JPS63101302A - Culture of plant - Google Patents
Culture of plantInfo
- Publication number
- JPS63101302A JPS63101302A JP61245400A JP24540086A JPS63101302A JP S63101302 A JPS63101302 A JP S63101302A JP 61245400 A JP61245400 A JP 61245400A JP 24540086 A JP24540086 A JP 24540086A JP S63101302 A JPS63101302 A JP S63101302A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- alginic acid
- oligosaccharide
- alginate
- useful plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 58
- 229920000615 alginic acid Polymers 0.000 claims abstract description 58
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 54
- -1 alginic acid oligosaccharide Chemical class 0.000 claims abstract description 37
- 229940072056 alginate Drugs 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 8
- 244000045561 useful plants Species 0.000 claims description 3
- 229960001126 alginic acid Drugs 0.000 abstract description 39
- 239000000783 alginic acid Substances 0.000 abstract description 39
- 150000004781 alginic acids Chemical class 0.000 abstract description 19
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 18
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 abstract description 13
- AEMOLEFTQBMNLQ-VANFPWTGSA-N D-mannopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-VANFPWTGSA-N 0.000 abstract description 11
- 108090000790 Enzymes Proteins 0.000 abstract description 9
- 102000004190 Enzymes Human genes 0.000 abstract description 9
- 239000002253 acid Substances 0.000 abstract description 9
- 239000000203 mixture Substances 0.000 abstract description 9
- 239000002689 soil Substances 0.000 abstract description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 8
- 239000003337 fertilizer Substances 0.000 abstract description 7
- 230000012010 growth Effects 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 6
- 239000007864 aqueous solution Substances 0.000 abstract description 5
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 150000004804 polysaccharides Chemical class 0.000 abstract description 4
- 230000003301 hydrolyzing effect Effects 0.000 abstract description 3
- 238000006116 polymerization reaction Methods 0.000 abstract description 3
- 241000195493 Cryptophyta Species 0.000 abstract description 2
- 108090000856 Lyases Proteins 0.000 abstract description 2
- 102000004317 Lyases Human genes 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 244000038559 crop plants Species 0.000 abstract 4
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 abstract 2
- 229940097043 glucuronic acid Drugs 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 239000003501 hydroponics Substances 0.000 abstract 1
- 159000000000 sodium salts Chemical class 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 10
- 229940005550 sodium alginate Drugs 0.000 description 10
- 239000000661 sodium alginate Substances 0.000 description 10
- 235000010413 sodium alginate Nutrition 0.000 description 10
- AEMOLEFTQBMNLQ-BZINKQHNSA-N D-Guluronic Acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@@H](O)[C@H]1O AEMOLEFTQBMNLQ-BZINKQHNSA-N 0.000 description 8
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 108010004131 poly(beta-D-mannuronate) lyase Proteins 0.000 description 7
- 241000512259 Ascophyllum nodosum Species 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 241001474374 Blennius Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000012271 agricultural production Methods 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 229920003002 synthetic resin Polymers 0.000 description 2
- 239000000057 synthetic resin Substances 0.000 description 2
- 210000002268 wool Anatomy 0.000 description 2
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical group C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 1
- AWFYPPSBLUWMFQ-UHFFFAOYSA-N 2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]-1-(1,4,6,7-tetrahydropyrazolo[4,3-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NN=C(O1)CC(=O)N1CC2=C(CC1)NN=C2 AWFYPPSBLUWMFQ-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 244000146493 Cryptotaenia japonica Species 0.000 description 1
- 235000004035 Cryptotaenia japonica Nutrition 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001469654 Lawsonia <weevil> Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- IHPVFYLOGNNZLA-UHFFFAOYSA-N Phytoalexin Natural products COC1=CC=CC=C1C1OC(C=C2C(OCO2)=C2OC)=C2C(=O)C1 IHPVFYLOGNNZLA-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000000280 phytoalexin Substances 0.000 description 1
- 150000001857 phytoalexin derivatives Chemical class 0.000 description 1
- 230000004983 pleiotropic effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、アルギン酸オリゴ糖を植物または土壌等に施
用することにより植物の生長を促進し、農作物の効率的
生産を可能とする方法を提供するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention provides a method for promoting the growth of plants and enabling efficient production of agricultural products by applying alginic acid oligosaccharides to plants, soil, etc. It is something to do.
