JPS5930046A - Total bilirubin quantitative determination meter - Google Patents
Total bilirubin quantitative determination meterInfo
- Publication number
- JPS5930046A JPS5930046A JP14130182A JP14130182A JPS5930046A JP S5930046 A JPS5930046 A JP S5930046A JP 14130182 A JP14130182 A JP 14130182A JP 14130182 A JP14130182 A JP 14130182A JP S5930046 A JPS5930046 A JP S5930046A
- Authority
- JP
- Japan
- Prior art keywords
- light
- photoelectric conversion
- wavelength light
- total bilirubin
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、総ビリルビン値定量計に関するもので、異な
る波長光の光量測定を同時に行なうことを特徴とするも
のである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a total bilirubin value quantitative meter, which is characterized by simultaneously measuring the amount of light of different wavelengths.
一般に血清中の総ビリルビン測定は・血清よシなる被検
体に基準光源より光を照射し、４５５ｎｍ波長光の吸収
度を測定し、補正の為５７５ｎｍ波長光の吸収度の測定
を行なう。Generally, total bilirubin in serum is measured by: irradiating a sample such as serum with light from a reference light source, measuring the absorbance of 455 nm wavelength light, and then measuring the absorbance of 575 nm wavelength light for correction.
従来、第１図に示す如くこの測定方法は、暗箱４に入れ
られた測定される血清よりなる被検体６に、暗箱４に開
けられた***、５よシ基準光源１がらの光が集光ｌ／ン
ズ２によって、集光された光が当てられる。被検体６を
通過した光は、最初４５５ｎｍ波長光通過フィルター９
によって、４５５ｎｍ波長光のみが光電変換素子１′１
１．により電流で測定できる。次に同じ状態で、４５５
ｎｍ波長光通過フィルター９を交換して５７５ｎｍ波長
光通過フィルター１１に替えると、５７５ｎｍ波長光の
みが光′醒変換素子１Ｘによって電流変換される。Conventionally, as shown in FIG. 1, in this measurement method, light from a reference light source 1 is focused on a specimen 6 made of serum to be measured placed in a dark box 4 through a small hole 5 in the dark box 4. Condensed light is applied by the l/lens 2. The light that has passed through the object 6 is first passed through a 455 nm wavelength light passing filter 9.
Therefore, only the 455 nm wavelength light is transmitted to the photoelectric conversion element 1'1.
1. It can be measured by current. Next, in the same state, 455
When the nm wavelength light passing filter 9 is replaced with a 575 nm wavelength light passing filter 11, only the 575 nm wavelength light is converted into a current by the light conversion element 1X.
この２つの電流値の差が総ビリルビン量に対応するもの
である。ゼロ調整は被検体６の内容を純水まｆＣｅま空
気とし、前述の測定を行なうことによってなされる、
このような従来例においては、４５５ｎｍ波長光通過フ
ィルター９と５７５ｎｍ波長光通過フィルター１１を交
換して測定する為、同一条件下とばなり得す、別々に行
なう測定による誤差を生じる。The difference between these two current values corresponds to the total amount of bilirubin. Zero adjustment is performed by making the content of the object 6 pure water, fCe, or air, and performing the measurement described above. In such a conventional example, the 455 nm wavelength light passing filter 9 and the 575 nm wavelength light passing filter 11 are replaced. Since the measurements are made under the same conditions, errors may occur due to separate measurements.
本発明は、上記欠点を除去するもので、４５５ｎｍ波長
光と５７５ｎｍ波長光の吸収測定を同時に行なうことを
目的とする。The present invention aims to eliminate the above drawbacks, and aims to simultaneously perform absorption measurements of 455 nm wavelength light and 575 nm wavelength light.
以下、実施例を示す。Examples are shown below.
第２図は、本発明の総ビリルビン値定量組であるが、１
はハロゲンランプ等の長寿命の基準光源、６は血清より
ｉる被検体、８け４５５ｎｍ波長光を反射させ５７ ｓ
ｎｍ波長波長光過させる色フイルタ−，９は４５５ｎｍ
波長光通過フィルター、１１ （（Ｊ ５７５ ｎ ｍ
波長光通過フィルター、１＼。Figure 2 shows a total bilirubin value determination set according to the present invention.
6 is a long-life reference light source such as a halogen lamp, 6 is a sample of serum, and 8 is a 455 nm wavelength light reflected for 57 s.
Color filter that passes nm wavelength wavelength light, 9 is 455 nm
Wavelength light passing filter, 11 ((J 575 nm
Wavelength light passing filter, 1\.
