JPS58116406A - Agricultural germicidal composition - Google Patents

Abstract

PURPOSE:The titled composition, containing kasugamycin mixed with a specific compound as active constituents, capable of exhibiting better synergistic effects on blast of rice plants and blight caused by Pseudomonas glumae than the single chemical and applicable to both raising boxes for seedlings and water surfaces. CONSTITUTION:An agricultural germicidal composition, containing kasugamycin or a salt thereof which is an antifungal substance produced by an actinomyces Streptomyces kasugaensis and a safe controlling agent against blast of rice plants for terrestrial and aerial application, exhibiting penetrating germicidal effects on the blast of rice plants, and having improved remedial effects and antimicrobial activity against Pseudomonas glumae paddy rice with low toxicity to humans, cattle, fishes and shellfishes without the phytotoxicity to rice plants and S-benzyl O,O-diisopropyl thiophosphate which has been put to practical use as a controlling agent against blast of rice plants at 1:(2.5-50) mixing weight ratio. The resultant composition is used by adding a carrier or adjuvant, etc. thereto, and formulating into a dosage form, e.g. wettable powder or dust.

Description

【発明の詳細な説明】 本発明は、カスガマイシンまたはその塩類（以下（−Ｋ
ＳＭ　Ｊという）とチオリン酸Ｓ−ベンジＡＯ９０−ジ
イソプロピＡ（以ＦｒＩＢＰＪという）との２種混合物
％−有効成分として含有するととＶ特徴とする貴業用殺
園組成物に関し、とくにイネもち病およびイネ籾枯細−
病防除に著しい相剰的効果を発揮する新規な＃ｌ業用ｆ
ｉｌ１組成物な提供するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention provides kasugamycin or its salts (hereinafter (-K
Regarding the agrochemical composition for commercial use, which is characterized in that it contains as an active ingredient a two-component mixture of S-bendiAO90-diisopropyl-A (hereinafter referred to as FrIBPJ) and S-bendi-thiophosphoric acid (hereinafter referred to as FrIBPJ), especially for rice blast and rice blast disease. Rice paddy withered and thin
A new #l commercial f that exhibits significant complementary effects in disease control
IL1 compositions are provided.

近年、イネ栽培法の省力化が進み、稚苗機械移植が全国
的に広く普及している。こ１につｒで。In recent years, rice cultivation methods have become more labor-saving, and mechanical transplantation of young seedlings has become widespread nationwide. This one is r.

イネ病害の発生様相にも大きな変化が現われている。こ
とに稚苗機械移植用の箱育苗栽培法は、密播密植の高温
多湿条件で栽培さｊるので、イネ籾枯細１病による直立
枯病や緑化期までの多湿に伴５イネ苗いもち病の多発が
問題視さｎ、るようになり、これの病害に対する新規な
省力的同時防除剤と防除技術の開発が極めて急がれてい
るのが現状である。ζうした殺菌組成物を育苗箱に一用
した場合の病害防除は、省力的であることはもちろんの
乙と、薬剤権用竜が通常散布の６分の１から１０分の１
で十分な病害防除効果な発揮するために経済的防除につ
ながり、また薬剤の多量投与による環境汚染防止の観点
からも極めて望ましい病害防除法である。Significant changes are also appearing in the appearance of rice diseases. In particular, the box seedling cultivation method for mechanical transplantation of young seedlings is cultivated under hot and humid conditions with dense sowing, so rice seedlings can suffer from upright blight due to rice wilt disease 1 and rice seedling rot due to high humidity until the greening stage. The frequent occurrence of diseases has become a problem, and the current situation is that there is an extremely urgent need to develop new labor-saving simultaneous control agents and control techniques for these diseases. Disease control when using the disinfectant composition in seedling boxes is not only labor-saving, but also one-sixth to one-tenth of normal spraying.
It is an extremely desirable disease control method from the viewpoint of economical control since it exhibits sufficient disease control effects, and also from the viewpoint of preventing environmental pollution due to the administration of large amounts of drugs.