（従来の技術とその問題点）
農作物の生長を促進し、単位面積当りの収１！量を増し
、さらには作付回数を増やすことによって増収を計るこ
とは農業生産上重要な課題である。(Conventional technology and its problems) Promotes the growth of crops and increases yield per unit area! Increasing yields by increasing the amount and the number of plantings is an important issue in agricultural production.
植物の生長促進物質としては、従来からジベレリン、オ
ーキシン等の植物ホルモンが報告されているが、これら
の植物ホルモンの作用は多面的であり、植物に対して同
時に多くの作用をもたらすことから、ある作用が有益で
あっても、別の作用はむしろ有害な場合もあり、実用的
には用途が限定されている。Plant hormones such as gibberellins and auxins have been reported as plant growth-promoting substances, but the effects of these plant hormones are multifaceted and have many effects on plants at the same time. Even if one effect is beneficial, another effect may be harmful, and its practical use is limited.
一方、近年になって植物体の細胞壁を構成する多糖体の
分解によって得られるオリゴ糖が植物自体の生体防御反
応や分化誘導などの調節物質として重要な役割を持って
いることが報告されるようになって来た。On the other hand, in recent years, it has been reported that oligosaccharides obtained by the decomposition of polysaccharides that make up the cell walls of plants play an important role as regulators of the plant's own biological defense response and induction of differentiation. It has become.
例えば、植物細胞壁から調製されたオリゴガラクツロン
酸は、ダイズに作用させると、ある種の抗菌物質（ファ
イトアレキシン）の合成を促進し、病原菌に対する抵抗
性が増強されるし、カエデの細胞壁から調製されたオリ
ゴｔＪ！ｉ （キシロオリゴ糖）は、エントウの芽生え
に対するオーキシンの生長促進作用を逆に抑制する効果
があることが報告されている。For example, when applied to soybean, oligogalacturonic acid prepared from plant cell walls promotes the synthesis of certain antibacterial substances (phytoalexins) and enhances resistance to pathogenic bacteria; Oligo tJ! It has been reported that i (xylooligosaccharide) has the effect of conversely suppressing the growth-promoting effect of auxin on the sprouting of Japanese bean sprouts.
このようなオリゴ糖の作用は、植物ホルモンとは異なり
、その作用は多面的であるというよりは、むしろ特異的
であるとされている。The actions of such oligosaccharides are different from those of plant hormones, and their actions are said to be specific rather than pleiotropic.
本発明の目的は、種々のオリゴ糖の中から植物の生長を
促進する作用を有する糖を見出し、このオリゴ糖を農業
生産に応用し、農業生産の効率化をはかることにある。An object of the present invention is to discover sugars that promote plant growth from among various oligosaccharides, apply these oligosaccharides to agricultural production, and improve the efficiency of agricultural production.
本発明者らは、上記目的を達成させるため、植物の生長
を促進する作用を存するオリゴ糖について広く検索し、
アルギン酸を分解して得られるオリゴ糖（以下、アルギ
ン酸オリゴ糖と称する。）に植物の根および地上部の生
育を促進する作用があることを新たに見出し、本発明を
完成した。すなわち本発明は、アルギン酸オリゴ糖を用
いることを特徴とする有用植物の栽培方法である。In order to achieve the above object, the present inventors conducted a wide search for oligosaccharides that have the effect of promoting plant growth.
The present invention was completed based on the new discovery that oligosaccharides obtained by decomposing alginic acid (hereinafter referred to as alginic acid oligosaccharides) have the effect of promoting the growth of roots and aerial parts of plants. That is, the present invention is a method for cultivating useful plants characterized by using alginic acid oligosaccharide.
アルギン酸の構成糖は、マンヌロン酸とグルロン酸であ
り、このような多糖を分解して得られるオリゴ糖は従来
報告されているオリゴガラクツロン酸やキシロオリゴ塘
とは全く化学構造式を異にしており、またオリゴ糖が植
物の根や地上部の生長を促進する作用があることも新規
な事実である。The constituent sugars of alginic acid are mannuronic acid and guluronic acid, and the oligosaccharide obtained by decomposing these polysaccharides has a completely different chemical structure from the previously reported oligogalacturonic acid and xylooligotang. It is also a new fact that oligosaccharides have the effect of promoting the growth of roots and above-ground parts of plants.
