JPS6219198Y2 - - Google Patents
Info
- Publication number
- JPS6219198Y2 JPS6219198Y2 JP1982034812U JP3481282U JPS6219198Y2 JP S6219198 Y2 JPS6219198 Y2 JP S6219198Y2 JP 1982034812 U JP1982034812 U JP 1982034812U JP 3481282 U JP3481282 U JP 3481282U JP S6219198 Y2 JPS6219198 Y2 JP S6219198Y2
- Authority
- JP
- Japan
- Prior art keywords
- container body
- polypropylene
- lid plate
- heat
- heat adhesive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000000844 anti-bacterial effect Effects 0.000 claims description 12
- 239000004743 Polypropylene Substances 0.000 claims description 11
- 239000012790 adhesive layer Substances 0.000 claims description 11
- -1 polypropylene Polymers 0.000 claims description 10
- 229920001155 polypropylene Polymers 0.000 claims description 10
- 229920001903 high density polyethylene Polymers 0.000 claims description 9
- 239000004700 high-density polyethylene Substances 0.000 claims description 9
- 229920003002 synthetic resin Polymers 0.000 claims description 6
- 239000000057 synthetic resin Substances 0.000 claims description 6
- 239000000853 adhesive Substances 0.000 claims description 4
- 230000001070 adhesive effect Effects 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims description 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 2
- 229920001971 elastomer Polymers 0.000 claims description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 2
- 239000011976 maleic acid Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005038 ethylene vinyl acetate Substances 0.000 claims 1
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 claims 1
- 229920005606 polypropylene copolymer Polymers 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 241000233866 Fungi Species 0.000 description 14
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UPZFLZYXYGBAPL-UHFFFAOYSA-N 2-ethyl-2-methyl-1,3-dioxolane Chemical group CCC1(C)OCCO1 UPZFLZYXYGBAPL-UHFFFAOYSA-N 0.000 description 1
- 229920002367 Polyisobutene Polymers 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 229920006163 vinyl copolymer Polymers 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
本考案は茸類その他の好気性菌類を培養するた
めの容器に関するものである。[Detailed Description of the Invention] The present invention relates to a container for culturing mushrooms and other aerobic fungi.
合成樹脂で作られ上面の開口周縁外側方に鍔を
設けた皿状の容器本体と内面に接着性層を有する
ふた板との組合せからなり、接着性層を鍔の上面
に接着することによつて容器本体をふた板で封止
するようにした培養用容器は特開昭52−145588号
公報,実公昭56−21274号公報などに開示されて
いるように広く知られている。これらはいずれも
容器本体に培地を入れ菌類を接種したときふた板
で封止して密閉状態とするものであり、容器本体
内部に外気が侵入しないので好気性菌類を培養す
ることはできない。また、培地を入れ菌類を接種
して封止したものを実験室に保管し或いは市場で
流通させることを目的としているので、一旦封止
したふた板を剥離したときこれを再び鍔に接着し
て密閉状態に復元することは全く考慮していな
い。従つて、接地のみを入れた容器体を密閉状態
で保管または流通させ、これに菌類を接種して再
び密閉するという使用法に供することができない
という不便さがある。 It consists of a dish-shaped container body made of synthetic resin with a flange on the outside of the opening on the top, and a lid plate with an adhesive layer on the inside. Culture containers in which the container body is sealed with a lid plate are widely known, as disclosed in Japanese Patent Application Laid-open No. 145588/1988, Japanese Utility Model Publication No. 21274/1983, and the like. In both of these containers, when a culture medium is placed in the container body and the fungi are inoculated, the container is sealed with a lid plate to create an airtight state, and since outside air does not enter the container body, aerobic fungi cannot be cultivated. In addition, the purpose is to store the culture medium, inoculate it with fungi, and seal it in the laboratory or distribute it in the market, so when the lid plate that has been sealed is peeled off, it can be glued back to the rim. Restoration to the sealed state was not considered at all. Therefore, there is an inconvenience in that it is not possible to store or distribute a container containing only a grounding material in a sealed state, inoculate it with fungi, and then seal it again.
