JPS60244287A - Preparation of substrate for producing cellulase - Google Patents
Preparation of substrate for producing cellulaseInfo
- Publication number
- JPS60244287A JPS60244287A JP10224684A JP10224684A JPS60244287A JP S60244287 A JPS60244287 A JP S60244287A JP 10224684 A JP10224684 A JP 10224684A JP 10224684 A JP10224684 A JP 10224684A JP S60244287 A JPS60244287 A JP S60244287A
- Authority
- JP
- Japan
- Prior art keywords
- hypochlorous acid
- cellulase
- bagasse
- treated
- alkali
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Enzymes And Modification Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明はセルラーゼ（セルロース加水分解酵素）生産用
基質の製造法、更に詳しくは、セルロース系バイオマス
資源（以下、単にバイオマスと称することがある）上次
亜塩素酸系化合物で処理することを％黴とするセルラー
ゼ生産用基質の製造法に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing a substrate for cellulase (cellulose hydrolase) production, and more specifically, a method for producing a substrate for cellulase (cellulose hydrolase) production, and more specifically, a method for producing a substrate for cellulase (cellulose hydrolase) production, and more specifically, a hypochlorous acid compound on cellulose biomass resources (hereinafter sometimes simply referred to as biomass). This invention relates to a method for producing a substrate for cellulase production, which involves treating the mold with % mold.
近年、セルロース系バイオマス資源上酵素分解によシ糖
化してその構成単位であるグルコース、キシロースとな
し、更にこれを発酵して得られるエタノール全液体燃料
もしくは化学原料として利用することが検討されている
。然し、バイオマスにセルロースの結晶構造やりダニ７
等の夾雑物のためにそのままでは糖化されに（い性質の
ものである。そこで、バイオマスをボールミルやロール
ミル等で機械的に破砕する方法、アルカリ、カドキセン
等の化学薬剤による処理ｔ−施す方法、あるいはア七ト
ン、エタノール等の有機溶媒によ多処理する方法等によ
り前処理が行われている。一方、このように前処理され
たバイオマス全セルラーゼ生産用基質として利用しよう
とすると、純セルロースを基質に用いた場合に比べて充
分なセルラーゼ生産性が得られないという欠点があった
。このため、従来より純セルロースが基質として用いら
れているが、この方法はセルラーゼ生産性は高いものの
、高価格でアク、セルラーゼ生産コストの大部分を占め
てしまうという難点があった０本発明は、斯かる実状に
おいて、セルロース系バイオマスのセルラーゼ生産用炭
素源への応用について鋭意研究を行った結果、バイオマ
スを次亜塩素酸系化合物で処理すると、セルラーゼの生
産性が著しく向上し、かつセルラーゼ全簡易かつ安価に
製造できることを見出し、本発明を完成した。In recent years, consideration has been given to saccharifying cellulosic biomass resources through enzymatic decomposition to produce its constituent units, glucose and xylose, and then fermenting the resulting ethanol to use it as an all-liquid fuel or chemical raw material. . However, the crystalline structure of cellulose and mites in biomass7
Due to impurities such as, biomass is difficult to be saccharified as it is.Therefore, there are methods of mechanically crushing biomass with a ball mill or roll mill, methods of treating it with chemical agents such as alkali and cadoxene, etc. Alternatively, pretreatment is carried out by multiple treatments with organic solvents such as acetone, ethanol, etc. On the other hand, when attempting to use the biomass pretreated in this way as a substrate for whole cellulase production, pure cellulose is This method has the disadvantage that sufficient cellulase productivity cannot be obtained compared to when using it as a substrate.For this reason, pure cellulose has traditionally been used as a substrate, but although this method has high cellulase productivity, Under these circumstances, the present invention was developed as a result of intensive research into the application of cellulosic biomass as a carbon source for cellulase production. The present invention has been completed based on the discovery that the productivity of cellulase is significantly improved and cellulase can be produced easily and inexpensively by treating it with a hypochlorous acid compound.
すなわち本発明に、セルロース系バイオマス資源ケ次亜
塩素酸系化合物で処理することを特徴とするセルラーゼ
生産用基質の製造法葡提供するものである。That is, the present invention provides a method for producing a substrate for cellulase production, which comprises treating a cellulosic biomass resource with a hypochlorous acid compound.