本発明においてアルギン酸オリゴ糖とは以下のように定
義される。アルギン酸、アルギン酸ナトリウムおよびア
ルギン酸を含有する昆布などの藻類、微生物起源の多糖
体などをアルギン酸リアーゼなどの酵素で分解するか、
または塩酸などの酸で加水分解して得られるオリゴ糖組
成物で、オリゴ糖の構成糖成分はグルロン酸およびマン
ヌロン酸が主成分である。その重合度が２〜２０までの
グルロン酸のみ、マンヌロン酸のみ、またはグルロン酸
とマンヌロン酸の組合せで構成されるオリゴＷ類および
グルロン酸、マンヌロン酸から成る組成物、さらにまた
この組成物をｐＨ１〜３゜１００〜１３０℃の条件下で
１５分〜１８０分加熱して得られる組成物をいう。In the present invention, alginate oligosaccharide is defined as follows. Alginic acid, sodium alginate, alginic acid-containing algae such as kelp, and microbial-derived polysaccharides are decomposed by enzymes such as alginate lyase, or
Alternatively, it is an oligosaccharide composition obtained by hydrolysis with an acid such as hydrochloric acid, and the constituent sugar components of the oligosaccharide are mainly guluronic acid and mannuronic acid. A composition consisting of oligo Ws consisting of only guluronic acid, only mannuronic acid, or a combination of guluronic acid and mannuronic acid, and guluronic acid and mannuronic acid having a polymerization degree of 2 to 20; ~3° refers to a composition obtained by heating for 15 to 180 minutes under conditions of 100 to 130°C.
このような組成物は、例えば以下のごとくして調製され
る。Such a composition is prepared, for example, as follows.
原料とするアルギン酸としては、市販のアルギン酸、ア
ルギン酸ソーダやアルギン酸を含有する昆布、カジノ、
レソニア、ドルベリアなどの海藻類およびシュードモナ
スなどの微生物の生産するアルギン酸様多ＩＪ！質など
アルギン酸を含有する原料は全て利用できる。Examples of alginic acid used as a raw material include commercially available alginic acid, sodium alginate, kelp containing alginic acid, casino,
Alginic acid-like multi-IJ produced by seaweeds such as Lawsonia and Dolberia and microorganisms such as Pseudomonas! All raw materials containing alginic acid, such as alginic acid, can be used.
アルギン酸を分解する手段としては、塩酸、硫酸などの
酸で加水分解する方法とアルギン酸リアーゼなどの酵素
で分解する手法が応用できる。酸で加水分解する場合は
、例えばアルギン酸ソーダ５部に１００部の水を加え、
アルギン酸を溶解した後、３部の濃塩酸を添加して９０
〜１００℃、２〜４時間加水分解を行った後、濾過し、
溶液を苛性ソーダで中和し、濃縮することによってアル
ギン酸オリゴ糖を調製することができる。また、アルギ
ン酸リアーゼで分解する場合は、アルギン酸ソーダ５部
に水を１００部加えてアルギン酸を溶解後、酵素の至適
作用ｐ ＨにｐＨを調整し、酵素をアルギン酸ソーダ１
ｇ当り１００〜４０００単位添加して、酵素の作用至適
温度で２４〜４８時間反応させることによってアルギン
酸オリゴ糖を調製することができる。As a means of decomposing alginic acid, a method of hydrolyzing it with an acid such as hydrochloric acid or sulfuric acid, and a method of decomposing it with an enzyme such as alginate lyase can be applied. When hydrolyzing with acid, for example, add 100 parts of water to 5 parts of sodium alginate,
After dissolving the alginic acid, add 3 parts of concentrated hydrochloric acid and
After hydrolysis at ~100°C for 2 to 4 hours, filtration,
Alginic acid oligosaccharides can be prepared by neutralizing the solution with caustic soda and concentrating it. When decomposing with alginate lyase, add 100 parts of water to 5 parts of sodium alginate to dissolve the alginic acid, adjust the pH to the optimum pH for the enzyme, and add 1 part of the enzyme to 1 part of sodium alginate.
