JPS62246591A - Production of extraction of active ingredient in gymnema-sylvestre r-br - Google Patents
Production of extraction of active ingredient in gymnema-sylvestre r-brInfo
- Publication number
- JPS62246591A JPS62246591A JP61087128A JP8712886A JPS62246591A JP S62246591 A JPS62246591 A JP S62246591A JP 61087128 A JP61087128 A JP 61087128A JP 8712886 A JP8712886 A JP 8712886A JP S62246591 A JPS62246591 A JP S62246591A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- gymnema
- active ingredient
- water
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004480 active ingredient Substances 0.000 title claims abstract description 17
- 241000208253 Gymnema sylvestre Species 0.000 title claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 238000000605 extraction Methods 0.000 title abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000000284 extract Substances 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000126 substance Substances 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract 3
- 241000208251 Gymnema Species 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 14
- 238000005187 foaming Methods 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000000469 ethanolic extract Substances 0.000 description 3
- 229930183009 gymnemic acid Natural products 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- VKJLHZZPVLQJKG-ABHKXHSUSA-N (3r,4r,4ar,5s,6ar,6as,6br,8ar,9r,10s,12ar,14bs)-4a,9-bis(hydroxymethyl)-2,2,6a,6b,9,12a-hexamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-3,4,5,10-tetrol Chemical compound C([C@@]12C)C[C@H](O)[C@@](C)(CO)[C@@H]1CC[C@]1(C)[C@@H]2CC=C2[C@@H]3CC(C)(C)[C@@H](O)[C@H](O)[C@]3(CO)[C@@H](O)C[C@]21C VKJLHZZPVLQJKG-ABHKXHSUSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000006286 aqueous extract Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 241000208327 Apocynaceae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- VKJLHZZPVLQJKG-UHFFFAOYSA-N Theasapogenol A Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)C(O)C(O)C3(CO)C(O)CC21C VKJLHZZPVLQJKG-UHFFFAOYSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 125000003563 glycoside group Chemical group 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
Description
【発明の詳細な説明】
本発明はギムネマ・シルベスタ乾燥葉から有効成分抽出
物を製造する方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an extract of active ingredients from dried leaves of Gymnema sylvestre.
従来の技術
ギムネマ・シルベスタ(Gymnesa、 5ylve
stre R。Conventional technology Gymnesa, 5ylve.
stre R.
Br、−以後GSと云う)はインド、中国、東南アジア
、アフリカ等の熱帯、亜熱帯に自生するカガイモ科に属
する植物であり、その温水抽出物は甘味抑制効果がある
ことが知られている一方、腸管における糖の吸収抑制効
果のあることも判り、薬用及び低カロリー飲食物への利
用が可能である。Br, - hereafter referred to as GS) is a plant belonging to the family Asclepiadaceae that grows naturally in tropical and subtropical countries such as India, China, Southeast Asia, and Africa, and its hot water extract is known to have a sweet taste suppressing effect. It has also been found to have the effect of inhibiting sugar absorption in the intestinal tract, and can be used for medicinal purposes and low-calorie foods and drinks.
GSの甘味抑制効果及び腸管における糖の吸収抑制効果
を示す有効成分はギムネマ酸と云われ。The active ingredient in GS that exhibits the sweet taste suppressing effect and the sugar absorption suppressing effect in the intestinal tract is called gymnemic acid.
ギムネマ酸はA1−A4の同族体があり、その基本構造
は、トリテルペンでるギムネマゲニン(3β、16β、
21β、22α、23.28−ヘキサヒドロキシオシア
ン−12エン)の誘導体とD−グルクロン酸から成る配
糖体である。又トリテルペンの水酸基は種々の有機酸で
エステル化されており、有機酸の種類はそれぞれの同族
体で異っている。Gymnemic acid has homologues A1-A4, and its basic structure is that of gymnemagenin (3β, 16β,
It is a glycoside consisting of a derivative of (21β, 22α, 23.28-hexahydroxyocyan-12ene) and D-glucuronic acid. Furthermore, the hydroxyl group of triterpenes is esterified with various organic acids, and the types of organic acids differ for each homolog.
