JPS6244616B2 - - Google Patents

Info

Publication number: JPS6244616B2
Authority: JP; Japan
Prior art keywords: bilirubin; liquid; mordant; interactive; fluorescence
Prior art date: 1979-08-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP54100798A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5524694A (en

Inventor

Uingu U Tai

Sanfuoodo Sariuan Sheriru

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Eastman Kodak Co

Original Assignee

Eastman Kodak Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1979-08-09

Filing date

1979-08-09

Publication date

1987-09-21

1979-08-09 Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co

1979-08-09 Priority to JP10079879A priority Critical patent/JPS5524694A/ja

1980-02-21 Publication of JPS5524694A publication Critical patent/JPS5524694A/ja

1987-09-21 Publication of JPS6244616B2 publication Critical patent/JPS6244616B2/ja

Status Granted legal-status Critical Current

Links

BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 306
230000002452 interceptive effect Effects 0.000 claims description 61
238000000034 method Methods 0.000 claims description 49
239000007788 liquid Substances 0.000 claims description 48
239000003153 chemical reaction reagent Substances 0.000 claims description 27
238000004458 analytical method Methods 0.000 claims description 25
230000005855 radiation Effects 0.000 claims description 20
238000012360 testing method Methods 0.000 claims description 16
238000005259 measurement Methods 0.000 claims description 14
238000012921 fluorescence analysis Methods 0.000 claims description 13
230000003213 activating effect Effects 0.000 claims description 12
239000011159 matrix material Substances 0.000 claims description 11
229920000642 polymer Polymers 0.000 claims description 10
239000006185 dispersion Substances 0.000 claims description 9
102000004169 proteins and genes Human genes 0.000 claims description 7
108090000623 proteins and genes Proteins 0.000 claims description 7
210000002966 serum Anatomy 0.000 claims description 7
125000002091 cationic group Chemical group 0.000 claims description 5
239000012530 fluid Substances 0.000 claims description 5
230000002209 hydrophobic effect Effects 0.000 claims description 5
-1 vinylbenzyl Chemical group 0.000 claims description 4
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 2
MYRTYDVEIRVNKP-UHFFFAOYSA-N divinylbenzene Substances C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 claims description 2
239000011248 coating agent Substances 0.000 claims 1
238000000576 coating method Methods 0.000 claims 1
239000010410 layer Substances 0.000 description 51
239000000126 substance Substances 0.000 description 15
239000000203 mixture Substances 0.000 description 13
230000003993 interaction Effects 0.000 description 10
239000000463 material Substances 0.000 description 9
102000009027 Albumins Human genes 0.000 description 6
108010088751 Albumins Proteins 0.000 description 6
239000000243 solution Substances 0.000 description 6
125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 5
238000005558 fluorometry Methods 0.000 description 5
239000004094 surface-active agent Substances 0.000 description 5
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
230000005284 excitation Effects 0.000 description 4
239000002657 fibrous material Substances 0.000 description 4
230000001965 increasing effect Effects 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
102000053187 Glucuronidase Human genes 0.000 description 3
108010060309 Glucuronidase Proteins 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
238000010521 absorption reaction Methods 0.000 description 3
239000007864 aqueous solution Substances 0.000 description 3
230000008901 benefit Effects 0.000 description 3
238000004737 colorimetric analysis Methods 0.000 description 3
238000011534 incubation Methods 0.000 description 3
230000008569 process Effects 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
238000007704 wet chemistry method Methods 0.000 description 3
PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
238000008050 Total Bilirubin Reagent Methods 0.000 description 2
239000012491 analyte Substances 0.000 description 2
238000003556 assay Methods 0.000 description 2
230000008033 biological extinction Effects 0.000 description 2
239000013060 biological fluid Substances 0.000 description 2
230000005540 biological transmission Effects 0.000 description 2
210000001124 body fluid Anatomy 0.000 description 2
239000010839 body fluid Substances 0.000 description 2
239000000872 buffer Substances 0.000 description 2
230000008859 change Effects 0.000 description 2
229910052729 chemical element Inorganic materials 0.000 description 2
238000007705 chemical test Methods 0.000 description 2
229920001577 copolymer Polymers 0.000 description 2
230000007423 decrease Effects 0.000 description 2
238000001514 detection method Methods 0.000 description 2
239000012153 distilled water Substances 0.000 description 2
238000009826 distribution Methods 0.000 description 2
238000002156 mixing Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
239000007921 spray Substances 0.000 description 2
SLBOQBILGNEPEB-UHFFFAOYSA-N 1-chloroprop-2-enylbenzene Chemical compound C=CC(Cl)C1=CC=CC=C1 SLBOQBILGNEPEB-UHFFFAOYSA-N 0.000 description 1
102000004506 Blood Proteins Human genes 0.000 description 1
108010017384 Blood Proteins Proteins 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
229910010413 TiO 2 Inorganic materials 0.000 description 1
239000002253 acid Substances 0.000 description 1
YBCVMFKXIKNREZ-UHFFFAOYSA-N acoh acetic acid Chemical compound CC(O)=O.CC(O)=O YBCVMFKXIKNREZ-UHFFFAOYSA-N 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
238000013019 agitation Methods 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
239000012482 calibration solution Substances 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
238000009614 chemical analysis method Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
201000001883 cholelithiasis Diseases 0.000 description 1
230000003750 conditioning effect Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
239000013078 crystal Substances 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
230000006866 deterioration Effects 0.000 description 1
239000012954 diazonium Substances 0.000 description 1
150000001989 diazonium salts Chemical class 0.000 description 1
238000006193 diazotization reaction Methods 0.000 description 1
238000009792 diffusion process Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000010410 dusting Methods 0.000 description 1
230000000694 effects Effects 0.000 description 1
230000005670 electromagnetic radiation Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
239000000835 fiber Substances 0.000 description 1
238000011049 filling Methods 0.000 description 1
238000001506 fluorescence spectroscopy Methods 0.000 description 1
208000001130 gallstones Diseases 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
229920001519 homopolymer Polymers 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
239000011229 interlayer Substances 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
238000000691 measurement method Methods 0.000 description 1
239000002609 medium Substances 0.000 description 1
238000002715 modification method Methods 0.000 description 1
239000002736 nonionic surfactant Substances 0.000 description 1
239000002245 particle Substances 0.000 description 1
230000035699 permeability Effects 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
229920003213 poly(N-isopropyl acrylamide) Polymers 0.000 description 1
229920000139 polyethylene terephthalate Polymers 0.000 description 1
239000005020 polyethylene terephthalate Substances 0.000 description 1
239000011148 porous material Substances 0.000 description 1
230000007425 progressive decline Effects 0.000 description 1
230000006920 protein precipitation Effects 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
239000010453 quartz Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
239000012898 sample dilution Substances 0.000 description 1
238000007086 side reaction Methods 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
230000003595 spectral effect Effects 0.000 description 1
238000002798 spectrophotometry method Methods 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
238000009827 uniform distribution Methods 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Landscapes

Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Investigating Or Analysing Biological Materials (AREA)

JP10079879A 1979-08-09 1979-08-09 Fluorescent analysis for bilirubin Granted JPS5524694A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP10079879A JPS5524694A (en)	1979-08-09	1979-08-09	Fluorescent analysis for bilirubin

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP10079879A JPS5524694A (en)	1979-08-09	1979-08-09	Fluorescent analysis for bilirubin

Publications (2)

Publication Number	Publication Date
JPS5524694A JPS5524694A (en)	1980-02-21
JPS6244616B2 true JPS6244616B2 (fr)	1987-09-21

Family

ID=14283416

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP10079879A Granted JPS5524694A (en)	1979-08-09	1979-08-09	Fluorescent analysis for bilirubin

Country Status (1)

Country	Link
JP (1)	JPS5524694A (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5980949A (ja) *	1983-07-01	1984-05-10	Hitachi Ltd	樹脂封止半導体装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5389796A (en) *	1977-01-14	1978-08-07	Eastman Kodak Co	Colorimetric method and detecting element for bilirubin in aqueous solution

1979
- 1979-08-09 JP JP10079879A patent/JPS5524694A/ja active Granted

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5389796A (en) *	1977-01-14	1978-08-07	Eastman Kodak Co	Colorimetric method and detecting element for bilirubin in aqueous solution

Also Published As

Publication number	Publication date
JPS5524694A (en)	1980-02-21

Publication	Publication Date	Title
US4274832A (en)	1981-06-23	Analytical element and method for analysis of multiple analytes
EP0195623B1 (fr)	1992-06-10	Indicateurs fluorescents stabilisés des terres rares et les espèces marqués physiologiquement réactifs
CA1093439A (fr)	1981-01-13	Dosage de la bilirubine
US4828983A (en)	1989-05-09	Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes
EP0860695B1 (fr)	2006-01-04	Procédé pour mesurer un analyte et éprouvette de mesure
EP0008233B1 (fr)	1982-04-14	Méthode fluorométrique pour la détermination de la bilirubine
US5254477A (en)	1993-10-19	Flourescence intramolecular energy transfer conjugate compositions and detection methods
US4069017A (en)	1978-01-17	Colorimetric assay for bilirubin
US6121050A (en)	2000-09-19	Analyte detection systems
JP2651060B2 (ja)	1997-09-10	同時較正不均質イムノアツセイ用装置
US20040110223A1 (en)	2004-06-10	Method for detecting an analyte by fluorescence
US5155024A (en)	1992-10-13	Binder composition and analytical element having stabilized peroxidase in layer containing the composition
US4786605A (en)	1988-11-22	Analytical method and element for protein assay
JP2004517316A (ja)	2004-06-10	被検体の発光光学的測定のための試薬
JPS59228166A (ja)	1984-12-21	試料中の物質の存在を測定する試験具、方法及び試験具の製造方法
US4874692A (en)	1989-10-17	Binder composition and analytical element having stabilized peroxidase in layer containing the composition
JP2003507736A (ja)	2003-02-25	エバネッセンス場法を用いて物質を測定する方法
US4412005A (en)	1983-10-25	Determination of total bilirubin
US4737457A (en)	1988-04-12	Analytical composition, element and method for the determination of hydrogen peroxide
US4230456A (en)	1980-10-28	Element and assay for albumin
JP3446796B2 (ja)	2003-09-16	試験片
DK155349B (da)	1989-03-28	Fremgangsmaade og integralt analytisk legeme til bestemmelse af koncentrationen af en specifik ionisk analyt i en flydende proeve og anvendelse af dette legeme
JPS6244616B2 (fr)	1987-09-21
KR100503562B1 (ko)	2006-02-13	단백질측정용건식분석요소
EP0022673B1 (fr)	1984-12-27	Procédé de détermination sélective de la bilirubine conjuguée ou libre