Alginic acid oligosaccharide can be prepared by adding 100 to 4000 units per g and reacting for 24 to 48 hours at the optimum temperature for enzyme action.
アルギン酸リアーゼとしてアワビの消化管酵素（商品名
：アバロンアセトンパウダー、Ｍｅｒｋ社製）を用いる
場合は、至適作用ｐＨは７〜８、至適作用温度は２０〜
３５℃である。When using abalone gastrointestinal enzyme (trade name: Avalon Acetone Powder, manufactured by Merc) as alginate lyase, the optimum action pH is 7-8 and the optimum action temperature is 20-8.
The temperature is 35°C.
アルギン酸リアーゼの酵素活性はｐＨ７，０゜３０℃で
０．２％アルギン酸ソーダ溶液に酵素を作用させたとき
、３０分間に２３０部ｍの吸光度を０．０１上昇させる
酵素力を１単位として表示した。The enzymatic activity of alginate lyase is expressed as the enzyme power that increases the absorbance of 230 parts m by 0.01 in 30 minutes when the enzyme is applied to a 0.2% sodium alginate solution at pH 7.0° and 30°C. .
また、海藻類から直接オリゴ糖を得る場合は、例えば乾
燥昆布４０部に水を１３００部加え、ｐＨを１１とした
後、ホモゲナイザーで磨砕し、６０℃で３時間処理し、
その後ｐＨを５．５とした後、セルラーゼ（商品名：メ
イセラーゼ、明治製菓■製）を対固型分０．５％添加し
、４０℃で２０時間反応後、ｐＨを７．０とし、アルギ
ン酸リアーゼを固型分１ｇ当り１０００単位添加して３
０℃で４８時間反応させることにより昆布より直接アル
ギン酸オリゴ糖を製造することができる。In addition, when obtaining oligosaccharides directly from seaweed, for example, add 1,300 parts of water to 40 parts of dried kelp, adjust the pH to 11, grind with a homogenizer, and treat at 60°C for 3 hours.
After that, the pH was adjusted to 5.5, and 0.5% of cellulase (trade name: Meicelase, manufactured by Meiji Seika) was added to the solid content, and after reacting at 40°C for 20 hours, the pH was adjusted to 7.0, and alginic acid 3 by adding 1000 units of lyase per 1g of solid content.
Alginic acid oligosaccharides can be produced directly from kelp by reacting at 0°C for 48 hours.
このようにして得られたアルギン酸オリゴ糖は、その構
成糖成分はマンヌロン酸とグルロンｗが主成分であり、
重合度が２〜２０までのグルロン酸のみ、マンヌロン酸
のみ、または、グルロン酸とマンヌロン酸の組合せで構
成されるオリゴ糖類およびグルロン酸、マンヌロン酸の
組成物である。The alginic acid oligosaccharide obtained in this way has mannuronic acid and guluron w as its main constituent sugar components,
It is a composition of oligosaccharides, guluronic acid, and mannuronic acid having a degree of polymerization of 2 to 20 and composed of only guluronic acid, only mannuronic acid, or a combination of guluronic acid and mannuronic acid.
上記のごとくして得られた分解物中のアルギン酸オリゴ
糖の含有量は、分解原料によって種々の値をとるが、例
えばアルギン酸ナトリウムを原料とした酵素分解法で調
製した場合は、固型物中４０〜１００％に達する。また
、昆布などの海藻類を原料とした場合は固型物中１０〜
２５％である。The content of alginate oligosaccharide in the decomposition product obtained as described above takes various values depending on the decomposition raw material, but for example, when it is prepared by an enzymatic decomposition method using sodium alginate as the raw material, the content of alginate oligosaccharide in the solid material is It reaches 40-100%. In addition, when seaweed such as kelp is used as raw material, 10 to 10
It is 25%.
