CN109042666A - A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate - Google Patents
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate Download PDFInfo
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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Abstract
The invention discloses a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate, it includes the component of following concentration: 20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 100 mg/L of proline, 2 ml/L of Tween-80, and above-mentioned foliage-spray liquid further includes the hydroxyisoxazole that concentration is 400-1000 mg/L.Foliage-spray liquid of the invention can effectively enhance the resistance of test tube seedling, so that sugarcane test tube seedling can effectively improve in high temperature and humidity and the disease survival rate planted in severe environments that causes harm, cultivation matrix raw material sources have been further expanded, cultivation matrix cost of manufacture is reduced.
Description
Technical field
The invention belongs to the rooting of vitro seedling technologies within the scope of plant tissue culture technique, and in particular to a kind of raising sugarcane
The technology of rooting of vitro seedling rate and survival rate.
Background technique
Plant Tissue Breeding will generally pass through four-stage, i.e. Initial culture (or establishing sterile culture system), subculture increases
Grow culture, culture of rootage and acclimatization and transplants.Currently, in the sugarcane test tube seedling fast breeding technology for producing upper large-scale application
It include the above four-stage.However this traditional sugarcane test tube seedling production process complexity, and it is high to culture environment condition, it causes
Keep the cost of sugarcane tissue culture the factorial production test tube seedling high, is increasingly not suitable with sugarcane production requirement, limits
The plantation and popularization of sugarcane health seedling.Thus, simplify sugarcane test tube seedling production routine, reduce production cost, is to solve to restrict
The most effective approach of this problem is promoted in sugarcane health seedling large-scale production.
Studies have shown that the cost of taking root of test tube seedling accounts for entire test tube seedling production cost in plant tissue culture course
35%-75%, therefore simplified rooting of vitro seedling technology can make plant test tube seedling production cost sharp fall.Out Test-tube rooting of Test-tube Seedlings
Technology is successful one advanced tissue culture rooting technique of research in recent years, is the important composition that plant test tube seedling simplifies production technology
Part.Advance in non-tube rootage technology is directly to cultivate unrooted test tube seedling to take root in greenhouse nursery, by traditional life
Root and two stages of domestication combine, and eliminate the traditional program of the in vitro rooting of unrooted test tube seedling, are taken root generation with autotrophy
It takes root for traditional heterotrophism, shortens growing-seedling period, save production cost.Meanwhile the development of plant tissue culture technique is very fast
Speed both at home and abroad grinds large quantities of plant Advance in non-tube rootage technology such as babysbreath, Siraitia grosvenorii, tree peony, sugarcane, strawberry
Study carefully, and obtains success.
Test tube seedling foliage-spray liquid and the cultivation matrix of test tube seedling are all the key factors for influencing test tube seedling survival rate, well
Foliage-spray formula of liquid be aided with suitable cultivation matrix again and can effectively improve the resistance and survival rate of test tube seedling, and do not conform to
Suitable cultivation matrix can but reduce the survival rate of test tube seedling.As the Chinese patent of Patent No. 201510273074.7 discloses one
It kind improves the take root growth of efficiency of sugarcane test tube seedling photoautotrophy and promotes liquid and method, use root-inducing powder, proline and tween-
80, which prepare growth, promotes liquid to spray in the hardening stage to test tube seedling, and sugarcane test tube seedling survival rate can be improved, delay blade
The ability of aging and the enhancing degeneration-resistant border of test tube seedling.
Summary of the invention
Needle in view of the above deficiencies, the present invention to promote the growth regulator taken root of autotrophy be further improved with it is complete
It is kind, it proposes a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate, can effectively enhance the resistance of test tube seedling, so that examination
Guan Miao can effectively improve in high temperature and humidity and the disease survival rate planted in severe environments that causes harm, and further expand cultivation
Base starting material source is trained, cultivation matrix cost of manufacture is reduced.
The present invention is achieved by the following technical scheme:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate comprising the component of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 100 mg/L of proline, 2 ml/L of Tween-80.
Preferably, the foliage-spray liquid of a kind of raising sugarcane test tube seedling survival rate, further includes following concentration
Component: hydroxyisoxazole 400-1000 mg/L.
Preferably, the foliage-spray liquid of a kind of raising sugarcane test tube seedling survival rate, by the component of following concentration
Composition: 20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 600 mg/L of hydroxyisoxazole, 100 mg/ of proline
L, 2 ml/L of Tween-80.
