JPH08289663A - Culture of mycelium of mushroom - Google Patents

Abstract

PURPOSE: To efficiently culture mycelium of mushroom, making an expensive aseptic room unnecessary and simply carrying out culture under general farm operation environment by covering a container, etc., in which a culture medium for culturing mushroom mycelia is contained with a covering material and treating the material under specific conditions. CONSTITUTION: A container or bag bodies 1 and 1' in which a culture medium 6 for culturing mushroom mycelia is contained are covered with a covering material 10 such as water repellent paper and intervals 11 and 12 are provided between the container or the bag bodies 1 and 1' and the covering material 10. Thereby, contamination with various germs is prevented while ensuring necessary oxygen in the initial stage of culture, and the primary hypha of the mushroom mycelium is cultured and the mycelia are spread into the culture medium 6, and further, culture of the secondary hypha is carried out.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、キノコ菌糸体の培養に
用いられる培地を入れた容器類又は袋体を、一重又は多
層に被覆する菌糸体の培養方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for culturing mycelium in which a container or bag containing a medium used for culturing mushroom mycelium is coated in a single layer or multiple layers.

【０００２】[0002]

【従来の技術】一般に、キノコの菌糸体の培養は下記の
ように行なわれている。先ずポリエチレン製、ポリプロ
ピレン製等、透明又は半透明の合成樹脂製袋に培地を充
填し、該袋の開口部に除菌フィルター等を被着する。次
にこれらを殺菌し、冷却する。更に除菌フィルター等を
取り外して培地に種菌を接種し、該除菌フィルター等を
再び被着する。そして培養室で培養する。2. Description of the Related Art Generally, mushroom mycelium is cultured as follows. First, a transparent or translucent synthetic resin bag made of polyethylene, polypropylene or the like is filled with a medium, and an opening of the bag is covered with a sterilization filter or the like. These are then sterilized and cooled. Further, the sterilization filter and the like are removed, and the medium is inoculated with inoculum, and the sterilization filter and the like are recoated. And it cultures in a culture room.

【０００３】上記除菌フィルター等の被着は、例えば紙
製の除菌フィルター、硬質プラスチック製の筒状体及び
上面に複数の孔が穿設された硬質プラスチック製のキャ
ップを用い、袋の開口部の周縁外周に筒状体を通して、
該周縁を筒状体の内側から外側へと折り返し、該開口部
に除菌フィルターを被せ、折り返した袋の周縁及び除菌
フィルターの周縁を挟んだ状態で、筒状体の外周にキャ
ップを押圧嵌合することにより行なわれている。The disinfection filter or the like is attached by using, for example, a disinfection filter made of paper, a cylindrical body made of hard plastic, and a cap made of hard plastic having a plurality of holes formed on the upper surface, and an opening of the bag. Pass the cylindrical body around the periphery of the part,
The periphery is folded back from the inside to the outside of the tubular body, the opening is covered with a sterilization filter, and the cap is pressed against the outer periphery of the tubular body while sandwiching the periphery of the folded bag and the periphery of the sterilization filter. It is done by fitting.

【０００４】ところが、上記のように従来法には、実際
のところ、培養中に培地に雑菌が蔓延し、不良品になっ
てしまうことがしばしば発生するという欠点がある。特
にかかる雑菌の蔓延は、キノコ菌糸体が優勢になるまで
の初期の培養段階において問題であり、通常又は悪環境
下では３〜１５％程度が不良品になってしまう。培地へ
の種菌の接種は相応に菌管理がなされた接種室で行なわ
れ、又相応に菌管理がなされた培養室で行なわれるが、
実際には双方ともに完全な無菌室というわけではなく、
室内には相当量の雑菌が浮遊している。However, as described above, the conventional method has a drawback that, in fact, various bacteria often infiltrate the medium during the culture, resulting in defective products. In particular, such infestation of various bacteria is a problem in the initial culture stage until the mushroom mycelium becomes dominant, and about 3 to 15% becomes a defective product under normal or adverse environment. Inoculation of the inoculum into the medium is carried out in an inoculation room where the bacteria are controlled accordingly, and in a culture room where the bacteria are controlled accordingly.
Actually, both are not completely sterile rooms,
A lot of miscellaneous bacteria are floating in the room.