本発明の農業用殺菌組成物の有効成分の一つであり　Ｋ
ＳＭ　ｈｘ　、ストレタトイセスヵスガエンシスと命名
された放線１の生産する抗カビ性物質（％公昭４２−６
８１８号明細書ンで、イネいもち病に浸透的な殺菌作用
を示して治療効果に優ｊ１．また人畜や魚貝類に対する
毒性が極めて低く、イネに対すへ薬害もない。そのため
心煽は、安全な地上および空中散布用のイネいもち病防
除剤として広く実用化されている。、また最近において
は、　ＫＳＭがイネ籾枯ｓｌＩ病菌に抗菌活性な示すこ
とが知られてＶる。しかしながら、　ＫＳＭはイネいも
ち病に対して残効性中子防効果に乏しい憾みがある。ま
た最近問題視されているイネ籾枯細菌病菌の侵害による
イネ苗立枯病に対してＫａＭ　＆単独で使用したのでは
経済的な実用効果は期待し難いのが現状である。K is one of the active ingredients of the agricultural fungicidal composition of the present invention.
SM hx, an antifungal substance produced by Actinomyces 1, named Stretatoyces casugaensis (% Kosho 42-6
No. 818 discloses that it exhibits a penetrating bactericidal action against rice blast disease and has excellent therapeutic effects.j1. It also has extremely low toxicity to humans, livestock, fish and shellfish, and has no chemical toxicity to rice. Therefore, Shinban has been widely put into practical use as a safe rice blast control agent for ground and aerial spraying. Recently, it has been known that KSM exhibits antibacterial activity against rice blight slI pathogen. However, KSM has the drawback of lacking residual core protection against rice blast. Furthermore, it is currently difficult to expect an economical practical effect if KaM & is used alone against rice seedling damping-off caused by the invasion of rice blight bacteria, which has recently become a problem.

仁のような事情に鑑み１本発明者ら＆ゴ、現在安全農薬
の代表例として広く認知されている順の長所な生かしつ
つ、かつ前述のような欠点な補足するとともに、イネい
もち病およびイネ籾枯細菌病に対して冑苗箱瘤用と水面
抱用のいずれの方法でも使用でする同時防除剤な開発す
るために多くの化合物な供試し１種々の検討を重ねた。In view of the current circumstances, the present inventors & Go., while taking advantage of the advantages of the plant, which is currently widely recognized as a representative example of safe agricultural chemicals, and supplementing the drawbacks mentioned above, have developed a method to prevent rice blast disease and rice blast disease. In order to develop a simultaneous control agent for rice blight bacterial disease that can be used for both methods of controlling rice seedlings and water surface, we conducted a series of studies using a large number of chemical compounds.

その結果、に８Ｍに既にイネいもち剃切除剤として実用
化されているＩＢＰ　（特公昭４２−３０４０号公報。As a result, IBP (Japanese Patent Publication No. 3040/1983) has already been put into practical use as a rice blast excision agent.

同４７−２４７３１号公報および社団法人日本植物防疫
協会発行の「農薬ハンドブック１９７６年版」の第１６
３ル１６５ 新規な殺菌組成物な使用すらことにより，前記目的が十
分達成できることｔ見出し本発明な完成した。本発明の
農業用膜１組酸物のイネいもち病およびイネ籾枯１１Ａ
＊１ｌｌＫ対する防除効果は，以下の試験例で明らかな
ように，各有効成分の単剤織用では全く見い出すことの
できなかりた極めて優れた防除効果な発揮するはかりで
なく．薬剤間において甚だ高い相乗性な示すという意ｍ
外な事実に起因するものであり１両病害に対する同時防
除剤としての実用性は極めて高い。No. 47-24731 and No. 16 of the "Pesticide Handbook 1976 Edition" published by the Japan Plant Protection Association.
The present invention has been completed based on the finding that the above objects can be fully achieved by using a novel disinfectant composition. Rice blast disease and rice blight 11A of the agricultural membrane 1 set of acid products of the present invention
*As is clear from the test examples below, the control effect against 1llK is not a measure of the extremely excellent control effect that could not be found with the use of each active ingredient alone. This means that there is extremely high synergy between the drugs.
This is due to an external fact, and its practicality as a simultaneous control agent for both diseases is extremely high.

