JPS57154058A - Determining method of calcium - Google Patents

Determining method of calcium

Info

Publication number
JPS57154058A
JPS57154058A JP4055581A JP4055581A JPS57154058A JP S57154058 A JPS57154058 A JP S57154058A JP 4055581 A JP4055581 A JP 4055581A JP 4055581 A JP4055581 A JP 4055581A JP S57154058 A JPS57154058 A JP S57154058A
Authority
JP
Japan
Prior art keywords
calcium
orthocresolphthaleincomplexon
coloration
citrate
citric acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4055581A
Other languages
Japanese (ja)
Inventor
Kazuhiko Yamanishi
Toshiro Hanada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP4055581A priority Critical patent/JPS57154058A/en
Publication of JPS57154058A publication Critical patent/JPS57154058A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To determine the calcium in an organism sample simply, quickly, and accurately, by providng the coexistence state by putting citric acid or citrate in orthocresolphthaleincomplexon, and measuring the coloration of the calcium. CONSTITUTION:The calcium in the organism sample is determined by measuring the coloration of the orthocresolphthaleincomplexon and the calcium. In this method, the reagent, which is prepared in such a way that the concentration of the citric acid, citrate, or their mixture becomes preferably about 0.01M- 0.05M/l, is used in the coloration reaction of the orthocresolphthaleincomplexon and the calcium, and the calcium in the organism sample is determined. As for the calcium determining reagent wherein orthocresolphthaleincomplexon is used as a coloring agent, since the reagent composition itself, which is usually employed, can be used in a intact state, the circuit acid, the citrate, or their mixture can be added in the final coloration liquid so that concentration of the citric acid becomes 0.01-0.05M/l.
JP4055581A 1981-03-20 1981-03-20 Determining method of calcium Pending JPS57154058A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4055581A JPS57154058A (en) 1981-03-20 1981-03-20 Determining method of calcium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4055581A JPS57154058A (en) 1981-03-20 1981-03-20 Determining method of calcium

Publications (1)

Publication Number Publication Date
JPS57154058A true JPS57154058A (en) 1982-09-22

Family

ID=12583689

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4055581A Pending JPS57154058A (en) 1981-03-20 1981-03-20 Determining method of calcium

Country Status (1)

Country Link
JP (1) JPS57154058A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61247969A (en) * 1985-04-24 1986-11-05 メルク・パテント・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method of analyzing calcium and reagent for analysis thereof
JP2020514716A (en) * 2016-12-27 2020-05-21 ヒルズ・ペット・ニュートリシャン・インコーポレーテッド Calcium oxalate titration test kit and pet food composition

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61247969A (en) * 1985-04-24 1986-11-05 メルク・パテント・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method of analyzing calcium and reagent for analysis thereof
JP2020514716A (en) * 2016-12-27 2020-05-21 ヒルズ・ペット・ニュートリシャン・インコーポレーテッド Calcium oxalate titration test kit and pet food composition
US11330830B2 (en) 2016-12-27 2022-05-17 Hills Pet Nutrition, Inc. Calcium oxalate titration test kits and pet food compositions

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