１２は光電変換素子である。12 is a photoelectric conversion element.
次に測定法について説明する。基準光源１は暗箱４に開
けらｆ′１．；ｅ***５より被検体８に照射する。Next, the measurement method will be explained. The reference light source 1 is opened in the dark box 4 and is f'1. ;e Irradiate the subject 8 through the small hole 5.
被検体６を通過した光は、その光路−ヒに置かれた色フ
イルタ−８によｐ４５５ｎｍ波長光は反射し、５７５ｎ
ｍ波長光は通過する。このように測定波長光が分離する
ので、同一光による１回の測定が可能となる。次に反射
方向に置かれｆＣ，４５５ｎｍ波長光通過フィルター９
により、ｉ ４５５ ｎ ｍ波長光のみが選択され、後
に置かれた光電変換素子１′ｇ、によって４５５ｎｍ波
長光による電流が測定される。また通過方向にＩｄ、
５７５ ｎ ｍ波長光通過フィルター１１によＪｌｌ、
！５７５ｎｍ波長光のみが選択され、後に置かれた光電
変換素子１２によって５７５ｎｍ波長光による電流が測
定さり、る。The light that has passed through the object 6 is reflected by the color filter 8 placed in the optical path, and the 455 nm wavelength light is reflected, and the 575 nm wavelength light is reflected by the color filter 8 placed in the optical path.
m wavelength light passes through. Since the measurement wavelength lights are separated in this way, one measurement using the same light becomes possible. Next, an fC, 455 nm wavelength light passing filter 9 is placed in the reflection direction.
Accordingly, only the i 455 nm wavelength light is selected, and the photoelectric conversion element 1'g placed later measures the current due to the 455 nm wavelength light. Also, Id in the passing direction,
Jll by the 575 nm wavelength light passing filter 11,
! Only the 575 nm wavelength light is selected, and the photoelectric conversion element 12 placed later measures the current due to the 575 nm wavelength light.
次に、これらの電流値による足取解析を第３図により説
明する。ｉｎ、１１の例えば、フォト・ダイオード等の
光電変換素子により変換された前述の４５５ｎｍ波長光
による電流をＩＩ、５７５ｎｍ波長光による電流を工υ
とする。１３は抵抗Ｈａ、１５ｉｊ抵抗Ｒｂ、１４．１
６は対数演算素子、１７は差分演算素子の構成にすると
、出力ｅは次のようになる。Next, gait analysis using these current values will be explained with reference to FIG. For example, in, 11, the current due to the aforementioned 455 nm wavelength light converted by a photoelectric conversion element such as a photo diode, II, the current due to 575 nm wavelength light, υ
shall be. 13 is resistance Ha, 15ij resistance Rb, 14.1
When 6 is a logarithmic operation element and 17 is a difference operation element, the output e is as follows.
ｅ ＝ ａ ｌｏｇＲａ／工ａ −ｂ ｌｏｇＲ２ｌ／
Ｉ ｂ ・・−−−−（１）ａ、ｂは定数である。e = a logRa/Eq a -b logR2l/
Ib...---(1) a and b are constants.
次に、ゼロ調整により抵抗Ｒａ、Ｒｈを決Ｉ）る。Next, determine the resistances Ra and Rh by zero adjustment.
第２図では、被検体６の内容全純水または窒気に変えて
、同様の測定をする。第３図で、この時に光電変換素子
１＼、１２に流れる電流をＩａ’１１．、 Ｉ ｂ’１
１゜とし、ＲＩＬｆ、変えずにＲ５を変えて出力をゼロ
とすると、吹のようになる。In FIG. 2, the same measurement is performed except that the content of the subject 6 is changed to pure water or nitrogen. In FIG. 3, the current flowing through the photoelectric conversion elements 1\, 12 at this time is Ia'11. , I b'1
If the angle is set to 1° and the output is set to zero by changing R5 without changing RILf, the result will be as follows.
ａｌｏｇＲａ／Ｉａ＼−ｂ ｌｏｇ Ｒ１＋／Ｉ ｂ
ｏ ＝ ｏ −−−−” （Ｉｆ）このように決められ
た抵抗Ｒａ 、 Ｒｈによ、！７（１）式より（１１）
式を引くと、出力ｅは次のようになる。alogRa/Ia\-b log R1+/I b
o = o -----" (If) With the resistances Ra and Rh determined in this way, !7 From formula (1), (11)
Subtracting the formula, the output e is as follows.