伝統的にGSを用いているインドでは乾燥葉自体を食用
しており、糖尿病及びその他の病気の治療生薬として用
いられている。しかしその薬効成分はGSS水油抽出物
あると論じられており、恐らく上記ギムネマ酸を含む複
合成分の相互作用によると考えられている。In India, where GS is traditionally used, the dried leaves themselves are eaten and used as a herbal medicine to treat diabetes and other diseases. However, it has been argued that the medicinal component lies in the GSS water-oil extract, and it is thought that this is probably due to the interaction of the complex components including the above-mentioned gymnemic acid.
GS乾燥葉はインド等の自生地で採集され、天日乾燥の
後麻袋等に袋詰され流通されているが2自生地からの採
集品は夾雑物が多く、又長期間保存及び保管状態の不良
さから品質は一定でなく。GS dried leaves are collected from their natural habitat in India and other countries, and after drying in the sun, they are packaged in jute bags and distributed. Quality is inconsistent due to defects.
さらに通常衛生的管理下におかれていないため微生物に
汚染されているものが多く、特に土壌細菌や芽胞菌が多
く見られる。従って乾燥葉自体の使用は勿論、加工にも
幾多の問題がある。Furthermore, because they are not normally kept under sanitary control, they are often contaminated with microorganisms, especially soil bacteria and spore bacteria. Therefore, there are many problems not only in using the dried leaves themselves but also in processing them.
又植物の乾燥巣故、有効成分を抽出する場合も温度等の
抽出条件によっては有効成分に影響を及ぼす恐れがあり
、又GS水抽出液を濃縮して製剤化しようとしても、G
S水抽出液は発泡性がきわめて強く濃縮が困難なことも
あって、GSの有効成分を抽出して製剤化する方法は未
だ確立されていない。Furthermore, due to the dry nest of plants, when extracting the active ingredients, there is a risk that the extraction conditions such as temperature may affect the active ingredients.
Since the S aqueous extract has extremely strong foaming properties and is difficult to concentrate, a method for extracting the active ingredients of GS and formulating it into a formulation has not yet been established.
発 が 決しようと る8、 、
本発明は、GS乾燥葉から有効成分に影響を及ぼすこと
なく効率的に有効成分を抽出し、しかも粉体化したり他
成分と配合するなど、製剤が容易な程度に濃縮された無
菌抽出物を製造する方法を提供することを目的とする。8. The present invention efficiently extracts the active ingredient from dried GS leaves without affecting the active ingredient, and also enables easy formulation by pulverizing it or blending it with other ingredients. The object of the present invention is to provide a method for producing a highly concentrated sterile extract.
発明の構成
本発明に係るギムネマ・シルベスタ有効成分抽出物の製
造法は、ギムネマ・シルベスタ乾燥葉を濃度60%以上
の含水エタノールと温度30℃〜70℃で接触させて溶
出物質を抽出し、固液分離し、得られた抽出液を40〜
70℃で減圧濃縮して溶質濃度10%以りとすることを
特徴とする。Structure of the Invention The method for producing the active ingredient extract of Gymnema sylvestre according to the present invention involves contacting dried leaves of Gymnema sylvestre with aqueous ethanol with a concentration of 60% or more at a temperature of 30°C to 70°C to extract the eluted substances, and extracting the eluted substances. Separate the liquid and add the resulting extract to 40~
It is characterized in that it is concentrated under reduced pressure at 70°C to a solute concentration of 10% or more.
GSはつる性植物で、汀通高木にからみついて成育して
おり、GS!i!!を採集する際、ffi栖及び枝の一
部も混合されるので、GS乾燥葉とはGS巣に少量の葉
柄及び枝が混合した乾燥物を云う。GS is a climbing plant that grows entwined with Takagi Takagi, and GS! i! ! When collecting ffi and some of the branches, GS dried leaves refer to dried GS nests mixed with a small amount of petioles and branches.
GSの抽出に当って濃度60%以上の含水エタノールを
用いることにより、GS有効成分抽出率の向上、抽出物
の無菌化及び抽出液濃縮時の発泡抑制を同時に達成する
ことができる。By using aqueous ethanol with a concentration of 60% or more when extracting GS, it is possible to simultaneously improve the extraction rate of GS active ingredients, sterilize the extract, and suppress foaming when concentrating the extract.