本発明で使用されるバイオマスとしては、特に制限はな
く、例えば通常入手可能なバガス等を挙げることができ
る。バイオマスは、乾燥後、１０メツシユ以下に粉砕し
て使用するのが好ましい。The biomass used in the present invention is not particularly limited, and examples thereof include commonly available bagasse and the like. It is preferable to use the biomass after drying and pulverizing it into 10 meshes or less.
また、更にこれは予めアルカリ処理などの化学的ｉσ処
理を行なって２くのが好ましい。Furthermore, it is preferable to perform a chemical iσ treatment such as an alkali treatment in advance.
本発明で使用される次亜塩素酸系化合物としては、例え
ばＣａ （Ｃ１Ｏ）２、ＮａＣ１０２ＫＣ１Ｏ等の次亜
塩素酸のアルカリ金塊塩又はアルカリ土類金属塩等の０
５〜２０重量％（以下、単にチで示す〕、好ましくに３
〜５％水浴液が挙げられる。ノ（イオマスは、該水溶液
中に２〜２０チ、好ましくは５〜ｌＯチ配合される。Examples of hypochlorous acid compounds used in the present invention include alkali gold bullion salts or alkaline earth metal salts of hypochlorous acid such as Ca (C1O)2 and NaC102KC1O.
5 to 20% by weight (hereinafter simply referred to as H), preferably 3
-5% water bath solution. 2 to 20 iomas, preferably 5 to 10 iomas, is blended into the aqueous solution.
本発明の製造法は、例えば次亜塩素酸系化合物中にバイ
オマスを懸濁し、５〜５０℃、好ましくは２０〜３０’
Ｃで３０分以上、好ましくは１〜３時間攪拌処理を行な
ったのち、Ｆ液がｐＨ７付近となるまで充分水洗してか
らケーキ’＆Ｆ取することにより実施される。In the production method of the present invention, for example, biomass is suspended in a hypochlorous acid compound, and
After stirring with C for 30 minutes or more, preferably 1 to 3 hours, the solution is thoroughly washed with water until the pH of the solution F reaches around 7, and then the cake '&F is removed.
叙上の如く、本発明によればバイオマスを有効に利用で
きる定め安価に、しかも操作上の煩雑さもなく短時間に
セルラーゼ生産用基質全製造することができる。As described above, according to the present invention, the entire substrate for cellulase production can be produced in a short period of time, at a low cost, and without any operational complexity, while making effective use of biomass.
次に実施例全挙げて本発明を説明する。Next, the present invention will be explained with reference to all examples.
実施例１
１０メツシユ以下に粉砕したバガスを、１％ＮａＯＨ溶
液中で１２００にて３０分間蒸煮処理し、残液全戸数・
水洗してアルカリ処理バガスを得た０次いでこのアルカ
リ処理バガスを乾燥重量の１０′倍量の水に懸濁し、次
いでその３％相肖量のＣａＣＣＢＤ）ｔをｍえ、１時間
室温にてときどき攪拌しながら処理した。その後水洗・
濾過τ（り返し、洗浄後のｐＨが７前後となった時点で
ケーキを回収してアルカリ−次亜塩素酸処理バガスを得
た。Example 1 Bagasse crushed into 10 meshes or less was steamed in a 1% NaOH solution at 1200℃ for 30 minutes, and the remaining liquid was
The alkali-treated bagasse was washed with water to obtain an alkali-treated bagasse.Then, the alkali-treated bagasse was suspended in 10 times its dry weight of water, and then 3% of CaCCBD) was added thereto for 1 hour at room temperature. Processed with stirring. Then wash with water.
Filtration τ (repeated) When the pH after washing became around 7, the cake was collected to obtain alkali-hypochlorous acid treated bagasse.
斯（シて得られたアルカリ処理バガス、アルカリ−次亜
塩素酸処理バガス、及びＭＡ科バガスの成分全分析した
結果を第１表に示す。なお、分析結果は乾燥試料中のセ
ルロース含量、ヘミセルロース含量及びリグニン含量全
百分率で示した。Table 1 shows the results of a complete analysis of the components of the alkali-treated bagasse, alkali-hypochlorous acid-treated bagasse, and MA family bagasse thus obtained. It is expressed as a total percentage of lignin content and lignin content.
第 １ 表
第１表より、次亜塩素酸処理によって原料バガス中のヘ
ミセルロース及びリグニンが著Ｌ＜減少していることが
認められる。Table 1 From Table 1, it is recognized that the hemicellulose and lignin in the raw bagasse were significantly reduced by the hypochlorous acid treatment.