【０００５】一方、培地を充填した容器類及び袋体の筒
状体とキャップとの間に挟み込んだ、合成樹脂製袋体の
周縁や除菌フィルターの周縁には必然的に雑多なしわが
生じていて、かかるしわに起因して双方の周縁の間に約
０．１〜１．０mmの小間隙が生じるのを避けられない。
従って、無菌管理下で行なわないと、雑菌が蔓延してし
まう為無菌環境下の接種室及び培養室を設置することが
必要となり、著しい経済的負担がかかる。On the other hand, various wrinkles are inevitably formed on the periphery of the synthetic resin bag and the periphery of the sterilization filter sandwiched between the cylindrical body of the container and the bag filled with the medium and the cap. Therefore, it is unavoidable that a small gap of about 0.1 to 1.0 mm is generated between both edges due to such wrinkles.
Therefore, if not performed under aseptic control, various bacteria will spread and it will be necessary to set up an inoculation room and a culture room in a sterile environment, which imposes a significant economic burden.

【０００６】[0006]

【発明が解決しようとする課題】本発明が解決しようと
する課題は、従来のキノコ菌糸体の培養では、その培養
中に、とりわけ初期の培養段階に雑菌が蔓延して不良品
になってしまうことがしばしば発生する点であり、又、
かかる雑菌の蔓延を防止しようとすると、著しい経済負
担がかかる点である。従って、本発明は、経済的に有利
でしかも簡便な方法で、キノコの菌糸体の培養、特にそ
の初期の培養段階における雑菌の汚染を防止するための
手段を提供しようとするものである。The problem to be solved by the present invention is that, in the conventional cultivation of mushroom mycelium, various bacteria prevail during the cultivation, especially in the initial cultivation stage, and become defective products. Is a point that often occurs, and
An attempt to prevent the spread of such bacteria will impose a significant economic burden. Therefore, the present invention aims to provide a means for preventing the contamination of various fungi in the cultivation of mushroom mycelium, especially in the initial cultivation stage thereof, by an economically advantageous and convenient method.

【０００７】[0007]

【課題を解決するための手段】上記の課題を解決するた
め、本発明はキノコ菌糸体の培養用培地を入れた容器類
又は袋体中でキノコ菌糸体の一次菌糸を培養して該培地
中に菌糸体を蔓延させ、さらに二次菌糸の培養を行う培
養方法において、一次菌糸の培養の少なくとも前半にお
いて前記容器類又は袋体を被覆材料により被覆し、且つ
該容器類又は袋体と該被覆材との間に間隔を設けること
により培養初期に必要な酸素を確保しながら雑菌の汚染
を防止することを特徴とする方法を提供する。Means for Solving the Problems In order to solve the above problems, the present invention cultivates the primary mycelium of mushroom mycelium in a container or bag containing a culture medium for mushroom mycelium, In a culturing method in which a mycelium is infested with a bacterium, and a secondary mycelium is further cultured, the container or bag is coated with a coating material in at least the first half of the culture of the primary mycelium, and the container or bag and the coating Provided is a method for preventing contamination of various bacteria while securing oxygen required at the initial stage of culture by providing a space between the material and the material.

【０００８】[0008]

【具体的な説明】キノコの菌糸体の培養においては、ま
ず培地を容器類又は袋体に入れて種菌し、冷却後種菌を
接種し、そして好気体条件下で菌糸の培養を行う。この
場合菌を接種した後、菌糸が培地のほぼ全体に拡がる間
の菌糸を一次菌糸と称する。一次菌糸の培養期間は通常
２５〜３０日間である。一次菌糸はさらに培養して二次
菌糸として熟成させる。この過程において、一次菌糸の
培養中、特にその前半（１０〜１５日間）においては培
地が雑菌により汚染されやすい。[Detailed Description] In culturing mycelium of mushrooms, first, a medium is put in a container or a bag to inoculate, the seed is inoculated after cooling, and the mycelia is cultured under aerobic conditions. In this case, after inoculation with the bacterium, the hyphae during the time when the hyphae spread over almost the entire medium are called primary hyphae. The culture period of primary hyphae is usually 25 to 30 days. The primary hyphae are further cultured and aged as secondary hyphae. In this process, the medium is likely to be contaminated with various bacteria during the culture of the primary hyphae, especially in the first half (10 to 15 days).

