JPS6240282A - Defoaming device in cultivation vessel of microorganism - Google Patents

Defoaming device in cultivation vessel of microorganism

Info

Publication number
JPS6240282A
JPS6240282A JP18032085A JP18032085A JPS6240282A JP S6240282 A JPS6240282 A JP S6240282A JP 18032085 A JP18032085 A JP 18032085A JP 18032085 A JP18032085 A JP 18032085A JP S6240282 A JPS6240282 A JP S6240282A
Authority
JP
Japan
Prior art keywords
disk
cylinder
orifice
clearance
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP18032085A
Other languages
Japanese (ja)
Other versions
JPH0437713B2 (en
Inventor
Nagahiro Ogasawara
小笠原 長宏
Teru Okawa
大川 輝
Tamitoshi Yamagata
山県 民敏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MARUBISHI BAIONEJI KK
Original Assignee
MARUBISHI BAIONEJI KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MARUBISHI BAIONEJI KK filed Critical MARUBISHI BAIONEJI KK
Priority to JP18032085A priority Critical patent/JPS6240282A/en
Publication of JPS6240282A publication Critical patent/JPS6240282A/en
Publication of JPH0437713B2 publication Critical patent/JPH0437713B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Degasification And Air Bubble Elimination (AREA)

Abstract

PURPOSE:To make it possible to remove foam which flows in the interior of an outer cylinder covering the upper part of a cultivation cylinder with an orifice on the upper wall of a cultivation cylinder, clearance and rotation of a disk and return the defoamed and liquefied fluid into the cultivation cylinder. CONSTITUTION:An orifice 2 is formed on the upper wall of a cultivation cylinder 1 of microorganisms, and a clearance 4 is further held on the top surface of the upper wall to mount a disk 5, which is rotatable by a motor. An outer cylinder 7 for covering the top of the cultivation cylinder 1 is further mounted, and foams which flow into the outer cylinder 7can be removed by the orifice 2, clearance 4 and rotation of the disk 5. Thus, foams are removed in the clearance 4 while interfered by the rotation of the disk 5 to collide with the inner wall of the outer cylinder 7, and the foams are further removed and liquefied. The fluid is then fed back into the cultivation cylinder 1.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 この発明は微生物の培養器に於ける消泡装置に関するも
のであり、特に微生物培養筒内に発生する泡を消泡して
部外と泡との接触を遮断せしめんとする消泡iaに関す
るものである。
[Detailed Description of the Invention] [Industrial Application Field] This invention relates to a defoaming device for a microorganism incubator, and in particular, it defoams the foam generated inside the microbial culture cylinder and separates the foam from the outside. This relates to an antifoaming ia that attempts to cut off contact with the diaphragm.

〔従来の技術〕[Conventional technology]

従来、培養筒内に於て培養が進むに従って、該筒内に泡
が発生するが、この泡の上面にディスクを設け、前記泡
の下面より培養液シ取出し、前記ディスクの上面に吹付
け、更にこの吹付けらh6た培養液が培養筒の内壁に水
平に吹付けら刺前記ディスクと筒壁間に一種の液膜を形
成せしめ−この液膜の下方に発生する泡はこの液膜によ
って上方に上昇せんとするのを遮断して、前記発生した
泡と外気との接触?防止するように形成されていた。
Conventionally, as the culture progresses in a culture cylinder, bubbles are generated in the cylinder, and a disk is provided above the bubbles, and the culture solution is taken out from the bottom surface of the bubbles and sprayed onto the top surface of the disk. Furthermore, this sprayed culture solution is sprayed horizontally onto the inner wall of the culture cylinder, forming a kind of liquid film between the disk and the cylinder wall - the bubbles generated below this liquid film are swept upward by this liquid film. Is it possible to block the bubbles from rising and contact the generated bubbles with the outside air? It was designed to prevent.