次に原料バガス、アルカリ処理バガス及びアルカリ−次
亜塩素酸処理バガスを用いてセルラーゼの生産培養試験
、及び糖化試験を行なった。Next, a cellulase production culture test and a saccharification test were conducted using the raw bagasse, alkali-treated bagasse, and alkali-hypochlorous acid-treated bagasse.
しセルラーゼの生産培養試験〕
下記組成の液体培地に公知のセルラーゼ生産菌であるト
リコデルマ・リーセイＱ Ｍ ９４１４ （ＡＴＣＣ２
６９２１）’１ｋＷａｌして２８−１＝Ｉ Ｃ（Ｄ温度
で７日間培養した。この培養液（以下、酵素液と称する
）のセルラーゼ活性、カルボキシメチルセルロース（Ｃ
ＭＣ）分解活性、β−グルコシダーゼ活性及び可溶性た
んぼ（量を下記方法によシ測定した。その結果を第２表
に示す。Cellulase production culture test] Trichoderma reesei Q M 9414 (ATCC2
6921)'1kWal and cultured at 28-1=IC (D temperature) for 7 days.The cellulase activity of this culture solution (hereinafter referred to as enzyme solution), carboxymethylcellulose (C
MC) decomposition activity, β-glucosidase activity, and soluble paddy (MC) amount were measured by the following methods. The results are shown in Table 2.
培地の組成：
炭素源 ３０り
ＫＨ２ＰＯ４−４９
（ＮＨ４）２ＳＯ４４ｙ
尿素 １タ
ポリペプトン ０．２１
ＣａＣｔ２０．３ ｆ
ＭｔＳＯ＜ ・７Ｌ０ ０．３ ｔ
ＦｅＳＯａ ” ７Ｈ２０５ｔ’？
Ｍｎ５Ｏ６−７Ｈ７０１，６Ｗ
ｚｎＳ０４６７Ｈ２０１，４’９
ＣＯＣｔ２・６Ｈ２０２，０ツ
ＴＩＷｅｅｎ ８０ １ ｆ
蒸留水 １０００ｍ
ｐＨｓ、 ｓ
セルラーゼ活性：
酵素液のＰ紙に対する分解労金もってセルラーゼ活性（
以下、ＦＰＡと称する）を評価した。すなわち、Ｆ紙（
ワラトモン凰１、ｌ ×６ ｃｍ ） ５０Ｗｋ基質と
し、これに酵素液０．５ ｍｌ！と０．０５ Ｍりエン
酸緩衝液（ＰＨ４，８） １．０−を加え、５０Ｃにて
６０分間酵素反応を行なった後、直ちにジニトロサリチ
ル酸試薬３．０１−刀ｎえ、１００℃にて５分間加熱し
発色させる。次いで水１６ｍ１′に加え、５５Ｑｎｒｒ
ｚ７）波長で比色定量して還元力をめた。Composition of the medium: Carbon source 30 liters KH2PO4-49 (NH4)2SO44y Urea 1 Tapolypeptone 0.21 CaCt20.3 f MtSO<・7L0 0.3 t FeSOa "7H205t'? Mn5O6-7H701,6W znS0467H201,4 '9 COCt2・6H202,0 TIWeen 80 1 f Distilled water 1000 m pHs, s Cellulase activity: Cellulase activity (
Hereinafter referred to as FPA) was evaluated. In other words, F paper (
1, l x 6 cm) 50Wk substrate and 0.5 ml of enzyme solution! After adding 0.05 M phosphoric acid buffer (PH4,8) 1.0- and enzymatic reaction at 50C for 60 minutes, immediately add dinitrosalicylic acid reagent 3.0-1 and at 100°C. Heat for 5 minutes to develop color. Then add 16ml of water and add 55Qnrr
z7) Reducing power was determined by colorimetric determination using wavelength.
ＦＰＡ活性は、１分間に工μｍｏｔのグルコースに相当
する還元力を生成する酵素活性ｔ■年単位したＯ
ＣＭＣ分解活性：
ＣＭＣｔ−１％濃度溶解した０、 ０５ Ｍクエン酸緩
衝液（ＩＪＨ４，８） ０．５＋ｄＫ酵素液０．５ｄ’
に７ＪＤえ５゜Ｃにて３０分間酵素反応を行った後、以
下ＦＰＡ測定時と同様の操作によ、９ＣＭＣ活性を測定
した。FPA activity is defined as the enzyme activity that produces a reducing power equivalent to μmot of glucose per minute. ) 0.5+dK enzyme solution 0.5d'
After enzymatic reaction was carried out for 30 minutes at 5°C in 7JD, 9CMC activity was measured using the same procedure as in FPA measurement.