【０００９】そこで、本発明の方法においては、一次菌
糸の培養期間、特にその前半の０〜１５日間にわたって
前記容器類又は袋体を一重又は二重以上の多重に被覆す
ることにより雑菌による汚染を防止する。一次菌糸の培
養期間、特にその前半においては菌糸の増殖のための酸
素の必要量は比較的少いが、必須である。そのため、本
発明においては前記容器類又は袋体と前記被覆材料との
間には間隔を設け、空気を保持する。この間隔の容積
は、培養初期の酸素を十分に確保する見地から、当該被
覆材料中に収容される培地の体積と同程度か又はそれよ
り大きいことが好ましい。Therefore, in the method of the present invention, contamination of various bacteria is achieved by coating the above-mentioned containers or bags in a single layer or in a double layer or more in the culture period of the primary hyphae, particularly in the first half of the period of 0 to 15 days. To prevent. During the culture period of the primary hyphae, particularly in the first half thereof, the oxygen requirement for mycelial growth is relatively small but essential. Therefore, in the present invention, a space is provided between the container or bag and the coating material to retain air. The volume of this interval is preferably the same as or larger than the volume of the medium contained in the coating material, from the viewpoint of sufficiently securing oxygen in the initial stage of culture.

【００１０】上記の構成により、培養初期に必要な酸素
が十分に確保され且つ雑菌による培地の汚染が防止され
る。上記の期間の経過後、前記被覆材料は取り除く。従
って、本発明の方法においては、二次菌糸の培養期間中
は被覆材料は用いず、また場合によっては一次菌糸の培
養の後半においても被覆材料を用いない。但し、被覆材
料がスパンボンド不織布のごとく通気性の高いものであ
る場合は、外部から酸素が十分に供給されるので、一次
菌糸の培養の後半、そしてさらには二次菌糸の培養の期
間にわたって被覆材料により容器類又は袋体を覆っても
よい。被覆材料を取り除いた後は、気象条件の影響を受
け、菌糸の増殖、熟成が促進される。With the above construction, oxygen necessary for the initial stage of culture is sufficiently secured and contamination of the medium by various bacteria is prevented. After the above period has elapsed, the coating material is removed. Therefore, in the method of the present invention, the coating material is not used during the culture period of the secondary hyphae, and in some cases, the coating material is not used even in the latter half of the culture of the primary hyphae. However, when the coating material is highly breathable like spunbonded nonwoven fabric, sufficient oxygen is supplied from the outside, so the coating is performed during the latter half of the culture of the primary mycelium, and further during the culture of the secondary mycelium. The container or bag may be covered with a material. After removing the coating material, the growth and ripening of mycelia are promoted under the influence of weather conditions.

【００１１】なお、被覆は、容器類又は袋体を１個づつ
行う必要はなく、例えば、培養棚、プラスチック、バケ
ット等に複数の容器類又は袋体を並べ、１組の被覆材料
により被覆してもよい。本発明においては、被覆材料は
酸素消費の少ない培養の初期においてのみ用いられるの
で、被覆材料に通気性等特定の要件は必要でなく、任意
の材料を用いることができる。すなわち、本発明に用い
る被覆材は、紙・フィルム・不織布・織物・編物・プラ
スチック、木材、陶器、金属などが用いられ、形状、材
質など特に限定されない。しかし、形状としては、培地
の上部又は全体を、被覆し、容器類又は袋体との間隙を
培地容積と同程度有するようにすることである。被覆材
で被覆した時、開口部は、折り曲げてホチキス止め、輪
ゴム止め、又はアルミ板などを開口部に当てがい２〜４
回折りたたんで両端を任意に曲げ密封する方法、又は、
接着剤、熱シーラー、粘着テープなどで密封する方法な
どが用いられる。It is not necessary to coat the containers or bags one by one. For example, a plurality of containers or bags are arranged on a culture shelf, plastic, bucket, etc. and coated with a set of coating materials. May be. In the present invention, since the coating material is used only in the initial stage of culture in which oxygen consumption is low, the coating material does not need to have specific requirements such as air permeability, and any material can be used. That is, the coating material used in the present invention may be paper, film, non-woven fabric, woven fabric, knitted fabric, plastic, wood, pottery, metal, etc., and the shape and material are not particularly limited. However, the shape is such that the upper part or the whole of the culture medium is covered so that the space between the culture medium and the container or the bag has the same extent as the culture medium volume. When covered with a covering material, bend the opening and stap it, apply a rubber band, or apply an aluminum plate to the opening 2-4
A method of folding and sealing both ends arbitrarily by folding, or
A method of sealing with an adhesive, a heat sealer, an adhesive tape or the like is used.