このようにして得られたアルギン酸オリゴ糖は、種子な
どに塗布したり、０．２５〜０．０００２５％の水溶液
として土壌中に添加したり、葉面散布を行ったり、さら
にまた養液栽培用液体肥料中に添加混合するなどして植
物に施用すると、植物の根および地上部の生長を促進し
、その結果収［量が向上するが、このような作用は上記
のごとくして得られた組成物をｐＨ１〜３．好ましくは
ｐＨ２，０〜３．０で、１００〜１２０℃の温度条件下
で１５〜１８０分間加熱処理を行うことによりさらに促
進されることが明らかとなった。なお、アルギン酸また
はアルギン酸ソーダの未分解物は、例えば後記実施例１
に示すように植物の生長促進作用は全く認められなかっ
た。The alginic acid oligosaccharide thus obtained can be applied to seeds, etc., added to soil as a 0.25 to 0.00025% aqueous solution, sprayed on leaves, and used for hydroponic cultivation. When applied to plants by adding it to a liquid fertilizer, it promotes the growth of the roots and above-ground parts of the plant, resulting in an increase in yield. The composition has a pH of 1 to 3. It has been found that heat treatment is further promoted, preferably at a pH of 2.0 to 3.0, at a temperature of 100 to 120°C for 15 to 180 minutes. In addition, undecomposed products of alginic acid or sodium alginate can be used, for example, in Example 1 below.
As shown in Figure 2, no plant growth promoting effect was observed.
次に、本発明を実施例により詳しく説明する。 Next, the present invention will be explained in detail with reference to examples.
実施例１
アルギン酸にアルギン酸リアーゼ（アバロンアセトンパ
ウダー）と４０００ ｕ／ｇアルギン酸の割合で添加し
、ｐＨ７，０，４０℃で４８時間反応させてオリゴ糖を
調製した後、ｐＨを３．０に調整し、１２０℃で２時間
加熱処理を行なった。Example 1 Alginate lyase (Avalon Acetone Powder) and alginic acid were added to alginic acid at a ratio of 4000 u/g alginic acid, and reacted at pH 7.0 and 40°C for 48 hours to prepare oligosaccharides, and then the pH was adjusted to 3.0. Then, heat treatment was performed at 120°C for 2 hours.
加熱処理後、反応液のｐＨを７．０に中和し、加かいわ
れ大根の種子３６粒を合成樹脂製のウールマットをセッ
トしたガラス容器に播種し、水道水７０ｒａｌを添加し
て２３℃で６日間栽培した（４日間は暗所で、続く２日
間は５ｏｏｏルツクスの照射条件下で栽培）。アルギン
酸オリゴ糖は対水道水当り２．５〜０．００００２５％
の割合で添加した。結果を表１に示す。アルギン酸オリ
ゴ糖はいずれの濃度条件下でもアルギン酸オリゴ糖無添
加の対照区に対して茎葉長、根長共にその生長を促進す
るが、ｐＨ３，０，１２０℃、２時間加熱区ではその効
果が増強される傾向にあった。After the heat treatment, the pH of the reaction solution was neutralized to 7.0, 36 seeds of Kakaiware radish were sown in a glass container set with a synthetic resin wool mat, and 70 ral of tap water was added, and the mixture was incubated at 23°C. It was cultivated for 6 days (4 days in the dark and the following 2 days under irradiation conditions of 500 lux). Alginate oligosaccharide is 2.5-0.000025% based on tap water
It was added at a ratio of The results are shown in Table 1. Alginate oligosaccharide promotes the growth of both shoot and root length compared to the control group without alginate oligosaccharide under any concentration condition, but the effect is enhanced at pH 3, 0, and 120 ° C. for 2 hours heating. There was a tendency to
なお、表１における数値は、アルギン酸オリゴ糖などを
無添加の条件下で栽培した茎葉長（ｅ、）または根長（
ｃｍ）の平均値を１００とした場合の指数で示した。（
ｎ＝３６）
実施例２
号０．１５％、同２号０．１％を含む液肥中に浸漬した
後、２３℃、５０００ルツクスの条件下で１０日間栽培
して発芽・育苗し、その後水耕栽培装置中に定植し、８
０００ルツクス、２３〜２４℃の条件下で２．５ケ月栽
培した。実験区は以下の通りである。The values in Table 1 are the stem and leaf lengths (e,) or root lengths (e,
It is expressed as an index when the average value of cm) is set as 100. (
n = 36) Example 2 After soaking in liquid fertilizer containing 0.15% of No. 2 and 0.1% of No. 2, cultivation was carried out for 10 days at 23°C and 5000 lux to germinate and raise seedlings, and then watered. Planted in cultivation equipment, 8
000 lux and 23 to 24°C for 2.5 months. The experimental areas are as follows.