ＣＭＣ分解活性単位は、１分間にＬｚｚｍｏｔのグルコ
ースに相当する還元力にあたる酵素活性’ｋｌ単位とし
た。The CMC decomposition activity unit was the enzyme activity 'kl unit, which corresponds to the reducing power equivalent to Lzzmot glucose per minute.
β−グルコシダーゼ活性：
５ｍＭのバラニトロフェニルβ−Ｄグルコピラノシド溶
液０．５ ｍｔｉ基質とし、これに０．２ Ｍ酢酸緩衝
液（ｐＨ５，０） ’ｋ １． Ｏ−及び酵素液０．１
ｍｇ’１７１ｇえ、５０℃にて２０分間酵素反応を行な
った後、生成したパラニトロフェノール’に４００ｎｍ
の波長で吸光度全測定した。β−グルコシダーゼ活性４
１分間に１μｍ□ｔのパラニトロフェノールを生成する
酵素量全１単位とした。β-Glucosidase activity: 0.5 mti substrate was 5 mM varanitrophenyl β-D glucopyranoside solution, and 0.2 M acetate buffer (pH 5,0)'k 1. O- and enzyme solution 0.1
After enzymatic reaction at 50°C for 20 minutes, the generated para-nitrophenol was exposed to 400 nm.
The total absorbance was measured at the wavelength of . β-glucosidase activity 4
The total amount of enzyme to produce 1 μm□t of para-nitrophenol per minute was 1 unit.
可溶性たんばく量：
酵素液中の可溶性たんばく量を、牛血清アルブミンを標
準とするローリ−法により定量した。Amount of soluble protein: The amount of soluble protein in the enzyme solution was determined by the Lowry method using bovine serum albumin as a standard.
第２表
第２表から明らかな如（、アルカリ処理後に次亜塩素酸
処理することによシ、セルラーゼをはじめ可溶性たんば
くの生産量の増大が認められた。As is clear from Table 2, an increase in the production of soluble proteins including cellulase was observed by treating with hypochlorous acid after alkali treatment.
１０〇−容三角フラスコにアルカリ処理バガス又はアル
カリ−次亜塩素酸処理バガスを乾燥重量にして５ｖ入れ
、Ｆ’ＰＡが７５ Ｕ／＋−の市販セルラーゼ（セルラ
ーゼ・アマノＴＡＰ−６）の０．０５ Ｍりエン酸緩衝
液溶液（ｐＨ４，８’）５０°−を加え、５０℃にて４
８時間、１３５ストロークの往復振とり機で攪拌しなが
ら糖化反応を行ない、生成するグルコースを高速液体ク
ロマトグラフィーにて定量した。その結果、アルカリ処
理バガス及びアルカリ・次亜塩素酸処理バガスの各基質
５ｆからグルコースがそれぞれ３．Ｏｆ及び２．９５９
が生成した。5 v of alkali-treated bagasse or alkali-hypochlorous acid-treated bagasse (dry weight) was placed in a 100-capacity Erlenmeyer flask, and 0.5 v of commercially available cellulase (Cellulase Amano TAP-6) with an F'PA of 75 U/+- was added. Add 50°-05M phosphate buffer solution (pH 4,8') and incubate at 50°C for 4 hours.
The saccharification reaction was carried out for 8 hours while stirring with a 135-stroke reciprocating shaker, and the produced glucose was quantified by high performance liquid chromatography. As a result, 3.0% glucose was obtained from each substrate 5f of alkali-treated bagasse and alkali/hypochlorous acid-treated bagasse. Of and 2.959
was generated.
この結果から本発明による処理バガスはセルラーゼ生成
用基質として優れたものであシ、糖化に対してはアルカ
リ処理バガスと同等の効果を奏することが明らかとなっ
た。These results revealed that the bagasse treated according to the present invention is excellent as a substrate for cellulase production, and has the same effect on saccharification as the bagasse treated with alkali.