本発明の農業用殺菌組成物な製剤化するＫは有効成分と
常法で使用される担体としては、クレー。The agricultural fungicidal composition of the present invention is formulated using clay as an active ingredient and a conventional carrier.

タルタ，ベントナイト、力すリン、けいそ５±。Taruta, Bentonite, Chikarasurin, Keiso 5±.

シリカなどの固体担体，あるいはベンゼン、１１？シレ
ン，トルエン、ケロシン、アｈコールＩＩＩ（メタノー
ル、エタノール、インプロパノー島，ｎー７’ーノール
など）、ケトンｌｌ（ア七トン、メチルエテルケトン、
シクロへ中ナノン）なとの液体担体が使用される．これ
らに適蟲な界ｌｌ１ｌ’ｉＩ性剤や七の他の補助剤１例
えば安定剤、展着剤など１適量配合して製剤化して使用
できる０本発１１１１における有効成分の配合割合は重
電比でに８Ｍ　Ｉｋ′１部に対しＩＢＰ　Ｉｋ−２．　
５〜５０部配合するのが適蟲であるが。A solid support such as silica, or benzene, 11? Silene, toluene, kerosene, alcohol III (methanol, ethanol, impropanol, n-7'-nol, etc.), ketone II (a-heptone, methyl ether ketone,
Liquid carriers such as cyclonanons are used. The proportion of active ingredients in 1111 can be formulated by adding an appropriate amount of a suitable sex agent or other auxiliary agent such as a stabilizer or a spreading agent to these ingredients. The ratio is 1 part of 8M Ik' to 2 parts of IBP Ik'.
It is appropriate to mix 5 to 50 parts.

捲用時の条件や病害発生状況に応じて配合割合な適宜変
更して使用することができる。オた本発明の農業用殺菌
組成物に他の殺菌剤，殺虫剤，除草剤などな配合して使
用することもできる。The blending ratio can be changed as appropriate depending on the conditions at the time of rolling and the occurrence of disease. In addition, other fungicides, insecticides, herbicides, etc. may be added to the agricultural fungicidal composition of the present invention.

次に本発明の実権例な示す。なお実権例中部とあるのは
全て重量部である６また心ばはすべて塩酸塩である。Next, a practical example of the present invention will be shown. In addition, all numbers in the central part of the actual example are parts by weight (6), and all parts in the middle part are hydrochloride.

実権例】（水和剤） ＫＳＭ　３部，ＩＢＰ２０！ｔ１．ホワイトカーボン１
５部，ツウ１フルサルフエート３部，リグニンスルホシ
サン酸カルシワム５部およびクレー５６部な混合して十
分粉砕して水和剤を得た。Actual example] (hydrating powder) KSM 3 parts, IBP20! t1. white carbon 1
5 parts of Tu-1 fursulfate, 5 parts of calcium lignin sulfosisanoate, and 56 parts of clay were mixed and thoroughly ground to obtain a wettable powder.

本水和剤は．イネいもち病およびイネ籾枯細菌病の発生
前または発生Ｉｋ後に，水で６００〜１０００倍に希釈
し適轟な散布器具で補体上に３０アール当り１００〜１
８０ｔの割合で散布すれば，こｊ。This hydrating agent is. Before or after the outbreak of rice blast disease and rice bacterial blight disease, dilute it 600 to 1000 times with water and spray it on the complement with a suitable spraying device at 100 to 1 1/30 are.
If sprayed at a rate of 80 tons, this will be achieved.

らの諸病害な単独あるいは同時に防除することができる
。Various diseases can be controlled singly or simultaneously.

実織例２（粉剤） ＫＳＭ　Ｏ．　５部，ＩＢＰ］．５部，ホワイトカーボ
ン１．０部，イノプロピ＾アシッドホスフェート（物理
性改良剤）０．５部およびクレー９６．９部９ｆ混合し
て十分粉砕して粉剤を得た。Actual weaving example 2 (powder) KSM O. Part 5, IBP]. 5 parts of white carbon, 1.0 part of white carbon, 0.5 part of inopropyl acid phosphate (physical properties improver), and 96.9 parts of clay, 9f were mixed and sufficiently ground to obtain a powder.