【００１２】本発明に用いる被覆材の紙は、撥水紙、耐
油紙、合成紙、殺菌紙などが好ましく用いられる。しか
し遮蔽環境下などでは、クラフト紙、コピー用紙なども
用いられる。被覆材のフィルムは、ポリエチレン、ポリ
プロピレンなどのオレフィン系フィルム、ポリアミドフ
ィルム、ポリスチレンフィルムなどの単一又は複合フィ
ルム及びクレー、炭酸カルシウム、などの微粉末を混入
させた微多孔フィルムなどが用いられる。As the paper of the covering material used in the present invention, water repellent paper, oil resistant paper, synthetic paper, sterilized paper and the like are preferably used. However, under shielded environments, kraft paper and copy paper are also used. As the film of the covering material, an olefin film such as polyethylene or polypropylene, a single or composite film such as a polyamide film or a polystyrene film, and a microporous film in which fine powder such as clay or calcium carbonate is mixed is used.

【００１３】被覆材の不織布は、スパンボンド法、メル
トブロー法、トウ開繊法、サーマルボンド法、乾式短繊
維法等の単一方式或は二種以上の方式を組合せて作られ
る。不織布に用いられる繊維としては、ポリプロピレ
ン、ポリエチレン、ポリエステル等の疎水性繊維が好ま
しく用いられる。更に、被覆材の疎水性繊維からなる織
編物、プラスチックポット等のプラスチック成形品、皮
・木材・竹等の箱、陶器、金属などの容器などが用いら
れる。中でも、通気性、遮光性等の点から不織布を被覆
材として用いることが好ましい。The non-woven fabric of the covering material is made by a single method such as a spun bond method, a melt blow method, a tow opening method, a thermal bond method, a dry short fiber method, or a combination of two or more methods. As the fibers used for the non-woven fabric, hydrophobic fibers such as polypropylene, polyethylene and polyester are preferably used. Further, a woven or knitted material made of a hydrophobic fiber as a covering material, a plastic molded product such as a plastic pot, a box of leather, wood, bamboo or the like, a pottery, a container of metal or the like is used. Above all, it is preferable to use a non-woven fabric as a covering material from the viewpoints of air permeability, light shielding property, and the like.

【００１４】容器類の形状、材質としては、キノコ菌の
生育生理に適した培地を保持できるものであれば特に素
材、形状ともに限定されない。素材としては、ポリエチ
レン、ポリプロピレン等のポリオレフィン、ポリアミ
ド、ポリエステル等がある。又、袋体としては、ポリエ
チレン製、ポリプロピレン製等の透明又は半透明のフィ
ルムから成る袋状物である。更に袋体の開口部に硬質プ
ラスチック製の筒状体及び上面に複数の孔が穿設された
硬質キャップを取り付けて用いるか、袋体の上部一部分
に該フィルター材を取りつけ用いられる。形状は、丸
形、四角型、長方形、等大きさ、形は特に限定されない
が、袋体の酸素ガス、炭素ガス等の通気を良くすること
が好ましい。The shape and material of the containers are not particularly limited as long as they can hold a medium suitable for the growth physiology of mushroom fungi. Examples of the material include polyolefin such as polyethylene and polypropylene, polyamide, polyester and the like. The bag body is a bag-shaped product made of a transparent or translucent film made of polyethylene, polypropylene or the like. Further, a tubular body made of hard plastic and a hard cap having a plurality of holes formed in the upper surface are attached to the opening of the bag body, or the filter material is attached to a part of the upper portion of the bag body. The shape is not particularly limited, such as round shape, square shape, rectangular shape, and the like, but it is preferable to improve the ventilation of oxygen gas, carbon gas, etc. of the bag.

