JPS5692790A - Preparation of sarcosine oxidase - Google Patents

Preparation of sarcosine oxidase

Info

Publication number
JPS5692790A
JPS5692790A JP16986079A JP16986079A JPS5692790A JP S5692790 A JPS5692790 A JP S5692790A JP 16986079 A JP16986079 A JP 16986079A JP 16986079 A JP16986079 A JP 16986079A JP S5692790 A JPS5692790 A JP S5692790A
Authority
JP
Japan
Prior art keywords
cylindrocarpon
sarcosine oxidase
didymum
sarcosine
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP16986079A
Other languages
Japanese (ja)
Other versions
JPS6243670B2 (en
Inventor
Hideaki Yamada
Yoshiki Tani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP16986079A priority Critical patent/JPS5692790A/en
Publication of JPS5692790A publication Critical patent/JPS5692790A/en
Publication of JPS6243670B2 publication Critical patent/JPS6243670B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare the titled enzyme usable for the determination of creatinine and creatine in a large quantity, by cultivating Cylindrocarpon didymum M-1.
CONSTITUTION: A microorganism which belongs to the genus Cylindrocarpon and has the ability to produce sarcosine oxidase, e.g., cylindrocarpon didymum M-1, is cultivated in an ordinary culture medium containing a carbon and a nitrogen sources, an inorganic salt, etc. The addition of choline, dimethylglycine or sarcosine to the culture medium further improves the productivity of the sarcosine oxidase. After the aerobic cultivation, the aimed substance is obtained by the centrifugation, ultrasonic treatment, milling in a homogenizer, fractionation with ammonium sulfate, ion exchange chromatography, gel filtration method, etc.
COPYRIGHT: (C)1981,JPO&Japio
JP16986079A 1979-12-26 1979-12-26 Preparation of sarcosine oxidase Granted JPS5692790A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16986079A JPS5692790A (en) 1979-12-26 1979-12-26 Preparation of sarcosine oxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16986079A JPS5692790A (en) 1979-12-26 1979-12-26 Preparation of sarcosine oxidase

Publications (2)

Publication Number Publication Date
JPS5692790A true JPS5692790A (en) 1981-07-27
JPS6243670B2 JPS6243670B2 (en) 1987-09-16

Family

ID=15894275

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16986079A Granted JPS5692790A (en) 1979-12-26 1979-12-26 Preparation of sarcosine oxidase

Country Status (1)

Country Link
JP (1) JPS5692790A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740465A (en) * 1985-01-11 1988-04-26 Noda Institute For Scientific Research Heat-resistant sarcosine oxidase N and process for producing the same
US4743549A (en) * 1985-05-29 1988-05-10 Boehringer Mannheim Gmbh Hydrogen peroxide-forming sarcosine oxidase
WO2004044193A1 (en) 2002-11-13 2004-05-27 Toyo Boseki Kabushiki Kaisha Modified sarcosine oxidase, process for producing the same and reagent composition using the same
US7132253B2 (en) 2003-11-18 2006-11-07 Kikkoman Corporation Modified sarcosine oxidases, modified sarcosine oxidase genes, and methods for preparing the modified sarcosine oxidases
US11479757B2 (en) 2016-09-15 2022-10-25 Kikkoman Corporation Modified sarcosine oxidase, and gene and production method therefor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740465A (en) * 1985-01-11 1988-04-26 Noda Institute For Scientific Research Heat-resistant sarcosine oxidase N and process for producing the same
US4743549A (en) * 1985-05-29 1988-05-10 Boehringer Mannheim Gmbh Hydrogen peroxide-forming sarcosine oxidase
WO2004044193A1 (en) 2002-11-13 2004-05-27 Toyo Boseki Kabushiki Kaisha Modified sarcosine oxidase, process for producing the same and reagent composition using the same
US7132253B2 (en) 2003-11-18 2006-11-07 Kikkoman Corporation Modified sarcosine oxidases, modified sarcosine oxidase genes, and methods for preparing the modified sarcosine oxidases
US11479757B2 (en) 2016-09-15 2022-10-25 Kikkoman Corporation Modified sarcosine oxidase, and gene and production method therefor

Also Published As

Publication number Publication date
JPS6243670B2 (en) 1987-09-16

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