JPS5736997A - Determination of activity of angiotensinase - Google Patents
Determination of activity of angiotensinaseInfo
- Publication number
- JPS5736997A JPS5736997A JP11176180A JP11176180A JPS5736997A JP S5736997 A JPS5736997 A JP S5736997A JP 11176180 A JP11176180 A JP 11176180A JP 11176180 A JP11176180 A JP 11176180A JP S5736997 A JPS5736997 A JP S5736997A
- Authority
- JP
- Japan
- Prior art keywords
- nadph
- angiotensinase
- activity
- hippuricase
- histidyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine the activity of the titled enzyme, easily, by mixing p-hydroxy-hippuryl-L-histidyl-L-leucine, hippuricase, NADPH, etc. to the specimen, and determining the consumed NADPH by the measurement of absorbancy.
CONSTITUTION: A liquid specimen containing angiotensinase, e.g. blood serum, body fluid, etc., is mixed with a solution contaning p-hydroxyhippuryl-L-histidyl-L- leucine of formula, hippuricase, p-hydroxybenzoic acid hydroxylase, and NADPH (nicotinamide-adenine dinucleotide phosphate, reduced form), and the consumed NADPH is determined by measuring the decrease of NADPH absorbancy with ultraviolet light or fluorescence.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11176180A JPS5935594B2 (en) | 1980-08-15 | 1980-08-15 | Angiotensin converting enzyme activity measurement method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11176180A JPS5935594B2 (en) | 1980-08-15 | 1980-08-15 | Angiotensin converting enzyme activity measurement method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5736997A true JPS5736997A (en) | 1982-02-27 |
JPS5935594B2 JPS5935594B2 (en) | 1984-08-29 |
Family
ID=14569514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11176180A Expired JPS5935594B2 (en) | 1980-08-15 | 1980-08-15 | Angiotensin converting enzyme activity measurement method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5935594B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108181460A (en) * | 2017-12-22 | 2018-06-19 | 苏州博源医疗科技有限公司 | Dipeptidase derivant for angiotensinⅠ detection and its preparation method and application |
-
1980
- 1980-08-15 JP JP11176180A patent/JPS5935594B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108181460A (en) * | 2017-12-22 | 2018-06-19 | 苏州博源医疗科技有限公司 | Dipeptidase derivant for angiotensinⅠ detection and its preparation method and application |
Also Published As
Publication number | Publication date |
---|---|
JPS5935594B2 (en) | 1984-08-29 |
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