【００１５】菌糸体の培養方法 次に、本発明の培養方法について説明する。本発明のキ
ノコ菌糸体を培養する培地としては、一般的に使用され
ているものが使用できる。例えば、米糠、ふすま、コー
ン粉末、おがくず及び市販の栄養源の混合物に対して６
０〜７０重量％程度の水を混合したものが使用できる。 Culturing method of mycelium Next, the culturing method of the present invention will be described. As a medium for culturing the mushroom mycelium of the present invention, a commonly used medium can be used. For example, 6 for a mixture of rice bran, bran, corn flour, sawdust and commercial sources of nutrition.
A mixture of about 0 to 70% by weight of water can be used.

【００１６】本発明の培養方法を具体的に示す。代表的
な培養方法の一例を図１に示す。袋体１に培地６を充填
し、本発明のフィルター材２を、袋体開口部１′に取り
付けた、筒状体３の上部円周に沿って、例えば９cm角の
フィルター材２を筒状体の円を中心に被せ、キャップ４
で正しく嵌合し、殺菌工程、冷却工程を経る。この時該
フィルター材は、筒状体とキャップの間に挟み込んだ
時、シワなどを起こさせないように被着させることが好
ましい。というのは、殺菌後の戻し空気又は冷却中の雑
菌の浸入を阻止を良好に行う為に重要である。The culturing method of the present invention will be specifically described. An example of a typical culture method is shown in FIG. The bag body 1 is filled with the medium 6, and the filter material 2 of the present invention is attached to the bag body opening portion 1 ′. Along the upper circumference of the tubular body 3, for example, a 9 cm square filter material 2 is tubular. Put the body circle on the center, cap 4
Correctly fit, and go through the sterilization process and cooling process. At this time, the filter material is preferably applied so as not to cause wrinkles when sandwiched between the tubular body and the cap. This is important in order to effectively prevent the invasion of returned air after sterilization or other bacteria during cooling.

【００１７】次いで無菌環境下で、袋体１の開口部１′
に取り付けたキャップ４と、フィルター材２を取り外
し、培地６に種菌を接種した後、再び本発明のフィルタ
ー材２を被せてキャップ４を押圧嵌合させ、密閉する。
更に、得られた図１の容器類又は袋体を図２に示す様に
被覆材で一重に被覆して培養するか、あるいは図３に示
すように、被覆材を二重以上に被覆して培養する。図２
においては第１の被覆材１０が設けられている。この被
覆１１と袋体１との間には空気が入った間隙１１，１２
が設けられている。例えば口の閉じ部にはアルミ板が設
けられており、１４は袋を構成するためのシール部を示
す。図３において、１５は第２の被覆を示す。Next, in an aseptic environment, the opening 1'of the bag 1 is
After removing the cap 4 and the filter material 2 attached to the medium and inoculating the medium 6 with the inoculum, the filter material 2 of the present invention is covered again, and the cap 4 is press-fitted and sealed.
Further, the obtained container or bag shown in FIG. 1 is coated with a coating material as shown in FIG. 2 for culturing, or as shown in FIG. Incubate. Figure 2
In, a first coating material 10 is provided. Air gaps 11 and 12 between the cover 11 and the bag 1
Is provided. For example, an aluminum plate is provided at the mouth closing portion, and 14 indicates a seal portion for forming a bag. In FIG. 3, 15 indicates a second coating.

【００１８】[0018]

【発明の効果】本発明の各種被覆材を用いた菌糸体の培
養方法は、キノコ菌糸体の培養中に於けるとりわけ初期
段階に雑菌汚染防止でき、高価な無菌室が不要となり簡
単に培養ができる。具体的には、原木栽培を行っている
遮蔽下の場所や遮蔽下の一般的な農作業環境下でも培養
ができる。EFFECT OF THE INVENTION The mycelium culture method using various coating materials of the present invention can prevent contamination of various bacteria, especially at an early stage during the cultivation of mushroom mycelia, and eliminate the need for an expensive sterile room for easy culture. it can. Specifically, the cultivation can be performed even in a place where the logs are cultivated under a shield or in a general agricultural work environment under a shield.