〔発明が解決1−ようとする問題点〕 従来の装置は前述したような構成?有し、且つ、発生し
た泡と外気との接触を断つように試みられてはいるが、
現実には前記ディスクにより吹付けらねた泡は培養筒の
内壁に@撃される時、返って泡を増殖する結果となり、
更に又、発泡が大きくなるに従い、ディスクの回転を大
ぎくせざるを得なくなり、従って該ディスクにかかる負
荷も又。
[Problem that the invention attempts to solve 1] Does the conventional device have the above-mentioned configuration? Although attempts have been made to cut off the contact between the generated bubbles and the outside air,
In reality, when the bubbles sprayed by the disk are struck against the inner wall of the culture tube, they return and multiply as bubbles.
Furthermore, as the foaming increases, the rotation of the disk must be increased, and therefore the load placed on the disk also increases.

大きくならざるを得なくなり、更に又、培養筒の中に送
入されるエアー量が大きい時は、発生した泡自体が前記
ディスクによって形成された液膜を破り、上方に逃げる
現象が往々にして発生していた。又、培養筒の内径が大
になわば、当然に前記ディスクも大径のものケ使用せざ
るを得なくなり回転数も大とせざるな得ないのであり、
その為。
Moreover, when the amount of air introduced into the culture tube is large, the bubbles themselves often break through the liquid film formed by the disk and escape upwards. It was occurring. Furthermore, if the inner diameter of the culture cylinder becomes larger, it is natural that the disk must have a larger diameter and the rotational speed must also be increased.
For that reason.

動力が大となり消費電力が増すのである。更に又、培養
筒の中で発生する泡は前述せろ如く、ディスクの回転に
伴って生じる液膜によって上方への流出が遮断せら幻る
ことになるのであるが、当該泡は肖然@紀液膜に接触す
る事により遮断させられる。この時、該液膜に1触する
泡は湿分の多い泡に形成されることになり、従って、湿
分の多い当核泡を消泡することは極めて困難性を伴うの
である。
As the power increases, power consumption increases. Furthermore, as mentioned above, the bubbles generated in the culture tube become invisible because the upward flow is blocked by the liquid film that is generated as the disk rotates, but the bubbles are It is shut off by contacting the membrane. At this time, a bubble that touches the liquid film will form a foam with a high moisture content, and therefore, it is extremely difficult to defoam the core foam with a high moisture content.

〔問題点を解決する為の手段〕[Means for solving problems]

この発明は前記従来の装置の欠陥&Cviみこれを解消
せんとして提案せら幻だものであり、微生物の培養筒の
上壁にオリフィスを形成し、更に前記上壁の上面にクリ
アランス?保有してディスクを取付け、このディスクは
モータによって回転出来るようにし、更に前記培養筒の
上部ケ被蔽する外筒な取付け、この外筒内に流入した泡
を前記オリフィスとクリアランス並びにディスクの回転
によって消泡出来るよ5にl、た事を特徴とする微生物
培IP器に於ける消泡装置を提供せんとするものである
This invention is a phantom that was proposed to solve the defects and Cvi of the conventional device, and an orifice is formed on the upper wall of the microorganism culture cylinder, and a clearance is provided on the upper surface of the upper wall. The disk is rotated by a motor, and an outer cylinder is attached to cover the top of the culture cylinder, and the bubbles flowing into the outer cylinder are removed by the orifice and the clearance and the rotation of the disk. It is an object of the present invention to provide a defoaming device for a microbial culture IP vessel, which is capable of defoaming.

〔作用〕[Effect]

この発明によ幻ば、培養筒中に発生1.た泡は筒内の圧
力と部外の圧力差忙よりオリフィスを介1゜て上方に上
昇する。この上昇した泡は、ディスクとの間のクリアラ
ンスを通過しながらディスクの円周方向に流出しようと
する。この時、前記ディスクを回転する事により前記泡
はクリアランス間に於てディスクの回転により干渉され
乍ら消泡され、外筒の内壁に衝突して更に消泡さ幻て液
化し、この液は前記培養筒の中にフィードバックせらね
る事になるのである。
According to this invention, occurrence of 1. The bubbles rise upward through the orifice due to the difference between the pressure inside the cylinder and the pressure outside. This rising bubble attempts to flow out in the circumferential direction of the disk while passing through the clearance between the bubble and the disk. At this time, by rotating the disk, the bubbles are dissipated while being interfered with by the rotation of the disk between the clearances, collide with the inner wall of the outer cylinder, further dissipated and liquefied, and this liquid is This results in feedback flowing into the culture cylinder.