対照区ニアルギン酸オリゴ糖無添加の液肥で育苗した後
、引続いて無添加液肥で栽培。Control group Seedlings were raised with liquid fertilizer without addition of nialginic acid oligosaccharide, and then cultivated with liquid fertilizer without additives.
アルギン酸オリゴ糖添加区ニアルギン酸オリゴｔＪ！０
．０２５％を添加した液肥で育苗した後、引続いてアル
ギン酸オリゴ糖
０．０２５％を含む液肥で栽培。Alginic acid oligosaccharide addition group Nialginic acid oligo tJ! 0
．． After raising seedlings with liquid fertilizer containing 0.025%, they were subsequently cultivated with liquid fertilizer containing 0.025% oligosaccharide alginate.
なお、アルギン酸オリゴ糖は、アルギン酸ソーダ５％溶
液（ｐＨ７，０）にアルギン酸リアーゼ３．０とし、１
２０℃で２時間加熱処理を行い、冷却後ｐＨ７，０に中
和したものを用いた。In addition, the alginate oligosaccharide was prepared by adding alginate lyase 3.0 to a 5% sodium alginate solution (pH 7.0) and adding 1
The mixture was heat-treated at 20° C. for 2 hours, cooled, and then neutralized to pH 7.0.
試験結果を表２に示す。アルギン酸オリゴ糖の添加によ
りミツバの増収が認められた。The test results are shown in Table 2. An increase in the yield of Mitsuba was observed with the addition of alginate oligosaccharide.
表２
（ｎ＝２０）
実施例３
アルギン酸オリゴｔＦ０．２５％、アルギン酸ナトリウ
ム０．７５％を含む水溶液１重量部をかいわれ大根の種
子１重量部に対し噴霧し、４０〜５０℃の気流中で乾燥
し、アルギン酸オリゴ糖を種子コートした種子を調製し
た。Table 2 (n=20) Example 3 1 part by weight of an aqueous solution containing 0.25% alginate oligo tF and 0.75% sodium alginate was sprayed onto 1 part by weight of radish seeds in an air stream at 40 to 50°C. Seeds were prepared by drying and seed-coating with alginate oligosaccharides.
このようにして得られたアルギン酸オリゴ糖コート種子
５０粒を合成樹脂製ウールマントを設置したガラス容器
中に播種し、水道水７０Ｉｌ１１を添加して２３℃で４
日間は暗所で、続く２日間はし、同一栽培条件下で栽培
した。結果を表３に示す。50 alginic acid oligosaccharide-coated seeds obtained in this way were sown in a glass container equipped with a synthetic resin wool mantle, and 70 liters of tap water was added thereto for 4 hours at 23°C.
The seeds were grown in the dark for one day and then for the next two days under the same cultivation conditions. The results are shown in Table 3.
表３
種子１ｇ当り２．５■塗付したコート種子を用いると、
茎葉長で１１８％、根長で１６４％の伸長が認められた
。Table 3 When using coated seeds coated with 2.5 μg per gram of seeds,
An elongation of 118% in stem and leaf length and 164% in root length was observed.
実施例４
黒土９ ｋｇを１７ｃｍＸ ６０ｃｍＸ ｌ ５ｃｍの
ポットに添加し、小松菜（品種名、みすぎ小松菜）の種
子４０粒を播種し、６月１５日から７月４日まで自然条
件下で栽培した。実験区は以下の通りである。Example 4 9 kg of black soil was added to a 17 cm x 60 cm x l 5 cm pot, 40 seeds of Komatsuna (cultivar name: Misugi Komatsuna) were sown, and the pot was cultivated under natural conditions from June 15th to July 4th. . The experimental areas are as follows.