本粉剤は適轟な散布器具を用いて、七〇壕ｔ′ｌＩＡ体
上に１０アーＩ＆邑り１．５〜＋ｋｐの割合で散粉すれ
ば，イネいもち病およびイネ籾枯ｓｓｉ病虻単独あるい
は同時に防除することができる。If this powder is sprinkled on 70 trenches at a rate of 10 ar I & 1.5 to +kp using a suitable spraying device, it will prevent rice blast and rice blight ssi disease alone or It can be controlled at the same time.

実権例３（粒剤） ＫＳＭ　２部，ＩＢＰｇ部，ホワイトカーボン６部。Actual example 3 (granules) KSM 2 parts, IBPg part, white carbon 6 parts.

ドデシルベンゼンスルホン酸ソーダ１部，ベントナイト
１０ｍ１．ｌＪゲニンスルホン酸塩５部およびグレー６
８部なよく混合し粉砕したのち基土の水な加えて練合し
造粒ＩＩにて造粒し乾燥して１０〜４２メッシ．の粒剤
とした。1 part of sodium dodecylbenzenesulfonate, 10ml of bentonite. lJ geninsulfonate 5 parts and gray 6
After thoroughly mixing 8 parts and pulverizing, add water from the base soil, knead, granulate with Granulation II, and dry to form a 10-42 mesh. It was made into granules.

本粒剤け，イネ種苗育苗箱に種籾な播種する直前（培土
混和処理）から機械移植娼日（散粒処理りのいずれかに
育苗箱当り２０〜６ａｌ宛總用すｊ。This granule solution can be applied to 20 to 6 al per seedling box either from just before sowing (mixing soil) to mechanical transplantation day (spraying).

ば、イネいもち病およびイネ籾枯４＠自病な単独あるい
は同時防除することかできる。For example, it is possible to control rice blast and rice blight4@self-infectious diseases either alone or simultaneously.

次に本発明の実業用殺菌組成物の病害防除効果な試験例
により親羽する。Next, a test example of the disease control effect of the industrial fungicidal composition of the present invention will be conducted.

試験例】（育苗箱總用によるイネいもち病害除効果試験
） イネ（品種：コシヒカリ）の乾燥ＩＸＩベンレートＴ水
和剤２０（市販の種子消毒剤）の２００培液で４８時間
浸漬消毒したのち常法により浸漬催芽させたハト胸状態
の種籾な標準育苗箱（３１０ＣＩＩＸ　５　ＱＣＩＩＸ
　３ｃｓ、培土は市販のクレハ粒状培土）に乾粉相当量
で２０ＯＮ宛播種した（５月１日）。Test example] (Test on rice blast disease control effect using seedling raising boxes) Dried rice (variety: Koshihikari) was immersed in a 200% culture solution of IXI Benlate T hydrating agent 20 (commercially available seed disinfectant) for 48 hours and then disinfected. Standard seedling box (310CIIX 5 QCIIX
The seeds were sown on 3cs of commercially available Kureha granular soil (commercially available Kureha granular soil) with an amount equivalent to dry powder (20ON) (May 1st).

出芽処理け３０Ｃの恒ａＷｈに３日間格納し緑化期以降
はビニー＾ハ９ス内で栽培管理した。薬剤処理は本田へ
の稚俗機械移植（５月２０日、畦巾３０１ｘ株間１５（
ｊ１株４〜７本植）の３日前に実ニー例３Ｋしたがい磨
製した供試粒剤な育苗箱に均グ゛１ 、−に手まきした。本田（１区４〇−ンへの施肥量は１
０アール当り堆肥１２００に＃１元ｌ！はＮ　、　Ｐ。After budding, the seeds were stored at a constant temperature of 30C for 3 days, and after the greening period, they were cultivated in a vinyl greenhouse. The chemical treatment was transplanted to Honda by a small machine (May 20th, ridge width 301 x plant spacing 15 (
Three days before planting (4 to 7 plants per plant), the seedlings were sown evenly by hand into a seedling box using the test granules polished according to the actual example 3K. Honda (the amount of fertilizer applied to 40-tons in 1 ward is 1
#1 yuan l for compost 1200 per 0 are! is N, P.