実施例２
前記第２表に示す組成の液体培地において、炭素源とし
てアルカリ処理バガス又はアルカリ・次亜塩素酸処理バ
ガスをセルロース濃度が３％となるよう゛に加えた以外
は同組成の培地２．５ｔを５を容発酵槽に入れ、これに
公知のセルラーゼ生産菌であシ当出願人の保存するトリ
コデルマ属の変異株随６２７株の種培養液を４％添加し
、ｐＨ４，０〜５．４及び溶存酸素量ｔ−２０〜５０％
となるように制御しながら２８Ｃにて７日間培養してセ
ルラーゼの生産性を実施例１と同様にして調べた。その
′結果を第３表に示す。Example 2 Medium 2 with the same composition as shown in Table 2 except that alkali-treated bagasse or alkali/hypochlorous acid-treated bagasse was added as a carbon source so that the cellulose concentration was 3%. .5 t was placed in a 5-volume fermenter, and 4% of the seed culture of Trichoderma mutant strain No. 627, a known cellulase-producing bacterium preserved by the applicant, was added thereto, and the pH was adjusted to 4.0 to 5. .4 and dissolved oxygen amount t-20~50%
Cellulase productivity was examined in the same manner as in Example 1 by culturing at 28C for 7 days while controlling the conditions such that: The results are shown in Table 3.
第３表
第３表より、アルカリ処理バガスに比べて本発明による
処理バガスの方がセルラーゼ生産性が高いことがわかる
。Table 3 Table 3 shows that the bagasse treated according to the present invention has higher cellulase productivity than the bagasse treated with alkali.
更に、使用炭素源全セルロース製置が５チとなるように
加えた以外は上記と同様にして培養を行なったところ第
４表に示す結果を得た。Furthermore, cultivation was carried out in the same manner as above except that 5 pieces of total cellulose were added as a carbon source, and the results shown in Table 4 were obtained.
第４表
Ｍ４表に示す如く、アルカリ処理バガスを基質とした培
養でに菌の生育阻害によフセルラーゼがほとんど生産さ
れないのに対し、本発明（よる処理バガスでは何らの阻
害を受けることなくセルラーゼが生産され、本発明方法
によ）製造されたセルラーゼ生産用基質は優れた効果を
奏する。As shown in Table 4, Table M4, in the culture using alkali-treated bagasse as a substrate, almost no fucellulase is produced due to bacterial growth inhibition, whereas in the bagasse treated with the present invention, cellulase is produced without any inhibition. The substrate for cellulase production produced by the method of the present invention exhibits excellent effects.
実施例３゜
沖縄産原料バガス’１ｒ１０メツシュ以下に粉砕後、ア
ルカリ処理を施さずにそのまま乾燥重量の１０倍Ｉの水
に懸濁し、更にその３チ相当量のＣａ（ＣｔＯ）を金入
れ、ときどき攪拌しながら３０分間処理し、以下実施例
１と同様に水洗・濾過をくシ返したのちケーキを回収し
た。この１回次亜塩素酸処理バガスの１ｍを再度上記の
操作に付してケーキを回収し２回次亜塩素酸処理バガス
とした。次にこの１回次亜塩素酸処理バガスと２回次亜
塩素戚処理バガスｔ−実施例五のセルラーゼ生産培養試
験と同様にしてセルラーゼの生産性を調べた。その結果
全第５表に示す。Example 3 Raw bagasse from Okinawa was crushed to a size of 1r10 mesh or less, then suspended in water with an amount of 10 times its dry weight without alkali treatment. The mixture was treated for 30 minutes with occasional stirring, and the cake was recovered after repeated washing and filtration in the same manner as in Example 1. 1 m of this once-treated bagasse with hypochlorous acid was subjected to the above-mentioned operation again, and a cake was recovered to obtain bagasse treated with hypochlorous acid twice. Next, cellulase productivity was investigated in the same manner as in the cellulase production culture test of Example 5 for the once-hypochlorous acid-treated bagasse and twice-hypochlorite-treated bagasse T. The results are shown in Table 5.
第５表
ｍ５表から明らかな如く、本発明の次亜塩素酸カルシウ
ムにより処理した場合に、比較例の未処理バガスの場合
と比べ著しくセルラーゼ、可溶性たんぽ（の生産量が増
加してお）、また１回次亜塩素酸処理バガスよりは２回
次亜塩素酸処理ノくガス全円いた場合にセルラーゼ、可
溶性たんばくの生産量がさらに増加することが認められ
る。As is clear from Table 5, when treated with the calcium hypochlorite of the present invention, the production of cellulase and soluble dandelion significantly increased compared to the untreated bagasse of the comparative example. In addition, it is observed that the production of cellulase and soluble protein is further increased when the bagasse is treated with hypochlorous acid twice than when the bagasse is treated with hypochlorous acid once.