〔実施例〕〔Example〕

以下、この発明の一実施例?別紙添付図面によって詳述
する。(1)は微生物の培養筒であり、この実施例は金
桟製によって形成l−ているが、これに限定せられるぺ
ぎものではない。(2)はオリフィスである。この実施
例図では肖誼オリアイス(2)か形成するに当っては、
前記培養筒(1)の上Q(la)の中心部に開穿せられ
た孔部(3)の内壁に螺設部(3a)t/設け、この螺
設部に前記オリフィス(2)な中心部に形成したオリフ
ィス片(2a)+4着して形成しである。このオリフィ
スの径は%6ψ、10ψ、20φを可とするがこれに限
定させらhるものではない。
Is the following an example of this invention? This will be explained in detail with reference to the attached drawings. Reference numeral (1) is a microorganism culture cylinder, and in this embodiment it is made of metal rails, but the cylinder is not limited to this. (2) is an orifice. In this example figure, when forming the portrait oriais (2),
A threaded portion (3a) is provided on the inner wall of the hole (3) drilled in the center of the upper Q(la) of the culture tube (1), and the orifice (2) is inserted into this threaded portion. The orifice piece (2a) is formed in the center and 4 pieces are formed. The diameter of this orifice may be 6φ, 10φ, or 20φ, but is not limited to these.

更に、1itJ記上磯(la)に形成せら幻るオリフィ
スは極めて小径のもの?多数形成してもよい。(4)は
上記培養筒(1)の土壁(1a)の上面Vckll付け
らねたディスクC5)との間に設けら幻だクリアランス
である。
Furthermore, is the orifice that appears to be formed in Kamiso (la) in 1itJ extremely small in diameter? A large number may be formed. (4) is the clearance provided between the upper surface of the soil wall (1a) of the culture tube (1) and the disk C5) attached to the top surface of the soil wall (1a).

更に、ディスク(5)は前記オリフィス片にこのディス
ク(5)の軸(6)を配設してモーター並びに減速機を
介して形成されている。更に(7)は前記培養筒(1)
の上方部を被蔽するように密封して取付けられた外筒で
あり、而も、その床板(7a)は前記培養筒(1)の外
壁に浴接して取付けであるが、その取付方法は之に限定
されるべきではない。更に土壁(1a)は軸シールな介
して前記ディスク(5)の軸(6)を挿通せられている
。更にこの土壁(1a)の適宜個所にエアー抜き用のパ
イプ(7C)が取付けら幻ている。又、この外筒(7)
の上方部側壁(7d)はシール等により透明体を嵌着t
、て取付けら刺ているが、こわて限定さ幻るべきもので
はない。更に、この外筒(7)の内槽のほぼ中間部に於
て、l−かも前記ディ       Iスフ(5)の円
周方向の位業から縦方向に縦設せられた邪麿板(8)+
8)・・・が処々に形成せられている。更にこの外筒の
前、記底板(7a)は斜設せられており、その下端部に
液のフィードバック用のパイプ(9)(9)・・・が取
付けら幻ていて、このパイプの他端部は前記培養筒(1
)に挿通せられている。尚、図中符号α0はシード挿入
口であり、allは冷却並びに加熱用の二重筒、(ll
a)は水又は蒸気の出入口、  (11b%’l水又は
蒸気の出入口である。
Furthermore, the disk (5) is formed by arranging the shaft (6) of the disk (5) in the orifice piece through a motor and a speed reducer. Furthermore, (7) is the culture tube (1)
It is an outer cylinder that is sealed and installed so as to cover the upper part, and its floor plate (7a) is installed in contact with the outer wall of the culture cylinder (1), but the method of installation is as follows. It should not be limited to this. Furthermore, the shaft (6) of the disk (5) is inserted through the earthen wall (1a) through a shaft seal. Furthermore, air vent pipes (7C) are installed at appropriate locations on this earthen wall (1a). Also, this outer cylinder (7)
A transparent body is fitted onto the upper side wall (7d) with a seal etc.
Although it is difficult to install, it is not something to be afraid of. Further, at approximately the middle of the inner tank of the outer cylinder (7), a vertical plate (8) is installed vertically from the circumferential position of the disk (5). )+
8)... are formed here and there. Furthermore, in front of this outer cylinder, the bottom plate (7a) is installed diagonally, and pipes (9) (9) for liquid feedback are attached to the lower end of the bottom plate (7a). The end part is attached to the culture cylinder (1
) is inserted. In addition, the symbol α0 in the figure is a seed insertion port, all is a double cylinder for cooling and heating, (ll is
a) is an inlet/outlet for water or steam, (11b%'l an inlet/outlet for water or steam).

