JPH0437713B2 - - Google Patents
Info
- Publication number
- JPH0437713B2 JPH0437713B2 JP18032085A JP18032085A JPH0437713B2 JP H0437713 B2 JPH0437713 B2 JP H0437713B2 JP 18032085 A JP18032085 A JP 18032085A JP 18032085 A JP18032085 A JP 18032085A JP H0437713 B2 JPH0437713 B2 JP H0437713B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- disk
- cylinder
- bubbles
- orifice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000006260 foam Substances 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 6
- 239000007788 liquid Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- 241000758760 Porteria Species 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Degasification And Air Bubble Elimination (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明は微生物の培養器に於ける消泡装置に
関するものであり、特に微生物培養筒内に発生す
る泡を消泡して筒外と泡との接触を遮断せしめん
とする消泡装置に関するものである。[Detailed Description of the Invention] [Industrial Application Field] This invention relates to a defoaming device for a microorganism incubator, and in particular, it defoams the foam generated inside the microbial culture cylinder and removes the foam from the outside of the cylinder. This invention relates to a defoaming device that attempts to cut off contact with the air.
従来、培養筒内に於て培養が進むに従つて、該
筒内に泡が発生するが、この泡の上面にデイスク
を設け、前記泡の下面より培養液を取出し、前記
デイスクの上面に吹付け、更にこの吹付けられた
培養液が培養筒の内壁に水平に吹付けられ前記デ
イスクと筒壁間に一種の液膜を形成せしめ、この
液膜の下方に発生する泡はこの液膜によつて上方
に上昇せんとするのを遮断して、前記発生した泡
と外気との接触を防止するように形成されてい
た。
Conventionally, as the culture progresses in a culture cylinder, bubbles are generated in the cylinder, and a disk is provided above the bubbles, and the culture solution is taken out from the bottom of the bubbles and blown onto the top surface of the disk. Furthermore, this sprayed culture solution is sprayed horizontally onto the inner wall of the culture cylinder, forming a kind of liquid film between the disk and the cylinder wall, and the bubbles generated below this liquid film are absorbed by this liquid film. The bubbles are formed so as to block the bubbles from rising upward, thereby preventing the generated bubbles from coming into contact with the outside air.
従来の装置は前述したような構成を有し、且
つ、発生した泡と外気との接触を断つように試み
られてはいるが、現実には前記デイスクにより吹
付けられた泡は培養筒の内壁に衝撃される時、返
つて泡を増殖する結果となり、更に又、発泡が大
きくなるに従い、デイスクの回転を大きくせざる
を得なくなり、従つて該デイスクにかかる負荷も
又、大きくならざるを得なくなり、更に又、培養
筒の中に送入されるエアー量が大きい時は、発生
した泡自体が前記デイスクによつて形成された液
膜を破り、上方に逃げる現象が往々にして発生し
ていた。又、培養筒の内径が大になれば、当然に
前記デイスクも大径のものを使用せざるを得なく
なり回転数も大とせざるを得ないのであり、その
為、動力が大となり消費電力が増すのである。更
に又、培養筒の中で発生する泡は前述せる如く、
デイスクの回転に伴つて生じる液膜によつて上方
への流出が遮断せられることになるのであるが、
当該泡は当然前記液膜に接触する事により遮断さ
せられる。この時、該液膜に接触する泡は湿分の
多い泡に形成されることになり、従つて、湿分の
多い当該泡を消泡することは極めて困難性を伴う
のである。
Conventional devices have the above-mentioned configuration, and although attempts have been made to cut off contact between the generated bubbles and the outside air, in reality, the bubbles blown by the disk are attached to the inner wall of the culture tube. When the disk is impacted by air, it results in the proliferation of bubbles, and furthermore, as the bubbles increase, the rotation of the disk has to be increased, and the load on the disk also has to increase. Furthermore, when the amount of air fed into the culture tube is large, the bubbles themselves often break through the liquid film formed by the disk and escape upwards. Ta. Furthermore, if the inner diameter of the culture tube becomes larger, the disk must also have a larger diameter and the rotational speed must also be increased, which results in increased power and power consumption. It increases. Furthermore, as mentioned above, the bubbles generated in the culture tube are
The upward flow is blocked by the liquid film that forms as the disk rotates.
Naturally, the bubbles are blocked by coming into contact with the liquid film. At this time, the bubbles that come into contact with the liquid film are formed into bubbles with a high moisture content, and therefore, it is extremely difficult to defoam the bubbles with a high moisture content.