一方、ふた板に防菌性フイルタで閉止した透孔
を設け、この透孔によつて菌類の接種と通気とが
行なえるようにした培養用容器が突公昭46−
37479号公報に開示されている。しかしながら、
このものは透孔から接種針を刺込んで接種を行な
うものであるため、菌類が培地に不均一に接種さ
れるのを避けられず、茸類の場合繁殖成長したと
きむらを生じて商品価値を低下させるという問題
がある。 On the other hand, a culture container was developed in 1977, in which the lid plate was provided with a through hole that was sealed with an antibacterial filter, allowing for inoculation of fungi and ventilation.
It is disclosed in Publication No. 37479. however,
Since this method inoculates by inserting an inoculating needle through a through hole, it is unavoidable that the fungi are inoculated unevenly into the culture medium, and in the case of mushrooms, when they reproduce and grow, it causes unevenness, which reduces the commercial value. There is a problem of lowering the
そこで本考案はこれらの問題点に鑑み、培地を
入れた容器本体のふた板を外して菌類を容易に均
一に接種し、再びふた板で密閉できるばかりか、
好気性菌類の培養に適する培養用容器を提供する
ものである。 Therefore, in view of these problems, the present invention not only allows the lid plate of the container containing the culture medium to be removed, fungi can be easily and evenly inoculated, and the lid plate is sealed again.
A culture container suitable for culturing aerobic fungi is provided.
即ち、本考案に係る培養用容器は、耐熱性およ
び熱接着性を有する合成樹脂で作られ開口周縁の
外側方に鍔が突設された容器本体と、再接着可能
な弱熱接着性層を内面に有しているとともに防菌
性フイルタによつて閉止された透孔が設けられて
いる耐熱性あるふた板とからなり、前記鍔の上面
に前記弱熱接着性層が熱接着されることによつて
前記ふた板が前記容器本体を封止するように溝成
されている。 That is, the culture container according to the present invention includes a container body made of a heat-resistant and heat-adhesive synthetic resin with a flange protruding outward from the periphery of the opening, and a weakly heat-adhesive layer that can be re-bonded. A heat-resistant lid plate is provided on the inner surface and has a through hole that is closed by an antibacterial filter, and the weak heat adhesive layer is thermally bonded to the upper surface of the flange. The lid plate is grooved so as to seal the container body.
次に、本考案の実施例を図面に基いて説明す
る。第1〜4図において、容器本体1はポリプロ
ピレン,高密度ポリエチレンなど耐熱性および熱
接着性を有する合成樹脂を用いて作られ厚さ30〜
500μ程度にして深さが1.0〜15mm程度のトレイ状
に形成されているとともに、その開口周縁2の外
側方に鍔3が一体に突出形成されている。また、
ふた板4はポリプロピレン,高密度ポリエチレ
ン,ポリエステル,ポリアミド,アルミニウム箔
などの単層体または複合体で厚さ5〜100μ程度
に作られ、耐熱性および好ましくは透明性を有し
ている。このふた板4は内面に厚さ0.5〜20μ程
度の耐熱性を有し再接着可能な弱熱接着性層5が
積層されているとともに適宜の透孔6が設けら
れ、この透孔6は内面側に接着した防菌性フイル
タ7によつて閉止されている。 Next, embodiments of the present invention will be described based on the drawings. In Figures 1 to 4, the container body 1 is made of a synthetic resin having heat resistance and heat adhesive properties, such as polypropylene or high-density polyethylene, and has a thickness of 30 mm to 30 mm.