The processing of the foliage-spray liquid application method and test tube seedling of a kind of raising sugarcane test tube seedling survival rate, kind
Plant, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
The processing of above-mentioned foliage-spray liquid application method and test tube seedling, plantation, in management method, in step (4) in
The pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1.
The processing of above-mentioned foliage-spray liquid application method and test tube seedling, plantation, in management method, in step (3) in
The bright shield is the fungicide product of Syngenta crop protection Co., Ltd production.
The technical program has the advantages that compared with prior art
(1) foliage-spray liquid of the invention effectively enhances the resistance of test tube seedling, causes harm sternly in high temperature and humidity and pathogenic microorganism
It is planted in the environment of weight, the survival rate of test tube seedling can effectively improve, and further expanded cultivation matrix raw material sources, opened up
Test tube plantation environmental field has been opened up, cultivation matrix cost of manufacture is reduced.
(2) indolebutyric acid and methyl α-naphthyl acetate are used in mixed way in the present invention, and their concentration is controlled as 20
Mg/L and 10 mg/L, indolebutyric acid or methyl α-naphthyl acetate compared to the prior art are used alone added concentration and are greatly decreased,
Cost of material of the invention is reduced, and spray liquid of the invention still can effectively facilitate the growth of test tube seedling, improve it and deposit
Motility rate.
(3) hydroxyisoxazole added in the present invention is a kind of wide-spectrum bactericide, can be fast in 24 hours after being absorbed by test tube seedling
Speed is transmitted to each position of test tube seedling, can enhance sugarcane test tube seedling to Fusarium (Fusarium), marrow Achorion (Pithium)
The resistance of equal soil diseases, and hydroxyisoxazole generates two kinds of glucosides in plant intracellular metabolite, is improved the effect of test tube seedling physiological activity
Fruit, so as to promote plant strain growth, the tiller of root, the increase of root hair and the activity of root are improved, and test tube seedling resistance are improved, into one
Step improves transplanting survival rate of the test tube seedling in different planting environments, especially with the pathogenic microorganism of pond sludge class cultivation matrix
More planting environment.
(4) it also added sucrose in the present invention, sucrose is the energy source of test tube seedling growth, and addition sucrose can promote test tube
Seedling healthy growth and the degeneration-resistant border ability for improving test tube seedling.
(5) foliage-spray liquid consumption of raw materials of the invention is few, low in cost, prepare simple, easy to use, is conducive to extensive
Production and application.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as limitation of the present invention.Following implementation
The specific experiment condition and method being not specified in example, used technological means are usually well-known to those skilled in the art normal
Rule means.
Embodiment 1:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate, is grouped as by the group of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 400 mg/L of hydroxyisoxazole, 100 mg/ of proline
L, 2 ml/L of Tween-80.
The foliage-spray liquid application method and the processing of test tube seedling, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Embodiment 2:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate, is grouped as by the group of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 600 mg/L of hydroxyisoxazole, 100 mg/ of proline
L, 2 ml/L of Tween-80.
The foliage-spray liquid application method and the processing of test tube seedling, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Embodiment 3:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate, is grouped as by the group of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 800 mg/L of hydroxyisoxazole, 100 mg/ of proline
L, 2 ml/L of Tween-80.
The foliage-spray liquid application method and the processing of test tube seedling, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Embodiment 4:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate, is grouped as by the group of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 1000 mg/L of hydroxyisoxazole, 100 mg/ of proline
L, 2 ml/L of Tween-80.
The foliage-spray liquid application method and the processing of test tube seedling, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Embodiment 5:
A kind of foliage-spray liquid improving sugarcane test tube seedling survival rate, is grouped as by the group of following concentration:
20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 100 mg/L of proline, 2 ml/L of Tween-80.
The foliage-spray liquid application method and the processing of test tube seedling, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Comparative example 1:
It is a kind of improve sugarcane test tube seedling photoautotrophy take root efficiency growth promote liquid, the formula are as follows:
200 mg/L of ABT2 root-inducing powder, 100 mg/L of proline, 2 ml/L of Tween-80.
