JPS6260061B2 - - Google Patents
Info
- Publication number
- JPS6260061B2 JPS6260061B2 JP59195932A JP19593284A JPS6260061B2 JP S6260061 B2 JPS6260061 B2 JP S6260061B2 JP 59195932 A JP59195932 A JP 59195932A JP 19593284 A JP19593284 A JP 19593284A JP S6260061 B2 JPS6260061 B2 JP S6260061B2
- Authority
- JP
- Japan
- Prior art keywords
- tray
- section
- signal
- cell
- pipette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000243 solution Substances 0.000 claims description 69
- 238000001514 detection method Methods 0.000 claims description 32
- 238000010790 dilution Methods 0.000 claims description 28
- 239000012895 dilution Substances 0.000 claims description 28
- 238000002955 isolation Methods 0.000 claims description 21
- 238000003556 assay Methods 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 17
- 239000006228 supernatant Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 11
- 239000003085 diluting agent Substances 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 147
- 230000032258 transport Effects 0.000 description 16
- 210000000628 antibody-producing cell Anatomy 0.000 description 11
- 238000005259 measurement Methods 0.000 description 9
- 230000007246 mechanism Effects 0.000 description 8
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- 239000012228 culture supernatant Substances 0.000 description 6
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- 238000012545 processing Methods 0.000 description 6
- 239000013076 target substance Substances 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 230000007723 transport mechanism Effects 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- MUXFZBHBYYYLTH-UHFFFAOYSA-N Zaltoprofen Chemical compound O=C1CC2=CC(C(C(O)=O)C)=CC=C2SC2=CC=CC=C21 MUXFZBHBYYYLTH-UHFFFAOYSA-N 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19593284A JPS6174570A (ja) | 1984-09-18 | 1984-09-18 | 細胞選別装置 |
EP85904852A EP0195088B1 (de) | 1984-09-18 | 1985-09-18 | Vorrichtung zum trennen von zellen |
DE8585904852T DE3586892T2 (de) | 1984-09-18 | 1985-09-18 | Vorrichtung zum trennen von zellen. |
PCT/JP1985/000519 WO1986001824A1 (fr) | 1984-09-18 | 1985-09-18 | Installation de triage de cellules |
US07/437,287 US5106584A (en) | 1984-09-18 | 1989-11-16 | Cell selecting apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19593284A JPS6174570A (ja) | 1984-09-18 | 1984-09-18 | 細胞選別装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6174570A JPS6174570A (ja) | 1986-04-16 |
JPS6260061B2 true JPS6260061B2 (de) | 1987-12-14 |
Family
ID=16349368
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP19593284A Granted JPS6174570A (ja) | 1984-09-18 | 1984-09-18 | 細胞選別装置 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6174570A (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10344284A1 (de) * | 2003-09-24 | 2005-05-04 | Keyneurotek Ag | Vorrichtung und Verfahren zur automatisierten Durchführung von Laborarbeitsschritten |
JPWO2007004385A1 (ja) * | 2005-07-05 | 2009-01-22 | 株式会社ニコン | 培養装置 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5754838A (en) * | 1980-09-18 | 1982-04-01 | Omron Tateisi Electronics Co | Automatic specimen feeder |
JPS5799189A (en) * | 1980-12-10 | 1982-06-19 | Becton Dickinson Co | Analysing and estimating apparatus for testing organic substance |
-
1984
- 1984-09-18 JP JP19593284A patent/JPS6174570A/ja active Granted
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5754838A (en) * | 1980-09-18 | 1982-04-01 | Omron Tateisi Electronics Co | Automatic specimen feeder |
JPS5799189A (en) * | 1980-12-10 | 1982-06-19 | Becton Dickinson Co | Analysing and estimating apparatus for testing organic substance |
Also Published As
Publication number | Publication date |
---|---|
JPS6174570A (ja) | 1986-04-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EXPY | Cancellation because of completion of term |