対照区ニアルギン酸オリゴ糖無添加
添加区；アルギン酸オリゴ糖２２ ｇを３．６１の水溶
液とし、これを黒土に全量添加
して黒土に対し、アルギン酸オリゴ糖
０．２５％を添加した土壌となし、栽培を行った。Control area: No addition of alginic acid oligosaccharide; 22 g of alginic acid oligosaccharide was made into a 3.61 aqueous solution, and the entire amount was added to black soil to create soil with 0.25% alginic acid oligosaccharide added to the black soil, cultivated.
なお、アルギン酸オリゴ糖は実施例２に記載の方法で調
製したものを用いた。結果を表４に示す。Note that the alginate oligosaccharide prepared by the method described in Example 2 was used. The results are shown in Table 4.
表４
（ｎ＝４０）
アルギン酸オリゴ糖を土壌中に添加することにより、２
０％の増収が認められた。Table 4 (n=40) By adding alginate oligosaccharide to soil, 2
A 0% increase in sales was observed.
実施例５
トウモロコシ種子を３３ｍ！当り３６粒播種し、自然条
件下で３．５ケ月間栽培を行った。実験区は以下の通り
である。Example 5 33m of corn seeds! 36 seeds were sown per seed and cultivated for 3.5 months under natural conditions. The experimental areas are as follows.
対照区ニアルギン酸オリゴ糖無処理区
添加区：発芽後茎葉長が８〜１２ｃｍになった時点でア
ルギン酸オリゴ糖６ｇを０．０５％の水溶液として根の
周辺部に施用し
た。さらに、１．５ケ月後にアルギン酸オリゴ＄７！６
ｇを同様に追加施用した。Control group, nialginate oligosaccharide untreated group, added group: After germination, when the length of the leaves reached 8 to 12 cm, 6 g of alginate oligosaccharide was applied as a 0.05% aqueous solution to the surrounding area of the roots. Furthermore, after 1.5 months, alginate oligo $7!6
g was additionally applied in the same manner.
なお、アルギン酸オリゴ糖は、実施例２に記載の方法に
従って調製したものを用いた。結果を表５に示す。Note that the alginate oligosaccharide prepared according to the method described in Example 2 was used. The results are shown in Table 5.
表５
トウモロコシの収量はアルギン酸オリゴ糖の施用によっ
て２６％の増収が認められた。Table 5 A 26% increase in corn yield was observed by applying alginic acid oligosaccharide.
実施例６
育苗用トレーにキュウリの種子（品種名、貴婦人）１０
０粒を播種し、２０〜２３℃で１週間栽培した後、三寸
ポットに定植し、更に二週間育苗した。このようにして
得られた苗を土壌中に８０備間隔で定植し、自然条件下
で３ケ月間栽培した。Example 6 10 cucumber seeds (variety name, Lady) in a seedling tray
After sowing 0 seeds and cultivating them at 20 to 23°C for one week, they were planted in three-inch pots and the seedlings were raised for another two weeks. The seedlings thus obtained were planted in soil at 80-meter intervals and cultivated under natural conditions for 3 months.
実験区は以下の通りである。The experimental areas are as follows.
対照区ニアルギン酸オリゴ糖無処理
添加区：ニオポット定植後、３日目にアルギン酸オリゴ
糖をポット当り２５■の割合
で５０ＩＩ１１の水に溶解して施用した。Control area: Non-treated and added nialginic acid oligosaccharide area: On the 3rd day after planting in Niopot, alginic acid oligosaccharide was dissolved in 50II and 11 parts of water and applied at a rate of 25 ml per pot.
また、定植後３週間目に５０■を５００ＩＩ１１の水に
溶解し、追加施用した。Further, 3 weeks after planting, 50 ml of the solution was dissolved in 500 II of water and an additional application was made.
なお、アルギン酸オリゴ糖は実施例２と同様の方法で調
製した。結果を表６に示す。Note that alginic acid oligosaccharide was prepared in the same manner as in Example 2. The results are shown in Table 6.
表６
アルギン酸オリゴ糖の施用によりキュウリの収穫量は１
９％向上した。Table 6 Cucumber yield increased by 1 by applying alginate oligosaccharide.
It improved by 9%.