【００１９】[0019]

【実施例】次に、実施例により本発明を具体的に説明す
るが本発明の範囲はこれらの実施例に限定されるもので
ない。実施例 合成樹脂製袋体 ；内容２Ｌのポリプロピレン製袋体
（厚み３０μ） 培地 ；米糠５部（重量部以下同じ）、ふ
すま部１０部、おがくず８５部、水６５部を混合したも
の、１．２kg培地。 培地の殺菌及び冷却；常圧流通蒸気（温度９８℃）で３
時間殺菌後２０℃で放冷 種菌の種類 ；しいたけ菌 筒状体及びキャップ；硬質プラスチック製 培養 ；温度２２±４℃、湿度４０〜７０
％ 培養日数 ；１５日間、加温及び加湿無菌的管
理なしEXAMPLES Next, the present invention will be specifically described by way of examples, but the scope of the present invention is not limited to these examples. Example Synthetic resin bag: Polypropylene bag having a content of 2 L (thickness 30 μ) Medium: Rice bran 5 parts (the same applies hereafter by weight), bran part 10 parts, sawdust 85 parts, water 65 parts 1. 2 kg medium. Sterilization and cooling of the medium; 3 with atmospheric pressure steam (temperature 98 ° C)
After being sterilized for 20 hours, it is allowed to cool at 20 ° C. Type of inoculum; Shiitake tube and cap; Hard plastic culture; Temperature 22 ± 4 ° C, humidity 40-70
% Culture days: 15 days, no warming and humidification sterile control

【００２０】実施例１〜７ 袋体に培地を充填し、袋体開口部に取り付けた筒状体の
上部円周に沿って、９cm角の不織布フィルター材を筒状
体の円を中心に被せ、キャップで正しく嵌合し、殺菌工
程、冷却工程を経る。次いで、無菌環境下で袋体の開口
部に取りつけたキャップとフィルター材を取り外し、培
地に種菌を接種した後、再びフィルター材を被せてキャ
ップを押圧嵌合させ、密閉する。 Examples 1 to 7 A bag was filled with a medium, and a non-woven fabric filter material of 9 cm square was covered around the circle of the tube along the upper circumference of the tube attached to the opening of the bag. , Fit it correctly with the cap, and go through the sterilization process and cooling process. Then, in a sterile environment, the cap and the filter material attached to the opening of the bag are removed, the medium is inoculated with the inoculum, the filter material is again covered, and the cap is press-fitted and sealed.

【００２１】該袋体の全体を各種被覆材で図２の様に一
重被覆し、各々１００ケ用意し、１５日間屋内、及び屋
外で一次培養して、雑菌汚染状態をそれぞれ評価して、
第１表に示した。The entire bag was coated with various coating materials as shown in FIG. 2, 100 pieces of each were prepared, and primary culture was carried out indoors and outdoors for 15 days to evaluate the contamination of various bacteria.
The results are shown in Table 1.

【００２２】実施例８〜９ 実施例１〜７と同様の上記袋体の全体を、図３の様にク
ラフト紙、及び織物で被覆し、更に、その上からポリエ
チレンフィルムで二重被覆したものを各々１００ケ用意
し、１５日間屋内、及び屋外で一次培養して、雑菌汚染
状態をそれぞれ評価して第１表に示した。 Examples 8 to 9 The same bag as in Examples 1 to 7 was entirely coated with kraft paper and a fabric as shown in FIG. 3, and then double-coated with a polyethylene film. 100 each of them were prepared and subjected to primary culture indoors and outdoors for 15 days, and the contamination state of various bacteria was evaluated and shown in Table 1.

【００２３】比較例１ 実施例１〜７と同様にした上記袋体だけ（被覆材を使用
しない場合）１００ケ用意し、１５日間屋内、及び屋外
で一次培養して、雑菌汚染状態をそれぞれ評価して第１
表に示した。COMPARATIVE EXAMPLE 1 100 bags of the above-mentioned bags similar to those of Examples 1 to 7 (when no covering material is used) were prepared and subjected to primary culture indoors and outdoors for 15 days to evaluate the contamination of various bacteria. Then first
Shown in the table.