即ちエタノール60%以上、望ましくは60〜80%の
含水エタノールを用い、30℃〜701でGS乾燥葉を
接触処理することにより抽出物から微生物は検出されず
、温水抽出より抽出効率は向上し、又濃縮に当って発泡
を抑制することができる。抽出成分をTLCで確認した
ところ温水抽出物と同様のスポットが確認された。That is, by contact-treating GS dried leaves at 30°C to 701°C using 60% or more ethanol, preferably 60 to 80% aqueous ethanol, no microorganisms are detected in the extract, and the extraction efficiency is improved compared to hot water extraction. Also, foaming can be suppressed during concentration. When the extracted components were confirmed by TLC, spots similar to those of the hot water extract were confirmed.
なお含水エタノール抽出に当り、GS乾燥葉を細断又は
粉砕することにより抽i効率をさらに向とさせることが
出来る。Note that during extraction with water-containing ethanol, the extraction efficiency can be further improved by shredding or crushing the dried GS leaves.
含水エタノール抽出液はペーパーフィルター等の精密濾
過により乾燥菓歿渣及び夾雑物と固液分離する。得られ
た抽出液は植物由来であり熱により変化する恐れがある
ので、40〜70℃で減圧濃縮する。The water-containing ethanol extract is separated into solid and liquid from dried confectionery residue and impurities by precision filtration using a paper filter or the like. Since the obtained extract is derived from plants and may change due to heat, it is concentrated under reduced pressure at 40 to 70°C.
抽出液を濃縮する場合、GS温水抽出液は発泡性が極め
て強く抽出液の濃縮は困難である。しかじ含水エタノー
ル抽出液においてはエタノール部分の蒸発過程では発泡
は少なく、エタノールが蒸発した後、水部分の蒸発が主
体になる段階で初めてで発泡が強くなる。従って水抽出
液に比べ含水エタノール抽出液の濃縮工程での発泡によ
る困難さは著しく軽減される。When concentrating the extract, the GS hot water extract has extremely strong foaming properties, making it difficult to concentrate the extract. In the water-containing ethanol extract, there is little foaming during the evaporation process of the ethanol portion, and the foaming only becomes strong after the ethanol has evaporated, at a stage when the water portion becomes the main evaporator. Therefore, the difficulty caused by foaming in the concentration process of a hydrous ethanol extract is significantly reduced compared to that of an aqueous extract.
即ち有効成分の抽出に当って、GS乾燥菓に対する含水
エタノールの濃度及び量比を適当に選ぶことにより、濃
縮工程でエタノール部分の蒸発のみにより溶質濃度を1
0%以上とすることが出来る。That is, when extracting the active ingredient, by appropriately selecting the concentration and amount ratio of aqueous ethanol to GS dried confectionery, the solute concentration can be reduced to 1 by only evaporating the ethanol portion in the concentration step.
It can be set to 0% or more.
濃縮後の溶1!!i濃度は高い程他成分との配合に便利
で又乾燥も容易になるが、“あまり高濃度にしようとす
るとエタノール部分が蒸発してしまい前記のように発泡
性が強くなるので、濃縮後の溶質濃度は10〜20%程
度とするのが適当である。Solution 1 after concentration! ! The higher the concentration, the more convenient it is to blend with other ingredients, and the easier drying will be. However, if you try to make the concentration too high, the ethanol portion will evaporate and the foaming properties will become stronger as mentioned above, so It is appropriate that the solute concentration is about 10 to 20%.
10%以上に濃縮された抽出液は、噴霧乾燥又は凍結乾
燥により乾燥して粉体とすることができる。噴霧乾燥す
る場合は有効成分が変質しないように乾燥雰囲気が70
℃以上にならないように注意する必要がある。The extract concentrated to 10% or more can be dried into a powder by spray drying or freeze drying. When spray drying, the drying atmosphere should be maintained at a temperature of 70% to prevent the active ingredients from deteriorating.
Care must be taken to ensure that the temperature does not exceed ℃.
1月
抽出剤として濃度60%以上の含水エタノールを使用す
ることにより、GS有効成分抽出率の向上、抽出物の無
菌化及び抽出液濃縮時の発泡抑制を同時に達成すること
ができ、また抽出及び濃縮温度を70℃以下にすること
によりGS有効成分の変質を防ぐことができる。By using aqueous ethanol with a concentration of 60% or more as an extractant, it is possible to simultaneously improve the extraction rate of GS active ingredients, sterilize the extract, and suppress foaming during extract concentration. By setting the concentration temperature to 70° C. or lower, deterioration of the GS active ingredient can be prevented.