本発明にしたがってアルギン酸オリゴ糖を用いることに
より、有用植物の生育を促進し、かつその収穫量を増す
ことができる。By using alginate oligosaccharides according to the present invention, it is possible to promote the growth of useful plants and increase their yield.
Claims (1)
の栽培方法。A method for cultivating useful plants, characterized by using alginate oligosaccharide.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61245400A JPS63101302A (en) | 1986-10-17 | 1986-10-17 | Culture of plant |
| CA000549522A CA1332880C (en) | 1986-10-17 | 1987-10-16 | Plant cultivation method |
| FR8714303A FR2605185B1 (en) | 1986-10-17 | 1987-10-16 | PLANT CULTIVATION PROCESS |
| CN87107747A CN1020842C (en) | 1986-10-17 | 1987-10-17 | Plant cultivation method |
| DE3735365A DE3735365C2 (en) | 1986-10-17 | 1987-10-19 | Method for accelerating plant growth |
| US07/571,003 US5588254A (en) | 1986-10-17 | 1990-08-22 | Plant cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61245400A JPS63101302A (en) | 1986-10-17 | 1986-10-17 | Culture of plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63101302A true JPS63101302A (en) | 1988-05-06 |
| JPH0433408B2 JPH0433408B2 (en) | 1992-06-03 |
Family
ID=17133089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61245400A Granted JPS63101302A (en) | 1986-10-17 | 1986-10-17 | Culture of plant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63101302A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001040315A1 (en) * | 1999-11-30 | 2001-06-07 | Dalian Yaweite Biology Engineering Co., Ltd. | The alginate having low molecular weight, methods of manufacturing it and its use |
| JP2003503322A (en) * | 1999-06-25 | 2003-01-28 | サントル・ナショナル・ドゥ・ラ・ルシェルシュ・シャンティフィク | Use of glycuronic polysaccharides and oligosaccharides as phytosanitary products and / or fertilizers |
| JP2019202993A (en) * | 2018-05-07 | 2019-11-28 | ソシエテ・デチュード・エ・デクスプロワタシオン・ダルグ・エ・プロデュイ・マリティム（セテクサム）Ｓｏｃｉｅｔｅ Ｄ’Ｅｔｕｄｅ Ｅｔ Ｄ’Ｅｘｐｌｏｉｔａｔｉｏｎ Ｄ’Ａｌｇｕｅｓ Ｅｔ Ｐｒｏｄｕｉｔｓ Ｍａｒｉｔｉｍｅｓ （Ｓｅｔｅｘａｍ） | Use of extract or extract fraction of agar type red algae as plant protective elicitor/stimulus substance, and application of said extract or extract fraction |
-
1986
- 1986-10-17 JP JP61245400A patent/JPS63101302A/en active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003503322A (en) * | 1999-06-25 | 2003-01-28 | サントル・ナショナル・ドゥ・ラ・ルシェルシュ・シャンティフィク | Use of glycuronic polysaccharides and oligosaccharides as phytosanitary products and / or fertilizers |
| JP4739622B2 (en) * | 1999-06-25 | 2011-08-03 | サントル・ナショナル・ドゥ・ラ・ルシェルシュ・シャンティフィク | Use of glycuronic polysaccharides and oligosaccharides as plant hygiene and / or fertilizers |
| WO2001040315A1 (en) * | 1999-11-30 | 2001-06-07 | Dalian Yaweite Biology Engineering Co., Ltd. | The alginate having low molecular weight, methods of manufacturing it and its use |
| JP2019202993A (en) * | 2018-05-07 | 2019-11-28 | ソシエテ・デチュード・エ・デクスプロワタシオン・ダルグ・エ・プロデュイ・マリティム（セテクサム）Ｓｏｃｉｅｔｅ Ｄ’Ｅｔｕｄｅ Ｅｔ Ｄ’Ｅｘｐｌｏｉｔａｔｉｏｎ Ｄ’Ａｌｇｕｅｓ Ｅｔ Ｐｒｏｄｕｉｔｓ Ｍａｒｉｔｉｍｅｓ （Ｓｅｔｅｘａｍ） | Use of extract or extract fraction of agar type red algae as plant protective elicitor/stimulus substance, and application of said extract or extract fraction |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0433408B2 (en) | 1992-06-03 |
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