Ｋ各成分量で８嗜、追肥は６月２５日に硫安１５ｋｆｖ
ｍ用した１、またイネいもち病のは場発生な促進させる
ためにイネいもち病菌抱子懸濁液（北−３７３菌株な使
用しその胞子濃［は、光学顕微鏡１５０倍の１視野轟り
７０個である）な噴霧接種した。そして接種５日後の罹
病苗（品種：＃！古稲）な６月１７８に各試験区の３株
間に１＊（１株３本植）宛手植えした。K for each component amount: 8 kfv, supplementary fertilizer on June 25th with ammonium sulfate 15 kfv
In addition, to promote the field outbreak of rice blast, a rice blast fungus spore suspension (Kita-373 strain) was used, and its spore concentration was measured using an optical microscope at 150x magnification. (individuals) were spray inoculated. Then, on June 178, 5 days after inoculation, diseased seedlings (variety: #! old rice) were hand-planted at 1* (3 plants per plant) between 3 plants in each test plot.

発病調査は、集いもち発生盛期の７月２３８に１区３０
株についてのイネいもち病病斑数管調査し、次式により
防除価（−を算出した。The outbreak investigation was carried out in July 238, during the height of the outbreak, in 1 ward, 30
The number of rice blast lesions on the plant was investigated, and the control value (-) was calculated using the following formula.

００ 試験は１区２連制で行った。その結果はＩＩＥ１表のと
おりである。00 The test was conducted in two consecutive sections. The results are shown in Table IIE1.

第　　１　　表 註ｌ）薬剤池用４日ＩＩ（本田稚苗機械移植当日）の褐
点葉先き枯れなどの薬害発生状況：＋が多いほど薬害発
生のはげしいことな示す。Notes to Table 1) Occurrence of chemical damage such as browning and wilting of leaves on 4th day II (on the day of Honda seedling machine transplantation) for chemical ponds: The more +, the more severe the chemical damage.

註２）フロベナゾールは、化学名３−アリ＾オキシー１
，２−ベンゾイソチアゾール−１，１ジオ中クドＶ含有
する市販の殺菌剤を使用した。Note 2) Frobenazole has the chemical name 3-ary^oxy-1
A commercially available fungicide containing Kudo V in , 2-benziisothiazole-1,1dio was used.

註３）　かっこ内の数値は、１０株当りの平均イネいも
ち病病斑数な示す。Note 3) The numbers in parentheses indicate the average number of rice blast lesions per 10 plants.

試験ガ２（イネ籾枯細菌病＠によるイネ苗立枯症防除効
果試験） イネ（品Ｓ：朝日）の乾燥籾を試験ガ１と同様Ｋｍ子消
毒後催芽させた鴇籾（ハト脚状１ｆ）ｔ’あらかじめ培
養して得たイネ籾枯細菌懸濁液〔罹病籾から単個分離し
たイネ籾枯細菌病菌（Ｐｓｅｕｄ億＋ｏｔｎｓｇ１日ｅ
　）　（シ為−ド峰ナス、グルメ−）なジャガイ場半合
成液体培地で３０Ｕ″′ｃ２４時間振とう培養し３ｓｌ
＃当り１０８ケに氷で希釈したもの〕に室温下で３箱蟲
りｌＱＱ＠Ｉ宛喝注温和）？つめた標準育苗＠Ｃ３０ａ
ｍｘ６０ｃｓｔＣ５０ａの３分の１の大きさの暗（２１
０３Ｘ２０ｘ　ｘ　３　ｃｍ　）　Ｋ　７０１宛播樵し
た（７月５日ン。そして３０Ｃの恒温ａに３日間格納し
て出芽処理し、緑化期以降はガラス室（温度２０〜３２
Ｃ）で栽培管理した。薬剤処理は、実癩例１に準じて調
製した供試水和剤の所定濃度薬液な播種時に１箱当り１
５Ｇ＃ｊ宛燗注した。Test moth 2 (rice seedling damping-off control effect test caused by rice bacterial rice blight disease @) Dry rice (product S: Asahi) was germinated after germination after Km seed disinfection as in test moth 1. ) t' Suspension of rice rice blight bacteria obtained by culturing in advance
) (seed eggplant, gourmet) Potato field Semi-synthetic liquid medium was cultured with shaking for 24 hours at 30U"'c and 3sl
#108 diluted with ice] and 3 boxes at room temperature. Packed standard seedling @C30a
mx60cstC50a one-third the size (21
03 x 20 x x 3 cm) K 701 (July 5th).Then, they were stored at a constant temperature of 30C for 3 days to treat germination, and after the greening period they were kept in a glass room (temperature 20-32
Cultivation was managed using C). The chemical treatment was carried out using a predetermined concentration of a chemical solution of a test wettable powder prepared according to Leprosy Example 1.
I sent a hot order to 5G#j.