The take root growth of efficiency of the raising sugarcane test tube seedling photoautotrophy promotes the place of liquid application method and test tube seedling
Reason, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays foliage-spray liquid: 200 mg/L of ABT2 root-inducing powder, 100 mg/L of proline, Tween-80
2 ml/L, then at 20~30 DEG C of temperature, humidity 65~80%, under 2000~10000 lux of intensity of illumination, hardening 24-48 is small
When；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: by the test tube seedling after segmentation cleaning with liquor potassic permanganate soaking disinfection 3~6 minutes of 0.2~0.3%；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water, the pond sludge and fresh river sand mixture that the cultivation matrix is volume ratio 1:1；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive adjusts seedling cultivation greenhouse environment: in 12 days after plantation, chamber humidity control 80% or more, intensity of illumination 2000~
10000 lux；After plantation 12 days, chamber humidity control is 70% or more, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
Sugared No. 49 sugar cane breeds in selection osmanthus, the shoot proliferation culture of progress sugarcane test tube seedling, subculture multiplication medium MS,
1 mg/L of 6- benzyl aminoadenine, 0.01 mg/L of methyl α-naphthyl acetate, 20 g/L of sucrose, according to embodiment after squamous subculture 12~15 days
Method described in 1-5 and comparative example 1 handles 250 bottles of sugarcane test tube seedling, plants 5 basins, and planting pot specification is 9 holes × 6 holes, plantation 30
Survival rate is investigated after it, the result is shown in tables 1:
Table 1
As seen from the above table, compared with promoting liquid phase with growth commonly used in the prior art, foliage-spray liquid of the invention can be substantially
Improve the survival rate of sugarcane test tube seedling.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (5)
1. a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate, it is characterised in that: it includes the component of following concentration: Yin
20 mg/L of diindyl butyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 100 mg/L of proline, 2 ml/L of Tween-80.
2. a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate according to claim 1, it is characterised in that: it is also
Component including following concentration: hydroxyisoxazole 400-1000 mg/L.
3. it is according to claim 2 it is a kind of improve sugarcane test tube seedling survival rate foliage-spray liquid, it is characterised in that: its by
The group of following concentration is grouped as: 20 mg/L of indolebutyric acid, 10 mg/L of methyl α-naphthyl acetate, sucrose 20g/L, 600 mg/L of hydroxyisoxazole
, 100 mg/L of proline, 2 ml/L of Tween-80.
4. a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate according to claim 1, it is characterised in that: described
The processing of the application method and test tube seedling of foliage-spray liquid, plantation, management method are as follows:
(1) hardening: will complete shoot proliferation cultivation cycle, and the unrooted sugarcane seedling bottle containing subculture multiplication medium be put in it is shady and cool,
Lee opens bottle cap, sprays the foliage-spray liquid of above-mentioned formula, then at 20~30 DEG C of temperature, humidity 65~80%, and illumination
Under 2000~10000 lux of intensity, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, the highly test tube seedling single plant greater than 1 cm is divided, and height is small
Involve body segmentation in a criminal case by 2-3 in the test tube seedling of 1 cm, cleans culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated 8~10 minutes；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, it is fixed to be watered with
Root water；
(5) it takes root cultivating and managing: the planting pot with test tube seedling being moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, covers screening
Screened postive, adjust seedling cultivation greenhouse environment: chamber humidity control is 60~80%, 2000~10000 lux of intensity of illumination；When 90% it is sweet
After sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of water drenching in 30 minutes 30 seconds, until test tube
Seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and conventional sugarcane test tube seedling method for culturing seedlings phase
Together.
5. a kind of foliage-spray liquid for improving sugarcane test tube seedling survival rate according to claim 4, it is characterised in that: step
(4) pond sludge and fresh river sand mixture that the cultivation matrix described in is volume ratio 1:1.
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| CN109553480A (en) * | 2019-01-14 | 2019-04-02 | 广西壮族自治区农业科学院 | A kind of sugarcane production-increasing that is precisely sprayed based on unmanned plane increases sugared growth promoter and its method of administration |
| CN110915598A (en) * | 2019-12-19 | 2020-03-27 | 广西壮族自治区农业科学院 | Leaf surface spraying liquid, method and illumination device for photoautotrophic rooting of sugarcane test-tube plantlets |
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| CN109553480A (en) * | 2019-01-14 | 2019-04-02 | 广西壮族自治区农业科学院 | A kind of sugarcane production-increasing that is precisely sprayed based on unmanned plane increases sugared growth promoter and its method of administration |
| CN110915598A (en) * | 2019-12-19 | 2020-03-27 | 广西壮族自治区农业科学院 | Leaf surface spraying liquid, method and illumination device for photoautotrophic rooting of sugarcane test-tube plantlets |
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