この発明は前記従来の装置の欠陥に鑑みこれを
解消せんとして提案せられたものであり、微生物
の培養筒の上壁にオリフイスを形成し、更に、該
培養筒の前記上壁の直近に該上壁と平行なデスク
を設置し、且つ、該培養筒上壁とデスクとの間の
クリアランスは、該培養筒の上部を被蔽する外筒
内の空間部と連通し、更に、前記デスクはモータ
にて回転自在に形成されており、前記培養筒内に
生じる泡が前記オリフイスを介してクリアランス
に流入し、更に、前記外筒内に進入するとき、該
デスクの回転にて該泡を該デスクの回転方向に放
散せしめて消泡できるように構成されたことを特
徴とする微生物の培養器に於ける消泡装置を提供
せんとするものである。
This invention was proposed in view of the above-mentioned deficiencies of the conventional apparatus, and was proposed in order to solve the problems. A desk is installed parallel to the top wall, and a clearance between the top wall of the culture tube and the desk communicates with a space inside the outer tube that covers the top of the culture tube, and furthermore, the desk is The disc is rotatable by a motor, and when the bubbles generated in the culture cylinder flow into the clearance via the orifice and further enter the outer cylinder, the rotation of the desk causes the bubbles to be removed. It is an object of the present invention to provide a defoaming device for a microorganism incubator, characterized in that the defoaming device is configured to dissipate foam by dispersing it in the direction of rotation of a desk.
この発明によれば、培養筒中に発生した泡は筒
内の圧力と筒外の圧力差によりオリフイスを介し
て上方に上昇する。この上昇した泡は、デイスク
との間のクリアランスを通過しながらデイスクの
円周方向に流出しようとする。この時、前記デイ
スクを回転する事により前記泡はクリアランス間
に於てデイスクの回転により干渉され乍ら消泡さ
れ、外筒の内壁に衝突して更に消泡されて液化
し、この液は前記培養筒の中にフイードバツクせ
られる事になるのである。
According to this invention, bubbles generated in the culture cylinder rise upward through the orifice due to the difference in pressure between the inside of the cylinder and the pressure outside the cylinder. This rising bubble attempts to flow out in the circumferential direction of the disk while passing through the clearance between the bubble and the disk. At this time, by rotating the disc, the foam is defoamed while being interfered with by the rotation of the disc between the clearances, collides with the inner wall of the outer cylinder, and is further defoamed and liquefied, and this liquid is Feedback will be fed into the culture tube.
以下、この発明の一実施例を別紙添付図面によ
つて詳述する。1は微生物の培養筒であり、この
実施例は金属製によつて形成しているが、これに
限定せられるべきものではない。2はオリフイス
である。この実施例図では当該オリフイス2を形
成するに当つては、前記培養筒1の上壁1aの中
心部に開穿せられた孔部3の内壁に螺設部3aを
設け、この螺設部に前記オリフイス2を中心部に
形成したオリフイス片2aを螺着して形成してあ
る。このオリフイスの径は、6φ、10φ、20φを可
とするがこれに限定させられるものではない。更
に、前記上壁1aに形成せられるオリフイスは極
めて小径のものを多数形成してもよい。4は上記
培養筒1の上壁1aの上面に取付けられたデイス
ク5との間に設けられたクリアラスである。更
に、デイスク5は前記オリフイス2の中心部にこ
のデイスク5の軸6を配設してモーター並びに減
速機を介して形成されている。更に7は前記培養
筒1の上方部を被蔽するように密封して取付けら
れた外筒であり、而も、その底板7aは前記培養
筒1の外壁に溶接して取付けてあるが、その取付
方法は之に限定されるべきではない。更に上壁1
aは軸シールを介して前記デイスク5の軸6を挿
通さられている。更にこの上壁1aの適宜個所に
エア−抜き用のパイプ7cが取付けられている。
又、この外筒7の上方部側壁7dはシール等によ
り透明体を嵌着して取付けられているが、これに
限定されるべきものではない。更に、この外筒7
の内壁のほぼ中間部に於て、しかも前記デイスク
5の円周方向の位置から縦方向に縦設せられた邪
摩板8,8……が処々に形成せられている。更に
この外筒の前記底板7aは斜設せられており、そ
の下端部に液のフイードバツクの用のパイプ9,
9……が取付けられていて、このパイプの他端部
は前記培養筒1に挿通せられている。尚、図中符
号10はシード挿入口であり、11は冷却並びに
加熱用の二重筒、11aは水又は蒸気の出入口、
11bは水又は蒸気の出入口である。
Hereinafter, one embodiment of the present invention will be described in detail with reference to the accompanying drawings. Reference numeral 1 denotes a microorganism culture cylinder, which is made of metal in this embodiment, but is not limited to this. 2 is an orifice. In this embodiment figure, when forming the orifice 2, a threaded portion 3a is provided on the inner wall of the hole 3 bored in the center of the upper wall 1a of the culture cylinder 1, and this threaded portion An orifice piece 2a having the orifice 2 formed in the center thereof is screwed onto the orifice piece 2a. The diameter of this orifice may be 6φ, 10φ, or 20φ, but is not limited to these. Furthermore, a large number of orifices having extremely small diameters may be formed in the upper wall 1a. Reference numeral 4 denotes a clear glass provided between the culture tube 1 and the disk 5 attached to the upper surface of the upper wall 1a. Further, the disk 5 is formed by disposing a shaft 6 of the disk 5 in the center of the orifice 2 via a motor and a speed reducer. Furthermore, 7 is an outer cylinder that is sealed and attached to cover the upper part of the culture tube 1, and its bottom plate 7a is attached by welding to the outer wall of the culture tube 1. The mounting method should not be limited to this. Furthermore, upper wall 1
The shaft 6 of the disk 5 is inserted through the shaft 6 of the disk 5 through a shaft seal. Furthermore, air venting pipes 7c are attached to appropriate locations on the upper wall 1a.