It is formed into a tray shape with a thickness of about 500μ and a depth of about 1.0 to 15 mm, and a flange 3 is integrally formed to protrude outward from the opening periphery 2. Also,
The lid plate 4 is made of a single layer or a composite material of polypropylene, high density polyethylene, polyester, polyamide, aluminum foil, etc. and has a thickness of about 5 to 100 microns, and is heat resistant and preferably transparent. This lid plate 4 has a heat-resistant, re-adhesive weak heat adhesive layer 5 laminated with a thickness of approximately 0.5 to 20 μm on the inner surface, and is provided with appropriate through holes 6. It is closed by an antibacterial filter 7 glued to the side.
そして、ふた板4は鍔3に重ねて弱熱接着性層
5によつて熱接着し容器本体1を封止するもので
ある。 The lid plate 4 is stacked on the brim 3 and thermally bonded with a weak heat adhesive layer 5 to seal the container body 1.
第1,2図の実施例はふた板4に複数個の円形
の透孔6を設けてこれらを一枚の防菌性フイルタ
7で閉止したものである。また、第3,4図の実
施例は容器本体1およびふた板4の外面にポリア
ミド,ポリカーボネートなどからなる高強度の合
成樹脂層8をそれぞれ積層して機械的強度を増大
させるとともに、ふた板4に複数個の溝形の透孔
6を設けてこれらを一枚の防菌性フイルタ7で閉
止したものである。 In the embodiment shown in FIGS. 1 and 2, a plurality of circular through holes 6 are provided in the lid plate 4 and these are closed with a single antibacterial filter 7. In addition, in the embodiment shown in FIGS. 3 and 4, high-strength synthetic resin layers 8 made of polyamide, polycarbonate, etc. are laminated on the outer surfaces of the container body 1 and the lid plate 4 to increase mechanical strength, and the lid plate 4 A plurality of groove-shaped through holes 6 are provided in the filter, and these are closed with a single antibacterial filter 7.
第5,6図はふた板4そのものを弱熱接着性層
5で形成した単層品とし、中央部に比較的大きな
一個の透孔6を設けてこれを防菌性フイルタ7で
閉止したものである。 In Figures 5 and 6, the cover plate 4 itself is a single-layered product made of a weakly heat-adhesive layer 5, and a relatively large through hole 6 is provided in the center, which is closed with an antibacterial filter 7. It is.
前記各実施例において、弱熱接着性層5は容器
本体1がポリプロピレンで作られているときは単
体の高密度ポリエチレン,ポリプロピレンのマレ
イン酸変性体,ポリプロピレンを5〜30重量%混
入したエチレン−酢酸ビニル共重合体のいずれか
で構成される。また、容器本体1が高密度ポリエ
チレンで作られているときはポリプロピレン,ポ
リイソブチレンやブタジエンなどのゴム系樹脂,
珪酸や硫酸カルシウムなどの無機物からなる熱接
着阻害剤を5〜50重量%混入した高密度ポリエチ
レンのいずれかで構成される。この組合せにより
ふた板4を鍔3から剥離したとき、再度熱接着に
より容器本体1を封止することができる。 In each of the above embodiments, when the container body 1 is made of polypropylene, the weak heat adhesive layer 5 is made of simple high-density polyethylene, polypropylene modified with maleic acid, or ethylene-acetic acid mixed with 5 to 30% by weight of polypropylene. Composed of either vinyl copolymer. In addition, when the container body 1 is made of high-density polyethylene, rubber resin such as polypropylene, polyisobutylene, or butadiene,
It is composed of either high-density polyethylene mixed with 5 to 50% by weight of a thermal adhesion inhibitor made of an inorganic substance such as silicic acid or calcium sulfate. With this combination, when the lid plate 4 is peeled off from the collar 3, the container body 1 can be sealed again by thermal bonding.