その他、この培養筒(1)にはエアーの入口、培地の入
口等?有することは当然である。更に、培養筒(!)の
下底より回転軸a3を挿入して回転体(13を取付け、
前記培養筒内の液を攪拌できるように形成されている。
In addition, is there an air inlet, medium inlet, etc. in this culture cylinder (1)? It is natural to have one. Furthermore, insert the rotating shaft a3 from the bottom of the culture cylinder (!) and attach the rotating body (13).
It is formed so that the liquid in the culture cylinder can be stirred.

この発明の一実施例は叙述せる如ぎ構成にかかるから、
先づ、培地投入口より培地を投入し加熱l−て滅菌し、
更にこれを適正温度まで冷却し、上方のシート0の挿入
口よりシードを投入し攪拌等により菌が培養せられる。
One embodiment of this invention has the configuration as described,
First, a medium is poured into the medium inlet and sterilized by heating.
This is further cooled to an appropriate temperature, seeds are introduced from the insertion opening of the upper sheet 0, and bacteria are cultured by stirring or the like.

この培養筒(1)の内部に於ては培養が進むに従い培地
の上方部に泡が発生する。この泡は、培養筒(1)内の
圧力と前記外筒(7)内との圧力差によって、当然前記
発生した泡は前記培養筒(1)の上壁(1a)の中心部
に形成せられたオリフィス(2)より前記外筒(7)内
に進入しようとするのである。この時、オリフィス(2
)より進′大した泡はディスク(5)とのクリアランス
(4)を介して前記ディスクの円鳩方回に通過しようと
するが、前記ディスク(5)は適宜の回転をしているの
で、当接クリアランスC4)を通過する泡はディスク(
5)の回転により。
Inside this culture cylinder (1), as the culture progresses, bubbles are generated in the upper part of the medium. These bubbles are caused by the pressure difference between the pressure inside the culture cylinder (1) and the inside of the outer cylinder (7), and naturally the generated bubbles are formed in the center of the upper wall (1a) of the culture cylinder (1). It attempts to enter the outer cylinder (7) through the orifice (2). At this time, the orifice (2
) The more advanced bubbles try to pass through the clearance (4) between the disk (5) and the disk (5), but since the disk (5) is rotating appropriately, Bubbles passing through the contact clearance C4) will pass through the disc (
5) by rotation.

回転方向に放散せられる。この状態で大部分の泡は消泡
せら幻る場合が多い。更に前記ディスク(5)の回転数
を上げれば消泡効果は更に助成せら幻るのである。
Dissipated in the direction of rotation. In this state, most of the foam often disappears. Furthermore, if the rotational speed of the disk (5) is increased, the defoaming effect will be further enhanced.

更に又、前記ディスク(5)により放敗せられた泡状物
は外筒(7)の内壁に縦設せられた邪魔板(8)(8)
・・・に当接15.この干渉によって令状の泡は更忙消
泡され全体とl、て液化せら幻、るよ5になる。斯くし
て、液化せられた状態で前記邪魔板により垂直に下方に
流下するようになるのである。この流下した液は底い個
所に流下さh、フィードバック用のパイプ(9H9)・
・・により前記培養筒(11にフィードバックさせられ
る。この時1重要な事は前記外筒(7)の土壁(1a)
に形成せられているエアー抜用パイプ(7c)内には一
切前記泡は流入しないのであり、従来のように当該泡を
介して雑菌が侵入したり、池内の有用菌が部外に逃散す
るような事は全(防止できる。
Furthermore, the foam discharged by the disk (5) is removed by baffle plates (8) (8) vertically installed on the inner wall of the outer cylinder (7).
Contact with...15. As a result of this interference, the foam of the warrant is further defoamed and becomes liquefied as a whole. In this way, the liquefied liquid flows vertically downward by the baffle plate. This liquid flows down to the bottom part, and the feedback pipe (9H9)
... is fed back to the culture tube (11).At this time, the first important thing is that the soil wall (1a) of the outer tube (7)
The bubbles do not flow into the air vent pipe (7c) formed in the air vent pipe (7c), and as in the conventional case, bacteria may enter through the bubbles, and useful bacteria in the pond may escape to the outside. All of these things can be prevented.

