JPS5799600A - Preparation of high purity cholesterol - Google Patents
Preparation of high purity cholesterolInfo
- Publication number
- JPS5799600A JPS5799600A JP17618180A JP17618180A JPS5799600A JP S5799600 A JPS5799600 A JP S5799600A JP 17618180 A JP17618180 A JP 17618180A JP 17618180 A JP17618180 A JP 17618180A JP S5799600 A JPS5799600 A JP S5799600A
- Authority
- JP
- Japan
- Prior art keywords
- lpd
- mouse
- cholesterol
- extracted
- high purity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Steroid Compounds (AREA)
Abstract
PURPOSE: To prepare cholesterol in high purity, with extremely simple operation, by extracting the organs from a mouse afflicted with hyperliposis caused by a novel mutant gene, and extracting the organ with a solvent.
CONSTITUTION: A mouse afflicted with hyperliposis caused by a novel munant gene (lpd) is grown, and slaughtered. The organs are extracted from the body and homogenized. Cholesterol is extracted from the product and purified by conventional method. For example, a normal female mouse is transplanted with the ovary of the lpd homozygote, and is mated with a lpd/+male mouse to obtain the lpd/lpd bodies and the lpd/+bodies at a ratio of 1:1. The process is repeated to produce the lpd homozygote. Thereafter, the acetone-soluble fraction is extracted by acetone fractionation process, purified, by silica gel column chromatography, and recrystallized to obtain cholesterol having a purity of ≥99%.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17618180A JPS5799600A (en) | 1980-12-12 | 1980-12-12 | Preparation of high purity cholesterol |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17618180A JPS5799600A (en) | 1980-12-12 | 1980-12-12 | Preparation of high purity cholesterol |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5799600A true JPS5799600A (en) | 1982-06-21 |
JPS6210518B2 JPS6210518B2 (en) | 1987-03-06 |
Family
ID=16009066
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17618180A Granted JPS5799600A (en) | 1980-12-12 | 1980-12-12 | Preparation of high purity cholesterol |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5799600A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102617687A (en) * | 2012-03-07 | 2012-08-01 | 南京工业大学 | Process for preparing high-purity cholesterol |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03191607A (en) * | 1989-12-21 | 1991-08-21 | Seiko Electronic Components Ltd | Thickness slip crystal resonator |
JPH03291010A (en) * | 1990-04-06 | 1991-12-20 | Seiko Electronic Components Ltd | Thickness-shear crystal resonator |
JPH03291011A (en) * | 1990-04-06 | 1991-12-20 | Seiko Electronic Components Ltd | Short-side crystal resonator |
JP3025841U (en) * | 1995-06-27 | 1996-06-25 | スリーアイプロダクツ有限会社 | Foldable stereo photo viewer |
-
1980
- 1980-12-12 JP JP17618180A patent/JPS5799600A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102617687A (en) * | 2012-03-07 | 2012-08-01 | 南京工业大学 | Process for preparing high-purity cholesterol |
Also Published As
Publication number | Publication date |
---|---|
JPS6210518B2 (en) | 1987-03-06 |
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