更に、前記各実施例において防菌性フイルタ7
は通気性および透湿性を有するが細菌類は透過さ
せないものであり、例えばポリプロピレンや高密
度ポリエチレンなどの耐熱性を有する合成樹脂繊
維で作つた不織布で構成される。 Furthermore, in each of the above embodiments, the antibacterial filter 7
It has air permeability and moisture permeability but is impermeable to bacteria, and is composed of a nonwoven fabric made of heat-resistant synthetic resin fibers such as polypropylene and high-density polyethylene.
更にまた、前記各実施例の培養用容器は、例え
ば容器本体1に茸栽培用の培地を入れふた板4を
鍔3に熱接着して封止し、レトルト,オートクレ
ーブ,蒸気などの滅菌手段で培地を滅菌し、冷却
後直ちに或いは保管または流通させた後にふた板
4の一部または全部を鍔3から剥離して茸菌を培
地に接種し、剥離したふた板4を再び鍔3に熱接
着して封止し一定期間培養して育成,繁殖を行な
わせ、適宜な時期にふた板4を剥離除去するので
ある。この間、透孔6および防菌性フイルタ7を
通つて空気が出入し、殊に外気は細菌類が防菌性
フイルタ7により除去されて容器本体1に入る。 Furthermore, in the culture vessels of the above embodiments, for example, a medium for cultivating mushrooms is placed in the vessel body 1, the cover plate 4 is heat-bonded to the flange 3 to seal it, the medium is sterilized by sterilization means such as retort, autoclave, steam, etc., and immediately after cooling or after storage or distribution, a part or all of the cover plate 4 is peeled off from the flange 3, the mushroom fungus is inoculated into the medium, the peeled cover plate 4 is heat-bonded back to the flange 3 to seal, and the medium is cultured for a certain period of time to allow the fungus to grow and multiply, and the cover plate 4 is peeled off and removed at an appropriate time. During this time, air enters and exits through the through holes 6 and the antibacterial filter 7, and in particular, bacteria from the outside air are removed by the antibacterial filter 7 before entering the vessel body 1.
以上のように、本考案の容器本体とふた板とは
耐熱性を有しているので高温滅菌時に変形して商
品価値を低下させ或いは再接着を困難ならしめる
という不都合を伴わないものである。また、容器
本体の鍔に再接着可能な弱熱接着性層でふた体を
熱接着して封止するようにしたので、培地を滅菌
状態で保存し適宜の時期に開いて菌類を接種し再
封止でき、きわめて利用範囲が広いばかりか、ふ
た板および鍔を破損することなく容易に剥離で
き、且つそのために完全な再封止ができるもので
ある。そして、ふた体は防菌性フイルタで閉止し
た透孔を有しているので茸その他の好気性菌類を
雑菌の侵入による汚染の心配なく良好に培養で
き、更にふた板を剥離して菌類を均一に接種しむ
らなく繁殖成長させることができるものである。 As described above, since the container body and lid plate of the present invention are heat resistant, they do not suffer from deformation during high temperature sterilization, reducing commercial value or making re-adhesion difficult. In addition, the lid is heat-bonded and sealed with a weak heat adhesive layer that can be re-adhered to the brim of the container body, so the culture medium can be stored in a sterile state and opened at an appropriate time to inoculate the fungus and re-inoculate. Not only can it be sealed and can be used in a wide range of applications, but it can also be easily peeled off without damaging the cover plate and collar, and it can therefore be completely resealed. The lid has a through hole that is sealed with an antibacterial filter, so mushrooms and other aerobic fungi can be cultured well without worrying about contamination due to invasion of bacteria, and the lid can be peeled off to uniformly spread the fungi. It can be inoculated into plants and allowed to reproduce and grow evenly.