【００２４】実施例１０ 実施例１の状態で、更に、９０日間屋内で培養した。そ
の結果、培地を観察したところ、全てのきのこの菌糸体
が優勢且つ良好に培養できた。 Example 10 In the state of Example 1, the cells were further cultured indoors for 90 days. As a result, when the medium was observed, all mycelia of the mushrooms were predominantly and satisfactorily cultured.

【００２５】実施例１１ 実施例８の初期の培養した後、被覆材を取り、培養袋体
の状態で、更に屋内で９０日間培養した。その結果培地
を観察したところ、全てのきのこの菌糸体が優勢且つ良
好に、培養できた。 Example 11 After the initial culture of Example 8, the coating material was removed, and the culture bag was further cultured indoors for 90 days. As a result, when the medium was observed, all mycelium of the mushrooms could be cultivated predominantly and satisfactorily.

【００２６】実施例１２ 実施例１と同様の培養袋体を１００ケ作り、３段から成
る培養棚に並べた。この培養棚全体を、通気性を有する
ポリプロピレン不織布（目付８０ｇ／ｍ² ）の被覆材で
被覆させ、該被覆材との間に間隔を設けるようにして、
不透明なビニールシートで更に二重被覆させて１５日間
培養した。その結果、培地を観察したところ全てのきの
この菌糸体が優勢且つ良好に培養できた。 Example 12 100 culture bags similar to those in Example 1 were prepared and placed on a culture shelf composed of 3 stages. The whole culture shelf is covered with a covering material of air-permeable polypropylene non-woven fabric (weight per unit area: 80 g / m ² ) so that a space is provided between the covering and the covering material.
It was further double-coated with an opaque vinyl sheet and cultured for 15 days. As a result, when the medium was observed, all mushroom mycelia could be predominantly and satisfactorily cultured.

【００２７】[0027]

【表１】 [Table 1]

【図面の簡単な説明】[Brief description of drawings]

【図１】図１は袋体の側面図である。FIG. 1 is a side view of a bag body.

【図２】図２は被覆材を一重被覆した側面図である。FIG. 2 is a side view showing a single coating of a coating material.

【図３】図３は被覆材を二重被覆した側面図である。FIG. 3 is a side view in which a coating material is double coated.

【符号の説明】[Explanation of symbols]

１，１′…合成樹脂フィルムから成る袋体 ２…不織布から成るフィルター材 ３…合成樹脂製筒状体 ４…合成樹脂製キャップ ５…合成樹脂製キャップの開孔部 ６…培地 １０…被覆材 １１，１２…袋体と被覆材の間隙 １３…アルミ板 １４…被覆材のシール部 １５…２枚目の被覆材 1, 1 '... Bag made of synthetic resin film 2 ... Filter material made of non-woven fabric 3 ... Synthetic resin cylindrical body 4 ... Synthetic resin cap 5 ... Synthetic resin cap opening 6 ... Medium 10 ... Coating material 11, 12 ... Gap between bag and covering material 13 ... Aluminum plate 14 ... Sealing portion of covering material 15 ... Second covering material

Claims (1)

【特許請求の範囲】[Claims] 【請求項１】 キノコ菌糸体の培養用培地を入れた容器
類又は袋体中でキノコ菌糸体の一次菌糸を培養して該培
地中に菌糸体を蔓延させ、さらに二次菌糸の培養を行う
培養方法において、一次菌糸の培養の少なくとも前半に
おいて前記容器類又は袋体を、被覆材料により被覆し、
且つ該容器類又は袋体と該被覆材との間に間隔を設ける
ことにより培養初期に必要な酸素を確保しながら雑菌の
汚染を防止することを特徴とする方法。1. A primary mycelium of a mushroom mycelium is cultivated in a container or a bag containing a culture medium for mushroom mycelium, the mycelium is spread in the medium, and a secondary mycelium is further cultured. In the culturing method, in at least the first half of the culture of primary mycelia, the containers or bags are coated with a coating material,
In addition, a method is provided, in which contamination is prevented by securing an oxygen required in the initial stage of culture by providing a space between the container or bag and the covering material.