発病調査は、播種２２日後に１箱の５分の１の菌数につ
（１て、部数枯死菌数および集しよう褐変菌数な調べて
次式により発病度な求め、無処理区の発病度との対比か
ら防除価（１９を算出した。22 days after sowing, the number of bacteria in one-fifth of the box was investigated (first, the number of dead bacteria and the number of browning bacteria collected) was calculated using the following formula, and the degree of disease was calculated using the following formula. The control value (19) was calculated from the comparison with the degree.

その結果は第２表のとおりである。The results are shown in Table 2.

第２表 註１）　かっξ内数値は、無処理区の発病度を示す。Table 2 Note 1) The numbers in parentheses ξ indicate the disease severity in the untreated plot.

試験例３（イネいもち病は場畑苗代防除効果試験）イネ
（品種：サナニレ中）の乾燥籾な試験例１と同様にして
催芽させ種籾な畑−場に投置した畑苗代に条播した（６
月１０日）。病ｍｓ種は、自然発病の認められる以前の
イネの３．５葉期時にあらかじめ用意したイネいもち罹
病集の細断片を畑は珈全面に均一にばらまき、−夜ビニ
ールで被覆して発病の促進をはかった。薬剤散布は、実
織例１に準じて調製した供試薬水利剤の所定濃度薬液な
各区１０（１７宛小型自動噴Ｓ＊を用いて病１接種の３
日前（６月２８日）と３日後（７月４日）の２回行った
。Test Example 3 (Efficacy test for controlling rice blast disease on field and seedlings) Dry rice (variety: Sana Elm Medium) was germinated in the same manner as in Test Example 1, and seed paddy was seeded in rows on field seedlings placed in the field ( 6
10th of the month). For diseased species, at the 3.5-leaf stage of rice, before the natural onset of disease, small pieces of a rice blast disease collection prepared in advance are uniformly scattered over the entire surface of the rice field, and then covered with plastic at night to promote disease onset. I measured it. Chemical spraying was carried out using a small automatic sprayer S* for each area 10 (17) with a predetermined concentration of a test water solution prepared according to Example 1.
I went there twice, one day before (June 28th) and three days later (July 4th).

発病調査は、篤２回目の薬剤散布の２４日ｉにイネいも
ち病病斑枯死面積歩合（９１９ＴＩｋＭ査し１次式によ
り防除価（＠を算出した。The disease onset investigation was carried out on the 24th day i after the second chemical spraying, by examining the ratio of dead area of rice blast lesions (919 TIkM) and calculating the control value (@) using a linear formula.

×１００ その結果は第３表のとおりであるや １１３表 註ｌ）フサライドは、４，５，６．７−テトラタロロフ
タリドな示す。×100 The results are shown in Table 3. Table 113 Note l) The fusaride is 4,5,6.7-tetratalolophthalide.

１１Ｅ２）　　かっこ内数値は、無散布区の平均イネい
もち病病斑枯死面積歩合（＠％’示す。11E2) The numbers in parentheses indicate the average rice blast disease spot dead area percentage (@%') in non-sprayed plots.

Claims (1)

【特許請求の範囲】[Claims] カスガマイシンまたはその塩類とデオリン酸Ｓ−ベンジ
ル０，０−ジイソプロピルこの２種混合物な有効成分と
して含有することな特徴とする農業用殺菌組成物。An agricultural fungicidal composition characterized by containing a mixture of kasugamycin or its salts and S-benzyl 0,0-diisopropyl deolate as active ingredients.