第1図は本考案の第一の実施例の一部切除した
平面図、第2図は第1図のA−A線に沿う断面
図、第3図は第二の実施例の一部切除した平面
図、第4図は第3図のB−B線に沿う断面図、第
5図は第三の実施例の一部切除した平面図、第6
図は第5図のC−C線に沿う断面図である。
1……容器本体、2……開口周縁、3……鍔、
4……ふた板、5……弱熱接着性層、6……透
孔、7……防菌性フイルタ。
Fig. 1 is a partially cutaway plan view of the first embodiment of the present invention, Fig. 2 is a sectional view taken along line A-A in Fig. 1, and Fig. 3 is a partially cutaway view of the second embodiment. FIG. 4 is a sectional view taken along line B-B in FIG. 3, FIG. 5 is a partially cutaway plan view of the third embodiment, and FIG.
The figure is a sectional view taken along the line CC in FIG. 5. 1... Container body, 2... Opening rim, 3... Tsuba,
4...Lid plate, 5...Weak heat adhesive layer, 6...Through hole, 7... Antibacterial filter.
Claims (1)
られ開口周縁2の外側方に鍔3が突設された容
器本体1と、再接着可能な弱熱接着性層5を内
面に有しているとともに防菌性フイルタ7によ
つて閉止された透孔6が設けられている耐熱性
あるふた板4とからなり、前記鍔3の上面に前
記弱熱接着性層5が熱接着されることによつて
前記ふた板4が前記容器本体1を封止するよう
に構成した培養用容器。 (2) 容器本体1がポリプロピレンで作られ、弱熱
接着性層5が高密度ポリエチレン,ポリプロピ
レンのマレイン酸変性体,ポリプロピレン混入
のエチレン−酢酸ビニル共重合体のいずれかで
構成されている実用新案登録請求の範囲(1)の培
養用容器。 (3) 容器本体1が高密度ポリエチレンで作られ、
弱熱接着性層5がポリプロピレン,ゴム系樹
脂,無機物混入の高密度ポリエチレンのいずれ
かで構成されている実用新案登録請求の範囲(1)
の培養用容器。[Claims for Utility Model Registration] (1) A container body 1 made of synthetic resin with heat resistance and heat adhesive properties and having a flange 3 protruding from the outside of the opening rim 2, and a low heat adhesive that can be reattached. It consists of a heat-resistant lid plate 4 which has a transparent layer 5 on its inner surface and a through hole 6 that is closed by an antibacterial filter 7, and the upper surface of the flange 3 is bonded with the weak heat adhesive. The culture container is configured such that the lid plate 4 seals the container body 1 by thermally adhering the sexual layer 5. (2) A utility model in which the container body 1 is made of polypropylene, and the weak heat adhesive layer 5 is made of high-density polyethylene, maleic acid modified polypropylene, or ethylene-vinyl acetate copolymer mixed with polypropylene. Culture container according to registered claim (1). (3) The container body 1 is made of high-density polyethylene,
Claims for Utility Model Registration (1) Where the weak heat adhesive layer 5 is composed of polypropylene, rubber-based resin, or high-density polyethylene mixed with inorganic substances
culture container.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3481282U JPS58137099U (en) | 1982-03-12 | 1982-03-12 | Culture container |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3481282U JPS58137099U (en) | 1982-03-12 | 1982-03-12 | Culture container |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS58137099U JPS58137099U (en) | 1983-09-14 |
JPS6219198Y2 true JPS6219198Y2 (en) | 1987-05-16 |
Family
ID=30046304
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3481282U Granted JPS58137099U (en) | 1982-03-12 | 1982-03-12 | Culture container |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS58137099U (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52145588A (en) * | 1976-05-31 | 1977-12-03 | Toppan Printing Co Ltd | Bacteria inspection tools |
JPS5621274U (en) * | 1979-07-26 | 1981-02-25 |
-
1982
- 1982-03-12 JP JP3481282U patent/JPS58137099U/en active Granted
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52145588A (en) * | 1976-05-31 | 1977-12-03 | Toppan Printing Co Ltd | Bacteria inspection tools |
JPS5621274U (en) * | 1979-07-26 | 1981-02-25 |
Also Published As
Publication number | Publication date |
---|---|
JPS58137099U (en) | 1983-09-14 |
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