〔発明の効果〕〔Effect of the invention〕

この発明は、オリフィスとクリアランスと、ディスク並
びにこのディスクの回転によって培養筒内に発生した泡
を物理的に消泡することができ。
This invention can physically eliminate bubbles generated in the culture cylinder by the orifice, the clearance, the disk, and the rotation of the disk.

従来からの懸案事項となっている諸種の欠陥を悉く一掃
できるようになる等、正に画期的発明を構成するもので
ある。
This truly constitutes an epoch-making invention, as it makes it possible to eliminate all the various defects that have been a concern in the past.

【図面の簡単な説明】 1に本発明の一実施例を示し、91図は正面図、m2図
は側面図、纂3図は縦断正面図である0 符号説明 (1)・・・・・・培養筒 (2ト・・・・・オリフィス (a・・・・・・クリアランス (5)・・・・・・ディスク (7)・・・・・・外筒 (8)・・・・・・邪魔板
[Brief Description of the Drawings] 1 shows an embodiment of the present invention, in which Figure 91 is a front view, Figure m2 is a side view, and Figure 3 is a longitudinal front view.0 Explanation of symbols (1)...・Culture tube (2 pieces)... Orifice (a... Clearance (5)... Disk (7)... Outer tube (8)...・Baffle board

Claims (1)

【特許請求の範囲】[Claims] 微生物の培養筒の上壁にオリフィスを形成し、更に前記
上壁の上面にクリアランスを保有してディスクを取付け
、このディスクはモータによって回転出来るようにし、
更に前記培養筒の上部を被蔽する外筒を取付け、この外
筒内に流入した泡を前記オリフィスとクリアランス並び
にディスクの回転によって消泡出来るようにした事を特
徴とする微生物培養器に於る消泡装置。
An orifice is formed on the upper wall of the microorganism culture cylinder, and a disk is mounted on the upper surface of the upper wall with a clearance, and the disk is rotatable by a motor.
The microorganism incubator is further characterized in that an outer cylinder is attached to cover the upper part of the culture cylinder, and bubbles flowing into the outer cylinder can be defoamed by the clearance between the orifice and the rotation of the disk. Defoaming device.
JP18032085A 1985-08-16 1985-08-16 Defoaming device in cultivation vessel of microorganism Granted JPS6240282A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP18032085A JPS6240282A (en) 1985-08-16 1985-08-16 Defoaming device in cultivation vessel of microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18032085A JPS6240282A (en) 1985-08-16 1985-08-16 Defoaming device in cultivation vessel of microorganism

Publications (2)

Publication Number Publication Date
JPS6240282A true JPS6240282A (en) 1987-02-21
JPH0437713B2 JPH0437713B2 (en) 1992-06-22

Family

ID=16081147

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18032085A Granted JPS6240282A (en) 1985-08-16 1985-08-16 Defoaming device in cultivation vessel of microorganism

Country Status (1)

Country Link
JP (1) JPS6240282A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10149132A1 (en) * 2001-10-05 2003-08-07 Sartorius Gmbh Method and device for defoaming a bioreactor
CN105264363A (en) * 2013-03-29 2016-01-20 株式会社爱茉莉太平洋 Device and method for detecting bubbles of vacuum container for manufacturing cosmetic contents, and recording medium for storing program code for executing method thereof by computer

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10149132A1 (en) * 2001-10-05 2003-08-07 Sartorius Gmbh Method and device for defoaming a bioreactor
DE10149132B4 (en) * 2001-10-05 2006-05-04 Sartorius Ag Device for defoaming a bioreactor
CN105264363A (en) * 2013-03-29 2016-01-20 株式会社爱茉莉太平洋 Device and method for detecting bubbles of vacuum container for manufacturing cosmetic contents, and recording medium for storing program code for executing method thereof by computer

Also Published As

Publication number Publication date
JPH0437713B2 (en) 1992-06-22

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