実施例
GS乾燥51 kgをホモジナイザ〜にて裁断し99%
エタノール8見及び水4g、を加え30℃にて攪拌しつ
つ16時間接触させた後、ペーパーフィルターにより抽
出液と残渣を分離し、残液には再び99%エタノール8
文、水4ILを加え、30℃で攪拌しつつ16時間接触
させた後再びペーパーフィルターにて抽出液と残渣を分
離した0両者の抽出液を合せ40〜60℃にて減圧濃縮
し溶質濃度15%迄濃縮した。抽出成分をTLCで確認
したところ水抽出物と同様のスポットが確認された。Example: 51 kg of dried GS was cut with a homogenizer ~99%
After adding 8 g of ethanol and 4 g of water and keeping them in contact for 16 hours with stirring at 30°C, the extract and the residue were separated using a paper filter, and the remaining liquid was again mixed with 99% ethanol 8 g.
After adding 4 liters of water and keeping in contact for 16 hours with stirring at 30°C, the extract and residue were separated again using a paper filter. Both extracts were combined and concentrated under reduced pressure at 40 to 60°C to obtain a solute concentration of 15. %. When the extracted components were confirmed by TLC, spots similar to those of the water extract were confirmed.
この濃縮物を噴霧乾燥したところ水分5%の粉体330
gを得た。When this concentrate was spray-dried, a powder with a water content of 5% was obtained.
I got g.
比較例
GS乾燥葉1kgをホモジナイザーにて裁断し水12見
を加え50℃にて攪拌しつつ16時間接触させた後、ペ
ーパーフィルターにより抽出液と残渣を分離し、残渣に
は再び水12Jlを加え、5O℃で攪拌しつつ16時間
接触させた後再びペーパーフィルターにて抽出液と残液
を分離した0両者の抽出液を合せ40〜60℃にて減圧
濃縮しようとしたところ1発泡が次第に激しくなり濃縮
不可能であった・
え見立皇】
品質が不安定で、かつ微生物に汚染されていることが多
いギムネマ・シルベスタ乾燥菓から、有効成分のみを抽
出し、品質が安定した無菌抽出物を得ることができる。Comparative Example 1 kg of GS dried leaves was cut into pieces using a homogenizer, 12 liters of water was added, and the mixture was left in contact with the mixture for 16 hours while stirring at 50°C. The extract and the residue were separated using a paper filter, and 12 Jl of water was added to the residue again. After contacting for 16 hours with stirring at 50°C, the extract and residual liquid were separated again using a paper filter. When the two extracts were combined and concentrated under reduced pressure at 40 to 60°C, foaming gradually became more intense. It was impossible to concentrate. Emi Tatsuno] Only the active ingredients are extracted from Gymnema sylvestre dried confectionery, which is unstable in quality and often contaminated with microorganisms, resulting in a sterile extract with stable quality. can be obtained.
Claims (1)
タノールと温度30℃〜70℃で接触させて溶出物質を
抽出し、固液分離し、得られた抽出液を40〜70℃で
減圧濃縮して溶質濃度10%以上とすることを特徴とす
るギムネマ・シルベスタ有効成分抽出物の製造法。Dried leaves of Gymnema sylvesta are brought into contact with aqueous ethanol with a concentration of 60% or more at a temperature of 30°C to 70°C to extract eluted substances, followed by solid-liquid separation, and the resulting extract is concentrated under reduced pressure at 40 to 70°C. A method for producing an extract of active ingredients of Gymnema sylvestre, characterized in that the solute concentration is 10% or more.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61087128A JPS62246591A (en) | 1986-04-17 | 1986-04-17 | Production of extraction of active ingredient in gymnema-sylvestre r-br |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61087128A JPS62246591A (en) | 1986-04-17 | 1986-04-17 | Production of extraction of active ingredient in gymnema-sylvestre r-br |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS62246591A true JPS62246591A (en) | 1987-10-27 |
Family
ID=13906323
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP61087128A Pending JPS62246591A (en) | 1986-04-17 | 1986-04-17 | Production of extraction of active ingredient in gymnema-sylvestre r-br |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS62246591A (en) |
-
1986
- 1986-04-17 JP JP61087128A patent/JPS62246591A/en active Pending
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