ｅ ＝ ａ １０ｇ工ａＯ／Ｉａ−０１０ｇよりＯ５／
より−・−（ｉｉｔ）これがゼロに調整達れた出力であ
る。このように抵抗１３．１５のいずれかを可変抵抗と
することにより、可変抵抗のみを調整することでゼロ調
整が可能となる。e = a 10g engineering aO/Ia-010g O5/
From -.-(iit) This is the output that has reached zero adjustment. By making one of the resistors 13 and 15 a variable resistor in this way, zero adjustment becomes possible by adjusting only the variable resistor.
以上の実施例に基づく本発明は、回じ〈医療用の採血分
析器、科学機器等への応用も可能である。The present invention based on the above embodiments can also be applied to medical blood sampling analyzers, scientific instruments, etc.
本発明は、以上説明したように、分光ミラーを用いて、
２種のフィルタによる同時劇定が可能であり、従って通
過フィルター９，１１を取り替える作業を必要とせず、
それに伴う誤差を無くすことができ、又、２回行なって
いたゼロ調整が１回で済む等、すぐれた効果を有する。As explained above, the present invention uses a spectroscopic mirror to
Simultaneous determination using two types of filters is possible, so there is no need to replace the passing filters 9 and 11.
This has excellent effects, such as eliminating errors associated with this, and requiring only one zero adjustment instead of twice.
第１図は従来の実施例を示す。第２図は本発明の実施例
、第６図は総ビリルビン量検出回路例を示す。
１・・・基準電源 ２・・・集光レンズ４・・
・暗 箱 ５・・・小 穴６・・・被検体
８・・・分光ミラー９・・・４５５ｎｍ波長
光通過フィルター１’ｇ・・・光電変換素子
１１・・・５７５ｎｍ波長光通過フィルター１２・・・
光電変換素子 １３・・・固定抵抗１４．１６・・・
対数演算素子
１５・・・可変抵抗 １７・・・差分演算素子以
上
出願人 高島産業株式会社FIG. 1 shows a conventional embodiment. FIG. 2 shows an embodiment of the present invention, and FIG. 6 shows an example of a total bilirubin amount detection circuit. 1... Reference power supply 2... Condensing lens 4...
・Dark box 5...Small hole 6...Subject
8... Spectroscopic mirror 9... 455 nm wavelength light passing filter 1'g... Photoelectric conversion element 11... 575 nm wavelength light passing filter 12...
Photoelectric conversion element 13...Fixed resistance 14.16...
Logarithmic operation element 15...Variable resistor 17...Differential operation element or more Applicant Takashima Sangyo Co., Ltd.
Claims (1)
度を計測して、該血清中のビリルビン値。 を測定する総ビリルビン値定ｌ計において、被検体を通
過した光を分割する分光ミラーと、該分光ミラーにより
分割された光のうち所定の波長光のみの光量を検出する
手段とを設け、該光量検出手段は複数にて彦ることを特
徴とする総ビリルビン値定量計。[Claims] The bilirubin value in serum is determined by measuring the absorbance of light from a reference light source that has passed through a subject made of serum. A total bilirubin level meter for measuring total bilirubin level is provided with a spectroscopic mirror that splits the light that has passed through the subject, and a means for detecting the amount of light of only a predetermined wavelength out of the light that has been split by the spectroscopic mirror. A total bilirubin value quantitative meter characterized in that a plurality of light quantity detection means are used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14130182A JPS5930046A (en) | 1982-08-13 | 1982-08-13 | Total bilirubin quantitative determination meter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14130182A JPS5930046A (en) | 1982-08-13 | 1982-08-13 | Total bilirubin quantitative determination meter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5930046A true JPS5930046A (en) | 1984-02-17 |
Family
ID=15288697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14130182A Pending JPS5930046A (en) | 1982-08-13 | 1982-08-13 | Total bilirubin quantitative determination meter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5930046A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0189359U (en) * | 1987-12-02 | 1989-06-13 | ||
| EP0772021A2 (en) | 1995-10-30 | 1997-05-07 | Ishikawajima-Harima Jukogyo Kabushiki Kaisha | Cooling towers |
-
1982
- 1982-08-13 JP JP14130182A patent/JPS5930046A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0189359U (en) * | 1987-12-02 | 1989-06-13 | ||
| EP0772021A2 (en) | 1995-10-30 | 1997-05-07 | Ishikawajima-Harima Jukogyo Kabushiki Kaisha | Cooling towers |
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