の代りにＮａＣＩＪ：Ｊ又ｕＫｃｔｏ、”を使用してア
ルカリ・次亜塩素酸バガスを得た。また、これとは別に
アルカリ処理を施さずに原料バガスｋ Ｃａ（ＣｔＯ）
ｚ、ＮａＣｔＯ又はＫＣｌ０だけで処理して次亜塩素酸
ノくガスを得た。これらについて実施例１と同様にして
セルラーゼの生産性を調べた結果を第６表に示すＯ第６
表
以上Alkali/hypochlorous acid bagasse was obtained by using NaCIJ: J or uKcto, instead of NaCIJ:J or uKcto.In addition, raw material bagasse kCa(CtO) was obtained without alkali treatment.
z, treated with NaCtO or KCl0 alone to obtain hypochlorous acid gas. The productivity of cellulase was investigated in the same manner as in Example 1, and the results are shown in Table 6.
More than a table
Claims (1)
物で処理することを特徴とするセルラーゼ生産用基質の
製造法。 ２ 次亜塩素酸系化合物が次亜塩素酸のアルカリ金属又
はアルカリ土類金属塩の０．５〜２０％水溶液である特
許請求の範囲第１項記載の製造法。 ３、 セルロース系バイオマスが、アルカリ処理すれた
ものである特許請求の範囲第１項又は第２項記載の製造
法。[Claims] 1. A method for producing a substrate for cellulase production, which comprises treating cellulosic biomass resources with a hypochlorous acid compound. 2. The production method according to claim 1, wherein the hypochlorous acid compound is a 0.5 to 20% aqueous solution of an alkali metal or alkaline earth metal salt of hypochlorous acid. 3. The production method according to claim 1 or 2, wherein the cellulosic biomass is alkali-treated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10224684A JPS60244287A (en) | 1984-05-21 | 1984-05-21 | Preparation of substrate for producing cellulase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10224684A JPS60244287A (en) | 1984-05-21 | 1984-05-21 | Preparation of substrate for producing cellulase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60244287A true JPS60244287A (en) | 1985-12-04 |
| JPH0533984B2 JPH0533984B2 (en) | 1993-05-20 |
Family
ID=14322250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10224684A Granted JPS60244287A (en) | 1984-05-21 | 1984-05-21 | Preparation of substrate for producing cellulase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60244287A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011041522A (en) | 2009-08-21 | 2011-03-03 | Asahi Breweries Ltd | METHOD FOR PRODUCING beta-GLUCANASE AND XYLANASE WITH WHEAT BRAN AND LIQUID CULTURE MEDIUM |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5058302A (en) * | 1973-09-17 | 1975-05-21 | ||
| JPS5076305A (en) * | 1973-09-20 | 1975-06-23 | ||
| JPS5381382A (en) * | 1976-12-18 | 1978-07-18 | Kiichirou Sarui | Method for treating peattmoss |
| JPS5396386A (en) * | 1977-01-29 | 1978-08-23 | Stanford Research Inst | Production of cellulase by thermophilic bacteria * thielavia terestris |
| JPS5639777A (en) * | 1979-09-11 | 1981-04-15 | Baiorisaac Center:Kk | Preparation of cellulase-producing koji |
| JPS58155088A (en) * | 1982-03-09 | 1983-09-14 | Rikagaku Kenkyusho | Novel cellulase and its preparation |
-
1984
- 1984-05-21 JP JP10224684A patent/JPS60244287A/en active Granted
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5058302A (en) * | 1973-09-17 | 1975-05-21 | ||
| JPS5076305A (en) * | 1973-09-20 | 1975-06-23 | ||
| JPS5381382A (en) * | 1976-12-18 | 1978-07-18 | Kiichirou Sarui | Method for treating peattmoss |
| JPS5396386A (en) * | 1977-01-29 | 1978-08-23 | Stanford Research Inst | Production of cellulase by thermophilic bacteria * thielavia terestris |
| JPS5639777A (en) * | 1979-09-11 | 1981-04-15 | Baiorisaac Center:Kk | Preparation of cellulase-producing koji |
| JPS58155088A (en) * | 1982-03-09 | 1983-09-14 | Rikagaku Kenkyusho | Novel cellulase and its preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0533984B2 (en) | 1993-05-20 |
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