since other sugar components contained in the sample are separated from the ginger component in column 1, they do not interfere with the detection of the ginger.
［実 施 例］
水６０％、アセトニトリル４０％の混合液を移動相に、
また水素イオン濃度０．８２となる濃度の過塩素酸水を
加水分解液に、ざら硼酸５％、Ｌ−アルギニン１％、水
酸化ナトリウム１２８ｍモルを混合したものを蛍光化試
薬に使用してそれぞれ０．３〜１．０ｍβ／分の割合で
供給するとともに、混合コイル５の反応温度１５０℃に
保持した状態で、ジンセサイドのＲｐｔ、Ｒｂｔをそれ
ぞれ５ｍｑ含有する試料を分析したところ、第２図に示
したように、各ジンセサイド成分を分離した状態で検出
することができた。[Example] A mixture of 60% water and 40% acetonitrile was used as the mobile phase.
In addition, a mixture of perchloric acid water with a hydrogen ion concentration of 0.82 as a hydrolysis solution, 5% boric acid, 1% L-arginine, and 128 mmol of sodium hydroxide was used as a fluorescent reagent. When a sample containing 5 mq each of ginsicide Rpt and Rbt was analyzed while supplying at a rate of 0.3 to 1.0 mβ/min and maintaining the reaction temperature of the mixing coil 5 at 150°C, the results shown in Figure 2 were obtained. As shown, each ginsside component could be detected in a separated state.
なお、移動相の純水の混合比率を３０〜８０％で、また
蛍光化試薬として硼酸を１〜５％、し−アルギニン７Ａ
０．１〜５％、水酸化ナトリウムを１２０〜３００ｍモ
ルの範囲で変更したが検出感度に大きな影響のないこと
が確認できた。In addition, the mixing ratio of pure water as a mobile phase was 30 to 80%, boric acid was 1 to 5% as a fluorescent reagent, and arginine 7A was used as a fluorescent reagent.
Although the amount of sodium hydroxide was changed in the range of 0.1 to 5% and 120 to 300 mmol, it was confirmed that the detection sensitivity was not significantly affected.
（９ｊＪ果）
以上説明したように本発明によれば、ジンセサイドを酸
による加水分解により糖に変換し、これにし−アルギニ
ンを作用させるようにしたので、ジンセサイド成分を蛍
光法により検出することができ、波長の短い紫外線によ
る夾雑物の影響を排除して検出感度のと測定精度の向上
、を図ることができる。(9jJ fruit) As explained above, according to the present invention, since ginsside is converted into sugar by hydrolysis with acid and arginine is made to act on this, the ginsside component can be detected by fluorescence method. It is possible to improve detection sensitivity and measurement accuracy by eliminating the influence of contaminants caused by short-wavelength ultraviolet rays.
第１図は本発明に使用する装置の一例を示す構成図、第
２図は同上装置による測定結果の一例を示すクロマトグ
ラムの図、及び第３図は従来方法によるシンセサイドの
測定結果を示すクロマトグラムの図である。
１・・・・分離用カラム ３・・・・移動相タンク４
・・・・加水分解用酸液タンク
６・・・・蛍光化試薬タンク
８・・・・蛍光検出器
第１図
りｌＩ？ｂｌFig. 1 is a block diagram showing an example of the apparatus used in the present invention, Fig. 2 is a chromatogram showing an example of the measurement results by the same apparatus, and Fig. 3 shows the measurement results of synthesized by the conventional method. FIG. 2 is a diagram of a chromatogram. 1... Separation column 3... Mobile phase tank 4
... Hydrolysis acid solution tank 6 ... Fluorescence reagent tank 8 ... Fluorescence detector first drawing lI? bl
Claims (1)
ド成分を加水分解する工程と、前記工程により生成した
成分にＬ−アルギニンを反応させて蛍光強度を検出する
工程とからなる朝鮮ニンジンの薬効成分分析方法。A method for analyzing medicinal components of Korean ginseng, which comprises the steps of: hydrolyzing the ginsside component separated by high-performance liquid chromatography; and reacting the component produced in the step with L-arginine to detect fluorescence intensity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2602687A JPS63193039A (en) | 1987-02-06 | 1987-02-06 | Method for analyzing medicinal components of ginseng |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2602687A JPS63193039A (en) | 1987-02-06 | 1987-02-06 | Method for analyzing medicinal components of ginseng |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63193039A true JPS63193039A (en) | 1988-08-10 |
Family
ID=12182190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2602687A Pending JPS63193039A (en) | 1987-02-06 | 1987-02-06 | Method for analyzing medicinal components of ginseng |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63193039A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2139526A1 (en) * | 1997-12-02 | 2000-02-01 | Union Tostadora S A | Analytical method for the quantitative determination of ginsenosides in a sample |
| CN1325911C (en) * | 2004-03-09 | 2007-07-11 | 中国医学科学院药用植物研究所 | Method for detecting sample containing trace notoginseng triterpene |
| CN102707000A (en) * | 2012-03-14 | 2012-10-03 | 周永刚 | Determination method of content of ginsenoside Rg1 in Senxianling particles |