Further, the upper side wall 7d of the outer cylinder 7 is attached with a transparent body fitted with a seal or the like, but the present invention is not limited to this. Furthermore, this outer cylinder 7
At approximately the middle of the inner wall of the disk 5, there are formed in many places vertically disposed vertically extending baffle plates 8, 8, . . . from the circumferential position of the disk 5. Furthermore, the bottom plate 7a of this outer cylinder is installed obliquely, and a pipe 9 for liquid feedback is provided at its lower end.
9... is attached, and the other end of this pipe is inserted into the culture cylinder 1. In addition, the reference numeral 10 in the figure is a seed insertion port, 11 is a double cylinder for cooling and heating, 11a is an inlet/outlet for water or steam,
11b is an inlet/outlet for water or steam.
その他、この培養筒1にはエアーの入口、培地
の入口等を有することは当然である。更に、培養
筒1の下底より回転軸12を挿入して回転体13
を取付け、前記培養筒内の液を撹拌できるように
形成されている。 In addition, it goes without saying that the culture tube 1 has an air inlet, a culture medium inlet, and the like. Furthermore, the rotating shaft 12 is inserted from the bottom of the culture cylinder 1, and the rotating body 13 is
It is formed so that it can be attached to the tube and stir the liquid in the culture tube.
この発明の一実施例は叙述せる如き構成にかか
るから、先づ、培地投入口より培地を投入して加
熱して滅菌し、更にこれを適正温度まで冷却し、
上方のシードの挿入口よりシードを投入し撹拌等
により菌が培養せられる。この培養筒1の内部に
於ては培養が進むに従い培地の上方部に泡が発生
する。この泡は、培養筒1内の圧力と前記外筒7
内との圧力差によつて、当然前記発生した泡は前
記培養筒1の上壁1aの中心部に形成せられたオ
リフイス2より前記外筒7内に進入しようとする
のである。この時、オリフイス2より進入した泡
はデイスク5とのクリアランス4を介して前記デ
イスのク円周方向に通過しようとするが、前記デ
イスク5は適宜の回転をしているので、当該クリ
アランス4を通過する泡はデイスク5の回転によ
り、回転方向に放散せられる。この状態で大部分
の泡は消泡せられる場合が多い。更に前記デイス
ク5の回転数を上げれば消泡効果は更に助成せら
れるのである。 Since one embodiment of the present invention has the configuration described above, first, a culture medium is introduced from the culture medium input port, heated and sterilized, and further cooled to an appropriate temperature.
Seeds are introduced from the upper seed insertion port and bacteria are cultured by stirring, etc. Inside the culture cylinder 1, as the culture progresses, bubbles are generated in the upper part of the medium. This bubble is caused by the pressure inside the culture tube 1 and the outer tube 7.
Due to the pressure difference between the inside and the inside, the generated bubbles naturally tend to enter the outer cylinder 7 through the orifice 2 formed in the center of the upper wall 1a of the culture cylinder 1. At this time, the bubbles entering from the orifice 2 try to pass in the circumferential direction of the disk via the clearance 4 between the disk 5 and the disk 5, but since the disk 5 is rotating appropriately, the bubbles enter through the clearance 4. The passing bubbles are dispersed in the rotational direction by the rotation of the disk 5. In this state, most of the foam is often defoamed. Furthermore, by increasing the rotational speed of the disk 5, the defoaming effect can be further enhanced.