| CN103575667A (en) * | 2012-07-24 | 2014-02-12 | 长白山制药股份有限公司 | Method for determining total saponins content in pharmaceutical composition injection |
| KR102332068B1 (en) * | 2020-11-20 | 2021-12-01 | 주식회사 파미니티 | Index material for classification of Korean ginseng and classification of Korean ginseng species using the same |
-
1987
- 1987-02-06 JP JP2602687A patent/JPS63193039A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2139526A1 (en) * | 1997-12-02 | 2000-02-01 | Union Tostadora S A | Analytical method for the quantitative determination of ginsenosides in a sample |
| CN1325911C (en) * | 2004-03-09 | 2007-07-11 | 中国医学科学院药用植物研究所 | Method for detecting sample containing trace notoginseng triterpene |
| CN102707000A (en) * | 2012-03-14 | 2012-10-03 | 周永刚 | Determination method of content of ginsenoside Rg1 in Senxianling particles |
| CN103575667A (en) * | 2012-07-24 | 2014-02-12 | 长白山制药股份有限公司 | Method for determining total saponins content in pharmaceutical composition injection |
| KR102332068B1 (en) * | 2020-11-20 | 2021-12-01 | 주식회사 파미니티 | Index material for classification of Korean ginseng and classification of Korean ginseng species using the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101782587A (en) | Instrument system suitable for synchronous on-line detection of adsorption spectrum and fluorescence spectrum | |
| CN113248512B (en) | Fluorescent probe for detecting trace water in organic solvent and preparation method and application thereof | |
| CN105092739A (en) | Method for measuring seven kinds of organic acid in rice wine by adopting solid-phase extraction-liquid-phase chromatogram method | |
| Yoshimura et al. | Microdetermination of phosphate in water by gel-phase colorimetry with molybdenum blue | |
| Helmchen et al. | Determination of optical purity by high performance liquid chromatography | |
| CN105891374A (en) | Detection method for measuring eight preservatives in reconstituted tobaccos simultaneously | |
| Herve et al. | Determination of thiamine and its phosphate esters in human erythrocytes by high-performance liquid chromatography with isocratic elution | |
| US6855555B2 (en) | Method for simultaneous analysis of saccharide mixture and analytical apparatus system therefor | |
| JPS63193039A (en) | Method for analyzing medicinal components of ginseng | |
| Todoriki et al. | Sensitive high-performance liquid chromatographic determination of catecholamines in rat brain using a laser fluorimetric detection system | |
| CN114705768A (en) | Sample pretreatment for detecting content of chloral hydrate in health product and detection method thereof | |
| CN101625340A (en) | High performance liquid chromatogram detecting method for detecting metaphenylene diamine in cosmetic oxidizing-type hair colorant | |
| Leeson et al. | A method for testing the purity of commercial samples of T 1824 with observations on the amounts of coloured impurities present and the errors in plasma volume estimation caused by them | |
| JPS6188148A (en) | Pretreatment of catecholamine for high-speed liquid chromatography using fluorescent spectroscopic detector | |
| CN116678982B (en) | Detection method of paliperidone palmitate impurity SM1-G | |
| Miller et al. | Spectrophotometric determination of manganese in human plasma and red cells with benzohydroxamic acid | |
| CN110907572B (en) | Device and method for measuring 8-isomeric prostaglandin F2 alpha in urine | |
| Schwedt | Liquid Column Chromatography with Chemical Derivatizations after Separation.[New analytical methods (15)] | |
| JP2000097852A (en) | Ammonia measuring device | |
| JPS63124958A (en) | Analysis of aromatic amino acid by high performance liquid chromatograph | |
| CN111323497B (en) | Optical purity analysis method of pasireotide starting material | |
| Han et al. | Cetyltrimethylammonium bromide-enhanced chemiluminescence determination of uric acid using a luminol-hexacyanoferrate (III)-hexacyanoferrate (II) system | |
| SU1550376A1 (en) | Method of refractometric detecting in microcolumn liquid chromatography | |
| KR100477307B1 (en) | Simultaneous Analysis of Vitamins B1, B2, and B6 Using Partial Post-Column Reactions | |
| JPS59100856A (en) | Analysis device connecting liquid chromatograph and mass spectrometer |