更に又、前記デイスク5により放散せられた泡
状物は外筒7の内壁に縦設せられた邪摩板8,8
……に当接し、この干渉によつて余状の泡は更に
消泡され全体として液化せられるようになる。斯
くして、液化せられた状態で前記邪摩板により垂
直に下方に流下するようになるのである。この流
下した液は底い個所に流下され、フイードバツク
用のパイプ9,9……により前記培養筒1にフイ
ードバツクさせられる。この時、重要な事は前記
外筒7の上壁1aに形成せられているエアー抜用
パイプ7c内には一切前記泡は流入しないのであ
り、従来のように当該泡を介して雑菌が侵入した
り、泡内の有用菌が筒外に逃散するような事は全
く防止できる。 Furthermore, the foam dissipated by the disk 5 is dissipated by the interference plates 8, 8 vertically installed on the inner wall of the outer cylinder 7.
..., and due to this interference, the remaining foam is further defoamed and the entire foam is liquefied. In this way, the liquefied water flows vertically downward due to the jamming plate. This liquid flows down to the bottom and is fed back to the culture tube 1 through the feedback pipes 9, 9, . . . . At this time, the important thing is that the bubbles do not flow into the air venting pipe 7c formed on the upper wall 1a of the outer cylinder 7, and bacteria enter through the bubbles as in the conventional case. It is completely possible to prevent the useful bacteria inside the bubbles from escaping outside the cylinder.
この発明は、オリフイスとクリアランスと、デ
イスク並びにこのデイスクの回転によつて培養筒
内に発生した泡を物理的に消泡することができ、
従来からの懸案事項となつている諸種の欠陥を悉
く一掃できるようになる等、正に画期的発明を構
成するものである。
This invention is capable of physically defoaming bubbles generated in the culture cylinder by the orifice, the clearance, the disk, and the rotation of the disk.
This truly constitutes an epoch-making invention, as it has become possible to eliminate all the various defects that have been a concern in the past.
図は本発明の一実施例を示し、第1図は正面
図、第2図は側面図、第3図は縦断正面図であ
る。
符号説明、1……培養筒、2……オリフイス、
4……クリアランス、5……デイスク、7……外
筒、8……邪摩板。
The drawings show one embodiment of the present invention, in which FIG. 1 is a front view, FIG. 2 is a side view, and FIG. 3 is a longitudinal sectional front view. Code explanation, 1...Culture cylinder, 2...Orifice,
4...Clearance, 5...Disc, 7...Outer cylinder, 8...Japanese plate.
Claims (1)
し、更に、該培養筒の前記上壁の直近に該上壁と
平行なデスクを設置し、且つ、該培養筒上壁とデ
スクとの間のクリアランスは、該培養筒の上部を
被蔽する外筒内の空間部と連通し、更に、前記デ
スクはモータにて回転自在に形成されており、前
記培養筒内に生じる泡が前記オリフイスを介して
クリアランスに流入し、更に、前記外筒内に進入
するとき、該デスクの回転にて該泡を該デスクの
回転方向に放散せしめて消泡できるように構成さ
れたことを特徴とする微生物の培養器に於ける消
泡装置。1. An orifice is formed on the upper wall of the microorganism culture cylinder, and a desk parallel to the upper wall is installed in the immediate vicinity of the upper wall of the culture cylinder, and a space between the upper wall of the culture cylinder and the desk is provided. The clearance communicates with a space inside the outer cylinder that covers the upper part of the culture cylinder, and the desk is rotatable by a motor, so that bubbles generated in the culture cylinder are passed through the orifice. The microorganism is configured such that when the microorganism enters the clearance and further enters the outer cylinder, the foam is dissipated in the direction of rotation of the disk by rotation of the disk, thereby defoaming. Defoaming device in culture vessels.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18032085A JPS6240282A (en) | 1985-08-16 | 1985-08-16 | Defoaming device in cultivation vessel of microorganism |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18032085A JPS6240282A (en) | 1985-08-16 | 1985-08-16 | Defoaming device in cultivation vessel of microorganism |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6240282A JPS6240282A (en) | 1987-02-21 |
JPH0437713B2 true JPH0437713B2 (en) | 1992-06-22 |
Family
ID=16081147
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP18032085A Granted JPS6240282A (en) | 1985-08-16 | 1985-08-16 | Defoaming device in cultivation vessel of microorganism |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6240282A (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10149132B4 (en) * | 2001-10-05 | 2006-05-04 | Sartorius Ag | Device for defoaming a bioreactor |
KR101441884B1 (en) * | 2013-03-29 | 2014-09-25 | (주)아모레퍼시픽 | A Bubble Detecting Unit of Vacuum Container for Manufacturing Cosmetic and Method Thereof and Program Recording Unit Thereof |
-
1985
- 1985-08-16 JP JP18032085A patent/JPS6240282A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS6240282A (en) | 1987-02-21 |
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